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src="https://0.academia-photos.com/25381401/73984592/62474411/s200_ranga.sampath.jpeg" /></div><div class="title-container"><h1 class="ds2-5-heading-sans-serif-sm">Ranga Sampath</h1><div class="affiliations-container fake-truncate js-profile-affiliations"></div></div></div><div class="sidebar-cta-container"><button class="ds2-5-button hidden profile-cta-button grow js-profile-follow-button" data-broccoli-component="user-info.follow-button" data-click-track="profile-user-info-follow-button" data-follow-user-fname="Ranga" data-follow-user-id="25381401" data-follow-user-source="profile_button" data-has-google="false"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">add</span>Follow</button><button class="ds2-5-button hidden profile-cta-button grow js-profile-unfollow-button" data-broccoli-component="user-info.unfollow-button" data-click-track="profile-user-info-unfollow-button" data-unfollow-user-id="25381401"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">done</span>Following</button></div></div><div class="user-stats-container"><a><div class="stat-container js-profile-followers"><p class="label">Followers</p><p class="data">7</p></div></a><a><div class="stat-container js-profile-followees" data-broccoli-component="user-info.followees-count" data-click-track="profile-expand-user-info-following"><p class="label">Following</p><p class="data">7</p></div></a><span><div class="stat-container"><p class="label"><span class="js-profile-total-view-text">Public Views</span></p><p class="data"><span class="js-profile-view-count"></span></p></div></span></div><div class="user-bio-container"><div class="profile-bio fake-truncate js-profile-about" style="margin: 0px;"><b>Address: </b>San Diego, California, United States<br /><div class="js-profile-less-about u-linkUnstyled u-tcGrayDarker u-textDecorationUnderline u-displayNone">less</div></div></div><div class="ri-section"><div class="ri-section-header"><span>Interests</span></div><div class="ri-tags-container"><a data-click-track="profile-user-info-expand-research-interests" data-has-card-for-ri-list="25381401" href="https://www.academia.edu/Documents/in/Politics_of_Secularism"><div id="js-react-on-rails-context" style="display:none" data-rails-context="{"inMailer":false,"i18nLocale":"en","i18nDefaultLocale":"en","href":"https://independent.academia.edu/RangaSampath","location":"/RangaSampath","scheme":"https","host":"independent.academia.edu","port":null,"pathname":"/RangaSampath","search":null,"httpAcceptLanguage":null,"serverSide":false}"></div> <div class="js-react-on-rails-component" style="display:none" data-component-name="Pill" data-props="{"color":"gray","children":["Politics of Secularism"]}" data-trace="false" data-dom-id="Pill-react-component-e356b1b4-2671-45ce-b99e-67142412d2e2"></div> <div id="Pill-react-component-e356b1b4-2671-45ce-b99e-67142412d2e2"></div> </a><a 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data-click-track="profile-user-info-expand-research-interests" data-has-card-for-ri-list="25381401" href="https://www.academia.edu/Documents/in/Contemporary_International_Migration"><div class="js-react-on-rails-component" style="display:none" data-component-name="Pill" data-props="{"color":"gray","children":["Contemporary International Migration"]}" data-trace="false" data-dom-id="Pill-react-component-fe573cf5-efda-4a75-8a2b-73a73c7594bd"></div> <div id="Pill-react-component-fe573cf5-efda-4a75-8a2b-73a73c7594bd"></div> </a><a data-click-track="profile-user-info-expand-research-interests" data-has-card-for-ri-list="25381401" href="https://www.academia.edu/Documents/in/Racial_and_ethnic_discrimination"><div class="js-react-on-rails-component" style="display:none" data-component-name="Pill" data-props="{"color":"gray","children":["Racial and ethnic discrimination"]}" data-trace="false" data-dom-id="Pill-react-component-d65a1e45-8b3a-4aa5-b875-e31d7cce8089"></div> <div id="Pill-react-component-d65a1e45-8b3a-4aa5-b875-e31d7cce8089"></div> </a></div></div><div class="external-links-container"><ul class="profile-links new-profile js-UserInfo-social"><li class="profile-profiles js-social-profiles-container"><i class="fa fa-spin fa-spinner"></i></li></ul></div></div></div><div class="right-panel-container"><div class="user-content-wrapper"><div class="uploads-container" id="social-redesign-work-container"><div class="upload-header"><h2 class="ds2-5-heading-sans-serif-xs">Uploads</h2></div><div class="documents-container backbone-social-profile-documents" style="width: 100%;"><div class="u-taCenter"></div><div class="profile--tab_content_container js-tab-pane tab-pane active" id="all"><div class="profile--tab_heading_container js-section-heading" data-section="Papers" id="Papers"><h3 class="profile--tab_heading_container">Papers by Ranga Sampath</h3></div><div class="js-work-strip profile--work_container" data-work-id="95254934"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/95254934/Modulation_of_molecular_interaction_positions_in_rns_and_other_biomolecules"><img alt="Research paper thumbnail of Modulation of molecular interaction positions in rns and other biomolecules" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/95254934/Modulation_of_molecular_interaction_positions_in_rns_and_other_biomolecules">Modulation of molecular interaction positions in rns and other biomolecules</a></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span 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observational study" class="work-thumbnail" src="https://attachments.academia-assets.com/97486994/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/95254933/The_detection_of_microbial_DNA_but_not_cultured_bacteria_is_associated_with_increased_mortality_in_patients_with_suspected_sepsis_a_prospective_multi_centre_European_observational_study">The detection of microbial DNA but not cultured bacteria is associated with increased mortality in patients with suspected sepsis—a prospective multi-centre European observational study</a></div><div class="wp-workCard_item"><span>Clinical Microbiology and Infection</span><span>, 2017</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="8aa7c9f943e43336750b34ca357f70b5" 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href="https://www.academia.edu/95254919/S08_1_Diagnostic_Infectious_diseases_self_testing_and_self_sampling_outside_clinics_A_global_systematic_review">S08.1 Diagnostic Infectious diseases self-testing and self-sampling outside clinics: A global systematic review</a></div><div class="wp-workCard_item"><span>Symposium presentations</span><span>, 2021</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="5998c46e1e445d4699b17ed7c62c4f76" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":97486986,"asset_id":95254919,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/97486986/download_file?st=MTczMjQyMjk1Myw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span 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Positive aspects of care include support and the provision of appropriate services from community led and implemented interventions. Recommendations are made in relation to reactions to an HIV positive diagnosis, to support disclosure, address structural impediments in and outside of the health facilities. Recommendations are also made to support retention in care by addressing issues specifically to the health facility.","publication_date":{"day":null,"month":null,"year":2021,"errors":{}},"publication_name":"Symposium presentations","grobid_abstract_attachment_id":97486986},"translated_abstract":null,"internal_url":"https://www.academia.edu/95254919/S08_1_Diagnostic_Infectious_diseases_self_testing_and_self_sampling_outside_clinics_A_global_systematic_review","translated_internal_url":"","created_at":"2023-01-18T13:35:36.944-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":25381401,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":97486986,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/97486986/thumbnails/1.jpg","file_name":"A10.3.full.pdf","download_url":"https://www.academia.edu/attachments/97486986/download_file?st=MTczMjQyMjk1Myw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"S08_1_Diagnostic_Infectious_diseases_sel.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/97486986/A10.3.full-libre.pdf?1674080870=\u0026response-content-disposition=attachment%3B+filename%3DS08_1_Diagnostic_Infectious_diseases_sel.pdf\u0026Expires=1732426553\u0026Signature=QSz2~nRD2PP0UaMJRLuz9gD3Rrn7IXMwKfdItug5quEg~mVS~0bxL~emDPH3cJhYxvA-zXjSnGCXrOyOyYLpzP2FJ7MbEUCaRsICgS2mV3dTyLrh2T2zbuVJvAC7phfY7IdRVnosE0DxyI~nAuInBqbvtSG32KPLVxEYKXqJ~alLm28W~hE4OPoDHvqs2gsmo-c5hlt3N6dK-nyzvHZIyMq6-gm-HxJWZbofmaan2LgSnQ1o3kDCN~o1-KAWkhrqhODI2Lq1GB7S6UlhjlxfjsyOswSMC4~jfkxnLoDnKlKHhehnibgAi5Cy-m7H44hUBO56it0cpYtRghe-xWsxRA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"S08_1_Diagnostic_Infectious_diseases_self_testing_and_self_sampling_outside_clinics_A_global_systematic_review","translated_slug":"","page_count":2,"language":"en","content_type":"Work","owner":{"id":25381401,"first_name":"Ranga","middle_initials":null,"last_name":"Sampath","page_name":"RangaSampath","domain_name":"independent","created_at":"2015-01-26T21:28:55.724-08:00","display_name":"Ranga Sampath","url":"https://independent.academia.edu/RangaSampath"},"attachments":[{"id":97486986,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/97486986/thumbnails/1.jpg","file_name":"A10.3.full.pdf","download_url":"https://www.academia.edu/attachments/97486986/download_file?st=MTczMjQyMjk1Myw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"S08_1_Diagnostic_Infectious_diseases_sel.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/97486986/A10.3.full-libre.pdf?1674080870=\u0026response-content-disposition=attachment%3B+filename%3DS08_1_Diagnostic_Infectious_diseases_sel.pdf\u0026Expires=1732426553\u0026Signature=QSz2~nRD2PP0UaMJRLuz9gD3Rrn7IXMwKfdItug5quEg~mVS~0bxL~emDPH3cJhYxvA-zXjSnGCXrOyOyYLpzP2FJ7MbEUCaRsICgS2mV3dTyLrh2T2zbuVJvAC7phfY7IdRVnosE0DxyI~nAuInBqbvtSG32KPLVxEYKXqJ~alLm28W~hE4OPoDHvqs2gsmo-c5hlt3N6dK-nyzvHZIyMq6-gm-HxJWZbofmaan2LgSnQ1o3kDCN~o1-KAWkhrqhODI2Lq1GB7S6UlhjlxfjsyOswSMC4~jfkxnLoDnKlKHhehnibgAi5Cy-m7H44hUBO56it0cpYtRghe-xWsxRA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":108566,"name":"Sexually transmitted infections","url":"https://www.academia.edu/Documents/in/Sexually_transmitted_infections"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="70138687"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/70138687/Genotypic_Variation_and_Mixtures_of_Lyme_Borrelia_in_Ixodes_Ticks_from_North_America_and_Europe"><img alt="Research paper thumbnail of Genotypic Variation and Mixtures of Lyme Borrelia in Ixodes Ticks from North America and Europe" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/70138687/Genotypic_Variation_and_Mixtures_of_Lyme_Borrelia_in_Ixodes_Ticks_from_North_America_and_Europe">Genotypic Variation and Mixtures of Lyme Borrelia in Ixodes Ticks from North America and Europe</a></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Background: Lyme disease, caused by various species of Borrelia, is transmitted by Ixodes ticks i...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Background: Lyme disease, caused by various species of Borrelia, is transmitted by Ixodes ticks in North America and Europe. Studies have shown the genotype of Borrelia burgdorferi sensu stricto (s.s.) or the species of B. burgdorferi sensu lato (s.l.) affects the ability of the bacteria to cause local or disseminated infection in humans. Methodology/Principal Findings: We used a multilocus PCR electrospray mass spectrometry assay to determine the species and genotype Borrelia from ticks collected in New York, Connecticut, Indiana, Southern Germany, and California and characterized isolates from parts of the United States and Europe. These analyses identified 53 distinct genotypes of B. burgdorferi sensu stricto with higher resolution than ospC typing. Genotypes of other members of the B. burgdorferi sensu lato complex were also identified and genotyped including B. afzelii, B. garinii, B. lusitaniae, B. spielmanii, and B. valaisiana. While each site in North America had genotypes u...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="70138687"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="70138687"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 70138687; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=70138687]").text(description); $(".js-view-count[data-work-id=70138687]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 70138687; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='70138687']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 70138687, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=70138687]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":70138687,"title":"Genotypic Variation and Mixtures of Lyme Borrelia in Ixodes Ticks from North America and Europe","translated_title":"","metadata":{"abstract":"Background: Lyme disease, caused by various species of Borrelia, is transmitted by Ixodes ticks in North America and Europe. Studies have shown the genotype of Borrelia burgdorferi sensu stricto (s.s.) or the species of B. burgdorferi sensu lato (s.l.) affects the ability of the bacteria to cause local or disseminated infection in humans. Methodology/Principal Findings: We used a multilocus PCR electrospray mass spectrometry assay to determine the species and genotype Borrelia from ticks collected in New York, Connecticut, Indiana, Southern Germany, and California and characterized isolates from parts of the United States and Europe. These analyses identified 53 distinct genotypes of B. burgdorferi sensu stricto with higher resolution than ospC typing. Genotypes of other members of the B. burgdorferi sensu lato complex were also identified and genotyped including B. afzelii, B. garinii, B. lusitaniae, B. spielmanii, and B. valaisiana. While each site in North America had genotypes u...","publication_date":{"day":null,"month":null,"year":2013,"errors":{}}},"translated_abstract":"Background: Lyme disease, caused by various species of Borrelia, is transmitted by Ixodes ticks in North America and Europe. Studies have shown the genotype of Borrelia burgdorferi sensu stricto (s.s.) or the species of B. burgdorferi sensu lato (s.l.) affects the ability of the bacteria to cause local or disseminated infection in humans. Methodology/Principal Findings: We used a multilocus PCR electrospray mass spectrometry assay to determine the species and genotype Borrelia from ticks collected in New York, Connecticut, Indiana, Southern Germany, and California and characterized isolates from parts of the United States and Europe. These analyses identified 53 distinct genotypes of B. burgdorferi sensu stricto with higher resolution than ospC typing. Genotypes of other members of the B. burgdorferi sensu lato complex were also identified and genotyped including B. afzelii, B. garinii, B. lusitaniae, B. spielmanii, and B. valaisiana. While each site in North America had genotypes u...","internal_url":"https://www.academia.edu/70138687/Genotypic_Variation_and_Mixtures_of_Lyme_Borrelia_in_Ixodes_Ticks_from_North_America_and_Europe","translated_internal_url":"","created_at":"2022-01-31T04:22:39.339-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":25381401,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Genotypic_Variation_and_Mixtures_of_Lyme_Borrelia_in_Ixodes_Ticks_from_North_America_and_Europe","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":25381401,"first_name":"Ranga","middle_initials":null,"last_name":"Sampath","page_name":"RangaSampath","domain_name":"independent","created_at":"2015-01-26T21:28:55.724-08:00","display_name":"Ranga Sampath","url":"https://independent.academia.edu/RangaSampath"},"attachments":[],"research_interests":[{"id":48,"name":"Engineering","url":"https://www.academia.edu/Documents/in/Engineering"},{"id":261,"name":"Geography","url":"https://www.academia.edu/Documents/in/Geography"},{"id":523,"name":"Chemistry","url":"https://www.academia.edu/Documents/in/Chemistry"},{"id":5769,"name":"Mass Spectrometry","url":"https://www.academia.edu/Documents/in/Mass_Spectrometry"},{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":28235,"name":"Multidisciplinary","url":"https://www.academia.edu/Documents/in/Multidisciplinary"},{"id":53198,"name":"Lyme disease","url":"https://www.academia.edu/Documents/in/Lyme_disease"},{"id":64568,"name":"Humans","url":"https://www.academia.edu/Documents/in/Humans"},{"id":75826,"name":"Europe","url":"https://www.academia.edu/Documents/in/Europe"},{"id":99234,"name":"Animals","url":"https://www.academia.edu/Documents/in/Animals"},{"id":128172,"name":"Borrelia burgdorferi","url":"https://www.academia.edu/Documents/in/Borrelia_burgdorferi"},{"id":361848,"name":"Ixodes ricinus","url":"https://www.academia.edu/Documents/in/Ixodes_ricinus"},{"id":372410,"name":"Genotype","url":"https://www.academia.edu/Documents/in/Genotype"},{"id":577933,"name":"Genetic variation","url":"https://www.academia.edu/Documents/in/Genetic_variation"},{"id":756265,"name":"Borrelia","url":"https://www.academia.edu/Documents/in/Borrelia"},{"id":897281,"name":"Ixodes scapularis","url":"https://www.academia.edu/Documents/in/Ixodes_scapularis"},{"id":2457889,"name":"Genotypic diversity","url":"https://www.academia.edu/Documents/in/Genotypic_diversity"},{"id":2693601,"name":"Genotypic variation","url":"https://www.academia.edu/Documents/in/Genotypic_variation"},{"id":3114383,"name":"Ixodes","url":"https://www.academia.edu/Documents/in/Ixodes"}],"urls":[{"id":17157860,"url":"http://citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.354.3158\u0026rep=rep1\u0026type=pdf"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="70138686"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/70138686/Rapid_Detection_and_Characterization_of_the_NAP_1_Epidemic_Strain_of_Clostridium_difficile_Using_PCR_ESI_MS"><img alt="Research paper thumbnail of Rapid Detection and Characterization of the NAP-1 Epidemic Strain of Clostridium difficile Using PCR/ESI-MS" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/70138686/Rapid_Detection_and_Characterization_of_the_NAP_1_Epidemic_Strain_of_Clostridium_difficile_Using_PCR_ESI_MS">Rapid Detection and Characterization of the NAP-1 Epidemic Strain of Clostridium difficile Using PCR/ESI-MS</a></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">ABSTRACT Background: Increasing rate and severity of C. difficile -associated disease (CDAD) have...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">ABSTRACT Background: Increasing rate and severity of C. difficile -associated disease (CDAD) have been associated with the emergence of the NAP-1 strain of C. difficile. Enhanced virulence is associated with deletions within the pathogenicity locus gene, tcdC. We hypothesize that previously known and as yet unknown mutations in the tcd gene cluster may be important for fully understanding CDAD. Methods: We have developed a PCR Electrospray Ionization Mass Spectrometry (PCR/ESI-MS) based method to rapidly identify C. difficile and detect multiple mutations in the tcdC locus, including the previously described 18 base pair deletion and single nucleotide deletion associated with NAP-1 strains, while simultaneously detecting tcdA, tcdB, cdtA and cdtB. Results: PCR/ESI-MS was performed on 65 archived C. difficile clinical isolates that had been collected during previous studies at JHH. 28 of the 65 isolates showed distinct NAP-1 like deletions in the tcdC loci. All 28 had both the 18 bp deletion and an additional single base pair deletion in the promoter region characteristic of fully virulent strains. Two distinct genotypes were observed in the tcdB locus- all 28 virulent strains had a C to T SNP that has not been previously reported. Overall, of the 65 isolates tested, 54 were tcdA+/B+, with 33 showing the tcdB SNP. 4 of the 5 remaining isolates with this SNP had the previously described 36 bp deletion with unknown phenotype in the tcdC locus. Conclusion: The PCR/ESI-MS technology is a high throughput assay system useful for rapid detection and characterization of CDAD. It is capable of simultaneous identification of C. difficile and detection of several important genotypic markers that might be associated with virulence. In addition to detecting all previously reported mutations, it has the potential to uncover additional mutations or correlations that might help understand the virulence properties of this microbe.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="70138686"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="70138686"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 70138686; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=70138686]").text(description); $(".js-view-count[data-work-id=70138686]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 70138686; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='70138686']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 70138686, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=70138686]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":70138686,"title":"Rapid Detection and Characterization of the NAP-1 Epidemic Strain of Clostridium difficile Using PCR/ESI-MS","translated_title":"","metadata":{"abstract":"ABSTRACT Background: Increasing rate and severity of C. difficile -associated disease (CDAD) have been associated with the emergence of the NAP-1 strain of C. difficile. Enhanced virulence is associated with deletions within the pathogenicity locus gene, tcdC. We hypothesize that previously known and as yet unknown mutations in the tcd gene cluster may be important for fully understanding CDAD. Methods: We have developed a PCR Electrospray Ionization Mass Spectrometry (PCR/ESI-MS) based method to rapidly identify C. difficile and detect multiple mutations in the tcdC locus, including the previously described 18 base pair deletion and single nucleotide deletion associated with NAP-1 strains, while simultaneously detecting tcdA, tcdB, cdtA and cdtB. Results: PCR/ESI-MS was performed on 65 archived C. difficile clinical isolates that had been collected during previous studies at JHH. 28 of the 65 isolates showed distinct NAP-1 like deletions in the tcdC loci. All 28 had both the 18 bp deletion and an additional single base pair deletion in the promoter region characteristic of fully virulent strains. 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In addition to detecting all previously reported mutations, it has the potential to uncover additional mutations or correlations that might help understand the virulence properties of this microbe.","publication_date":{"day":null,"month":null,"year":2008,"errors":{}}},"translated_abstract":"ABSTRACT Background: Increasing rate and severity of C. difficile -associated disease (CDAD) have been associated with the emergence of the NAP-1 strain of C. difficile. Enhanced virulence is associated with deletions within the pathogenicity locus gene, tcdC. We hypothesize that previously known and as yet unknown mutations in the tcd gene cluster may be important for fully understanding CDAD. Methods: We have developed a PCR Electrospray Ionization Mass Spectrometry (PCR/ESI-MS) based method to rapidly identify C. difficile and detect multiple mutations in the tcdC locus, including the previously described 18 base pair deletion and single nucleotide deletion associated with NAP-1 strains, while simultaneously detecting tcdA, tcdB, cdtA and cdtB. Results: PCR/ESI-MS was performed on 65 archived C. difficile clinical isolates that had been collected during previous studies at JHH. 28 of the 65 isolates showed distinct NAP-1 like deletions in the tcdC loci. All 28 had both the 18 bp deletion and an additional single base pair deletion in the promoter region characteristic of fully virulent strains. Two distinct genotypes were observed in the tcdB locus- all 28 virulent strains had a C to T SNP that has not been previously reported. Overall, of the 65 isolates tested, 54 were tcdA+/B+, with 33 showing the tcdB SNP. 4 of the 5 remaining isolates with this SNP had the previously described 36 bp deletion with unknown phenotype in the tcdC locus. Conclusion: The PCR/ESI-MS technology is a high throughput assay system useful for rapid detection and characterization of CDAD. It is capable of simultaneous identification of C. difficile and detection of several important genotypic markers that might be associated with virulence. In addition to detecting all previously reported mutations, it has the potential to uncover additional mutations or correlations that might help understand the virulence properties of this microbe.","internal_url":"https://www.academia.edu/70138686/Rapid_Detection_and_Characterization_of_the_NAP_1_Epidemic_Strain_of_Clostridium_difficile_Using_PCR_ESI_MS","translated_internal_url":"","created_at":"2022-01-31T04:22:39.239-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":25381401,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Rapid_Detection_and_Characterization_of_the_NAP_1_Epidemic_Strain_of_Clostridium_difficile_Using_PCR_ESI_MS","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":25381401,"first_name":"Ranga","middle_initials":null,"last_name":"Sampath","page_name":"RangaSampath","domain_name":"independent","created_at":"2015-01-26T21:28:55.724-08:00","display_name":"Ranga Sampath","url":"https://independent.academia.edu/RangaSampath"},"attachments":[],"research_interests":[{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"}],"urls":[]}, dispatcherData: dispatcherData }); 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="58685989"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/58685989/Diagnostic_Infectious_Diseases_Testing_Outside_Clinics_A_Global_Systematic_Review_and_Meta_analysis"><img alt="Research paper thumbnail of Diagnostic Infectious Diseases Testing Outside Clinics: A Global Systematic Review and Meta-analysis" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/58685989/Diagnostic_Infectious_Diseases_Testing_Outside_Clinics_A_Global_Systematic_Review_and_Meta_analysis">Diagnostic Infectious Diseases Testing Outside Clinics: A Global Systematic Review and Meta-analysis</a></div><div class="wp-workCard_item"><span>Open Forum Infectious Diseases</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Background Most people around the world do not have access to facility-based diagnostic testing, ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Background Most people around the world do not have access to facility-based diagnostic testing, and the gap in availability of diagnostic tests is a major public health challenge. Self-testing, self-sampling, and institutional testing outside conventional clinical settings are transforming infectious disease diagnostic testing in a wide range of low- and middle-income countries (LMICs). We examined the delivery models of infectious disease diagnostic testing outside clinics to assess the impact on test uptake and linkage to care. Methods We conducted a systematic review and meta-analysis, searching 6 databases and including original research manuscripts comparing testing outside clinics with conventional testing. The main outcomes were test uptake and linkage to care, delivery models, and adverse outcomes. Data from studies with similar interventions and outcomes within thematic areas of interest were pooled, and the quality of evidence was assessed using GRADE. This study was regi...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="58685989"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="58685989"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 58685989; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=58685989]").text(description); $(".js-view-count[data-work-id=58685989]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 58685989; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='58685989']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 58685989, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=58685989]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":58685989,"title":"Diagnostic Infectious Diseases Testing Outside Clinics: A Global Systematic Review and Meta-analysis","translated_title":"","metadata":{"abstract":"Background Most people around the world do not have access to facility-based diagnostic testing, and the gap in availability of diagnostic tests is a major public health challenge. Self-testing, self-sampling, and institutional testing outside conventional clinical settings are transforming infectious disease diagnostic testing in a wide range of low- and middle-income countries (LMICs). We examined the delivery models of infectious disease diagnostic testing outside clinics to assess the impact on test uptake and linkage to care. Methods We conducted a systematic review and meta-analysis, searching 6 databases and including original research manuscripts comparing testing outside clinics with conventional testing. The main outcomes were test uptake and linkage to care, delivery models, and adverse outcomes. Data from studies with similar interventions and outcomes within thematic areas of interest were pooled, and the quality of evidence was assessed using GRADE. This study was regi...","publisher":"Oxford University Press (OUP)","publication_name":"Open Forum Infectious Diseases"},"translated_abstract":"Background Most people around the world do not have access to facility-based diagnostic testing, and the gap in availability of diagnostic tests is a major public health challenge. Self-testing, self-sampling, and institutional testing outside conventional clinical settings are transforming infectious disease diagnostic testing in a wide range of low- and middle-income countries (LMICs). We examined the delivery models of infectious disease diagnostic testing outside clinics to assess the impact on test uptake and linkage to care. Methods We conducted a systematic review and meta-analysis, searching 6 databases and including original research manuscripts comparing testing outside clinics with conventional testing. The main outcomes were test uptake and linkage to care, delivery models, and adverse outcomes. Data from studies with similar interventions and outcomes within thematic areas of interest were pooled, and the quality of evidence was assessed using GRADE. 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An Analysis of Data from PRIMERS-II of the Antibiotic Resistance Leadership Group (ARLG)</a></div><div class="wp-workCard_item"><span>Open Forum Infectious Diseases</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="cc8b81392c4d97ff3e4c5ef5e4d1d738" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":72976709,"asset_id":58685987,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/72976709/download_file?st=MTczMjQyMjk1Myw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="58685987"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="58685987"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 58685987; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=58685987]").text(description); $(".js-view-count[data-work-id=58685987]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 58685987; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='58685987']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 58685987, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "cc8b81392c4d97ff3e4c5ef5e4d1d738" } } $('.js-work-strip[data-work-id=58685987]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":58685987,"title":"608Can Rapid Molecular Diagnostics Assist in the Choice of b-Lactam Antibiotics? 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="58685986"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/58685986/Acute_Respiratory_Viral_Infection_Among_Outpatient_Healthcare_Personnel"><img alt="Research paper thumbnail of Acute Respiratory Viral Infection Among Outpatient Healthcare Personnel" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/58685986/Acute_Respiratory_Viral_Infection_Among_Outpatient_Healthcare_Personnel">Acute Respiratory Viral Infection Among Outpatient Healthcare Personnel</a></div><div class="wp-workCard_item"><span>Open Forum Infectious Diseases</span><span>, 2015</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">ABSTRACT Background: Respiratory disease is a common source of morbidity among healthcare personn...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">ABSTRACT Background: Respiratory disease is a common source of morbidity among healthcare personnel (HCP). However, the causes of acute respiratory illness (ARI) have not been well studied. We assessed the viral causes of respiratory illness among HCP enrolled in a cluster randomized clinical trial at 99 outpatient departments and emergency departments in 7 locations: Baltimore, MD; Denver, CO (3); Houston, TX; New York, NY; and Washington, DC. Methods: During 12 weeks of the 2011-2 (YR1) and 2012-3 (YR2) respiratory seasons, HCP were surveyed for signs and symptoms of ARI. Participants with symptoms were cultured and swabs frozen at -800C. Also, two random swabs were obtained during the intervention period. Samples were tested using a multiplex PCR for 13 viruses (PCR/ESI-MS, Abbott labs). Paired blood samples were obtained prior to the start of the intervention period and 2-weeks post-intervention, then tested for influenza antibodies (&amp;gt;2-fold antibody increase). YR2 serum samples have not been tested. Results: Among 1686 participants (609 YR1; 1077 YR2), 4255 swabs were obtained. To date, 1579 swab samples have been tested (1370 YR1; 209 YR2): 544 symptomatic (335 YR1; 209 YR2) and 1035 asymptomatic. Influenza antibodies were tested in 1292 serum samples. Based on testing, 102 YR1 and 76 YR2 samples revealed the following viral isolates: influenza A (36), influenza B (8), parainfluenza (2), adenovirus (4), coronavirus (78), metapneumovirus (16), rhinovirus (17), and respiratory syncytial virus (RSV, 17) (Figure 1). Four cases of influenza A were identified by serological and viral testing (Figure 2). Conclusion: ARIs are common among HCP with 25% developing symptoms during the respiratory viral season. Among ARI in HCP, 27% had identifiable viral causes – most commonly coronavirus at 44%. This increased in YR2 with identification of rhinovirus. Most interesting is the significant number of asymptomatic HCP (19%) with identifiable virus. Some of these viruses cause significant morbidity to patients and transmission from HCP is critical to patient safety and that of the HCP themselves. *** Figure 1 Figure 2: Results show both serologic and nose/throat sample results. *Rhinovirus not tested. **Data available for 2 sites, asymptomatic data pending. ***NOTE: The data analysis is ongoing - more information will be available at the presentation.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="58685986"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="58685986"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 58685986; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=58685986]").text(description); $(".js-view-count[data-work-id=58685986]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 58685986; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='58685986']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 58685986, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=58685986]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":58685986,"title":"Acute Respiratory Viral Infection Among Outpatient Healthcare Personnel","translated_title":"","metadata":{"abstract":"ABSTRACT Background: Respiratory disease is a common source of morbidity among healthcare personnel (HCP). However, the causes of acute respiratory illness (ARI) have not been well studied. We assessed the viral causes of respiratory illness among HCP enrolled in a cluster randomized clinical trial at 99 outpatient departments and emergency departments in 7 locations: Baltimore, MD; Denver, CO (3); Houston, TX; New York, NY; and Washington, DC. Methods: During 12 weeks of the 2011-2 (YR1) and 2012-3 (YR2) respiratory seasons, HCP were surveyed for signs and symptoms of ARI. Participants with symptoms were cultured and swabs frozen at -800C. Also, two random swabs were obtained during the intervention period. Samples were tested using a multiplex PCR for 13 viruses (PCR/ESI-MS, Abbott labs). Paired blood samples were obtained prior to the start of the intervention period and 2-weeks post-intervention, then tested for influenza antibodies (\u0026amp;gt;2-fold antibody increase). YR2 serum samples have not been tested. Results: Among 1686 participants (609 YR1; 1077 YR2), 4255 swabs were obtained. To date, 1579 swab samples have been tested (1370 YR1; 209 YR2): 544 symptomatic (335 YR1; 209 YR2) and 1035 asymptomatic. Influenza antibodies were tested in 1292 serum samples. Based on testing, 102 YR1 and 76 YR2 samples revealed the following viral isolates: influenza A (36), influenza B (8), parainfluenza (2), adenovirus (4), coronavirus (78), metapneumovirus (16), rhinovirus (17), and respiratory syncytial virus (RSV, 17) (Figure 1). Four cases of influenza A were identified by serological and viral testing (Figure 2). Conclusion: ARIs are common among HCP with 25% developing symptoms during the respiratory viral season. Among ARI in HCP, 27% had identifiable viral causes – most commonly coronavirus at 44%. This increased in YR2 with identification of rhinovirus. Most interesting is the significant number of asymptomatic HCP (19%) with identifiable virus. Some of these viruses cause significant morbidity to patients and transmission from HCP is critical to patient safety and that of the HCP themselves. *** Figure 1 Figure 2: Results show both serologic and nose/throat sample results. *Rhinovirus not tested. **Data available for 2 sites, asymptomatic data pending. ***NOTE: The data analysis is ongoing - more information will be available at the presentation.","publisher":"Oxford University Press (OUP)","publication_date":{"day":null,"month":null,"year":2015,"errors":{}},"publication_name":"Open Forum Infectious Diseases"},"translated_abstract":"ABSTRACT Background: Respiratory disease is a common source of morbidity among healthcare personnel (HCP). However, the causes of acute respiratory illness (ARI) have not been well studied. We assessed the viral causes of respiratory illness among HCP enrolled in a cluster randomized clinical trial at 99 outpatient departments and emergency departments in 7 locations: Baltimore, MD; Denver, CO (3); Houston, TX; New York, NY; and Washington, DC. Methods: During 12 weeks of the 2011-2 (YR1) and 2012-3 (YR2) respiratory seasons, HCP were surveyed for signs and symptoms of ARI. Participants with symptoms were cultured and swabs frozen at -800C. Also, two random swabs were obtained during the intervention period. Samples were tested using a multiplex PCR for 13 viruses (PCR/ESI-MS, Abbott labs). Paired blood samples were obtained prior to the start of the intervention period and 2-weeks post-intervention, then tested for influenza antibodies (\u0026amp;gt;2-fold antibody increase). YR2 serum samples have not been tested. Results: Among 1686 participants (609 YR1; 1077 YR2), 4255 swabs were obtained. To date, 1579 swab samples have been tested (1370 YR1; 209 YR2): 544 symptomatic (335 YR1; 209 YR2) and 1035 asymptomatic. Influenza antibodies were tested in 1292 serum samples. Based on testing, 102 YR1 and 76 YR2 samples revealed the following viral isolates: influenza A (36), influenza B (8), parainfluenza (2), adenovirus (4), coronavirus (78), metapneumovirus (16), rhinovirus (17), and respiratory syncytial virus (RSV, 17) (Figure 1). Four cases of influenza A were identified by serological and viral testing (Figure 2). Conclusion: ARIs are common among HCP with 25% developing symptoms during the respiratory viral season. Among ARI in HCP, 27% had identifiable viral causes – most commonly coronavirus at 44%. This increased in YR2 with identification of rhinovirus. Most interesting is the significant number of asymptomatic HCP (19%) with identifiable virus. Some of these viruses cause significant morbidity to patients and transmission from HCP is critical to patient safety and that of the HCP themselves. *** Figure 1 Figure 2: Results show both serologic and nose/throat sample results. *Rhinovirus not tested. **Data available for 2 sites, asymptomatic data pending. ***NOTE: The data analysis is ongoing - more information will be available at the presentation.","internal_url":"https://www.academia.edu/58685986/Acute_Respiratory_Viral_Infection_Among_Outpatient_Healthcare_Personnel","translated_internal_url":"","created_at":"2021-10-17T19:34:31.629-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":25381401,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Acute_Respiratory_Viral_Infection_Among_Outpatient_Healthcare_Personnel","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":25381401,"first_name":"Ranga","middle_initials":null,"last_name":"Sampath","page_name":"RangaSampath","domain_name":"independent","created_at":"2015-01-26T21:28:55.724-08:00","display_name":"Ranga Sampath","url":"https://independent.academia.edu/RangaSampath"},"attachments":[],"research_interests":[],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="58685985"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/58685985/Rapid_Molecular_Diagnostics_Antibiotic_Treatment_Decisions_and_Developing_Approaches_to_Inform_Empiric_Therapy_PRIMERS_I_and_II"><img alt="Research paper thumbnail of Rapid Molecular Diagnostics, Antibiotic Treatment Decisions, and Developing Approaches to Inform Empiric Therapy: PRIMERS I and II" class="work-thumbnail" src="https://attachments.academia-assets.com/72976694/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/58685985/Rapid_Molecular_Diagnostics_Antibiotic_Treatment_Decisions_and_Developing_Approaches_to_Inform_Empiric_Therapy_PRIMERS_I_and_II">Rapid Molecular Diagnostics, Antibiotic Treatment Decisions, and Developing Approaches to Inform Empiric Therapy: PRIMERS I and II</a></div><div class="wp-workCard_item"><span>Clinical infectious diseases : an official publication of the Infectious Diseases Society of America</span><span>, Jan 25, 2015</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated"> Rapid molecular diagnostic (RMD) platforms may lead to better antimicrobial stewardship. Our obj...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden"> Rapid molecular diagnostic (RMD) platforms may lead to better antimicrobial stewardship. Our objective was to develop an analytic strategy to enhance the interpretation of RMDs for the clinician. We compared the performance characteristics of four RMD platforms for detecting resistance against β-lactams in 72 highly drug-resistant isolates of Escherichia coli and Klebsiella pneumoniae (PRIMERS I). Subsequently, two platforms were utilized in a blinded study examining a collection of 196 isolates of E. coli and K. pneumoniae (PRIMERS II). We evaluated the genotypic results as predictors of resistance or susceptibility against a panel of common β-lactam antibiotics. Analytical strategies were designed to include graphical representations of platform performance: i) discrimination summary plots; and ii) determination of susceptibility and resistance predictive values that can be readily interpreted by skilled practitioners to help inform decision making. In PRIMERS I, the four RMD p...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="9afe79d2c83c6b891ed2c12e7de69155" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":72976694,"asset_id":58685985,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/72976694/download_file?st=MTczMjQyMjk1Myw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="58685985"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="58685985"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 58685985; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=58685985]").text(description); $(".js-view-count[data-work-id=58685985]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 58685985; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='58685985']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 58685985, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "9afe79d2c83c6b891ed2c12e7de69155" } } $('.js-work-strip[data-work-id=58685985]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":58685985,"title":"Rapid Molecular Diagnostics, Antibiotic Treatment Decisions, and Developing Approaches to Inform Empiric Therapy: PRIMERS I and II","translated_title":"","metadata":{"abstract":" Rapid molecular diagnostic (RMD) platforms may lead to better antimicrobial stewardship. Our objective was to develop an analytic strategy to enhance the interpretation of RMDs for the clinician. We compared the performance characteristics of four RMD platforms for detecting resistance against β-lactams in 72 highly drug-resistant isolates of Escherichia coli and Klebsiella pneumoniae (PRIMERS I). Subsequently, two platforms were utilized in a blinded study examining a collection of 196 isolates of E. coli and K. pneumoniae (PRIMERS II). We evaluated the genotypic results as predictors of resistance or susceptibility against a panel of common β-lactam antibiotics. Analytical strategies were designed to include graphical representations of platform performance: i) discrimination summary plots; and ii) determination of susceptibility and resistance predictive values that can be readily interpreted by skilled practitioners to help inform decision making. In PRIMERS I, the four RMD p...","publication_date":{"day":25,"month":1,"year":2015,"errors":{}},"publication_name":"Clinical infectious diseases : an official publication of the Infectious Diseases Society of America"},"translated_abstract":" Rapid molecular diagnostic (RMD) platforms may lead to better antimicrobial stewardship. Our objective was to develop an analytic strategy to enhance the interpretation of RMDs for the clinician. We compared the performance characteristics of four RMD platforms for detecting resistance against β-lactams in 72 highly drug-resistant isolates of Escherichia coli and Klebsiella pneumoniae (PRIMERS I). Subsequently, two platforms were utilized in a blinded study examining a collection of 196 isolates of E. coli and K. pneumoniae (PRIMERS II). We evaluated the genotypic results as predictors of resistance or susceptibility against a panel of common β-lactam antibiotics. Analytical strategies were designed to include graphical representations of platform performance: i) discrimination summary plots; and ii) determination of susceptibility and resistance predictive values that can be readily interpreted by skilled practitioners to help inform decision making. In PRIMERS I, the four RMD p...","internal_url":"https://www.academia.edu/58685985/Rapid_Molecular_Diagnostics_Antibiotic_Treatment_Decisions_and_Developing_Approaches_to_Inform_Empiric_Therapy_PRIMERS_I_and_II","translated_internal_url":"","created_at":"2021-10-17T19:34:31.511-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":25381401,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":72976694,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/72976694/thumbnails/1.jpg","file_name":"civ837.pdf","download_url":"https://www.academia.edu/attachments/72976694/download_file?st=MTczMjQyMjk1Myw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Rapid_Molecular_Diagnostics_Antibiotic_T.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/72976694/civ837-libre.pdf?1634525437=\u0026response-content-disposition=attachment%3B+filename%3DRapid_Molecular_Diagnostics_Antibiotic_T.pdf\u0026Expires=1732426553\u0026Signature=DqdfkECuRMHbXuROW0IBaNNm399hI7adhbJeV4K4PoeFxkf65zic3JGahHqGW-NMtcp3p4wDlqZczCKqU34Del2ZhU4a1yfPTjvF1GezDOFG0E0v4P9RDDiGgt6Qr0sKzVI2yVn2OZY5a~LwlcdxBi71lrwXWc6S-WJtjC7NXytWcwXBFoVOIdpHha5RNmjnFrnE0wPUGvSrqUMWJQ9kDN-DuBuxZ7LkbFkFw0o600M-f83OTKUhASD0lhFLJJSfEGq1DiKDCIBow0gY-kJSYq-K2ovbyzuCPnW6z2GdOkrxid3ITbxk~8~QLPyLPedP5sP3EU4hH7fWYOPLS8xmXw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Rapid_Molecular_Diagnostics_Antibiotic_Treatment_Decisions_and_Developing_Approaches_to_Inform_Empiric_Therapy_PRIMERS_I_and_II","translated_slug":"","page_count":9,"language":"en","content_type":"Work","owner":{"id":25381401,"first_name":"Ranga","middle_initials":null,"last_name":"Sampath","page_name":"RangaSampath","domain_name":"independent","created_at":"2015-01-26T21:28:55.724-08:00","display_name":"Ranga Sampath","url":"https://independent.academia.edu/RangaSampath"},"attachments":[{"id":72976694,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/72976694/thumbnails/1.jpg","file_name":"civ837.pdf","download_url":"https://www.academia.edu/attachments/72976694/download_file?st=MTczMjQyMjk1Myw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Rapid_Molecular_Diagnostics_Antibiotic_T.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/72976694/civ837-libre.pdf?1634525437=\u0026response-content-disposition=attachment%3B+filename%3DRapid_Molecular_Diagnostics_Antibiotic_T.pdf\u0026Expires=1732426553\u0026Signature=DqdfkECuRMHbXuROW0IBaNNm399hI7adhbJeV4K4PoeFxkf65zic3JGahHqGW-NMtcp3p4wDlqZczCKqU34Del2ZhU4a1yfPTjvF1GezDOFG0E0v4P9RDDiGgt6Qr0sKzVI2yVn2OZY5a~LwlcdxBi71lrwXWc6S-WJtjC7NXytWcwXBFoVOIdpHha5RNmjnFrnE0wPUGvSrqUMWJQ9kDN-DuBuxZ7LkbFkFw0o600M-f83OTKUhASD0lhFLJJSfEGq1DiKDCIBow0gY-kJSYq-K2ovbyzuCPnW6z2GdOkrxid3ITbxk~8~QLPyLPedP5sP3EU4hH7fWYOPLS8xmXw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences"},{"id":64568,"name":"Humans","url":"https://www.academia.edu/Documents/in/Humans"},{"id":83128,"name":"Escherichia coli","url":"https://www.academia.edu/Documents/in/Escherichia_coli"},{"id":335984,"name":"Anti-Bacterial Agents","url":"https://www.academia.edu/Documents/in/Anti-Bacterial_Agents"},{"id":413195,"name":"Time Factors","url":"https://www.academia.edu/Documents/in/Time_Factors"},{"id":561014,"name":"Microbial Sensitivity Tests","url":"https://www.academia.edu/Documents/in/Microbial_Sensitivity_Tests"},{"id":839246,"name":"Molecular Diagnostic Techniques","url":"https://www.academia.edu/Documents/in/Molecular_Diagnostic_Techniques"},{"id":1336838,"name":"Clinical Infectious Diseases","url":"https://www.academia.edu/Documents/in/Clinical_Infectious_Diseases"},{"id":2037499,"name":"Klebsiella pneumoniae","url":"https://www.academia.edu/Documents/in/Klebsiella_pneumoniae"},{"id":3643424,"name":"beta-Lactam Resistance","url":"https://www.academia.edu/Documents/in/beta-Lactam_Resistance"},{"id":3763225,"name":"Medical and Health Sciences","url":"https://www.academia.edu/Documents/in/Medical_and_Health_Sciences"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="58685984"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/58685984/A_Broad_Range_Polymerase_Chain_Reaction_Coupled_to_Electrospray_Ionization_Mass_Spectrometry_for_Detection_of_Biothreat_Agents_and_Common_Pathogens_in_Respiratory_Samples"><img alt="Research paper thumbnail of A Broad-Range Polymerase Chain Reaction Coupled to Electrospray Ionization Mass Spectrometry for Detection of Biothreat Agents and Common Pathogens in Respiratory Samples" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/58685984/A_Broad_Range_Polymerase_Chain_Reaction_Coupled_to_Electrospray_Ionization_Mass_Spectrometry_for_Detection_of_Biothreat_Agents_and_Common_Pathogens_in_Respiratory_Samples">A Broad-Range Polymerase Chain Reaction Coupled to Electrospray Ionization Mass Spectrometry for Detection of Biothreat Agents and Common Pathogens in Respiratory Samples</a></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Background: Biological threat (BT) agent infections (i.e. Anthrax, Plague, and Tularemia) often p...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Background: Biological threat (BT) agent infections (i.e. Anthrax, Plague, and Tularemia) often present with symptoms similar to other common respiratory infections (i.e. influenza like illness). New diagnostic platforms for respiratory pathogens must be capable of sensitive, specific, and rapid differentiation of BT organisms and common pathogens from normal flora. Base composition characterization by electrospray ionization mass spectrometry (ESI-MS) of nucleic acids amplified via RT-PCR from human respiratory samples allows for broad pathogen identification within eight hours after collection. In this study, we investigated the performance characteristics of RT-PCR/ESI-MS for distinguishing BT agents and common bacterial, fungal, and viral respiratory pathogens from normal flora in clinical samples from subjects with suspected respiratory infections. Methods: Bronchoalveolar lavages (BALs) and nasopharyngeal (NP) swab samples were collected from patients with acute respiratory in...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="58685984"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="58685984"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 58685984; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=58685984]").text(description); $(".js-view-count[data-work-id=58685984]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 58685984; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='58685984']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 58685984, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=58685984]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":58685984,"title":"A Broad-Range Polymerase Chain Reaction Coupled to Electrospray Ionization Mass Spectrometry for Detection of Biothreat Agents and Common Pathogens in Respiratory Samples","translated_title":"","metadata":{"abstract":"Background: Biological threat (BT) agent infections (i.e. Anthrax, Plague, and Tularemia) often present with symptoms similar to other common respiratory infections (i.e. influenza like illness). New diagnostic platforms for respiratory pathogens must be capable of sensitive, specific, and rapid differentiation of BT organisms and common pathogens from normal flora. Base composition characterization by electrospray ionization mass spectrometry (ESI-MS) of nucleic acids amplified via RT-PCR from human respiratory samples allows for broad pathogen identification within eight hours after collection. In this study, we investigated the performance characteristics of RT-PCR/ESI-MS for distinguishing BT agents and common bacterial, fungal, and viral respiratory pathogens from normal flora in clinical samples from subjects with suspected respiratory infections. Methods: Bronchoalveolar lavages (BALs) and nasopharyngeal (NP) swab samples were collected from patients with acute respiratory in..."},"translated_abstract":"Background: Biological threat (BT) agent infections (i.e. Anthrax, Plague, and Tularemia) often present with symptoms similar to other common respiratory infections (i.e. influenza like illness). New diagnostic platforms for respiratory pathogens must be capable of sensitive, specific, and rapid differentiation of BT organisms and common pathogens from normal flora. Base composition characterization by electrospray ionization mass spectrometry (ESI-MS) of nucleic acids amplified via RT-PCR from human respiratory samples allows for broad pathogen identification within eight hours after collection. In this study, we investigated the performance characteristics of RT-PCR/ESI-MS for distinguishing BT agents and common bacterial, fungal, and viral respiratory pathogens from normal flora in clinical samples from subjects with suspected respiratory infections. Methods: Bronchoalveolar lavages (BALs) and nasopharyngeal (NP) swab samples were collected from patients with acute respiratory in...","internal_url":"https://www.academia.edu/58685984/A_Broad_Range_Polymerase_Chain_Reaction_Coupled_to_Electrospray_Ionization_Mass_Spectrometry_for_Detection_of_Biothreat_Agents_and_Common_Pathogens_in_Respiratory_Samples","translated_internal_url":"","created_at":"2021-10-17T19:34:31.410-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":25381401,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"A_Broad_Range_Polymerase_Chain_Reaction_Coupled_to_Electrospray_Ionization_Mass_Spectrometry_for_Detection_of_Biothreat_Agents_and_Common_Pathogens_in_Respiratory_Samples","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":25381401,"first_name":"Ranga","middle_initials":null,"last_name":"Sampath","page_name":"RangaSampath","domain_name":"independent","created_at":"2015-01-26T21:28:55.724-08:00","display_name":"Ranga Sampath","url":"https://independent.academia.edu/RangaSampath"},"attachments":[],"research_interests":[],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="58685982"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/58685982/Genotypic_Variation_and_Mixtures_of_Lyme_Borrelia_in_Ixodes_Ticks_from_North_America_and_Europe"><img alt="Research paper thumbnail of Genotypic Variation and Mixtures of Lyme Borrelia in Ixodes Ticks from North America and Europe" class="work-thumbnail" src="https://attachments.academia-assets.com/72976738/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/58685982/Genotypic_Variation_and_Mixtures_of_Lyme_Borrelia_in_Ixodes_Ticks_from_North_America_and_Europe">Genotypic Variation and Mixtures of Lyme Borrelia in Ixodes Ticks from North America and Europe</a></div><div class="wp-workCard_item"><span>PLoS ONE</span><span>, 2010</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="b76e693d0e151ead983e87e8cee4359b" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":72976738,"asset_id":58685982,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/72976738/download_file?st=MTczMjQyMjk1Myw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="58685982"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="58685982"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 58685982; 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Studies have shown the genotype of Borrelia burgdorferi sensu stricto (s.s.) or the species of B. burgdorferi sensu lato (s.l.) affects the ability of the bacteria to cause local or disseminated infection in humans. Methodology/Principal Findings: We used a multilocus PCR electrospray mass spectrometry assay to determine the species and genotype Borrelia from ticks collected in New York, Connecticut, Indiana, Southern Germany, and California and characterized isolates from parts of the United States and Europe. These analyses identified 53 distinct genotypes of B. burgdorferi sensu stricto with higher resolution than ospC typing. Genotypes of other members of the B. burgdorferi sensu lato complex were also identified and genotyped including B. afzelii, B. garinii, B. lusitaniae, B. spielmanii, and B. valaisiana. While each site in North America had genotypes unique to that location, we found genotypes shared between individual regions and two genotypes found across the United States. Significant B. burgdorferi s.s. genotypic diversity was observed between North America and Europe: only 6.6% of US genotypes (3 of 45) were found in Europe and 27% of the European genotypes (3 of 11) were observed in the US. Interestingly, 39% of adult Ixodes scapularis ticks from North America were infected with more than one genotype of B. burgdorferi s.s. and 22.2% of Ixodes ricinus ticks from Germany were infected with more than one genotype of B. burgdorferi s.l. Conclusions/Significance: The presence of multiple Borrelia genotypes in ticks increases the probability that a person will be infected with more than one genotype of B. burgdorferi, potentially increasing the risks of disseminated Lyme disease. Our study indicates that the genotypic diversity of Borrelia in ticks in both North America and Europe is higher then previously reported and can have potential clinical consequences.","publication_date":{"day":null,"month":null,"year":2010,"errors":{}},"publication_name":"PLoS ONE","grobid_abstract_attachment_id":72976738},"translated_abstract":null,"internal_url":"https://www.academia.edu/58685982/Genotypic_Variation_and_Mixtures_of_Lyme_Borrelia_in_Ixodes_Ticks_from_North_America_and_Europe","translated_internal_url":"","created_at":"2021-10-17T19:34:31.303-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":25381401,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":72976738,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/72976738/thumbnails/1.jpg","file_name":"Genotypic_variation_and_mixtures_of_Lyme20211017-13084-1y304xx.pdf","download_url":"https://www.academia.edu/attachments/72976738/download_file?st=MTczMjQyMjk1Myw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Genotypic_Variation_and_Mixtures_of_Lyme.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/72976738/Genotypic_variation_and_mixtures_of_Lyme20211017-13084-1y304xx.pdf?1634524576=\u0026response-content-disposition=attachment%3B+filename%3DGenotypic_Variation_and_Mixtures_of_Lyme.pdf\u0026Expires=1732426553\u0026Signature=Tf6fzoIb6J~0d64pXlrnRbYN3T1rujhPOZXySutTwlKItw1fkWsFlxcU1q0Bqq-YZ3EHg4G6sRzYAFvbIe1LNIbcRn4G75fgIcOrzhXNbyr04K-JLM2DZxnl-U0io83B~sWGNZ1Bw6PUz3VIeIGXcBUQqu-TyPBnPVR8bIU1NpySttYcPchbImOfPvRRzqdYpjLc-35zzr9fgWL6rm38mbXMSZHxiw40tNRSmx-mq7klnom~jClzw-yJ-FqBWtKBirM0Kcd~wWtQm6~EWU2S1CLkpo8zk8IxwYv2O0yljuHO4PaRaRue6DCX27si2YPBXbl4ZB7Yg82jlIK89mNW~g__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Genotypic_Variation_and_Mixtures_of_Lyme_Borrelia_in_Ixodes_Ticks_from_North_America_and_Europe","translated_slug":"","page_count":9,"language":"en","content_type":"Work","owner":{"id":25381401,"first_name":"Ranga","middle_initials":null,"last_name":"Sampath","page_name":"RangaSampath","domain_name":"independent","created_at":"2015-01-26T21:28:55.724-08:00","display_name":"Ranga 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Spectrometry","url":"https://www.academia.edu/Documents/in/Mass_Spectrometry"},{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":28235,"name":"Multidisciplinary","url":"https://www.academia.edu/Documents/in/Multidisciplinary"},{"id":53198,"name":"Lyme disease","url":"https://www.academia.edu/Documents/in/Lyme_disease"},{"id":64568,"name":"Humans","url":"https://www.academia.edu/Documents/in/Humans"},{"id":75826,"name":"Europe","url":"https://www.academia.edu/Documents/in/Europe"},{"id":99234,"name":"Animals","url":"https://www.academia.edu/Documents/in/Animals"},{"id":128172,"name":"Borrelia burgdorferi","url":"https://www.academia.edu/Documents/in/Borrelia_burgdorferi"},{"id":361848,"name":"Ixodes 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data-work-id="58685981"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/58685981/Rev_response_elements_RRE_in_lentiviruses_An_RNAMotif_algorithm_based_strategy_for_RRE_prediction"><img alt="Research paper thumbnail of Rev response elements (RRE) in lentiviruses: An RNAMotif algorithm-based strategy for RRE prediction" class="work-thumbnail" src="https://attachments.academia-assets.com/72976753/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/58685981/Rev_response_elements_RRE_in_lentiviruses_An_RNAMotif_algorithm_based_strategy_for_RRE_prediction">Rev response elements (RRE) in lentiviruses: An RNAMotif algorithm-based strategy for RRE prediction</a></div><div class="wp-workCard_item"><span>Medicinal Research Reviews</span><span>, 2002</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="0e075c2961f18347f3a95eb80898a613" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":72976753,"asset_id":58685981,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/72976753/download_file?st=MTczMjQyMjk1Myw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="58685981"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span 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WowProfile.WorkStripView({ el: this, workJSON: {"id":58685981,"title":"Rev response elements (RRE) in lentiviruses: An RNAMotif algorithm-based strategy for RRE prediction","translated_title":"","metadata":{"publisher":"Wiley","grobid_abstract":"Lentiviruses (a sub-family of the retroviridae family) include primate and non-primate viruses associated with chronic diseases of the immune system and the central nervous system. All lentiviruses encode a regulatory protein Rev that is essential for post-transcriptional transport of the unspliced and incompletely spliced viral mRNAs from nuclei to cytoplasm. The Rev protein acts via binding to an RNA structural element known as the Rev responsive element (RRE). The RRE location and structure and the mechanism of the Rev-RRE interaction in primate and non-primate lentiviruses have been analyzed and compared. Based on structural data available for RRE of HIV-1, a two step computational strategy for prediction of putative RRE regions in lentivirus genomes has been developed. First, the RNAMotif algorithm was used to search genomic sequence for highly structured regions (HSR). Then the program RNAstructure, version 3.6 was used to calculate the structure and thermodynamic stability of the region of $ 350 nucleotides encompassing the HSR. Our strategy correctly predicted the locations of all previously reported lentivirus RREs. We were able also to predict the locations and structures of potential RREs in four additional lentiviruses.","publication_date":{"day":null,"month":null,"year":2002,"errors":{}},"publication_name":"Medicinal Research Reviews","grobid_abstract_attachment_id":72976753},"translated_abstract":null,"internal_url":"https://www.academia.edu/58685981/Rev_response_elements_RRE_in_lentiviruses_An_RNAMotif_algorithm_based_strategy_for_RRE_prediction","translated_internal_url":"","created_at":"2021-10-17T19:34:31.197-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":25381401,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":72976753,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/72976753/thumbnails/1.jpg","file_name":"med.1002720211017-5334-19owgn5.pdf","download_url":"https://www.academia.edu/attachments/72976753/download_file?st=MTczMjQyMjk1Myw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Rev_response_elements_RRE_in_lentiviruse.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/72976753/med.1002720211017-5334-19owgn5-libre.pdf?1634525432=\u0026response-content-disposition=attachment%3B+filename%3DRev_response_elements_RRE_in_lentiviruse.pdf\u0026Expires=1732426553\u0026Signature=dStq1fOnDQK6kRcPMH~JwlKnmtN0b~6nEtrRkayR3lUQM1Ga5cq7RVKQIOZebisvCVE~b2OKHNmJ-c1jJqE-jcpA07s1ucy7Y6aXIRHsTXZnJxP3shO15T9dWySwYwvv-wPkJHQ7bM6Ts~XTTt6J3l1NwIlMWOBzufIlaVd5vGhfY74tuTzKMhQE7EU9RCT7Os0OR0ClN2wg7osYS3YS-VY2N6m46Xhy5k~aK6wpgDPkRj-~CHSYLZ4t8izH~Dq1xUfufTz-6HA49Tam1KVvAGKHYdkY~EzX9kJJFNud-PBTA9yF-YzmLgulPV-IyvnoV9~67gWvlpDEz2b5y5zPCA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Rev_response_elements_RRE_in_lentiviruses_An_RNAMotif_algorithm_based_strategy_for_RRE_prediction","translated_slug":"","page_count":20,"language":"en","content_type":"Work","owner":{"id":25381401,"first_name":"Ranga","middle_initials":null,"last_name":"Sampath","page_name":"RangaSampath","domain_name":"independent","created_at":"2015-01-26T21:28:55.724-08:00","display_name":"Ranga Sampath","url":"https://independent.academia.edu/RangaSampath"},"attachments":[{"id":72976753,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/72976753/thumbnails/1.jpg","file_name":"med.1002720211017-5334-19owgn5.pdf","download_url":"https://www.academia.edu/attachments/72976753/download_file?st=MTczMjQyMjk1Myw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Rev_response_elements_RRE_in_lentiviruse.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/72976753/med.1002720211017-5334-19owgn5-libre.pdf?1634525432=\u0026response-content-disposition=attachment%3B+filename%3DRev_response_elements_RRE_in_lentiviruse.pdf\u0026Expires=1732426553\u0026Signature=dStq1fOnDQK6kRcPMH~JwlKnmtN0b~6nEtrRkayR3lUQM1Ga5cq7RVKQIOZebisvCVE~b2OKHNmJ-c1jJqE-jcpA07s1ucy7Y6aXIRHsTXZnJxP3shO15T9dWySwYwvv-wPkJHQ7bM6Ts~XTTt6J3l1NwIlMWOBzufIlaVd5vGhfY74tuTzKMhQE7EU9RCT7Os0OR0ClN2wg7osYS3YS-VY2N6m46Xhy5k~aK6wpgDPkRj-~CHSYLZ4t8izH~Dq1xUfufTz-6HA49Tam1KVvAGKHYdkY~EzX9kJJFNud-PBTA9yF-YzmLgulPV-IyvnoV9~67gWvlpDEz2b5y5zPCA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":428,"name":"Algorithms","url":"https://www.academia.edu/Documents/in/Algorithms"},{"id":522,"name":"Thermodynamics","url":"https://www.academia.edu/Documents/in/Thermodynamics"},{"id":4233,"name":"Computational Biology","url":"https://www.academia.edu/Documents/in/Computational_Biology"},{"id":39978,"name":"HIV","url":"https://www.academia.edu/Documents/in/HIV"},{"id":52714,"name":"Primates","url":"https://www.academia.edu/Documents/in/Primates"},{"id":64568,"name":"Humans","url":"https://www.academia.edu/Documents/in/Humans"},{"id":99234,"name":"Animals","url":"https://www.academia.edu/Documents/in/Animals"},{"id":140613,"name":"Lentivirus","url":"https://www.academia.edu/Documents/in/Lentivirus"},{"id":1643624,"name":"Response Elements","url":"https://www.academia.edu/Documents/in/Response_Elements"},{"id":3789884,"name":"Pharmacology and pharmaceutical sciences","url":"https://www.academia.edu/Documents/in/Pharmacology_and_pharmaceutical_sciences"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="58685980"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/58685980/Detection_of_heartworm_infection_in_dogs_via_PCR_amplification_and_electrospray_ionization_mass_spectrometry_of_nucleic_acid_extracts_from_whole_blood_samples"><img alt="Research paper thumbnail of Detection of heartworm infection in dogs via PCR amplification and electrospray ionization mass spectrometry of nucleic acid extracts from whole blood samples" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/58685980/Detection_of_heartworm_infection_in_dogs_via_PCR_amplification_and_electrospray_ionization_mass_spectrometry_of_nucleic_acid_extracts_from_whole_blood_samples">Detection of heartworm infection in dogs via PCR amplification and electrospray ionization mass spectrometry of nucleic acid extracts from whole blood samples</a></div><div class="wp-workCard_item"><span>American Journal of Veterinary Research</span><span>, 2012</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">To develop and evaluate a rapid and accurate assay involving PCR amplification and electrospray i...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">To develop and evaluate a rapid and accurate assay involving PCR amplification and electrospray ionization mass spectrometry of nucleic acid extracts from whole blood samples for the detection of Dirofilaria immitis infection in dogs. Whole blood nucleic acid extracts from 29 dogs experimentally infected with D immitis (and in which circulating D immitis antigen was detected) and 10 uninfected dogs. 16 of the 29 whole blood samples from infected dogs were examined at the time of collection for circulating microfilaria. Nucleic acids were extracted from all whole blood specimens and underwent PCR amplification with 12 PCR primer pairs designed to detect a wide range of pathogens (including the Wolbachia endosymbiont of D immitis) and electrospray ionization mass spectrometry. On the basis of assay results, heartworm infection was detected in 13 of 13 antigen-positive dogs of unknown microfilaria status, 11 of 11 antigen-positive dogs with circulating microfilaria, 0 of 3 antigen-positive dogs tested at 3 months after larval infection, 0 of 2 antigen-positive dogs with occult infections, and 0 of 10 uninfected dogs. With the assay under investigation, it was possible to identify D immitis infection in dogs with circulating microfilaria via detection of the obligate Wolbachia endosymbiont of D immitis. It was not possible to identify dogs with occult infections, which suggested that circulating microfilaria must be present to detect infection with this assay, although further studies would be required to verify that finding.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="58685980"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="58685980"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 58685980; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=58685980]").text(description); $(".js-view-count[data-work-id=58685980]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 58685980; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='58685980']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 58685980, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=58685980]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":58685980,"title":"Detection of heartworm infection in dogs via PCR amplification and electrospray ionization mass spectrometry of nucleic acid extracts from whole blood samples","translated_title":"","metadata":{"abstract":"To develop and evaluate a rapid and accurate assay involving PCR amplification and electrospray ionization mass spectrometry of nucleic acid extracts from whole blood samples for the detection of Dirofilaria immitis infection in dogs. Whole blood nucleic acid extracts from 29 dogs experimentally infected with D immitis (and in which circulating D immitis antigen was detected) and 10 uninfected dogs. 16 of the 29 whole blood samples from infected dogs were examined at the time of collection for circulating microfilaria. Nucleic acids were extracted from all whole blood specimens and underwent PCR amplification with 12 PCR primer pairs designed to detect a wide range of pathogens (including the Wolbachia endosymbiont of D immitis) and electrospray ionization mass spectrometry. On the basis of assay results, heartworm infection was detected in 13 of 13 antigen-positive dogs of unknown microfilaria status, 11 of 11 antigen-positive dogs with circulating microfilaria, 0 of 3 antigen-positive dogs tested at 3 months after larval infection, 0 of 2 antigen-positive dogs with occult infections, and 0 of 10 uninfected dogs. With the assay under investigation, it was possible to identify D immitis infection in dogs with circulating microfilaria via detection of the obligate Wolbachia endosymbiont of D immitis. It was not possible to identify dogs with occult infections, which suggested that circulating microfilaria must be present to detect infection with this assay, although further studies would be required to verify that finding.","publisher":"American Veterinary Medical Association (AVMA)","publication_date":{"day":null,"month":null,"year":2012,"errors":{}},"publication_name":"American Journal of Veterinary Research"},"translated_abstract":"To develop and evaluate a rapid and accurate assay involving PCR amplification and electrospray ionization mass spectrometry of nucleic acid extracts from whole blood samples for the detection of Dirofilaria immitis infection in dogs. Whole blood nucleic acid extracts from 29 dogs experimentally infected with D immitis (and in which circulating D immitis antigen was detected) and 10 uninfected dogs. 16 of the 29 whole blood samples from infected dogs were examined at the time of collection for circulating microfilaria. Nucleic acids were extracted from all whole blood specimens and underwent PCR amplification with 12 PCR primer pairs designed to detect a wide range of pathogens (including the Wolbachia endosymbiont of D immitis) and electrospray ionization mass spectrometry. On the basis of assay results, heartworm infection was detected in 13 of 13 antigen-positive dogs of unknown microfilaria status, 11 of 11 antigen-positive dogs with circulating microfilaria, 0 of 3 antigen-positive dogs tested at 3 months after larval infection, 0 of 2 antigen-positive dogs with occult infections, and 0 of 10 uninfected dogs. With the assay under investigation, it was possible to identify D immitis infection in dogs with circulating microfilaria via detection of the obligate Wolbachia endosymbiont of D immitis. It was not possible to identify dogs with occult infections, which suggested that circulating microfilaria must be present to detect infection with this assay, although further studies would be required to verify that finding.","internal_url":"https://www.academia.edu/58685980/Detection_of_heartworm_infection_in_dogs_via_PCR_amplification_and_electrospray_ionization_mass_spectrometry_of_nucleic_acid_extracts_from_whole_blood_samples","translated_internal_url":"","created_at":"2021-10-17T19:34:31.090-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":25381401,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Detection_of_heartworm_infection_in_dogs_via_PCR_amplification_and_electrospray_ionization_mass_spectrometry_of_nucleic_acid_extracts_from_whole_blood_samples","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":25381401,"first_name":"Ranga","middle_initials":null,"last_name":"Sampath","page_name":"RangaSampath","domain_name":"independent","created_at":"2015-01-26T21:28:55.724-08:00","display_name":"Ranga Sampath","url":"https://independent.academia.edu/RangaSampath"},"attachments":[],"research_interests":[{"id":5769,"name":"Mass Spectrometry","url":"https://www.academia.edu/Documents/in/Mass_Spectrometry"},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences"},{"id":52438,"name":"Dogs","url":"https://www.academia.edu/Documents/in/Dogs"},{"id":99234,"name":"Animals","url":"https://www.academia.edu/Documents/in/Animals"},{"id":118339,"name":"Polymerase Chain Reaction","url":"https://www.academia.edu/Documents/in/Polymerase_Chain_Reaction"},{"id":178906,"name":"Nucleic Acids","url":"https://www.academia.edu/Documents/in/Nucleic_Acids"},{"id":809882,"name":"Base Sequence","url":"https://www.academia.edu/Documents/in/Base_Sequence"},{"id":973347,"name":"Ribosomal DNA","url":"https://www.academia.edu/Documents/in/Ribosomal_DNA"},{"id":2467566,"name":"Molecular Sequence Data","url":"https://www.academia.edu/Documents/in/Molecular_Sequence_Data"},{"id":3067128,"name":"reverse transcriptase polymerase chain reaction","url":"https://www.academia.edu/Documents/in/reverse_transcriptase_polymerase_chain_reaction"},{"id":3253374,"name":"Dirofilariasis","url":"https://www.academia.edu/Documents/in/Dirofilariasis"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="58685915"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/58685915/Usefulness_of_multilocus_polymerase_chain_reaction_followed_by_electrospray_ionization_mass_spectrometry_to_identify_a_diverse_panel_of_bacterial_isolates"><img alt="Research paper thumbnail of Usefulness of multilocus polymerase chain reaction followed by electrospray ionization mass spectrometry to identify a diverse panel of bacterial isolates" class="work-thumbnail" src="https://attachments.academia-assets.com/72976716/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/58685915/Usefulness_of_multilocus_polymerase_chain_reaction_followed_by_electrospray_ionization_mass_spectrometry_to_identify_a_diverse_panel_of_bacterial_isolates">Usefulness of multilocus polymerase chain reaction followed by electrospray ionization mass spectrometry to identify a diverse panel of bacterial isolates</a></div><div class="wp-workCard_item"><span>Diagnostic microbiology and infectious disease</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Polymerase chain reaction electrospray ionization mass spectrometry (PCR/ESI-MS) was tested for i...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Polymerase chain reaction electrospray ionization mass spectrometry (PCR/ESI-MS) was tested for its ability to accurately identify a blinded panel of 156 diverse bacterial isolates, mostly human and/or animal pathogens. Here, 142/156 (91%) isolates were correctly identified to the genus level and 115/156 (74%) were correctly identified to the species level. Only 9% were misidentified. This study shows that multilocus PCR/ESI-MS has the potential to be a useful technique for identifying a broad range of bacteria.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="e66f1738f5bb2068c2cfb1a44acff25e" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":72976716,"asset_id":58685915,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/72976716/download_file?st=MTczMjQyMjk1Myw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="58685915"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="58685915"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 58685915; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=58685915]").text(description); $(".js-view-count[data-work-id=58685915]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 58685915; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='58685915']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 58685915, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "e66f1738f5bb2068c2cfb1a44acff25e" } } $('.js-work-strip[data-work-id=58685915]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":58685915,"title":"Usefulness of multilocus polymerase chain reaction followed by electrospray ionization mass spectrometry to identify a diverse panel of bacterial isolates","translated_title":"","metadata":{"abstract":"Polymerase chain reaction electrospray ionization mass spectrometry (PCR/ESI-MS) was tested for its ability to accurately identify a blinded panel of 156 diverse bacterial isolates, mostly human and/or animal pathogens. Here, 142/156 (91%) isolates were correctly identified to the genus level and 115/156 (74%) were correctly identified to the species level. Only 9% were misidentified. This study shows that multilocus PCR/ESI-MS has the potential to be a useful technique for identifying a broad range of bacteria.","publication_name":"Diagnostic microbiology and infectious disease"},"translated_abstract":"Polymerase chain reaction electrospray ionization mass spectrometry (PCR/ESI-MS) was tested for its ability to accurately identify a blinded panel of 156 diverse bacterial isolates, mostly human and/or animal pathogens. Here, 142/156 (91%) isolates were correctly identified to the genus level and 115/156 (74%) were correctly identified to the species level. Only 9% were misidentified. 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href="https://www.academia.edu/95254919/S08_1_Diagnostic_Infectious_diseases_self_testing_and_self_sampling_outside_clinics_A_global_systematic_review">S08.1 Diagnostic Infectious diseases self-testing and self-sampling outside clinics: A global systematic review</a></div><div class="wp-workCard_item"><span>Symposium presentations</span><span>, 2021</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="5998c46e1e445d4699b17ed7c62c4f76" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":97486986,"asset_id":95254919,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/97486986/download_file?st=MTczMjQyMjk1Myw4LjIyMi4yMDguMTQ2&st=MTczMjQyMjk1Myw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner 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Positive aspects of care include support and the provision of appropriate services from community led and implemented interventions. Recommendations are made in relation to reactions to an HIV positive diagnosis, to support disclosure, address structural impediments in and outside of the health facilities. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="70138687"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/70138687/Genotypic_Variation_and_Mixtures_of_Lyme_Borrelia_in_Ixodes_Ticks_from_North_America_and_Europe"><img alt="Research paper thumbnail of Genotypic Variation and Mixtures of Lyme Borrelia in Ixodes Ticks from North America and Europe" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/70138687/Genotypic_Variation_and_Mixtures_of_Lyme_Borrelia_in_Ixodes_Ticks_from_North_America_and_Europe">Genotypic Variation and Mixtures of Lyme Borrelia in Ixodes Ticks from North America and Europe</a></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Background: Lyme disease, caused by various species of Borrelia, is transmitted by Ixodes ticks i...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Background: Lyme disease, caused by various species of Borrelia, is transmitted by Ixodes ticks in North America and Europe. Studies have shown the genotype of Borrelia burgdorferi sensu stricto (s.s.) or the species of B. burgdorferi sensu lato (s.l.) affects the ability of the bacteria to cause local or disseminated infection in humans. Methodology/Principal Findings: We used a multilocus PCR electrospray mass spectrometry assay to determine the species and genotype Borrelia from ticks collected in New York, Connecticut, Indiana, Southern Germany, and California and characterized isolates from parts of the United States and Europe. These analyses identified 53 distinct genotypes of B. burgdorferi sensu stricto with higher resolution than ospC typing. Genotypes of other members of the B. burgdorferi sensu lato complex were also identified and genotyped including B. afzelii, B. garinii, B. lusitaniae, B. spielmanii, and B. valaisiana. While each site in North America had genotypes u...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="70138687"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="70138687"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 70138687; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=70138687]").text(description); $(".js-view-count[data-work-id=70138687]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 70138687; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='70138687']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 70138687, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=70138687]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":70138687,"title":"Genotypic Variation and Mixtures of Lyme Borrelia in Ixodes Ticks from North America and Europe","translated_title":"","metadata":{"abstract":"Background: Lyme disease, caused by various species of Borrelia, is transmitted by Ixodes ticks in North America and Europe. Studies have shown the genotype of Borrelia burgdorferi sensu stricto (s.s.) or the species of B. burgdorferi sensu lato (s.l.) affects the ability of the bacteria to cause local or disseminated infection in humans. Methodology/Principal Findings: We used a multilocus PCR electrospray mass spectrometry assay to determine the species and genotype Borrelia from ticks collected in New York, Connecticut, Indiana, Southern Germany, and California and characterized isolates from parts of the United States and Europe. These analyses identified 53 distinct genotypes of B. burgdorferi sensu stricto with higher resolution than ospC typing. Genotypes of other members of the B. burgdorferi sensu lato complex were also identified and genotyped including B. afzelii, B. garinii, B. lusitaniae, B. spielmanii, and B. valaisiana. While each site in North America had genotypes u...","publication_date":{"day":null,"month":null,"year":2013,"errors":{}}},"translated_abstract":"Background: Lyme disease, caused by various species of Borrelia, is transmitted by Ixodes ticks in North America and Europe. Studies have shown the genotype of Borrelia burgdorferi sensu stricto (s.s.) or the species of B. burgdorferi sensu lato (s.l.) affects the ability of the bacteria to cause local or disseminated infection in humans. Methodology/Principal Findings: We used a multilocus PCR electrospray mass spectrometry assay to determine the species and genotype Borrelia from ticks collected in New York, Connecticut, Indiana, Southern Germany, and California and characterized isolates from parts of the United States and Europe. These analyses identified 53 distinct genotypes of B. burgdorferi sensu stricto with higher resolution than ospC typing. Genotypes of other members of the B. burgdorferi sensu lato complex were also identified and genotyped including B. afzelii, B. garinii, B. lusitaniae, B. spielmanii, and B. valaisiana. While each site in North America had genotypes u...","internal_url":"https://www.academia.edu/70138687/Genotypic_Variation_and_Mixtures_of_Lyme_Borrelia_in_Ixodes_Ticks_from_North_America_and_Europe","translated_internal_url":"","created_at":"2022-01-31T04:22:39.339-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":25381401,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Genotypic_Variation_and_Mixtures_of_Lyme_Borrelia_in_Ixodes_Ticks_from_North_America_and_Europe","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":25381401,"first_name":"Ranga","middle_initials":null,"last_name":"Sampath","page_name":"RangaSampath","domain_name":"independent","created_at":"2015-01-26T21:28:55.724-08:00","display_name":"Ranga Sampath","url":"https://independent.academia.edu/RangaSampath"},"attachments":[],"research_interests":[{"id":48,"name":"Engineering","url":"https://www.academia.edu/Documents/in/Engineering"},{"id":261,"name":"Geography","url":"https://www.academia.edu/Documents/in/Geography"},{"id":523,"name":"Chemistry","url":"https://www.academia.edu/Documents/in/Chemistry"},{"id":5769,"name":"Mass Spectrometry","url":"https://www.academia.edu/Documents/in/Mass_Spectrometry"},{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":28235,"name":"Multidisciplinary","url":"https://www.academia.edu/Documents/in/Multidisciplinary"},{"id":53198,"name":"Lyme disease","url":"https://www.academia.edu/Documents/in/Lyme_disease"},{"id":64568,"name":"Humans","url":"https://www.academia.edu/Documents/in/Humans"},{"id":75826,"name":"Europe","url":"https://www.academia.edu/Documents/in/Europe"},{"id":99234,"name":"Animals","url":"https://www.academia.edu/Documents/in/Animals"},{"id":128172,"name":"Borrelia burgdorferi","url":"https://www.academia.edu/Documents/in/Borrelia_burgdorferi"},{"id":361848,"name":"Ixodes ricinus","url":"https://www.academia.edu/Documents/in/Ixodes_ricinus"},{"id":372410,"name":"Genotype","url":"https://www.academia.edu/Documents/in/Genotype"},{"id":577933,"name":"Genetic variation","url":"https://www.academia.edu/Documents/in/Genetic_variation"},{"id":756265,"name":"Borrelia","url":"https://www.academia.edu/Documents/in/Borrelia"},{"id":897281,"name":"Ixodes scapularis","url":"https://www.academia.edu/Documents/in/Ixodes_scapularis"},{"id":2457889,"name":"Genotypic diversity","url":"https://www.academia.edu/Documents/in/Genotypic_diversity"},{"id":2693601,"name":"Genotypic variation","url":"https://www.academia.edu/Documents/in/Genotypic_variation"},{"id":3114383,"name":"Ixodes","url":"https://www.academia.edu/Documents/in/Ixodes"}],"urls":[{"id":17157860,"url":"http://citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.354.3158\u0026rep=rep1\u0026type=pdf"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="70138686"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/70138686/Rapid_Detection_and_Characterization_of_the_NAP_1_Epidemic_Strain_of_Clostridium_difficile_Using_PCR_ESI_MS"><img alt="Research paper thumbnail of Rapid Detection and Characterization of the NAP-1 Epidemic Strain of Clostridium difficile Using PCR/ESI-MS" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/70138686/Rapid_Detection_and_Characterization_of_the_NAP_1_Epidemic_Strain_of_Clostridium_difficile_Using_PCR_ESI_MS">Rapid Detection and Characterization of the NAP-1 Epidemic Strain of Clostridium difficile Using PCR/ESI-MS</a></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">ABSTRACT Background: Increasing rate and severity of C. difficile -associated disease (CDAD) have...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">ABSTRACT Background: Increasing rate and severity of C. difficile -associated disease (CDAD) have been associated with the emergence of the NAP-1 strain of C. difficile. Enhanced virulence is associated with deletions within the pathogenicity locus gene, tcdC. We hypothesize that previously known and as yet unknown mutations in the tcd gene cluster may be important for fully understanding CDAD. Methods: We have developed a PCR Electrospray Ionization Mass Spectrometry (PCR/ESI-MS) based method to rapidly identify C. difficile and detect multiple mutations in the tcdC locus, including the previously described 18 base pair deletion and single nucleotide deletion associated with NAP-1 strains, while simultaneously detecting tcdA, tcdB, cdtA and cdtB. Results: PCR/ESI-MS was performed on 65 archived C. difficile clinical isolates that had been collected during previous studies at JHH. 28 of the 65 isolates showed distinct NAP-1 like deletions in the tcdC loci. All 28 had both the 18 bp deletion and an additional single base pair deletion in the promoter region characteristic of fully virulent strains. Two distinct genotypes were observed in the tcdB locus- all 28 virulent strains had a C to T SNP that has not been previously reported. Overall, of the 65 isolates tested, 54 were tcdA+/B+, with 33 showing the tcdB SNP. 4 of the 5 remaining isolates with this SNP had the previously described 36 bp deletion with unknown phenotype in the tcdC locus. Conclusion: The PCR/ESI-MS technology is a high throughput assay system useful for rapid detection and characterization of CDAD. It is capable of simultaneous identification of C. difficile and detection of several important genotypic markers that might be associated with virulence. In addition to detecting all previously reported mutations, it has the potential to uncover additional mutations or correlations that might help understand the virulence properties of this microbe.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="70138686"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="70138686"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 70138686; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=70138686]").text(description); $(".js-view-count[data-work-id=70138686]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 70138686; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='70138686']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 70138686, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=70138686]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":70138686,"title":"Rapid Detection and Characterization of the NAP-1 Epidemic Strain of Clostridium difficile Using PCR/ESI-MS","translated_title":"","metadata":{"abstract":"ABSTRACT Background: Increasing rate and severity of C. difficile -associated disease (CDAD) have been associated with the emergence of the NAP-1 strain of C. difficile. Enhanced virulence is associated with deletions within the pathogenicity locus gene, tcdC. We hypothesize that previously known and as yet unknown mutations in the tcd gene cluster may be important for fully understanding CDAD. Methods: We have developed a PCR Electrospray Ionization Mass Spectrometry (PCR/ESI-MS) based method to rapidly identify C. difficile and detect multiple mutations in the tcdC locus, including the previously described 18 base pair deletion and single nucleotide deletion associated with NAP-1 strains, while simultaneously detecting tcdA, tcdB, cdtA and cdtB. Results: PCR/ESI-MS was performed on 65 archived C. difficile clinical isolates that had been collected during previous studies at JHH. 28 of the 65 isolates showed distinct NAP-1 like deletions in the tcdC loci. All 28 had both the 18 bp deletion and an additional single base pair deletion in the promoter region characteristic of fully virulent strains. 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In addition to detecting all previously reported mutations, it has the potential to uncover additional mutations or correlations that might help understand the virulence properties of this microbe.","publication_date":{"day":null,"month":null,"year":2008,"errors":{}}},"translated_abstract":"ABSTRACT Background: Increasing rate and severity of C. difficile -associated disease (CDAD) have been associated with the emergence of the NAP-1 strain of C. difficile. Enhanced virulence is associated with deletions within the pathogenicity locus gene, tcdC. We hypothesize that previously known and as yet unknown mutations in the tcd gene cluster may be important for fully understanding CDAD. Methods: We have developed a PCR Electrospray Ionization Mass Spectrometry (PCR/ESI-MS) based method to rapidly identify C. difficile and detect multiple mutations in the tcdC locus, including the previously described 18 base pair deletion and single nucleotide deletion associated with NAP-1 strains, while simultaneously detecting tcdA, tcdB, cdtA and cdtB. Results: PCR/ESI-MS was performed on 65 archived C. difficile clinical isolates that had been collected during previous studies at JHH. 28 of the 65 isolates showed distinct NAP-1 like deletions in the tcdC loci. All 28 had both the 18 bp deletion and an additional single base pair deletion in the promoter region characteristic of fully virulent strains. Two distinct genotypes were observed in the tcdB locus- all 28 virulent strains had a C to T SNP that has not been previously reported. Overall, of the 65 isolates tested, 54 were tcdA+/B+, with 33 showing the tcdB SNP. 4 of the 5 remaining isolates with this SNP had the previously described 36 bp deletion with unknown phenotype in the tcdC locus. Conclusion: The PCR/ESI-MS technology is a high throughput assay system useful for rapid detection and characterization of CDAD. It is capable of simultaneous identification of C. difficile and detection of several important genotypic markers that might be associated with virulence. In addition to detecting all previously reported mutations, it has the potential to uncover additional mutations or correlations that might help understand the virulence properties of this microbe.","internal_url":"https://www.academia.edu/70138686/Rapid_Detection_and_Characterization_of_the_NAP_1_Epidemic_Strain_of_Clostridium_difficile_Using_PCR_ESI_MS","translated_internal_url":"","created_at":"2022-01-31T04:22:39.239-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":25381401,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Rapid_Detection_and_Characterization_of_the_NAP_1_Epidemic_Strain_of_Clostridium_difficile_Using_PCR_ESI_MS","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":25381401,"first_name":"Ranga","middle_initials":null,"last_name":"Sampath","page_name":"RangaSampath","domain_name":"independent","created_at":"2015-01-26T21:28:55.724-08:00","display_name":"Ranga Sampath","url":"https://independent.academia.edu/RangaSampath"},"attachments":[],"research_interests":[{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"}],"urls":[]}, dispatcherData: dispatcherData }); 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="58685989"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/58685989/Diagnostic_Infectious_Diseases_Testing_Outside_Clinics_A_Global_Systematic_Review_and_Meta_analysis"><img alt="Research paper thumbnail of Diagnostic Infectious Diseases Testing Outside Clinics: A Global Systematic Review and Meta-analysis" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/58685989/Diagnostic_Infectious_Diseases_Testing_Outside_Clinics_A_Global_Systematic_Review_and_Meta_analysis">Diagnostic Infectious Diseases Testing Outside Clinics: A Global Systematic Review and Meta-analysis</a></div><div class="wp-workCard_item"><span>Open Forum Infectious Diseases</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Background Most people around the world do not have access to facility-based diagnostic testing, ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Background Most people around the world do not have access to facility-based diagnostic testing, and the gap in availability of diagnostic tests is a major public health challenge. Self-testing, self-sampling, and institutional testing outside conventional clinical settings are transforming infectious disease diagnostic testing in a wide range of low- and middle-income countries (LMICs). We examined the delivery models of infectious disease diagnostic testing outside clinics to assess the impact on test uptake and linkage to care. Methods We conducted a systematic review and meta-analysis, searching 6 databases and including original research manuscripts comparing testing outside clinics with conventional testing. The main outcomes were test uptake and linkage to care, delivery models, and adverse outcomes. Data from studies with similar interventions and outcomes within thematic areas of interest were pooled, and the quality of evidence was assessed using GRADE. This study was regi...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="58685989"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="58685989"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 58685989; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=58685989]").text(description); $(".js-view-count[data-work-id=58685989]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 58685989; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='58685989']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 58685989, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=58685989]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":58685989,"title":"Diagnostic Infectious Diseases Testing Outside Clinics: A Global Systematic Review and Meta-analysis","translated_title":"","metadata":{"abstract":"Background Most people around the world do not have access to facility-based diagnostic testing, and the gap in availability of diagnostic tests is a major public health challenge. Self-testing, self-sampling, and institutional testing outside conventional clinical settings are transforming infectious disease diagnostic testing in a wide range of low- and middle-income countries (LMICs). We examined the delivery models of infectious disease diagnostic testing outside clinics to assess the impact on test uptake and linkage to care. Methods We conducted a systematic review and meta-analysis, searching 6 databases and including original research manuscripts comparing testing outside clinics with conventional testing. The main outcomes were test uptake and linkage to care, delivery models, and adverse outcomes. Data from studies with similar interventions and outcomes within thematic areas of interest were pooled, and the quality of evidence was assessed using GRADE. This study was regi...","publisher":"Oxford University Press (OUP)","publication_name":"Open Forum Infectious Diseases"},"translated_abstract":"Background Most people around the world do not have access to facility-based diagnostic testing, and the gap in availability of diagnostic tests is a major public health challenge. Self-testing, self-sampling, and institutional testing outside conventional clinical settings are transforming infectious disease diagnostic testing in a wide range of low- and middle-income countries (LMICs). We examined the delivery models of infectious disease diagnostic testing outside clinics to assess the impact on test uptake and linkage to care. Methods We conducted a systematic review and meta-analysis, searching 6 databases and including original research manuscripts comparing testing outside clinics with conventional testing. The main outcomes were test uptake and linkage to care, delivery models, and adverse outcomes. Data from studies with similar interventions and outcomes within thematic areas of interest were pooled, and the quality of evidence was assessed using GRADE. This study was regi...","internal_url":"https://www.academia.edu/58685989/Diagnostic_Infectious_Diseases_Testing_Outside_Clinics_A_Global_Systematic_Review_and_Meta_analysis","translated_internal_url":"","created_at":"2021-10-17T19:34:31.967-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":25381401,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Diagnostic_Infectious_Diseases_Testing_Outside_Clinics_A_Global_Systematic_Review_and_Meta_analysis","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":25381401,"first_name":"Ranga","middle_initials":null,"last_name":"Sampath","page_name":"RangaSampath","domain_name":"independent","created_at":"2015-01-26T21:28:55.724-08:00","display_name":"Ranga Sampath","url":"https://independent.academia.edu/RangaSampath"},"attachments":[],"research_interests":[],"urls":[{"id":13280128,"url":"http://academic.oup.com/ofid/advance-article-pdf/doi/10.1093/ofid/ofaa360/33663102/ofaa360.pdf"}]}, dispatcherData: dispatcherData }); 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An Analysis of Data from PRIMERS-II of the Antibiotic Resistance Leadership Group (ARLG)</a></div><div class="wp-workCard_item"><span>Open Forum Infectious Diseases</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="cc8b81392c4d97ff3e4c5ef5e4d1d738" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":72976709,"asset_id":58685987,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/72976709/download_file?st=MTczMjQyMjk1Myw4LjIyMi4yMDguMTQ2&st=MTczMjQyMjk1Myw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="58685987"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="58685987"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 58685987; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=58685987]").text(description); $(".js-view-count[data-work-id=58685987]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 58685987; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='58685987']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 58685987, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "cc8b81392c4d97ff3e4c5ef5e4d1d738" } } $('.js-work-strip[data-work-id=58685987]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":58685987,"title":"608Can Rapid Molecular Diagnostics Assist in the Choice of b-Lactam Antibiotics? 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="58685986"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/58685986/Acute_Respiratory_Viral_Infection_Among_Outpatient_Healthcare_Personnel"><img alt="Research paper thumbnail of Acute Respiratory Viral Infection Among Outpatient Healthcare Personnel" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/58685986/Acute_Respiratory_Viral_Infection_Among_Outpatient_Healthcare_Personnel">Acute Respiratory Viral Infection Among Outpatient Healthcare Personnel</a></div><div class="wp-workCard_item"><span>Open Forum Infectious Diseases</span><span>, 2015</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">ABSTRACT Background: Respiratory disease is a common source of morbidity among healthcare personn...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">ABSTRACT Background: Respiratory disease is a common source of morbidity among healthcare personnel (HCP). However, the causes of acute respiratory illness (ARI) have not been well studied. We assessed the viral causes of respiratory illness among HCP enrolled in a cluster randomized clinical trial at 99 outpatient departments and emergency departments in 7 locations: Baltimore, MD; Denver, CO (3); Houston, TX; New York, NY; and Washington, DC. Methods: During 12 weeks of the 2011-2 (YR1) and 2012-3 (YR2) respiratory seasons, HCP were surveyed for signs and symptoms of ARI. Participants with symptoms were cultured and swabs frozen at -800C. Also, two random swabs were obtained during the intervention period. Samples were tested using a multiplex PCR for 13 viruses (PCR/ESI-MS, Abbott labs). Paired blood samples were obtained prior to the start of the intervention period and 2-weeks post-intervention, then tested for influenza antibodies (&amp;gt;2-fold antibody increase). YR2 serum samples have not been tested. Results: Among 1686 participants (609 YR1; 1077 YR2), 4255 swabs were obtained. To date, 1579 swab samples have been tested (1370 YR1; 209 YR2): 544 symptomatic (335 YR1; 209 YR2) and 1035 asymptomatic. Influenza antibodies were tested in 1292 serum samples. Based on testing, 102 YR1 and 76 YR2 samples revealed the following viral isolates: influenza A (36), influenza B (8), parainfluenza (2), adenovirus (4), coronavirus (78), metapneumovirus (16), rhinovirus (17), and respiratory syncytial virus (RSV, 17) (Figure 1). Four cases of influenza A were identified by serological and viral testing (Figure 2). Conclusion: ARIs are common among HCP with 25% developing symptoms during the respiratory viral season. Among ARI in HCP, 27% had identifiable viral causes – most commonly coronavirus at 44%. This increased in YR2 with identification of rhinovirus. Most interesting is the significant number of asymptomatic HCP (19%) with identifiable virus. Some of these viruses cause significant morbidity to patients and transmission from HCP is critical to patient safety and that of the HCP themselves. *** Figure 1 Figure 2: Results show both serologic and nose/throat sample results. *Rhinovirus not tested. **Data available for 2 sites, asymptomatic data pending. ***NOTE: The data analysis is ongoing - more information will be available at the presentation.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="58685986"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="58685986"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 58685986; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=58685986]").text(description); $(".js-view-count[data-work-id=58685986]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 58685986; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='58685986']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 58685986, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=58685986]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":58685986,"title":"Acute Respiratory Viral Infection Among Outpatient Healthcare Personnel","translated_title":"","metadata":{"abstract":"ABSTRACT Background: Respiratory disease is a common source of morbidity among healthcare personnel (HCP). However, the causes of acute respiratory illness (ARI) have not been well studied. We assessed the viral causes of respiratory illness among HCP enrolled in a cluster randomized clinical trial at 99 outpatient departments and emergency departments in 7 locations: Baltimore, MD; Denver, CO (3); Houston, TX; New York, NY; and Washington, DC. Methods: During 12 weeks of the 2011-2 (YR1) and 2012-3 (YR2) respiratory seasons, HCP were surveyed for signs and symptoms of ARI. Participants with symptoms were cultured and swabs frozen at -800C. Also, two random swabs were obtained during the intervention period. Samples were tested using a multiplex PCR for 13 viruses (PCR/ESI-MS, Abbott labs). Paired blood samples were obtained prior to the start of the intervention period and 2-weeks post-intervention, then tested for influenza antibodies (\u0026amp;gt;2-fold antibody increase). YR2 serum samples have not been tested. Results: Among 1686 participants (609 YR1; 1077 YR2), 4255 swabs were obtained. To date, 1579 swab samples have been tested (1370 YR1; 209 YR2): 544 symptomatic (335 YR1; 209 YR2) and 1035 asymptomatic. Influenza antibodies were tested in 1292 serum samples. Based on testing, 102 YR1 and 76 YR2 samples revealed the following viral isolates: influenza A (36), influenza B (8), parainfluenza (2), adenovirus (4), coronavirus (78), metapneumovirus (16), rhinovirus (17), and respiratory syncytial virus (RSV, 17) (Figure 1). Four cases of influenza A were identified by serological and viral testing (Figure 2). Conclusion: ARIs are common among HCP with 25% developing symptoms during the respiratory viral season. Among ARI in HCP, 27% had identifiable viral causes – most commonly coronavirus at 44%. This increased in YR2 with identification of rhinovirus. Most interesting is the significant number of asymptomatic HCP (19%) with identifiable virus. Some of these viruses cause significant morbidity to patients and transmission from HCP is critical to patient safety and that of the HCP themselves. *** Figure 1 Figure 2: Results show both serologic and nose/throat sample results. *Rhinovirus not tested. **Data available for 2 sites, asymptomatic data pending. ***NOTE: The data analysis is ongoing - more information will be available at the presentation.","publisher":"Oxford University Press (OUP)","publication_date":{"day":null,"month":null,"year":2015,"errors":{}},"publication_name":"Open Forum Infectious Diseases"},"translated_abstract":"ABSTRACT Background: Respiratory disease is a common source of morbidity among healthcare personnel (HCP). However, the causes of acute respiratory illness (ARI) have not been well studied. We assessed the viral causes of respiratory illness among HCP enrolled in a cluster randomized clinical trial at 99 outpatient departments and emergency departments in 7 locations: Baltimore, MD; Denver, CO (3); Houston, TX; New York, NY; and Washington, DC. Methods: During 12 weeks of the 2011-2 (YR1) and 2012-3 (YR2) respiratory seasons, HCP were surveyed for signs and symptoms of ARI. Participants with symptoms were cultured and swabs frozen at -800C. Also, two random swabs were obtained during the intervention period. Samples were tested using a multiplex PCR for 13 viruses (PCR/ESI-MS, Abbott labs). Paired blood samples were obtained prior to the start of the intervention period and 2-weeks post-intervention, then tested for influenza antibodies (\u0026amp;gt;2-fold antibody increase). YR2 serum samples have not been tested. Results: Among 1686 participants (609 YR1; 1077 YR2), 4255 swabs were obtained. To date, 1579 swab samples have been tested (1370 YR1; 209 YR2): 544 symptomatic (335 YR1; 209 YR2) and 1035 asymptomatic. Influenza antibodies were tested in 1292 serum samples. Based on testing, 102 YR1 and 76 YR2 samples revealed the following viral isolates: influenza A (36), influenza B (8), parainfluenza (2), adenovirus (4), coronavirus (78), metapneumovirus (16), rhinovirus (17), and respiratory syncytial virus (RSV, 17) (Figure 1). Four cases of influenza A were identified by serological and viral testing (Figure 2). Conclusion: ARIs are common among HCP with 25% developing symptoms during the respiratory viral season. Among ARI in HCP, 27% had identifiable viral causes – most commonly coronavirus at 44%. This increased in YR2 with identification of rhinovirus. Most interesting is the significant number of asymptomatic HCP (19%) with identifiable virus. Some of these viruses cause significant morbidity to patients and transmission from HCP is critical to patient safety and that of the HCP themselves. *** Figure 1 Figure 2: Results show both serologic and nose/throat sample results. *Rhinovirus not tested. **Data available for 2 sites, asymptomatic data pending. ***NOTE: The data analysis is ongoing - more information will be available at the presentation.","internal_url":"https://www.academia.edu/58685986/Acute_Respiratory_Viral_Infection_Among_Outpatient_Healthcare_Personnel","translated_internal_url":"","created_at":"2021-10-17T19:34:31.629-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":25381401,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Acute_Respiratory_Viral_Infection_Among_Outpatient_Healthcare_Personnel","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":25381401,"first_name":"Ranga","middle_initials":null,"last_name":"Sampath","page_name":"RangaSampath","domain_name":"independent","created_at":"2015-01-26T21:28:55.724-08:00","display_name":"Ranga Sampath","url":"https://independent.academia.edu/RangaSampath"},"attachments":[],"research_interests":[],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="58685985"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/58685985/Rapid_Molecular_Diagnostics_Antibiotic_Treatment_Decisions_and_Developing_Approaches_to_Inform_Empiric_Therapy_PRIMERS_I_and_II"><img alt="Research paper thumbnail of Rapid Molecular Diagnostics, Antibiotic Treatment Decisions, and Developing Approaches to Inform Empiric Therapy: PRIMERS I and II" class="work-thumbnail" src="https://attachments.academia-assets.com/72976694/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/58685985/Rapid_Molecular_Diagnostics_Antibiotic_Treatment_Decisions_and_Developing_Approaches_to_Inform_Empiric_Therapy_PRIMERS_I_and_II">Rapid Molecular Diagnostics, Antibiotic Treatment Decisions, and Developing Approaches to Inform Empiric Therapy: PRIMERS I and II</a></div><div class="wp-workCard_item"><span>Clinical infectious diseases : an official publication of the Infectious Diseases Society of America</span><span>, Jan 25, 2015</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated"> Rapid molecular diagnostic (RMD) platforms may lead to better antimicrobial stewardship. Our obj...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden"> Rapid molecular diagnostic (RMD) platforms may lead to better antimicrobial stewardship. Our objective was to develop an analytic strategy to enhance the interpretation of RMDs for the clinician. We compared the performance characteristics of four RMD platforms for detecting resistance against β-lactams in 72 highly drug-resistant isolates of Escherichia coli and Klebsiella pneumoniae (PRIMERS I). Subsequently, two platforms were utilized in a blinded study examining a collection of 196 isolates of E. coli and K. pneumoniae (PRIMERS II). We evaluated the genotypic results as predictors of resistance or susceptibility against a panel of common β-lactam antibiotics. Analytical strategies were designed to include graphical representations of platform performance: i) discrimination summary plots; and ii) determination of susceptibility and resistance predictive values that can be readily interpreted by skilled practitioners to help inform decision making. In PRIMERS I, the four RMD p...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="9afe79d2c83c6b891ed2c12e7de69155" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":72976694,"asset_id":58685985,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/72976694/download_file?st=MTczMjQyMjk1Myw4LjIyMi4yMDguMTQ2&st=MTczMjQyMjk1Myw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="58685985"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="58685985"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 58685985; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=58685985]").text(description); $(".js-view-count[data-work-id=58685985]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 58685985; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='58685985']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 58685985, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "9afe79d2c83c6b891ed2c12e7de69155" } } $('.js-work-strip[data-work-id=58685985]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":58685985,"title":"Rapid Molecular Diagnostics, Antibiotic Treatment Decisions, and Developing Approaches to Inform Empiric Therapy: PRIMERS I and II","translated_title":"","metadata":{"abstract":" Rapid molecular diagnostic (RMD) platforms may lead to better antimicrobial stewardship. Our objective was to develop an analytic strategy to enhance the interpretation of RMDs for the clinician. We compared the performance characteristics of four RMD platforms for detecting resistance against β-lactams in 72 highly drug-resistant isolates of Escherichia coli and Klebsiella pneumoniae (PRIMERS I). Subsequently, two platforms were utilized in a blinded study examining a collection of 196 isolates of E. coli and K. pneumoniae (PRIMERS II). We evaluated the genotypic results as predictors of resistance or susceptibility against a panel of common β-lactam antibiotics. Analytical strategies were designed to include graphical representations of platform performance: i) discrimination summary plots; and ii) determination of susceptibility and resistance predictive values that can be readily interpreted by skilled practitioners to help inform decision making. In PRIMERS I, the four RMD p...","publication_date":{"day":25,"month":1,"year":2015,"errors":{}},"publication_name":"Clinical infectious diseases : an official publication of the Infectious Diseases Society of America"},"translated_abstract":" Rapid molecular diagnostic (RMD) platforms may lead to better antimicrobial stewardship. Our objective was to develop an analytic strategy to enhance the interpretation of RMDs for the clinician. We compared the performance characteristics of four RMD platforms for detecting resistance against β-lactams in 72 highly drug-resistant isolates of Escherichia coli and Klebsiella pneumoniae (PRIMERS I). Subsequently, two platforms were utilized in a blinded study examining a collection of 196 isolates of E. coli and K. pneumoniae (PRIMERS II). We evaluated the genotypic results as predictors of resistance or susceptibility against a panel of common β-lactam antibiotics. Analytical strategies were designed to include graphical representations of platform performance: i) discrimination summary plots; and ii) determination of susceptibility and resistance predictive values that can be readily interpreted by skilled practitioners to help inform decision making. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="58685984"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/58685984/A_Broad_Range_Polymerase_Chain_Reaction_Coupled_to_Electrospray_Ionization_Mass_Spectrometry_for_Detection_of_Biothreat_Agents_and_Common_Pathogens_in_Respiratory_Samples"><img alt="Research paper thumbnail of A Broad-Range Polymerase Chain Reaction Coupled to Electrospray Ionization Mass Spectrometry for Detection of Biothreat Agents and Common Pathogens in Respiratory Samples" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/58685984/A_Broad_Range_Polymerase_Chain_Reaction_Coupled_to_Electrospray_Ionization_Mass_Spectrometry_for_Detection_of_Biothreat_Agents_and_Common_Pathogens_in_Respiratory_Samples">A Broad-Range Polymerase Chain Reaction Coupled to Electrospray Ionization Mass Spectrometry for Detection of Biothreat Agents and Common Pathogens in Respiratory Samples</a></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Background: Biological threat (BT) agent infections (i.e. Anthrax, Plague, and Tularemia) often p...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Background: Biological threat (BT) agent infections (i.e. Anthrax, Plague, and Tularemia) often present with symptoms similar to other common respiratory infections (i.e. influenza like illness). New diagnostic platforms for respiratory pathogens must be capable of sensitive, specific, and rapid differentiation of BT organisms and common pathogens from normal flora. Base composition characterization by electrospray ionization mass spectrometry (ESI-MS) of nucleic acids amplified via RT-PCR from human respiratory samples allows for broad pathogen identification within eight hours after collection. In this study, we investigated the performance characteristics of RT-PCR/ESI-MS for distinguishing BT agents and common bacterial, fungal, and viral respiratory pathogens from normal flora in clinical samples from subjects with suspected respiratory infections. Methods: Bronchoalveolar lavages (BALs) and nasopharyngeal (NP) swab samples were collected from patients with acute respiratory in...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="58685984"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="58685984"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 58685984; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=58685984]").text(description); $(".js-view-count[data-work-id=58685984]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 58685984; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='58685984']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 58685984, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=58685984]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":58685984,"title":"A Broad-Range Polymerase Chain Reaction Coupled to Electrospray Ionization Mass Spectrometry for Detection of Biothreat Agents and Common Pathogens in Respiratory Samples","translated_title":"","metadata":{"abstract":"Background: Biological threat (BT) agent infections (i.e. Anthrax, Plague, and Tularemia) often present with symptoms similar to other common respiratory infections (i.e. influenza like illness). New diagnostic platforms for respiratory pathogens must be capable of sensitive, specific, and rapid differentiation of BT organisms and common pathogens from normal flora. Base composition characterization by electrospray ionization mass spectrometry (ESI-MS) of nucleic acids amplified via RT-PCR from human respiratory samples allows for broad pathogen identification within eight hours after collection. In this study, we investigated the performance characteristics of RT-PCR/ESI-MS for distinguishing BT agents and common bacterial, fungal, and viral respiratory pathogens from normal flora in clinical samples from subjects with suspected respiratory infections. Methods: Bronchoalveolar lavages (BALs) and nasopharyngeal (NP) swab samples were collected from patients with acute respiratory in..."},"translated_abstract":"Background: Biological threat (BT) agent infections (i.e. Anthrax, Plague, and Tularemia) often present with symptoms similar to other common respiratory infections (i.e. influenza like illness). New diagnostic platforms for respiratory pathogens must be capable of sensitive, specific, and rapid differentiation of BT organisms and common pathogens from normal flora. Base composition characterization by electrospray ionization mass spectrometry (ESI-MS) of nucleic acids amplified via RT-PCR from human respiratory samples allows for broad pathogen identification within eight hours after collection. In this study, we investigated the performance characteristics of RT-PCR/ESI-MS for distinguishing BT agents and common bacterial, fungal, and viral respiratory pathogens from normal flora in clinical samples from subjects with suspected respiratory infections. Methods: Bronchoalveolar lavages (BALs) and nasopharyngeal (NP) swab samples were collected from patients with acute respiratory in...","internal_url":"https://www.academia.edu/58685984/A_Broad_Range_Polymerase_Chain_Reaction_Coupled_to_Electrospray_Ionization_Mass_Spectrometry_for_Detection_of_Biothreat_Agents_and_Common_Pathogens_in_Respiratory_Samples","translated_internal_url":"","created_at":"2021-10-17T19:34:31.410-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":25381401,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"A_Broad_Range_Polymerase_Chain_Reaction_Coupled_to_Electrospray_Ionization_Mass_Spectrometry_for_Detection_of_Biothreat_Agents_and_Common_Pathogens_in_Respiratory_Samples","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":25381401,"first_name":"Ranga","middle_initials":null,"last_name":"Sampath","page_name":"RangaSampath","domain_name":"independent","created_at":"2015-01-26T21:28:55.724-08:00","display_name":"Ranga Sampath","url":"https://independent.academia.edu/RangaSampath"},"attachments":[],"research_interests":[],"urls":[]}, dispatcherData: dispatcherData }); 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Studies have shown the genotype of Borrelia burgdorferi sensu stricto (s.s.) or the species of B. burgdorferi sensu lato (s.l.) affects the ability of the bacteria to cause local or disseminated infection in humans. Methodology/Principal Findings: We used a multilocus PCR electrospray mass spectrometry assay to determine the species and genotype Borrelia from ticks collected in New York, Connecticut, Indiana, Southern Germany, and California and characterized isolates from parts of the United States and Europe. These analyses identified 53 distinct genotypes of B. burgdorferi sensu stricto with higher resolution than ospC typing. Genotypes of other members of the B. burgdorferi sensu lato complex were also identified and genotyped including B. afzelii, B. garinii, B. lusitaniae, B. spielmanii, and B. valaisiana. While each site in North America had genotypes unique to that location, we found genotypes shared between individual regions and two genotypes found across the United States. Significant B. burgdorferi s.s. genotypic diversity was observed between North America and Europe: only 6.6% of US genotypes (3 of 45) were found in Europe and 27% of the European genotypes (3 of 11) were observed in the US. Interestingly, 39% of adult Ixodes scapularis ticks from North America were infected with more than one genotype of B. burgdorferi s.s. and 22.2% of Ixodes ricinus ticks from Germany were infected with more than one genotype of B. burgdorferi s.l. Conclusions/Significance: The presence of multiple Borrelia genotypes in ticks increases the probability that a person will be infected with more than one genotype of B. burgdorferi, potentially increasing the risks of disseminated Lyme disease. Our study indicates that the genotypic diversity of Borrelia in ticks in both North America and Europe is higher then previously reported and can have potential clinical consequences.","publication_date":{"day":null,"month":null,"year":2010,"errors":{}},"publication_name":"PLoS ONE","grobid_abstract_attachment_id":72976738},"translated_abstract":null,"internal_url":"https://www.academia.edu/58685982/Genotypic_Variation_and_Mixtures_of_Lyme_Borrelia_in_Ixodes_Ticks_from_North_America_and_Europe","translated_internal_url":"","created_at":"2021-10-17T19:34:31.303-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":25381401,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":72976738,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/72976738/thumbnails/1.jpg","file_name":"Genotypic_variation_and_mixtures_of_Lyme20211017-13084-1y304xx.pdf","download_url":"https://www.academia.edu/attachments/72976738/download_file?st=MTczMjQyMjk1Myw4LjIyMi4yMDguMTQ2&st=MTczMjQyMjk1Myw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Genotypic_Variation_and_Mixtures_of_Lyme.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/72976738/Genotypic_variation_and_mixtures_of_Lyme20211017-13084-1y304xx.pdf?1634524576=\u0026response-content-disposition=attachment%3B+filename%3DGenotypic_Variation_and_Mixtures_of_Lyme.pdf\u0026Expires=1732426553\u0026Signature=Tf6fzoIb6J~0d64pXlrnRbYN3T1rujhPOZXySutTwlKItw1fkWsFlxcU1q0Bqq-YZ3EHg4G6sRzYAFvbIe1LNIbcRn4G75fgIcOrzhXNbyr04K-JLM2DZxnl-U0io83B~sWGNZ1Bw6PUz3VIeIGXcBUQqu-TyPBnPVR8bIU1NpySttYcPchbImOfPvRRzqdYpjLc-35zzr9fgWL6rm38mbXMSZHxiw40tNRSmx-mq7klnom~jClzw-yJ-FqBWtKBirM0Kcd~wWtQm6~EWU2S1CLkpo8zk8IxwYv2O0yljuHO4PaRaRue6DCX27si2YPBXbl4ZB7Yg82jlIK89mNW~g__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Genotypic_Variation_and_Mixtures_of_Lyme_Borrelia_in_Ixodes_Ticks_from_North_America_and_Europe","translated_slug":"","page_count":9,"language":"en","content_type":"Work","owner":{"id":25381401,"first_name":"Ranga","middle_initials":null,"last_name":"Sampath","page_name":"RangaSampath","domain_name":"independent","created_at":"2015-01-26T21:28:55.724-08:00","display_name":"Ranga Sampath","url":"https://independent.academia.edu/RangaSampath"},"attachments":[{"id":72976738,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/72976738/thumbnails/1.jpg","file_name":"Genotypic_variation_and_mixtures_of_Lyme20211017-13084-1y304xx.pdf","download_url":"https://www.academia.edu/attachments/72976738/download_file?st=MTczMjQyMjk1Myw4LjIyMi4yMDguMTQ2&st=MTczMjQyMjk1Myw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Genotypic_Variation_and_Mixtures_of_Lyme.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/72976738/Genotypic_variation_and_mixtures_of_Lyme20211017-13084-1y304xx.pdf?1634524576=\u0026response-content-disposition=attachment%3B+filename%3DGenotypic_Variation_and_Mixtures_of_Lyme.pdf\u0026Expires=1732426553\u0026Signature=Tf6fzoIb6J~0d64pXlrnRbYN3T1rujhPOZXySutTwlKItw1fkWsFlxcU1q0Bqq-YZ3EHg4G6sRzYAFvbIe1LNIbcRn4G75fgIcOrzhXNbyr04K-JLM2DZxnl-U0io83B~sWGNZ1Bw6PUz3VIeIGXcBUQqu-TyPBnPVR8bIU1NpySttYcPchbImOfPvRRzqdYpjLc-35zzr9fgWL6rm38mbXMSZHxiw40tNRSmx-mq7klnom~jClzw-yJ-FqBWtKBirM0Kcd~wWtQm6~EWU2S1CLkpo8zk8IxwYv2O0yljuHO4PaRaRue6DCX27si2YPBXbl4ZB7Yg82jlIK89mNW~g__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":48,"name":"Engineering","url":"https://www.academia.edu/Documents/in/Engineering"},{"id":261,"name":"Geography","url":"https://www.academia.edu/Documents/in/Geography"},{"id":523,"name":"Chemistry","url":"https://www.academia.edu/Documents/in/Chemistry"},{"id":5769,"name":"Mass Spectrometry","url":"https://www.academia.edu/Documents/in/Mass_Spectrometry"},{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":28235,"name":"Multidisciplinary","url":"https://www.academia.edu/Documents/in/Multidisciplinary"},{"id":53198,"name":"Lyme disease","url":"https://www.academia.edu/Documents/in/Lyme_disease"},{"id":64568,"name":"Humans","url":"https://www.academia.edu/Documents/in/Humans"},{"id":75826,"name":"Europe","url":"https://www.academia.edu/Documents/in/Europe"},{"id":99234,"name":"Animals","url":"https://www.academia.edu/Documents/in/Animals"},{"id":128172,"name":"Borrelia burgdorferi","url":"https://www.academia.edu/Documents/in/Borrelia_burgdorferi"},{"id":361848,"name":"Ixodes ricinus","url":"https://www.academia.edu/Documents/in/Ixodes_ricinus"},{"id":372410,"name":"Genotype","url":"https://www.academia.edu/Documents/in/Genotype"},{"id":577933,"name":"Genetic variation","url":"https://www.academia.edu/Documents/in/Genetic_variation"},{"id":756265,"name":"Borrelia","url":"https://www.academia.edu/Documents/in/Borrelia"},{"id":897281,"name":"Ixodes scapularis","url":"https://www.academia.edu/Documents/in/Ixodes_scapularis"},{"id":2457889,"name":"Genotypic diversity","url":"https://www.academia.edu/Documents/in/Genotypic_diversity"},{"id":2693601,"name":"Genotypic variation","url":"https://www.academia.edu/Documents/in/Genotypic_variation"},{"id":3114383,"name":"Ixodes","url":"https://www.academia.edu/Documents/in/Ixodes"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="58685981"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/58685981/Rev_response_elements_RRE_in_lentiviruses_An_RNAMotif_algorithm_based_strategy_for_RRE_prediction"><img alt="Research paper thumbnail of Rev response elements (RRE) in lentiviruses: An RNAMotif algorithm-based strategy for RRE prediction" class="work-thumbnail" src="https://attachments.academia-assets.com/72976753/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/58685981/Rev_response_elements_RRE_in_lentiviruses_An_RNAMotif_algorithm_based_strategy_for_RRE_prediction">Rev response elements (RRE) in lentiviruses: An RNAMotif algorithm-based strategy for RRE prediction</a></div><div class="wp-workCard_item"><span>Medicinal Research Reviews</span><span>, 2002</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="0e075c2961f18347f3a95eb80898a613" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":72976753,"asset_id":58685981,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/72976753/download_file?st=MTczMjQyMjk1Myw4LjIyMi4yMDguMTQ2&st=MTczMjQyMjk1Myw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="58685981"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa 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$('.js-work-strip[data-work-id=58685981]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":58685981,"title":"Rev response elements (RRE) in lentiviruses: An RNAMotif algorithm-based strategy for RRE prediction","translated_title":"","metadata":{"publisher":"Wiley","grobid_abstract":"Lentiviruses (a sub-family of the retroviridae family) include primate and non-primate viruses associated with chronic diseases of the immune system and the central nervous system. All lentiviruses encode a regulatory protein Rev that is essential for post-transcriptional transport of the unspliced and incompletely spliced viral mRNAs from nuclei to cytoplasm. The Rev protein acts via binding to an RNA structural element known as the Rev responsive element (RRE). The RRE location and structure and the mechanism of the Rev-RRE interaction in primate and non-primate lentiviruses have been analyzed and compared. Based on structural data available for RRE of HIV-1, a two step computational strategy for prediction of putative RRE regions in lentivirus genomes has been developed. First, the RNAMotif algorithm was used to search genomic sequence for highly structured regions (HSR). Then the program RNAstructure, version 3.6 was used to calculate the structure and thermodynamic stability of the region of $ 350 nucleotides encompassing the HSR. Our strategy correctly predicted the locations of all previously reported lentivirus RREs. We were able also to predict the locations and structures of potential RREs in four additional lentiviruses.","publication_date":{"day":null,"month":null,"year":2002,"errors":{}},"publication_name":"Medicinal Research Reviews","grobid_abstract_attachment_id":72976753},"translated_abstract":null,"internal_url":"https://www.academia.edu/58685981/Rev_response_elements_RRE_in_lentiviruses_An_RNAMotif_algorithm_based_strategy_for_RRE_prediction","translated_internal_url":"","created_at":"2021-10-17T19:34:31.197-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":25381401,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":72976753,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/72976753/thumbnails/1.jpg","file_name":"med.1002720211017-5334-19owgn5.pdf","download_url":"https://www.academia.edu/attachments/72976753/download_file?st=MTczMjQyMjk1Myw4LjIyMi4yMDguMTQ2&st=MTczMjQyMjk1Myw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Rev_response_elements_RRE_in_lentiviruse.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/72976753/med.1002720211017-5334-19owgn5-libre.pdf?1634525432=\u0026response-content-disposition=attachment%3B+filename%3DRev_response_elements_RRE_in_lentiviruse.pdf\u0026Expires=1732426553\u0026Signature=dStq1fOnDQK6kRcPMH~JwlKnmtN0b~6nEtrRkayR3lUQM1Ga5cq7RVKQIOZebisvCVE~b2OKHNmJ-c1jJqE-jcpA07s1ucy7Y6aXIRHsTXZnJxP3shO15T9dWySwYwvv-wPkJHQ7bM6Ts~XTTt6J3l1NwIlMWOBzufIlaVd5vGhfY74tuTzKMhQE7EU9RCT7Os0OR0ClN2wg7osYS3YS-VY2N6m46Xhy5k~aK6wpgDPkRj-~CHSYLZ4t8izH~Dq1xUfufTz-6HA49Tam1KVvAGKHYdkY~EzX9kJJFNud-PBTA9yF-YzmLgulPV-IyvnoV9~67gWvlpDEz2b5y5zPCA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Rev_response_elements_RRE_in_lentiviruses_An_RNAMotif_algorithm_based_strategy_for_RRE_prediction","translated_slug":"","page_count":20,"language":"en","content_type":"Work","owner":{"id":25381401,"first_name":"Ranga","middle_initials":null,"last_name":"Sampath","page_name":"RangaSampath","domain_name":"independent","created_at":"2015-01-26T21:28:55.724-08:00","display_name":"Ranga Sampath","url":"https://independent.academia.edu/RangaSampath"},"attachments":[{"id":72976753,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/72976753/thumbnails/1.jpg","file_name":"med.1002720211017-5334-19owgn5.pdf","download_url":"https://www.academia.edu/attachments/72976753/download_file?st=MTczMjQyMjk1Myw4LjIyMi4yMDguMTQ2&st=MTczMjQyMjk1Myw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Rev_response_elements_RRE_in_lentiviruse.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/72976753/med.1002720211017-5334-19owgn5-libre.pdf?1634525432=\u0026response-content-disposition=attachment%3B+filename%3DRev_response_elements_RRE_in_lentiviruse.pdf\u0026Expires=1732426553\u0026Signature=dStq1fOnDQK6kRcPMH~JwlKnmtN0b~6nEtrRkayR3lUQM1Ga5cq7RVKQIOZebisvCVE~b2OKHNmJ-c1jJqE-jcpA07s1ucy7Y6aXIRHsTXZnJxP3shO15T9dWySwYwvv-wPkJHQ7bM6Ts~XTTt6J3l1NwIlMWOBzufIlaVd5vGhfY74tuTzKMhQE7EU9RCT7Os0OR0ClN2wg7osYS3YS-VY2N6m46Xhy5k~aK6wpgDPkRj-~CHSYLZ4t8izH~Dq1xUfufTz-6HA49Tam1KVvAGKHYdkY~EzX9kJJFNud-PBTA9yF-YzmLgulPV-IyvnoV9~67gWvlpDEz2b5y5zPCA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":428,"name":"Algorithms","url":"https://www.academia.edu/Documents/in/Algorithms"},{"id":522,"name":"Thermodynamics","url":"https://www.academia.edu/Documents/in/Thermodynamics"},{"id":4233,"name":"Computational Biology","url":"https://www.academia.edu/Documents/in/Computational_Biology"},{"id":39978,"name":"HIV","url":"https://www.academia.edu/Documents/in/HIV"},{"id":52714,"name":"Primates","url":"https://www.academia.edu/Documents/in/Primates"},{"id":64568,"name":"Humans","url":"https://www.academia.edu/Documents/in/Humans"},{"id":99234,"name":"Animals","url":"https://www.academia.edu/Documents/in/Animals"},{"id":140613,"name":"Lentivirus","url":"https://www.academia.edu/Documents/in/Lentivirus"},{"id":1643624,"name":"Response Elements","url":"https://www.academia.edu/Documents/in/Response_Elements"},{"id":3789884,"name":"Pharmacology and pharmaceutical sciences","url":"https://www.academia.edu/Documents/in/Pharmacology_and_pharmaceutical_sciences"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="58685980"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/58685980/Detection_of_heartworm_infection_in_dogs_via_PCR_amplification_and_electrospray_ionization_mass_spectrometry_of_nucleic_acid_extracts_from_whole_blood_samples"><img alt="Research paper thumbnail of Detection of heartworm infection in dogs via PCR amplification and electrospray ionization mass spectrometry of nucleic acid extracts from whole blood samples" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/58685980/Detection_of_heartworm_infection_in_dogs_via_PCR_amplification_and_electrospray_ionization_mass_spectrometry_of_nucleic_acid_extracts_from_whole_blood_samples">Detection of heartworm infection in dogs via PCR amplification and electrospray ionization mass spectrometry of nucleic acid extracts from whole blood samples</a></div><div class="wp-workCard_item"><span>American Journal of Veterinary Research</span><span>, 2012</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">To develop and evaluate a rapid and accurate assay involving PCR amplification and electrospray i...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">To develop and evaluate a rapid and accurate assay involving PCR amplification and electrospray ionization mass spectrometry of nucleic acid extracts from whole blood samples for the detection of Dirofilaria immitis infection in dogs. Whole blood nucleic acid extracts from 29 dogs experimentally infected with D immitis (and in which circulating D immitis antigen was detected) and 10 uninfected dogs. 16 of the 29 whole blood samples from infected dogs were examined at the time of collection for circulating microfilaria. Nucleic acids were extracted from all whole blood specimens and underwent PCR amplification with 12 PCR primer pairs designed to detect a wide range of pathogens (including the Wolbachia endosymbiont of D immitis) and electrospray ionization mass spectrometry. On the basis of assay results, heartworm infection was detected in 13 of 13 antigen-positive dogs of unknown microfilaria status, 11 of 11 antigen-positive dogs with circulating microfilaria, 0 of 3 antigen-positive dogs tested at 3 months after larval infection, 0 of 2 antigen-positive dogs with occult infections, and 0 of 10 uninfected dogs. With the assay under investigation, it was possible to identify D immitis infection in dogs with circulating microfilaria via detection of the obligate Wolbachia endosymbiont of D immitis. It was not possible to identify dogs with occult infections, which suggested that circulating microfilaria must be present to detect infection with this assay, although further studies would be required to verify that finding.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="58685980"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="58685980"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 58685980; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=58685980]").text(description); $(".js-view-count[data-work-id=58685980]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 58685980; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='58685980']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 58685980, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=58685980]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":58685980,"title":"Detection of heartworm infection in dogs via PCR amplification and electrospray ionization mass spectrometry of nucleic acid extracts from whole blood samples","translated_title":"","metadata":{"abstract":"To develop and evaluate a rapid and accurate assay involving PCR amplification and electrospray ionization mass spectrometry of nucleic acid extracts from whole blood samples for the detection of Dirofilaria immitis infection in dogs. Whole blood nucleic acid extracts from 29 dogs experimentally infected with D immitis (and in which circulating D immitis antigen was detected) and 10 uninfected dogs. 16 of the 29 whole blood samples from infected dogs were examined at the time of collection for circulating microfilaria. Nucleic acids were extracted from all whole blood specimens and underwent PCR amplification with 12 PCR primer pairs designed to detect a wide range of pathogens (including the Wolbachia endosymbiont of D immitis) and electrospray ionization mass spectrometry. On the basis of assay results, heartworm infection was detected in 13 of 13 antigen-positive dogs of unknown microfilaria status, 11 of 11 antigen-positive dogs with circulating microfilaria, 0 of 3 antigen-positive dogs tested at 3 months after larval infection, 0 of 2 antigen-positive dogs with occult infections, and 0 of 10 uninfected dogs. With the assay under investigation, it was possible to identify D immitis infection in dogs with circulating microfilaria via detection of the obligate Wolbachia endosymbiont of D immitis. It was not possible to identify dogs with occult infections, which suggested that circulating microfilaria must be present to detect infection with this assay, although further studies would be required to verify that finding.","publisher":"American Veterinary Medical Association (AVMA)","publication_date":{"day":null,"month":null,"year":2012,"errors":{}},"publication_name":"American Journal of Veterinary Research"},"translated_abstract":"To develop and evaluate a rapid and accurate assay involving PCR amplification and electrospray ionization mass spectrometry of nucleic acid extracts from whole blood samples for the detection of Dirofilaria immitis infection in dogs. Whole blood nucleic acid extracts from 29 dogs experimentally infected with D immitis (and in which circulating D immitis antigen was detected) and 10 uninfected dogs. 16 of the 29 whole blood samples from infected dogs were examined at the time of collection for circulating microfilaria. Nucleic acids were extracted from all whole blood specimens and underwent PCR amplification with 12 PCR primer pairs designed to detect a wide range of pathogens (including the Wolbachia endosymbiont of D immitis) and electrospray ionization mass spectrometry. On the basis of assay results, heartworm infection was detected in 13 of 13 antigen-positive dogs of unknown microfilaria status, 11 of 11 antigen-positive dogs with circulating microfilaria, 0 of 3 antigen-positive dogs tested at 3 months after larval infection, 0 of 2 antigen-positive dogs with occult infections, and 0 of 10 uninfected dogs. With the assay under investigation, it was possible to identify D immitis infection in dogs with circulating microfilaria via detection of the obligate Wolbachia endosymbiont of D immitis. It was not possible to identify dogs with occult infections, which suggested that circulating microfilaria must be present to detect infection with this assay, although further studies would be required to verify that finding.","internal_url":"https://www.academia.edu/58685980/Detection_of_heartworm_infection_in_dogs_via_PCR_amplification_and_electrospray_ionization_mass_spectrometry_of_nucleic_acid_extracts_from_whole_blood_samples","translated_internal_url":"","created_at":"2021-10-17T19:34:31.090-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":25381401,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Detection_of_heartworm_infection_in_dogs_via_PCR_amplification_and_electrospray_ionization_mass_spectrometry_of_nucleic_acid_extracts_from_whole_blood_samples","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":25381401,"first_name":"Ranga","middle_initials":null,"last_name":"Sampath","page_name":"RangaSampath","domain_name":"independent","created_at":"2015-01-26T21:28:55.724-08:00","display_name":"Ranga Sampath","url":"https://independent.academia.edu/RangaSampath"},"attachments":[],"research_interests":[{"id":5769,"name":"Mass Spectrometry","url":"https://www.academia.edu/Documents/in/Mass_Spectrometry"},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences"},{"id":52438,"name":"Dogs","url":"https://www.academia.edu/Documents/in/Dogs"},{"id":99234,"name":"Animals","url":"https://www.academia.edu/Documents/in/Animals"},{"id":118339,"name":"Polymerase Chain Reaction","url":"https://www.academia.edu/Documents/in/Polymerase_Chain_Reaction"},{"id":178906,"name":"Nucleic Acids","url":"https://www.academia.edu/Documents/in/Nucleic_Acids"},{"id":809882,"name":"Base Sequence","url":"https://www.academia.edu/Documents/in/Base_Sequence"},{"id":973347,"name":"Ribosomal DNA","url":"https://www.academia.edu/Documents/in/Ribosomal_DNA"},{"id":2467566,"name":"Molecular Sequence Data","url":"https://www.academia.edu/Documents/in/Molecular_Sequence_Data"},{"id":3067128,"name":"reverse transcriptase polymerase chain reaction","url":"https://www.academia.edu/Documents/in/reverse_transcriptase_polymerase_chain_reaction"},{"id":3253374,"name":"Dirofilariasis","url":"https://www.academia.edu/Documents/in/Dirofilariasis"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="58685915"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/58685915/Usefulness_of_multilocus_polymerase_chain_reaction_followed_by_electrospray_ionization_mass_spectrometry_to_identify_a_diverse_panel_of_bacterial_isolates"><img alt="Research paper thumbnail of Usefulness of multilocus polymerase chain reaction followed by electrospray ionization mass spectrometry to identify a diverse panel of bacterial isolates" class="work-thumbnail" src="https://attachments.academia-assets.com/72976716/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/58685915/Usefulness_of_multilocus_polymerase_chain_reaction_followed_by_electrospray_ionization_mass_spectrometry_to_identify_a_diverse_panel_of_bacterial_isolates">Usefulness of multilocus polymerase chain reaction followed by electrospray ionization mass spectrometry to identify a diverse panel of bacterial isolates</a></div><div class="wp-workCard_item"><span>Diagnostic microbiology and infectious disease</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Polymerase chain reaction electrospray ionization mass spectrometry (PCR/ESI-MS) was tested for i...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Polymerase chain reaction electrospray ionization mass spectrometry (PCR/ESI-MS) was tested for its ability to accurately identify a blinded panel of 156 diverse bacterial isolates, mostly human and/or animal pathogens. Here, 142/156 (91%) isolates were correctly identified to the genus level and 115/156 (74%) were correctly identified to the species level. Only 9% were misidentified. This study shows that multilocus PCR/ESI-MS has the potential to be a useful technique for identifying a broad range of bacteria.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="e66f1738f5bb2068c2cfb1a44acff25e" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":72976716,"asset_id":58685915,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/72976716/download_file?st=MTczMjQyMjk1Myw4LjIyMi4yMDguMTQ2&st=MTczMjQyMjk1Myw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="58685915"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="58685915"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 58685915; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=58685915]").text(description); $(".js-view-count[data-work-id=58685915]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 58685915; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='58685915']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 58685915, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "e66f1738f5bb2068c2cfb1a44acff25e" } } $('.js-work-strip[data-work-id=58685915]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":58685915,"title":"Usefulness of multilocus polymerase chain reaction followed by electrospray ionization mass spectrometry to identify a diverse panel of bacterial isolates","translated_title":"","metadata":{"abstract":"Polymerase chain reaction electrospray ionization mass spectrometry (PCR/ESI-MS) was tested for its ability to accurately identify a blinded panel of 156 diverse bacterial isolates, mostly human and/or animal pathogens. 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