GENEWIZ from Azenta | SNP Genotyping

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href='/en/Public/Services/Next-Generation-Sequencing/Standalone-NGS-Solutions' style='color:#585F69;font-size:14px'>Sequencing Only<br></a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Whole-Genome-Sequencing' style='color:#585F69;font-size:14px'>Whole Genome Sequencing</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/High-Throughput-Genotyping' style='color:#585F69;font-size:14px'>CRISPR Validation</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Targeted-Resequencing-Panels' style='color:#585F69;font-size:14px'>Targeted Sequencing</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Metagenomics-Solutions' style='color:#585F69;font-size:14px'>Metagenomics </a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Epigenomics' style='color:#585F69;font-size:14px'>Epigenomics</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Antibody-Discovery' style='color:#585F69;font-size:14px'>Antibody Discovery <sup style="color:#826ec3; font-weight: bold;">NEW</sup></a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Immunogenomics' style='color:#585F69;font-size:14px'>Immunogenomics</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Digital-Spatial-Profiling' style='color:#585F69;font-size:14px'>Digital Spatial Profiling</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/AAV-Genome-Sequencing' style='color:#585F69;font-size:14px'>AAV Genome Sequencing</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Whole-Plasmid-Sequencing-Plasmid-EZ' style='color:#585F69;font-size:14px'>Whole Plasmid Sequencing: Plasmid-EZ <sup style="color:#826ec3; font-weight: bold;">NEW</sup></a></li></ul></div><div class='col-md-3 '><ul class='list-unstyled'><li><a class='GColor' href='/en/Public/Services/Sanger-Sequencing' style='color:#826ec3;font-size:17px'>Sanger Sequencing</a></li><li><a href='/en/Public/Services/Sanger-Sequencing/Sanger-EZ' style='color:#585F69;font-size:14px'>Sanger-EZ <sup style="color:#826ec3; font-weight: bold;">NEW</sup></a></li><li><a href='/en/Public/Services/Sanger-Sequencing/AAV-ITR' style='color:#585F69;font-size:14px'>AAV-ITR Sanger Sequencing</a></li><li><a href='/en/Public/Services/Sanger-Sequencing/Purified-Templates' style='color:#585F69;font-size:14px'>Purified Templates</a></li><li><a href='/en/Public/Services/Sanger-Sequencing/Difficult-Template-Sequencing' style='color:#585F69;font-size:14px'>Difficult Template Sequencing</a></li><li><a href='/en/Public/Services/Sanger-Sequencing/Direct-Colony-Sequencing' style='color:#585F69;font-size:14px'>Direct Colony Sequencing</a></li><li><a href='/en/Public/Services/Sanger-Sequencing/PCR-Purification' style='color:#585F69;font-size:14px'>PCR Purification</a></li><li><a href='/en/Public/Services/Sanger-Sequencing/Primer-Walking' style='color:#585F69;font-size:14px'>Primer Walking</a></li><br><li><a class='GColor' href='/en/Public/Services/Molecular-Genetics' style='color:#826ec3;font-size:17px'>PCR + Sanger Services</a></li><li><a href='/en/Public/Services/Molecular-Genetics/Bacterial-and-Fungal-Identification' style='color:#585F69;font-size:14px'>Bacterial and Fungal Identification</a></li><li><a href='/en/Public/Services/Molecular-Genetics/Mutation-Analysis' style='color:#585F69;font-size:14px'>Mutation Analysis</a></li><li><a href='/en/Public/Services/Molecular-Genetics/SNP-Genotyping' style='color:#585F69;font-size:14px'>SNP Genotyping</a></li><li><a href='/en/Public/Services/Molecular-Genetics/PCR-Plus-Sequencing-CRISPR-Analysis-Package' style='color:#585F69;font-size:14px'>PCR Plus Sequencing CRISPR Analysis Package</a></li><li><a href='/en/Public/Services/Molecular-Genetics/cDNA-Verification' style='color:#585F69;font-size:14px'>Confirmatory Sequencing & Cell Bank Characterization</a></li><li><a href='/en/Public/Services/Molecular-Genetics/Yeast-Colony-Sequencing' style='color:#585F69;font-size:14px'>Yeast Colony Sequencing</a></li><li><a href='/en/Public/Services/Molecular-Genetics/Fragment-Analysis' style='color:#585F69;font-size:14px'>Fragment Analysis</a></li><li><a href='/en/Public/Services/Molecular-Genetics/PCR-Solutions' style='color:#585F69;font-size:14px'>Quantitative PCR & Digital PCR <sup style="color:#826ec3;"><strong>NEW </strong></sup></a></li><li><a href='/en/Public/Services/Molecular-Genetics/Nucleic-Acid-Extraction' style='color:#585F69;font-size:14px'>Nucleic Acid Extraction</a></li></ul></div><div class='col-md-3 '><ul class='list-unstyled'><li><a class='GColor' href='/en/Public/Services/Gene-Synthesis' style='color:#826ec3;font-size:17px'>DNA & RNA Synthesis</a></li><li><a href='/en/Public/Services/Gene-Synthesis/Gene-Synthesis' style='color:#585F69;font-size:14px'>Gene Synthesis</a></li><li><a href='/en/Public/Services/Gene-Synthesis/Antibody-DNA-Synthesis' style='color:#585F69;font-size:14px'>Antibody Synthesis</a></li><li><a href='/en/Public/Services/Gene-Synthesis/AAV-Plasmid-Synthesis' style='color:#585F69;font-size:14px'>AAV Plasmid Synthesis</a></li><li><a href='/en/Public/Services/Gene-Synthesis/Gene-Fragments' style='color:#585F69;font-size:14px'>Gene Fragments</a></li><li><a href='/en/Public/Services/Gene-Synthesis/CRISPR-Construct-Synthesis' style='color:#585F69;font-size:14px'>CRISPR Construct Synthesis</a></li><li><a href='/en/Public/Services/Gene-Synthesis/Synthetic-DNA-Libraries' style='color:#585F69;font-size:14px'>Synthetic DNA Libraries</a></li><li><a href='/en/Public/Services/Gene-Synthesis/Single-Stranded-DNA-Synthesis' style='color:#585F69;font-size:14px'>ssDNA Synthesis</a></li><li><a href='/en/Public/Services/Gene-Synthesis/DNA-Cloning' style='color:#585F69;font-size:14px'>DNA Cloning</a></li><li><a href='/en/Public/Services/Gene-Synthesis/Site-Directed-Mutagenesis' style='color:#585F69;font-size:14px'>Site-Directed Mutagenesis<br></a></li><li><a href='/en/Public/Services/Gene-Synthesis/Viral-Package' style='color:#585F69;font-size:14px'>Viral Packaging <sup style="color:#826ec3;"><strong>NEW </strong></sup></a></li><li><a href='/en/Public/Services/Gene-Synthesis/Recombinant-Antibody-Production' style='color:#585F69;font-size:14px'>Recombinant Antibody Production <sup style="color:#826ec3;"><strong>NEW </strong></sup></a></li><li><a href='/en/Public/Services/Gene-Synthesis/Custom-mRNA-Synthesis' style='color:#585F69;font-size:14px'>Custom mRNA Synthesis <sup style="color:#826ec3; font-weight: bold;">NEW</sup></a></li><br><li><a class='GColor' href='/en/Public/Services/Oligo' style='color:#826ec3;font-size:17px'>Oligo Synthesis Services</a></li><li><a href='/en/Public/Services/Oligo/Overnight-DNA-Oligos' style='color:#585F69;font-size:14px'>Overnight DNA Oligos</a></li><li><a href='/en/Public/Services/Oligo/Custom-DNA-and-RNA-Oligos' style='color:#585F69;font-size:14px'>Custom DNA and RNA Oligos</a></li><li><a href='/en/Public/Services/Oligo/Oligo-Retained-for-Sanger-Sequencing' style='color:#585F69;font-size:14px'>Oligo Retained for Sanger Sequencing</a></li></ul></div><div class='col-md-3 '><ul class='list-unstyled'><li><a class='GColor' href='/en/Public/Services/Plasmid-DNA-Prep' style='color:#826ec3;font-size:17px'>Plasmid DNA Preparation</a></li><li><a href='/en/Public/Services/Plasmid-DNA-Prep/Standard' style='color:#585F69;font-size:14px'>Standard Plasmid DNA Prep</a></li><li><a href='/en/Public/Services/Plasmid-DNA-Prep/Targeted-Yield' style='color:#585F69;font-size:14px'>Targeted Yield Plasmid DNA Prep</a></li><li><a href='/en/Public/Services/Plasmid-DNA-Prep/AAV-Plasmid-Preparation' style='color:#585F69;font-size:14px'>AAV Plasmid Preparation</a></li><br><li><a class='GColor' href='/en/Public/Services/Clinical-Services' style='color:#826ec3;font-size:17px'>PreClinical and Clinical Services</a></li><li><a href='/en/Public/Services/Clinical-Services/Clinical-Services-Overview' style='color:#585F69;font-size:14px'>Clinical Services</a></li><li><a href='/en/Public/Services/Clinical-Services/Microarray-Solutions' style='color:#585F69;font-size:14px'>Microarray Solutions <sup style="color:#826ec3; font-weight: bold;">NEW</sup></a></li><li><a href='/en/Public/Services/Clinical-Services/CLIA-Variant-Confirmation' style='color:#585F69;font-size:14px'>CLIA Variant Confirmation (PCR + Sanger)</a></li><li><a href='/en/Public/Services/Clinical-Services/CLIA-Sanger-Sequencing' style='color:#585F69;font-size:14px'>CLIA Sanger Sequencing</a></li><li><a href='/en/Public/Services/Clinical-Services/CLIA-Whole-Exome-Sequencing' style='color:#585F69;font-size:14px'>CLIA Whole Exome Sequencing</a></li><li><a href='/en/Public/Services/Clinical-Services/CLIA-Whole-Genome-Sequencing' style='color:#585F69;font-size:14px'>CLIA Whole Genome Sequencing</a></li><li><a href='/en/Public/Services/Clinical-Services/Regulatory' style='color:#585F69;font-size:14px'>GLP-Compliant Overview</a></li><li><a href='/en/Public/Services/Clinical-Services/GLP-compliant-Confirmatory-Sequencing' style='color:#585F69;font-size:14px'>GLP Confirmatory Sequencing</a></li><li><a href='/en/Public/Services/Clinical-Services/GLP-compliant-SNP-Mutation-Analysis' style='color:#585F69;font-size:14px'>GLP SNP Mutation Analysis</a></li><li><a href='/en/Public/Services/Clinical-Services/GLP-compliant-Plasmid-Prep' style='color:#585F69;font-size:14px'>GLP Plasmid Prep</a></li><li><a href='/en/Public/Services/Clinical-Services/Nucleic-Acid-Extraction' style='color:#585F69;font-size:14px'>GLP Nucleic Acid Extraction</a></li><li><a href='/en/Public/Services/Clinical-Services/Biofluid-Processing-Overview' style='color:#585F69;font-size:14px'>Biofluid Processing</a></li><li><a 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This method identifies genetic variations by comparing an individual’s sequence against a wild-type reference sequence to analyze single nucleotide polymorphisms (SNPs). SNPs are present in both the coding and non-coding regions of a gene and are one of the most common types of variants; they are represented as a single nucleotide mutation within a DNA sequence.</p> <p>Azenta Life Sciences, formerly GENEWIZ’s SNP Genotyping service is a PCR and Sanger sequencing-based solution that is used to ramp up SNP screening assays and validate SNPs of interest with speed and accuracy.</p> <p> </p> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://clims4.genewiz.com/DynamicForm/CreateView?lobId=10&serviceId=1002" target="_blank"><button>Request Quote</button></a> </div> <div class="ngs-content-box" id="ngs-content-box-right"> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://www.genewiz.com/en/Public/Services/Molecular-Genetics/PCR-Solutions" target="_blank"><img alt="" src="/-/media/17001DqPCRdPCRWebBanRight2.ashx?la=en&hash=667C0B7B2E5796BDD1BF6575F2DB2C22E6168950" /></a> </div> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <link href="https://fonts.googleapis.com/css?family=Roboto:300,400,500,700" rel="stylesheet" /> <style type="text/css"> .covid-banner { width: 1170px; height: auto; margin: 0 auto; background-image: url("/-/media/11037-D_Sanger-Troubleshoot_WebBanFull-Med_Background.ashx"); background-size: cover; background-position: center; text-align: left; padding: 0px 0 0px 0; } .covid-content { text-align: center; } .covid-box h3 { color: #ffffff; text-transform: none; letter-spacing: 1px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 450; line-height: 125%; font-size: 35px; margin: 0; padding-bottom: 15px; padding-top: 25px; } .covid-box h4 { color: #ffffff; text-transform: none; letter-spacing: 1px; line-height: 30px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 35px; margin: 0; margin-bottom: 5px; } .covid-box p { color: #000000; text-transform: none; letter-spacing: 1px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 15px; margin: 0; margin-bottom: 5px; } .covid-box button { padding: 8px 19px; color: #826ec3; letter-spacing: 1px; background: #ffffff; border: 2px solid #ffffff; border-radius: 0px; transition: all .2s ease-in-out; font-size: 15px } .covid-box button:hover { cursor: pointer; transform: scale(1.1); } .covid-box button:focus { outline: 0; } @media only screen and (max-width: 1170px) { .covid-banner { width: 100%; height: auto; } .covid-content { width: 90%; margin: 0 auto; } } </style> <br /> <div class="covid-banner"> <div class="covid-content"> <div class="covid-box"> <br /> <h3>Troubleshooting DNA Templates<br /> with Sanger Sequencing</h3> <a href="https://hubs.ly/Q02BXrg90" target="_blank"> <br /> <button> READ BLOG</button> <br /> </a> <p> </p> </div> </div> </div> <p> </p> </div> <div class="headline-center Rich-Text-Edit-Area"> <div class="container"> <h2>SNP Genotyping Analysis and Detection</h2> <p style="text-align: left;"> SNP analysis and identification may help predict an individual’s response to certain drugs, susceptibility to environmental factors, and risk for developing diseases. Alternatively, SNPs can be used to track inheritance of disease genes within families and provide insight into SNP linkage. </p> <p style="text-align: left;"> Sanger sequencing-based SNP genotyping can be performed on a small subset of samples or as part of large-scale projects. Whether you are using genotyping as your primary approach for SNP analysis and detection, or to confirm next generation sequencing and microarray results, Azenta can help accelerate your project. Let the Sanger sequencing experts at Azenta support your SNP genotyping research objectives with speed, quality, and reliability.</p> </div> <br /> <br /> <br /> </div> <div class="headline-center Rich-Text-Edit-Area"> <style> .ma-btn { border: none; width: 235px; height: 55px; background: #3498db; color: #fff; letter-spacing: 1px; } </style> <div class="col-md-6 col-centered"> <h2>Service Highlights</h2> <table class="table table-responsive table-bordered"> <tbody class="panel panel-purple"> <tr class="panel-heading"> <td colspan="2">SNP GENOTYPING</td> </tr> <tr> <td>Starting Material</td> <td>gDNA (extractions available)</td> </tr> <tr> <td>Assay Coverage</td> <td>Annotated SNPs of interest</td> </tr> <tr> <td>Confirm</td> <td>SNPs</td> </tr> <tr> <td rowspan="2">Deliverables/Reports</td> <td>Raw data files: .ab1, .seq</td> </tr> <tr> <td>Nucleotide level mutations </td> </tr> </tbody> </table> <p>*Customized reports available</p>  <br /> <p>Looking to identify non-annotated mutations in coding or non-coding regions of interest? See Azenta’s <a href="https://www.genewiz.com/Public/Services/Molecular-Genetics/Mutation-Analysis">Mutation Analysis service</a>.</p> </div> </div> <script type="text/javascript" src="/assets/plugins/counter/jquery.counterup.min.js"></script> <script language="javascript" type="text/javascript"> jQuery(document).ready(function ($) { $(".row .category").find("img").addClass("img-responsive"); }); </script> <div class='Component-Frame pull-center Multi-column-TextBullet-Layout Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10'> <div class="container"> <div class="headline-center"> <h2>Features & Benefits</h2> </div> <div class="headline-left"> </div> <div class="row category"> <div class="col-md-6 col-sm-6"> <div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> <strong>Reliable Assay Development:</strong> Our highly-trained scientists develop robust and reproducible PCR assays targeted to your SNPs of interest.<br><br><br> </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> <strong>High-Throughput Processing</strong>: Target SNPs are amplified using optimized assays; amplicons are purified and efficiently sequenced by capillary electrophoresis in our high-throughput facility.<br> </div> </div> </div> <div class="col-md-6 col-sm-6"> <div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> <strong>Accurate Data Analysis:</strong> SNP are identified by software programs and confirmed by Azenta scientists to ensure accuracy of the reported data. Azenta will provide a report with nucleotide level findings. Customizable reports are available upon request for a complete start-to-finish solution.<br> </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> <strong>Developed Assay Lists:</strong> Clients can store assays with Azenta and re-order them over time from a dynamic assay list that will be built to fit each client’s research needs. <br> </div> </div> </div> </div> </div> </div><div class="headline-center Rich-Text-Edit-Area"> <link href="https://fonts.googleapis.com/css?family=Roboto:300,400,500,700" rel="stylesheet" /> <style type="text/css"> .oligo-pools-banner1 { width: 1170px; margin: 0 auto; background-image: url("/-/media/25OffPCRSangerWebBanFullMedBackground.ashx"); background-size: cover; background-position: center center; text-align: left; padding: 40px 35px; } .oligo-pools-content1 { text-align: center; } .oligo-pools-box1 h3 { color: #FFFFFF; text-transform: none; letter-spacing: 1px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 500; font-size: 40px; margin: 10px; margin-bottom: 15px; line-height: 40px } .oligo-pools-box1 span { font-family: 'Roboto', sans-serif; font-weight: 300; } #oligo-pools-box-right1 { margin-top: 30px; } #oligo-pools-box-left1 span { font-weight: 200; font-size: 14px; letter-spacing: 1px; } #oligo-pools-box-right1 button { padding: 8px 15px; color: #ffffff; letter-spacing: 1px; background: #55c8d2; border: 2px solid #55c8d2; border-radius: 0px; transition: all .2s ease-in-out; font-size: 16px } #oligo-pools-box-right1 button:hover { cursor: pointer; background: #55c8d2; border: 2px solid #55c8d2; color: #ffffff; transform: scale(1.2); } #oligo-pools-box-right1 button:focus { outline: 0; } @media only screen and (max-width: 1170px) { .oligo-pools-banner1 { width: 90%; } .oligo-pools-content1 { width:90%; margin: 0 auto; } .oligo-pools-box1 h3 { font-size: 40px; } .oligo-pools-box1 p { line-height: 25px } } </style> <div class="oligo-pools-banner1"> <div class="oligo-pools-content1"> <div class="oligo-pools-box1" id="oligo-pools-box-left1"> <h3>Get 25% off your first PCR + Sanger order</h3> <div class="oligo-pools-box1" id="oligo-pools-box-right1"> <a href="https://web.azenta.com/mgrs-25" target="blank"> <button> CLAIM PROMO </button> </a> </div> </div> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> .workflow-container { width: 76%; margin: 0 auto; margin-top: 40px; } .workflow { width: 100%; margin: 0 auto; text-align: center; } .workflow-step { display: inline-block; width: 22%; vertical-align: top; padding: 5px; margin-bottom: 40px; } .workflow-step img { margin: 0 auto; margin-bottom: 40px; } .workflow-step h3 { border-bottom: none; } .workflow-step p { } .workflow-icon { text-align: center; } @media only screen and (max-width: 1200px) { .workflow { text-align: left; margin: 0 auto; } .workflow-step { width: 45%; } } @media only screen and (max-width: 500px) { .workflow-step { width: 100%; } } </style> <h3 style="text-align: center; margin-bottom: 30px;">AZENTA SNP GENOTYPING WORKFLOW</h3> <div class="workflow-container"> <div class="workflow"> <div class="workflow-step"> <div class="workflow-icon"> <img alt="" src="/-/media/Images/Services/MG/icons/210x110/ma-data-generalization-grey.ashx?la=en&hash=62D6DE3113A7C2226A88C5F4F316CD04806FECCD" /> </div> <h3>1. PROJECT CONSULTATION & PRIMER DESIGN</h3> <p>Amplicon selection and primer design to targeted SNPs of interest</p> </div> <div class="workflow-step"> <div class="workflow-icon"> <img alt="" src="/-/media/Images/Services/NGS/Workflows/library-prep-workflow_110x210.ashx?la=en&hash=517F7A2E145D39BF36DDADC48A68DFE054DCD56A" /> </div> <h3>2. ASSAY DEVELOPMENT<br /> <br /> </h3> <p>Optimization of project-specific samples</p> </div> <div class="workflow-step"> <div class="workflow-icon"> <img alt="" src="/-/media/Images/Services/NGS/icons/210x110/PCR-Primer-DesignOptimization_110x210.ashx?la=en&hash=65467A3FC5D795A6B9382DC795266E874DBDBCC0" /> </div> <h3>3. PCR & PURIFICATION<br /> <br /> </h3> <p>PCR amplification of genomic DNA using optimized conditions</p> </div> <div class="workflow-step"> <div class="workflow-icon"> <img alt="" src="/-/media/Images/Services/MG/icons/210x110/ma-sequencing-data-mg-grey.ashx?la=en&hash=93127C85C9AB576D3DCD98C93242D7ECEEF7A97F" /> </div> <h3>4. SEQUENCING & DATA ANALYSIS<br /> <br /> </h3> <p>Bi-directional DNA sequencing per amplicon and final report identifying SNPs</p> </div> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <div class="container"> <h2>Azenta <span style="text-transform: none;">vs. In-House</span></h2> <p>SNP Genotyping Turnaround Time Comparison: Azenta versus In-house</p> <h3>AZENTA TURNAROUND: 3 DAYS</h3> <img alt="" class="img-responsive" src="/-/media/Images/Services/MG/3days.ashx?la=en&hash=736C4A4140914A524209FB328685AF0FD4DBE2DF" width="100%"> <h3>IN-HOUSE TURNAROUND: 2-4 WEEKS</h3> <img alt="" class="img-responsive" src="/-/media/Images/Services/MG/workflow/inhouse-turnaround-2-4-weeks.ashx?la=en&hash=D3874AEE92C397F343940D0F12D812807825F3AB"> </div> <br> <br> </div> <div class="headline-center Rich-Text-Edit-Area"> <div class="container" style="text-align: left;"> <h2 style="text-align: center;">Deliverables</h2> <p>All SNP Genotyping projects will receive a raw data file (Figure 1.) along with a final report identifying SNPs compared to the provided reference sequence (Figure 2.). Custom reports are also available upon request.</p> <strong></strong> <p><strong>Example: BRAF c.1799T>A (V600E) Mutation in Melanoma</strong></p> <p>Mutations in BRAF are largely linked to malignant melanomas. Mutations at BRAF V600 are common indicators for responsiveness to treatment for melanoma patients.<br /> <br /> <strong>Figure 1. Chromatogram</strong><br /> Chromatogram showing the heterozygous mutation at nucleotide position 1799 (T to A mutation at position 16 below) which correlates to amino acid position 600 (V to E mutation).<br /> <img alt="" src="-/media/834C45BBA4914D5C91858850B56EF781.ashx" /><img alt="" src="/-/media/Images/Services/MG/photos/chromatogram.ashx?la=en&hash=C646FDA0EE2E2B3FB958946E703964DA9BBCC412"/></p> <p> <br /> <strong>Figure 2. Final Report<br /> </strong></p> <p>A SNP genotyping final report shows the reference SNP ID number, the allelic mutation, and the flanking sequence with the highlighted position of the identified SNP.</p> <table class="table table-responsive table-bordered"> <tbody class="panel panel-purple"> <tr class="panel-heading"> <td colspan="2">SNP ID</td> <td>Alelles</td> <td>Flanking Sequence</td> </tr> <tr> <td colspan="2">rs113488022</td> <td>T/A</td> <td>TTGGTCTAGCTACAG<span style="color: #ff0000;">T</span>GAAATCTCGATGGAG</td> </tr> </tbody> </table> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> .related-services-container { width: 76%; margin: 0 auto; margin-top: 40px; } .related-services { width: 100%; margin: 0 auto; text-align: center; } .related-services-block { display: inline-block; width: 18%; vertical-align: top; padding: 5px; margin-bottom: 40px; } .related-services-block img { width: 150px; margin: 0 auto; margin-bottom: 40px; } .related-services-block h3 { text-align: center; font-size: 18px; font-family: sans-serif; border-bottom: none; } .related-services-block p { width: 80%; margin: 0 auto; text-align: left; padding: 0; } .related-services-icon { text-align: center; } @media only screen and (max-width: 1200px) { .related-services { text-align: left; margin: 0 auto; } .related-services-block { width: 45%; } } @media only screen and (max-width: 500px) { .related-services-block { width: 100%; } } </style> <h2 style="text-align: center; margin-bottom: 30px;">Related Services</h2> <div class="related-services-container"> <div class="related-services"> <div class="related-services-block"> <div class="related-services-icon"> <a href="https://www.genewiz.com/en/Public/Services/Molecular-Genetics/cDNA-Verification"><img alt="" src="/-/media/Images/Home/icons/Purple-Icons/cDNA-verification-services_110x210_Purple.ashx?la=en&hash=115D2256CBBFC3BE64778CC4B92F41CA84406FBA" /></a> </div> <h3>RNA/cDNA Verification<br /> <br /> </h3> <p>Verify your cell bank sequences at either the cDNA or gDNA level</p> </div> <div class="related-services-block"> <div class="related-services-icon"> <a href="https://www.genewiz.com/Public/Services/Molecular-Genetics/PCR-Plus-Sequencing-CRISPR-Analysis-Package"><img alt="" src="/-/media/Images/Home/icons/Purple-Icons/PCR-PLUS-SEQUENCING-CRISPR-ANALYSIS-icon_210x110_Purple_DESIGN.ashx?la=en&hash=14E33A6152179BC267AC8AABF371834369DF33AD" /></a> </div> <h3>CRISPR Analysis Package<br /> <br /> </h3> <p>Determine the efficiency of CRISPR-mediated genome editing with this Sanger based service. TA cloning options available.</p> </div> <div class="related-services-block"> <div class="related-services-icon"> <a href="https://www.genewiz.com/Public/Services/Molecular-Genetics/Mutation-Analysis"><img alt="" src="/-/media/Images/Home/icons/Purple-Icons/Mutation-Analysis_110x210_Purple.ashx?la=en&hash=FF8FD0CA5F8A6C78F02332D0C16CAC1C02CB539C" /></a> </div> <h3>Mutation Analysis<br /> <br /> </h3> <p>PCR and Sequencing-based mutation analysis service used to detect mutations in coding exons.</p> </div> <div class="related-services-block"> <div class="related-services-icon"> <a href="https://www.genewiz.com/Public/Services/Next-Generation-Sequencing/genoTYPER-NEXT"><img alt="" src="/-/media/Images/Home/icons/Purple-Icons/genotyper-210x110_Purple.ashx?la=en&hash=366518EE7A3F476BDE503CBFD8BDBE5CE771F490" /></a> </div> <h3>genoTYPER-NEXT<br /> <br /> </h3> <p>High-throughput, ultra-sensitive, NGS genotyping solution used to identify on-target mutations</p> </div> <div class="related-services-block"> <div class="related-services-icon"> <a href="https://www.genewiz.com/Public/Services/Regulatory/GLP-compliant-SNP-Mutation-Analysis"><img alt="" src="/-/media/Images/Home/icons/Purple-Icons/snp-genoytping-icon_Purple.ashx?la=en&hash=C9C1A73326B9252E4EE657A5C642F0A047719702" /></a> </div> <h3>GLP-Compliant SNP/Mutation Analysis<br /> <br /> </h3> <p>Support your FDA and EPA submissions by identifying SNPs or mutations with GLP-compliant sequencing. </p> </div> </div> </div> </div>  <div class="process-v1 How-To-Order Component-BackGround-Gray margin-top-0 padding-top-10 padding-bottom-10 "> <div class=" container"> <div class="headline-center"> <div class="row"> <h2>HOW TO ORDER</h2> </div> <div class="row"> *Samples must arrive at the Azenta New Jersey Laboratory before 10:00 am EST to qualify for Same Day service. 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