CLIA Sanger Sequencing | GENEWIZ from Azenta

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Walking</a></li><br><li><a class='GColor' href='/en/Public/Services/Molecular-Genetics' style='color:#826ec3;font-size:17px'>PCR + Sanger Services</a></li><li><a href='/en/Public/Services/Molecular-Genetics/Bacterial-and-Fungal-Identification' style='color:#585F69;font-size:14px'>Bacterial and Fungal Identification</a></li><li><a href='/en/Public/Services/Molecular-Genetics/Mutation-Analysis' style='color:#585F69;font-size:14px'>Mutation Analysis</a></li><li><a href='/en/Public/Services/Molecular-Genetics/SNP-Genotyping' style='color:#585F69;font-size:14px'>SNP Genotyping</a></li><li><a href='/en/Public/Services/Molecular-Genetics/PCR-Plus-Sequencing-CRISPR-Analysis-Package' style='color:#585F69;font-size:14px'>PCR Plus Sequencing CRISPR Analysis Package</a></li><li><a href='/en/Public/Services/Molecular-Genetics/cDNA-Verification' style='color:#585F69;font-size:14px'>Confirmatory Sequencing & Cell Bank Characterization</a></li><li><a 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href='/en/Public/Services/Gene-Synthesis/Antibody-DNA-Synthesis' style='color:#585F69;font-size:14px'>Antibody Synthesis</a></li><li><a href='/en/Public/Services/Gene-Synthesis/AAV-Plasmid-Synthesis' style='color:#585F69;font-size:14px'>AAV Plasmid Synthesis</a></li><li><a href='/en/Public/Services/Gene-Synthesis/Gene-Fragments' style='color:#585F69;font-size:14px'>Gene Fragments</a></li><li><a href='/en/Public/Services/Gene-Synthesis/CRISPR-Construct-Synthesis' style='color:#585F69;font-size:14px'>CRISPR Construct Synthesis</a></li><li><a href='/en/Public/Services/Gene-Synthesis/Synthetic-DNA-Libraries' style='color:#585F69;font-size:14px'>Synthetic DNA Libraries</a></li><li><a href='/en/Public/Services/Gene-Synthesis/Single-Stranded-DNA-Synthesis' style='color:#585F69;font-size:14px'>ssDNA Synthesis</a></li><li><a href='/en/Public/Services/Gene-Synthesis/DNA-Cloning' style='color:#585F69;font-size:14px'>DNA Cloning</a></li><li><a href='/en/Public/Services/Gene-Synthesis/Site-Directed-Mutagenesis' style='color:#585F69;font-size:14px'>Site-Directed Mutagenesis<br></a></li><li><a href='/en/Public/Services/Gene-Synthesis/Viral-Package' style='color:#585F69;font-size:14px'>Viral Packaging <sup style="color:#826ec3;"><strong>NEW </strong></sup></a></li><li><a href='/en/Public/Services/Gene-Synthesis/Recombinant-Antibody-Production' style='color:#585F69;font-size:14px'>Recombinant Antibody Production <sup style="color:#826ec3;"><strong>NEW </strong></sup></a></li><li><a href='/en/Public/Services/Gene-Synthesis/Custom-mRNA-Synthesis' style='color:#585F69;font-size:14px'>Custom mRNA Synthesis <sup style="color:#826ec3; font-weight: bold;">NEW</sup></a></li><br><li><a class='GColor' href='/en/Public/Services/Oligo' style='color:#826ec3;font-size:17px'>Oligo Synthesis Services</a></li><li><a href='/en/Public/Services/Oligo/Overnight-DNA-Oligos' style='color:#585F69;font-size:14px'>Overnight DNA Oligos</a></li><li><a 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Whole Genome Sequencing</a></li><li><a href='/en/Public/Services/Clinical-Services/Regulatory' style='color:#585F69;font-size:14px'>GLP-Compliant Overview</a></li><li><a href='/en/Public/Services/Clinical-Services/GLP-compliant-Confirmatory-Sequencing' style='color:#585F69;font-size:14px'>GLP Confirmatory Sequencing</a></li><li><a href='/en/Public/Services/Clinical-Services/GLP-compliant-SNP-Mutation-Analysis' style='color:#585F69;font-size:14px'>GLP SNP Mutation Analysis</a></li><li><a href='/en/Public/Services/Clinical-Services/GLP-compliant-Plasmid-Prep' style='color:#585F69;font-size:14px'>GLP Plasmid Prep</a></li><li><a href='/en/Public/Services/Clinical-Services/Nucleic-Acid-Extraction' style='color:#585F69;font-size:14px'>GLP Nucleic Acid Extraction</a></li><li><a href='/en/Public/Services/Clinical-Services/Biofluid-Processing-Overview' style='color:#585F69;font-size:14px'>Biofluid Processing</a></li><li><a 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from our state-of-the-art CLIA-certified and CAP-accredited laboratory while following Good Clinical Practices (GCP) guidelines. This standalone sequencing service utilizes a CLIA-compliant workflow to support your clinical diagnostic assays, leading to advancements in personalized medicine and molecular diagnostics.</p> <p>CLIA Sanger sequencing is best utilized as the initial readout from a PCR assay or in conjunction with next generation sequencing, specifically to elucidate complex regions not approachable by other methodologies or to confirm variants uncovered by genome-wide techniques.</p> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://clims4.genewiz.com/RegulatoryForm/CLIASangerSequencing?lobId=9&serviceId=909" target="_blank"><button>Place Order</button></a> </div> <div class="gs-content-box" id="gs-content-box-right"> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://hubs.ly/Q02l4sdY0" target="_blank"><img alt="" src="/-/media/10013-D_Customer-Referral_WebBanRight2.ashx?la=en&hash=21BD9C4C09D7AFE28645FBB92782B71F584DE6BE" /></a> </div> </div> </div> </div> <script type="text/javascript" src="/assets/plugins/counter/jquery.counterup.min.js"></script> <script language="javascript" type="text/javascript"> jQuery(document).ready(function ($) { $(".row .category").find("img").addClass("img-responsive"); }); </script> <div class='Component-Frame pull-center Multi-column-TextBullet-Layout Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10'> <div class="container"> <div class="headline-center"> <h2 style="border: none;">Features And Benefits</h2> </div> <div class="headline-left"> </div> <div class="row category"> <div class="col-md-6 col-sm-6"> <div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> <b style="color:#000;">Long Sequencing Reads</b>: DNA sequencing read lengths up to ~1000 bases<br><br> </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> <strong style="color:#000;">High Quality Data</strong>: Typical read will provide 800 bases Phred20 </div> </div> </div> <div class="col-md-6 col-sm-6"> <div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> <strong style="color:#000;">Fast Turnaround Time</strong>: Results available within two to three business days </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> <strong style="color:#000;">Proprietary Protocols</strong>: Best-in-class protocols to sequence difficult RNAi and GC-rich templates </div> </div> </div> </div> </div> </div><div class="headline-center Rich-Text-Edit-Area"> <link href="https://fonts.googleapis.com/css?family=Roboto:300,400,500,700" rel="stylesheet" /> <style type="text/css"> .covid-banner { width: 1170px; height: auto; margin: 0 auto; background-image: url("/-/media/16015-D_MicroarraySolutions_WebBanFull-Med_Background.ashx"); background-size: cover; background-position: center; text-align: left; padding: 0px 0 0px 0; } .covid-content { text-align: center; } .covid-box h3 { color: #ffffff; text-transform: none; letter-spacing: 1px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 450; line-height: 125%; font-size: 35px; margin: 0; padding-bottom: 15px; padding-top: 5px; } .covid-box h4 { color: #ffffff; text-transform: none; letter-spacing: 1px; line-height: 30px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 20px; margin: 0; margin-bottom: 5px; } .covid-box p { color: #ffffff; text-transform: none; letter-spacing: 1px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 15px; margin: 0; margin-bottom: 5px; } .covid-box button { padding: 8px 19px; color: #826ec3; letter-spacing: 1px; background: #ffffff; border: 2px solid #ffffff; border-radius: 0px; transition: all .2s ease-in-out; font-size: 15px } .covid-box button:hover { cursor: pointer; transform: scale(1.1); } .covid-box button:focus { outline: 0; } @media only screen and (max-width: 1170px) { .covid-banner { width: 100%; height: auto; } .covid-content { width: 90%; margin: 0 auto; } } </style> <br /> <div class="covid-banner"> <div class="covid-content"> <div class="covid-box"> <br /> <br /> <h4>Analyze thousands of genetic markers at once</h4> <h3>Microarray Solutions</h3> <a href="https://hubs.ly/Q02BX9yC0" target="_blank"> <br /> <button> LEARN MORE</button> <br /> </a> <p> </p> </div> </div> </div> <p> </p> </div> <div class="headline-center Rich-Text-Edit-Area"> <link href="https://fonts.googleapis.com/css?family=Roboto:300,400,500" rel="stylesheet" /> <style type="text/css"> .services-container { width: 90%; margin: 0 auto; text-align: center; } .services-container h2 { border: none; } .services-container p { font-size: 16px; font-weight: 300; letter-spacing: 0.4px; line-height: 22px; } .services-container a { color:#000000; text-decoration: none; transition: ease-in-out 0.2s; } .services-container a:hover { border-bottom: 2px solid #FFA500; } .services-description { display: inline-block; vertical-align: top; width: 26%;' margin: 0 auto; padding: 25px; font-family: 'Roboto', sans-serif; } .services-description img { width: 40%; } .services-description h3 { font-size: 20px; font-weight: 400; letter-spacing: 0.4px; text-align: center; border: none; } .services-description p { font-size: 16px; font-weight: 300; letter-spacing: 0.4px; line-height: 22px; text-align: left; } @media only screen and (max-width: 991px) { .services-description { width: 80%; margin: 0 auto; text-align: center; } } </style> <div> <div class="services-container"> <h2> CLIA Sanger Sequencing Service Options</h2> <div class="services-description"> <img alt="" src="/-/media/Images/Services/GA/icons/375x561/premixed-purifed-templates_375x561.ashx?la=en&hash=F2CF2A5EC2DBF227277546DD69076C64A8AC35B9" /> <h3>Pre-Mixed</h3> <p>For sequencing plasmids or purified PCR products, provide your template and primer mixed in the same tube for the most economical CLIA-compliant services.</p> </div> <div class="services-description"> <img alt="" src="/-/media/Images/Services/GA/icons/375x561/predefined-purifed-templates_375x561.ashx?la=en&hash=FCCB0AF186D96FCF7912A9D00ADD6132418B361F" /> <h3>Pre-Defined</h3> <p>If choosing our Pre-Defined Service, submit your plasmids or purified PCR products and primer in separate tubes, and a Azenta scientist will mix them once your sample arrives on site.</p> </div> <div class="services-description"> <img alt="" src="/-/media/Images/Services/GA/icons/375x561/custom-purifed-templates_375x561.ashx?la=en&hash=CD34AB43DDC68761A4954AF36FD8F7269D6E9A57" /> <h3>Custom</h3> <p>Full-service option allows you to submit unpurified PCR product with unknown concentration along with your primer for sequencing. We will purify your PCR product as well as measure and adjust the concentration to optimize your reactions.</p> </div>  </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> body { margin: 0; } .ssg-container { background-color: #f1f0f0; padding: 40px 0; text-align: center; } .ssg-container h1 { margin: 0; border: none; } .ssg-container button { border: 2px solid #826ec3; border-radius: 7px; background: #f1f0f0; margin-top: 20px; padding: 10px 20px; color: #826ec3; font-size: 16px; letter-spacing: 0.7px; cursor: pointer; transition: ease-in-out 0.2s; } .ssg-container button:hover { border:2px solid #826ec3; background: #826ec3; color: #fff; } .ssg-container button:focus { outline: 0; } </style> <div class="ssg-container"> <h1>View our Sample Submissions Guidelines</h1> <a rel="noopener noreferrer" href="https://www.genewiz.com/Public/Resources/Sample-Submission-Guidelines/Sanger-Sequencing-Sample-Submission-Guidelines/Sample-Preparation#sanger-sequence" target="_blank"><button>Learn More</button></a> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <br> <br> <link href="https://fonts.googleapis.com/css?family=Roboto:300,400,500" rel="stylesheet"> <style type="text/css"> .templates-container { width: 90%; margin: 0 auto; text-align: center; } .templates-container h2 { border: none; } .templates-title { display: inline-block; vertical-align: top; width: 25%; margin: 0 auto; padding: 25px; font-family: 'Roboto', sans-serif; } .templates-title p { font-size: 17px; font-weight: 300; letter-spacing: 0.4px; width: 70%; margin: 0 auto; margin-top: 40px; border: none; } @media only screen and (max-width: 768px) { .templates-title { width: 80%; margin: 0 auto; text-align: center; } } </style> <div> <div class="templates-container"> <h2>Template Types</h2> <div class="templates-title"> <img alt="Purified Plasmids or PCR Products" src="/-/media/Images/Services/GA/icons/210x110/CLIA/Purified-Templates.ashx?la=en&hash=73ACCF43B666FBB332050459661BFDF50BAE4740"> <p>Purified Plasmids or PCR Products</p> </div> <div class="templates-title"> <img alt="Un-Purified PCR Products" src="/-/media/Images/Services/GA/icons/210x110/CLIA/Un-Purified-PCR-grey.ashx?la=en&hash=5D1573D57732511B9184241177C100A8AAA60E82"> <p>Un-Purified PCR Products</p> </div> <div class="templates-title"> <img alt="Difficult Template Sequencing" src="/-/media/Images/Services/GA/icons/210x110/CLIA/Difficult-Template-Sequencing-grey.ashx?la=en&hash=D46DE891DD89A41D0A3E920D328914AB16A4CFDF"> <p>Difficult Template Sequencing</p> </div> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <div class="container Component-BackGround-Default"> <h2 style="border: none;">Deliverables</h2> <p>All customers will receive their raw data as .ab1, .seq, and .phd files along with a quality score report.</p> </div> <br> </div> <div class="headline-center Rich-Text-Edit-Area"> <link href="https://fonts.googleapis.com/css?family=Roboto:300,400,500,700" rel="stylesheet" /> <style type="text/css"> .covid-banner1 { width: 1170px; height: auto; margin: 0 auto; background-image: url("/-/media/11037-D_Sanger-Troubleshoot_WebBanFull-Med_Background.ashx"); background-size: cover; background-position: center; text-align: left; padding: 0px 0 0px 0; } .covid-content1 { text-align: center; } .covid-box1 h3 { color: #ffffff; text-transform: none; letter-spacing: 1px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 450; line-height: 125%; font-size: 35px; margin: 0; padding-bottom: 15px; padding-top: 25px; } .covid-box1 h4 { color: #ffffff; text-transform: none; letter-spacing: 1px; line-height: 30px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 35px; margin: 0; margin-bottom: 5px; } .covid-box1 p { color: #000000; text-transform: none; letter-spacing: 1px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 15px; margin: 0; margin-bottom: 5px; } .covid-box1 button { padding: 8px 19px; color: #826ec3; letter-spacing: 1px; background: #ffffff; border: 2px solid #ffffff; border-radius: 0px; transition: all .2s ease-in-out; font-size: 15px } .covid-box1 button:hover { cursor: pointer; transform: scale(1.1); } .covid-box1 button:focus { outline: 0; } @media only screen and (max-width: 1170px) { .covid-banner1 { width: 100%; height: auto; } .covid-content1 { width: 90%; margin: 0 auto; } } </style> <br /> <div class="covid-banner1"> <div class="covid-content1"> <div class="covid-box1"> <br /> <h3>Troubleshooting DNA Templates<br /> with Sanger Sequencing</h3> <a href="https://www.azenta.com/learning-center/blog/troubleshooting-dna-templates-sanger-sequencing?utm_campaign=2024_05_GEN_SANGER%3A%20Trouble%20Shooting%20Sanger%20Sequencing%20Blog&utm_source=EU%20CLIA%20Sanger%20Sequencing%20page&utm_medium=Full%20Banner%20Ad" target="_blank"> <br /> <button> READ BLOG</button> <br /> </a> <p> </p> </div> </div> </div> <p> </p> </div> <script language="javascript" type="text/javascript"> jQuery(document).ready(function ($) { $(".SingleIcon").find("img").addClass("img-responsive center-block"); }); </script> <div class="process-v1 icon-nav Component-BackGround-Default row-centered margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10"> <div class="container"> <div class="headline-center"> <h2 style="border: none;">Related Services</h2> <p></p> </div> <ul class="list-unstyled row "> <li class='col-sm-3 col-centered'> <a href='/Public/Services/Regulatory/GLP-compliant-Confirmatory-Sequencing' target=''> <div class='SingleIcon headline-center Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10'> <div class="row"> <img src="/-/media/Images/Home/icons/Purple-Icons/GLP-compliant-Confirmatory-Sequencing_110x210_Purple.ashx?h=110&la=en&w=210&hash=6E17481E2B90ACF9B7789BF343BD5E801683A447" alt="Building" width="210" height="110" /> </div> <div class="row"> <h3>GLP Confirmatory Sequencing</h3> </div> <div class="row"> </div> </div> </a> </li>  <li class='col-sm-3 col-centered'> <a href='/Public/Services/Sanger-Sequencing/Purified-Templates' target=''> <div class='SingleIcon headline-center Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10'> <div class="row"> <img src="/-/media/Images/Home/icons/Purple-Icons/purified-templates-110x210_Purple.ashx?h=110&la=en&w=210&hash=F8D986CF1708395031035675CB46BC51FC8A08C1" alt="Building" width="210" height="110" /> </div> <div class="row"> <h3>Sanger Sequencing<br>Purified Templates</h3> </div> <div class="row"> </div> </div> </a> </li>  <li class='col-sm-3 col-centered'> <a href='/Public/Services/Sanger-Sequencing/Difficult-Template-Sequencing' target=''> <div class='SingleIcon headline-center Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10'> <div class="row"> <img src="/-/media/Images/Home/icons/Purple-Icons/difficult-template-sequencing-110x210_Purple.ashx?h=110&la=en&w=210&hash=64970B95F8298F09414305D67166D7AE00F6D845" alt="Building" width="210" height="110" /> </div> <div class="row"> <h3>Sanger Sequencing<br>Difficult Templates</h3> <br /> </div> <div class="row"> </div> </div> </a> </li>  <li class='col-sm-3 col-centered'> <a href='/Public/Services/Sanger-Sequencing/PCR-Purification' target=''> <div class='SingleIcon headline-center Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10'> <div class="row"> <img src="/-/media/Images/Home/icons/Purple-Icons/unpurified-pcr-110x210_Purple.ashx?h=110&la=en&w=210&hash=9247F62F67970294E8877067DC4A9F66DE53D9A4" alt="Building" width="210" height="110" /> </div> <div class="row"> <h3>Sanger Sequencing<br>Un-Purified PCR Products</h3> </div> <div class="row"> </div> </div> </a> </li>  </ul> <div class="row headline-center"> </div> </div> </div>  <div class="process-v1 How-To-Order Component-BackGround-Gray margin-top-0 padding-top-10 padding-bottom-10 "> <div class=" container"> <div class="headline-center"> <div class="row"> <style> .process-v1 .process-border-4:before { border-top: none; 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Note that direct-sequencing templates are not available for our Same Day service. </div> </div> <ul class="list-inline row process-in process-border-4 col-xs-offset-0"> <li class="col-sm-3"> <div class="process-icon"> <i class="rounded-x dot-active"></i> </div> <a href='https://clims4.genewiz.com/RegisterAccount/Index' target='_blank'> <div class="process-desc"> <img src="/-/media/Images/Home/icons/Purple-Icons/create-an-account_90x90_Purple.ashx?h=90&la=en&w=90&hash=F34C22D8695A8062D12E32BD418E12867D50B0B0" alt="Building" width="90" height="90" /> </div> <div class="row"> <h3>Create Account</h3> </div> </a> </li><li class="col-sm-3"> <div class="process-icon"> <i class="rounded-x dot-active"></i> </div> <a href='https://clims4.genewiz.com' target='_blank'> <div class="process-desc"> <img src="/-/media/Images/Home/icons/Purple-Icons/place-an-order_90x90_Purple.ashx?h=90&la=en&w=90&hash=988021540FFC23F0D2CB56B780C2CBDEFC0ADEF0" alt="Building" width="90" height="90" /> </div> <div 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