Sterilization | Infection Control

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aria-label="Main Content Area"> <section class="cdc-page-title-bar cdc-page-offset noindex"> <time class="cdc-page-title-bar__item cdc-page-title-bar__item--date" datetime="2023-11-28 05:00:00"> November 28, 2023 </time> </section> <div class="cdc-page-title cdc-page-offset syndicate "> <ul class="breadcrumbs"> </ul> <h1>Sterilization</h1> <div class="page-subtitle">Guideline for Disinfection and Sterilization in Healthcare Facilities (2008)</div></div> <div id="content" class="cdc-page-content cdc-page-offset syndicate container"> <div class="cdc-dfe-body "> <div class="cdc-dfe-body__top "> <div class="dfe-section dfe-section--page-summary summary-blurb summary-paragraph" data-section="cdc_generic_summary"> <div class="dfe-section__header"> <h2>What to know</h2> </div> <div class="dfe-section__content"> <p>Sterilization from the Guideline for Disinfection and Sterilization in Healthcare Facilities (2008).</p> <div class="dfe-section__nav hide" id="dfe-section__nav"> <div class="dfe-section__nav__header" id="dfe-section__nav__header"> </div> <div class="dfe-section__nav-links" id="dfe-section__nav-links"> </div> </div> </div> <div id="nav-buttons"> </div> </div> </div> <div class="cdc-dfe-body__center "> <div class="dfe-section" data-section="cdc_generic_section_1"> <a class="onThisPageAnchor" id="cdc_generic_section_1-sterilization" name="Sterilization" title="Sterilization"></a><h2>Sterilization</h2> <p>Most medical and surgical devices used in healthcare facilities are made of materials that are heat stable and therefore undergo heat, primarily steam, sterilization. However, since 1950, there has been an increase in medical devices and instruments made of materials (e.g., plastics) that require low-temperature sterilization. Ethylene oxide gas has been used since the 1950s for heat- and moisture-sensitive medical devices. Within the past 15 years, a number of new, low-temperature sterilization systems (e.g., hydrogen peroxide gas plasma, peracetic acid immersion, ozone) have been developed and are being used to sterilize medical devices. This section reviews sterilization technologies used in healthcare and makes recommendations for their optimum performance in the processing of medical devices.<sup>1, 18, 811-820</sup></p> <p>Sterilization destroys all microorganisms on the surface of an article or in a fluid to prevent disease transmission associated with the use of that item. While the use of inadequately sterilized critical items represents a high risk of transmitting pathogens, documented transmission of pathogens associated with an inadequately sterilized critical item is exceedingly rare.<sup>821, 822</sup> This is likely due to the wide margin of safety associated with the sterilization processes used in healthcare facilities. The concept of what constitutes "sterile" is measured as a probability of sterility for each item to be sterilized. This probability is commonly referred to as the sterility assurance level (SAL) of the product and is defined as the probability of a single viable microorganism occurring on a product after sterilization. SAL is normally expressed a 10<sup>−n</sup>. For example, if the probability of a spore surviving were one in one million, the SAL would be 10<sup>−6</sup>.<sup>823, 824</sup> In short, a SAL is an estimate of lethality of the entire sterilization process and is a conservative calculation. Dual SALs (e.g., 10<sup>−3</sup>SAL for blood culture tubes, drainage bags; 10<sup>−6</sup> SAL for scalpels, implants) have been used in the United States for many years and the choice of a 10<sup>−6</sup>SAL was strictly arbitrary and not associated with any adverse outcomes (e.g., patient infections).<sup>823</sup></p> <p>Medical devices that have contact with sterile body tissues or fluids are considered critical items. These items should be sterile when used because any microbial contamination could result in disease transmission. Such items include surgical instruments, biopsy forceps, and implanted medical devices. If these items are heat resistant, the recommended sterilization process is steam sterilization, because it has the largest margin of safety due to its reliability, consistency, and lethality. However, reprocessing heat- and moisture-sensitive items requires use of a low-temperature sterilization technology (e.g., ethylene oxide, hydrogen peroxide gas plasma, peracetic acid).<sup>825</sup> A summary of the advantages and disadvantages for commonly used sterilization technologies is presented in Table 6.</p> </div> <div class="dfe-section" data-section="cdc_generic_section_2"> <a class="onThisPageAnchor" id="cdc_generic_section_2-sections" name="Sections" title="Sections"></a><h2>Sections</h2> <ul class="nested-list"> <li class="level-1"><a href="/infection-control/hcp/disinfection-sterilization/steam-sterilization.html" class="ce-selection"><strong>Steam Sterilization</strong></a><ul> </ul> </li> <li class="level-1"><a href="/infection-control/hcp/disinfection-sterilization/flash-sterilization.html"><strong>Flash Sterilization</strong></a><ul> </ul> </li> <li class="level-1"><a href="/infection-control/hcp/disinfection-sterilization/low-temperature-sterilization.html"><strong>Low-Temperature Sterilization Technologies</strong></a><ul> </ul> </li> <li class="level-1"><a href="/infection-control/hcp/disinfection-sterilization/ethylene-oxide-sterilization.html" class=""><strong>Ethylene Oxide "Gas" Sterilization</strong></a><ul> </ul> </li> <li class="level-1"><a href="/infection-control/hcp/disinfection-sterilization/hydrogen-peroxide-gas-plasma-file-name.html" class=""><strong>Hydrogen Peroxide Gas Plasma</strong></a><ul> </ul> </li> <li class="level-1"><a href="/infection-control/hcp/disinfection-sterilization/peracetic-acid-sterilization.html" class=""><strong>Peracetic Acid Sterilization</strong></a><ul> </ul> </li> <li class="level-1"><a href="/infection-control/hcp/disinfection-sterilization/microbicidal-activity-low-temperature-sterilization.html" class=""><strong>Microbicidal Activity of Low-Temperature Sterilization Technologies</strong></a><ul> </ul> </li> <li class="level-1"><a href="/infection-control/hcp/disinfection-sterilization/bioburden-surgical-devices.html" class=""><strong>Bioburden of Surgical Devices</strong></a><ul> </ul> </li> <li class="level-1"><a href="/infection-control/hcp/disinfection-sterilization/cleaning-effect-sterilization.html" class=""><strong>Effect of Cleaning on Sterilization Efficacy</strong></a><ul> </ul> </li> <li class="level-1"><a href="/infection-control/hcp/disinfection-sterilization/other-sterilization-methods.html" class=""><strong>Other Sterilization Methods</strong></a><ul> </ul> </li> <li class="level-1"><a href="/infection-control/hcp/disinfection-sterilization/sterilizing-practices.html" class=""><strong>Sterilizing Practices</strong></a><ul> </ul> </li> </ul> </div> </div> <div class="cdc-dfe-body__right page-right-rail"> <aside class="page-right-rail__dynamic noindex" role="complementary"> <div data-related-nav></div> </aside> </div> </div> <div data-journey-next></div> <section 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