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data-title="Why Single Cell" class="n2-ss-slide n2-ow n2-ss-slide-20"><div role="note" class="n2-ss-slide--focus" tabindex="-1">Why Single Cell</div><div class="n2-ss-layers-container n2-ss-slide-limiter n2-ow"><div class="n2-ss-layer n2-ow n-uc-01mHZvU2XPkm" data-sstype="slide" data-pm="default"><div class="n2-ss-layer n2-ow n-uc-CuRg5tIoc5I0" data-pm="default" data-sstype="content" data-hasbackground="0"><div class="n2-ss-section-main-content n2-ss-layer-with-background n2-ss-layer-content n2-ow n-uc-CuRg5tIoc5I0-inner"><div class="n2-ss-layer n2-ow n2-ss-layer--block n2-ss-has-self-align n-uc-mAjRB6sjy3gY" data-pm="normal" data-sstype="row"><div class="n2-ss-layer-row n2-ss-layer-with-background n-uc-mAjRB6sjy3gY-inner"><div class="n2-ss-layer-row-inner "><div class="n2-ss-layer n2-ow n-uc-162773a5550f9" data-pm="default" data-sstype="col"><div class="n2-ss-layer-col n2-ss-layer-with-background n2-ss-layer-content n-uc-162773a5550f9-inner"></div></div><div class="n2-ss-layer n2-ow n-uc-Pp4uMijtzu9n" data-pm="default" data-sstype="col"><div class="n2-ss-layer-col n2-ss-layer-with-background n2-ss-layer-content n-uc-Pp4uMijtzu9n-inner"><div class="n2-ss-layer n2-ow n-uc-oxFww95Mu0QC" data-pm="normal" data-sstype="layer"><h1 id="n2-ss-9item1" class="n2-font-70fd489f7531b6875668867099f53e68-hover n2-ss-item-content n2-ss-text n2-ow" style="display:block;">Join the Resolution Revolution</h1></div><div class="n2-ss-layer n2-ow n-uc-GiHvYnd4Dasx" data-pm="normal" data-sstype="layer"><div class="n2-ss-item-content n2-ss-text n2-ow-all"><div class=""><p class="n2-font-c9428e7d51b0ff6aa8a3a5d9bc3fefba-paragraph ">Single cell ushered in a radical level of detail. Researchers using Mission Bio can move beyond bulk methods and assess samples at the cellular level to develop critical therapies.</p> </div></div></div><div class="n2-ss-layer n2-ow n2-ss-layer--block n2-ss-has-self-align n-uc-1769510c6e89d" data-pm="normal" data-sstype="row"><div class="n2-ss-layer-row n2-ss-layer-with-background n-uc-1769510c6e89d-inner"><div class="n2-ss-layer-row-inner "><div class="n2-ss-layer n2-ow n-uc-198fc7f590a3a" data-pm="default" data-sstype="col"><div class="n2-ss-layer-col n2-ss-layer-with-background n2-ss-layer-content n-uc-198fc7f590a3a-inner"><div class="n2-ss-layer n2-ow n-uc-12e37e780bdfe n2-ss-layer--auto" data-pm="normal" data-sstype="layer"><div class="n2-ss-button-container n2-ss-item-content n2-ow n2-font-46246db450cab399df1d2efca599e90c-link n2-ss-nowrap n2-ss-button-container--non-full-width"><a class="n2-style-2667a539f0de8ce62f4c63b489d5bffe-heading n2-ow v-lightbox" href="https://fast.wistia.net/embed/iframe/ibprppwa0s?autoplay=true"><div><div>View Video</div></div></a></div></div></div></div><div class="n2-ss-layer n2-ow n-uc-141e0ca9c85ff" data-pm="default" data-hidetabletportrait="1" data-hidemobileportrait="1" data-sstype="col"><div class="n2-ss-layer-col n2-ss-layer-with-background n2-ss-layer-content n-uc-141e0ca9c85ff-inner"></div></div><div class="n2-ss-layer n2-ow n-uc-1798d0ded328b" data-pm="default" data-hidetabletportrait="1" data-hidemobileportrait="1" data-sstype="col"><div class="n2-ss-layer-col n2-ss-layer-with-background n2-ss-layer-content n-uc-1798d0ded328b-inner"></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div> </div> </div> </div> </div> </div><ss3-loader></ss3-loader></div></div><div class="n2_clear"></div></div></ss3-force-full-width></div> <div class="panels-categories wf-section"> <div class="_1300-container inner-content capabilities-links"> <div class="panels-links"> <a href="#Single-cell" class="cap-link">WHY SINGLE CELL</a> <a href="#Multi-omics" class="cap-link">WHY MULTI-OMICS</a> <a href="#Applications" class="cap-link">APPLICATIONS</a> </div> </div> </div> <div id="Single-cell" class="panels-section wf-section"> <div class="_1300-container inner-content key-benefits contains-img-caption"> <h2 id="Key" class="h2 c e">Why Single Cell?</h2> <p class="mb30-copy _60">Over the past decade, numerous advances in biotechnology have translated to improvements in the medical arena. Yet, limitations in analytical tools still remain and present significant roadblocks to the study of pathologies and the development of life-changing therapies.</p> <p class="mb30-copy _60">Today, many genotyping workflows rely on bulk assays, which mix DNA across cells and report average readouts. Although appropriate for some questions, these assays do not preserve the information of individual cells, and therefore provide only limited knowledge about biological complexity. Single-cell DNA sequencing, on the other hand, evaluates the genotype of individual cells, and thus provides a richer picture of the sample.</p> <p class="mb30-copy _60">This is especially pertinent for applications where information at the cellular level matters. Some of the most pressing challenges include elucidating the complexity of heterogeneous diseases like cancer, and the underlying mechanisms driving them. Other hurdles lie within the process of pharmacological development, in which each therapeutic candidate must be characterized with sufficient granularity to ensure safety and efficacy.</p> <div class="wp-caption aligncenter"> <img loading="lazy" class="size-full" src="/wp-content/themes/missionbio-v2/images-2/App_Why_Single_Cell_Infographic_Img01.png" width="1000" alt="Bulk Sequencing vs Single-cell DNA Sequencing"> <p class="wp-caption-text no-heading">In conventional bulk sequencing (left), DNA is mixed across cells such that genetically distinct subpopulations (e.g. subclones) cannot be identified. Genotypic measurements (e.g. variant allele frequencies, VAF) therefore represent a population average. In single-cell DNA sequencing (right), genetic targets are barcoded in each cell. This allows for genotypic readouts for different subpopulations of cells.</p> </div> </div> </div> <div id="Multi-omics" class="why-multi wf-section"> <div class="_1300-container inner-content contains-img-caption"> <h2 id="Key" class="h2 c">Why Multi-omics?</h2> <p class="mb30-copy _60">Conventional workflows in disease research and therapeutic development involve running multiple separate assays, often on different instruments, to get answers. This approach has several drawbacks.</p> <p class="mb30-copy _60">First, precious samples that are difficult to obtain must be used in multiple assays — leaving little for downstream analyses. Second, the data integration and analysis of multi-assay studies is complex and typically requires bioinformatics expertise to glean correlations across different datasets. Third, conducting multiple assays on different “omics” platforms can get expensive, and will often require longer timelines before researchers get answers to their experimental questions.</p> <p class="mb30-copy _60">Fortunately, innovative multi-omics technologies like the <a href="https://missionbio.com/products/platform/">Tapestri Platform</a> are offering a solution to these issues. Tapestri simultaneously measures DNA and cell-surface proteins across thousands of individual cells.</p> <p class="mb30-copy _60">By collapsing genotypic and phenotypic assays into a single workflow, Tapestri both preserves precious samples and enables multi-parameter data to be gathered quickly and easily. This powerful approach is offering an unprecedented look into the nuances of biology and transforming how we explore disease and develop therapies.</p> <div class="wp-caption aligncenter"> <img loading="lazy" class="size-full" src="/wp-content/themes/missionbio-v2/images-2/App_Why_Single_Cell_Infographic_Img02b.png" alt="Illustration of ex vivo and in vivo cell and gene therapies."> <p class="wp-caption-text no-heading white-bg">Different technologies enable varying levels of resolution in the fields of oncology and cell and gene therapies. Whereas bulk sequencing portrays only population-level metrics of cells, single-cell sequencing and multi-omics oncover cell-to-cell variation in a variety of aspects relevant to cancer and advanced therapeutics.</p> </div> </div> </div> <div id="Applications" class="panels-section margins wf-section pt0"> <div class="_1300-container inner-content key-benefits"> <h2 class="h2-other">Single-cell Applications</h2> <p class="mb30-copy _60">Single-cell analysis is advancing several scientific areas, including oncology and the development of advanced therapeutics. For instance, Tapestri is helping advance cancer translational research, where information about tumor architecture can be used to inform diagnosis, prognosis, and treatment strategies in a highly personalized fashion.</p> <p class="mb30-copy _60">Tapestri is also helping advance the development of novel cell and gene therapies. By measuring multiple genotypic alterations across cells, therapy developers achieve deeper characterization of gene-modified cell therapies and assess in vivo gene therapies in model systems.</p> <p class="mb30-copy">The benefits of single-cell analysis extend to academia and industries alike. Pharma and biopharma can capitalize on the highly sensitive analysis of Tapestri to assess therapy resistance, MRD, and characterize cell-based drug candidates.</p> </div> <div class="applications-cols _1300"> <a href="https://missionbio.com/applications/oncology/" class="h-benefits-cols onco-3 w-inline-block" data-ix="applications-hover"> <div class="h-application-overlay-disabled"></div> <div class="h-application-content"> <div class="h-application-title">Oncology</div> <div>Deeply characterize the genotype and phenotype of advanced therapeutics</div> <div class="button-2 w mt"> <div>LEARN MORE</div> </div> </div> </a> <a href="https://missionbio.com/applications/cell-and-gene-therapy/" class="h-benefits-cols cell w-inline-block" data-ix="applications-hover"> <div class="h-application-overlay-disabled"></div> <div class="h-application-content"> <div class="h-application-title">Cell and Gene Therapy</div> <div>Develop safe and efficacious solutions with single-cell analysis</div> <div class="button-2 w mt"> <div>LEARN MORE</div> </div> </div> </a> </div> </div> <div id="Resources" class="h-resources"> <div class="_1300-container inner-content"> <h3 class="h3 w">Featured Resources</h3> <div class="h-resources-grid"> <div class="h-resource-card eb" data-ix="show-more-link"> <div class="h-resource-image eb" ></div> <div class="h-resource-content"> <div class="h-resource-category d eb">ebook</div> <a href="https://missionbio.com/resources/ebooks/single-cell-sequencing-101/" class="h-resource-title-link"> Single-cell Sequencing 101: DNA & multi-omics for precision medicine </a> <br /> <a href="https://missionbio.com/resources/ebooks/single-cell-sequencing-101/" class="more-link">LEARN MORE</a> </div> </div> <div class="h-resource-divider"></div> <div class="h-resource-card b" data-ix="show-more-link"> <div class="h-resource-image b" style="background-image: url(/wp-content/themes/missionbio-v2/images-2/brochures.jpg);"></div> <div class="h-resource-content"> <div class="h-resource-category d br">brochure</div> <a href="https://missionbio.com/resources/brochures/platform-brochure/" class="h-resource-title-link"> Tapestri Platform </a> <br /> <a href="https://missionbio.com/resources/brochures/platform-brochure/" class="more-link">LEARN MORE</a> </div> </div> <div class="h-resource-divider"></div> <div class="h-resource-card tn" data-ix="show-more-link"> <div class="h-resource-image tn" ></div> <div class="h-resource-content"> <div class="h-resource-category d tn">technical note</div> <a href="https://missionbio.com/resources/technical-notes/correlations-between-genomics-variants-and-protein-expression/" class="h-resource-title-link"> Single-Cell Multi-Omics Reveals Novel Correlations Between Genomic Variants and Protein Expression in AML Patient Samples </a> <br /> <a href="https://missionbio.com/resources/technical-notes/correlations-between-genomics-variants-and-protein-expression/" class="more-link">LEARN MORE</a> </div> </div> <div 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