DNA Libraries | GENEWIZ from Azenta

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Walking</a></li><br><li><a class='GColor' href='/en/Public/Services/Molecular-Genetics' style='color:#826ec3;font-size:17px'>PCR + Sanger Services</a></li><li><a href='/en/Public/Services/Molecular-Genetics/Bacterial-and-Fungal-Identification' style='color:#585F69;font-size:14px'>Bacterial and Fungal Identification</a></li><li><a href='/en/Public/Services/Molecular-Genetics/Mutation-Analysis' style='color:#585F69;font-size:14px'>Mutation Analysis</a></li><li><a href='/en/Public/Services/Molecular-Genetics/SNP-Genotyping' style='color:#585F69;font-size:14px'>SNP Genotyping</a></li><li><a href='/en/Public/Services/Molecular-Genetics/PCR-Plus-Sequencing-CRISPR-Analysis-Package' style='color:#585F69;font-size:14px'>PCR Plus Sequencing CRISPR Analysis Package</a></li><li><a href='/en/Public/Services/Molecular-Genetics/cDNA-Verification' style='color:#585F69;font-size:14px'>Confirmatory Sequencing & Cell Bank Characterization</a></li><li><a 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href='/en/Public/Services/Gene-Synthesis/Antibody-DNA-Synthesis' style='color:#585F69;font-size:14px'>Antibody Synthesis</a></li><li><a href='/en/Public/Services/Gene-Synthesis/AAV-Plasmid-Synthesis' style='color:#585F69;font-size:14px'>AAV Plasmid Synthesis</a></li><li><a href='/en/Public/Services/Gene-Synthesis/Gene-Fragments' style='color:#585F69;font-size:14px'>Gene Fragments</a></li><li><a href='/en/Public/Services/Gene-Synthesis/CRISPR-Construct-Synthesis' style='color:#585F69;font-size:14px'>CRISPR Construct Synthesis</a></li><li><a href='/en/Public/Services/Gene-Synthesis/Synthetic-DNA-Libraries' style='color:#585F69;font-size:14px'>Synthetic DNA Libraries</a></li><li><a href='/en/Public/Services/Gene-Synthesis/Single-Stranded-DNA-Synthesis' style='color:#585F69;font-size:14px'>ssDNA Synthesis</a></li><li><a href='/en/Public/Services/Gene-Synthesis/DNA-Cloning' style='color:#585F69;font-size:14px'>DNA Cloning</a></li><li><a href='/en/Public/Services/Gene-Synthesis/Site-Directed-Mutagenesis' style='color:#585F69;font-size:14px'>Site-Directed Mutagenesis<br></a></li><li><a href='/en/Public/Services/Gene-Synthesis/Viral-Package' style='color:#585F69;font-size:14px'>Viral Packaging <sup style="color:#826ec3;"><strong>NEW </strong></sup></a></li><li><a href='/en/Public/Services/Gene-Synthesis/Recombinant-Antibody-Production' style='color:#585F69;font-size:14px'>Recombinant Antibody Production <sup style="color:#826ec3;"><strong>NEW </strong></sup></a></li><li><a href='/en/Public/Services/Gene-Synthesis/Custom-mRNA-Synthesis' style='color:#585F69;font-size:14px'>Custom mRNA Synthesis <sup style="color:#826ec3; font-weight: bold;">NEW</sup></a></li><br><li><a class='GColor' href='/en/Public/Services/Oligo' style='color:#826ec3;font-size:17px'>Oligo Synthesis Services</a></li><li><a href='/en/Public/Services/Oligo/Overnight-DNA-Oligos' style='color:#585F69;font-size:14px'>Overnight DNA Oligos</a></li><li><a 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Whole Genome Sequencing</a></li><li><a href='/en/Public/Services/Clinical-Services/Regulatory' style='color:#585F69;font-size:14px'>GLP-Compliant Overview</a></li><li><a href='/en/Public/Services/Clinical-Services/GLP-compliant-Confirmatory-Sequencing' style='color:#585F69;font-size:14px'>GLP Confirmatory Sequencing</a></li><li><a href='/en/Public/Services/Clinical-Services/GLP-compliant-SNP-Mutation-Analysis' style='color:#585F69;font-size:14px'>GLP SNP Mutation Analysis</a></li><li><a href='/en/Public/Services/Clinical-Services/GLP-compliant-Plasmid-Prep' style='color:#585F69;font-size:14px'>GLP Plasmid Prep</a></li><li><a href='/en/Public/Services/Clinical-Services/Nucleic-Acid-Extraction' style='color:#585F69;font-size:14px'>GLP Nucleic Acid Extraction</a></li><li><a href='/en/Public/Services/Clinical-Services/Biofluid-Processing-Overview' style='color:#585F69;font-size:14px'>Biofluid Processing</a></li><li><a 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} .column1 { float: left; width: 50%; padding: 10px; } .column2 { float: left; width: 50%; padding: 10px; } .row:after { content: ""; display: table; clear: both; } .Libraries-content-box { display: inline-flex; vertical-align: left; } #Libraries-content-box-left { width: 60%; text-align: left; padding: 30px 0; } #Libraries-content-box-right { width: 39%; text-align: left; } .Libraries-content-box img { width: 450px; height: 110px; float: left; } .Libraries-content-box button { border: none; border-radius: 4px; background: #826ec3; padding: 10px 20px; color: #fff; font-size: 10px; letter-spacing: 1px; } .Libraries-content-box button:hover { background: #6965a1; } @media only screen and (max-width: 1170px) { .Libraries-content-box { max-width: 100%; } .Libraries-content-box img { max-width: 450px; max-height: 110px; float: left; } } @media only screen and (max-width: 768px) { .Libraries-content-box { display: block; width: 100%; } </style> <div class="container margin-bottom-30 margin-top-50" style="text-align:left;"> <div class="Libraries-new-content"> <h2 style="text-align: center;">DNA Library Services</h2> <div class="Libraries-content-box"> <div class="row"> <div class="column1" style="background-color:#F0F0F0;"> <h3 style="text-align: center;">Combinatorial (Classical)</h3> <p style="text-align: center;">Create a library of completely randomized (NNN) or partially randomized (NNK/NNS) mutants with multiple, different combinations of genetic elements.</p> </div> <div class="Libraries-content-box" id="Libraries-content-box-right"> <img alt="" src="/-/media/Images/12007-D_SyntheticDNALib_Combinatorial-Img_new.ashx?la=en&hash=73B8D72DCF53332912FC373B86ADC9B80B989190" /> </div> <div class="container margin-bottom-30 margin-top-50" style="text-align:left;"> <div class="Libraries-new-content"> <div class="Libraries-content-box"> <div class="row"> <div class="column1" style="background-color:#F0F0F0;"> <h3 style="text-align: center;">Trimer-Controlled (Smart)</h3> <p style="text-align: center;">Specify an exact combination of codons, a precise percentage of desired amino acids (as low as 5%) and reduce frameshift mutations for the best mutant integrity.</p> </div> <div class="Libraries-content-box" id="Libraries-content-box-right"> <img alt="" src="/-/media/Images/12007-D_SyntheticDNALib_TrimerControlled-Img_new.ashx?la=en&hash=9D25BEBC8B5F43B33FD4BFEC4B6D48A4E5E7973C" /> </div> <div class="container margin-bottom-30 margin-top-50" style="text-align:left;"> <div class="Libraries-new-content"> <div class="Libraries-content-box"> <div class="row"> <div class="column1" style="background-color:#F0F0F0;"> <h3 style="text-align: center;">Variant</h3> <p style="text-align: center;">Introduce singular/scanning site-directed mutagenesis in a user-defined 24 base pair region or truncate a sequence to characterize and optimize the desired protein features and function.</p> </div> <div class="Libraries-content-box" id="Libraries-content-box-right"> <img alt="" src="/-/media/Images/12007-D_SyntheticDNALib_Variant-Img_new.ashx?la=en&hash=A5734389285254CE02BF8E36FE2C1170C453832C" /> </div> <div class="container margin-bottom-30 margin-top-50" style="text-align:left;"> <div class="Libraries-new-content"> <div class="Libraries-content-box"> <div class="row"> <div class="column1" style="background-color:#F0F0F0;"> <h3 style="text-align: center;">sgRNA</h3> <p style="text-align: center;">Accelerate CRISPR screening programs with single guide RNA (sgRNA) libraries containing knock-out/knock-in variants. Choose from pre-made or custom generated offerings.</p> </div> <div class="Libraries-content-box" id="Libraries-content-box-right"> <img alt="" src="/-/media/Images/12007-D_SyntheticDNALib_sgRNA-Img_new.ashx?la=en&hash=F754A6A2C46BF77A10418A63DC1E10C95507D470" /> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> .body { text-align: center; display: inline-block; } .banner3 { width: 1170px; background-image: url('/-/media/12054-D_Gene-DIscovery-Solutions_WebBanFull-Med_BG.ashx'); background-size: cover; text-align: left; display: flex; padding: 10px; } .banner3 img { text-align: center; display: block; margin-left: auto; margin-right: auto; } #banner3-left { width: 50%; padding: 20px; } #banner3-right { width: 49%; padding: 10px; } #banner3-right h2 { text-transform: none; letter-spacing: 1px; line-height: 50px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 35px; margin: 0; margin-bottom: 5px; color: #FFFFFF } #banner3-left h2 { text-transform: none; letter-spacing: 1px; line-height: 50px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 35px; margin: 5; margin-bottom: 0px; color: #FFFFFF } #banner3-right h3 { text-transform: none; letter-spacing: 1px; line-height: 29px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 20px; margin: 0; margin-bottom: 5px; color: #FFFFFF } #banner3-left h3 { text-transform: none; letter-spacing: 1px; line-height: 29px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 20px; margin: 0; margin-bottom: 5px; color: #FFFFFF } #banner3-right button { padding: 8px 19px; color: #826ec3; letter-spacing: 1px; background: #FFFFFF; border: 2px solid #826ec3; border-radius: 3px; transition: all .2s ease-in-out; font-size: 18px; font-weight: bold; } #banner3-left button { padding: 7px 19px; color: #826ec3; letter-spacing: 1px; background: #FFFFFF; border: 2px solid #826ec3; border-radius: 3px; transition: all .2s ease-in-out; font-size: 17px; font-weight: bold; } #banner3-right button:hover { cursor: pointer; transform: scale(1.1); } #banner3-left button:hover { cursor: pointer; transform: scale(1.1); } #banner3-right button:focus { outline: 0; } #banner3-left button:focus { outline: 0; } @media only screen and (max-width: 1170px) { .banner3 { width: 95%; height: auto; background-image: url('/-/media/12054-D_Gene-DIscovery-Solutions_WebBanFull-Med_BG.ashx'); display: inline-block; } #banner3-left { text-align: center; width: 100%; } #banner3-right { text-align: center; width: 100%; } #banner3-right h2 { text-transform: none; letter-spacing: 1px; line-height: 50px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 40px; margin: 0; margin-bottom: 15px; color: #FFFFFF } #banner3-right h3 { text-transform: none; letter-spacing: 1px; line-height: 50px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 30px; margin: 0; margin-bottom: 15px; color: #FFFFFF } #banner3-left img { width: auto; height: auto; } #banner3-right img { width: 90%; height: auto; } @media only screen and (max-width: 700px) { #banner3-left img { width: 60%; } #banner3-right button { font-size: 14px; } } </style> <div class="body"> <div class="banner3"> <div id="banner3-left"> <h3 style="text-align:left;">STREAMLINE YOUR PATH TO DISCOVERY</h3> <h2 style="text-align:left;">Gene-to-Discovery Solutions</h2> <h3 style="text-align:left;">AAV Packaging, Custom mRNA Synthesis, Lentivirus Packaging, Recombinant Antibody Production</h3> <br /> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://hubs.ly/Q02gBwzs0" target="_blank"><button style="text-align:left;">LEARN MORE</button></a> </div> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <div class="container margin-bottom-30 margin-top-50" style="text-align:left;"> <h2 style="text-align:center;">Featured Application: Antibody Development</h2> <p>The success of an <em>in-vitro</em> antibody discovery and development program is contingent on the diversity of functional candidates as well as the ability to enhance favorable characteristics. Choose Azenta’s combinatorial or trimer-controlled libraries to expedite your antibody discovery and development program while keeping the cost low.</p> <ul> <li><strong>Combinatorial DNA libraries</strong> A powerful tool for determining and optimizing biomolecule function from a parent sequence. By coupling these libraries with a screening technology such as phage display, it becomes possible for researchers to both develop and test an immense number of mutant variants in a short time. Depending on the research goals, budget, and timeline, different mutagenesis techniques can be used to create variants. The below table compares three industry standard approaches. </li> </ul> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <div class="container text-center"> <div class="panel-heading"> <h3 class="panel-title text-center"></h3> </div> <table class="table table-bordered"> <tbody> <tr> <td style="background-color: #826ec3; color:#FFFFFF;"><strong>Library</strong></td> <td style="background-color: #826ec3; color:#FFFFFF;"><strong>Stop Codons</strong></td> <td style="background-color: #826ec3; color:#FFFFFF;"><strong>Risk of Mutation</strong></td> <td style="background-color: #826ec3; color:#FFFFFF;"><strong>'AT' Rich Bias </strong></td> <td style="background-color: #826ec3; color:#FFFFFF;"><strong>Assigned Amino Acids </strong></td> <td style="background-color: #826ec3; color:#FFFFFF;"><strong> Screening Speed</strong></td> <td style="background-color: #826ec3; color:#FFFFFF;"><strong>Screening Costs</strong></td> </tr> <tr> <td> <p>Random (NNN)</p> </td> <td>3</td> <td><span>High</span></td> <td>Possible</td> <td>No</td> <td>Slow</td> <td>$$$</td> </tr> <tr> <td> <p style="margin-left: 0in;">Partially Random (NNK/NNS)</p> </td> <td>1</td> <td>High</td> <td>Possible</td> <td>No</td> <td>Moderate</td> <td>$$</td> </tr> <tr> <td> <p style="margin-left: 0in;">Trimer-Controlled</p> </td> <td>0</td> <td> <p style="margin-left: 0in;">Low</p> </td> <td> None</td> <td>Yes</td> <td>Fastest</td> <td>$</td> </tr> </tbody> </table> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <div class="container margin-bottom-30 margin-top-50" style="text-align:left;"> <ul> <li><strong>Trimer-controlled libraries:</strong> An effective tool that significantly reduces screening efforts compared to conventional mutagenesis methods, empower researchers with the most accuracy, precision, and control as compared to other combinatorial DNA library techniques because of the extra synthetic effort devoted to restricting codon building blocks. Download the case study below to learn why researchers at a large bio-pharmaceutical company chose Azenta combinatorial trimer-controlled synthesis of antibody libraries to obtain a highly accurate mutant library.</li> </ul> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> .body { text-align: center; display: inline-block; } .banner { width: 1170px; background-image: url('/-/media/Images/12007-D_Trimer-Controlled_Web-Ban-Full-Lrg-Mockup-BG_v2.ashx'); background-size: cover; text-align: center; display: flex; padding: 10px; } .banner img { text-align: center; display: block; margin-left: auto; margin-right: auto; } #banner-left { width: 40%; padding: 20px; } #banner-right { width: 80%; padding: 10px; } #banner-right h1 { text-transform: none; letter-spacing: 1px; line-height: 50px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 40px; margin: 0; margin-bottom: 5px; color: #FFFFFF } #banner-right h3 { text-transform: none; letter-spacing: 1px; line-height: 50px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 20px; margin: 0; margin-bottom: 5px; color: #FFFFFF } #banner-right button { padding: 8px 19px; color: #826ec3; letter-spacing: 1px; background: #FFFFFF; border: 2px solid #55c8d2; border-radius: 3px; transition: all .2s ease-in-out; font-size: 18px; font-weight: bold; } #banner-right button:hover { cursor: pointer; transform: scale(1.1); } #banner-right button:focus { outline: 0; } @media only screen and (max-width: 1170px) { .banner { width: 95%; height: auto; background: #55c8d2; display: inline-block; } #banner-left { text-align: center; width: 100%; } #banner-right { text-align: center; width: 100%; } #banner-right h1 { text-transform: none; letter-spacing: 1px; line-height: 50px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 45px; margin: 0; margin-bottom: 15px; color: #FFFFFF; } #banner-right h3 { text-transform: none; letter-spacing: 1px; line-height: 50px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 30px; margin: 0; margin-bottom: 15px; color: #FFFFFF; text-align: center; } #banner-left img { width: auto; height: auto; } #banner-right img { width: 90%; height: auto; } @media only screen and (max-width: 700px) { .banner { background: #55c8d2; } #banner-left img { width: 60%; } #banner-right button { font-size: 14px; } } </style> <div class="body"> <div class="banner"> <div id="banner-left"> <img alt="" src="/-/media/Images/12007-D_Trimer-Controlled_Web-Ban-Full-Lrg-Graphic_v2.ashx?la=en&hash=DAC6637A16F98EF156C9C9CF11636A32D5F7B8A1" /> </div> <div id="banner-right"> <div id="banner-right h1"> <h1>Case Study</h1> <h1><strong>Combinatorial Trimer-Controlled Synthesis of Antibody Libraries</strong></h1> <div id="banner-right h3"> <br /> <br /> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://web.genewiz.com/case-study/trimer-controlled-synthesis-antibody-libraries" target="_blank"><button>Download</button></a> </div> </div> </div> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <div class="container text-centre"> <div class="panel-heading"> <h3 class="panel-title text-centre"></h3> </div> <h2>DNA Library Deliverables</h2> <table class="table table-bordered"> <tbody> <tr> <td style="background-color: #826ec3; color:#FFFFFF;"><strong>Library Type</strong></td> <td style="background-color: #826ec3; color:#FFFFFF;" colspan="2"><strong>Classical Combinatorial Libraries (NNN/NNK/NNS) and Trimer- Controlled (Smart libraries)</strong><br /> </td> <td style="background-color: #826ec3; color:#FFFFFF;"><strong>Variant</strong></td> </tr> <tr> <td> <p><strong>Library Format</strong></p> </td> <td>Linear DNA pool</td> <td><span>Cloned Pool</span></td> <td>Individual Clones</td> </tr> <tr> <td> <p style="margin-left: 0in;"><strong>Diversity</strong></p> </td> <td> Up to 10<sup>12</sup> variants</td> <td>Up to 10<sup>9</sup> variants</td> <td>Customer defined</td> </tr> <tr> <td> <strong style="background-color: transparent;">Vector</strong></td> <td> N/A</td> <td> Customer defined</td> <td>Customer defined</td> </tr> <tr> <td><strong>Quality Control</strong></td> <td>Sanger sequencing of up to 96 individual transformants (default) <br /> <br /> Additional NGS-based diversity analysis (optional) </td> <td>Sanger sequencing of up to 96 individual transformants (default) <br /> <br /> Additional NGS-based diversity analysis (optional) </td> <td>100% sequence accuracy guaranteed.- Restriction digest of final plasmid </td> </tr> <tr> <td><strong>Deliverables</strong></td> <td>1-2 µg of linear dsDNA <br /> <br /> Certificate of Analysis (COA) with quality measurements.</td> <td>5-10 µg of plasmid DNA <br /> <br /> Certificate of Analysis (COA) with quality measurements. <br /> <br /> Large scale DNA preparation options ranging from µg to mg.</td> <td>2-5 µg of plasmid DNA (standard) <br /> <br /> Certificate of Analysis (COA) with quality measurements. <br /> <br /> Large scale DNA preparation options ranging from µg to mg.</td> </tr> </tbody> </table> </div> <p style="text-align:center;">If you have custom requests or require any pre-project consultation, please contact our expert project management team at <a href="mailto:PM@azenta.com">PM@azenta.com</a></p> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> .body { text-align: center; 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} @media only screen and (max-width: 700px) { #banner-left img { width: 60%; } #banner-right button { font-size: 14px; } } </style> <div class="body"> <div class="banner1"> <div id="banner-left"> <img alt="" src="-/media/.ashx" /> </div> <div id="banner-right"> <div id="banner-right h1"> <h1 style="color: #FFF;"><strong>Antibody Libraries</strong></h1> <h1 style="color: #FFF;"><strong>for <em>In Vitro</em> Display:</strong></h1> <h1 style="color: #FFF;"><strong> What to Consider</strong></h1> <div id="banner-right h3"> <br /> <br /> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://www.azenta.com/learning-center/blog/antibody-libraries-vitro-display-what-consider?utm_campaign=Antibody Libraries Blog&utm_source=Web banner&utm_medium=Full&__hstc=251210969.531b7d6279fdab71a9b2db01e6d04a4f.1696511138918.1705332586165.1705416243631.77&__hssc=251210969.174.1705416243631&__hsfp=2731169240" target="_blank"><button>Read Article</button></a> </div> </div> </div> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area">   </div> <script type="text/javascript" src="/assets/plugins/counter/jquery.counterup.min.js"></script> <script language="javascript" type="text/javascript"> jQuery(document).ready(function ($) { $(".row .category").find("img").addClass("img-responsive"); 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