CINXE.COM

<!DOCTYPE html> <html lang="en" xmlns:fb="http://www.facebook.com/2008/fbml" class="wf-loading"> <head prefix="og: https://ogp.me/ns# fb: https://ogp.me/ns/fb# academia: https://ogp.me/ns/fb/academia#"> <meta charset="utf-8"> <meta name=viewport content="width=device-width, initial-scale=1"> <meta rel="search" type="application/opensearchdescription+xml" href="/open_search.xml" title="Academia.edu"> <title>Hare Pradhan - Academia.edu</title>  <link href="//a.academia-assets.com/images/favicons/favicon-production.ico" rel="shortcut icon" type="image/vnd.microsoft.icon"> <link rel="apple-touch-icon" sizes="57x57" href="//a.academia-assets.com/images/favicons/apple-touch-icon-57x57.png"> <link rel="apple-touch-icon" sizes="60x60" href="//a.academia-assets.com/images/favicons/apple-touch-icon-60x60.png"> <link rel="apple-touch-icon" sizes="72x72" href="//a.academia-assets.com/images/favicons/apple-touch-icon-72x72.png"> <link rel="apple-touch-icon" sizes="76x76" href="//a.academia-assets.com/images/favicons/apple-touch-icon-76x76.png"> <link rel="apple-touch-icon" sizes="114x114" href="//a.academia-assets.com/images/favicons/apple-touch-icon-114x114.png"> <link rel="apple-touch-icon" sizes="120x120" href="//a.academia-assets.com/images/favicons/apple-touch-icon-120x120.png"> <link rel="apple-touch-icon" sizes="144x144" href="//a.academia-assets.com/images/favicons/apple-touch-icon-144x144.png"> <link rel="apple-touch-icon" sizes="152x152" href="//a.academia-assets.com/images/favicons/apple-touch-icon-152x152.png"> <link rel="apple-touch-icon" sizes="180x180" href="//a.academia-assets.com/images/favicons/apple-touch-icon-180x180.png"> <link rel="icon" type="image/png" href="//a.academia-assets.com/images/favicons/favicon-32x32.png" sizes="32x32"> <link rel="icon" type="image/png" href="//a.academia-assets.com/images/favicons/favicon-194x194.png" sizes="194x194"> <link rel="icon" type="image/png" href="//a.academia-assets.com/images/favicons/favicon-96x96.png" sizes="96x96"> <link rel="icon" type="image/png" href="//a.academia-assets.com/images/favicons/android-chrome-192x192.png" sizes="192x192"> <link rel="icon" type="image/png" href="//a.academia-assets.com/images/favicons/favicon-16x16.png" sizes="16x16"> <link rel="manifest" href="//a.academia-assets.com/images/favicons/manifest.json"> <meta name="msapplication-TileColor" content="#2b5797"> <meta name="msapplication-TileImage" content="//a.academia-assets.com/images/favicons/mstile-144x144.png"> <meta name="theme-color" content="#ffffff"> <script> window.performance && window.performance.measure && window.performance.measure("Time To First Byte", "requestStart", "responseStart"); </script> <script> (function() { if (!window.URLSearchParams || !window.history || !window.history.replaceState) { return; } var searchParams = new URLSearchParams(window.location.search); var paramsToDelete = [ 'fs', 'sm', 'swp', 'iid', 'nbs', 'rcc', // related content category 'rcpos', // related content carousel position 'rcpg', // related carousel page 'rchid', // related content hit id 'f_ri', // research interest id, for SEO tracking 'f_fri', // featured research interest, for SEO tracking (param key without value) 'f_rid', // from research interest directory for SEO tracking 'f_loswp', // from research interest pills on LOSWP sidebar for SEO tracking 'rhid', // referrring hit id ]; if (paramsToDelete.every((key) => searchParams.get(key) === null)) { return; } paramsToDelete.forEach((key) => { searchParams.delete(key); }); var cleanUrl = new URL(window.location.href); cleanUrl.search = searchParams.toString(); history.replaceState({}, document.title, cleanUrl); })(); </script> <script async src="https://www.googletagmanager.com/gtag/js?id=G-5VKX33P2DS"></script> <script> window.dataLayer = window.dataLayer || []; function gtag(){dataLayer.push(arguments);} gtag('js', new Date()); gtag('config', 'G-5VKX33P2DS', { cookie_domain: 'academia.edu', send_page_view: false, }); gtag('event', 'page_view', { 'controller': "profiles/works", 'action': "summary", 'controller_action': 'profiles/works#summary', 'logged_in': 'false', 'edge': 'unknown', // Send nil if there is no A/B test bucket, in case some records get logged // with missing data - that way we can distinguish between the two cases. // ab_test_bucket should be of the form <ab_test_name>:<bucket> 'ab_test_bucket': null, }) </script> <script type="text/javascript"> window.sendUserTiming = function(timingName) { if (!(window.performance && window.performance.measure)) return; var entries = window.performance.getEntriesByName(timingName, "measure"); if (entries.length !== 1) return; var timingValue = Math.round(entries[0].duration); gtag('event', 'timing_complete', { name: timingName, value: timingValue, event_category: 'User-centric', }); }; window.sendUserTiming("Time To First Byte"); </script> <meta name="csrf-param" content="authenticity_token" /> <meta name="csrf-token" content="TFZFPWiNcw3Ay9tHGmvEqn8q71I8jK7rbFVF1oM9id+QWT4cLgiSMGcBLJpyej44EfWPPmPQ9Hp/mcOwoUQHVA==" /> <link rel="stylesheet" media="all" href="//a.academia-assets.com/assets/wow-77f7b87cb1583fc59aa8f94756ebfe913345937eb932042b4077563bebb5fb4b.css" /><link rel="stylesheet" media="all" href="//a.academia-assets.com/assets/social/home-9e8218e1301001388038e3fc3427ed00d079a4760ff7745d1ec1b2d59103170a.css" /><link rel="stylesheet" media="all" href="//a.academia-assets.com/assets/design_system/heading-b2b823dd904da60a48fd1bfa1defd840610c2ff414d3f39ed3af46277ab8df3b.css" /><link rel="stylesheet" media="all" href="//a.academia-assets.com/assets/design_system/button-3cea6e0ad4715ed965c49bfb15dedfc632787b32ff6d8c3a474182b231146ab7.css" /><link crossorigin="" href="https://fonts.gstatic.com/" rel="preconnect" /><link href="https://fonts.googleapis.com/css2?family=DM+Sans:ital,opsz,wght@0,9..40,100..1000;1,9..40,100..1000&family=Gupter:wght@400;500;700&family=IBM+Plex+Mono:wght@300;400&family=Material+Symbols+Outlined:opsz,wght,FILL,GRAD@20,400,0,0&display=swap" rel="stylesheet" /><link rel="stylesheet" media="all" href="//a.academia-assets.com/assets/design_system/common-10fa40af19d25203774df2d4a03b9b5771b45109c2304968038e88a81d1215c5.css" /> <meta name="author" content="hare pradhan" /> <meta name="description" content="Hare Pradhan: 16 Followers, 9 Following, 20 Research papers." /> <meta name="google-site-verification" content="bKJMBZA7E43xhDOopFZkssMMkBRjvYERV-NaN4R6mrs" /> <script> var $controller_name = 'works'; var $action_name = "summary"; var $rails_env = 'production'; var $app_rev = '08c611e06efd956c8cb6c100cf9e757ee1b93f16'; var $domain = 'academia.edu'; var $app_host = "academia.edu"; var $asset_host = "academia-assets.com"; var $start_time = new Date().getTime(); var $recaptcha_key = "6LdxlRMTAAAAADnu_zyLhLg0YF9uACwz78shpjJB"; var $recaptcha_invisible_key = "6Lf3KHUUAAAAACggoMpmGJdQDtiyrjVlvGJ6BbAj"; var $disableClientRecordHit = false; </script> <script> window.Aedu = { hit_data: null }; window.Aedu.SiteStats = {"premium_universities_count":15265,"monthly_visitors":"113 million","monthly_visitor_count":113485689,"monthly_visitor_count_in_millions":113,"user_count":277892299,"paper_count":55203019,"paper_count_in_millions":55,"page_count":432000000,"page_count_in_millions":432,"pdf_count":16500000,"pdf_count_in_millions":16}; window.Aedu.serverRenderTime = new Date(1733197821000); window.Aedu.timeDifference = new Date().getTime() - 1733197821000; window.Aedu.isUsingCssV1 = false; window.Aedu.enableLocalization = true; window.Aedu.activateFullstory = false; window.Aedu.serviceAvailability = { status: {"attention_db":"on","bibliography_db":"on","contacts_db":"on","email_db":"on","indexability_db":"on","mentions_db":"on","news_db":"on","notifications_db":"on","offsite_mentions_db":"on","redshift":"on","redshift_exports_db":"on","related_works_db":"on","ring_db":"on","user_tests_db":"on"}, serviceEnabled: function(service) { return this.status[service] === "on"; }, readEnabled: function(service) { return this.serviceEnabled(service) || this.status[service] === "read_only"; }, }; window.Aedu.viewApmTrace = function() { // Check if x-apm-trace-id meta tag is set, and open the trace in APM // in a new window if it is. var apmTraceId = document.head.querySelector('meta[name="x-apm-trace-id"]'); if (apmTraceId) { var traceId = apmTraceId.content; // Use trace ID to construct URL, an example URL looks like: // https://app.datadoghq.com/apm/traces?query=trace_id%31298410148923562634 var apmUrl = 'https://app.datadoghq.com/apm/traces?query=trace_id%3A' + traceId; window.open(apmUrl, '_blank'); } }; </script>  <link href="https://fonts.googleapis.com/css?family=Roboto:100,100i,300,300i,400,400i,500,500i,700,700i,900,900i" rel="stylesheet"> <link href="//maxcdn.bootstrapcdn.com/font-awesome/4.3.0/css/font-awesome.min.css" rel="stylesheet"> <link rel="stylesheet" media="all" href="//a.academia-assets.com/assets/libraries-a9675dcb01ec4ef6aa807ba772c7a5a00c1820d3ff661c1038a20f80d06bb4e4.css" /> <link rel="stylesheet" media="all" href="//a.academia-assets.com/assets/academia-bdb9e8c097f01e611f2fc5e2f1a9dc599beede975e2ae5629983543a1726e947.css" /> <link rel="stylesheet" media="all" href="//a.academia-assets.com/assets/design_system_legacy-056a9113b9a0f5343d013b29ee1929d5a18be35fdcdceb616600b4db8bd20054.css" /> <script src="//a.academia-assets.com/assets/webpack_bundles/runtime-bundle-005434038af4252ca37c527588411a3d6a0eabb5f727fac83f8bbe7fd88d93bb.js"></script> <script src="//a.academia-assets.com/assets/webpack_bundles/webpack_libraries_and_infrequently_changed.wjs-bundle-bad3fa1257b860f4633ff1db966aa3ae7dfe1980708675f3e2488742c1a0d941.js"></script> <script src="//a.academia-assets.com/assets/webpack_bundles/core_webpack.wjs-bundle-34a3460a4873b743570e35df99c7839e3ddd9a3d06ef96c9fe38311a96a8a24e.js"></script> <script src="//a.academia-assets.com/assets/webpack_bundles/sentry.wjs-bundle-5fe03fddca915c8ba0f7edbe64c194308e8ce5abaed7bffe1255ff37549c4808.js"></script> <script> jade = window.jade || {}; jade.helpers = window.$h; jade._ = window._; </script>  <script id="tag-manager-head-root">(function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start': new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0], j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src= 'https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f); })(window,document,'script','dataLayer_old','GTM-5G9JF7Z');</script>  <script> window.gptadslots = []; window.googletag = window.googletag || {}; window.googletag.cmd = window.googletag.cmd || []; </script> <script type="text/javascript"> // TODO(jacob): This should be defined, may be rare load order problem. // Checking if null is just a quick fix, will default to en if unset. // Better fix is to run this immedietely after I18n is set. if (window.I18n != null) { I18n.defaultLocale = "en"; I18n.locale = "en"; I18n.fallbacks = true; } </script> <link rel="canonical" href="https://independent.academia.edu/HarePradhan" /> </head>   <body class='c-profiles/works a-summary logged_out'>  <div id="fb-root"></div><script>window.fbAsyncInit = function() { FB.init({ appId: "2369844204", version: "v8.0", status: true, cookie: true, xfbml: true }); // Additional initialization code. if (window.InitFacebook) { // facebook.ts already loaded, set it up. window.InitFacebook(); } else { // Set a flag for facebook.ts to find when it loads. window.academiaAuthReadyFacebook = true; } };</script><script>window.fbAsyncLoad = function() { // Protection against double calling of this function if (window.FB) { return; } (function(d, s, id){ var js, fjs = d.getElementsByTagName(s)[0]; if (d.getElementById(id)) {return;} js = d.createElement(s); js.id = id; js.src = "//connect.facebook.net/en_US/sdk.js"; fjs.parentNode.insertBefore(js, fjs); }(document, 'script', 'facebook-jssdk')); } if (!window.defer_facebook) { // Autoload if not deferred window.fbAsyncLoad(); } else { // Defer loading by 5 seconds setTimeout(function() { window.fbAsyncLoad(); }, 5000); }</script> <div id="google-root"></div><script>window.loadGoogle = function() { if (window.InitGoogle) { // google.ts already loaded, set it up. window.InitGoogle("331998490334-rsn3chp12mbkiqhl6e7lu2q0mlbu0f1b"); } else { // Set a flag for google.ts to use when it loads. window.GoogleClientID = "331998490334-rsn3chp12mbkiqhl6e7lu2q0mlbu0f1b"; } };</script><script>window.googleAsyncLoad = function() { // Protection against double calling of this function (function(d) { var js; var id = 'google-jssdk'; var ref = d.getElementsByTagName('script')[0]; if (d.getElementById(id)) { return; } js = d.createElement('script'); js.id = id; js.async = true; js.onload = loadGoogle; js.src = "https://accounts.google.com/gsi/client" ref.parentNode.insertBefore(js, ref); }(document)); } if (!window.defer_google) { // Autoload if not deferred window.googleAsyncLoad(); } else { // Defer loading by 5 seconds setTimeout(function() { window.googleAsyncLoad(); }, 5000); }</script> <div id="tag-manager-body-root">  <noscript><iframe src="https://www.googletagmanager.com/ns.html?id=GTM-5G9JF7Z" height="0" width="0" style="display:none;visibility:hidden"></iframe></noscript>   <script> window.addEventListener('load', function() { if (document.querySelector('input[name="commit"]')) { document.querySelector('input[name="commit"]').addEventListener('click', function() { gtag('event', 'click', { event_category: 'button', event_label: 'Log In' }) }) } }); </script> </div> <script>var _comscore = _comscore || []; _comscore.push({ c1: "2", c2: "26766707" }); (function() { var s = document.createElement("script"), el = document.getElementsByTagName("script")[0]; s.async = true; s.src = (document.location.protocol == "https:" ? "https://sb" : "http://b") + ".scorecardresearch.com/beacon.js"; el.parentNode.insertBefore(s, el); })();</script><img src="https://sb.scorecardresearch.com/p?c1=2&c2=26766707&cv=2.0&cj=1" style="position: absolute; visibility: hidden" /> <div id='react-modal'></div> <div class='DesignSystem'> <a class='u-showOnFocus' href='#site'> Skip to main content </a> </div> <div id="upgrade_ie_banner" style="display: none;"><p>Academia.edu no longer supports Internet Explorer.</p><p>To browse Academia.edu and the wider internet faster and more securely, please take a few seconds to <a href="https://www.academia.edu/upgrade-browser">upgrade your browser</a>.</p></div><script>// Show this banner for all versions of IE if (!!window.MSInputMethodContext || /(MSIE)/.test(navigator.userAgent)) { document.getElementById('upgrade_ie_banner').style.display = 'block'; }</script> <div class="DesignSystem bootstrap ShrinkableNav"><div class="navbar navbar-default main-header"><div class="container-wrapper" id="main-header-container"><div class="container"><div class="navbar-header"><div class="nav-left-wrapper u-mt0x"><div class="nav-logo"><a data-main-header-link-target="logo_home" href="https://www.academia.edu/"><img class="visible-xs-inline-block" style="height: 24px;" alt="Academia.edu" src="//a.academia-assets.com/images/academia-logo-redesign-2015-A.svg" width="24" height="24" /><img width="145.2" height="18" class="hidden-xs" style="height: 24px;" alt="Academia.edu" src="//a.academia-assets.com/images/academia-logo-redesign-2015.svg" /></a></div><div class="nav-search"><div class="SiteSearch-wrapper select2-no-default-pills"><form class="js-SiteSearch-form DesignSystem" action="https://www.academia.edu/search" accept-charset="UTF-8" method="get"><input name="utf8" type="hidden" value="✓" autocomplete="off" /><i class="SiteSearch-icon fa fa-search u-fw700 u-positionAbsolute u-tcGrayDark"></i><input class="js-SiteSearch-form-input SiteSearch-form-input form-control" data-main-header-click-target="search_input" name="q" placeholder="Search" type="text" value="" /></form></div></div></div><div class="nav-right-wrapper pull-right"><ul class="NavLinks js-main-nav list-unstyled"><li class="NavLinks-link"><a class="js-header-login-url Button Button--inverseGray Button--sm u-mb4x" id="nav_log_in" rel="nofollow" href="https://www.academia.edu/login">Log In</a></li><li class="NavLinks-link u-p0x"><a class="Button Button--inverseGray Button--sm u-mb4x" rel="nofollow" href="https://www.academia.edu/signup">Sign Up</a></li></ul><button class="hidden-lg hidden-md hidden-sm u-ml4x navbar-toggle collapsed" data-target=".js-mobile-header-links" data-toggle="collapse" type="button"><span class="icon-bar"></span><span class="icon-bar"></span><span class="icon-bar"></span></button></div></div><div class="collapse navbar-collapse js-mobile-header-links"><ul class="nav navbar-nav"><li class="u-borderColorGrayLight u-borderBottom1"><a rel="nofollow" href="https://www.academia.edu/login">Log In</a></li><li class="u-borderColorGrayLight u-borderBottom1"><a rel="nofollow" href="https://www.academia.edu/signup">Sign Up</a></li><li class="u-borderColorGrayLight u-borderBottom1 js-mobile-nav-expand-trigger"><a href="#">more&nbsp<span class="caret"></span></a></li><li><ul class="js-mobile-nav-expand-section nav navbar-nav u-m0x collapse"><li class="u-borderColorGrayLight u-borderBottom1"><a rel="false" href="https://www.academia.edu/about">About</a></li><li class="u-borderColorGrayLight u-borderBottom1"><a rel="nofollow" href="https://www.academia.edu/press">Press</a></li><li class="u-borderColorGrayLight u-borderBottom1"><a rel="nofollow" href="https://medium.com/@academia">Blog</a></li><li class="u-borderColorGrayLight u-borderBottom1"><a rel="false" href="https://www.academia.edu/documents">Papers</a></li><li class="u-borderColorGrayLight u-borderBottom1"><a rel="nofollow" href="https://www.academia.edu/terms">Terms</a></li><li class="u-borderColorGrayLight u-borderBottom1"><a rel="nofollow" href="https://www.academia.edu/privacy">Privacy</a></li><li class="u-borderColorGrayLight u-borderBottom1"><a rel="nofollow" href="https://www.academia.edu/copyright">Copyright</a></li><li class="u-borderColorGrayLight u-borderBottom1"><a rel="nofollow" href="https://www.academia.edu/hiring"><i class="fa fa-briefcase"></i> We're Hiring!</a></li><li class="u-borderColorGrayLight u-borderBottom1"><a rel="nofollow" href="https://support.academia.edu/"><i class="fa fa-question-circle"></i> Help Center</a></li><li class="js-mobile-nav-collapse-trigger u-borderColorGrayLight u-borderBottom1 dropup" style="display:none"><a href="#">less&nbsp<span class="caret"></span></a></li></ul></li></ul></div></div></div><script>(function(){ var $moreLink = $(".js-mobile-nav-expand-trigger"); var $lessLink = $(".js-mobile-nav-collapse-trigger"); var $section = $('.js-mobile-nav-expand-section'); $moreLink.click(function(ev){ ev.preventDefault(); $moreLink.hide(); $lessLink.show(); $section.collapse('show'); }); $lessLink.click(function(ev){ ev.preventDefault(); $moreLink.show(); $lessLink.hide(); $section.collapse('hide'); }); })() if ($a.is_logged_in() || false) { new Aedu.NavigationController({ el: '.js-main-nav', showHighlightedNotification: false }); } else { $(".js-header-login-url").attr("href", $a.loginUrlWithRedirect()); } Aedu.autocompleteSearch = new AutocompleteSearch({el: '.js-SiteSearch-form'});</script></div></div> <div id='site' class='fixed'> <div id="content" class="clearfix"> <script>document.addEventListener('DOMContentLoaded', function(){ var $dismissible = $(".dismissible_banner"); $dismissible.click(function(ev) { $dismissible.hide(); }); });</script> <script src="//a.academia-assets.com/assets/webpack_bundles/profile.wjs-bundle-d50db999ccdab527b1e040b4cc8af62d4a8254a0385f5004e234635ba055442a.js" defer="defer"></script><script>Aedu.rankings = { showPaperRankingsLink: false } $viewedUser = Aedu.User.set_viewed( {"id":36693995,"first_name":"Hare","middle_initials":"","last_name":"Pradhan","page_name":"HarePradhan","domain_name":"independent","created_at":"2015-10-21T11:07:05.415-07:00","display_name":"Hare Pradhan","url":"https://independent.academia.edu/HarePradhan","photo":"/images/s65_no_pic.png","has_photo":false,"is_analytics_public":false,"interests":[]} ); if ($a.is_logged_in() && $viewedUser.is_current_user()) { $('body').addClass('profile-viewed-by-owner'); } $socialProfiles = []</script><div id="js-react-on-rails-context" style="display:none" data-rails-context="{"inMailer":false,"i18nLocale":"en","i18nDefaultLocale":"en","href":"https://independent.academia.edu/HarePradhan","location":"/HarePradhan","scheme":"https","host":"independent.academia.edu","port":null,"pathname":"/HarePradhan","search":null,"httpAcceptLanguage":null,"serverSide":false}"></div> <div class="js-react-on-rails-component" style="display:none" data-component-name="ProfileCheckPaperUpdate" data-props="{}" data-trace="false" data-dom-id="ProfileCheckPaperUpdate-react-component-f9ebf56a-0d76-4c8f-a279-4c9379024411"></div> <div id="ProfileCheckPaperUpdate-react-component-f9ebf56a-0d76-4c8f-a279-4c9379024411"></div> <div class="DesignSystem"><div class="onsite-ping" id="onsite-ping"></div></div><div class="profile-user-info DesignSystem"><div class="social-profile-container"><div class="left-panel-container"><div class="user-info-component-wrapper"><div class="user-summary-cta-container"><div class="user-summary-container"><div class="social-profile-avatar-container"><img class="profile-avatar u-positionAbsolute" border="0" alt="" src="//a.academia-assets.com/images/s200_no_pic.png" /></div><div class="title-container"><h1 class="ds2-5-heading-sans-serif-sm">Hare Pradhan</h1><div class="affiliations-container fake-truncate js-profile-affiliations"></div></div></div><div class="sidebar-cta-container"><button class="ds2-5-button hidden profile-cta-button grow js-profile-follow-button" data-broccoli-component="user-info.follow-button" data-click-track="profile-user-info-follow-button" data-follow-user-fname="Hare" data-follow-user-id="36693995" data-follow-user-source="profile_button" data-has-google="false"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">add</span>Follow</button><button class="ds2-5-button hidden profile-cta-button grow js-profile-unfollow-button" data-broccoli-component="user-info.unfollow-button" data-click-track="profile-user-info-unfollow-button" data-unfollow-user-id="36693995"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">done</span>Following</button></div></div><div class="user-stats-container"><a><div class="stat-container js-profile-followers"><p class="label">Followers</p><p class="data">16</p></div></a><a><div class="stat-container js-profile-followees" data-broccoli-component="user-info.followees-count" data-click-track="profile-expand-user-info-following"><p class="label">Following</p><p class="data">9</p></div></a><a><div class="stat-container js-profile-coauthors" data-broccoli-component="user-info.coauthors-count" data-click-track="profile-expand-user-info-coauthors"><p class="label">Co-authors</p><p class="data">9</p></div></a><span><div class="stat-container"><p class="label"><span class="js-profile-total-view-text">Public Views</span></p><p class="data"><span class="js-profile-view-count"></span></p></div></span></div></div></div><div class="right-panel-container"><div class="user-content-wrapper"><div class="uploads-container" id="social-redesign-work-container"><div class="upload-header"><h2 class="ds2-5-heading-sans-serif-xs">Uploads</h2></div><div class="documents-container backbone-social-profile-documents" style="width: 100%;"><div class="u-taCenter"></div><div class="profile--tab_content_container js-tab-pane tab-pane active" id="all"><div class="profile--tab_heading_container js-section-heading" data-section="Papers" id="Papers"><h3 class="profile--tab_heading_container">Papers by Hare Pradhan</h3></div><div class="js-work-strip profile--work_container" data-work-id="17119392"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/17119392/Dandapat_S_Pradhan_H_K_and_Mohanty_G_C_1994_Anti_idiotype_antibodies_to_Marek_s_disease_associated_tumor_surface_antigens_in_protection_against_Marek_s_disease_Vet_Immunol_Immunopathol_40_353_366"><img alt="Research paper thumbnail of Dandapat, S., Pradhan, H.K. and Mohanty, G.C. (1994). Anti-idiotype antibodies to Marek’s disease – associated tumor surface antigens in protection against Marek’s disease. Vet. Immunol. Immunopathol., 40: 353-366" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/17119392/Dandapat_S_Pradhan_H_K_and_Mohanty_G_C_1994_Anti_idiotype_antibodies_to_Marek_s_disease_associated_tumor_surface_antigens_in_protection_against_Marek_s_disease_Vet_Immunol_Immunopathol_40_353_366">Dandapat, S., Pradhan, H.K. and Mohanty, G.C. (1994). Anti-idiotype antibodies to Marek’s disease – associated tumor surface antigens in protection against Marek’s disease. Vet. Immunol. Immunopathol., 40: 353-366</a></div><div class="wp-workCard_item"><span>Veterinary Immunology and Immunopathology</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="17119392"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="17119392"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 17119392; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=17119392]").text(description); $(".js-view-count[data-work-id=17119392]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 17119392; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='17119392']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 17119392, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=17119392]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":17119392,"title":"Dandapat, S., Pradhan, H.K. and Mohanty, G.C. (1994). Anti-idiotype antibodies to Marek’s disease – associated tumor surface antigens in protection against Marek’s disease. Vet. Immunol. Immunopathol., 40: 353-366","translated_title":"","metadata":{"publication_name":"Veterinary Immunology and Immunopathology"},"translated_abstract":null,"internal_url":"https://www.academia.edu/17119392/Dandapat_S_Pradhan_H_K_and_Mohanty_G_C_1994_Anti_idiotype_antibodies_to_Marek_s_disease_associated_tumor_surface_antigens_in_protection_against_Marek_s_disease_Vet_Immunol_Immunopathol_40_353_366","translated_internal_url":"","created_at":"2015-10-21T11:12:55.511-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":36693995,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":7717087,"work_id":17119392,"tagging_user_id":36693995,"tagged_user_id":12271862,"co_author_invite_id":null,"email":"g***y@gmail.com","display_order":0,"name":"Gagan Mohanty","title":"Dandapat, S., Pradhan, H.K. and Mohanty, G.C. (1994). Anti-idiotype antibodies to Marek’s disease – associated tumor surface antigens in protection against Marek’s disease. Vet. Immunol. Immunopathol., 40: 353-366"},{"id":7717092,"work_id":17119392,"tagging_user_id":36693995,"tagged_user_id":null,"co_author_invite_id":1723478,"email":"s***t@hotmail.com","display_order":4194304,"name":"S. Dandapat","title":"Dandapat, S., Pradhan, H.K. and Mohanty, G.C. (1994). Anti-idiotype antibodies to Marek’s disease – associated tumor surface antigens in protection against Marek’s disease. Vet. Immunol. Immunopathol., 40: 353-366"}],"downloadable_attachments":[],"slug":"Dandapat_S_Pradhan_H_K_and_Mohanty_G_C_1994_Anti_idiotype_antibodies_to_Marek_s_disease_associated_tumor_surface_antigens_in_protection_against_Marek_s_disease_Vet_Immunol_Immunopathol_40_353_366","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":36693995,"first_name":"Hare","middle_initials":"","last_name":"Pradhan","page_name":"HarePradhan","domain_name":"independent","created_at":"2015-10-21T11:07:05.415-07:00","display_name":"Hare Pradhan","url":"https://independent.academia.edu/HarePradhan"},"attachments":[],"research_interests":[{"id":173,"name":"Zoology","url":"https://www.academia.edu/Documents/in/Zoology"},{"id":644860,"name":"Veterinary Sciences","url":"https://www.academia.edu/Documents/in/Veterinary_Sciences"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="17119391"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/17119391/Genetic_analysis_of_indian_bovine_viral_diarrhea_virus_1_isolates_in_N_pro_and_entire_gene_region_coding_structural_proteins"><img alt="Research paper thumbnail of Genetic analysis of indian bovine viral diarrhea virus 1 isolates in N(pro) and entire gene region coding structural proteins" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/17119391/Genetic_analysis_of_indian_bovine_viral_diarrhea_virus_1_isolates_in_N_pro_and_entire_gene_region_coding_structural_proteins">Genetic analysis of indian bovine viral diarrhea virus 1 isolates in N(pro) and entire gene region coding structural proteins</a></div><div class="wp-workCard_item"><span>Acta virologica</span><span>, 2006</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Three Indian Bovine viral diarrhea virus 1 (BVDV-1) isolates were analyzed at genetic level in N(...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Three Indian Bovine viral diarrhea virus 1 (BVDV-1) isolates were analyzed at genetic level in N(pro) (viral autoprotease) and entire gene region coding structural proteins, namely capsid (C) protein, E(rns), and envelope proteins E1 and E2. All these isolates were found to be of b subtype based on the entire 504 nt region of N(pro) and 1119 nt region of E2. However, in comparison with other isolates of this subtype, they were allocated inside the BVDV-1 subtype b cluster to a separate clade with a longer distance. Of six cysteine residues in N(pro) only three were totally conserved in all three isolates. The isolates showed 94.9-99.3% and 92.2-99.0% identities for the entire C-E2 gene region at nucleotide and amino acid levels, respectively. The lowest identity values (88.5-91.7%) were observed for E2 amino acid sequences. The identity of the isolates with Osloss, a reference BVDV-1 subtype b strain, was in the range of 82.1-89.9% for nucleotide and 78.6-89.2% for amino acid sequen...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="17119391"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="17119391"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 17119391; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=17119391]").text(description); $(".js-view-count[data-work-id=17119391]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 17119391; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='17119391']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 17119391, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=17119391]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":17119391,"title":"Genetic analysis of indian bovine viral diarrhea virus 1 isolates in N(pro) and entire gene region coding structural proteins","translated_title":"","metadata":{"abstract":"Three Indian Bovine viral diarrhea virus 1 (BVDV-1) isolates were analyzed at genetic level in N(pro) (viral autoprotease) and entire gene region coding structural proteins, namely capsid (C) protein, E(rns), and envelope proteins E1 and E2. All these isolates were found to be of b subtype based on the entire 504 nt region of N(pro) and 1119 nt region of E2. However, in comparison with other isolates of this subtype, they were allocated inside the BVDV-1 subtype b cluster to a separate clade with a longer distance. Of six cysteine residues in N(pro) only three were totally conserved in all three isolates. The isolates showed 94.9-99.3% and 92.2-99.0% identities for the entire C-E2 gene region at nucleotide and amino acid levels, respectively. The lowest identity values (88.5-91.7%) were observed for E2 amino acid sequences. The identity of the isolates with Osloss, a reference BVDV-1 subtype b strain, was in the range of 82.1-89.9% for nucleotide and 78.6-89.2% for amino acid sequen...","publication_date":{"day":null,"month":null,"year":2006,"errors":{}},"publication_name":"Acta virologica"},"translated_abstract":"Three Indian Bovine viral diarrhea virus 1 (BVDV-1) isolates were analyzed at genetic level in N(pro) (viral autoprotease) and entire gene region coding structural proteins, namely capsid (C) protein, E(rns), and envelope proteins E1 and E2. All these isolates were found to be of b subtype based on the entire 504 nt region of N(pro) and 1119 nt region of E2. However, in comparison with other isolates of this subtype, they were allocated inside the BVDV-1 subtype b cluster to a separate clade with a longer distance. Of six cysteine residues in N(pro) only three were totally conserved in all three isolates. The isolates showed 94.9-99.3% and 92.2-99.0% identities for the entire C-E2 gene region at nucleotide and amino acid levels, respectively. The lowest identity values (88.5-91.7%) were observed for E2 amino acid sequences. The identity of the isolates with Osloss, a reference BVDV-1 subtype b strain, was in the range of 82.1-89.9% for nucleotide and 78.6-89.2% for amino acid sequen...","internal_url":"https://www.academia.edu/17119391/Genetic_analysis_of_indian_bovine_viral_diarrhea_virus_1_isolates_in_N_pro_and_entire_gene_region_coding_structural_proteins","translated_internal_url":"","created_at":"2015-10-21T11:12:55.425-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":36693995,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":7717033,"work_id":17119391,"tagging_user_id":36693995,"tagged_user_id":50823026,"co_author_invite_id":616470,"email":"m***r@rediffmail.com","display_order":0,"name":"Niranjan Mishra","title":"Genetic analysis of indian bovine viral diarrhea virus 1 isolates in N(pro) and entire gene region coding structural proteins"},{"id":7717037,"work_id":17119391,"tagging_user_id":36693995,"tagged_user_id":1175172,"co_author_invite_id":null,"email":"s***9@gmail.com","display_order":4194304,"name":"Shreyas Pitale","title":"Genetic analysis of indian bovine viral diarrhea virus 1 isolates in N(pro) and entire gene region coding structural proteins"},{"id":7717095,"work_id":17119391,"tagging_user_id":36693995,"tagged_user_id":8516907,"co_author_invite_id":null,"email":"p***n@ignou.ac.in","display_order":6291456,"name":"PRAVEEN KUMAR JAIN","title":"Genetic analysis of indian bovine viral diarrhea virus 1 isolates in N(pro) and entire gene region coding structural proteins"},{"id":7717099,"work_id":17119391,"tagging_user_id":36693995,"tagged_user_id":33035758,"co_author_invite_id":null,"email":"v***k@uvm.sk","display_order":7340032,"name":"Stefan Vilcek","title":"Genetic analysis of indian bovine viral diarrhea virus 1 isolates in N(pro) and entire gene region coding structural proteins"}],"downloadable_attachments":[],"slug":"Genetic_analysis_of_indian_bovine_viral_diarrhea_virus_1_isolates_in_N_pro_and_entire_gene_region_coding_structural_proteins","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":36693995,"first_name":"Hare","middle_initials":"","last_name":"Pradhan","page_name":"HarePradhan","domain_name":"independent","created_at":"2015-10-21T11:07:05.415-07:00","display_name":"Hare Pradhan","url":"https://independent.academia.edu/HarePradhan"},"attachments":[],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology"},{"id":6947,"name":"Medical Microbiology","url":"https://www.academia.edu/Documents/in/Medical_Microbiology"},{"id":74780,"name":"Mutation","url":"https://www.academia.edu/Documents/in/Mutation"},{"id":260829,"name":"Cattle","url":"https://www.academia.edu/Documents/in/Cattle"},{"id":809881,"name":"Amino Acid Sequence","url":"https://www.academia.edu/Documents/in/Amino_Acid_Sequence"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="17119390"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/17119390/Bioterrorism_How_prepared_are_we"><img alt="Research paper thumbnail of Bioterrorism: How prepared are we?" class="work-thumbnail" src="https://attachments.academia-assets.com/42316763/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/17119390/Bioterrorism_How_prepared_are_we">Bioterrorism: How prepared are we?</a></div><div class="wp-workCard_item"><span>Indian Journal of Medical Specialities</span><span>, 2012</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="d46fbf84982c97a5e6991983db389909" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":42316763,"asset_id":17119390,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/42316763/download_file?st=MTczMzE5NzgyMSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="17119390"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="17119390"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 17119390; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=17119390]").text(description); $(".js-view-count[data-work-id=17119390]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 17119390; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='17119390']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 17119390, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "d46fbf84982c97a5e6991983db389909" } } $('.js-work-strip[data-work-id=17119390]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":17119390,"title":"Bioterrorism: How prepared are we?","translated_title":"","metadata":{"publication_date":{"day":null,"month":null,"year":2012,"errors":{}},"publication_name":"Indian Journal of Medical Specialities"},"translated_abstract":null,"internal_url":"https://www.academia.edu/17119390/Bioterrorism_How_prepared_are_we","translated_internal_url":"","created_at":"2015-10-21T11:12:55.333-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":36693995,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":7717022,"work_id":17119390,"tagging_user_id":36693995,"tagged_user_id":null,"co_author_invite_id":124581,"email":"p***b@gmail.com","display_order":0,"name":"B. Pattnaik","title":"Bioterrorism: How prepared are we?"},{"id":7717096,"work_id":17119390,"tagging_user_id":36693995,"tagged_user_id":319840673,"co_author_invite_id":320366,"email":"d***r@gmail.com","display_order":4194304,"name":"Sitanshu Kar","title":"Bioterrorism: How prepared are we?"},{"id":7717097,"work_id":17119390,"tagging_user_id":36693995,"tagged_user_id":null,"co_author_invite_id":1723480,"email":"d***a@miami.edu","display_order":6291456,"name":"D. Shalala","title":"Bioterrorism: How prepared are we?"}],"downloadable_attachments":[{"id":42316763,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/42316763/thumbnails/1.jpg","file_name":"Bioterrorism_How_prepared_are_we20160207-11024-1s8pyac.pdf","download_url":"https://www.academia.edu/attachments/42316763/download_file?st=MTczMzE5NzgyMSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Bioterrorism_How_prepared_are_we.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/42316763/Bioterrorism_How_prepared_are_we20160207-11024-1s8pyac-libre.pdf?1454868701=\u0026response-content-disposition=attachment%3B+filename%3DBioterrorism_How_prepared_are_we.pdf\u0026Expires=1733201421\u0026Signature=YIXy-d3Zfoo9pqL8t~iwvHH1Y2pAkhisovdR9-JvfIqWP3foH~VzJyDKVjGy3WwuoG1v4GdsTTBNq3ZJQhxtk9ny36anzdggG3wB5-askOaVLn8cHxVmiFJACwTrTdYaQsJlisWHAdRdArV8TKGo0q7fWxBHXsg8DhNjDAO7RaxW9AZ2lb~wb7~O4wZEbs6oSueBWvGLuBgAb47bdXnMC9A6cqrFpM47ZCSC1Fxrzzfgl2Ym80FP0IotR-UmdR6y6u7FWNDhhjYAnnF5Zk4sGJwb1qlzGjIceEuois2UhHXgMVzLAr6VMEtBeI2MCoNsVUk4cUP7SqOD2KYKuNN7Gw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Bioterrorism_How_prepared_are_we","translated_slug":"","page_count":6,"language":"en","content_type":"Work","owner":{"id":36693995,"first_name":"Hare","middle_initials":"","last_name":"Pradhan","page_name":"HarePradhan","domain_name":"independent","created_at":"2015-10-21T11:07:05.415-07:00","display_name":"Hare Pradhan","url":"https://independent.academia.edu/HarePradhan"},"attachments":[{"id":42316763,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/42316763/thumbnails/1.jpg","file_name":"Bioterrorism_How_prepared_are_we20160207-11024-1s8pyac.pdf","download_url":"https://www.academia.edu/attachments/42316763/download_file?st=MTczMzE5NzgyMSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Bioterrorism_How_prepared_are_we.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/42316763/Bioterrorism_How_prepared_are_we20160207-11024-1s8pyac-libre.pdf?1454868701=\u0026response-content-disposition=attachment%3B+filename%3DBioterrorism_How_prepared_are_we.pdf\u0026Expires=1733201421\u0026Signature=YIXy-d3Zfoo9pqL8t~iwvHH1Y2pAkhisovdR9-JvfIqWP3foH~VzJyDKVjGy3WwuoG1v4GdsTTBNq3ZJQhxtk9ny36anzdggG3wB5-askOaVLn8cHxVmiFJACwTrTdYaQsJlisWHAdRdArV8TKGo0q7fWxBHXsg8DhNjDAO7RaxW9AZ2lb~wb7~O4wZEbs6oSueBWvGLuBgAb47bdXnMC9A6cqrFpM47ZCSC1Fxrzzfgl2Ym80FP0IotR-UmdR6y6u7FWNDhhjYAnnF5Zk4sGJwb1qlzGjIceEuois2UhHXgMVzLAr6VMEtBeI2MCoNsVUk4cUP7SqOD2KYKuNN7Gw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":1697,"name":"Violence","url":"https://www.academia.edu/Documents/in/Violence"},{"id":6947,"name":"Medical Microbiology","url":"https://www.academia.edu/Documents/in/Medical_Microbiology"},{"id":16288,"name":"Public Health","url":"https://www.academia.edu/Documents/in/Public_Health"},{"id":17969,"name":"Health Policy","url":"https://www.academia.edu/Documents/in/Health_Policy"},{"id":40574,"name":"Emerging Infectious Diseases","url":"https://www.academia.edu/Documents/in/Emerging_Infectious_Diseases"},{"id":98134,"name":"United States","url":"https://www.academia.edu/Documents/in/United_States"},{"id":244814,"name":"Clinical Sciences","url":"https://www.academia.edu/Documents/in/Clinical_Sciences"},{"id":332907,"name":"Biological Warfare","url":"https://www.academia.edu/Documents/in/Biological_Warfare"},{"id":410370,"name":"Public health systems and services research","url":"https://www.academia.edu/Documents/in/Public_health_systems_and_services_research-1"}],"urls":[{"id":6522411,"url":"https://www.researchgate.net/profile/Sitanshu_Kar/publication/272717872_Bioterrorism_How_prepared_are_we/links/54f69f1c0cf21d8b8a5cfb6d.pdf"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="17119389"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/17119389/Histamine_in_Foods_Its_Safety_and_Human_Health_Implications"><img alt="Research paper thumbnail of Histamine in Foods: Its Safety and Human Health Implications" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/17119389/Histamine_in_Foods_Its_Safety_and_Human_Health_Implications">Histamine in Foods: Its Safety and Human Health Implications</a></div><div class="wp-workCard_item"><span>Journal of Food Science and Technology Nepal</span><span>, 2014</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="17119389"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="17119389"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 17119389; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=17119389]").text(description); $(".js-view-count[data-work-id=17119389]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 17119389; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='17119389']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 17119389, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=17119389]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":17119389,"title":"Histamine in Foods: Its Safety and Human Health Implications","translated_title":"","metadata":{"publication_date":{"day":null,"month":null,"year":2014,"errors":{}},"publication_name":"Journal of Food Science and Technology Nepal"},"translated_abstract":null,"internal_url":"https://www.academia.edu/17119389/Histamine_in_Foods_Its_Safety_and_Human_Health_Implications","translated_internal_url":"","created_at":"2015-10-21T11:12:55.253-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":36693995,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":7717102,"work_id":17119389,"tagging_user_id":36693995,"tagged_user_id":4842777,"co_author_invite_id":null,"email":"r***6@gmail.com","display_order":0,"name":"Krishna Rai","title":"Histamine in Foods: Its Safety and Human Health Implications"}],"downloadable_attachments":[],"slug":"Histamine_in_Foods_Its_Safety_and_Human_Health_Implications","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":36693995,"first_name":"Hare","middle_initials":"","last_name":"Pradhan","page_name":"HarePradhan","domain_name":"independent","created_at":"2015-10-21T11:07:05.415-07:00","display_name":"Hare Pradhan","url":"https://independent.academia.edu/HarePradhan"},"attachments":[],"research_interests":[{"id":49161,"name":"Safety","url":"https://www.academia.edu/Documents/in/Safety"},{"id":51569,"name":"Histamine","url":"https://www.academia.edu/Documents/in/Histamine"},{"id":65881,"name":"FISH","url":"https://www.academia.edu/Documents/in/FISH"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="17119388"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/17119388/Genetic_and_antigenic_characterization_of_bovine_viral_diarrhea_virus_type_2_isolated_from_Indian_goats_Capra_hircus_"><img alt="Research paper thumbnail of Genetic and antigenic characterization of bovine viral diarrhea virus type 2 isolated from Indian goats (Capra hircus)" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/17119388/Genetic_and_antigenic_characterization_of_bovine_viral_diarrhea_virus_type_2_isolated_from_Indian_goats_Capra_hircus_">Genetic and antigenic characterization of bovine viral diarrhea virus type 2 isolated from Indian goats (Capra hircus)</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/HarePradhan">Hare Pradhan</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/ATiwari1">A. Tiwari</a>, and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/ChakradharTosh">Chakradhar Tosh</a></span></div><div class="wp-workCard_item"><span>Veterinary Microbiology</span><span>, 2007</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Recent studies have shown that bovine viral diarrhea virus (BVDV) type 1 is widely prevalent in I...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Recent studies have shown that bovine viral diarrhea virus (BVDV) type 1 is widely prevalent in Indian cattle. In a surveillance of randomly collected 562 blood samples from seven states during 2004-2006, BVDV type 2 was detected in two native Indian goats by nested reverse transcription polymerase chain reaction (nRT-PCR). The virus isolated from them was classified antigenically as BVDV 2 on the basis of virus neutralization test and reactivity with monoclonal antibodies. Phylogenetic analysis of three different genomic regions, 5&amp;#39; un-translated region (5&amp;#39; UTR), E(rns) structural coding region and NS5B nonstructural coding region typed Indian goat isolate as BVDV 2a having close similarity with strains from North America and Europe suggesting its probable introduction through trade. It was placed in a separate clade within the 2a branch having unique mutations in E(rns) and NS5B region. This is the first report of BVDV 2 in India and only second time recorded in goat species. The isolation of BVDV 2 from goat warrants intensive surveillance in cattle and sheep.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="17119388"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="17119388"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 17119388; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=17119388]").text(description); $(".js-view-count[data-work-id=17119388]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 17119388; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='17119388']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 17119388, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=17119388]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":17119388,"title":"Genetic and antigenic characterization of bovine viral diarrhea virus type 2 isolated from Indian goats (Capra hircus)","translated_title":"","metadata":{"abstract":"Recent studies have shown that bovine viral diarrhea virus (BVDV) type 1 is widely prevalent in Indian cattle. In a surveillance of randomly collected 562 blood samples from seven states during 2004-2006, BVDV type 2 was detected in two native Indian goats by nested reverse transcription polymerase chain reaction (nRT-PCR). The virus isolated from them was classified antigenically as BVDV 2 on the basis of virus neutralization test and reactivity with monoclonal antibodies. Phylogenetic analysis of three different genomic regions, 5\u0026amp;#39; un-translated region (5\u0026amp;#39; UTR), E(rns) structural coding region and NS5B nonstructural coding region typed Indian goat isolate as BVDV 2a having close similarity with strains from North America and Europe suggesting its probable introduction through trade. It was placed in a separate clade within the 2a branch having unique mutations in E(rns) and NS5B region. This is the first report of BVDV 2 in India and only second time recorded in goat species. The isolation of BVDV 2 from goat warrants intensive surveillance in cattle and sheep.","publication_date":{"day":null,"month":null,"year":2007,"errors":{}},"publication_name":"Veterinary Microbiology"},"translated_abstract":"Recent studies have shown that bovine viral diarrhea virus (BVDV) type 1 is widely prevalent in Indian cattle. In a surveillance of randomly collected 562 blood samples from seven states during 2004-2006, BVDV type 2 was detected in two native Indian goats by nested reverse transcription polymerase chain reaction (nRT-PCR). The virus isolated from them was classified antigenically as BVDV 2 on the basis of virus neutralization test and reactivity with monoclonal antibodies. Phylogenetic analysis of three different genomic regions, 5\u0026amp;#39; un-translated region (5\u0026amp;#39; UTR), E(rns) structural coding region and NS5B nonstructural coding region typed Indian goat isolate as BVDV 2a having close similarity with strains from North America and Europe suggesting its probable introduction through trade. It was placed in a separate clade within the 2a branch having unique mutations in E(rns) and NS5B region. This is the first report of BVDV 2 in India and only second time recorded in goat species. The isolation of BVDV 2 from goat warrants intensive surveillance in cattle and sheep.","internal_url":"https://www.academia.edu/17119388/Genetic_and_antigenic_characterization_of_bovine_viral_diarrhea_virus_type_2_isolated_from_Indian_goats_Capra_hircus_","translated_internal_url":"","created_at":"2015-10-21T11:12:55.157-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":36693995,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":7717034,"work_id":17119388,"tagging_user_id":36693995,"tagged_user_id":50823026,"co_author_invite_id":616470,"email":"m***r@rediffmail.com","display_order":0,"name":"Niranjan Mishra","title":"Genetic and antigenic characterization of bovine viral diarrhea virus type 2 isolated from Indian goats (Capra hircus)"},{"id":7717038,"work_id":17119388,"tagging_user_id":36693995,"tagged_user_id":1175172,"co_author_invite_id":null,"email":"s***9@gmail.com","display_order":4194304,"name":"Shreyas Pitale","title":"Genetic and antigenic characterization of bovine viral diarrhea virus type 2 isolated from Indian goats (Capra hircus)"},{"id":7717063,"work_id":17119388,"tagging_user_id":36693995,"tagged_user_id":null,"co_author_invite_id":1723468,"email":"k***r@rediffmail.com","display_order":6291456,"name":"K. Rajukumar","title":"Genetic and antigenic characterization of bovine viral diarrhea virus type 2 isolated from Indian goats (Capra hircus)"},{"id":7717066,"work_id":17119388,"tagging_user_id":36693995,"tagged_user_id":null,"co_author_invite_id":1723470,"email":"r***u@rediffmail.com","display_order":7340032,"name":"R. Dubey","title":"Genetic and antigenic characterization of bovine viral diarrhea virus type 2 isolated from Indian goats (Capra hircus)"},{"id":7717068,"work_id":17119388,"tagging_user_id":36693995,"tagged_user_id":36958264,"co_author_invite_id":1723471,"email":"a***3@yahoo.com","display_order":7864320,"name":"A. Tiwari","title":"Genetic and antigenic characterization of bovine viral diarrhea virus type 2 isolated from Indian goats (Capra hircus)"},{"id":7717094,"work_id":17119388,"tagging_user_id":36693995,"tagged_user_id":50718669,"co_author_invite_id":522492,"email":"c***h@gmail.com","display_order":8126464,"name":"Chakradhar Tosh","title":"Genetic and antigenic characterization of bovine viral diarrhea virus type 2 isolated from Indian goats (Capra hircus)"}],"downloadable_attachments":[],"slug":"Genetic_and_antigenic_characterization_of_bovine_viral_diarrhea_virus_type_2_isolated_from_Indian_goats_Capra_hircus_","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":36693995,"first_name":"Hare","middle_initials":"","last_name":"Pradhan","page_name":"HarePradhan","domain_name":"independent","created_at":"2015-10-21T11:07:05.415-07:00","display_name":"Hare Pradhan","url":"https://independent.academia.edu/HarePradhan"},"attachments":[],"research_interests":[{"id":156,"name":"Genetics","url":"https://www.academia.edu/Documents/in/Genetics"},{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology"},{"id":3037,"name":"Veterinary Microbiology","url":"https://www.academia.edu/Documents/in/Veterinary_Microbiology"},{"id":35414,"name":"Veterinary","url":"https://www.academia.edu/Documents/in/Veterinary"},{"id":43838,"name":"India","url":"https://www.academia.edu/Documents/in/India"},{"id":54433,"name":"Phylogeny","url":"https://www.academia.edu/Documents/in/Phylogeny"},{"id":103298,"name":"Blood sampling","url":"https://www.academia.edu/Documents/in/Blood_sampling"},{"id":147196,"name":"Monoclonal Antibodies","url":"https://www.academia.edu/Documents/in/Monoclonal_Antibodies"},{"id":158782,"name":"Phylogenetic analysis","url":"https://www.academia.edu/Documents/in/Phylogenetic_analysis"},{"id":252969,"name":"Goats","url":"https://www.academia.edu/Documents/in/Goats"},{"id":253469,"name":"North America","url":"https://www.academia.edu/Documents/in/North_America"},{"id":644860,"name":"Veterinary Sciences","url":"https://www.academia.edu/Documents/in/Veterinary_Sciences"},{"id":766014,"name":"Monoclonal Antibody","url":"https://www.academia.edu/Documents/in/Monoclonal_Antibody"},{"id":809881,"name":"Amino Acid Sequence","url":"https://www.academia.edu/Documents/in/Amino_Acid_Sequence"},{"id":1126122,"name":"Capra Hircus","url":"https://www.academia.edu/Documents/in/Capra_Hircus"},{"id":1709309,"name":"Bovine Viral Diarrhea Virus","url":"https://www.academia.edu/Documents/in/Bovine_Viral_Diarrhea_Virus"},{"id":2007837,"name":"Virus isolation","url":"https://www.academia.edu/Documents/in/Virus_isolation"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="17119387"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/17119387/Anti_idiotype_antibodies_to_Mareks_disease_associated_tumour_surface_antigen_in_protection_against_Mareks_disease"><img alt="Research paper thumbnail of Anti-idiotype antibodies to Marek's disease-associated tumour surface antigen in protection against Marek's disease" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/17119387/Anti_idiotype_antibodies_to_Mareks_disease_associated_tumour_surface_antigen_in_protection_against_Mareks_disease">Anti-idiotype antibodies to Marek's disease-associated tumour surface antigen in protection against Marek's disease</a></div><div class="wp-workCard_item"><span>Veterinary Immunology and Immunopathology</span><span>, 1994</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Marek&amp;#39;s disease-associated tumour surface antigen (MATSA) removed by enzymatic (papain) d...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Marek&amp;#39;s disease-associated tumour surface antigen (MATSA) removed by enzymatic (papain) digestion of Marek&amp;#39;s disease tumour cells was fractionated by gel filtration chromatography. The first peak (F1) was used to raise antibody in rabbits. Monoclonal antibody (RPH-6) directed against MATSA and the anti-F1 IgG were used as idiotypic antibodies to raise polyclonal anti-idiotype serum in heterologous hosts; rabbit and goat, respectively. The anti-idiotypes (anti-Id) were purified by affinity chromatography and characterized by competitive binding assay using immunofluorescent (IF) tests. Day-old white Leghorn chicks were immunized with anti-Id to MATSA (Group 1) or anti-Id to F1 (Group 3) and challenged with virulent Marek&amp;#39;s disease virus (MDV) on the tenth day post immunization. In positive control groups, the day-old chicks were inoculated with anti-BALB/c mouse globulin (Group 2) and anti-rabbit globulin (Group 4) and challenged with virulent MDV on the tenth day post inoculation. As compared with positive control groups, the vaccinated groups (1 and 3) had considerably lower level of MATSA positive cells during the post challenge observation period. The protection level against MD in the immunized groups was 66.6% (Group 1) and 86.6% (Group 3).</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="17119387"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="17119387"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 17119387; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=17119387]").text(description); $(".js-view-count[data-work-id=17119387]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 17119387; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='17119387']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 17119387, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=17119387]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":17119387,"title":"Anti-idiotype antibodies to Marek's disease-associated tumour surface antigen in protection against Marek's disease","translated_title":"","metadata":{"abstract":"Marek\u0026amp;#39;s disease-associated tumour surface antigen (MATSA) removed by enzymatic (papain) digestion of Marek\u0026amp;#39;s disease tumour cells was fractionated by gel filtration chromatography. The first peak (F1) was used to raise antibody in rabbits. Monoclonal antibody (RPH-6) directed against MATSA and the anti-F1 IgG were used as idiotypic antibodies to raise polyclonal anti-idiotype serum in heterologous hosts; rabbit and goat, respectively. The anti-idiotypes (anti-Id) were purified by affinity chromatography and characterized by competitive binding assay using immunofluorescent (IF) tests. Day-old white Leghorn chicks were immunized with anti-Id to MATSA (Group 1) or anti-Id to F1 (Group 3) and challenged with virulent Marek\u0026amp;#39;s disease virus (MDV) on the tenth day post immunization. In positive control groups, the day-old chicks were inoculated with anti-BALB/c mouse globulin (Group 2) and anti-rabbit globulin (Group 4) and challenged with virulent MDV on the tenth day post inoculation. As compared with positive control groups, the vaccinated groups (1 and 3) had considerably lower level of MATSA positive cells during the post challenge observation period. The protection level against MD in the immunized groups was 66.6% (Group 1) and 86.6% (Group 3).","publication_date":{"day":null,"month":null,"year":1994,"errors":{}},"publication_name":"Veterinary Immunology and Immunopathology"},"translated_abstract":"Marek\u0026amp;#39;s disease-associated tumour surface antigen (MATSA) removed by enzymatic (papain) digestion of Marek\u0026amp;#39;s disease tumour cells was fractionated by gel filtration chromatography. The first peak (F1) was used to raise antibody in rabbits. Monoclonal antibody (RPH-6) directed against MATSA and the anti-F1 IgG were used as idiotypic antibodies to raise polyclonal anti-idiotype serum in heterologous hosts; rabbit and goat, respectively. The anti-idiotypes (anti-Id) were purified by affinity chromatography and characterized by competitive binding assay using immunofluorescent (IF) tests. Day-old white Leghorn chicks were immunized with anti-Id to MATSA (Group 1) or anti-Id to F1 (Group 3) and challenged with virulent Marek\u0026amp;#39;s disease virus (MDV) on the tenth day post immunization. In positive control groups, the day-old chicks were inoculated with anti-BALB/c mouse globulin (Group 2) and anti-rabbit globulin (Group 4) and challenged with virulent MDV on the tenth day post inoculation. As compared with positive control groups, the vaccinated groups (1 and 3) had considerably lower level of MATSA positive cells during the post challenge observation period. The protection level against MD in the immunized groups was 66.6% (Group 1) and 86.6% (Group 3).","internal_url":"https://www.academia.edu/17119387/Anti_idiotype_antibodies_to_Mareks_disease_associated_tumour_surface_antigen_in_protection_against_Mareks_disease","translated_internal_url":"","created_at":"2015-10-21T11:12:55.070-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":36693995,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":7717084,"work_id":17119387,"tagging_user_id":36693995,"tagged_user_id":12271862,"co_author_invite_id":null,"email":"g***y@gmail.com","display_order":0,"name":"Gagan Mohanty","title":"Anti-idiotype antibodies to Marek's disease-associated tumour surface antigen in protection against Marek's disease"},{"id":7717091,"work_id":17119387,"tagging_user_id":36693995,"tagged_user_id":null,"co_author_invite_id":1723478,"email":"s***t@hotmail.com","display_order":4194304,"name":"S. Dandapat","title":"Anti-idiotype antibodies to Marek's disease-associated tumour surface antigen in protection against Marek's disease"}],"downloadable_attachments":[],"slug":"Anti_idiotype_antibodies_to_Mareks_disease_associated_tumour_surface_antigen_in_protection_against_Mareks_disease","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":36693995,"first_name":"Hare","middle_initials":"","last_name":"Pradhan","page_name":"HarePradhan","domain_name":"independent","created_at":"2015-10-21T11:07:05.415-07:00","display_name":"Hare Pradhan","url":"https://independent.academia.edu/HarePradhan"},"attachments":[],"research_interests":[{"id":173,"name":"Zoology","url":"https://www.academia.edu/Documents/in/Zoology"},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice"},{"id":132052,"name":"Marek Disease","url":"https://www.academia.edu/Documents/in/Marek_Disease"},{"id":252969,"name":"Goats","url":"https://www.academia.edu/Documents/in/Goats"},{"id":402759,"name":"Chickens","url":"https://www.academia.edu/Documents/in/Chickens"},{"id":644860,"name":"Veterinary Sciences","url":"https://www.academia.edu/Documents/in/Veterinary_Sciences"},{"id":782120,"name":"Veterinary Immunology","url":"https://www.academia.edu/Documents/in/Veterinary_Immunology"},{"id":788677,"name":"Rabbits","url":"https://www.academia.edu/Documents/in/Rabbits"},{"id":1716403,"name":"immunoglobulin G","url":"https://www.academia.edu/Documents/in/immunoglobulin_G"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="17119386"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/17119386/Immunopathology_of_Mareks_disease_in_quails_presence_of_antinuclear_antibody_and_immune_complex"><img alt="Research paper thumbnail of Immunopathology of Marek's disease in quails: presence of antinuclear antibody and immune complex" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/17119386/Immunopathology_of_Mareks_disease_in_quails_presence_of_antinuclear_antibody_and_immune_complex">Immunopathology of Marek's disease in quails: presence of antinuclear antibody and immune complex</a></div><div class="wp-workCard_item"><span>Veterinary Immunology and Immunopathology</span><span>, 1991</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Antinuclear antibody (ANA), a marker for autoimmune reactions, was detected in the sera of quails...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Antinuclear antibody (ANA), a marker for autoimmune reactions, was detected in the sera of quails with Marek&amp;#39;s disease (MD). The autoantibody was detected 3 weeks after infection in quails infected with chicken Marek&amp;#39;s disease virus and 4 weeks after infection in quails infected with quail Marek&amp;#39;s disease virus. The ANA titers were low and ranged from 10 to 40. A speckled type of nuclear fluorescence was the characteristic staining feature. In addition to the presence of ANA, immune complexes (IC) were also detected in the kidney glomeruli of quail infected with Marek&amp;#39;s disease virus. Initially about 25-30% of the glomeruli in the kidneys of infected quails had IC deposits. In subsequent periods, the amount of IC deposit and the number of glomeruli showing IC also increased considerably. The findings of the present study suggested autoimmunity may play a pathogenic role in MD.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="17119386"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="17119386"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 17119386; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=17119386]").text(description); $(".js-view-count[data-work-id=17119386]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 17119386; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='17119386']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 17119386, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=17119386]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":17119386,"title":"Immunopathology of Marek's disease in quails: presence of antinuclear antibody and immune complex","translated_title":"","metadata":{"abstract":"Antinuclear antibody (ANA), a marker for autoimmune reactions, was detected in the sera of quails with Marek\u0026amp;#39;s disease (MD). The autoantibody was detected 3 weeks after infection in quails infected with chicken Marek\u0026amp;#39;s disease virus and 4 weeks after infection in quails infected with quail Marek\u0026amp;#39;s disease virus. The ANA titers were low and ranged from 10 to 40. A speckled type of nuclear fluorescence was the characteristic staining feature. In addition to the presence of ANA, immune complexes (IC) were also detected in the kidney glomeruli of quail infected with Marek\u0026amp;#39;s disease virus. Initially about 25-30% of the glomeruli in the kidneys of infected quails had IC deposits. In subsequent periods, the amount of IC deposit and the number of glomeruli showing IC also increased considerably. The findings of the present study suggested autoimmunity may play a pathogenic role in MD.","publication_date":{"day":null,"month":null,"year":1991,"errors":{}},"publication_name":"Veterinary Immunology and Immunopathology"},"translated_abstract":"Antinuclear antibody (ANA), a marker for autoimmune reactions, was detected in the sera of quails with Marek\u0026amp;#39;s disease (MD). The autoantibody was detected 3 weeks after infection in quails infected with chicken Marek\u0026amp;#39;s disease virus and 4 weeks after infection in quails infected with quail Marek\u0026amp;#39;s disease virus. The ANA titers were low and ranged from 10 to 40. A speckled type of nuclear fluorescence was the characteristic staining feature. In addition to the presence of ANA, immune complexes (IC) were also detected in the kidney glomeruli of quail infected with Marek\u0026amp;#39;s disease virus. Initially about 25-30% of the glomeruli in the kidneys of infected quails had IC deposits. In subsequent periods, the amount of IC deposit and the number of glomeruli showing IC also increased considerably. The findings of the present study suggested autoimmunity may play a pathogenic role in MD.","internal_url":"https://www.academia.edu/17119386/Immunopathology_of_Mareks_disease_in_quails_presence_of_antinuclear_antibody_and_immune_complex","translated_internal_url":"","created_at":"2015-10-21T11:12:54.950-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":36693995,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":7717061,"work_id":17119386,"tagging_user_id":36693995,"tagged_user_id":235218309,"co_author_invite_id":1723467,"email":"l***l@howard.edu","display_order":0,"name":"Lalita Kaul","title":"Immunopathology of Marek's disease in quails: presence of antinuclear antibody and immune complex"}],"downloadable_attachments":[],"slug":"Immunopathology_of_Mareks_disease_in_quails_presence_of_antinuclear_antibody_and_immune_complex","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":36693995,"first_name":"Hare","middle_initials":"","last_name":"Pradhan","page_name":"HarePradhan","domain_name":"independent","created_at":"2015-10-21T11:07:05.415-07:00","display_name":"Hare Pradhan","url":"https://independent.academia.edu/HarePradhan"},"attachments":[],"research_interests":[{"id":173,"name":"Zoology","url":"https://www.academia.edu/Documents/in/Zoology"},{"id":132052,"name":"Marek Disease","url":"https://www.academia.edu/Documents/in/Marek_Disease"},{"id":323597,"name":"Fluorescent Antibody Technique","url":"https://www.academia.edu/Documents/in/Fluorescent_Antibody_Technique"},{"id":644860,"name":"Veterinary Sciences","url":"https://www.academia.edu/Documents/in/Veterinary_Sciences"},{"id":924535,"name":"Quail","url":"https://www.academia.edu/Documents/in/Quail"},{"id":1482586,"name":"Autoantibodies","url":"https://www.academia.edu/Documents/in/Autoantibodies"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="17119385"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/17119385/Antibody_directed_against_Mareks_disease_associated_tumor_surface_antigen_can_be_eluted_from_Mareks_disease_tumor_cells"><img alt="Research paper thumbnail of Antibody directed against Marek's disease-associated tumor surface antigen can be eluted from Marek's disease tumor cells" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/17119385/Antibody_directed_against_Mareks_disease_associated_tumor_surface_antigen_can_be_eluted_from_Mareks_disease_tumor_cells">Antibody directed against Marek's disease-associated tumor surface antigen can be eluted from Marek's disease tumor cells</a></div><div class="wp-workCard_item"><span>Veterinary Immunology and Immunopathology</span><span>, 1991</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Antibody directed against Marek&amp;#39;s disease-associated tumor surface antigen (MATSA) was el...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Antibody directed against Marek&amp;#39;s disease-associated tumor surface antigen (MATSA) was eluted from tumor cells of lymphomas and peripheral blood lymphocytes that were isolated from Marek&amp;#39;s disease virus-infected chickens. Feather follicular Marek&amp;#39;s disease virus (MDV) antigen could not be demonstrated with this antibody by indirect immunofluorescent (IF) staining. Monoclonal antibody directed against MATSA could completely block the activity of eluted antibody and vice versa. By indirect IF staining using eluted antibody and fluorescein isothiocyanate (FITC) labelled antichicken globulin conjugate. MATSA-bearing cells were detected in MDV infected and herpes virus of turkey (HVT) vaccinated birds. Blocking of immunoglobulin molecules present on B-cells by anti-chicken globulin is critical in this test.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="17119385"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="17119385"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 17119385; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=17119385]").text(description); $(".js-view-count[data-work-id=17119385]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 17119385; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='17119385']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 17119385, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=17119385]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":17119385,"title":"Antibody directed against Marek's disease-associated tumor surface antigen can be eluted from Marek's disease tumor cells","translated_title":"","metadata":{"abstract":"Antibody directed against Marek\u0026amp;#39;s disease-associated tumor surface antigen (MATSA) was eluted from tumor cells of lymphomas and peripheral blood lymphocytes that were isolated from Marek\u0026amp;#39;s disease virus-infected chickens. Feather follicular Marek\u0026amp;#39;s disease virus (MDV) antigen could not be demonstrated with this antibody by indirect immunofluorescent (IF) staining. Monoclonal antibody directed against MATSA could completely block the activity of eluted antibody and vice versa. By indirect IF staining using eluted antibody and fluorescein isothiocyanate (FITC) labelled antichicken globulin conjugate. MATSA-bearing cells were detected in MDV infected and herpes virus of turkey (HVT) vaccinated birds. Blocking of immunoglobulin molecules present on B-cells by anti-chicken globulin is critical in this test.","publication_date":{"day":null,"month":null,"year":1991,"errors":{}},"publication_name":"Veterinary Immunology and Immunopathology"},"translated_abstract":"Antibody directed against Marek\u0026amp;#39;s disease-associated tumor surface antigen (MATSA) was eluted from tumor cells of lymphomas and peripheral blood lymphocytes that were isolated from Marek\u0026amp;#39;s disease virus-infected chickens. Feather follicular Marek\u0026amp;#39;s disease virus (MDV) antigen could not be demonstrated with this antibody by indirect immunofluorescent (IF) staining. Monoclonal antibody directed against MATSA could completely block the activity of eluted antibody and vice versa. By indirect IF staining using eluted antibody and fluorescein isothiocyanate (FITC) labelled antichicken globulin conjugate. MATSA-bearing cells were detected in MDV infected and herpes virus of turkey (HVT) vaccinated birds. Blocking of immunoglobulin molecules present on B-cells by anti-chicken globulin is critical in this test.","internal_url":"https://www.academia.edu/17119385/Antibody_directed_against_Mareks_disease_associated_tumor_surface_antigen_can_be_eluted_from_Mareks_disease_tumor_cells","translated_internal_url":"","created_at":"2015-10-21T11:12:54.831-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":36693995,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":7717028,"work_id":17119385,"tagging_user_id":36693995,"tagged_user_id":16665419,"co_author_invite_id":null,"email":"b***t@gmail.com","display_order":0,"name":"B.Sushant Patnaik","title":"Antibody directed against Marek's disease-associated tumor surface antigen can be eluted from Marek's disease tumor cells"},{"id":7717086,"work_id":17119385,"tagging_user_id":36693995,"tagged_user_id":12271862,"co_author_invite_id":null,"email":"g***y@gmail.com","display_order":4194304,"name":"Gagan Mohanty","title":"Antibody directed against Marek's disease-associated tumor surface antigen can be eluted from Marek's disease tumor cells"},{"id":7717089,"work_id":17119385,"tagging_user_id":36693995,"tagged_user_id":58805523,"co_author_invite_id":1098759,"email":"w***e@ufl.edu","display_order":6291456,"name":"Won Suk Lee","title":"Antibody directed against Marek's disease-associated tumor surface antigen can be eluted from Marek's disease tumor cells"}],"downloadable_attachments":[],"slug":"Antibody_directed_against_Mareks_disease_associated_tumor_surface_antigen_can_be_eluted_from_Mareks_disease_tumor_cells","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":36693995,"first_name":"Hare","middle_initials":"","last_name":"Pradhan","page_name":"HarePradhan","domain_name":"independent","created_at":"2015-10-21T11:07:05.415-07:00","display_name":"Hare Pradhan","url":"https://independent.academia.edu/HarePradhan"},"attachments":[],"research_interests":[{"id":173,"name":"Zoology","url":"https://www.academia.edu/Documents/in/Zoology"},{"id":55269,"name":"Lymphoma","url":"https://www.academia.edu/Documents/in/Lymphoma"},{"id":132052,"name":"Marek Disease","url":"https://www.academia.edu/Documents/in/Marek_Disease"},{"id":147196,"name":"Monoclonal Antibodies","url":"https://www.academia.edu/Documents/in/Monoclonal_Antibodies"},{"id":323597,"name":"Fluorescent Antibody Technique","url":"https://www.academia.edu/Documents/in/Fluorescent_Antibody_Technique"},{"id":402759,"name":"Chickens","url":"https://www.academia.edu/Documents/in/Chickens"},{"id":644860,"name":"Veterinary Sciences","url":"https://www.academia.edu/Documents/in/Veterinary_Sciences"},{"id":782120,"name":"Veterinary Immunology","url":"https://www.academia.edu/Documents/in/Veterinary_Immunology"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="17119384"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/17119384/Immune_Complex_Mediated_Glomerulopathy_in_Mareks_Disease"><img alt="Research paper thumbnail of Immune Complex-Mediated Glomerulopathy in Marek's Disease" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/17119384/Immune_Complex_Mediated_Glomerulopathy_in_Mareks_Disease">Immune Complex-Mediated Glomerulopathy in Marek's Disease</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/HarePradhan">Hare Pradhan</a> and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/KatariaJ">J. Kataria</a></span></div><div class="wp-workCard_item"><span>Veterinary Immunology and Immunopathology</span><span>, 1988</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Chickens infected with Marek&amp;#39;s disease (MD) virus developed immune complex (IC)-mediated ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Chickens infected with Marek&amp;#39;s disease (MD) virus developed immune complex (IC)-mediated glomerulopathy. Fluorescent antibody staining technique using antichicken globulin and antichicken complement was used to demonstrate IC in the kidney glomeruli. During the initial stages of MDV infection, IC deposits were seen on the glomerular basement membrane, but subsequently the entire glomerulus was involved. Mesangial cells also had IC deposits. Chicken complement was demonstrated in the glomeruli which had IC deposits. The number of glomeruli with IC deposition was higher in tumor-bearing birds than in non-tumor-bearing birds. Histologically, kidney lesion were characterized by thickening of basement membrane and proliferation of mesangial cells. It is suggested that IC-mediated glomerulopathy might be one of the major causes of death in MD.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="17119384"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="17119384"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 17119384; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=17119384]").text(description); $(".js-view-count[data-work-id=17119384]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 17119384; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='17119384']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 17119384, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=17119384]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":17119384,"title":"Immune Complex-Mediated Glomerulopathy in Marek's Disease","translated_title":"","metadata":{"abstract":"Chickens infected with Marek\u0026amp;#39;s disease (MD) virus developed immune complex (IC)-mediated glomerulopathy. Fluorescent antibody staining technique using antichicken globulin and antichicken complement was used to demonstrate IC in the kidney glomeruli. During the initial stages of MDV infection, IC deposits were seen on the glomerular basement membrane, but subsequently the entire glomerulus was involved. Mesangial cells also had IC deposits. Chicken complement was demonstrated in the glomeruli which had IC deposits. The number of glomeruli with IC deposition was higher in tumor-bearing birds than in non-tumor-bearing birds. Histologically, kidney lesion were characterized by thickening of basement membrane and proliferation of mesangial cells. It is suggested that IC-mediated glomerulopathy might be one of the major causes of death in MD.","publication_date":{"day":null,"month":null,"year":1988,"errors":{}},"publication_name":"Veterinary Immunology and Immunopathology"},"translated_abstract":"Chickens infected with Marek\u0026amp;#39;s disease (MD) virus developed immune complex (IC)-mediated glomerulopathy. Fluorescent antibody staining technique using antichicken globulin and antichicken complement was used to demonstrate IC in the kidney glomeruli. During the initial stages of MDV infection, IC deposits were seen on the glomerular basement membrane, but subsequently the entire glomerulus was involved. Mesangial cells also had IC deposits. Chicken complement was demonstrated in the glomeruli which had IC deposits. The number of glomeruli with IC deposition was higher in tumor-bearing birds than in non-tumor-bearing birds. Histologically, kidney lesion were characterized by thickening of basement membrane and proliferation of mesangial cells. It is suggested that IC-mediated glomerulopathy might be one of the major causes of death in MD.","internal_url":"https://www.academia.edu/17119384/Immune_Complex_Mediated_Glomerulopathy_in_Mareks_Disease","translated_internal_url":"","created_at":"2015-10-21T11:12:54.729-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":36693995,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":7717060,"work_id":17119384,"tagging_user_id":36693995,"tagged_user_id":235218309,"co_author_invite_id":1723467,"email":"l***l@howard.edu","display_order":0,"name":"Lalita Kaul","title":"Immune Complex-Mediated Glomerulopathy in Marek's Disease"},{"id":7717085,"work_id":17119384,"tagging_user_id":36693995,"tagged_user_id":12271862,"co_author_invite_id":null,"email":"g***y@gmail.com","display_order":4194304,"name":"Gagan Mohanty","title":"Immune Complex-Mediated Glomerulopathy in Marek's Disease"},{"id":7717088,"work_id":17119384,"tagging_user_id":36693995,"tagged_user_id":58805523,"co_author_invite_id":1098759,"email":"w***e@ufl.edu","display_order":6291456,"name":"Won Suk Lee","title":"Immune Complex-Mediated Glomerulopathy in Marek's Disease"},{"id":7717090,"work_id":17119384,"tagging_user_id":36693995,"tagged_user_id":36804842,"co_author_invite_id":1723477,"email":"j***a@rediffmail.com","display_order":7340032,"name":"J. Kataria","title":"Immune Complex-Mediated Glomerulopathy in Marek's Disease"}],"downloadable_attachments":[],"slug":"Immune_Complex_Mediated_Glomerulopathy_in_Mareks_Disease","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":36693995,"first_name":"Hare","middle_initials":"","last_name":"Pradhan","page_name":"HarePradhan","domain_name":"independent","created_at":"2015-10-21T11:07:05.415-07:00","display_name":"Hare Pradhan","url":"https://independent.academia.edu/HarePradhan"},"attachments":[],"research_interests":[{"id":173,"name":"Zoology","url":"https://www.academia.edu/Documents/in/Zoology"},{"id":132052,"name":"Marek Disease","url":"https://www.academia.edu/Documents/in/Marek_Disease"},{"id":323597,"name":"Fluorescent Antibody Technique","url":"https://www.academia.edu/Documents/in/Fluorescent_Antibody_Technique"},{"id":366620,"name":"POULTRY DISEASES","url":"https://www.academia.edu/Documents/in/POULTRY_DISEASES"},{"id":402759,"name":"Chickens","url":"https://www.academia.edu/Documents/in/Chickens"},{"id":644860,"name":"Veterinary Sciences","url":"https://www.academia.edu/Documents/in/Veterinary_Sciences"},{"id":782120,"name":"Veterinary Immunology","url":"https://www.academia.edu/Documents/in/Veterinary_Immunology"},{"id":1601249,"name":"Glomerulonephritis","url":"https://www.academia.edu/Documents/in/Glomerulonephritis"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="17119383"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/17119383/The_safety_and_efficacy_of_the_oral_rabies_vaccine_SAG2_in_Indian_stray_dogs"><img alt="Research paper thumbnail of The safety and efficacy of the oral rabies vaccine SAG2 in Indian stray dogs" class="work-thumbnail" src="https://attachments.academia-assets.com/42316768/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/17119383/The_safety_and_efficacy_of_the_oral_rabies_vaccine_SAG2_in_Indian_stray_dogs">The safety and efficacy of the oral rabies vaccine SAG2 in Indian stray dogs</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/HarePradhan">Hare Pradhan</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/FlorenceCliquet">Florence Cliquet</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/JGurbuxani">J. Gurbuxani</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/RaghvendarSingh">Raghvendar Singh</a>, and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/CharlesRupprecht">Charles Rupprecht</a></span></div><div class="wp-workCard_item"><span>Vaccine</span><span>, 2007</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="cacb55fc4bf397f0617d455f9bc5dfe2" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":42316768,"asset_id":17119383,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/42316768/download_file?st=MTczMzE5NzgyMSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="17119383"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="17119383"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 17119383; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=17119383]").text(description); $(".js-view-count[data-work-id=17119383]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 17119383; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='17119383']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 17119383, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "cacb55fc4bf397f0617d455f9bc5dfe2" } } $('.js-work-strip[data-work-id=17119383]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":17119383,"title":"The safety and efficacy of the oral rabies vaccine SAG2 in Indian stray dogs","translated_title":"","metadata":{"ai_title_tag":"Safety and Efficacy of Oral Rabies Vaccine SAG2 in Stray Dogs","grobid_abstract":"India is one of the countries with the highest prevalence of human rabies throughout the world. Dogs are primarily responsible for rabies transmission. Among them, stray dogs play a major role in that country. Parenteral vaccination programmes are insufficient to eliminate rabies partly due to difficulties in establishing satisfactory immunisation coverage in the dog population in view of the high proportion of stray dogs. Oral vaccination may be a useful adjunct to parenteral vaccination by increasing dog vaccination coverage. Safety, immunogenicity and efficacy of Rabidog ® SAG2 bait were evaluated in Indian stray dogs in captivity.","publication_date":{"day":null,"month":null,"year":2007,"errors":{}},"publication_name":"Vaccine","grobid_abstract_attachment_id":42316768},"translated_abstract":null,"internal_url":"https://www.academia.edu/17119383/The_safety_and_efficacy_of_the_oral_rabies_vaccine_SAG2_in_Indian_stray_dogs","translated_internal_url":"","created_at":"2015-10-21T11:12:54.627-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":36693995,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":7717019,"work_id":17119383,"tagging_user_id":36693995,"tagged_user_id":null,"co_author_invite_id":124581,"email":"p***b@gmail.com","display_order":0,"name":"B. Pattnaik","title":"The safety and efficacy of the oral rabies vaccine SAG2 in Indian stray dogs"},{"id":7717025,"work_id":17119383,"tagging_user_id":36693995,"tagged_user_id":7223595,"co_author_invite_id":null,"email":"d***l@gmail.com","display_order":4194304,"name":"Suresh Patil","title":"The safety and efficacy of the oral rabies vaccine SAG2 in Indian stray dogs"},{"id":7717052,"work_id":17119383,"tagging_user_id":36693995,"tagged_user_id":29844908,"co_author_invite_id":null,"email":"r***d@icar.gov.in","display_order":6291456,"name":"Richa Sood","title":"The safety and efficacy of the oral rabies vaccine SAG2 in Indian stray dogs"},{"id":7717071,"work_id":17119383,"tagging_user_id":36693995,"tagged_user_id":36906653,"co_author_invite_id":45757,"email":"f***t@anses.fr","display_order":7340032,"name":"Florence Cliquet","title":"The safety and efficacy of the oral rabies vaccine SAG2 in Indian stray dogs"},{"id":7717072,"work_id":17119383,"tagging_user_id":36693995,"tagged_user_id":36845280,"co_author_invite_id":1723472,"email":"j***x@gmail.com","display_order":7864320,"name":"J. Gurbuxani","title":"The safety and efficacy of the oral rabies vaccine SAG2 in Indian stray dogs"},{"id":7717073,"work_id":17119383,"tagging_user_id":36693995,"tagged_user_id":35746427,"co_author_invite_id":null,"email":"a***t@inserm.fr","display_order":8126464,"name":"Armelle Regnault","title":"The safety and efficacy of the oral rabies vaccine SAG2 in Indian stray dogs"},{"id":7717074,"work_id":17119383,"tagging_user_id":36693995,"tagged_user_id":null,"co_author_invite_id":1723473,"email":"a***t@bpg.it","display_order":8257536,"name":"A. Guiot","title":"The safety and efficacy of the oral rabies vaccine SAG2 in Indian stray dogs"},{"id":7717075,"work_id":17119383,"tagging_user_id":36693995,"tagged_user_id":null,"co_author_invite_id":1723474,"email":"p***l@virbac.fr","display_order":8323072,"name":"P. Mahl","title":"The safety and efficacy of the oral rabies vaccine SAG2 in Indian stray dogs"},{"id":7717076,"work_id":17119383,"tagging_user_id":36693995,"tagged_user_id":33107915,"co_author_invite_id":null,"email":"r***r@gmail.com","display_order":8355840,"name":"Raghvendar Singh","title":"The safety and efficacy of the oral rabies vaccine SAG2 in Indian stray dogs"},{"id":7717077,"work_id":17119383,"tagging_user_id":36693995,"tagged_user_id":null,"co_author_invite_id":1259030,"email":"m***f@who.int","display_order":8372224,"name":"F. Meslin","title":"The safety and efficacy of the oral rabies vaccine SAG2 in Indian stray dogs"},{"id":7717078,"work_id":17119383,"tagging_user_id":36693995,"tagged_user_id":null,"co_author_invite_id":1723475,"email":"e***p@gmail.com","display_order":8380416,"name":"E. Picard","title":"The safety and efficacy of the oral rabies vaccine SAG2 in Indian stray dogs"},{"id":7717079,"work_id":17119383,"tagging_user_id":36693995,"tagged_user_id":null,"co_author_invite_id":1723476,"email":"m***t@iiwelding.org","display_order":8384512,"name":"M. Aubert","title":"The safety and efficacy of the oral rabies vaccine SAG2 in Indian stray dogs"},{"id":7717080,"work_id":17119383,"tagging_user_id":36693995,"tagged_user_id":null,"co_author_invite_id":1624284,"email":"j***t@ifa-fiv.org","display_order":8386560,"name":"J. Barrat","title":"The safety and efficacy of the oral rabies vaccine SAG2 in Indian stray dogs"},{"id":7717081,"work_id":17119383,"tagging_user_id":36693995,"tagged_user_id":24656179,"co_author_invite_id":null,"email":"m***r@gmail.com","display_order":8387584,"name":"Muu Nir","title":"The safety and efficacy of the oral rabies vaccine SAG2 in Indian stray dogs"},{"id":7717082,"work_id":17119383,"tagging_user_id":36693995,"tagged_user_id":36153531,"co_author_invite_id":null,"email":"j***y@envt.fr","display_order":8388096,"name":"Jean-denis Bailly","title":"The safety and efficacy of the oral rabies vaccine SAG2 in Indian stray dogs"},{"id":7717083,"work_id":17119383,"tagging_user_id":36693995,"tagged_user_id":36820555,"co_author_invite_id":387346,"email":"c***t@yahoo.com","display_order":8388352,"name":"Charles Rupprecht","title":"The safety and efficacy of the oral rabies vaccine SAG2 in Indian stray dogs"}],"downloadable_attachments":[{"id":42316768,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/42316768/thumbnails/1.jpg","file_name":"The_safety_and_efficacy_of_the_oral_rabi20160207-27897-lnztl7.pdf","download_url":"https://www.academia.edu/attachments/42316768/download_file?st=MTczMzE5NzgyMSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"The_safety_and_efficacy_of_the_oral_rabi.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/42316768/The_safety_and_efficacy_of_the_oral_rabi20160207-27897-lnztl7-libre.pdf?1454868700=\u0026response-content-disposition=attachment%3B+filename%3DThe_safety_and_efficacy_of_the_oral_rabi.pdf\u0026Expires=1733201421\u0026Signature=dA7iIN9Nw5uJMFOfgmKLsmx4r8LbHG7UEkY0St~4ht3nYuOISp6qWKoJET19c53oSmploPrBpF1y1La4iM9VCN6aYgPosNOkMQkuJtuzSIfiOSwvwJEtEnJJY9jsvHlWnFJrHUMRfL5qbNfTZjrbwup6Jgd1BKllQG6Fi60mtqF820FteKB8OzwcTWmU6WI834a6kLo5sA~hEi~4WfLs4pyKIBgoA~pP4Hgl3FWN2ARewizpKsHB0qgkJBrR5RwkNMQjubScUa6hHIHfduh4qTO3O0oFrwHH~1FFlZSP21TAopugBYi4t~0rbXYvlV3EWDkoZAMJ6owBeEIWfHJbPA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"The_safety_and_efficacy_of_the_oral_rabies_vaccine_SAG2_in_Indian_stray_dogs","translated_slug":"","page_count":10,"language":"en","content_type":"Work","owner":{"id":36693995,"first_name":"Hare","middle_initials":"","last_name":"Pradhan","page_name":"HarePradhan","domain_name":"independent","created_at":"2015-10-21T11:07:05.415-07:00","display_name":"Hare Pradhan","url":"https://independent.academia.edu/HarePradhan"},"attachments":[{"id":42316768,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/42316768/thumbnails/1.jpg","file_name":"The_safety_and_efficacy_of_the_oral_rabi20160207-27897-lnztl7.pdf","download_url":"https://www.academia.edu/attachments/42316768/download_file?st=MTczMzE5NzgyMSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"The_safety_and_efficacy_of_the_oral_rabi.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/42316768/The_safety_and_efficacy_of_the_oral_rabi20160207-27897-lnztl7-libre.pdf?1454868700=\u0026response-content-disposition=attachment%3B+filename%3DThe_safety_and_efficacy_of_the_oral_rabi.pdf\u0026Expires=1733201421\u0026Signature=dA7iIN9Nw5uJMFOfgmKLsmx4r8LbHG7UEkY0St~4ht3nYuOISp6qWKoJET19c53oSmploPrBpF1y1La4iM9VCN6aYgPosNOkMQkuJtuzSIfiOSwvwJEtEnJJY9jsvHlWnFJrHUMRfL5qbNfTZjrbwup6Jgd1BKllQG6Fi60mtqF820FteKB8OzwcTWmU6WI834a6kLo5sA~hEi~4WfLs4pyKIBgoA~pP4Hgl3FWN2ARewizpKsHB0qgkJBrR5RwkNMQjubScUa6hHIHfduh4qTO3O0oFrwHH~1FFlZSP21TAopugBYi4t~0rbXYvlV3EWDkoZAMJ6owBeEIWfHJbPA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":1199,"name":"Eastern Europe","url":"https://www.academia.edu/Documents/in/Eastern_Europe"},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences"},{"id":49161,"name":"Safety","url":"https://www.academia.edu/Documents/in/Safety"},{"id":52438,"name":"Dogs","url":"https://www.academia.edu/Documents/in/Dogs"},{"id":150847,"name":"Vaccine","url":"https://www.academia.edu/Documents/in/Vaccine"},{"id":179934,"name":"Vaccination","url":"https://www.academia.edu/Documents/in/Vaccination"},{"id":289330,"name":"Prevalence","url":"https://www.academia.edu/Documents/in/Prevalence"},{"id":344106,"name":"Saliva","url":"https://www.academia.edu/Documents/in/Saliva"},{"id":483642,"name":"Rabies Vaccines","url":"https://www.academia.edu/Documents/in/Rabies_Vaccines"},{"id":966167,"name":"Salivary Gland","url":"https://www.academia.edu/Documents/in/Salivary_Gland"},{"id":988725,"name":"Clinical Signs","url":"https://www.academia.edu/Documents/in/Clinical_Signs"},{"id":1010136,"name":"Rabies Virus","url":"https://www.academia.edu/Documents/in/Rabies_Virus"},{"id":1703190,"name":"Neutralizing antibodies","url":"https://www.academia.edu/Documents/in/Neutralizing_antibodies"},{"id":1944473,"name":"Oral Vaccination","url":"https://www.academia.edu/Documents/in/Oral_Vaccination"}],"urls":[{"id":6522415,"url":"https://www.researchgate.net/profile/Richa_Sood2/publication/6578468_The_safety_and_efficacy_of_the_oral_rabies_vaccine_SAG2_in_Indian_stray_dogs/links/542693f20cf26120b7b0b065.pdf"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="17119382"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/17119382/Outbreaks_of_egg_drop_syndrome_due_to_EDS_76_virus_in_quail_Coturnix_coturnix_japonica_"><img alt="Research paper thumbnail of Outbreaks of egg drop syndrome due to EDS-76 virus in quail (Coturnix coturnix japonica)" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/17119382/Outbreaks_of_egg_drop_syndrome_due_to_EDS_76_virus_in_quail_Coturnix_coturnix_japonica_">Outbreaks of egg drop syndrome due to EDS-76 virus in quail (Coturnix coturnix japonica)</a></div><div class="wp-workCard_item"><span>Veterinary Record</span><span>, 1992</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Two outbreaks of the egg drop syndrome were observed in quail flocks maintained on a farm togethe...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Two outbreaks of the egg drop syndrome were observed in quail flocks maintained on a farm together with chickens. The decrease in egg production ranged from 10.6 per cent to 50.6 per cent, and the number of soft-shelled eggs increased with the decline in egg production. In both the outbreaks haemagglutination inhibiting antibodies to EDS-76 virus were detected. Fluorescent viral antigen specific to EDS-76 virus was also detected in the lining epithelial and glandular cells of the uterus.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="17119382"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="17119382"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 17119382; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=17119382]").text(description); $(".js-view-count[data-work-id=17119382]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 17119382; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='17119382']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 17119382, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=17119382]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":17119382,"title":"Outbreaks of egg drop syndrome due to EDS-76 virus in quail (Coturnix coturnix japonica)","translated_title":"","metadata":{"abstract":"Two outbreaks of the egg drop syndrome were observed in quail flocks maintained on a farm together with chickens. The decrease in egg production ranged from 10.6 per cent to 50.6 per cent, and the number of soft-shelled eggs increased with the decline in egg production. In both the outbreaks haemagglutination inhibiting antibodies to EDS-76 virus were detected. Fluorescent viral antigen specific to EDS-76 virus was also detected in the lining epithelial and glandular cells of the uterus.","publication_date":{"day":null,"month":null,"year":1992,"errors":{}},"publication_name":"Veterinary Record"},"translated_abstract":"Two outbreaks of the egg drop syndrome were observed in quail flocks maintained on a farm together with chickens. The decrease in egg production ranged from 10.6 per cent to 50.6 per cent, and the number of soft-shelled eggs increased with the decline in egg production. In both the outbreaks haemagglutination inhibiting antibodies to EDS-76 virus were detected. Fluorescent viral antigen specific to EDS-76 virus was also detected in the lining epithelial and glandular cells of the uterus.","internal_url":"https://www.academia.edu/17119382/Outbreaks_of_egg_drop_syndrome_due_to_EDS_76_virus_in_quail_Coturnix_coturnix_japonica_","translated_internal_url":"","created_at":"2015-10-21T11:12:54.536-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":36693995,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":7717070,"work_id":17119382,"tagging_user_id":36693995,"tagged_user_id":28628339,"co_author_invite_id":null,"email":"b***y@gmail.com","affiliation":"West Bengal University of Animal and Fishery Sciences","display_order":0,"name":"Bipul Kumar Das","title":"Outbreaks of egg drop syndrome due to EDS-76 virus in quail (Coturnix coturnix japonica)"}],"downloadable_attachments":[],"slug":"Outbreaks_of_egg_drop_syndrome_due_to_EDS_76_virus_in_quail_Coturnix_coturnix_japonica_","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":36693995,"first_name":"Hare","middle_initials":"","last_name":"Pradhan","page_name":"HarePradhan","domain_name":"independent","created_at":"2015-10-21T11:07:05.415-07:00","display_name":"Hare Pradhan","url":"https://independent.academia.edu/HarePradhan"},"attachments":[],"research_interests":[{"id":43838,"name":"India","url":"https://www.academia.edu/Documents/in/India"},{"id":57907,"name":"Disease Outbreaks","url":"https://www.academia.edu/Documents/in/Disease_Outbreaks"},{"id":366620,"name":"POULTRY DISEASES","url":"https://www.academia.edu/Documents/in/POULTRY_DISEASES"},{"id":499699,"name":"Oviposition","url":"https://www.academia.edu/Documents/in/Oviposition"},{"id":644860,"name":"Veterinary Sciences","url":"https://www.academia.edu/Documents/in/Veterinary_Sciences"},{"id":1225171,"name":"Syndrome","url":"https://www.academia.edu/Documents/in/Syndrome"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="17119381"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/17119381/Identification_of_bovine_viral_diarrhea_virus_type_1_in_yaks_Bos_poephagus_grunniens_in_the_Himalayan_region"><img alt="Research paper thumbnail of Identification of bovine viral diarrhea virus type 1 in yaks (Bos poephagus grunniens) in the Himalayan region" class="work-thumbnail" src="https://attachments.academia-assets.com/42316754/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/17119381/Identification_of_bovine_viral_diarrhea_virus_type_1_in_yaks_Bos_poephagus_grunniens_in_the_Himalayan_region">Identification of bovine viral diarrhea virus type 1 in yaks (Bos poephagus grunniens) in the Himalayan region</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/HarePradhan">Hare Pradhan</a> and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/ATiwari1">A. Tiwari</a></span></div><div class="wp-workCard_item"><span>Research in Veterinary Science</span><span>, 2008</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="3899d6437447c9fcd3bf11e3c59ce8db" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":42316754,"asset_id":17119381,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/42316754/download_file?st=MTczMzE5NzgyMSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="17119381"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="17119381"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 17119381; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=17119381]").text(description); $(".js-view-count[data-work-id=17119381]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 17119381; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='17119381']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 17119381, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "3899d6437447c9fcd3bf11e3c59ce8db" } } $('.js-work-strip[data-work-id=17119381]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":17119381,"title":"Identification of bovine viral diarrhea virus type 1 in yaks (Bos poephagus grunniens) in the Himalayan region","translated_title":"","metadata":{"grobid_abstract":"Since cattle are widely infected by bovine viral diarrhea virus (BVDV) in India, we searched for pestivirus infection in yaks. Of 71 pure and crossbred yaks from Himalayan region, pestivirus antigen was detected by Ag-ELISA in three animals. Pestivirus in leukocyte and cell culture isolated virus samples originating from positive yaks was also confirmed by RT-PCR using panpestivirus specific primers selected from 5 0 -untranslated region (5 0 UTR). The 5 0 UTR, N pro and E2 regions were sequenced and used for genetic typing. Phylogenetic analysis revealed that pestiviruses detected in three Himalayan yaks were similar genetically, belonging to BVDV-1. Antigenic characterisation of yak pestivirus also confirmed the typing as BVDV-1. This is the first report on the identification of BVDV type 1 in yaks.","publication_date":{"day":null,"month":null,"year":2008,"errors":{}},"publication_name":"Research in Veterinary Science","grobid_abstract_attachment_id":42316754},"translated_abstract":null,"internal_url":"https://www.academia.edu/17119381/Identification_of_bovine_viral_diarrhea_virus_type_1_in_yaks_Bos_poephagus_grunniens_in_the_Himalayan_region","translated_internal_url":"","created_at":"2015-10-21T11:12:54.428-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":36693995,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":7717032,"work_id":17119381,"tagging_user_id":36693995,"tagged_user_id":50823026,"co_author_invite_id":616470,"email":"m***r@rediffmail.com","display_order":0,"name":"Niranjan Mishra","title":"Identification of bovine viral diarrhea virus type 1 in yaks (Bos poephagus grunniens) in the Himalayan region"},{"id":7717062,"work_id":17119381,"tagging_user_id":36693995,"tagged_user_id":null,"co_author_invite_id":1723468,"email":"k***r@rediffmail.com","display_order":4194304,"name":"K. Rajukumar","title":"Identification of bovine viral diarrhea virus type 1 in yaks (Bos poephagus grunniens) in the Himalayan region"},{"id":7717065,"work_id":17119381,"tagging_user_id":36693995,"tagged_user_id":null,"co_author_invite_id":1723470,"email":"r***u@rediffmail.com","display_order":6291456,"name":"R. Dubey","title":"Identification of bovine viral diarrhea virus type 1 in yaks (Bos poephagus grunniens) in the Himalayan region"},{"id":7717067,"work_id":17119381,"tagging_user_id":36693995,"tagged_user_id":36958264,"co_author_invite_id":1723471,"email":"a***3@yahoo.com","display_order":7340032,"name":"A. Tiwari","title":"Identification of bovine viral diarrhea virus type 1 in yaks (Bos poephagus grunniens) in the Himalayan region"},{"id":7717069,"work_id":17119381,"tagging_user_id":36693995,"tagged_user_id":10236221,"co_author_invite_id":null,"email":"v***v@rediffmail.com","display_order":7864320,"name":"Alka Galav","title":"Identification of bovine viral diarrhea virus type 1 in yaks (Bos poephagus grunniens) in the Himalayan region"},{"id":7717098,"work_id":17119381,"tagging_user_id":36693995,"tagged_user_id":33035758,"co_author_invite_id":null,"email":"v***k@uvm.sk","display_order":8126464,"name":"Stefan Vilcek","title":"Identification of bovine viral diarrhea virus type 1 in yaks (Bos poephagus grunniens) in the Himalayan region"}],"downloadable_attachments":[{"id":42316754,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/42316754/thumbnails/1.jpg","file_name":"00b7d5281ff459e88f000000.pdf","download_url":"https://www.academia.edu/attachments/42316754/download_file?st=MTczMzE5NzgyMSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Identification_of_bovine_viral_diarrhea.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/42316754/00b7d5281ff459e88f000000-libre.pdf?1454868701=\u0026response-content-disposition=attachment%3B+filename%3DIdentification_of_bovine_viral_diarrhea.pdf\u0026Expires=1733201421\u0026Signature=EohykNIbxuLCewRZjlZMyJL1h0tkYBV6px~G~B4DmezBXX2hnMjz7u3byHWJ0YNpIWtyPseth8dNc3NNpFG6ra-8M0xEESILGfauWVEYY6faNumcOLKfSHryP2dbgyPxiHJWjPzsA5qEZdkapY0HhYsktDuKNycd67G4nz~18boxB4pAppAebF6-mp~ne3uazRKnKEPF0BM0CdQa-5c8u6vGhu7eVdZ1~KCYfMJzJ3oSEwpI0lZyJNIzXv8yS0grfAgOyiEwS4Y8I7UmfXHHe7DvAlwUXwlewBBHEqLzT1op8KziODS2~8FMQaZL~6lX~oxCF99~DV-Bzhxqvm2BJw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Identification_of_bovine_viral_diarrhea_virus_type_1_in_yaks_Bos_poephagus_grunniens_in_the_Himalayan_region","translated_slug":"","page_count":4,"language":"en","content_type":"Work","owner":{"id":36693995,"first_name":"Hare","middle_initials":"","last_name":"Pradhan","page_name":"HarePradhan","domain_name":"independent","created_at":"2015-10-21T11:07:05.415-07:00","display_name":"Hare Pradhan","url":"https://independent.academia.edu/HarePradhan"},"attachments":[{"id":42316754,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/42316754/thumbnails/1.jpg","file_name":"00b7d5281ff459e88f000000.pdf","download_url":"https://www.academia.edu/attachments/42316754/download_file?st=MTczMzE5NzgyMSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Identification_of_bovine_viral_diarrhea.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/42316754/00b7d5281ff459e88f000000-libre.pdf?1454868701=\u0026response-content-disposition=attachment%3B+filename%3DIdentification_of_bovine_viral_diarrhea.pdf\u0026Expires=1733201421\u0026Signature=EohykNIbxuLCewRZjlZMyJL1h0tkYBV6px~G~B4DmezBXX2hnMjz7u3byHWJ0YNpIWtyPseth8dNc3NNpFG6ra-8M0xEESILGfauWVEYY6faNumcOLKfSHryP2dbgyPxiHJWjPzsA5qEZdkapY0HhYsktDuKNycd67G4nz~18boxB4pAppAebF6-mp~ne3uazRKnKEPF0BM0CdQa-5c8u6vGhu7eVdZ1~KCYfMJzJ3oSEwpI0lZyJNIzXv8yS0grfAgOyiEwS4Y8I7UmfXHHe7DvAlwUXwlewBBHEqLzT1op8KziODS2~8FMQaZL~6lX~oxCF99~DV-Bzhxqvm2BJw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":156,"name":"Genetics","url":"https://www.academia.edu/Documents/in/Genetics"},{"id":6697,"name":"Australia","url":"https://www.academia.edu/Documents/in/Australia"},{"id":29980,"name":"Animal Production","url":"https://www.academia.edu/Documents/in/Animal_Production"},{"id":35414,"name":"Veterinary","url":"https://www.academia.edu/Documents/in/Veterinary"},{"id":43294,"name":"Veterinary Science","url":"https://www.academia.edu/Documents/in/Veterinary_Science"},{"id":43838,"name":"India","url":"https://www.academia.edu/Documents/in/India"},{"id":50576,"name":"Germany","url":"https://www.academia.edu/Documents/in/Germany"},{"id":54433,"name":"Phylogeny","url":"https://www.academia.edu/Documents/in/Phylogeny"},{"id":158782,"name":"Phylogenetic analysis","url":"https://www.academia.edu/Documents/in/Phylogenetic_analysis"},{"id":260829,"name":"Cattle","url":"https://www.academia.edu/Documents/in/Cattle"},{"id":644860,"name":"Veterinary Sciences","url":"https://www.academia.edu/Documents/in/Veterinary_Sciences"},{"id":1709309,"name":"Bovine Viral Diarrhea Virus","url":"https://www.academia.edu/Documents/in/Bovine_Viral_Diarrhea_Virus"},{"id":2056389,"name":"Pestivirus","url":"https://www.academia.edu/Documents/in/Pestivirus"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="17119380"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/17119380/Development_and_evaluation_of_a_MAb_based_competitive_ELISA_using_helicase_domain_of_NS3_protein_for_sero_diagnosis_of_bovine_viral_diarrhea_in_cattle_and_buffaloes"><img alt="Research paper thumbnail of Development and evaluation of a MAb based competitive-ELISA using helicase domain of NS3 protein for sero-diagnosis of bovine viral diarrhea in cattle and buffaloes" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/17119380/Development_and_evaluation_of_a_MAb_based_competitive_ELISA_using_helicase_domain_of_NS3_protein_for_sero_diagnosis_of_bovine_viral_diarrhea_in_cattle_and_buffaloes">Development and evaluation of a MAb based competitive-ELISA using helicase domain of NS3 protein for sero-diagnosis of bovine viral diarrhea in cattle and buffaloes</a></div><div class="wp-workCard_item"><span>Research in Veterinary Science</span><span>, 2008</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The aim of this study was to develop a competitive inhibition ELISA (CI-ELISA) for detection of a...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The aim of this study was to develop a competitive inhibition ELISA (CI-ELISA) for detection of antibodies to bovine viral diarrhea virus (BVDV) using the helicase domain of NS3 (non-structural) protein and monoclonal antibody (MAb) against it and to estimate its sensitivity and specificity using two commercial ELISA kits as independent references. The 45-kDa helicase domain of NS3 protein of BVDV was expressed in Escherichia coli and 18MAbs were developed against it. MAb-11G8 was selected for use in CI-ELISA on the basis of maximum inhibition (90%) obtained with BVDV type 1 infected calf serum. Based on the distribution of percent inhibition of known negative sera (n=166), a cut-off value was set at 40% inhibition. In testing 914 field serum samples of cattle (810) and buffaloes (104), the CI-ELISA showed a relative specificity of 95.75% and 97.38% and sensitivity of 96% and 94.43% with Ingenesa kit and Institut Pourquier kit, respectively. This study proved that the use of helicase domain of NS3 (45-kDa) is equally good as the whole NS3 protein (80-kDa) used in commercial kits for detection of BVDV antibodies in cattle and buffaloes.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="17119380"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="17119380"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 17119380; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=17119380]").text(description); $(".js-view-count[data-work-id=17119380]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 17119380; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='17119380']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 17119380, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=17119380]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":17119380,"title":"Development and evaluation of a MAb based competitive-ELISA using helicase domain of NS3 protein for sero-diagnosis of bovine viral diarrhea in cattle and buffaloes","translated_title":"","metadata":{"abstract":"The aim of this study was to develop a competitive inhibition ELISA (CI-ELISA) for detection of antibodies to bovine viral diarrhea virus (BVDV) using the helicase domain of NS3 (non-structural) protein and monoclonal antibody (MAb) against it and to estimate its sensitivity and specificity using two commercial ELISA kits as independent references. The 45-kDa helicase domain of NS3 protein of BVDV was expressed in Escherichia coli and 18MAbs were developed against it. MAb-11G8 was selected for use in CI-ELISA on the basis of maximum inhibition (90%) obtained with BVDV type 1 infected calf serum. Based on the distribution of percent inhibition of known negative sera (n=166), a cut-off value was set at 40% inhibition. In testing 914 field serum samples of cattle (810) and buffaloes (104), the CI-ELISA showed a relative specificity of 95.75% and 97.38% and sensitivity of 96% and 94.43% with Ingenesa kit and Institut Pourquier kit, respectively. This study proved that the use of helicase domain of NS3 (45-kDa) is equally good as the whole NS3 protein (80-kDa) used in commercial kits for detection of BVDV antibodies in cattle and buffaloes.","publication_date":{"day":null,"month":null,"year":2008,"errors":{}},"publication_name":"Research in Veterinary Science"},"translated_abstract":"The aim of this study was to develop a competitive inhibition ELISA (CI-ELISA) for detection of antibodies to bovine viral diarrhea virus (BVDV) using the helicase domain of NS3 (non-structural) protein and monoclonal antibody (MAb) against it and to estimate its sensitivity and specificity using two commercial ELISA kits as independent references. The 45-kDa helicase domain of NS3 protein of BVDV was expressed in Escherichia coli and 18MAbs were developed against it. MAb-11G8 was selected for use in CI-ELISA on the basis of maximum inhibition (90%) obtained with BVDV type 1 infected calf serum. Based on the distribution of percent inhibition of known negative sera (n=166), a cut-off value was set at 40% inhibition. In testing 914 field serum samples of cattle (810) and buffaloes (104), the CI-ELISA showed a relative specificity of 95.75% and 97.38% and sensitivity of 96% and 94.43% with Ingenesa kit and Institut Pourquier kit, respectively. This study proved that the use of helicase domain of NS3 (45-kDa) is equally good as the whole NS3 protein (80-kDa) used in commercial kits for detection of BVDV antibodies in cattle and buffaloes.","internal_url":"https://www.academia.edu/17119380/Development_and_evaluation_of_a_MAb_based_competitive_ELISA_using_helicase_domain_of_NS3_protein_for_sero_diagnosis_of_bovine_viral_diarrhea_in_cattle_and_buffaloes","translated_internal_url":"","created_at":"2015-10-21T11:12:54.320-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":36693995,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":7717023,"work_id":17119380,"tagging_user_id":36693995,"tagged_user_id":null,"co_author_invite_id":124581,"email":"p***b@gmail.com","display_order":0,"name":"B. Pattnaik","title":"Development and evaluation of a MAb based competitive-ELISA using helicase domain of NS3 protein for sero-diagnosis of bovine viral diarrhea in cattle and buffaloes"},{"id":7717035,"work_id":17119380,"tagging_user_id":36693995,"tagged_user_id":50823026,"co_author_invite_id":616470,"email":"m***r@rediffmail.com","display_order":4194304,"name":"Niranjan Mishra","title":"Development and evaluation of a MAb based competitive-ELISA using helicase domain of NS3 protein for sero-diagnosis of bovine viral diarrhea in cattle and buffaloes"},{"id":7717050,"work_id":17119380,"tagging_user_id":36693995,"tagged_user_id":null,"co_author_invite_id":1723465,"email":"b***n@yahoo.co.in","display_order":6291456,"name":"S. Bhatia","title":"Development and evaluation of a MAb based competitive-ELISA using helicase domain of NS3 protein for sero-diagnosis of bovine viral diarrhea in cattle and buffaloes"},{"id":7717054,"work_id":17119380,"tagging_user_id":36693995,"tagged_user_id":29844908,"co_author_invite_id":null,"email":"r***d@icar.gov.in","display_order":7340032,"name":"Richa Sood","title":"Development and evaluation of a MAb based competitive-ELISA using helicase domain of NS3 protein for sero-diagnosis of bovine viral diarrhea in cattle and buffaloes"}],"downloadable_attachments":[],"slug":"Development_and_evaluation_of_a_MAb_based_competitive_ELISA_using_helicase_domain_of_NS3_protein_for_sero_diagnosis_of_bovine_viral_diarrhea_in_cattle_and_buffaloes","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":36693995,"first_name":"Hare","middle_initials":"","last_name":"Pradhan","page_name":"HarePradhan","domain_name":"independent","created_at":"2015-10-21T11:07:05.415-07:00","display_name":"Hare Pradhan","url":"https://independent.academia.edu/HarePradhan"},"attachments":[],"research_interests":[{"id":29980,"name":"Animal Production","url":"https://www.academia.edu/Documents/in/Animal_Production"},{"id":35414,"name":"Veterinary","url":"https://www.academia.edu/Documents/in/Veterinary"},{"id":43294,"name":"Veterinary Science","url":"https://www.academia.edu/Documents/in/Veterinary_Science"},{"id":83128,"name":"Escherichia coli","url":"https://www.academia.edu/Documents/in/Escherichia_coli"},{"id":147196,"name":"Monoclonal Antibodies","url":"https://www.academia.edu/Documents/in/Monoclonal_Antibodies"},{"id":260829,"name":"Cattle","url":"https://www.academia.edu/Documents/in/Cattle"},{"id":644860,"name":"Veterinary Sciences","url":"https://www.academia.edu/Documents/in/Veterinary_Sciences"},{"id":766014,"name":"Monoclonal Antibody","url":"https://www.academia.edu/Documents/in/Monoclonal_Antibody"},{"id":901876,"name":"Sensitivity and Specificity","url":"https://www.academia.edu/Documents/in/Sensitivity_and_Specificity"},{"id":1272906,"name":"Enzyme Linked Immunosorbent Assay","url":"https://www.academia.edu/Documents/in/Enzyme_Linked_Immunosorbent_Assay"},{"id":1709309,"name":"Bovine Viral Diarrhea Virus","url":"https://www.academia.edu/Documents/in/Bovine_Viral_Diarrhea_Virus"},{"id":1793091,"name":"Buffaloes","url":"https://www.academia.edu/Documents/in/Buffaloes"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="17119379"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/17119379/Vaccination_trial_of_quail_with_herpes_virus_of_turkey"><img alt="Research paper thumbnail of Vaccination trial of quail with herpes virus of turkey" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/17119379/Vaccination_trial_of_quail_with_herpes_virus_of_turkey">Vaccination trial of quail with herpes virus of turkey</a></div><div class="wp-workCard_item"><span>Preventive Veterinary Medicine</span><span>, 1991</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Attempts were made to evaluate the efficacy of herpes virus of turkey (HVT) in the protection of ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Attempts were made to evaluate the efficacy of herpes virus of turkey (HVT) in the protection of quail against Marek&amp;#x27;s disease (MD). One hundred and four 1-day-old quail, each vaccinated with 1000 plaque-forming units (PFU) of HVT were challenged 10 days post-vaccination ...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="17119379"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="17119379"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 17119379; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=17119379]").text(description); $(".js-view-count[data-work-id=17119379]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 17119379; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='17119379']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 17119379, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=17119379]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":17119379,"title":"Vaccination trial of quail with herpes virus of turkey","translated_title":"","metadata":{"abstract":"Attempts were made to evaluate the efficacy of herpes virus of turkey (HVT) in the protection of quail against Marek\u0026amp;#x27;s disease (MD). One hundred and four 1-day-old quail, each vaccinated with 1000 plaque-forming units (PFU) of HVT were challenged 10 days post-vaccination ...","publication_date":{"day":null,"month":null,"year":1991,"errors":{}},"publication_name":"Preventive Veterinary Medicine"},"translated_abstract":"Attempts were made to evaluate the efficacy of herpes virus of turkey (HVT) in the protection of quail against Marek\u0026amp;#x27;s disease (MD). One hundred and four 1-day-old quail, each vaccinated with 1000 plaque-forming units (PFU) of HVT were challenged 10 days post-vaccination ...","internal_url":"https://www.academia.edu/17119379/Vaccination_trial_of_quail_with_herpes_virus_of_turkey","translated_internal_url":"","created_at":"2015-10-21T11:12:54.212-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":36693995,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":7717059,"work_id":17119379,"tagging_user_id":36693995,"tagged_user_id":235218309,"co_author_invite_id":1723467,"email":"l***l@howard.edu","display_order":0,"name":"Lalita Kaul","title":"Vaccination trial of quail with herpes virus of turkey"}],"downloadable_attachments":[],"slug":"Vaccination_trial_of_quail_with_herpes_virus_of_turkey","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":36693995,"first_name":"Hare","middle_initials":"","last_name":"Pradhan","page_name":"HarePradhan","domain_name":"independent","created_at":"2015-10-21T11:07:05.415-07:00","display_name":"Hare Pradhan","url":"https://independent.academia.edu/HarePradhan"},"attachments":[],"research_interests":[{"id":43819,"name":"Preventive Veterinary Medicine","url":"https://www.academia.edu/Documents/in/Preventive_Veterinary_Medicine"},{"id":644860,"name":"Veterinary Sciences","url":"https://www.academia.edu/Documents/in/Veterinary_Sciences"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="17119378"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/17119378/Single_chain_fragment_variable_antibody_against_the_capsid_protein_of_bovine_immunodeficiency_virus_and_its_use_in_ELISA"><img alt="Research paper thumbnail of Single-chain fragment variable antibody against the capsid protein of bovine immunodeficiency virus and its use in ELISA" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/17119378/Single_chain_fragment_variable_antibody_against_the_capsid_protein_of_bovine_immunodeficiency_virus_and_its_use_in_ELISA">Single-chain fragment variable antibody against the capsid protein of bovine immunodeficiency virus and its use in ELISA</a></div><div class="wp-workCard_item"><span>Journal of Virological Methods</span><span>, 2010</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Recombinant antibody specific for the capsid (CA) protein of bovine immunodeficiency virus (BIV) ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Recombinant antibody specific for the capsid (CA) protein of bovine immunodeficiency virus (BIV) was generated in the form of single-chain fragment variable (ScFv) using the phage display technique for affinity selection. The variable heavy (V(H)) and variable light (V(L)) chain gene fragments were recovered from cells of CA-specific hybridoma (9G10) described previously. The V(H) and V(L) DNA fragments were assembled through a flexible linker DNA to generate ScFv fragment which was cloned in a phagemid expression vector to express ScFv protein. The specific reactivity of the expressed ScFv to the CA antigen was confirmed by Western blot, and the ScFv fragment was used to develop a competitive inhibition ELISA for detection of antibodies to BIV in cattle and buffalo. The recombinant antibody was shown to be more than four times sensitive than its parent monoclonal antibody (MAb, 9G10) by testing of spiked samples of reference positive sera. The improved sensitivity of the recombinant antibody-based ELISA was confirmed by the detection of a larger proportion of animals with BIV antibody by it than by the MAb-based ELISA.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="17119378"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="17119378"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 17119378; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=17119378]").text(description); $(".js-view-count[data-work-id=17119378]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 17119378; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='17119378']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 17119378, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=17119378]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":17119378,"title":"Single-chain fragment variable antibody against the capsid protein of bovine immunodeficiency virus and its use in ELISA","translated_title":"","metadata":{"abstract":"Recombinant antibody specific for the capsid (CA) protein of bovine immunodeficiency virus (BIV) was generated in the form of single-chain fragment variable (ScFv) using the phage display technique for affinity selection. The variable heavy (V(H)) and variable light (V(L)) chain gene fragments were recovered from cells of CA-specific hybridoma (9G10) described previously. The V(H) and V(L) DNA fragments were assembled through a flexible linker DNA to generate ScFv fragment which was cloned in a phagemid expression vector to express ScFv protein. The specific reactivity of the expressed ScFv to the CA antigen was confirmed by Western blot, and the ScFv fragment was used to develop a competitive inhibition ELISA for detection of antibodies to BIV in cattle and buffalo. The recombinant antibody was shown to be more than four times sensitive than its parent monoclonal antibody (MAb, 9G10) by testing of spiked samples of reference positive sera. The improved sensitivity of the recombinant antibody-based ELISA was confirmed by the detection of a larger proportion of animals with BIV antibody by it than by the MAb-based ELISA.","publication_date":{"day":null,"month":null,"year":2010,"errors":{}},"publication_name":"Journal of Virological Methods"},"translated_abstract":"Recombinant antibody specific for the capsid (CA) protein of bovine immunodeficiency virus (BIV) was generated in the form of single-chain fragment variable (ScFv) using the phage display technique for affinity selection. The variable heavy (V(H)) and variable light (V(L)) chain gene fragments were recovered from cells of CA-specific hybridoma (9G10) described previously. The V(H) and V(L) DNA fragments were assembled through a flexible linker DNA to generate ScFv fragment which was cloned in a phagemid expression vector to express ScFv protein. The specific reactivity of the expressed ScFv to the CA antigen was confirmed by Western blot, and the ScFv fragment was used to develop a competitive inhibition ELISA for detection of antibodies to BIV in cattle and buffalo. The recombinant antibody was shown to be more than four times sensitive than its parent monoclonal antibody (MAb, 9G10) by testing of spiked samples of reference positive sera. The improved sensitivity of the recombinant antibody-based ELISA was confirmed by the detection of a larger proportion of animals with BIV antibody by it than by the MAb-based ELISA.","internal_url":"https://www.academia.edu/17119378/Single_chain_fragment_variable_antibody_against_the_capsid_protein_of_bovine_immunodeficiency_virus_and_its_use_in_ELISA","translated_internal_url":"","created_at":"2015-10-21T11:12:54.080-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":36693995,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":7717049,"work_id":17119378,"tagging_user_id":36693995,"tagged_user_id":null,"co_author_invite_id":1723465,"email":"b***n@yahoo.co.in","display_order":0,"name":"S. Bhatia","title":"Single-chain fragment variable antibody against the capsid protein of bovine immunodeficiency virus and its use in ELISA"},{"id":7717053,"work_id":17119378,"tagging_user_id":36693995,"tagged_user_id":29844908,"co_author_invite_id":null,"email":"r***d@icar.gov.in","display_order":4194304,"name":"Richa Sood","title":"Single-chain fragment variable antibody against the capsid protein of bovine immunodeficiency virus and its use in ELISA"},{"id":7717056,"work_id":17119378,"tagging_user_id":36693995,"tagged_user_id":54411413,"co_author_invite_id":1723466,"email":"r***l@gmail.com","affiliation":"Nanaji Deshmukh Veterinary Science University. Jabalpur","display_order":6291456,"name":"Rakhi Gangil","title":"Single-chain fragment variable antibody against the capsid protein of bovine immunodeficiency virus and its use in ELISA"},{"id":7717057,"work_id":17119378,"tagging_user_id":36693995,"tagged_user_id":2395666,"co_author_invite_id":null,"email":"d***4@gmail.com","affiliation":"O.P. Jindal Global University","display_order":7340032,"name":"Devyani Gupta","title":"Single-chain fragment variable antibody against the capsid protein of bovine immunodeficiency virus and its use in ELISA"},{"id":7717058,"work_id":17119378,"tagging_user_id":36693995,"tagged_user_id":18520014,"co_author_invite_id":null,"email":"s***6@gmail.com","affiliation":"Banaras Hindu University, Varanasi","display_order":7864320,"name":"SANDEEP KUMAR DUBEY","title":"Single-chain fragment variable antibody against the capsid protein of bovine immunodeficiency virus and its use in ELISA"}],"downloadable_attachments":[],"slug":"Single_chain_fragment_variable_antibody_against_the_capsid_protein_of_bovine_immunodeficiency_virus_and_its_use_in_ELISA","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":36693995,"first_name":"Hare","middle_initials":"","last_name":"Pradhan","page_name":"HarePradhan","domain_name":"independent","created_at":"2015-10-21T11:07:05.415-07:00","display_name":"Hare Pradhan","url":"https://independent.academia.edu/HarePradhan"},"attachments":[],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology"},{"id":6947,"name":"Medical Microbiology","url":"https://www.academia.edu/Documents/in/Medical_Microbiology"},{"id":8089,"name":"Virology","url":"https://www.academia.edu/Documents/in/Virology"},{"id":43838,"name":"India","url":"https://www.academia.edu/Documents/in/India"},{"id":130513,"name":"Phage display","url":"https://www.academia.edu/Documents/in/Phage_display"},{"id":216801,"name":"Journal of Virological Methods","url":"https://www.academia.edu/Documents/in/Journal_of_Virological_Methods"},{"id":260829,"name":"Cattle","url":"https://www.academia.edu/Documents/in/Cattle"},{"id":462111,"name":"Western blot","url":"https://www.academia.edu/Documents/in/Western_blot"},{"id":766014,"name":"Monoclonal Antibody","url":"https://www.academia.edu/Documents/in/Monoclonal_Antibody"},{"id":901876,"name":"Sensitivity and Specificity","url":"https://www.academia.edu/Documents/in/Sensitivity_and_Specificity"},{"id":1248637,"name":"Capsid Protein","url":"https://www.academia.edu/Documents/in/Capsid_Protein"},{"id":1272906,"name":"Enzyme Linked Immunosorbent Assay","url":"https://www.academia.edu/Documents/in/Enzyme_Linked_Immunosorbent_Assay"},{"id":1793091,"name":"Buffaloes","url":"https://www.academia.edu/Documents/in/Buffaloes"},{"id":2256666,"name":"DNA fragmentation","url":"https://www.academia.edu/Documents/in/DNA_fragmentation"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="17119377"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/17119377/Development_of_a_capsid_based_competitive_inhibition_enzyme_linked_immunosorbent_assay_for_detection_of_bovine_immunodeficiency_virus_antibodies_in_cattle_and_buffalo_serum"><img alt="Research paper thumbnail of Development of a capsid based competitive inhibition enzyme-linked immunosorbent assay for detection of bovine immunodeficiency virus antibodies in cattle and buffalo serum" class="work-thumbnail" src="https://attachments.academia-assets.com/42316771/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/17119377/Development_of_a_capsid_based_competitive_inhibition_enzyme_linked_immunosorbent_assay_for_detection_of_bovine_immunodeficiency_virus_antibodies_in_cattle_and_buffalo_serum">Development of a capsid based competitive inhibition enzyme-linked immunosorbent assay for detection of bovine immunodeficiency virus antibodies in cattle and buffalo serum</a></div><div class="wp-workCard_item"><span>Journal of Virological Methods</span><span>, 2008</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="8cc3f5e855d86d56b6e076a53d992dee" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":42316771,"asset_id":17119377,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/42316771/download_file?st=MTczMzE5NzgyMSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="17119377"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="17119377"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 17119377; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=17119377]").text(description); $(".js-view-count[data-work-id=17119377]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 17119377; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='17119377']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 17119377, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "8cc3f5e855d86d56b6e076a53d992dee" } } $('.js-work-strip[data-work-id=17119377]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":17119377,"title":"Development of a capsid based competitive inhibition enzyme-linked immunosorbent assay for detection of bovine immunodeficiency virus antibodies in cattle and buffalo serum","translated_title":"","metadata":{"grobid_abstract":"The aim of this study was to develop a more specific and sensitive competitive inhibition ELISA (CI-ELISA) than the currently used indirect ELISA for detection of antibodies to bovine immunodeficiency virus (BIV) in cattle and buffaloes. Murine monoclonal antibodies (MAbs) were generated against a recombinant capsid (CA) protein of bovine immunodeficiency virus. Of the 13 anti-CA MAbs developed, MAb-9G10 was selected for CI-ELISA based on the maximum inhibition (98%) obtained with reference BIV antibody positive serum. Based on the distribution of percent inhibition of known negative sera (n = 50), a cut-off value was set at 40% inhibition. The MAb-based CI-ELISA showed much higher agreement (concordance: 95.4%) than the indirect ELISA (concordance: 77.8%) with Western blot. Out of 672 sera of cattle and buffaloes tested by CI-ELISA from four states of India, 22% (113/516) of cattle and 19% (30/156) of buffalo were sero-positive for BIV with an overall seroprevalence of 21% (143/672) in India.","publication_date":{"day":null,"month":null,"year":2008,"errors":{}},"publication_name":"Journal of Virological Methods","grobid_abstract_attachment_id":42316771},"translated_abstract":null,"internal_url":"https://www.academia.edu/17119377/Development_of_a_capsid_based_competitive_inhibition_enzyme_linked_immunosorbent_assay_for_detection_of_bovine_immunodeficiency_virus_antibodies_in_cattle_and_buffalo_serum","translated_internal_url":"","created_at":"2015-10-21T11:12:53.966-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":36693995,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":7717018,"work_id":17119377,"tagging_user_id":36693995,"tagged_user_id":null,"co_author_invite_id":124581,"email":"p***b@gmail.com","display_order":0,"name":"B. Pattnaik","title":"Development of a capsid based competitive inhibition enzyme-linked immunosorbent assay for detection of bovine immunodeficiency virus antibodies in cattle and buffalo serum"},{"id":7717048,"work_id":17119377,"tagging_user_id":36693995,"tagged_user_id":null,"co_author_invite_id":1723465,"email":"b***n@yahoo.co.in","display_order":4194304,"name":"S. Bhatia","title":"Development of a capsid based competitive inhibition enzyme-linked immunosorbent assay for detection of bovine immunodeficiency virus antibodies in cattle and buffalo serum"},{"id":7717051,"work_id":17119377,"tagging_user_id":36693995,"tagged_user_id":29844908,"co_author_invite_id":null,"email":"r***d@icar.gov.in","display_order":6291456,"name":"Richa Sood","title":"Development of a capsid based competitive inhibition enzyme-linked immunosorbent assay for detection of bovine immunodeficiency virus antibodies in cattle and buffalo serum"},{"id":7717055,"work_id":17119377,"tagging_user_id":36693995,"tagged_user_id":5700610,"co_author_invite_id":null,"email":"a***3@yahoo.com","display_order":7340032,"name":"Ashok Bhatia","title":"Development of a capsid based competitive inhibition enzyme-linked immunosorbent assay for detection of bovine immunodeficiency virus antibodies in cattle and buffalo serum"}],"downloadable_attachments":[{"id":42316771,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/42316771/thumbnails/1.jpg","file_name":"Development_of_a_capsid_based_competitiv20160207-11015-mky6he.pdf","download_url":"https://www.academia.edu/attachments/42316771/download_file?st=MTczMzE5NzgyMSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Development_of_a_capsid_based_competitiv.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/42316771/Development_of_a_capsid_based_competitiv20160207-11015-mky6he-libre.pdf?1454868700=\u0026response-content-disposition=attachment%3B+filename%3DDevelopment_of_a_capsid_based_competitiv.pdf\u0026Expires=1733201421\u0026Signature=LdMqk6EE~JLbsQhnDUrE1pxJuB4y9192HCqQA9ABEJRfOk9RgZGslc1k1S9OQ0dlDqT6vvanSJIGNcvAAoORcDEC0gHciUTfdpbpct--UdgsV947C7d9UTcZ9PQmLo-HXe8LaR2VA80MCRgfmhNyr7JGEhH~pLM9NVwEontIu67sUiU~jGt8S8bgAR5Q6LM-Y8cXnpdtnRrrofNQV6t0dyfj4JsZQdLJFWkUIiQweATjDHVXBh9qgcOhxUToG1Rhf0RtycPynZrR6Kv95E79gtBJam5Jr52PeAU1SMc1LcCrb4r9uNDb8WzJrJ9seko7pZF6DcxvxIpA7mmmFgdBfA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Development_of_a_capsid_based_competitive_inhibition_enzyme_linked_immunosorbent_assay_for_detection_of_bovine_immunodeficiency_virus_antibodies_in_cattle_and_buffalo_serum","translated_slug":"","page_count":8,"language":"en","content_type":"Work","owner":{"id":36693995,"first_name":"Hare","middle_initials":"","last_name":"Pradhan","page_name":"HarePradhan","domain_name":"independent","created_at":"2015-10-21T11:07:05.415-07:00","display_name":"Hare Pradhan","url":"https://independent.academia.edu/HarePradhan"},"attachments":[{"id":42316771,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/42316771/thumbnails/1.jpg","file_name":"Development_of_a_capsid_based_competitiv20160207-11015-mky6he.pdf","download_url":"https://www.academia.edu/attachments/42316771/download_file?st=MTczMzE5NzgyMSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Development_of_a_capsid_based_competitiv.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/42316771/Development_of_a_capsid_based_competitiv20160207-11015-mky6he-libre.pdf?1454868700=\u0026response-content-disposition=attachment%3B+filename%3DDevelopment_of_a_capsid_based_competitiv.pdf\u0026Expires=1733201421\u0026Signature=LdMqk6EE~JLbsQhnDUrE1pxJuB4y9192HCqQA9ABEJRfOk9RgZGslc1k1S9OQ0dlDqT6vvanSJIGNcvAAoORcDEC0gHciUTfdpbpct--UdgsV947C7d9UTcZ9PQmLo-HXe8LaR2VA80MCRgfmhNyr7JGEhH~pLM9NVwEontIu67sUiU~jGt8S8bgAR5Q6LM-Y8cXnpdtnRrrofNQV6t0dyfj4JsZQdLJFWkUIiQweATjDHVXBh9qgcOhxUToG1Rhf0RtycPynZrR6Kv95E79gtBJam5Jr52PeAU1SMc1LcCrb4r9uNDb8WzJrJ9seko7pZF6DcxvxIpA7mmmFgdBfA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology"},{"id":6947,"name":"Medical Microbiology","url":"https://www.academia.edu/Documents/in/Medical_Microbiology"},{"id":37834,"name":"Western blotting","url":"https://www.academia.edu/Documents/in/Western_blotting"},{"id":43838,"name":"India","url":"https://www.academia.edu/Documents/in/India"},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice"},{"id":147196,"name":"Monoclonal Antibodies","url":"https://www.academia.edu/Documents/in/Monoclonal_Antibodies"},{"id":216801,"name":"Journal of Virological Methods","url":"https://www.academia.edu/Documents/in/Journal_of_Virological_Methods"},{"id":260829,"name":"Cattle","url":"https://www.academia.edu/Documents/in/Cattle"},{"id":462111,"name":"Western blot","url":"https://www.academia.edu/Documents/in/Western_blot"},{"id":766014,"name":"Monoclonal Antibody","url":"https://www.academia.edu/Documents/in/Monoclonal_Antibody"},{"id":1272906,"name":"Enzyme Linked Immunosorbent Assay","url":"https://www.academia.edu/Documents/in/Enzyme_Linked_Immunosorbent_Assay"},{"id":1793091,"name":"Buffaloes","url":"https://www.academia.edu/Documents/in/Buffaloes"}],"urls":[{"id":6522419,"url":"https://www.researchgate.net/profile/Sandeep_Bhatia3/publication/5659596_Development_of_a_capsid_based_competitive_inhibition_enzyme-linked_immunosorbent_assay_for_detection_of_bovine_immunodeficiency_virus_antibodies_in_cattle_and_buffalo_serum/links/0deec52972f6b52c50000000.pdf"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="17119376"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/17119376/Development_and_evaluation_of_SYBR_Green_I_based_one_step_real_time_RT_PCR_assay_for_detection_and_quantification_of_Chikungunya_virus"><img alt="Research paper thumbnail of Development and evaluation of SYBR Green I-based one-step real-time RT-PCR assay for detection and quantification of Chikungunya virus" class="work-thumbnail" src="https://attachments.academia-assets.com/42316777/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/17119376/Development_and_evaluation_of_SYBR_Green_I_based_one_step_real_time_RT_PCR_assay_for_detection_and_quantification_of_Chikungunya_virus">Development and evaluation of SYBR Green I-based one-step real-time RT-PCR assay for detection and quantification of Chikungunya virus</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/HarePradhan">Hare Pradhan</a> and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/PVLakshmanaRao">P. V. Lakshmana Rao</a></span></div><div class="wp-workCard_item"><span>Journal of Clinical Virology</span><span>, 2007</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="95865b56f50a494e3e27f7884e5cd224" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":42316777,"asset_id":17119376,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/42316777/download_file?st=MTczMzE5NzgyMSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="17119376"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="17119376"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 17119376; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=17119376]").text(description); $(".js-view-count[data-work-id=17119376]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 17119376; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='17119376']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 17119376, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "95865b56f50a494e3e27f7884e5cd224" } } $('.js-work-strip[data-work-id=17119376]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":17119376,"title":"Development and evaluation of SYBR Green I-based one-step real-time RT-PCR assay for detection and quantification of Chikungunya virus","translated_title":"","metadata":{"grobid_abstract":"The development of a one-step SYBR Green I-based real-time RT-PCR assay is reported for detection and quantification of Chikungunya virus (CHIKV) in acute-phase patient serum samples by targeting the E1 structural gene. A linear relationship was obtained between the virus concentration and cycle threshold (C t ) value over a range of 10 7 -0.1 PFU/ml. The reported assay was found to be 10-fold more sensitive compared to conventional RT-PCR with a detection limit of 0.1 PFU/ml. The feasibility of this reported assay system for clinical diagnosis was validated with 51 suspected acute-phase serum samples of the recent CHIKV epidemic in southern India, 2006. The comparative evaluation with acute-phase patient serum samples revealed the higher sensitivity of real-time RT-PCR assay by picking up six additional samples with low copy number of template. None of the healthy serum samples analyzed in this study showed amplification. The quantification of the viral load in the acute-phase serum samples was also determined employing the standard curve, which varies from 0.1 to 10 7 PFU/ml. These findings demonstrated that the reported assay has the potential usefulness for clinical diagnosis due to simultaneous detection and quantification of Chikungunya virus in acute-phase patient serum samples.","publication_date":{"day":null,"month":null,"year":2007,"errors":{}},"publication_name":"Journal of Clinical Virology","grobid_abstract_attachment_id":42316777},"translated_abstract":null,"internal_url":"https://www.academia.edu/17119376/Development_and_evaluation_of_SYBR_Green_I_based_one_step_real_time_RT_PCR_assay_for_detection_and_quantification_of_Chikungunya_virus","translated_internal_url":"","created_at":"2015-10-21T11:12:53.855-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":36693995,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":7717020,"work_id":17119376,"tagging_user_id":36693995,"tagged_user_id":null,"co_author_invite_id":124581,"email":"p***b@gmail.com","display_order":0,"name":"B. Pattnaik","title":"Development and evaluation of SYBR Green I-based one-step real-time RT-PCR assay for detection and quantification of Chikungunya virus"},{"id":7717039,"work_id":17119376,"tagging_user_id":36693995,"tagged_user_id":18540878,"co_author_invite_id":null,"email":"s***h@gmail.com","display_order":4194304,"name":"Santhosh Sundaresan","title":"Development and evaluation of SYBR Green I-based one-step real-time RT-PCR assay for detection and quantification of Chikungunya virus"},{"id":7717040,"work_id":17119376,"tagging_user_id":36693995,"tagged_user_id":null,"co_author_invite_id":688053,"email":"p***m@rediffmail.com","display_order":6291456,"name":"M. Parida","title":"Development and evaluation of SYBR Green I-based one-step real-time RT-PCR assay for detection and quantification of Chikungunya virus"},{"id":7717041,"work_id":17119376,"tagging_user_id":36693995,"tagged_user_id":null,"co_author_invite_id":56134,"email":"p***h@rediffmail.com","display_order":7340032,"name":"P. Dash","title":"Development and evaluation of SYBR Green I-based one-step real-time RT-PCR assay for detection and quantification of Chikungunya virus"},{"id":7717042,"work_id":17119376,"tagging_user_id":36693995,"tagged_user_id":61485251,"co_author_invite_id":1723462,"email":"a***a@gmail.com","display_order":7864320,"name":"atul pateriya","title":"Development and evaluation of SYBR Green I-based one-step real-time RT-PCR assay for detection and quantification of Chikungunya virus"},{"id":7717043,"work_id":17119376,"tagging_user_id":36693995,"tagged_user_id":null,"co_author_invite_id":1723463,"email":"a***p@gmail.com","display_order":8126464,"name":"S. Ambuj","title":"Development and evaluation of SYBR Green I-based one-step real-time RT-PCR assay for detection and quantification of Chikungunya virus"},{"id":7717044,"work_id":17119376,"tagging_user_id":36693995,"tagged_user_id":null,"co_author_invite_id":1530203,"email":"n***a@crc.jussieu.fr","display_order":8257536,"name":"N. Gupta","title":"Development and evaluation of SYBR Green I-based one-step real-time RT-PCR assay for detection and quantification of Chikungunya virus"},{"id":7717045,"work_id":17119376,"tagging_user_id":36693995,"tagged_user_id":27069456,"co_author_invite_id":null,"email":"p***a@gmail.com","display_order":8323072,"name":"praveen saxena","title":"Development and evaluation of SYBR Green I-based one-step real-time RT-PCR assay for detection and quantification of Chikungunya virus"},{"id":7717046,"work_id":17119376,"tagging_user_id":36693995,"tagged_user_id":1389695,"co_author_invite_id":null,"email":"p***1@gmail.com","affiliation":"National Institute of Technology, Warangal","display_order":8355840,"name":"Vengal Rao Pachava","title":"Development and evaluation of SYBR Green I-based one-step real-time RT-PCR assay for detection and quantification of Chikungunya virus"},{"id":7717047,"work_id":17119376,"tagging_user_id":36693995,"tagged_user_id":38024303,"co_author_invite_id":1723464,"email":"p***o@rediffmail.com","display_order":8372224,"name":"P. V. Lakshmana Rao","title":"Development and evaluation of SYBR Green I-based one-step real-time RT-PCR assay for detection and quantification of Chikungunya virus"}],"downloadable_attachments":[{"id":42316777,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/42316777/thumbnails/1.jpg","file_name":"j.jcv.2007.04.015.pdf20160207-9459-1rrgo77","download_url":"https://www.academia.edu/attachments/42316777/download_file?st=MTczMzE5NzgyMSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Development_and_evaluation_of_SYBR_Green.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/42316777/j.jcv.2007.04.015-libre.pdf20160207-9459-1rrgo77?1454868700=\u0026response-content-disposition=attachment%3B+filename%3DDevelopment_and_evaluation_of_SYBR_Green.pdf\u0026Expires=1733170423\u0026Signature=Icopv9i-rJ8yP8xu~~dzINVAYmXn2DUV8CRwCJ~Rklu0vApOJw70XWdiWg~sKv1GEOIQA4u6OtjjKdCD0cuGlC1fGm9zHjMqkMDrSpOtaNr6dLvY0EvsNYgXOYoe-g0Vy~imo8y-BV0vQUdz5u2HEnQzcb8gizaz9btDCniPenPFzo9jYPCKNWgJKMz6Wl9AhahUUen7r0~Iq0oFHyyFoGlNyxz-cqCKcqnNnepUoTRx3RrYu6lIQ8OqezQU-CKD5RZhkXNlt2ImrAD7Z1LGu6okRig2E7ZQdPo0Zl2Te5xXPKDKW8ylg-y5so~GPOKuuETGI9XjouJAGPF8UscKNQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Development_and_evaluation_of_SYBR_Green_I_based_one_step_real_time_RT_PCR_assay_for_detection_and_quantification_of_Chikungunya_virus","translated_slug":"","page_count":6,"language":"en","content_type":"Work","owner":{"id":36693995,"first_name":"Hare","middle_initials":"","last_name":"Pradhan","page_name":"HarePradhan","domain_name":"independent","created_at":"2015-10-21T11:07:05.415-07:00","display_name":"Hare Pradhan","url":"https://independent.academia.edu/HarePradhan"},"attachments":[{"id":42316777,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/42316777/thumbnails/1.jpg","file_name":"j.jcv.2007.04.015.pdf20160207-9459-1rrgo77","download_url":"https://www.academia.edu/attachments/42316777/download_file?st=MTczMzE5NzgyMSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Development_and_evaluation_of_SYBR_Green.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/42316777/j.jcv.2007.04.015-libre.pdf20160207-9459-1rrgo77?1454868700=\u0026response-content-disposition=attachment%3B+filename%3DDevelopment_and_evaluation_of_SYBR_Green.pdf\u0026Expires=1733170423\u0026Signature=Icopv9i-rJ8yP8xu~~dzINVAYmXn2DUV8CRwCJ~Rklu0vApOJw70XWdiWg~sKv1GEOIQA4u6OtjjKdCD0cuGlC1fGm9zHjMqkMDrSpOtaNr6dLvY0EvsNYgXOYoe-g0Vy~imo8y-BV0vQUdz5u2HEnQzcb8gizaz9btDCniPenPFzo9jYPCKNWgJKMz6Wl9AhahUUen7r0~Iq0oFHyyFoGlNyxz-cqCKcqnNnepUoTRx3RrYu6lIQ8OqezQU-CKD5RZhkXNlt2ImrAD7Z1LGu6okRig2E7ZQdPo0Zl2Te5xXPKDKW8ylg-y5so~GPOKuuETGI9XjouJAGPF8UscKNQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology"},{"id":6947,"name":"Medical Microbiology","url":"https://www.academia.edu/Documents/in/Medical_Microbiology"},{"id":18529,"name":"Fluorescent Dyes and Reagents","url":"https://www.academia.edu/Documents/in/Fluorescent_Dyes_and_Reagents"},{"id":37833,"name":"Real-time RT-PCR","url":"https://www.academia.edu/Documents/in/Real-time_RT-PCR"},{"id":43838,"name":"India","url":"https://www.academia.edu/Documents/in/India"},{"id":49785,"name":"Clinical Virology","url":"https://www.academia.edu/Documents/in/Clinical_Virology"},{"id":51784,"name":"real time PCR","url":"https://www.academia.edu/Documents/in/real_time_PCR"},{"id":99708,"name":"Clinical","url":"https://www.academia.edu/Documents/in/Clinical"},{"id":210074,"name":"Japanese encephalitis","url":"https://www.academia.edu/Documents/in/Japanese_encephalitis"},{"id":216801,"name":"Journal of Virological Methods","url":"https://www.academia.edu/Documents/in/Journal_of_Virological_Methods"},{"id":229390,"name":"Real Time","url":"https://www.academia.edu/Documents/in/Real_Time"},{"id":244814,"name":"Clinical Sciences","url":"https://www.academia.edu/Documents/in/Clinical_Sciences"},{"id":709507,"name":"Diagnostic Test","url":"https://www.academia.edu/Documents/in/Diagnostic_Test"},{"id":784076,"name":"Species Specificity","url":"https://www.academia.edu/Documents/in/Species_Specificity"},{"id":877165,"name":"Chikungunya Virus","url":"https://www.academia.edu/Documents/in/Chikungunya_Virus"},{"id":901876,"name":"Sensitivity and Specificity","url":"https://www.academia.edu/Documents/in/Sensitivity_and_Specificity"},{"id":1193376,"name":"Japanese Encephalitis Virus","url":"https://www.academia.edu/Documents/in/Japanese_Encephalitis_Virus"},{"id":1275886,"name":"Clinical Application","url":"https://www.academia.edu/Documents/in/Clinical_Application"},{"id":1297608,"name":"Organic Chemicals","url":"https://www.academia.edu/Documents/in/Organic_Chemicals"},{"id":1434202,"name":"Clinical Diagnosis","url":"https://www.academia.edu/Documents/in/Clinical_Diagnosis"},{"id":2249317,"name":"Viral Load","url":"https://www.academia.edu/Documents/in/Viral_Load"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="17119375"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/17119375/Genetic_Analysis_and_Expression_of_NS3_Gene_of_Bovine_Viral_Diarrhoea_Virus_1_from_India_for_Detection_of_Antibodies_in_Cattle"><img alt="Research paper thumbnail of Genetic Analysis and Expression of NS3 Gene of Bovine Viral Diarrhoea Virus 1 from India for Detection of Antibodies in Cattle" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/17119375/Genetic_Analysis_and_Expression_of_NS3_Gene_of_Bovine_Viral_Diarrhoea_Virus_1_from_India_for_Detection_of_Antibodies_in_Cattle">Genetic Analysis and Expression of NS3 Gene of Bovine Viral Diarrhoea Virus 1 from India for Detection of Antibodies in Cattle</a></div><div class="wp-workCard_item"><span>Journal of Applied Animal Research</span><span>, 2008</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Mishra, N., Pitale, S.S. and Pradhan, H.K. 2008. Genetic analysis and expression of NS3 gene of b...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Mishra, N., Pitale, S.S. and Pradhan, H.K. 2008. Genetic analysis and expression of NS3 gene of bovine viral diarrhoea virus 1 from India for detection of antibodies in cattle. J. Appl. Anim. Res., 33: 99–103.Considering the importance of NS3 antigen in diagnosis of bovine viral diarrhea virus (BVDV) infection, we analysed genetically selected Indian isolates in NS3 gene region and</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="17119375"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="17119375"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 17119375; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=17119375]").text(description); $(".js-view-count[data-work-id=17119375]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 17119375; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='17119375']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 17119375, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=17119375]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":17119375,"title":"Genetic Analysis and Expression of NS3 Gene of Bovine Viral Diarrhoea Virus 1 from India for Detection of Antibodies in Cattle","translated_title":"","metadata":{"abstract":"Mishra, N., Pitale, S.S. and Pradhan, H.K. 2008. Genetic analysis and expression of NS3 gene of bovine viral diarrhoea virus 1 from India for detection of antibodies in cattle. J. Appl. Anim. Res., 33: 99–103.Considering the importance of NS3 antigen in diagnosis of bovine viral diarrhea virus (BVDV) infection, we analysed genetically selected Indian isolates in NS3 gene region and","publication_date":{"day":null,"month":null,"year":2008,"errors":{}},"publication_name":"Journal of Applied Animal Research"},"translated_abstract":"Mishra, N., Pitale, S.S. and Pradhan, H.K. 2008. Genetic analysis and expression of NS3 gene of bovine viral diarrhoea virus 1 from India for detection of antibodies in cattle. J. Appl. Anim. Res., 33: 99–103.Considering the importance of NS3 antigen in diagnosis of bovine viral diarrhea virus (BVDV) infection, we analysed genetically selected Indian isolates in NS3 gene region and","internal_url":"https://www.academia.edu/17119375/Genetic_Analysis_and_Expression_of_NS3_Gene_of_Bovine_Viral_Diarrhoea_Virus_1_from_India_for_Detection_of_Antibodies_in_Cattle","translated_internal_url":"","created_at":"2015-10-21T11:12:53.732-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":36693995,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":7717031,"work_id":17119375,"tagging_user_id":36693995,"tagged_user_id":50823026,"co_author_invite_id":616470,"email":"m***r@rediffmail.com","display_order":0,"name":"Niranjan Mishra","title":"Genetic Analysis and Expression of NS3 Gene of Bovine Viral Diarrhoea Virus 1 from India for Detection of Antibodies in Cattle"},{"id":7717036,"work_id":17119375,"tagging_user_id":36693995,"tagged_user_id":1175172,"co_author_invite_id":null,"email":"s***9@gmail.com","display_order":4194304,"name":"Shreyas Pitale","title":"Genetic Analysis and Expression of NS3 Gene of Bovine Viral Diarrhoea Virus 1 from India for Detection of Antibodies in Cattle"}],"downloadable_attachments":[],"slug":"Genetic_Analysis_and_Expression_of_NS3_Gene_of_Bovine_Viral_Diarrhoea_Virus_1_from_India_for_Detection_of_Antibodies_in_Cattle","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":36693995,"first_name":"Hare","middle_initials":"","last_name":"Pradhan","page_name":"HarePradhan","domain_name":"independent","created_at":"2015-10-21T11:07:05.415-07:00","display_name":"Hare Pradhan","url":"https://independent.academia.edu/HarePradhan"},"attachments":[],"research_interests":[{"id":173,"name":"Zoology","url":"https://www.academia.edu/Documents/in/Zoology"},{"id":29980,"name":"Animal Production","url":"https://www.academia.edu/Documents/in/Animal_Production"},{"id":38884,"name":"Applied Animal Behavior","url":"https://www.academia.edu/Documents/in/Applied_Animal_Behavior"},{"id":83128,"name":"Escherichia coli","url":"https://www.academia.edu/Documents/in/Escherichia_coli"},{"id":284067,"name":"Inclusion Bodies","url":"https://www.academia.edu/Documents/in/Inclusion_Bodies"},{"id":462111,"name":"Western blot","url":"https://www.academia.edu/Documents/in/Western_blot"},{"id":707213,"name":"Genetic Analysis","url":"https://www.academia.edu/Documents/in/Genetic_Analysis"},{"id":809881,"name":"Amino Acid Sequence","url":"https://www.academia.edu/Documents/in/Amino_Acid_Sequence"},{"id":959510,"name":"Recombinant Protein","url":"https://www.academia.edu/Documents/in/Recombinant_Protein"},{"id":1387364,"name":"Genetic Selection","url":"https://www.academia.edu/Documents/in/Genetic_Selection"},{"id":1597877,"name":"Nucleotides","url":"https://www.academia.edu/Documents/in/Nucleotides"},{"id":1709309,"name":"Bovine Viral Diarrhea Virus","url":"https://www.academia.edu/Documents/in/Bovine_Viral_Diarrhea_Virus"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="17119374"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/17119374/Rapid_Detection_of_Highly_Pathogenic_Avian_Influenza_H5N1_Virus_by_TaqMan_Reverse_Transcriptase_Polymerase_Chain_Reaction"><img alt="Research paper thumbnail of Rapid Detection of Highly Pathogenic Avian Influenza H5N1 Virus by TaqMan Reverse Transcriptase–Polymerase Chain Reaction" class="work-thumbnail" src="https://attachments.academia-assets.com/42316756/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/17119374/Rapid_Detection_of_Highly_Pathogenic_Avian_Influenza_H5N1_Virus_by_TaqMan_Reverse_Transcriptase_Polymerase_Chain_Reaction">Rapid Detection of Highly Pathogenic Avian Influenza H5N1 Virus by TaqMan Reverse Transcriptase–Polymerase Chain Reaction</a></div><div class="wp-workCard_item"><span>Avian Diseases</span><span>, 2007</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="d433cfaffdfbe1178853a229f15b800e" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":42316756,"asset_id":17119374,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/42316756/download_file?st=MTczMzE5NzgyMSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="17119374"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="17119374"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 17119374; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=17119374]").text(description); $(".js-view-count[data-work-id=17119374]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 17119374; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='17119374']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 17119374, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "d433cfaffdfbe1178853a229f15b800e" } } $('.js-work-strip[data-work-id=17119374]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":17119374,"title":"Rapid Detection of Highly Pathogenic Avian Influenza H5N1 Virus by TaqMan Reverse Transcriptase–Polymerase Chain Reaction","translated_title":"","metadata":{"grobid_abstract":"Highly pathogenic Avian Influenza (AI) H5N1 viruses have been spreading from Asia since late 2003. Early detection and classification are paramount for control of the disease because these viruses are lethal to birds and have caused fatalities in humans. Here we describe a TaqMan Reverse Transcriptase-Polymerase Chain Reaction Assay for rapid detection of Avian Influenza virus and for H5 subtyping by targeting HA gene of AI viruses. The assay was highly sensitive than RT-PCR and virus isolation in chick embryos. In the present study all samples (field samples) which are positive for HI and RT-PCR were tested by using TaqMan Reverse Transcriptase-Polymerase Chain Reaction Assay for reconfirmation. AI viruses (H5N1) were detected from nine samples which are received from Maharashtra during Avian influenza outbreak in India in 2006. Real-Time PCR assays was also conducted for detection of viral genome in different organs of experimental infected chickens revealed presence of the virus in all organs with high virus concentration in brain, heart, intestine and cloaca. This test allows definitive confirmation of an AI virus as H5 within hours, which is crucial for rapid implementation of control measures in the event of an outbreak.","publication_date":{"day":null,"month":null,"year":2007,"errors":{}},"publication_name":"Avian Diseases","grobid_abstract_attachment_id":42316756},"translated_abstract":null,"internal_url":"https://www.academia.edu/17119374/Rapid_Detection_of_Highly_Pathogenic_Avian_Influenza_H5N1_Virus_by_TaqMan_Reverse_Transcriptase_Polymerase_Chain_Reaction","translated_internal_url":"","created_at":"2015-10-21T11:12:53.624-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":36693995,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":7717017,"work_id":17119374,"tagging_user_id":36693995,"tagged_user_id":null,"co_author_invite_id":1723458,"email":"m***1@gmail.com","display_order":0,"name":"B. Shankar","title":"Rapid Detection of Highly Pathogenic Avian Influenza H5N1 Virus by TaqMan Reverse Transcriptase–Polymerase Chain Reaction"},{"id":7717021,"work_id":17119374,"tagging_user_id":36693995,"tagged_user_id":null,"co_author_invite_id":124581,"email":"p***b@gmail.com","display_order":4194304,"name":"B. Pattnaik","title":"Rapid Detection of Highly Pathogenic Avian Influenza H5N1 Virus by TaqMan Reverse Transcriptase–Polymerase Chain Reaction"},{"id":7717024,"work_id":17119374,"tagging_user_id":36693995,"tagged_user_id":160479623,"co_author_invite_id":1723459,"email":"b***i@gmail.com","display_order":6291456,"name":"BL Manjunath","title":"Rapid Detection of Highly Pathogenic Avian Influenza H5N1 Virus by TaqMan Reverse Transcriptase–Polymerase Chain Reaction"},{"id":7717026,"work_id":17119374,"tagging_user_id":36693995,"tagged_user_id":7223595,"co_author_invite_id":null,"email":"d***l@gmail.com","display_order":7340032,"name":"Suresh Patil","title":"Rapid Detection of Highly Pathogenic Avian Influenza H5N1 Virus by TaqMan Reverse Transcriptase–Polymerase Chain Reaction"},{"id":7717027,"work_id":17119374,"tagging_user_id":36693995,"tagged_user_id":16665419,"co_author_invite_id":null,"email":"b***t@gmail.com","display_order":7864320,"name":"B.Sushant Patnaik","title":"Rapid Detection of Highly Pathogenic Avian Influenza H5N1 Virus by TaqMan Reverse Transcriptase–Polymerase Chain Reaction"},{"id":7717029,"work_id":17119374,"tagging_user_id":36693995,"tagged_user_id":null,"co_author_invite_id":1723460,"email":"d***p@gmail.com","display_order":8126464,"name":"Shankar B.p","title":"Rapid Detection of Highly Pathogenic Avian Influenza H5N1 Virus by TaqMan Reverse Transcriptase–Polymerase Chain Reaction"},{"id":7717030,"work_id":17119374,"tagging_user_id":36693995,"tagged_user_id":null,"co_author_invite_id":1723461,"email":"c***d@mit.asia","display_order":8257536,"name":"Patil S.s","title":"Rapid Detection of Highly Pathogenic Avian Influenza H5N1 Virus by TaqMan Reverse Transcriptase–Polymerase Chain Reaction"},{"id":7717100,"work_id":17119374,"tagging_user_id":36693995,"tagged_user_id":null,"co_author_invite_id":1723481,"email":"r***a@yahoo.co.in","display_order":8323072,"name":"Gowda R.n.s","title":"Rapid Detection of Highly Pathogenic Avian Influenza H5N1 Virus by TaqMan Reverse Transcriptase–Polymerase Chain Reaction"},{"id":7717101,"work_id":17119374,"tagging_user_id":36693995,"tagged_user_id":null,"co_author_invite_id":1723482,"email":"p***b@pdfmd.ernet.in","display_order":8355840,"name":"Pattnaik B","title":"Rapid Detection of Highly Pathogenic Avian Influenza H5N1 Virus by TaqMan Reverse Transcriptase–Polymerase Chain Reaction"}],"downloadable_attachments":[{"id":42316756,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/42316756/thumbnails/1.jpg","file_name":"fin1278.pdf","download_url":"https://www.academia.edu/attachments/42316756/download_file?st=MTczMzE5NzgyMSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Rapid_Detection_of_Highly_Pathogenic_Avi.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/42316756/fin1278-libre.pdf?1454868701=\u0026response-content-disposition=attachment%3B+filename%3DRapid_Detection_of_Highly_Pathogenic_Avi.pdf\u0026Expires=1733201421\u0026Signature=K8loWgGYBQzfjcZuKVVzfdAxfXc329cjeAODdoIl76Qm5juS~s1aS4UP~yxTz57GN8cRBGkp9KizsT~tSU-fKRJrbceZx1M5LrPovVz5qB0omlOZ7NrGmNIiqkn3y9iuJ~3TITjsPWOA72JSMwMtJcCF1O5WNeBodBvY-SMgzipTAXH2rD-MvstZSB7aqh260MRJkAjuGmixgfE95H69dm3WzPQYk~SUWfhcaV4olOBDkmeEOQiys9sbcnsZDa-RLdfMUsg-uCBbE~BIvQhwhFb9388cmVSwpjEZpfMCuD6atu-Xl3AYr2~Alujl3QjTyEra-TClcCycabcOu3~fFw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Rapid_Detection_of_Highly_Pathogenic_Avian_Influenza_H5N1_Virus_by_TaqMan_Reverse_Transcriptase_Polymerase_Chain_Reaction","translated_slug":"","page_count":4,"language":"en","content_type":"Work","owner":{"id":36693995,"first_name":"Hare","middle_initials":"","last_name":"Pradhan","page_name":"HarePradhan","domain_name":"independent","created_at":"2015-10-21T11:07:05.415-07:00","display_name":"Hare Pradhan","url":"https://independent.academia.edu/HarePradhan"},"attachments":[{"id":42316756,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/42316756/thumbnails/1.jpg","file_name":"fin1278.pdf","download_url":"https://www.academia.edu/attachments/42316756/download_file?st=MTczMzE5NzgyMSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Rapid_Detection_of_Highly_Pathogenic_Avi.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/42316756/fin1278-libre.pdf?1454868701=\u0026response-content-disposition=attachment%3B+filename%3DRapid_Detection_of_Highly_Pathogenic_Avi.pdf\u0026Expires=1733201421\u0026Signature=K8loWgGYBQzfjcZuKVVzfdAxfXc329cjeAODdoIl76Qm5juS~s1aS4UP~yxTz57GN8cRBGkp9KizsT~tSU-fKRJrbceZx1M5LrPovVz5qB0omlOZ7NrGmNIiqkn3y9iuJ~3TITjsPWOA72JSMwMtJcCF1O5WNeBodBvY-SMgzipTAXH2rD-MvstZSB7aqh260MRJkAjuGmixgfE95H69dm3WzPQYk~SUWfhcaV4olOBDkmeEOQiys9sbcnsZDa-RLdfMUsg-uCBbE~BIvQhwhFb9388cmVSwpjEZpfMCuD6atu-Xl3AYr2~Alujl3QjTyEra-TClcCycabcOu3~fFw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology"},{"id":173,"name":"Zoology","url":"https://www.academia.edu/Documents/in/Zoology"},{"id":3092,"name":"Poultry Science","url":"https://www.academia.edu/Documents/in/Poultry_Science"},{"id":51784,"name":"real time PCR","url":"https://www.academia.edu/Documents/in/real_time_PCR"},{"id":83205,"name":"Avian Influenza","url":"https://www.academia.edu/Documents/in/Avian_Influenza"},{"id":96324,"name":"Birds","url":"https://www.academia.edu/Documents/in/Birds"},{"id":238630,"name":"Experimental Infection","url":"https://www.academia.edu/Documents/in/Experimental_Infection"},{"id":371528,"name":"Early Detection","url":"https://www.academia.edu/Documents/in/Early_Detection"},{"id":644860,"name":"Veterinary Sciences","url":"https://www.academia.edu/Documents/in/Veterinary_Sciences"},{"id":770676,"name":"Avian Diseases","url":"https://www.academia.edu/Documents/in/Avian_Diseases"},{"id":2007837,"name":"Virus isolation","url":"https://www.academia.edu/Documents/in/Virus_isolation"},{"id":2029070,"name":"Highly pathogenic avian influenza","url":"https://www.academia.edu/Documents/in/Highly_pathogenic_avian_influenza"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="13998994"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/13998994/Genetic_typing_of_bovine_viral_diarrhoea_virus_isolates_from_India"><img alt="Research paper thumbnail of Genetic typing of bovine viral diarrhoea virus isolates from India" class="work-thumbnail" src="https://attachments.academia-assets.com/44717609/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/13998994/Genetic_typing_of_bovine_viral_diarrhoea_virus_isolates_from_India">Genetic typing of bovine viral diarrhoea virus isolates from India</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/StefanVilcek">Stefan Vilcek</a> and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/HarePradhan">Hare Pradhan</a></span></div><div class="wp-workCard_item"><span>Veterinary Microbiology</span><span>, 2004</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="75ba5e506c7bb5c83239aef01c67d768" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":44717609,"asset_id":13998994,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/44717609/download_file?st=MTczMzE5NzgyMSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="13998994"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="13998994"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 13998994; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=13998994]").text(description); $(".js-view-count[data-work-id=13998994]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 13998994; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='13998994']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 13998994, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "75ba5e506c7bb5c83239aef01c67d768" } } $('.js-work-strip[data-work-id=13998994]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":13998994,"title":"Genetic typing of bovine viral diarrhoea virus isolates from India","translated_title":"","metadata":{"ai_title_tag":"Genetic Typing of BVDV Isolates Across India","grobid_abstract":"Thirteen BVDV isolates collected in four geographic regions of India between 2000 and 2002 were typed in 5 0 -UTR. To confirm results of genetic typing, selected viruses were also analysed in the N pro region. Phylogenetic analysis revealed that all Indian BVDV isolates belong to BVDV-1b (Osloss-like group). Despite a long distance between the farms from which the viruses were isolated there was no correlation between the origin of viral isolates and their position in a phylogenetic tree. Higher genetic similarity of Indian BVDV isolates was observed most probably due to the uncontrolled movement of cattle as well as the uncontrolled use of semen from bulls for breeding of local and farm cattle in different states of India.","publication_date":{"day":null,"month":null,"year":2004,"errors":{}},"publication_name":"Veterinary Microbiology","grobid_abstract_attachment_id":44717609},"translated_abstract":null,"internal_url":"https://www.academia.edu/13998994/Genetic_typing_of_bovine_viral_diarrhoea_virus_isolates_from_India","translated_internal_url":"","created_at":"2015-07-13T10:43:02.263-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":33035758,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":3051774,"work_id":13998994,"tagging_user_id":33035758,"tagged_user_id":null,"co_author_invite_id":774789,"email":"d***l@gtnet.gov.uk","display_order":0,"name":"D. Paton","title":"Genetic typing of bovine viral diarrhoea virus isolates from India"},{"id":3051800,"work_id":13998994,"tagging_user_id":33035758,"tagged_user_id":57938591,"co_author_invite_id":774792,"email":"w***r@vu-wien.ac.at","display_order":4194304,"name":"W. Baumgartner","title":"Genetic typing of bovine viral diarrhoea virus isolates from India"},{"id":7483474,"work_id":13998994,"tagging_user_id":33035758,"tagged_user_id":null,"co_author_invite_id":1671833,"email":"d***c@vf.uni-lj.si","display_order":6291456,"name":"Darja Barlič-maganja","title":"Genetic typing of bovine viral diarrhoea virus isolates from India"},{"id":7483475,"work_id":13998994,"tagging_user_id":33035758,"tagged_user_id":36693995,"co_author_invite_id":1671834,"email":"h***5@rediffmail.com","display_order":7340032,"name":"Hare Pradhan","title":"Genetic typing of bovine viral diarrhoea virus isolates from India"},{"id":7483476,"work_id":13998994,"tagging_user_id":33035758,"tagged_user_id":20047756,"co_author_invite_id":null,"email":"j***4@gmail.com","display_order":7864320,"name":"Pooja Jain","title":"Genetic typing of bovine viral diarrhoea virus isolates from India"},{"id":7483477,"work_id":13998994,"tagging_user_id":33035758,"tagged_user_id":15560863,"co_author_invite_id":null,"email":"a***1@gmail.com","display_order":8126464,"name":"Pooja Jain","title":"Genetic typing of bovine viral diarrhoea virus isolates from India"},{"id":7483478,"work_id":13998994,"tagging_user_id":33035758,"tagged_user_id":18206113,"co_author_invite_id":null,"email":"j***3@gmail.com","display_order":8257536,"name":"Pooja Jain","title":"Genetic typing of bovine viral diarrhoea virus isolates from India"},{"id":7483479,"work_id":13998994,"tagging_user_id":33035758,"tagged_user_id":16219264,"co_author_invite_id":null,"email":"p***5@gmail.com","display_order":8323072,"name":"pooja jain","title":"Genetic typing of bovine viral diarrhoea virus isolates from India"},{"id":7483480,"work_id":13998994,"tagging_user_id":33035758,"tagged_user_id":12422335,"co_author_invite_id":null,"email":"p***n@gmail.com","display_order":8355840,"name":"pooja jain","title":"Genetic typing of bovine viral diarrhoea virus isolates from India"},{"id":7483481,"work_id":13998994,"tagging_user_id":33035758,"tagged_user_id":11839197,"co_author_invite_id":null,"email":"j***1@yahoo.co.in","display_order":8372224,"name":"POOJA JAIN","title":"Genetic typing of bovine viral diarrhoea virus isolates from India"},{"id":7483482,"work_id":13998994,"tagging_user_id":33035758,"tagged_user_id":null,"co_author_invite_id":750022,"email":"p***n@drexelmed.edu","display_order":8380416,"name":"Pooja Jain","title":"Genetic typing of bovine viral diarrhoea virus isolates from India"},{"id":7483483,"work_id":13998994,"tagging_user_id":33035758,"tagged_user_id":null,"co_author_invite_id":1671835,"email":"t***k@vla.defra.gsi.gov.uk","display_order":8384512,"name":"Torstein Sandvik","title":"Genetic typing of bovine viral diarrhoea virus isolates from India"}],"downloadable_attachments":[{"id":44717609,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/44717609/thumbnails/1.jpg","file_name":"0deec5297319827dcf000000.pdf","download_url":"https://www.academia.edu/attachments/44717609/download_file?st=MTczMzE5NzgyMSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Genetic_typing_of_bovine_viral_diarrhoea.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/44717609/0deec5297319827dcf000000-libre.pdf?1460615205=\u0026response-content-disposition=attachment%3B+filename%3DGenetic_typing_of_bovine_viral_diarrhoea.pdf\u0026Expires=1733201421\u0026Signature=GMJ1TI4gDUD1o7oMGBKsfceRSX5CN3Ehur2-8U1Y1KffbYtxWePw9eG-WGdiZEkeYnIfxTmzc5AgiBxCWM0h0qeP7F5TJQvC6~hlalk6CJa6C8g4P-lZl6qmwgIJekhMhAiDbZFda5s0pvNW7-A0gA4FafRDWgQkXU5EeJDt5z2BLB3KWCiwZDmXXMDCdiqk7IqqkYY-haaB~XdD4fPxkPHe8luZFoGgYJU5I~L25xk6vlh8n4OLbwzv56uriFKmRkZ8wtQIB1z8f6j2I4QPeGwE6gEK~RJTB9Pm9LeU1Kh6nfh9ktrUmpWZtAeKzGWPpPiGgm2JAKCZxGUKN3s2nA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Genetic_typing_of_bovine_viral_diarrhoea_virus_isolates_from_India","translated_slug":"","page_count":6,"language":"en","content_type":"Work","owner":{"id":33035758,"first_name":"Stefan","middle_initials":null,"last_name":"Vilcek","page_name":"StefanVilcek","domain_name":"independent","created_at":"2015-07-13T10:42:22.347-07:00","display_name":"Stefan Vilcek","url":"https://independent.academia.edu/StefanVilcek"},"attachments":[{"id":44717609,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/44717609/thumbnails/1.jpg","file_name":"0deec5297319827dcf000000.pdf","download_url":"https://www.academia.edu/attachments/44717609/download_file?st=MTczMzE5NzgyMSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Genetic_typing_of_bovine_viral_diarrhoea.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/44717609/0deec5297319827dcf000000-libre.pdf?1460615205=\u0026response-content-disposition=attachment%3B+filename%3DGenetic_typing_of_bovine_viral_diarrhoea.pdf\u0026Expires=1733201421\u0026Signature=GMJ1TI4gDUD1o7oMGBKsfceRSX5CN3Ehur2-8U1Y1KffbYtxWePw9eG-WGdiZEkeYnIfxTmzc5AgiBxCWM0h0qeP7F5TJQvC6~hlalk6CJa6C8g4P-lZl6qmwgIJekhMhAiDbZFda5s0pvNW7-A0gA4FafRDWgQkXU5EeJDt5z2BLB3KWCiwZDmXXMDCdiqk7IqqkYY-haaB~XdD4fPxkPHe8luZFoGgYJU5I~L25xk6vlh8n4OLbwzv56uriFKmRkZ8wtQIB1z8f6j2I4QPeGwE6gEK~RJTB9Pm9LeU1Kh6nfh9ktrUmpWZtAeKzGWPpPiGgm2JAKCZxGUKN3s2nA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":156,"name":"Genetics","url":"https://www.academia.edu/Documents/in/Genetics"},{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology"},{"id":3037,"name":"Veterinary Microbiology","url":"https://www.academia.edu/Documents/in/Veterinary_Microbiology"},{"id":35414,"name":"Veterinary","url":"https://www.academia.edu/Documents/in/Veterinary"},{"id":37782,"name":"Cell Culture","url":"https://www.academia.edu/Documents/in/Cell_Culture"},{"id":43838,"name":"India","url":"https://www.academia.edu/Documents/in/India"},{"id":54433,"name":"Phylogeny","url":"https://www.academia.edu/Documents/in/Phylogeny"},{"id":67484,"name":"Sequence alignment","url":"https://www.academia.edu/Documents/in/Sequence_alignment"},{"id":158782,"name":"Phylogenetic analysis","url":"https://www.academia.edu/Documents/in/Phylogenetic_analysis"},{"id":178709,"name":"Slovenia","url":"https://www.academia.edu/Documents/in/Slovenia"},{"id":260829,"name":"Cattle","url":"https://www.academia.edu/Documents/in/Cattle"},{"id":372410,"name":"Genotype","url":"https://www.academia.edu/Documents/in/Genotype"},{"id":486784,"name":"Genotyping","url":"https://www.academia.edu/Documents/in/Genotyping"},{"id":550697,"name":"Phylogenetic Tree","url":"https://www.academia.edu/Documents/in/Phylogenetic_Tree"},{"id":577933,"name":"Genetic variation","url":"https://www.academia.edu/Documents/in/Genetic_variation"},{"id":644860,"name":"Veterinary Sciences","url":"https://www.academia.edu/Documents/in/Veterinary_Sciences"},{"id":809882,"name":"Base Sequence","url":"https://www.academia.edu/Documents/in/Base_Sequence"},{"id":964206,"name":"Genetic Similarity","url":"https://www.academia.edu/Documents/in/Genetic_Similarity"},{"id":1714028,"name":"Long Distance","url":"https://www.academia.edu/Documents/in/Long_Distance"},{"id":2007837,"name":"Virus isolation","url":"https://www.academia.edu/Documents/in/Virus_isolation"},{"id":2056385,"name":"Bovine viral diarrhoea","url":"https://www.academia.edu/Documents/in/Bovine_viral_diarrhoea"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> </div><div class="profile--tab_content_container js-tab-pane tab-pane" data-section-id="3809269" id="papers"><div class="js-work-strip profile--work_container" data-work-id="17119392"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/17119392/Dandapat_S_Pradhan_H_K_and_Mohanty_G_C_1994_Anti_idiotype_antibodies_to_Marek_s_disease_associated_tumor_surface_antigens_in_protection_against_Marek_s_disease_Vet_Immunol_Immunopathol_40_353_366"><img alt="Research paper thumbnail of Dandapat, S., Pradhan, H.K. and Mohanty, G.C. (1994). Anti-idiotype antibodies to Marek’s disease – associated tumor surface antigens in protection against Marek’s disease. Vet. Immunol. Immunopathol., 40: 353-366" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/17119392/Dandapat_S_Pradhan_H_K_and_Mohanty_G_C_1994_Anti_idiotype_antibodies_to_Marek_s_disease_associated_tumor_surface_antigens_in_protection_against_Marek_s_disease_Vet_Immunol_Immunopathol_40_353_366">Dandapat, S., Pradhan, H.K. and Mohanty, G.C. (1994). Anti-idiotype antibodies to Marek’s disease – associated tumor surface antigens in protection against Marek’s disease. Vet. Immunol. Immunopathol., 40: 353-366</a></div><div class="wp-workCard_item"><span>Veterinary Immunology and Immunopathology</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="17119392"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="17119392"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 17119392; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=17119392]").text(description); $(".js-view-count[data-work-id=17119392]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 17119392; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='17119392']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 17119392, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=17119392]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":17119392,"title":"Dandapat, S., Pradhan, H.K. and Mohanty, G.C. (1994). Anti-idiotype antibodies to Marek’s disease – associated tumor surface antigens in protection against Marek’s disease. Vet. Immunol. Immunopathol., 40: 353-366","translated_title":"","metadata":{"publication_name":"Veterinary Immunology and Immunopathology"},"translated_abstract":null,"internal_url":"https://www.academia.edu/17119392/Dandapat_S_Pradhan_H_K_and_Mohanty_G_C_1994_Anti_idiotype_antibodies_to_Marek_s_disease_associated_tumor_surface_antigens_in_protection_against_Marek_s_disease_Vet_Immunol_Immunopathol_40_353_366","translated_internal_url":"","created_at":"2015-10-21T11:12:55.511-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":36693995,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":7717087,"work_id":17119392,"tagging_user_id":36693995,"tagged_user_id":12271862,"co_author_invite_id":null,"email":"g***y@gmail.com","display_order":0,"name":"Gagan Mohanty","title":"Dandapat, S., Pradhan, H.K. and Mohanty, G.C. (1994). Anti-idiotype antibodies to Marek’s disease – associated tumor surface antigens in protection against Marek’s disease. Vet. Immunol. Immunopathol., 40: 353-366"},{"id":7717092,"work_id":17119392,"tagging_user_id":36693995,"tagged_user_id":null,"co_author_invite_id":1723478,"email":"s***t@hotmail.com","display_order":4194304,"name":"S. Dandapat","title":"Dandapat, S., Pradhan, H.K. and Mohanty, G.C. (1994). Anti-idiotype antibodies to Marek’s disease – associated tumor surface antigens in protection against Marek’s disease. Vet. Immunol. Immunopathol., 40: 353-366"}],"downloadable_attachments":[],"slug":"Dandapat_S_Pradhan_H_K_and_Mohanty_G_C_1994_Anti_idiotype_antibodies_to_Marek_s_disease_associated_tumor_surface_antigens_in_protection_against_Marek_s_disease_Vet_Immunol_Immunopathol_40_353_366","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":36693995,"first_name":"Hare","middle_initials":"","last_name":"Pradhan","page_name":"HarePradhan","domain_name":"independent","created_at":"2015-10-21T11:07:05.415-07:00","display_name":"Hare Pradhan","url":"https://independent.academia.edu/HarePradhan"},"attachments":[],"research_interests":[{"id":173,"name":"Zoology","url":"https://www.academia.edu/Documents/in/Zoology"},{"id":644860,"name":"Veterinary Sciences","url":"https://www.academia.edu/Documents/in/Veterinary_Sciences"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="17119391"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/17119391/Genetic_analysis_of_indian_bovine_viral_diarrhea_virus_1_isolates_in_N_pro_and_entire_gene_region_coding_structural_proteins"><img alt="Research paper thumbnail of Genetic analysis of indian bovine viral diarrhea virus 1 isolates in N(pro) and entire gene region coding structural proteins" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/17119391/Genetic_analysis_of_indian_bovine_viral_diarrhea_virus_1_isolates_in_N_pro_and_entire_gene_region_coding_structural_proteins">Genetic analysis of indian bovine viral diarrhea virus 1 isolates in N(pro) and entire gene region coding structural proteins</a></div><div class="wp-workCard_item"><span>Acta virologica</span><span>, 2006</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Three Indian Bovine viral diarrhea virus 1 (BVDV-1) isolates were analyzed at genetic level in N(...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Three Indian Bovine viral diarrhea virus 1 (BVDV-1) isolates were analyzed at genetic level in N(pro) (viral autoprotease) and entire gene region coding structural proteins, namely capsid (C) protein, E(rns), and envelope proteins E1 and E2. All these isolates were found to be of b subtype based on the entire 504 nt region of N(pro) and 1119 nt region of E2. However, in comparison with other isolates of this subtype, they were allocated inside the BVDV-1 subtype b cluster to a separate clade with a longer distance. Of six cysteine residues in N(pro) only three were totally conserved in all three isolates. The isolates showed 94.9-99.3% and 92.2-99.0% identities for the entire C-E2 gene region at nucleotide and amino acid levels, respectively. The lowest identity values (88.5-91.7%) were observed for E2 amino acid sequences. The identity of the isolates with Osloss, a reference BVDV-1 subtype b strain, was in the range of 82.1-89.9% for nucleotide and 78.6-89.2% for amino acid sequen...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="17119391"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="17119391"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 17119391; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=17119391]").text(description); $(".js-view-count[data-work-id=17119391]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 17119391; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='17119391']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 17119391, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=17119391]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":17119391,"title":"Genetic analysis of indian bovine viral diarrhea virus 1 isolates in N(pro) and entire gene region coding structural proteins","translated_title":"","metadata":{"abstract":"Three Indian Bovine viral diarrhea virus 1 (BVDV-1) isolates were analyzed at genetic level in N(pro) (viral autoprotease) and entire gene region coding structural proteins, namely capsid (C) protein, E(rns), and envelope proteins E1 and E2. All these isolates were found to be of b subtype based on the entire 504 nt region of N(pro) and 1119 nt region of E2. However, in comparison with other isolates of this subtype, they were allocated inside the BVDV-1 subtype b cluster to a separate clade with a longer distance. Of six cysteine residues in N(pro) only three were totally conserved in all three isolates. The isolates showed 94.9-99.3% and 92.2-99.0% identities for the entire C-E2 gene region at nucleotide and amino acid levels, respectively. The lowest identity values (88.5-91.7%) were observed for E2 amino acid sequences. The identity of the isolates with Osloss, a reference BVDV-1 subtype b strain, was in the range of 82.1-89.9% for nucleotide and 78.6-89.2% for amino acid sequen...","publication_date":{"day":null,"month":null,"year":2006,"errors":{}},"publication_name":"Acta virologica"},"translated_abstract":"Three Indian Bovine viral diarrhea virus 1 (BVDV-1) isolates were analyzed at genetic level in N(pro) (viral autoprotease) and entire gene region coding structural proteins, namely capsid (C) protein, E(rns), and envelope proteins E1 and E2. All these isolates were found to be of b subtype based on the entire 504 nt region of N(pro) and 1119 nt region of E2. However, in comparison with other isolates of this subtype, they were allocated inside the BVDV-1 subtype b cluster to a separate clade with a longer distance. Of six cysteine residues in N(pro) only three were totally conserved in all three isolates. The isolates showed 94.9-99.3% and 92.2-99.0% identities for the entire C-E2 gene region at nucleotide and amino acid levels, respectively. The lowest identity values (88.5-91.7%) were observed for E2 amino acid sequences. The identity of the isolates with Osloss, a reference BVDV-1 subtype b strain, was in the range of 82.1-89.9% for nucleotide and 78.6-89.2% for amino acid sequen...","internal_url":"https://www.academia.edu/17119391/Genetic_analysis_of_indian_bovine_viral_diarrhea_virus_1_isolates_in_N_pro_and_entire_gene_region_coding_structural_proteins","translated_internal_url":"","created_at":"2015-10-21T11:12:55.425-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":36693995,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":7717033,"work_id":17119391,"tagging_user_id":36693995,"tagged_user_id":50823026,"co_author_invite_id":616470,"email":"m***r@rediffmail.com","display_order":0,"name":"Niranjan Mishra","title":"Genetic analysis of indian bovine viral diarrhea virus 1 isolates in N(pro) and entire gene region coding structural proteins"},{"id":7717037,"work_id":17119391,"tagging_user_id":36693995,"tagged_user_id":1175172,"co_author_invite_id":null,"email":"s***9@gmail.com","display_order":4194304,"name":"Shreyas Pitale","title":"Genetic analysis of indian bovine viral diarrhea virus 1 isolates in N(pro) and entire gene region coding structural proteins"},{"id":7717095,"work_id":17119391,"tagging_user_id":36693995,"tagged_user_id":8516907,"co_author_invite_id":null,"email":"p***n@ignou.ac.in","display_order":6291456,"name":"PRAVEEN KUMAR JAIN","title":"Genetic analysis of indian bovine viral diarrhea virus 1 isolates in N(pro) and entire gene region coding structural proteins"},{"id":7717099,"work_id":17119391,"tagging_user_id":36693995,"tagged_user_id":33035758,"co_author_invite_id":null,"email":"v***k@uvm.sk","display_order":7340032,"name":"Stefan Vilcek","title":"Genetic analysis of indian bovine viral diarrhea virus 1 isolates in N(pro) and entire gene region coding structural proteins"}],"downloadable_attachments":[],"slug":"Genetic_analysis_of_indian_bovine_viral_diarrhea_virus_1_isolates_in_N_pro_and_entire_gene_region_coding_structural_proteins","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":36693995,"first_name":"Hare","middle_initials":"","last_name":"Pradhan","page_name":"HarePradhan","domain_name":"independent","created_at":"2015-10-21T11:07:05.415-07:00","display_name":"Hare Pradhan","url":"https://independent.academia.edu/HarePradhan"},"attachments":[],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology"},{"id":6947,"name":"Medical Microbiology","url":"https://www.academia.edu/Documents/in/Medical_Microbiology"},{"id":74780,"name":"Mutation","url":"https://www.academia.edu/Documents/in/Mutation"},{"id":260829,"name":"Cattle","url":"https://www.academia.edu/Documents/in/Cattle"},{"id":809881,"name":"Amino Acid Sequence","url":"https://www.academia.edu/Documents/in/Amino_Acid_Sequence"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="17119390"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/17119390/Bioterrorism_How_prepared_are_we"><img alt="Research paper thumbnail of Bioterrorism: How prepared are we?" class="work-thumbnail" src="https://attachments.academia-assets.com/42316763/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/17119390/Bioterrorism_How_prepared_are_we">Bioterrorism: How prepared are we?</a></div><div class="wp-workCard_item"><span>Indian Journal of Medical Specialities</span><span>, 2012</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="d46fbf84982c97a5e6991983db389909" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":42316763,"asset_id":17119390,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/42316763/download_file?st=MTczMzE5NzgyMSw4LjIyMi4yMDguMTQ2&st=MTczMzE5NzgyMSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="17119390"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="17119390"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 17119390; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=17119390]").text(description); $(".js-view-count[data-work-id=17119390]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 17119390; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='17119390']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 17119390, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "d46fbf84982c97a5e6991983db389909" } } $('.js-work-strip[data-work-id=17119390]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":17119390,"title":"Bioterrorism: How prepared are we?","translated_title":"","metadata":{"publication_date":{"day":null,"month":null,"year":2012,"errors":{}},"publication_name":"Indian Journal of Medical Specialities"},"translated_abstract":null,"internal_url":"https://www.academia.edu/17119390/Bioterrorism_How_prepared_are_we","translated_internal_url":"","created_at":"2015-10-21T11:12:55.333-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":36693995,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":7717022,"work_id":17119390,"tagging_user_id":36693995,"tagged_user_id":null,"co_author_invite_id":124581,"email":"p***b@gmail.com","display_order":0,"name":"B. Pattnaik","title":"Bioterrorism: How prepared are we?"},{"id":7717096,"work_id":17119390,"tagging_user_id":36693995,"tagged_user_id":319840673,"co_author_invite_id":320366,"email":"d***r@gmail.com","display_order":4194304,"name":"Sitanshu Kar","title":"Bioterrorism: How prepared are we?"},{"id":7717097,"work_id":17119390,"tagging_user_id":36693995,"tagged_user_id":null,"co_author_invite_id":1723480,"email":"d***a@miami.edu","display_order":6291456,"name":"D. Shalala","title":"Bioterrorism: How prepared are we?"}],"downloadable_attachments":[{"id":42316763,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/42316763/thumbnails/1.jpg","file_name":"Bioterrorism_How_prepared_are_we20160207-11024-1s8pyac.pdf","download_url":"https://www.academia.edu/attachments/42316763/download_file?st=MTczMzE5NzgyMSw4LjIyMi4yMDguMTQ2&st=MTczMzE5NzgyMSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Bioterrorism_How_prepared_are_we.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/42316763/Bioterrorism_How_prepared_are_we20160207-11024-1s8pyac-libre.pdf?1454868701=\u0026response-content-disposition=attachment%3B+filename%3DBioterrorism_How_prepared_are_we.pdf\u0026Expires=1733201421\u0026Signature=YIXy-d3Zfoo9pqL8t~iwvHH1Y2pAkhisovdR9-JvfIqWP3foH~VzJyDKVjGy3WwuoG1v4GdsTTBNq3ZJQhxtk9ny36anzdggG3wB5-askOaVLn8cHxVmiFJACwTrTdYaQsJlisWHAdRdArV8TKGo0q7fWxBHXsg8DhNjDAO7RaxW9AZ2lb~wb7~O4wZEbs6oSueBWvGLuBgAb47bdXnMC9A6cqrFpM47ZCSC1Fxrzzfgl2Ym80FP0IotR-UmdR6y6u7FWNDhhjYAnnF5Zk4sGJwb1qlzGjIceEuois2UhHXgMVzLAr6VMEtBeI2MCoNsVUk4cUP7SqOD2KYKuNN7Gw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Bioterrorism_How_prepared_are_we","translated_slug":"","page_count":6,"language":"en","content_type":"Work","owner":{"id":36693995,"first_name":"Hare","middle_initials":"","last_name":"Pradhan","page_name":"HarePradhan","domain_name":"independent","created_at":"2015-10-21T11:07:05.415-07:00","display_name":"Hare Pradhan","url":"https://independent.academia.edu/HarePradhan"},"attachments":[{"id":42316763,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/42316763/thumbnails/1.jpg","file_name":"Bioterrorism_How_prepared_are_we20160207-11024-1s8pyac.pdf","download_url":"https://www.academia.edu/attachments/42316763/download_file?st=MTczMzE5NzgyMSw4LjIyMi4yMDguMTQ2&st=MTczMzE5NzgyMSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Bioterrorism_How_prepared_are_we.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/42316763/Bioterrorism_How_prepared_are_we20160207-11024-1s8pyac-libre.pdf?1454868701=\u0026response-content-disposition=attachment%3B+filename%3DBioterrorism_How_prepared_are_we.pdf\u0026Expires=1733201421\u0026Signature=YIXy-d3Zfoo9pqL8t~iwvHH1Y2pAkhisovdR9-JvfIqWP3foH~VzJyDKVjGy3WwuoG1v4GdsTTBNq3ZJQhxtk9ny36anzdggG3wB5-askOaVLn8cHxVmiFJACwTrTdYaQsJlisWHAdRdArV8TKGo0q7fWxBHXsg8DhNjDAO7RaxW9AZ2lb~wb7~O4wZEbs6oSueBWvGLuBgAb47bdXnMC9A6cqrFpM47ZCSC1Fxrzzfgl2Ym80FP0IotR-UmdR6y6u7FWNDhhjYAnnF5Zk4sGJwb1qlzGjIceEuois2UhHXgMVzLAr6VMEtBeI2MCoNsVUk4cUP7SqOD2KYKuNN7Gw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":1697,"name":"Violence","url":"https://www.academia.edu/Documents/in/Violence"},{"id":6947,"name":"Medical Microbiology","url":"https://www.academia.edu/Documents/in/Medical_Microbiology"},{"id":16288,"name":"Public Health","url":"https://www.academia.edu/Documents/in/Public_Health"},{"id":17969,"name":"Health Policy","url":"https://www.academia.edu/Documents/in/Health_Policy"},{"id":40574,"name":"Emerging Infectious Diseases","url":"https://www.academia.edu/Documents/in/Emerging_Infectious_Diseases"},{"id":98134,"name":"United States","url":"https://www.academia.edu/Documents/in/United_States"},{"id":244814,"name":"Clinical Sciences","url":"https://www.academia.edu/Documents/in/Clinical_Sciences"},{"id":332907,"name":"Biological Warfare","url":"https://www.academia.edu/Documents/in/Biological_Warfare"},{"id":410370,"name":"Public health systems and services research","url":"https://www.academia.edu/Documents/in/Public_health_systems_and_services_research-1"}],"urls":[{"id":6522411,"url":"https://www.researchgate.net/profile/Sitanshu_Kar/publication/272717872_Bioterrorism_How_prepared_are_we/links/54f69f1c0cf21d8b8a5cfb6d.pdf"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="17119389"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/17119389/Histamine_in_Foods_Its_Safety_and_Human_Health_Implications"><img alt="Research paper thumbnail of Histamine in Foods: Its Safety and Human Health Implications" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/17119389/Histamine_in_Foods_Its_Safety_and_Human_Health_Implications">Histamine in Foods: Its Safety and Human Health Implications</a></div><div class="wp-workCard_item"><span>Journal of Food Science and Technology Nepal</span><span>, 2014</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="17119389"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="17119389"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 17119389; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=17119389]").text(description); $(".js-view-count[data-work-id=17119389]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 17119389; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='17119389']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 17119389, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=17119389]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":17119389,"title":"Histamine in Foods: Its Safety and Human Health Implications","translated_title":"","metadata":{"publication_date":{"day":null,"month":null,"year":2014,"errors":{}},"publication_name":"Journal of Food Science and Technology Nepal"},"translated_abstract":null,"internal_url":"https://www.academia.edu/17119389/Histamine_in_Foods_Its_Safety_and_Human_Health_Implications","translated_internal_url":"","created_at":"2015-10-21T11:12:55.253-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":36693995,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":7717102,"work_id":17119389,"tagging_user_id":36693995,"tagged_user_id":4842777,"co_author_invite_id":null,"email":"r***6@gmail.com","display_order":0,"name":"Krishna Rai","title":"Histamine in Foods: Its Safety and Human Health Implications"}],"downloadable_attachments":[],"slug":"Histamine_in_Foods_Its_Safety_and_Human_Health_Implications","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":36693995,"first_name":"Hare","middle_initials":"","last_name":"Pradhan","page_name":"HarePradhan","domain_name":"independent","created_at":"2015-10-21T11:07:05.415-07:00","display_name":"Hare Pradhan","url":"https://independent.academia.edu/HarePradhan"},"attachments":[],"research_interests":[{"id":49161,"name":"Safety","url":"https://www.academia.edu/Documents/in/Safety"},{"id":51569,"name":"Histamine","url":"https://www.academia.edu/Documents/in/Histamine"},{"id":65881,"name":"FISH","url":"https://www.academia.edu/Documents/in/FISH"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="17119388"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/17119388/Genetic_and_antigenic_characterization_of_bovine_viral_diarrhea_virus_type_2_isolated_from_Indian_goats_Capra_hircus_"><img alt="Research paper thumbnail of Genetic and antigenic characterization of bovine viral diarrhea virus type 2 isolated from Indian goats (Capra hircus)" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/17119388/Genetic_and_antigenic_characterization_of_bovine_viral_diarrhea_virus_type_2_isolated_from_Indian_goats_Capra_hircus_">Genetic and antigenic characterization of bovine viral diarrhea virus type 2 isolated from Indian goats (Capra hircus)</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/HarePradhan">Hare Pradhan</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/ATiwari1">A. Tiwari</a>, and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/ChakradharTosh">Chakradhar Tosh</a></span></div><div class="wp-workCard_item"><span>Veterinary Microbiology</span><span>, 2007</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Recent studies have shown that bovine viral diarrhea virus (BVDV) type 1 is widely prevalent in I...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Recent studies have shown that bovine viral diarrhea virus (BVDV) type 1 is widely prevalent in Indian cattle. In a surveillance of randomly collected 562 blood samples from seven states during 2004-2006, BVDV type 2 was detected in two native Indian goats by nested reverse transcription polymerase chain reaction (nRT-PCR). The virus isolated from them was classified antigenically as BVDV 2 on the basis of virus neutralization test and reactivity with monoclonal antibodies. Phylogenetic analysis of three different genomic regions, 5&amp;#39; un-translated region (5&amp;#39; UTR), E(rns) structural coding region and NS5B nonstructural coding region typed Indian goat isolate as BVDV 2a having close similarity with strains from North America and Europe suggesting its probable introduction through trade. It was placed in a separate clade within the 2a branch having unique mutations in E(rns) and NS5B region. This is the first report of BVDV 2 in India and only second time recorded in goat species. The isolation of BVDV 2 from goat warrants intensive surveillance in cattle and sheep.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="17119388"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="17119388"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 17119388; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=17119388]").text(description); $(".js-view-count[data-work-id=17119388]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 17119388; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='17119388']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 17119388, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=17119388]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":17119388,"title":"Genetic and antigenic characterization of bovine viral diarrhea virus type 2 isolated from Indian goats (Capra hircus)","translated_title":"","metadata":{"abstract":"Recent studies have shown that bovine viral diarrhea virus (BVDV) type 1 is widely prevalent in Indian cattle. In a surveillance of randomly collected 562 blood samples from seven states during 2004-2006, BVDV type 2 was detected in two native Indian goats by nested reverse transcription polymerase chain reaction (nRT-PCR). The virus isolated from them was classified antigenically as BVDV 2 on the basis of virus neutralization test and reactivity with monoclonal antibodies. Phylogenetic analysis of three different genomic regions, 5\u0026amp;#39; un-translated region (5\u0026amp;#39; UTR), E(rns) structural coding region and NS5B nonstructural coding region typed Indian goat isolate as BVDV 2a having close similarity with strains from North America and Europe suggesting its probable introduction through trade. It was placed in a separate clade within the 2a branch having unique mutations in E(rns) and NS5B region. This is the first report of BVDV 2 in India and only second time recorded in goat species. The isolation of BVDV 2 from goat warrants intensive surveillance in cattle and sheep.","publication_date":{"day":null,"month":null,"year":2007,"errors":{}},"publication_name":"Veterinary Microbiology"},"translated_abstract":"Recent studies have shown that bovine viral diarrhea virus (BVDV) type 1 is widely prevalent in Indian cattle. In a surveillance of randomly collected 562 blood samples from seven states during 2004-2006, BVDV type 2 was detected in two native Indian goats by nested reverse transcription polymerase chain reaction (nRT-PCR). The virus isolated from them was classified antigenically as BVDV 2 on the basis of virus neutralization test and reactivity with monoclonal antibodies. Phylogenetic analysis of three different genomic regions, 5\u0026amp;#39; un-translated region (5\u0026amp;#39; UTR), E(rns) structural coding region and NS5B nonstructural coding region typed Indian goat isolate as BVDV 2a having close similarity with strains from North America and Europe suggesting its probable introduction through trade. It was placed in a separate clade within the 2a branch having unique mutations in E(rns) and NS5B region. This is the first report of BVDV 2 in India and only second time recorded in goat species. The isolation of BVDV 2 from goat warrants intensive surveillance in cattle and sheep.","internal_url":"https://www.academia.edu/17119388/Genetic_and_antigenic_characterization_of_bovine_viral_diarrhea_virus_type_2_isolated_from_Indian_goats_Capra_hircus_","translated_internal_url":"","created_at":"2015-10-21T11:12:55.157-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":36693995,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":7717034,"work_id":17119388,"tagging_user_id":36693995,"tagged_user_id":50823026,"co_author_invite_id":616470,"email":"m***r@rediffmail.com","display_order":0,"name":"Niranjan Mishra","title":"Genetic and antigenic characterization of bovine viral diarrhea virus type 2 isolated from Indian goats (Capra hircus)"},{"id":7717038,"work_id":17119388,"tagging_user_id":36693995,"tagged_user_id":1175172,"co_author_invite_id":null,"email":"s***9@gmail.com","display_order":4194304,"name":"Shreyas Pitale","title":"Genetic and antigenic characterization of bovine viral diarrhea virus type 2 isolated from Indian goats (Capra hircus)"},{"id":7717063,"work_id":17119388,"tagging_user_id":36693995,"tagged_user_id":null,"co_author_invite_id":1723468,"email":"k***r@rediffmail.com","display_order":6291456,"name":"K. Rajukumar","title":"Genetic and antigenic characterization of bovine viral diarrhea virus type 2 isolated from Indian goats (Capra hircus)"},{"id":7717066,"work_id":17119388,"tagging_user_id":36693995,"tagged_user_id":null,"co_author_invite_id":1723470,"email":"r***u@rediffmail.com","display_order":7340032,"name":"R. Dubey","title":"Genetic and antigenic characterization of bovine viral diarrhea virus type 2 isolated from Indian goats (Capra hircus)"},{"id":7717068,"work_id":17119388,"tagging_user_id":36693995,"tagged_user_id":36958264,"co_author_invite_id":1723471,"email":"a***3@yahoo.com","display_order":7864320,"name":"A. Tiwari","title":"Genetic and antigenic characterization of bovine viral diarrhea virus type 2 isolated from Indian goats (Capra hircus)"},{"id":7717094,"work_id":17119388,"tagging_user_id":36693995,"tagged_user_id":50718669,"co_author_invite_id":522492,"email":"c***h@gmail.com","display_order":8126464,"name":"Chakradhar Tosh","title":"Genetic and antigenic characterization of bovine viral diarrhea virus type 2 isolated from Indian goats (Capra hircus)"}],"downloadable_attachments":[],"slug":"Genetic_and_antigenic_characterization_of_bovine_viral_diarrhea_virus_type_2_isolated_from_Indian_goats_Capra_hircus_","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":36693995,"first_name":"Hare","middle_initials":"","last_name":"Pradhan","page_name":"HarePradhan","domain_name":"independent","created_at":"2015-10-21T11:07:05.415-07:00","display_name":"Hare Pradhan","url":"https://independent.academia.edu/HarePradhan"},"attachments":[],"research_interests":[{"id":156,"name":"Genetics","url":"https://www.academia.edu/Documents/in/Genetics"},{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology"},{"id":3037,"name":"Veterinary Microbiology","url":"https://www.academia.edu/Documents/in/Veterinary_Microbiology"},{"id":35414,"name":"Veterinary","url":"https://www.academia.edu/Documents/in/Veterinary"},{"id":43838,"name":"India","url":"https://www.academia.edu/Documents/in/India"},{"id":54433,"name":"Phylogeny","url":"https://www.academia.edu/Documents/in/Phylogeny"},{"id":103298,"name":"Blood sampling","url":"https://www.academia.edu/Documents/in/Blood_sampling"},{"id":147196,"name":"Monoclonal Antibodies","url":"https://www.academia.edu/Documents/in/Monoclonal_Antibodies"},{"id":158782,"name":"Phylogenetic analysis","url":"https://www.academia.edu/Documents/in/Phylogenetic_analysis"},{"id":252969,"name":"Goats","url":"https://www.academia.edu/Documents/in/Goats"},{"id":253469,"name":"North America","url":"https://www.academia.edu/Documents/in/North_America"},{"id":644860,"name":"Veterinary Sciences","url":"https://www.academia.edu/Documents/in/Veterinary_Sciences"},{"id":766014,"name":"Monoclonal Antibody","url":"https://www.academia.edu/Documents/in/Monoclonal_Antibody"},{"id":809881,"name":"Amino Acid Sequence","url":"https://www.academia.edu/Documents/in/Amino_Acid_Sequence"},{"id":1126122,"name":"Capra Hircus","url":"https://www.academia.edu/Documents/in/Capra_Hircus"},{"id":1709309,"name":"Bovine Viral Diarrhea Virus","url":"https://www.academia.edu/Documents/in/Bovine_Viral_Diarrhea_Virus"},{"id":2007837,"name":"Virus isolation","url":"https://www.academia.edu/Documents/in/Virus_isolation"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="17119387"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/17119387/Anti_idiotype_antibodies_to_Mareks_disease_associated_tumour_surface_antigen_in_protection_against_Mareks_disease"><img alt="Research paper thumbnail of Anti-idiotype antibodies to Marek's disease-associated tumour surface antigen in protection against Marek's disease" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/17119387/Anti_idiotype_antibodies_to_Mareks_disease_associated_tumour_surface_antigen_in_protection_against_Mareks_disease">Anti-idiotype antibodies to Marek's disease-associated tumour surface antigen in protection against Marek's disease</a></div><div class="wp-workCard_item"><span>Veterinary Immunology and Immunopathology</span><span>, 1994</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Marek&amp;#39;s disease-associated tumour surface antigen (MATSA) removed by enzymatic (papain) d...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Marek&amp;#39;s disease-associated tumour surface antigen (MATSA) removed by enzymatic (papain) digestion of Marek&amp;#39;s disease tumour cells was fractionated by gel filtration chromatography. The first peak (F1) was used to raise antibody in rabbits. Monoclonal antibody (RPH-6) directed against MATSA and the anti-F1 IgG were used as idiotypic antibodies to raise polyclonal anti-idiotype serum in heterologous hosts; rabbit and goat, respectively. The anti-idiotypes (anti-Id) were purified by affinity chromatography and characterized by competitive binding assay using immunofluorescent (IF) tests. Day-old white Leghorn chicks were immunized with anti-Id to MATSA (Group 1) or anti-Id to F1 (Group 3) and challenged with virulent Marek&amp;#39;s disease virus (MDV) on the tenth day post immunization. In positive control groups, the day-old chicks were inoculated with anti-BALB/c mouse globulin (Group 2) and anti-rabbit globulin (Group 4) and challenged with virulent MDV on the tenth day post inoculation. As compared with positive control groups, the vaccinated groups (1 and 3) had considerably lower level of MATSA positive cells during the post challenge observation period. The protection level against MD in the immunized groups was 66.6% (Group 1) and 86.6% (Group 3).</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="17119387"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="17119387"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 17119387; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=17119387]").text(description); $(".js-view-count[data-work-id=17119387]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 17119387; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='17119387']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 17119387, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=17119387]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":17119387,"title":"Anti-idiotype antibodies to Marek's disease-associated tumour surface antigen in protection against Marek's disease","translated_title":"","metadata":{"abstract":"Marek\u0026amp;#39;s disease-associated tumour surface antigen (MATSA) removed by enzymatic (papain) digestion of Marek\u0026amp;#39;s disease tumour cells was fractionated by gel filtration chromatography. The first peak (F1) was used to raise antibody in rabbits. Monoclonal antibody (RPH-6) directed against MATSA and the anti-F1 IgG were used as idiotypic antibodies to raise polyclonal anti-idiotype serum in heterologous hosts; rabbit and goat, respectively. The anti-idiotypes (anti-Id) were purified by affinity chromatography and characterized by competitive binding assay using immunofluorescent (IF) tests. Day-old white Leghorn chicks were immunized with anti-Id to MATSA (Group 1) or anti-Id to F1 (Group 3) and challenged with virulent Marek\u0026amp;#39;s disease virus (MDV) on the tenth day post immunization. In positive control groups, the day-old chicks were inoculated with anti-BALB/c mouse globulin (Group 2) and anti-rabbit globulin (Group 4) and challenged with virulent MDV on the tenth day post inoculation. As compared with positive control groups, the vaccinated groups (1 and 3) had considerably lower level of MATSA positive cells during the post challenge observation period. The protection level against MD in the immunized groups was 66.6% (Group 1) and 86.6% (Group 3).","publication_date":{"day":null,"month":null,"year":1994,"errors":{}},"publication_name":"Veterinary Immunology and Immunopathology"},"translated_abstract":"Marek\u0026amp;#39;s disease-associated tumour surface antigen (MATSA) removed by enzymatic (papain) digestion of Marek\u0026amp;#39;s disease tumour cells was fractionated by gel filtration chromatography. The first peak (F1) was used to raise antibody in rabbits. Monoclonal antibody (RPH-6) directed against MATSA and the anti-F1 IgG were used as idiotypic antibodies to raise polyclonal anti-idiotype serum in heterologous hosts; rabbit and goat, respectively. The anti-idiotypes (anti-Id) were purified by affinity chromatography and characterized by competitive binding assay using immunofluorescent (IF) tests. Day-old white Leghorn chicks were immunized with anti-Id to MATSA (Group 1) or anti-Id to F1 (Group 3) and challenged with virulent Marek\u0026amp;#39;s disease virus (MDV) on the tenth day post immunization. In positive control groups, the day-old chicks were inoculated with anti-BALB/c mouse globulin (Group 2) and anti-rabbit globulin (Group 4) and challenged with virulent MDV on the tenth day post inoculation. As compared with positive control groups, the vaccinated groups (1 and 3) had considerably lower level of MATSA positive cells during the post challenge observation period. The protection level against MD in the immunized groups was 66.6% (Group 1) and 86.6% (Group 3).","internal_url":"https://www.academia.edu/17119387/Anti_idiotype_antibodies_to_Mareks_disease_associated_tumour_surface_antigen_in_protection_against_Mareks_disease","translated_internal_url":"","created_at":"2015-10-21T11:12:55.070-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":36693995,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":7717084,"work_id":17119387,"tagging_user_id":36693995,"tagged_user_id":12271862,"co_author_invite_id":null,"email":"g***y@gmail.com","display_order":0,"name":"Gagan Mohanty","title":"Anti-idiotype antibodies to Marek's disease-associated tumour surface antigen in protection against Marek's disease"},{"id":7717091,"work_id":17119387,"tagging_user_id":36693995,"tagged_user_id":null,"co_author_invite_id":1723478,"email":"s***t@hotmail.com","display_order":4194304,"name":"S. Dandapat","title":"Anti-idiotype antibodies to Marek's disease-associated tumour surface antigen in protection against Marek's disease"}],"downloadable_attachments":[],"slug":"Anti_idiotype_antibodies_to_Mareks_disease_associated_tumour_surface_antigen_in_protection_against_Mareks_disease","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":36693995,"first_name":"Hare","middle_initials":"","last_name":"Pradhan","page_name":"HarePradhan","domain_name":"independent","created_at":"2015-10-21T11:07:05.415-07:00","display_name":"Hare Pradhan","url":"https://independent.academia.edu/HarePradhan"},"attachments":[],"research_interests":[{"id":173,"name":"Zoology","url":"https://www.academia.edu/Documents/in/Zoology"},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice"},{"id":132052,"name":"Marek Disease","url":"https://www.academia.edu/Documents/in/Marek_Disease"},{"id":252969,"name":"Goats","url":"https://www.academia.edu/Documents/in/Goats"},{"id":402759,"name":"Chickens","url":"https://www.academia.edu/Documents/in/Chickens"},{"id":644860,"name":"Veterinary Sciences","url":"https://www.academia.edu/Documents/in/Veterinary_Sciences"},{"id":782120,"name":"Veterinary Immunology","url":"https://www.academia.edu/Documents/in/Veterinary_Immunology"},{"id":788677,"name":"Rabbits","url":"https://www.academia.edu/Documents/in/Rabbits"},{"id":1716403,"name":"immunoglobulin G","url":"https://www.academia.edu/Documents/in/immunoglobulin_G"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="17119386"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/17119386/Immunopathology_of_Mareks_disease_in_quails_presence_of_antinuclear_antibody_and_immune_complex"><img alt="Research paper thumbnail of Immunopathology of Marek's disease in quails: presence of antinuclear antibody and immune complex" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/17119386/Immunopathology_of_Mareks_disease_in_quails_presence_of_antinuclear_antibody_and_immune_complex">Immunopathology of Marek's disease in quails: presence of antinuclear antibody and immune complex</a></div><div class="wp-workCard_item"><span>Veterinary Immunology and Immunopathology</span><span>, 1991</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Antinuclear antibody (ANA), a marker for autoimmune reactions, was detected in the sera of quails...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Antinuclear antibody (ANA), a marker for autoimmune reactions, was detected in the sera of quails with Marek&amp;#39;s disease (MD). The autoantibody was detected 3 weeks after infection in quails infected with chicken Marek&amp;#39;s disease virus and 4 weeks after infection in quails infected with quail Marek&amp;#39;s disease virus. The ANA titers were low and ranged from 10 to 40. A speckled type of nuclear fluorescence was the characteristic staining feature. In addition to the presence of ANA, immune complexes (IC) were also detected in the kidney glomeruli of quail infected with Marek&amp;#39;s disease virus. Initially about 25-30% of the glomeruli in the kidneys of infected quails had IC deposits. In subsequent periods, the amount of IC deposit and the number of glomeruli showing IC also increased considerably. The findings of the present study suggested autoimmunity may play a pathogenic role in MD.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="17119386"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="17119386"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 17119386; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=17119386]").text(description); $(".js-view-count[data-work-id=17119386]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 17119386; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='17119386']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 17119386, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=17119386]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":17119386,"title":"Immunopathology of Marek's disease in quails: presence of antinuclear antibody and immune complex","translated_title":"","metadata":{"abstract":"Antinuclear antibody (ANA), a marker for autoimmune reactions, was detected in the sera of quails with Marek\u0026amp;#39;s disease (MD). The autoantibody was detected 3 weeks after infection in quails infected with chicken Marek\u0026amp;#39;s disease virus and 4 weeks after infection in quails infected with quail Marek\u0026amp;#39;s disease virus. The ANA titers were low and ranged from 10 to 40. A speckled type of nuclear fluorescence was the characteristic staining feature. In addition to the presence of ANA, immune complexes (IC) were also detected in the kidney glomeruli of quail infected with Marek\u0026amp;#39;s disease virus. Initially about 25-30% of the glomeruli in the kidneys of infected quails had IC deposits. In subsequent periods, the amount of IC deposit and the number of glomeruli showing IC also increased considerably. The findings of the present study suggested autoimmunity may play a pathogenic role in MD.","publication_date":{"day":null,"month":null,"year":1991,"errors":{}},"publication_name":"Veterinary Immunology and Immunopathology"},"translated_abstract":"Antinuclear antibody (ANA), a marker for autoimmune reactions, was detected in the sera of quails with Marek\u0026amp;#39;s disease (MD). The autoantibody was detected 3 weeks after infection in quails infected with chicken Marek\u0026amp;#39;s disease virus and 4 weeks after infection in quails infected with quail Marek\u0026amp;#39;s disease virus. The ANA titers were low and ranged from 10 to 40. A speckled type of nuclear fluorescence was the characteristic staining feature. In addition to the presence of ANA, immune complexes (IC) were also detected in the kidney glomeruli of quail infected with Marek\u0026amp;#39;s disease virus. Initially about 25-30% of the glomeruli in the kidneys of infected quails had IC deposits. In subsequent periods, the amount of IC deposit and the number of glomeruli showing IC also increased considerably. The findings of the present study suggested autoimmunity may play a pathogenic role in MD.","internal_url":"https://www.academia.edu/17119386/Immunopathology_of_Mareks_disease_in_quails_presence_of_antinuclear_antibody_and_immune_complex","translated_internal_url":"","created_at":"2015-10-21T11:12:54.950-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":36693995,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":7717061,"work_id":17119386,"tagging_user_id":36693995,"tagged_user_id":235218309,"co_author_invite_id":1723467,"email":"l***l@howard.edu","display_order":0,"name":"Lalita Kaul","title":"Immunopathology of Marek's disease in quails: presence of antinuclear antibody and immune complex"}],"downloadable_attachments":[],"slug":"Immunopathology_of_Mareks_disease_in_quails_presence_of_antinuclear_antibody_and_immune_complex","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":36693995,"first_name":"Hare","middle_initials":"","last_name":"Pradhan","page_name":"HarePradhan","domain_name":"independent","created_at":"2015-10-21T11:07:05.415-07:00","display_name":"Hare Pradhan","url":"https://independent.academia.edu/HarePradhan"},"attachments":[],"research_interests":[{"id":173,"name":"Zoology","url":"https://www.academia.edu/Documents/in/Zoology"},{"id":132052,"name":"Marek Disease","url":"https://www.academia.edu/Documents/in/Marek_Disease"},{"id":323597,"name":"Fluorescent Antibody Technique","url":"https://www.academia.edu/Documents/in/Fluorescent_Antibody_Technique"},{"id":644860,"name":"Veterinary Sciences","url":"https://www.academia.edu/Documents/in/Veterinary_Sciences"},{"id":924535,"name":"Quail","url":"https://www.academia.edu/Documents/in/Quail"},{"id":1482586,"name":"Autoantibodies","url":"https://www.academia.edu/Documents/in/Autoantibodies"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="17119385"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/17119385/Antibody_directed_against_Mareks_disease_associated_tumor_surface_antigen_can_be_eluted_from_Mareks_disease_tumor_cells"><img alt="Research paper thumbnail of Antibody directed against Marek's disease-associated tumor surface antigen can be eluted from Marek's disease tumor cells" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/17119385/Antibody_directed_against_Mareks_disease_associated_tumor_surface_antigen_can_be_eluted_from_Mareks_disease_tumor_cells">Antibody directed against Marek's disease-associated tumor surface antigen can be eluted from Marek's disease tumor cells</a></div><div class="wp-workCard_item"><span>Veterinary Immunology and Immunopathology</span><span>, 1991</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Antibody directed against Marek&amp;#39;s disease-associated tumor surface antigen (MATSA) was el...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Antibody directed against Marek&amp;#39;s disease-associated tumor surface antigen (MATSA) was eluted from tumor cells of lymphomas and peripheral blood lymphocytes that were isolated from Marek&amp;#39;s disease virus-infected chickens. Feather follicular Marek&amp;#39;s disease virus (MDV) antigen could not be demonstrated with this antibody by indirect immunofluorescent (IF) staining. Monoclonal antibody directed against MATSA could completely block the activity of eluted antibody and vice versa. By indirect IF staining using eluted antibody and fluorescein isothiocyanate (FITC) labelled antichicken globulin conjugate. MATSA-bearing cells were detected in MDV infected and herpes virus of turkey (HVT) vaccinated birds. Blocking of immunoglobulin molecules present on B-cells by anti-chicken globulin is critical in this test.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="17119385"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="17119385"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 17119385; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=17119385]").text(description); $(".js-view-count[data-work-id=17119385]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 17119385; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='17119385']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 17119385, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=17119385]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":17119385,"title":"Antibody directed against Marek's disease-associated tumor surface antigen can be eluted from Marek's disease tumor cells","translated_title":"","metadata":{"abstract":"Antibody directed against Marek\u0026amp;#39;s disease-associated tumor surface antigen (MATSA) was eluted from tumor cells of lymphomas and peripheral blood lymphocytes that were isolated from Marek\u0026amp;#39;s disease virus-infected chickens. Feather follicular Marek\u0026amp;#39;s disease virus (MDV) antigen could not be demonstrated with this antibody by indirect immunofluorescent (IF) staining. Monoclonal antibody directed against MATSA could completely block the activity of eluted antibody and vice versa. By indirect IF staining using eluted antibody and fluorescein isothiocyanate (FITC) labelled antichicken globulin conjugate. MATSA-bearing cells were detected in MDV infected and herpes virus of turkey (HVT) vaccinated birds. Blocking of immunoglobulin molecules present on B-cells by anti-chicken globulin is critical in this test.","publication_date":{"day":null,"month":null,"year":1991,"errors":{}},"publication_name":"Veterinary Immunology and Immunopathology"},"translated_abstract":"Antibody directed against Marek\u0026amp;#39;s disease-associated tumor surface antigen (MATSA) was eluted from tumor cells of lymphomas and peripheral blood lymphocytes that were isolated from Marek\u0026amp;#39;s disease virus-infected chickens. Feather follicular Marek\u0026amp;#39;s disease virus (MDV) antigen could not be demonstrated with this antibody by indirect immunofluorescent (IF) staining. Monoclonal antibody directed against MATSA could completely block the activity of eluted antibody and vice versa. By indirect IF staining using eluted antibody and fluorescein isothiocyanate (FITC) labelled antichicken globulin conjugate. MATSA-bearing cells were detected in MDV infected and herpes virus of turkey (HVT) vaccinated birds. Blocking of immunoglobulin molecules present on B-cells by anti-chicken globulin is critical in this test.","internal_url":"https://www.academia.edu/17119385/Antibody_directed_against_Mareks_disease_associated_tumor_surface_antigen_can_be_eluted_from_Mareks_disease_tumor_cells","translated_internal_url":"","created_at":"2015-10-21T11:12:54.831-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":36693995,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":7717028,"work_id":17119385,"tagging_user_id":36693995,"tagged_user_id":16665419,"co_author_invite_id":null,"email":"b***t@gmail.com","display_order":0,"name":"B.Sushant Patnaik","title":"Antibody directed against Marek's disease-associated tumor surface antigen can be eluted from Marek's disease tumor cells"},{"id":7717086,"work_id":17119385,"tagging_user_id":36693995,"tagged_user_id":12271862,"co_author_invite_id":null,"email":"g***y@gmail.com","display_order":4194304,"name":"Gagan Mohanty","title":"Antibody directed against Marek's disease-associated tumor surface antigen can be eluted from Marek's disease tumor cells"},{"id":7717089,"work_id":17119385,"tagging_user_id":36693995,"tagged_user_id":58805523,"co_author_invite_id":1098759,"email":"w***e@ufl.edu","display_order":6291456,"name":"Won Suk Lee","title":"Antibody directed against Marek's disease-associated tumor surface antigen can be eluted from Marek's disease tumor cells"}],"downloadable_attachments":[],"slug":"Antibody_directed_against_Mareks_disease_associated_tumor_surface_antigen_can_be_eluted_from_Mareks_disease_tumor_cells","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":36693995,"first_name":"Hare","middle_initials":"","last_name":"Pradhan","page_name":"HarePradhan","domain_name":"independent","created_at":"2015-10-21T11:07:05.415-07:00","display_name":"Hare Pradhan","url":"https://independent.academia.edu/HarePradhan"},"attachments":[],"research_interests":[{"id":173,"name":"Zoology","url":"https://www.academia.edu/Documents/in/Zoology"},{"id":55269,"name":"Lymphoma","url":"https://www.academia.edu/Documents/in/Lymphoma"},{"id":132052,"name":"Marek Disease","url":"https://www.academia.edu/Documents/in/Marek_Disease"},{"id":147196,"name":"Monoclonal Antibodies","url":"https://www.academia.edu/Documents/in/Monoclonal_Antibodies"},{"id":323597,"name":"Fluorescent Antibody Technique","url":"https://www.academia.edu/Documents/in/Fluorescent_Antibody_Technique"},{"id":402759,"name":"Chickens","url":"https://www.academia.edu/Documents/in/Chickens"},{"id":644860,"name":"Veterinary Sciences","url":"https://www.academia.edu/Documents/in/Veterinary_Sciences"},{"id":782120,"name":"Veterinary Immunology","url":"https://www.academia.edu/Documents/in/Veterinary_Immunology"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="17119384"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/17119384/Immune_Complex_Mediated_Glomerulopathy_in_Mareks_Disease"><img alt="Research paper thumbnail of Immune Complex-Mediated Glomerulopathy in Marek's Disease" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/17119384/Immune_Complex_Mediated_Glomerulopathy_in_Mareks_Disease">Immune Complex-Mediated Glomerulopathy in Marek's Disease</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/HarePradhan">Hare Pradhan</a> and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/KatariaJ">J. Kataria</a></span></div><div class="wp-workCard_item"><span>Veterinary Immunology and Immunopathology</span><span>, 1988</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Chickens infected with Marek&amp;#39;s disease (MD) virus developed immune complex (IC)-mediated ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Chickens infected with Marek&amp;#39;s disease (MD) virus developed immune complex (IC)-mediated glomerulopathy. Fluorescent antibody staining technique using antichicken globulin and antichicken complement was used to demonstrate IC in the kidney glomeruli. During the initial stages of MDV infection, IC deposits were seen on the glomerular basement membrane, but subsequently the entire glomerulus was involved. Mesangial cells also had IC deposits. Chicken complement was demonstrated in the glomeruli which had IC deposits. The number of glomeruli with IC deposition was higher in tumor-bearing birds than in non-tumor-bearing birds. Histologically, kidney lesion were characterized by thickening of basement membrane and proliferation of mesangial cells. It is suggested that IC-mediated glomerulopathy might be one of the major causes of death in MD.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="17119384"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="17119384"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 17119384; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=17119384]").text(description); $(".js-view-count[data-work-id=17119384]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 17119384; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='17119384']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 17119384, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=17119384]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":17119384,"title":"Immune Complex-Mediated Glomerulopathy in Marek's Disease","translated_title":"","metadata":{"abstract":"Chickens infected with Marek\u0026amp;#39;s disease (MD) virus developed immune complex (IC)-mediated glomerulopathy. Fluorescent antibody staining technique using antichicken globulin and antichicken complement was used to demonstrate IC in the kidney glomeruli. During the initial stages of MDV infection, IC deposits were seen on the glomerular basement membrane, but subsequently the entire glomerulus was involved. Mesangial cells also had IC deposits. Chicken complement was demonstrated in the glomeruli which had IC deposits. The number of glomeruli with IC deposition was higher in tumor-bearing birds than in non-tumor-bearing birds. Histologically, kidney lesion were characterized by thickening of basement membrane and proliferation of mesangial cells. It is suggested that IC-mediated glomerulopathy might be one of the major causes of death in MD.","publication_date":{"day":null,"month":null,"year":1988,"errors":{}},"publication_name":"Veterinary Immunology and Immunopathology"},"translated_abstract":"Chickens infected with Marek\u0026amp;#39;s disease (MD) virus developed immune complex (IC)-mediated glomerulopathy. Fluorescent antibody staining technique using antichicken globulin and antichicken complement was used to demonstrate IC in the kidney glomeruli. During the initial stages of MDV infection, IC deposits were seen on the glomerular basement membrane, but subsequently the entire glomerulus was involved. Mesangial cells also had IC deposits. Chicken complement was demonstrated in the glomeruli which had IC deposits. The number of glomeruli with IC deposition was higher in tumor-bearing birds than in non-tumor-bearing birds. Histologically, kidney lesion were characterized by thickening of basement membrane and proliferation of mesangial cells. It is suggested that IC-mediated glomerulopathy might be one of the major causes of death in MD.","internal_url":"https://www.academia.edu/17119384/Immune_Complex_Mediated_Glomerulopathy_in_Mareks_Disease","translated_internal_url":"","created_at":"2015-10-21T11:12:54.729-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":36693995,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":7717060,"work_id":17119384,"tagging_user_id":36693995,"tagged_user_id":235218309,"co_author_invite_id":1723467,"email":"l***l@howard.edu","display_order":0,"name":"Lalita Kaul","title":"Immune Complex-Mediated Glomerulopathy in Marek's Disease"},{"id":7717085,"work_id":17119384,"tagging_user_id":36693995,"tagged_user_id":12271862,"co_author_invite_id":null,"email":"g***y@gmail.com","display_order":4194304,"name":"Gagan Mohanty","title":"Immune Complex-Mediated Glomerulopathy in Marek's Disease"},{"id":7717088,"work_id":17119384,"tagging_user_id":36693995,"tagged_user_id":58805523,"co_author_invite_id":1098759,"email":"w***e@ufl.edu","display_order":6291456,"name":"Won Suk Lee","title":"Immune Complex-Mediated Glomerulopathy in Marek's Disease"},{"id":7717090,"work_id":17119384,"tagging_user_id":36693995,"tagged_user_id":36804842,"co_author_invite_id":1723477,"email":"j***a@rediffmail.com","display_order":7340032,"name":"J. Kataria","title":"Immune Complex-Mediated Glomerulopathy in Marek's Disease"}],"downloadable_attachments":[],"slug":"Immune_Complex_Mediated_Glomerulopathy_in_Mareks_Disease","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":36693995,"first_name":"Hare","middle_initials":"","last_name":"Pradhan","page_name":"HarePradhan","domain_name":"independent","created_at":"2015-10-21T11:07:05.415-07:00","display_name":"Hare Pradhan","url":"https://independent.academia.edu/HarePradhan"},"attachments":[],"research_interests":[{"id":173,"name":"Zoology","url":"https://www.academia.edu/Documents/in/Zoology"},{"id":132052,"name":"Marek Disease","url":"https://www.academia.edu/Documents/in/Marek_Disease"},{"id":323597,"name":"Fluorescent Antibody Technique","url":"https://www.academia.edu/Documents/in/Fluorescent_Antibody_Technique"},{"id":366620,"name":"POULTRY DISEASES","url":"https://www.academia.edu/Documents/in/POULTRY_DISEASES"},{"id":402759,"name":"Chickens","url":"https://www.academia.edu/Documents/in/Chickens"},{"id":644860,"name":"Veterinary Sciences","url":"https://www.academia.edu/Documents/in/Veterinary_Sciences"},{"id":782120,"name":"Veterinary Immunology","url":"https://www.academia.edu/Documents/in/Veterinary_Immunology"},{"id":1601249,"name":"Glomerulonephritis","url":"https://www.academia.edu/Documents/in/Glomerulonephritis"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="17119383"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/17119383/The_safety_and_efficacy_of_the_oral_rabies_vaccine_SAG2_in_Indian_stray_dogs"><img alt="Research paper thumbnail of The safety and efficacy of the oral rabies vaccine SAG2 in Indian stray dogs" class="work-thumbnail" src="https://attachments.academia-assets.com/42316768/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/17119383/The_safety_and_efficacy_of_the_oral_rabies_vaccine_SAG2_in_Indian_stray_dogs">The safety and efficacy of the oral rabies vaccine SAG2 in Indian stray dogs</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/HarePradhan">Hare Pradhan</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/FlorenceCliquet">Florence Cliquet</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/JGurbuxani">J. Gurbuxani</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/RaghvendarSingh">Raghvendar Singh</a>, and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/CharlesRupprecht">Charles Rupprecht</a></span></div><div class="wp-workCard_item"><span>Vaccine</span><span>, 2007</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="cacb55fc4bf397f0617d455f9bc5dfe2" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":42316768,"asset_id":17119383,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/42316768/download_file?st=MTczMzE5NzgyMSw4LjIyMi4yMDguMTQ2&st=MTczMzE5NzgyMSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="17119383"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="17119383"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 17119383; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=17119383]").text(description); $(".js-view-count[data-work-id=17119383]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 17119383; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='17119383']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 17119383, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "cacb55fc4bf397f0617d455f9bc5dfe2" } } $('.js-work-strip[data-work-id=17119383]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":17119383,"title":"The safety and efficacy of the oral rabies vaccine SAG2 in Indian stray dogs","translated_title":"","metadata":{"ai_title_tag":"Safety and Efficacy of Oral Rabies Vaccine SAG2 in Stray Dogs","grobid_abstract":"India is one of the countries with the highest prevalence of human rabies throughout the world. Dogs are primarily responsible for rabies transmission. Among them, stray dogs play a major role in that country. Parenteral vaccination programmes are insufficient to eliminate rabies partly due to difficulties in establishing satisfactory immunisation coverage in the dog population in view of the high proportion of stray dogs. Oral vaccination may be a useful adjunct to parenteral vaccination by increasing dog vaccination coverage. Safety, immunogenicity and efficacy of Rabidog ® SAG2 bait were evaluated in Indian stray dogs in captivity.","publication_date":{"day":null,"month":null,"year":2007,"errors":{}},"publication_name":"Vaccine","grobid_abstract_attachment_id":42316768},"translated_abstract":null,"internal_url":"https://www.academia.edu/17119383/The_safety_and_efficacy_of_the_oral_rabies_vaccine_SAG2_in_Indian_stray_dogs","translated_internal_url":"","created_at":"2015-10-21T11:12:54.627-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":36693995,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":7717019,"work_id":17119383,"tagging_user_id":36693995,"tagged_user_id":null,"co_author_invite_id":124581,"email":"p***b@gmail.com","display_order":0,"name":"B. Pattnaik","title":"The safety and efficacy of the oral rabies vaccine SAG2 in Indian stray dogs"},{"id":7717025,"work_id":17119383,"tagging_user_id":36693995,"tagged_user_id":7223595,"co_author_invite_id":null,"email":"d***l@gmail.com","display_order":4194304,"name":"Suresh Patil","title":"The safety and efficacy of the oral rabies vaccine SAG2 in Indian stray dogs"},{"id":7717052,"work_id":17119383,"tagging_user_id":36693995,"tagged_user_id":29844908,"co_author_invite_id":null,"email":"r***d@icar.gov.in","display_order":6291456,"name":"Richa Sood","title":"The safety and efficacy of the oral rabies vaccine SAG2 in Indian stray dogs"},{"id":7717071,"work_id":17119383,"tagging_user_id":36693995,"tagged_user_id":36906653,"co_author_invite_id":45757,"email":"f***t@anses.fr","display_order":7340032,"name":"Florence Cliquet","title":"The safety and efficacy of the oral rabies vaccine SAG2 in Indian stray dogs"},{"id":7717072,"work_id":17119383,"tagging_user_id":36693995,"tagged_user_id":36845280,"co_author_invite_id":1723472,"email":"j***x@gmail.com","display_order":7864320,"name":"J. Gurbuxani","title":"The safety and efficacy of the oral rabies vaccine SAG2 in Indian stray dogs"},{"id":7717073,"work_id":17119383,"tagging_user_id":36693995,"tagged_user_id":35746427,"co_author_invite_id":null,"email":"a***t@inserm.fr","display_order":8126464,"name":"Armelle Regnault","title":"The safety and efficacy of the oral rabies vaccine SAG2 in Indian stray dogs"},{"id":7717074,"work_id":17119383,"tagging_user_id":36693995,"tagged_user_id":null,"co_author_invite_id":1723473,"email":"a***t@bpg.it","display_order":8257536,"name":"A. Guiot","title":"The safety and efficacy of the oral rabies vaccine SAG2 in Indian stray dogs"},{"id":7717075,"work_id":17119383,"tagging_user_id":36693995,"tagged_user_id":null,"co_author_invite_id":1723474,"email":"p***l@virbac.fr","display_order":8323072,"name":"P. Mahl","title":"The safety and efficacy of the oral rabies vaccine SAG2 in Indian stray dogs"},{"id":7717076,"work_id":17119383,"tagging_user_id":36693995,"tagged_user_id":33107915,"co_author_invite_id":null,"email":"r***r@gmail.com","display_order":8355840,"name":"Raghvendar Singh","title":"The safety and efficacy of the oral rabies vaccine SAG2 in Indian stray dogs"},{"id":7717077,"work_id":17119383,"tagging_user_id":36693995,"tagged_user_id":null,"co_author_invite_id":1259030,"email":"m***f@who.int","display_order":8372224,"name":"F. Meslin","title":"The safety and efficacy of the oral rabies vaccine SAG2 in Indian stray dogs"},{"id":7717078,"work_id":17119383,"tagging_user_id":36693995,"tagged_user_id":null,"co_author_invite_id":1723475,"email":"e***p@gmail.com","display_order":8380416,"name":"E. Picard","title":"The safety and efficacy of the oral rabies vaccine SAG2 in Indian stray dogs"},{"id":7717079,"work_id":17119383,"tagging_user_id":36693995,"tagged_user_id":null,"co_author_invite_id":1723476,"email":"m***t@iiwelding.org","display_order":8384512,"name":"M. Aubert","title":"The safety and efficacy of the oral rabies vaccine SAG2 in Indian stray dogs"},{"id":7717080,"work_id":17119383,"tagging_user_id":36693995,"tagged_user_id":null,"co_author_invite_id":1624284,"email":"j***t@ifa-fiv.org","display_order":8386560,"name":"J. Barrat","title":"The safety and efficacy of the oral rabies vaccine SAG2 in Indian stray dogs"},{"id":7717081,"work_id":17119383,"tagging_user_id":36693995,"tagged_user_id":24656179,"co_author_invite_id":null,"email":"m***r@gmail.com","display_order":8387584,"name":"Muu Nir","title":"The safety and efficacy of the oral rabies vaccine SAG2 in Indian stray dogs"},{"id":7717082,"work_id":17119383,"tagging_user_id":36693995,"tagged_user_id":36153531,"co_author_invite_id":null,"email":"j***y@envt.fr","display_order":8388096,"name":"Jean-denis Bailly","title":"The safety and efficacy of the oral rabies vaccine SAG2 in Indian stray dogs"},{"id":7717083,"work_id":17119383,"tagging_user_id":36693995,"tagged_user_id":36820555,"co_author_invite_id":387346,"email":"c***t@yahoo.com","display_order":8388352,"name":"Charles Rupprecht","title":"The safety and efficacy of the oral rabies vaccine SAG2 in Indian stray dogs"}],"downloadable_attachments":[{"id":42316768,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/42316768/thumbnails/1.jpg","file_name":"The_safety_and_efficacy_of_the_oral_rabi20160207-27897-lnztl7.pdf","download_url":"https://www.academia.edu/attachments/42316768/download_file?st=MTczMzE5NzgyMSw4LjIyMi4yMDguMTQ2&st=MTczMzE5NzgyMSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"The_safety_and_efficacy_of_the_oral_rabi.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/42316768/The_safety_and_efficacy_of_the_oral_rabi20160207-27897-lnztl7-libre.pdf?1454868700=\u0026response-content-disposition=attachment%3B+filename%3DThe_safety_and_efficacy_of_the_oral_rabi.pdf\u0026Expires=1733201421\u0026Signature=dA7iIN9Nw5uJMFOfgmKLsmx4r8LbHG7UEkY0St~4ht3nYuOISp6qWKoJET19c53oSmploPrBpF1y1La4iM9VCN6aYgPosNOkMQkuJtuzSIfiOSwvwJEtEnJJY9jsvHlWnFJrHUMRfL5qbNfTZjrbwup6Jgd1BKllQG6Fi60mtqF820FteKB8OzwcTWmU6WI834a6kLo5sA~hEi~4WfLs4pyKIBgoA~pP4Hgl3FWN2ARewizpKsHB0qgkJBrR5RwkNMQjubScUa6hHIHfduh4qTO3O0oFrwHH~1FFlZSP21TAopugBYi4t~0rbXYvlV3EWDkoZAMJ6owBeEIWfHJbPA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"The_safety_and_efficacy_of_the_oral_rabies_vaccine_SAG2_in_Indian_stray_dogs","translated_slug":"","page_count":10,"language":"en","content_type":"Work","owner":{"id":36693995,"first_name":"Hare","middle_initials":"","last_name":"Pradhan","page_name":"HarePradhan","domain_name":"independent","created_at":"2015-10-21T11:07:05.415-07:00","display_name":"Hare Pradhan","url":"https://independent.academia.edu/HarePradhan"},"attachments":[{"id":42316768,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/42316768/thumbnails/1.jpg","file_name":"The_safety_and_efficacy_of_the_oral_rabi20160207-27897-lnztl7.pdf","download_url":"https://www.academia.edu/attachments/42316768/download_file?st=MTczMzE5NzgyMSw4LjIyMi4yMDguMTQ2&st=MTczMzE5NzgyMSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"The_safety_and_efficacy_of_the_oral_rabi.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/42316768/The_safety_and_efficacy_of_the_oral_rabi20160207-27897-lnztl7-libre.pdf?1454868700=\u0026response-content-disposition=attachment%3B+filename%3DThe_safety_and_efficacy_of_the_oral_rabi.pdf\u0026Expires=1733201421\u0026Signature=dA7iIN9Nw5uJMFOfgmKLsmx4r8LbHG7UEkY0St~4ht3nYuOISp6qWKoJET19c53oSmploPrBpF1y1La4iM9VCN6aYgPosNOkMQkuJtuzSIfiOSwvwJEtEnJJY9jsvHlWnFJrHUMRfL5qbNfTZjrbwup6Jgd1BKllQG6Fi60mtqF820FteKB8OzwcTWmU6WI834a6kLo5sA~hEi~4WfLs4pyKIBgoA~pP4Hgl3FWN2ARewizpKsHB0qgkJBrR5RwkNMQjubScUa6hHIHfduh4qTO3O0oFrwHH~1FFlZSP21TAopugBYi4t~0rbXYvlV3EWDkoZAMJ6owBeEIWfHJbPA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":1199,"name":"Eastern Europe","url":"https://www.academia.edu/Documents/in/Eastern_Europe"},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences"},{"id":49161,"name":"Safety","url":"https://www.academia.edu/Documents/in/Safety"},{"id":52438,"name":"Dogs","url":"https://www.academia.edu/Documents/in/Dogs"},{"id":150847,"name":"Vaccine","url":"https://www.academia.edu/Documents/in/Vaccine"},{"id":179934,"name":"Vaccination","url":"https://www.academia.edu/Documents/in/Vaccination"},{"id":289330,"name":"Prevalence","url":"https://www.academia.edu/Documents/in/Prevalence"},{"id":344106,"name":"Saliva","url":"https://www.academia.edu/Documents/in/Saliva"},{"id":483642,"name":"Rabies Vaccines","url":"https://www.academia.edu/Documents/in/Rabies_Vaccines"},{"id":966167,"name":"Salivary Gland","url":"https://www.academia.edu/Documents/in/Salivary_Gland"},{"id":988725,"name":"Clinical Signs","url":"https://www.academia.edu/Documents/in/Clinical_Signs"},{"id":1010136,"name":"Rabies Virus","url":"https://www.academia.edu/Documents/in/Rabies_Virus"},{"id":1703190,"name":"Neutralizing antibodies","url":"https://www.academia.edu/Documents/in/Neutralizing_antibodies"},{"id":1944473,"name":"Oral Vaccination","url":"https://www.academia.edu/Documents/in/Oral_Vaccination"}],"urls":[{"id":6522415,"url":"https://www.researchgate.net/profile/Richa_Sood2/publication/6578468_The_safety_and_efficacy_of_the_oral_rabies_vaccine_SAG2_in_Indian_stray_dogs/links/542693f20cf26120b7b0b065.pdf"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="17119382"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/17119382/Outbreaks_of_egg_drop_syndrome_due_to_EDS_76_virus_in_quail_Coturnix_coturnix_japonica_"><img alt="Research paper thumbnail of Outbreaks of egg drop syndrome due to EDS-76 virus in quail (Coturnix coturnix japonica)" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/17119382/Outbreaks_of_egg_drop_syndrome_due_to_EDS_76_virus_in_quail_Coturnix_coturnix_japonica_">Outbreaks of egg drop syndrome due to EDS-76 virus in quail (Coturnix coturnix japonica)</a></div><div class="wp-workCard_item"><span>Veterinary Record</span><span>, 1992</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Two outbreaks of the egg drop syndrome were observed in quail flocks maintained on a farm togethe...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Two outbreaks of the egg drop syndrome were observed in quail flocks maintained on a farm together with chickens. The decrease in egg production ranged from 10.6 per cent to 50.6 per cent, and the number of soft-shelled eggs increased with the decline in egg production. In both the outbreaks haemagglutination inhibiting antibodies to EDS-76 virus were detected. Fluorescent viral antigen specific to EDS-76 virus was also detected in the lining epithelial and glandular cells of the uterus.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="17119382"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="17119382"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 17119382; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=17119382]").text(description); $(".js-view-count[data-work-id=17119382]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 17119382; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='17119382']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 17119382, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=17119382]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":17119382,"title":"Outbreaks of egg drop syndrome due to EDS-76 virus in quail (Coturnix coturnix japonica)","translated_title":"","metadata":{"abstract":"Two outbreaks of the egg drop syndrome were observed in quail flocks maintained on a farm together with chickens. The decrease in egg production ranged from 10.6 per cent to 50.6 per cent, and the number of soft-shelled eggs increased with the decline in egg production. In both the outbreaks haemagglutination inhibiting antibodies to EDS-76 virus were detected. Fluorescent viral antigen specific to EDS-76 virus was also detected in the lining epithelial and glandular cells of the uterus.","publication_date":{"day":null,"month":null,"year":1992,"errors":{}},"publication_name":"Veterinary Record"},"translated_abstract":"Two outbreaks of the egg drop syndrome were observed in quail flocks maintained on a farm together with chickens. The decrease in egg production ranged from 10.6 per cent to 50.6 per cent, and the number of soft-shelled eggs increased with the decline in egg production. In both the outbreaks haemagglutination inhibiting antibodies to EDS-76 virus were detected. Fluorescent viral antigen specific to EDS-76 virus was also detected in the lining epithelial and glandular cells of the uterus.","internal_url":"https://www.academia.edu/17119382/Outbreaks_of_egg_drop_syndrome_due_to_EDS_76_virus_in_quail_Coturnix_coturnix_japonica_","translated_internal_url":"","created_at":"2015-10-21T11:12:54.536-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":36693995,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":7717070,"work_id":17119382,"tagging_user_id":36693995,"tagged_user_id":28628339,"co_author_invite_id":null,"email":"b***y@gmail.com","affiliation":"West Bengal University of Animal and Fishery Sciences","display_order":0,"name":"Bipul Kumar Das","title":"Outbreaks of egg drop syndrome due to EDS-76 virus in quail (Coturnix coturnix japonica)"}],"downloadable_attachments":[],"slug":"Outbreaks_of_egg_drop_syndrome_due_to_EDS_76_virus_in_quail_Coturnix_coturnix_japonica_","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":36693995,"first_name":"Hare","middle_initials":"","last_name":"Pradhan","page_name":"HarePradhan","domain_name":"independent","created_at":"2015-10-21T11:07:05.415-07:00","display_name":"Hare Pradhan","url":"https://independent.academia.edu/HarePradhan"},"attachments":[],"research_interests":[{"id":43838,"name":"India","url":"https://www.academia.edu/Documents/in/India"},{"id":57907,"name":"Disease Outbreaks","url":"https://www.academia.edu/Documents/in/Disease_Outbreaks"},{"id":366620,"name":"POULTRY DISEASES","url":"https://www.academia.edu/Documents/in/POULTRY_DISEASES"},{"id":499699,"name":"Oviposition","url":"https://www.academia.edu/Documents/in/Oviposition"},{"id":644860,"name":"Veterinary Sciences","url":"https://www.academia.edu/Documents/in/Veterinary_Sciences"},{"id":1225171,"name":"Syndrome","url":"https://www.academia.edu/Documents/in/Syndrome"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="17119381"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/17119381/Identification_of_bovine_viral_diarrhea_virus_type_1_in_yaks_Bos_poephagus_grunniens_in_the_Himalayan_region"><img alt="Research paper thumbnail of Identification of bovine viral diarrhea virus type 1 in yaks (Bos poephagus grunniens) in the Himalayan region" class="work-thumbnail" src="https://attachments.academia-assets.com/42316754/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/17119381/Identification_of_bovine_viral_diarrhea_virus_type_1_in_yaks_Bos_poephagus_grunniens_in_the_Himalayan_region">Identification of bovine viral diarrhea virus type 1 in yaks (Bos poephagus grunniens) in the Himalayan region</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/HarePradhan">Hare Pradhan</a> and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/ATiwari1">A. Tiwari</a></span></div><div class="wp-workCard_item"><span>Research in Veterinary Science</span><span>, 2008</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="3899d6437447c9fcd3bf11e3c59ce8db" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":42316754,"asset_id":17119381,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/42316754/download_file?st=MTczMzE5NzgyMSw4LjIyMi4yMDguMTQ2&st=MTczMzE5NzgyMSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="17119381"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="17119381"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 17119381; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=17119381]").text(description); $(".js-view-count[data-work-id=17119381]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 17119381; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='17119381']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 17119381, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "3899d6437447c9fcd3bf11e3c59ce8db" } } $('.js-work-strip[data-work-id=17119381]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":17119381,"title":"Identification of bovine viral diarrhea virus type 1 in yaks (Bos poephagus grunniens) in the Himalayan region","translated_title":"","metadata":{"grobid_abstract":"Since cattle are widely infected by bovine viral diarrhea virus (BVDV) in India, we searched for pestivirus infection in yaks. Of 71 pure and crossbred yaks from Himalayan region, pestivirus antigen was detected by Ag-ELISA in three animals. Pestivirus in leukocyte and cell culture isolated virus samples originating from positive yaks was also confirmed by RT-PCR using panpestivirus specific primers selected from 5 0 -untranslated region (5 0 UTR). The 5 0 UTR, N pro and E2 regions were sequenced and used for genetic typing. Phylogenetic analysis revealed that pestiviruses detected in three Himalayan yaks were similar genetically, belonging to BVDV-1. Antigenic characterisation of yak pestivirus also confirmed the typing as BVDV-1. This is the first report on the identification of BVDV type 1 in yaks.","publication_date":{"day":null,"month":null,"year":2008,"errors":{}},"publication_name":"Research in Veterinary Science","grobid_abstract_attachment_id":42316754},"translated_abstract":null,"internal_url":"https://www.academia.edu/17119381/Identification_of_bovine_viral_diarrhea_virus_type_1_in_yaks_Bos_poephagus_grunniens_in_the_Himalayan_region","translated_internal_url":"","created_at":"2015-10-21T11:12:54.428-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":36693995,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":7717032,"work_id":17119381,"tagging_user_id":36693995,"tagged_user_id":50823026,"co_author_invite_id":616470,"email":"m***r@rediffmail.com","display_order":0,"name":"Niranjan Mishra","title":"Identification of bovine viral diarrhea virus type 1 in yaks (Bos poephagus grunniens) in the Himalayan region"},{"id":7717062,"work_id":17119381,"tagging_user_id":36693995,"tagged_user_id":null,"co_author_invite_id":1723468,"email":"k***r@rediffmail.com","display_order":4194304,"name":"K. Rajukumar","title":"Identification of bovine viral diarrhea virus type 1 in yaks (Bos poephagus grunniens) in the Himalayan region"},{"id":7717065,"work_id":17119381,"tagging_user_id":36693995,"tagged_user_id":null,"co_author_invite_id":1723470,"email":"r***u@rediffmail.com","display_order":6291456,"name":"R. Dubey","title":"Identification of bovine viral diarrhea virus type 1 in yaks (Bos poephagus grunniens) in the Himalayan region"},{"id":7717067,"work_id":17119381,"tagging_user_id":36693995,"tagged_user_id":36958264,"co_author_invite_id":1723471,"email":"a***3@yahoo.com","display_order":7340032,"name":"A. Tiwari","title":"Identification of bovine viral diarrhea virus type 1 in yaks (Bos poephagus grunniens) in the Himalayan region"},{"id":7717069,"work_id":17119381,"tagging_user_id":36693995,"tagged_user_id":10236221,"co_author_invite_id":null,"email":"v***v@rediffmail.com","display_order":7864320,"name":"Alka Galav","title":"Identification of bovine viral diarrhea virus type 1 in yaks (Bos poephagus grunniens) in the Himalayan region"},{"id":7717098,"work_id":17119381,"tagging_user_id":36693995,"tagged_user_id":33035758,"co_author_invite_id":null,"email":"v***k@uvm.sk","display_order":8126464,"name":"Stefan Vilcek","title":"Identification of bovine viral diarrhea virus type 1 in yaks (Bos poephagus grunniens) in the Himalayan region"}],"downloadable_attachments":[{"id":42316754,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/42316754/thumbnails/1.jpg","file_name":"00b7d5281ff459e88f000000.pdf","download_url":"https://www.academia.edu/attachments/42316754/download_file?st=MTczMzE5NzgyMSw4LjIyMi4yMDguMTQ2&st=MTczMzE5NzgyMSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Identification_of_bovine_viral_diarrhea.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/42316754/00b7d5281ff459e88f000000-libre.pdf?1454868701=\u0026response-content-disposition=attachment%3B+filename%3DIdentification_of_bovine_viral_diarrhea.pdf\u0026Expires=1733201421\u0026Signature=EohykNIbxuLCewRZjlZMyJL1h0tkYBV6px~G~B4DmezBXX2hnMjz7u3byHWJ0YNpIWtyPseth8dNc3NNpFG6ra-8M0xEESILGfauWVEYY6faNumcOLKfSHryP2dbgyPxiHJWjPzsA5qEZdkapY0HhYsktDuKNycd67G4nz~18boxB4pAppAebF6-mp~ne3uazRKnKEPF0BM0CdQa-5c8u6vGhu7eVdZ1~KCYfMJzJ3oSEwpI0lZyJNIzXv8yS0grfAgOyiEwS4Y8I7UmfXHHe7DvAlwUXwlewBBHEqLzT1op8KziODS2~8FMQaZL~6lX~oxCF99~DV-Bzhxqvm2BJw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Identification_of_bovine_viral_diarrhea_virus_type_1_in_yaks_Bos_poephagus_grunniens_in_the_Himalayan_region","translated_slug":"","page_count":4,"language":"en","content_type":"Work","owner":{"id":36693995,"first_name":"Hare","middle_initials":"","last_name":"Pradhan","page_name":"HarePradhan","domain_name":"independent","created_at":"2015-10-21T11:07:05.415-07:00","display_name":"Hare Pradhan","url":"https://independent.academia.edu/HarePradhan"},"attachments":[{"id":42316754,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/42316754/thumbnails/1.jpg","file_name":"00b7d5281ff459e88f000000.pdf","download_url":"https://www.academia.edu/attachments/42316754/download_file?st=MTczMzE5NzgyMSw4LjIyMi4yMDguMTQ2&st=MTczMzE5NzgyMSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Identification_of_bovine_viral_diarrhea.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/42316754/00b7d5281ff459e88f000000-libre.pdf?1454868701=\u0026response-content-disposition=attachment%3B+filename%3DIdentification_of_bovine_viral_diarrhea.pdf\u0026Expires=1733201421\u0026Signature=EohykNIbxuLCewRZjlZMyJL1h0tkYBV6px~G~B4DmezBXX2hnMjz7u3byHWJ0YNpIWtyPseth8dNc3NNpFG6ra-8M0xEESILGfauWVEYY6faNumcOLKfSHryP2dbgyPxiHJWjPzsA5qEZdkapY0HhYsktDuKNycd67G4nz~18boxB4pAppAebF6-mp~ne3uazRKnKEPF0BM0CdQa-5c8u6vGhu7eVdZ1~KCYfMJzJ3oSEwpI0lZyJNIzXv8yS0grfAgOyiEwS4Y8I7UmfXHHe7DvAlwUXwlewBBHEqLzT1op8KziODS2~8FMQaZL~6lX~oxCF99~DV-Bzhxqvm2BJw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":156,"name":"Genetics","url":"https://www.academia.edu/Documents/in/Genetics"},{"id":6697,"name":"Australia","url":"https://www.academia.edu/Documents/in/Australia"},{"id":29980,"name":"Animal Production","url":"https://www.academia.edu/Documents/in/Animal_Production"},{"id":35414,"name":"Veterinary","url":"https://www.academia.edu/Documents/in/Veterinary"},{"id":43294,"name":"Veterinary Science","url":"https://www.academia.edu/Documents/in/Veterinary_Science"},{"id":43838,"name":"India","url":"https://www.academia.edu/Documents/in/India"},{"id":50576,"name":"Germany","url":"https://www.academia.edu/Documents/in/Germany"},{"id":54433,"name":"Phylogeny","url":"https://www.academia.edu/Documents/in/Phylogeny"},{"id":158782,"name":"Phylogenetic analysis","url":"https://www.academia.edu/Documents/in/Phylogenetic_analysis"},{"id":260829,"name":"Cattle","url":"https://www.academia.edu/Documents/in/Cattle"},{"id":644860,"name":"Veterinary Sciences","url":"https://www.academia.edu/Documents/in/Veterinary_Sciences"},{"id":1709309,"name":"Bovine Viral Diarrhea Virus","url":"https://www.academia.edu/Documents/in/Bovine_Viral_Diarrhea_Virus"},{"id":2056389,"name":"Pestivirus","url":"https://www.academia.edu/Documents/in/Pestivirus"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="17119380"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/17119380/Development_and_evaluation_of_a_MAb_based_competitive_ELISA_using_helicase_domain_of_NS3_protein_for_sero_diagnosis_of_bovine_viral_diarrhea_in_cattle_and_buffaloes"><img alt="Research paper thumbnail of Development and evaluation of a MAb based competitive-ELISA using helicase domain of NS3 protein for sero-diagnosis of bovine viral diarrhea in cattle and buffaloes" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/17119380/Development_and_evaluation_of_a_MAb_based_competitive_ELISA_using_helicase_domain_of_NS3_protein_for_sero_diagnosis_of_bovine_viral_diarrhea_in_cattle_and_buffaloes">Development and evaluation of a MAb based competitive-ELISA using helicase domain of NS3 protein for sero-diagnosis of bovine viral diarrhea in cattle and buffaloes</a></div><div class="wp-workCard_item"><span>Research in Veterinary Science</span><span>, 2008</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The aim of this study was to develop a competitive inhibition ELISA (CI-ELISA) for detection of a...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The aim of this study was to develop a competitive inhibition ELISA (CI-ELISA) for detection of antibodies to bovine viral diarrhea virus (BVDV) using the helicase domain of NS3 (non-structural) protein and monoclonal antibody (MAb) against it and to estimate its sensitivity and specificity using two commercial ELISA kits as independent references. The 45-kDa helicase domain of NS3 protein of BVDV was expressed in Escherichia coli and 18MAbs were developed against it. MAb-11G8 was selected for use in CI-ELISA on the basis of maximum inhibition (90%) obtained with BVDV type 1 infected calf serum. Based on the distribution of percent inhibition of known negative sera (n=166), a cut-off value was set at 40% inhibition. In testing 914 field serum samples of cattle (810) and buffaloes (104), the CI-ELISA showed a relative specificity of 95.75% and 97.38% and sensitivity of 96% and 94.43% with Ingenesa kit and Institut Pourquier kit, respectively. This study proved that the use of helicase domain of NS3 (45-kDa) is equally good as the whole NS3 protein (80-kDa) used in commercial kits for detection of BVDV antibodies in cattle and buffaloes.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="17119380"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="17119380"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 17119380; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=17119380]").text(description); $(".js-view-count[data-work-id=17119380]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 17119380; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='17119380']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 17119380, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=17119380]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":17119380,"title":"Development and evaluation of a MAb based competitive-ELISA using helicase domain of NS3 protein for sero-diagnosis of bovine viral diarrhea in cattle and buffaloes","translated_title":"","metadata":{"abstract":"The aim of this study was to develop a competitive inhibition ELISA (CI-ELISA) for detection of antibodies to bovine viral diarrhea virus (BVDV) using the helicase domain of NS3 (non-structural) protein and monoclonal antibody (MAb) against it and to estimate its sensitivity and specificity using two commercial ELISA kits as independent references. The 45-kDa helicase domain of NS3 protein of BVDV was expressed in Escherichia coli and 18MAbs were developed against it. MAb-11G8 was selected for use in CI-ELISA on the basis of maximum inhibition (90%) obtained with BVDV type 1 infected calf serum. Based on the distribution of percent inhibition of known negative sera (n=166), a cut-off value was set at 40% inhibition. In testing 914 field serum samples of cattle (810) and buffaloes (104), the CI-ELISA showed a relative specificity of 95.75% and 97.38% and sensitivity of 96% and 94.43% with Ingenesa kit and Institut Pourquier kit, respectively. This study proved that the use of helicase domain of NS3 (45-kDa) is equally good as the whole NS3 protein (80-kDa) used in commercial kits for detection of BVDV antibodies in cattle and buffaloes.","publication_date":{"day":null,"month":null,"year":2008,"errors":{}},"publication_name":"Research in Veterinary Science"},"translated_abstract":"The aim of this study was to develop a competitive inhibition ELISA (CI-ELISA) for detection of antibodies to bovine viral diarrhea virus (BVDV) using the helicase domain of NS3 (non-structural) protein and monoclonal antibody (MAb) against it and to estimate its sensitivity and specificity using two commercial ELISA kits as independent references. The 45-kDa helicase domain of NS3 protein of BVDV was expressed in Escherichia coli and 18MAbs were developed against it. MAb-11G8 was selected for use in CI-ELISA on the basis of maximum inhibition (90%) obtained with BVDV type 1 infected calf serum. Based on the distribution of percent inhibition of known negative sera (n=166), a cut-off value was set at 40% inhibition. In testing 914 field serum samples of cattle (810) and buffaloes (104), the CI-ELISA showed a relative specificity of 95.75% and 97.38% and sensitivity of 96% and 94.43% with Ingenesa kit and Institut Pourquier kit, respectively. This study proved that the use of helicase domain of NS3 (45-kDa) is equally good as the whole NS3 protein (80-kDa) used in commercial kits for detection of BVDV antibodies in cattle and buffaloes.","internal_url":"https://www.academia.edu/17119380/Development_and_evaluation_of_a_MAb_based_competitive_ELISA_using_helicase_domain_of_NS3_protein_for_sero_diagnosis_of_bovine_viral_diarrhea_in_cattle_and_buffaloes","translated_internal_url":"","created_at":"2015-10-21T11:12:54.320-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":36693995,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":7717023,"work_id":17119380,"tagging_user_id":36693995,"tagged_user_id":null,"co_author_invite_id":124581,"email":"p***b@gmail.com","display_order":0,"name":"B. Pattnaik","title":"Development and evaluation of a MAb based competitive-ELISA using helicase domain of NS3 protein for sero-diagnosis of bovine viral diarrhea in cattle and buffaloes"},{"id":7717035,"work_id":17119380,"tagging_user_id":36693995,"tagged_user_id":50823026,"co_author_invite_id":616470,"email":"m***r@rediffmail.com","display_order":4194304,"name":"Niranjan Mishra","title":"Development and evaluation of a MAb based competitive-ELISA using helicase domain of NS3 protein for sero-diagnosis of bovine viral diarrhea in cattle and buffaloes"},{"id":7717050,"work_id":17119380,"tagging_user_id":36693995,"tagged_user_id":null,"co_author_invite_id":1723465,"email":"b***n@yahoo.co.in","display_order":6291456,"name":"S. Bhatia","title":"Development and evaluation of a MAb based competitive-ELISA using helicase domain of NS3 protein for sero-diagnosis of bovine viral diarrhea in cattle and buffaloes"},{"id":7717054,"work_id":17119380,"tagging_user_id":36693995,"tagged_user_id":29844908,"co_author_invite_id":null,"email":"r***d@icar.gov.in","display_order":7340032,"name":"Richa Sood","title":"Development and evaluation of a MAb based competitive-ELISA using helicase domain of NS3 protein for sero-diagnosis of bovine viral diarrhea in cattle and buffaloes"}],"downloadable_attachments":[],"slug":"Development_and_evaluation_of_a_MAb_based_competitive_ELISA_using_helicase_domain_of_NS3_protein_for_sero_diagnosis_of_bovine_viral_diarrhea_in_cattle_and_buffaloes","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":36693995,"first_name":"Hare","middle_initials":"","last_name":"Pradhan","page_name":"HarePradhan","domain_name":"independent","created_at":"2015-10-21T11:07:05.415-07:00","display_name":"Hare Pradhan","url":"https://independent.academia.edu/HarePradhan"},"attachments":[],"research_interests":[{"id":29980,"name":"Animal Production","url":"https://www.academia.edu/Documents/in/Animal_Production"},{"id":35414,"name":"Veterinary","url":"https://www.academia.edu/Documents/in/Veterinary"},{"id":43294,"name":"Veterinary Science","url":"https://www.academia.edu/Documents/in/Veterinary_Science"},{"id":83128,"name":"Escherichia coli","url":"https://www.academia.edu/Documents/in/Escherichia_coli"},{"id":147196,"name":"Monoclonal Antibodies","url":"https://www.academia.edu/Documents/in/Monoclonal_Antibodies"},{"id":260829,"name":"Cattle","url":"https://www.academia.edu/Documents/in/Cattle"},{"id":644860,"name":"Veterinary Sciences","url":"https://www.academia.edu/Documents/in/Veterinary_Sciences"},{"id":766014,"name":"Monoclonal Antibody","url":"https://www.academia.edu/Documents/in/Monoclonal_Antibody"},{"id":901876,"name":"Sensitivity and Specificity","url":"https://www.academia.edu/Documents/in/Sensitivity_and_Specificity"},{"id":1272906,"name":"Enzyme Linked Immunosorbent Assay","url":"https://www.academia.edu/Documents/in/Enzyme_Linked_Immunosorbent_Assay"},{"id":1709309,"name":"Bovine Viral Diarrhea Virus","url":"https://www.academia.edu/Documents/in/Bovine_Viral_Diarrhea_Virus"},{"id":1793091,"name":"Buffaloes","url":"https://www.academia.edu/Documents/in/Buffaloes"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="17119379"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/17119379/Vaccination_trial_of_quail_with_herpes_virus_of_turkey"><img alt="Research paper thumbnail of Vaccination trial of quail with herpes virus of turkey" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/17119379/Vaccination_trial_of_quail_with_herpes_virus_of_turkey">Vaccination trial of quail with herpes virus of turkey</a></div><div class="wp-workCard_item"><span>Preventive Veterinary Medicine</span><span>, 1991</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Attempts were made to evaluate the efficacy of herpes virus of turkey (HVT) in the protection of ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Attempts were made to evaluate the efficacy of herpes virus of turkey (HVT) in the protection of quail against Marek&amp;#x27;s disease (MD). One hundred and four 1-day-old quail, each vaccinated with 1000 plaque-forming units (PFU) of HVT were challenged 10 days post-vaccination ...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="17119379"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="17119379"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 17119379; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=17119379]").text(description); $(".js-view-count[data-work-id=17119379]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 17119379; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='17119379']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 17119379, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=17119379]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":17119379,"title":"Vaccination trial of quail with herpes virus of turkey","translated_title":"","metadata":{"abstract":"Attempts were made to evaluate the efficacy of herpes virus of turkey (HVT) in the protection of quail against Marek\u0026amp;#x27;s disease (MD). One hundred and four 1-day-old quail, each vaccinated with 1000 plaque-forming units (PFU) of HVT were challenged 10 days post-vaccination ...","publication_date":{"day":null,"month":null,"year":1991,"errors":{}},"publication_name":"Preventive Veterinary Medicine"},"translated_abstract":"Attempts were made to evaluate the efficacy of herpes virus of turkey (HVT) in the protection of quail against Marek\u0026amp;#x27;s disease (MD). One hundred and four 1-day-old quail, each vaccinated with 1000 plaque-forming units (PFU) of HVT were challenged 10 days post-vaccination ...","internal_url":"https://www.academia.edu/17119379/Vaccination_trial_of_quail_with_herpes_virus_of_turkey","translated_internal_url":"","created_at":"2015-10-21T11:12:54.212-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":36693995,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":7717059,"work_id":17119379,"tagging_user_id":36693995,"tagged_user_id":235218309,"co_author_invite_id":1723467,"email":"l***l@howard.edu","display_order":0,"name":"Lalita Kaul","title":"Vaccination trial of quail with herpes virus of turkey"}],"downloadable_attachments":[],"slug":"Vaccination_trial_of_quail_with_herpes_virus_of_turkey","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":36693995,"first_name":"Hare","middle_initials":"","last_name":"Pradhan","page_name":"HarePradhan","domain_name":"independent","created_at":"2015-10-21T11:07:05.415-07:00","display_name":"Hare Pradhan","url":"https://independent.academia.edu/HarePradhan"},"attachments":[],"research_interests":[{"id":43819,"name":"Preventive Veterinary Medicine","url":"https://www.academia.edu/Documents/in/Preventive_Veterinary_Medicine"},{"id":644860,"name":"Veterinary Sciences","url":"https://www.academia.edu/Documents/in/Veterinary_Sciences"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="17119378"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/17119378/Single_chain_fragment_variable_antibody_against_the_capsid_protein_of_bovine_immunodeficiency_virus_and_its_use_in_ELISA"><img alt="Research paper thumbnail of Single-chain fragment variable antibody against the capsid protein of bovine immunodeficiency virus and its use in ELISA" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/17119378/Single_chain_fragment_variable_antibody_against_the_capsid_protein_of_bovine_immunodeficiency_virus_and_its_use_in_ELISA">Single-chain fragment variable antibody against the capsid protein of bovine immunodeficiency virus and its use in ELISA</a></div><div class="wp-workCard_item"><span>Journal of Virological Methods</span><span>, 2010</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Recombinant antibody specific for the capsid (CA) protein of bovine immunodeficiency virus (BIV) ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Recombinant antibody specific for the capsid (CA) protein of bovine immunodeficiency virus (BIV) was generated in the form of single-chain fragment variable (ScFv) using the phage display technique for affinity selection. The variable heavy (V(H)) and variable light (V(L)) chain gene fragments were recovered from cells of CA-specific hybridoma (9G10) described previously. The V(H) and V(L) DNA fragments were assembled through a flexible linker DNA to generate ScFv fragment which was cloned in a phagemid expression vector to express ScFv protein. The specific reactivity of the expressed ScFv to the CA antigen was confirmed by Western blot, and the ScFv fragment was used to develop a competitive inhibition ELISA for detection of antibodies to BIV in cattle and buffalo. The recombinant antibody was shown to be more than four times sensitive than its parent monoclonal antibody (MAb, 9G10) by testing of spiked samples of reference positive sera. The improved sensitivity of the recombinant antibody-based ELISA was confirmed by the detection of a larger proportion of animals with BIV antibody by it than by the MAb-based ELISA.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="17119378"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="17119378"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 17119378; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=17119378]").text(description); $(".js-view-count[data-work-id=17119378]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 17119378; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='17119378']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 17119378, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=17119378]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":17119378,"title":"Single-chain fragment variable antibody against the capsid protein of bovine immunodeficiency virus and its use in ELISA","translated_title":"","metadata":{"abstract":"Recombinant antibody specific for the capsid (CA) protein of bovine immunodeficiency virus (BIV) was generated in the form of single-chain fragment variable (ScFv) using the phage display technique for affinity selection. The variable heavy (V(H)) and variable light (V(L)) chain gene fragments were recovered from cells of CA-specific hybridoma (9G10) described previously. The V(H) and V(L) DNA fragments were assembled through a flexible linker DNA to generate ScFv fragment which was cloned in a phagemid expression vector to express ScFv protein. The specific reactivity of the expressed ScFv to the CA antigen was confirmed by Western blot, and the ScFv fragment was used to develop a competitive inhibition ELISA for detection of antibodies to BIV in cattle and buffalo. The recombinant antibody was shown to be more than four times sensitive than its parent monoclonal antibody (MAb, 9G10) by testing of spiked samples of reference positive sera. The improved sensitivity of the recombinant antibody-based ELISA was confirmed by the detection of a larger proportion of animals with BIV antibody by it than by the MAb-based ELISA.","publication_date":{"day":null,"month":null,"year":2010,"errors":{}},"publication_name":"Journal of Virological Methods"},"translated_abstract":"Recombinant antibody specific for the capsid (CA) protein of bovine immunodeficiency virus (BIV) was generated in the form of single-chain fragment variable (ScFv) using the phage display technique for affinity selection. The variable heavy (V(H)) and variable light (V(L)) chain gene fragments were recovered from cells of CA-specific hybridoma (9G10) described previously. The V(H) and V(L) DNA fragments were assembled through a flexible linker DNA to generate ScFv fragment which was cloned in a phagemid expression vector to express ScFv protein. The specific reactivity of the expressed ScFv to the CA antigen was confirmed by Western blot, and the ScFv fragment was used to develop a competitive inhibition ELISA for detection of antibodies to BIV in cattle and buffalo. The recombinant antibody was shown to be more than four times sensitive than its parent monoclonal antibody (MAb, 9G10) by testing of spiked samples of reference positive sera. The improved sensitivity of the recombinant antibody-based ELISA was confirmed by the detection of a larger proportion of animals with BIV antibody by it than by the MAb-based ELISA.","internal_url":"https://www.academia.edu/17119378/Single_chain_fragment_variable_antibody_against_the_capsid_protein_of_bovine_immunodeficiency_virus_and_its_use_in_ELISA","translated_internal_url":"","created_at":"2015-10-21T11:12:54.080-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":36693995,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":7717049,"work_id":17119378,"tagging_user_id":36693995,"tagged_user_id":null,"co_author_invite_id":1723465,"email":"b***n@yahoo.co.in","display_order":0,"name":"S. Bhatia","title":"Single-chain fragment variable antibody against the capsid protein of bovine immunodeficiency virus and its use in ELISA"},{"id":7717053,"work_id":17119378,"tagging_user_id":36693995,"tagged_user_id":29844908,"co_author_invite_id":null,"email":"r***d@icar.gov.in","display_order":4194304,"name":"Richa Sood","title":"Single-chain fragment variable antibody against the capsid protein of bovine immunodeficiency virus and its use in ELISA"},{"id":7717056,"work_id":17119378,"tagging_user_id":36693995,"tagged_user_id":54411413,"co_author_invite_id":1723466,"email":"r***l@gmail.com","affiliation":"Nanaji Deshmukh Veterinary Science University. Jabalpur","display_order":6291456,"name":"Rakhi Gangil","title":"Single-chain fragment variable antibody against the capsid protein of bovine immunodeficiency virus and its use in ELISA"},{"id":7717057,"work_id":17119378,"tagging_user_id":36693995,"tagged_user_id":2395666,"co_author_invite_id":null,"email":"d***4@gmail.com","affiliation":"O.P. Jindal Global University","display_order":7340032,"name":"Devyani Gupta","title":"Single-chain fragment variable antibody against the capsid protein of bovine immunodeficiency virus and its use in ELISA"},{"id":7717058,"work_id":17119378,"tagging_user_id":36693995,"tagged_user_id":18520014,"co_author_invite_id":null,"email":"s***6@gmail.com","affiliation":"Banaras Hindu University, Varanasi","display_order":7864320,"name":"SANDEEP KUMAR DUBEY","title":"Single-chain fragment variable antibody against the capsid protein of bovine immunodeficiency virus and its use in ELISA"}],"downloadable_attachments":[],"slug":"Single_chain_fragment_variable_antibody_against_the_capsid_protein_of_bovine_immunodeficiency_virus_and_its_use_in_ELISA","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":36693995,"first_name":"Hare","middle_initials":"","last_name":"Pradhan","page_name":"HarePradhan","domain_name":"independent","created_at":"2015-10-21T11:07:05.415-07:00","display_name":"Hare Pradhan","url":"https://independent.academia.edu/HarePradhan"},"attachments":[],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology"},{"id":6947,"name":"Medical Microbiology","url":"https://www.academia.edu/Documents/in/Medical_Microbiology"},{"id":8089,"name":"Virology","url":"https://www.academia.edu/Documents/in/Virology"},{"id":43838,"name":"India","url":"https://www.academia.edu/Documents/in/India"},{"id":130513,"name":"Phage display","url":"https://www.academia.edu/Documents/in/Phage_display"},{"id":216801,"name":"Journal of Virological Methods","url":"https://www.academia.edu/Documents/in/Journal_of_Virological_Methods"},{"id":260829,"name":"Cattle","url":"https://www.academia.edu/Documents/in/Cattle"},{"id":462111,"name":"Western blot","url":"https://www.academia.edu/Documents/in/Western_blot"},{"id":766014,"name":"Monoclonal Antibody","url":"https://www.academia.edu/Documents/in/Monoclonal_Antibody"},{"id":901876,"name":"Sensitivity and Specificity","url":"https://www.academia.edu/Documents/in/Sensitivity_and_Specificity"},{"id":1248637,"name":"Capsid Protein","url":"https://www.academia.edu/Documents/in/Capsid_Protein"},{"id":1272906,"name":"Enzyme Linked Immunosorbent Assay","url":"https://www.academia.edu/Documents/in/Enzyme_Linked_Immunosorbent_Assay"},{"id":1793091,"name":"Buffaloes","url":"https://www.academia.edu/Documents/in/Buffaloes"},{"id":2256666,"name":"DNA fragmentation","url":"https://www.academia.edu/Documents/in/DNA_fragmentation"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="17119377"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/17119377/Development_of_a_capsid_based_competitive_inhibition_enzyme_linked_immunosorbent_assay_for_detection_of_bovine_immunodeficiency_virus_antibodies_in_cattle_and_buffalo_serum"><img alt="Research paper thumbnail of Development of a capsid based competitive inhibition enzyme-linked immunosorbent assay for detection of bovine immunodeficiency virus antibodies in cattle and buffalo serum" class="work-thumbnail" src="https://attachments.academia-assets.com/42316771/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/17119377/Development_of_a_capsid_based_competitive_inhibition_enzyme_linked_immunosorbent_assay_for_detection_of_bovine_immunodeficiency_virus_antibodies_in_cattle_and_buffalo_serum">Development of a capsid based competitive inhibition enzyme-linked immunosorbent assay for detection of bovine immunodeficiency virus antibodies in cattle and buffalo serum</a></div><div class="wp-workCard_item"><span>Journal of Virological Methods</span><span>, 2008</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="8cc3f5e855d86d56b6e076a53d992dee" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":42316771,"asset_id":17119377,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/42316771/download_file?st=MTczMzE5NzgyMSw4LjIyMi4yMDguMTQ2&st=MTczMzE5NzgyMSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="17119377"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="17119377"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 17119377; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=17119377]").text(description); $(".js-view-count[data-work-id=17119377]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 17119377; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='17119377']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 17119377, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "8cc3f5e855d86d56b6e076a53d992dee" } } $('.js-work-strip[data-work-id=17119377]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":17119377,"title":"Development of a capsid based competitive inhibition enzyme-linked immunosorbent assay for detection of bovine immunodeficiency virus antibodies in cattle and buffalo serum","translated_title":"","metadata":{"grobid_abstract":"The aim of this study was to develop a more specific and sensitive competitive inhibition ELISA (CI-ELISA) than the currently used indirect ELISA for detection of antibodies to bovine immunodeficiency virus (BIV) in cattle and buffaloes. Murine monoclonal antibodies (MAbs) were generated against a recombinant capsid (CA) protein of bovine immunodeficiency virus. Of the 13 anti-CA MAbs developed, MAb-9G10 was selected for CI-ELISA based on the maximum inhibition (98%) obtained with reference BIV antibody positive serum. Based on the distribution of percent inhibition of known negative sera (n = 50), a cut-off value was set at 40% inhibition. The MAb-based CI-ELISA showed much higher agreement (concordance: 95.4%) than the indirect ELISA (concordance: 77.8%) with Western blot. Out of 672 sera of cattle and buffaloes tested by CI-ELISA from four states of India, 22% (113/516) of cattle and 19% (30/156) of buffalo were sero-positive for BIV with an overall seroprevalence of 21% (143/672) in India.","publication_date":{"day":null,"month":null,"year":2008,"errors":{}},"publication_name":"Journal of Virological Methods","grobid_abstract_attachment_id":42316771},"translated_abstract":null,"internal_url":"https://www.academia.edu/17119377/Development_of_a_capsid_based_competitive_inhibition_enzyme_linked_immunosorbent_assay_for_detection_of_bovine_immunodeficiency_virus_antibodies_in_cattle_and_buffalo_serum","translated_internal_url":"","created_at":"2015-10-21T11:12:53.966-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":36693995,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":7717018,"work_id":17119377,"tagging_user_id":36693995,"tagged_user_id":null,"co_author_invite_id":124581,"email":"p***b@gmail.com","display_order":0,"name":"B. Pattnaik","title":"Development of a capsid based competitive inhibition enzyme-linked immunosorbent assay for detection of bovine immunodeficiency virus antibodies in cattle and buffalo serum"},{"id":7717048,"work_id":17119377,"tagging_user_id":36693995,"tagged_user_id":null,"co_author_invite_id":1723465,"email":"b***n@yahoo.co.in","display_order":4194304,"name":"S. Bhatia","title":"Development of a capsid based competitive inhibition enzyme-linked immunosorbent assay for detection of bovine immunodeficiency virus antibodies in cattle and buffalo serum"},{"id":7717051,"work_id":17119377,"tagging_user_id":36693995,"tagged_user_id":29844908,"co_author_invite_id":null,"email":"r***d@icar.gov.in","display_order":6291456,"name":"Richa Sood","title":"Development of a capsid based competitive inhibition enzyme-linked immunosorbent assay for detection of bovine immunodeficiency virus antibodies in cattle and buffalo serum"},{"id":7717055,"work_id":17119377,"tagging_user_id":36693995,"tagged_user_id":5700610,"co_author_invite_id":null,"email":"a***3@yahoo.com","display_order":7340032,"name":"Ashok Bhatia","title":"Development of a capsid based competitive inhibition enzyme-linked immunosorbent assay for detection of bovine immunodeficiency virus antibodies in cattle and buffalo serum"}],"downloadable_attachments":[{"id":42316771,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/42316771/thumbnails/1.jpg","file_name":"Development_of_a_capsid_based_competitiv20160207-11015-mky6he.pdf","download_url":"https://www.academia.edu/attachments/42316771/download_file?st=MTczMzE5NzgyMSw4LjIyMi4yMDguMTQ2&st=MTczMzE5NzgyMSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Development_of_a_capsid_based_competitiv.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/42316771/Development_of_a_capsid_based_competitiv20160207-11015-mky6he-libre.pdf?1454868700=\u0026response-content-disposition=attachment%3B+filename%3DDevelopment_of_a_capsid_based_competitiv.pdf\u0026Expires=1733201421\u0026Signature=LdMqk6EE~JLbsQhnDUrE1pxJuB4y9192HCqQA9ABEJRfOk9RgZGslc1k1S9OQ0dlDqT6vvanSJIGNcvAAoORcDEC0gHciUTfdpbpct--UdgsV947C7d9UTcZ9PQmLo-HXe8LaR2VA80MCRgfmhNyr7JGEhH~pLM9NVwEontIu67sUiU~jGt8S8bgAR5Q6LM-Y8cXnpdtnRrrofNQV6t0dyfj4JsZQdLJFWkUIiQweATjDHVXBh9qgcOhxUToG1Rhf0RtycPynZrR6Kv95E79gtBJam5Jr52PeAU1SMc1LcCrb4r9uNDb8WzJrJ9seko7pZF6DcxvxIpA7mmmFgdBfA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Development_of_a_capsid_based_competitive_inhibition_enzyme_linked_immunosorbent_assay_for_detection_of_bovine_immunodeficiency_virus_antibodies_in_cattle_and_buffalo_serum","translated_slug":"","page_count":8,"language":"en","content_type":"Work","owner":{"id":36693995,"first_name":"Hare","middle_initials":"","last_name":"Pradhan","page_name":"HarePradhan","domain_name":"independent","created_at":"2015-10-21T11:07:05.415-07:00","display_name":"Hare Pradhan","url":"https://independent.academia.edu/HarePradhan"},"attachments":[{"id":42316771,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/42316771/thumbnails/1.jpg","file_name":"Development_of_a_capsid_based_competitiv20160207-11015-mky6he.pdf","download_url":"https://www.academia.edu/attachments/42316771/download_file?st=MTczMzE5NzgyMSw4LjIyMi4yMDguMTQ2&st=MTczMzE5NzgyMSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Development_of_a_capsid_based_competitiv.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/42316771/Development_of_a_capsid_based_competitiv20160207-11015-mky6he-libre.pdf?1454868700=\u0026response-content-disposition=attachment%3B+filename%3DDevelopment_of_a_capsid_based_competitiv.pdf\u0026Expires=1733201421\u0026Signature=LdMqk6EE~JLbsQhnDUrE1pxJuB4y9192HCqQA9ABEJRfOk9RgZGslc1k1S9OQ0dlDqT6vvanSJIGNcvAAoORcDEC0gHciUTfdpbpct--UdgsV947C7d9UTcZ9PQmLo-HXe8LaR2VA80MCRgfmhNyr7JGEhH~pLM9NVwEontIu67sUiU~jGt8S8bgAR5Q6LM-Y8cXnpdtnRrrofNQV6t0dyfj4JsZQdLJFWkUIiQweATjDHVXBh9qgcOhxUToG1Rhf0RtycPynZrR6Kv95E79gtBJam5Jr52PeAU1SMc1LcCrb4r9uNDb8WzJrJ9seko7pZF6DcxvxIpA7mmmFgdBfA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology"},{"id":6947,"name":"Medical Microbiology","url":"https://www.academia.edu/Documents/in/Medical_Microbiology"},{"id":37834,"name":"Western blotting","url":"https://www.academia.edu/Documents/in/Western_blotting"},{"id":43838,"name":"India","url":"https://www.academia.edu/Documents/in/India"},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice"},{"id":147196,"name":"Monoclonal Antibodies","url":"https://www.academia.edu/Documents/in/Monoclonal_Antibodies"},{"id":216801,"name":"Journal of Virological Methods","url":"https://www.academia.edu/Documents/in/Journal_of_Virological_Methods"},{"id":260829,"name":"Cattle","url":"https://www.academia.edu/Documents/in/Cattle"},{"id":462111,"name":"Western blot","url":"https://www.academia.edu/Documents/in/Western_blot"},{"id":766014,"name":"Monoclonal Antibody","url":"https://www.academia.edu/Documents/in/Monoclonal_Antibody"},{"id":1272906,"name":"Enzyme Linked Immunosorbent Assay","url":"https://www.academia.edu/Documents/in/Enzyme_Linked_Immunosorbent_Assay"},{"id":1793091,"name":"Buffaloes","url":"https://www.academia.edu/Documents/in/Buffaloes"}],"urls":[{"id":6522419,"url":"https://www.researchgate.net/profile/Sandeep_Bhatia3/publication/5659596_Development_of_a_capsid_based_competitive_inhibition_enzyme-linked_immunosorbent_assay_for_detection_of_bovine_immunodeficiency_virus_antibodies_in_cattle_and_buffalo_serum/links/0deec52972f6b52c50000000.pdf"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="17119376"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/17119376/Development_and_evaluation_of_SYBR_Green_I_based_one_step_real_time_RT_PCR_assay_for_detection_and_quantification_of_Chikungunya_virus"><img alt="Research paper thumbnail of Development and evaluation of SYBR Green I-based one-step real-time RT-PCR assay for detection and quantification of Chikungunya virus" class="work-thumbnail" src="https://attachments.academia-assets.com/42316777/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/17119376/Development_and_evaluation_of_SYBR_Green_I_based_one_step_real_time_RT_PCR_assay_for_detection_and_quantification_of_Chikungunya_virus">Development and evaluation of SYBR Green I-based one-step real-time RT-PCR assay for detection and quantification of Chikungunya virus</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/HarePradhan">Hare Pradhan</a> and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/PVLakshmanaRao">P. V. Lakshmana Rao</a></span></div><div class="wp-workCard_item"><span>Journal of Clinical Virology</span><span>, 2007</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="95865b56f50a494e3e27f7884e5cd224" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":42316777,"asset_id":17119376,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/42316777/download_file?st=MTczMzE5NzgyMSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="17119376"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="17119376"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 17119376; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=17119376]").text(description); $(".js-view-count[data-work-id=17119376]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 17119376; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='17119376']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 17119376, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "95865b56f50a494e3e27f7884e5cd224" } } $('.js-work-strip[data-work-id=17119376]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":17119376,"title":"Development and evaluation of SYBR Green I-based one-step real-time RT-PCR assay for detection and quantification of Chikungunya virus","translated_title":"","metadata":{"grobid_abstract":"The development of a one-step SYBR Green I-based real-time RT-PCR assay is reported for detection and quantification of Chikungunya virus (CHIKV) in acute-phase patient serum samples by targeting the E1 structural gene. A linear relationship was obtained between the virus concentration and cycle threshold (C t ) value over a range of 10 7 -0.1 PFU/ml. The reported assay was found to be 10-fold more sensitive compared to conventional RT-PCR with a detection limit of 0.1 PFU/ml. The feasibility of this reported assay system for clinical diagnosis was validated with 51 suspected acute-phase serum samples of the recent CHIKV epidemic in southern India, 2006. The comparative evaluation with acute-phase patient serum samples revealed the higher sensitivity of real-time RT-PCR assay by picking up six additional samples with low copy number of template. None of the healthy serum samples analyzed in this study showed amplification. The quantification of the viral load in the acute-phase serum samples was also determined employing the standard curve, which varies from 0.1 to 10 7 PFU/ml. These findings demonstrated that the reported assay has the potential usefulness for clinical diagnosis due to simultaneous detection and quantification of Chikungunya virus in acute-phase patient serum samples.","publication_date":{"day":null,"month":null,"year":2007,"errors":{}},"publication_name":"Journal of Clinical Virology","grobid_abstract_attachment_id":42316777},"translated_abstract":null,"internal_url":"https://www.academia.edu/17119376/Development_and_evaluation_of_SYBR_Green_I_based_one_step_real_time_RT_PCR_assay_for_detection_and_quantification_of_Chikungunya_virus","translated_internal_url":"","created_at":"2015-10-21T11:12:53.855-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":36693995,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":7717020,"work_id":17119376,"tagging_user_id":36693995,"tagged_user_id":null,"co_author_invite_id":124581,"email":"p***b@gmail.com","display_order":0,"name":"B. Pattnaik","title":"Development and evaluation of SYBR Green I-based one-step real-time RT-PCR assay for detection and quantification of Chikungunya virus"},{"id":7717039,"work_id":17119376,"tagging_user_id":36693995,"tagged_user_id":18540878,"co_author_invite_id":null,"email":"s***h@gmail.com","display_order":4194304,"name":"Santhosh Sundaresan","title":"Development and evaluation of SYBR Green I-based one-step real-time RT-PCR assay for detection and quantification of Chikungunya virus"},{"id":7717040,"work_id":17119376,"tagging_user_id":36693995,"tagged_user_id":null,"co_author_invite_id":688053,"email":"p***m@rediffmail.com","display_order":6291456,"name":"M. Parida","title":"Development and evaluation of SYBR Green I-based one-step real-time RT-PCR assay for detection and quantification of Chikungunya virus"},{"id":7717041,"work_id":17119376,"tagging_user_id":36693995,"tagged_user_id":null,"co_author_invite_id":56134,"email":"p***h@rediffmail.com","display_order":7340032,"name":"P. Dash","title":"Development and evaluation of SYBR Green I-based one-step real-time RT-PCR assay for detection and quantification of Chikungunya virus"},{"id":7717042,"work_id":17119376,"tagging_user_id":36693995,"tagged_user_id":61485251,"co_author_invite_id":1723462,"email":"a***a@gmail.com","display_order":7864320,"name":"atul pateriya","title":"Development and evaluation of SYBR Green I-based one-step real-time RT-PCR assay for detection and quantification of Chikungunya virus"},{"id":7717043,"work_id":17119376,"tagging_user_id":36693995,"tagged_user_id":null,"co_author_invite_id":1723463,"email":"a***p@gmail.com","display_order":8126464,"name":"S. Ambuj","title":"Development and evaluation of SYBR Green I-based one-step real-time RT-PCR assay for detection and quantification of Chikungunya virus"},{"id":7717044,"work_id":17119376,"tagging_user_id":36693995,"tagged_user_id":null,"co_author_invite_id":1530203,"email":"n***a@crc.jussieu.fr","display_order":8257536,"name":"N. Gupta","title":"Development and evaluation of SYBR Green I-based one-step real-time RT-PCR assay for detection and quantification of Chikungunya virus"},{"id":7717045,"work_id":17119376,"tagging_user_id":36693995,"tagged_user_id":27069456,"co_author_invite_id":null,"email":"p***a@gmail.com","display_order":8323072,"name":"praveen saxena","title":"Development and evaluation of SYBR Green I-based one-step real-time RT-PCR assay for detection and quantification of Chikungunya virus"},{"id":7717046,"work_id":17119376,"tagging_user_id":36693995,"tagged_user_id":1389695,"co_author_invite_id":null,"email":"p***1@gmail.com","affiliation":"National Institute of Technology, Warangal","display_order":8355840,"name":"Vengal Rao Pachava","title":"Development and evaluation of SYBR Green I-based one-step real-time RT-PCR assay for detection and quantification of Chikungunya virus"},{"id":7717047,"work_id":17119376,"tagging_user_id":36693995,"tagged_user_id":38024303,"co_author_invite_id":1723464,"email":"p***o@rediffmail.com","display_order":8372224,"name":"P. V. Lakshmana Rao","title":"Development and evaluation of SYBR Green I-based one-step real-time RT-PCR assay for detection and quantification of Chikungunya virus"}],"downloadable_attachments":[{"id":42316777,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/42316777/thumbnails/1.jpg","file_name":"j.jcv.2007.04.015.pdf20160207-9459-1rrgo77","download_url":"https://www.academia.edu/attachments/42316777/download_file?st=MTczMzE5NzgyMSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Development_and_evaluation_of_SYBR_Green.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/42316777/j.jcv.2007.04.015-libre.pdf20160207-9459-1rrgo77?1454868700=\u0026response-content-disposition=attachment%3B+filename%3DDevelopment_and_evaluation_of_SYBR_Green.pdf\u0026Expires=1733170423\u0026Signature=Icopv9i-rJ8yP8xu~~dzINVAYmXn2DUV8CRwCJ~Rklu0vApOJw70XWdiWg~sKv1GEOIQA4u6OtjjKdCD0cuGlC1fGm9zHjMqkMDrSpOtaNr6dLvY0EvsNYgXOYoe-g0Vy~imo8y-BV0vQUdz5u2HEnQzcb8gizaz9btDCniPenPFzo9jYPCKNWgJKMz6Wl9AhahUUen7r0~Iq0oFHyyFoGlNyxz-cqCKcqnNnepUoTRx3RrYu6lIQ8OqezQU-CKD5RZhkXNlt2ImrAD7Z1LGu6okRig2E7ZQdPo0Zl2Te5xXPKDKW8ylg-y5so~GPOKuuETGI9XjouJAGPF8UscKNQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Development_and_evaluation_of_SYBR_Green_I_based_one_step_real_time_RT_PCR_assay_for_detection_and_quantification_of_Chikungunya_virus","translated_slug":"","page_count":6,"language":"en","content_type":"Work","owner":{"id":36693995,"first_name":"Hare","middle_initials":"","last_name":"Pradhan","page_name":"HarePradhan","domain_name":"independent","created_at":"2015-10-21T11:07:05.415-07:00","display_name":"Hare Pradhan","url":"https://independent.academia.edu/HarePradhan"},"attachments":[{"id":42316777,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/42316777/thumbnails/1.jpg","file_name":"j.jcv.2007.04.015.pdf20160207-9459-1rrgo77","download_url":"https://www.academia.edu/attachments/42316777/download_file?st=MTczMzE5NzgyMSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Development_and_evaluation_of_SYBR_Green.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/42316777/j.jcv.2007.04.015-libre.pdf20160207-9459-1rrgo77?1454868700=\u0026response-content-disposition=attachment%3B+filename%3DDevelopment_and_evaluation_of_SYBR_Green.pdf\u0026Expires=1733170423\u0026Signature=Icopv9i-rJ8yP8xu~~dzINVAYmXn2DUV8CRwCJ~Rklu0vApOJw70XWdiWg~sKv1GEOIQA4u6OtjjKdCD0cuGlC1fGm9zHjMqkMDrSpOtaNr6dLvY0EvsNYgXOYoe-g0Vy~imo8y-BV0vQUdz5u2HEnQzcb8gizaz9btDCniPenPFzo9jYPCKNWgJKMz6Wl9AhahUUen7r0~Iq0oFHyyFoGlNyxz-cqCKcqnNnepUoTRx3RrYu6lIQ8OqezQU-CKD5RZhkXNlt2ImrAD7Z1LGu6okRig2E7ZQdPo0Zl2Te5xXPKDKW8ylg-y5so~GPOKuuETGI9XjouJAGPF8UscKNQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology"},{"id":6947,"name":"Medical Microbiology","url":"https://www.academia.edu/Documents/in/Medical_Microbiology"},{"id":18529,"name":"Fluorescent Dyes and Reagents","url":"https://www.academia.edu/Documents/in/Fluorescent_Dyes_and_Reagents"},{"id":37833,"name":"Real-time RT-PCR","url":"https://www.academia.edu/Documents/in/Real-time_RT-PCR"},{"id":43838,"name":"India","url":"https://www.academia.edu/Documents/in/India"},{"id":49785,"name":"Clinical Virology","url":"https://www.academia.edu/Documents/in/Clinical_Virology"},{"id":51784,"name":"real time PCR","url":"https://www.academia.edu/Documents/in/real_time_PCR"},{"id":99708,"name":"Clinical","url":"https://www.academia.edu/Documents/in/Clinical"},{"id":210074,"name":"Japanese encephalitis","url":"https://www.academia.edu/Documents/in/Japanese_encephalitis"},{"id":216801,"name":"Journal of Virological Methods","url":"https://www.academia.edu/Documents/in/Journal_of_Virological_Methods"},{"id":229390,"name":"Real Time","url":"https://www.academia.edu/Documents/in/Real_Time"},{"id":244814,"name":"Clinical Sciences","url":"https://www.academia.edu/Documents/in/Clinical_Sciences"},{"id":709507,"name":"Diagnostic Test","url":"https://www.academia.edu/Documents/in/Diagnostic_Test"},{"id":784076,"name":"Species Specificity","url":"https://www.academia.edu/Documents/in/Species_Specificity"},{"id":877165,"name":"Chikungunya Virus","url":"https://www.academia.edu/Documents/in/Chikungunya_Virus"},{"id":901876,"name":"Sensitivity and Specificity","url":"https://www.academia.edu/Documents/in/Sensitivity_and_Specificity"},{"id":1193376,"name":"Japanese Encephalitis Virus","url":"https://www.academia.edu/Documents/in/Japanese_Encephalitis_Virus"},{"id":1275886,"name":"Clinical Application","url":"https://www.academia.edu/Documents/in/Clinical_Application"},{"id":1297608,"name":"Organic Chemicals","url":"https://www.academia.edu/Documents/in/Organic_Chemicals"},{"id":1434202,"name":"Clinical Diagnosis","url":"https://www.academia.edu/Documents/in/Clinical_Diagnosis"},{"id":2249317,"name":"Viral Load","url":"https://www.academia.edu/Documents/in/Viral_Load"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="17119375"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/17119375/Genetic_Analysis_and_Expression_of_NS3_Gene_of_Bovine_Viral_Diarrhoea_Virus_1_from_India_for_Detection_of_Antibodies_in_Cattle"><img alt="Research paper thumbnail of Genetic Analysis and Expression of NS3 Gene of Bovine Viral Diarrhoea Virus 1 from India for Detection of Antibodies in Cattle" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/17119375/Genetic_Analysis_and_Expression_of_NS3_Gene_of_Bovine_Viral_Diarrhoea_Virus_1_from_India_for_Detection_of_Antibodies_in_Cattle">Genetic Analysis and Expression of NS3 Gene of Bovine Viral Diarrhoea Virus 1 from India for Detection of Antibodies in Cattle</a></div><div class="wp-workCard_item"><span>Journal of Applied Animal Research</span><span>, 2008</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Mishra, N., Pitale, S.S. and Pradhan, H.K. 2008. Genetic analysis and expression of NS3 gene of b...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Mishra, N., Pitale, S.S. and Pradhan, H.K. 2008. Genetic analysis and expression of NS3 gene of bovine viral diarrhoea virus 1 from India for detection of antibodies in cattle. J. Appl. Anim. Res., 33: 99–103.Considering the importance of NS3 antigen in diagnosis of bovine viral diarrhea virus (BVDV) infection, we analysed genetically selected Indian isolates in NS3 gene region and</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="17119375"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="17119375"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 17119375; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=17119375]").text(description); $(".js-view-count[data-work-id=17119375]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 17119375; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='17119375']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 17119375, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=17119375]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":17119375,"title":"Genetic Analysis and Expression of NS3 Gene of Bovine Viral Diarrhoea Virus 1 from India for Detection of Antibodies in Cattle","translated_title":"","metadata":{"abstract":"Mishra, N., Pitale, S.S. and Pradhan, H.K. 2008. Genetic analysis and expression of NS3 gene of bovine viral diarrhoea virus 1 from India for detection of antibodies in cattle. J. Appl. Anim. Res., 33: 99–103.Considering the importance of NS3 antigen in diagnosis of bovine viral diarrhea virus (BVDV) infection, we analysed genetically selected Indian isolates in NS3 gene region and","publication_date":{"day":null,"month":null,"year":2008,"errors":{}},"publication_name":"Journal of Applied Animal Research"},"translated_abstract":"Mishra, N., Pitale, S.S. and Pradhan, H.K. 2008. Genetic analysis and expression of NS3 gene of bovine viral diarrhoea virus 1 from India for detection of antibodies in cattle. J. Appl. Anim. Res., 33: 99–103.Considering the importance of NS3 antigen in diagnosis of bovine viral diarrhea virus (BVDV) infection, we analysed genetically selected Indian isolates in NS3 gene region and","internal_url":"https://www.academia.edu/17119375/Genetic_Analysis_and_Expression_of_NS3_Gene_of_Bovine_Viral_Diarrhoea_Virus_1_from_India_for_Detection_of_Antibodies_in_Cattle","translated_internal_url":"","created_at":"2015-10-21T11:12:53.732-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":36693995,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":7717031,"work_id":17119375,"tagging_user_id":36693995,"tagged_user_id":50823026,"co_author_invite_id":616470,"email":"m***r@rediffmail.com","display_order":0,"name":"Niranjan Mishra","title":"Genetic Analysis and Expression of NS3 Gene of Bovine Viral Diarrhoea Virus 1 from India for Detection of Antibodies in Cattle"},{"id":7717036,"work_id":17119375,"tagging_user_id":36693995,"tagged_user_id":1175172,"co_author_invite_id":null,"email":"s***9@gmail.com","display_order":4194304,"name":"Shreyas Pitale","title":"Genetic Analysis and Expression of NS3 Gene of Bovine Viral Diarrhoea Virus 1 from India for Detection of Antibodies in Cattle"}],"downloadable_attachments":[],"slug":"Genetic_Analysis_and_Expression_of_NS3_Gene_of_Bovine_Viral_Diarrhoea_Virus_1_from_India_for_Detection_of_Antibodies_in_Cattle","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":36693995,"first_name":"Hare","middle_initials":"","last_name":"Pradhan","page_name":"HarePradhan","domain_name":"independent","created_at":"2015-10-21T11:07:05.415-07:00","display_name":"Hare Pradhan","url":"https://independent.academia.edu/HarePradhan"},"attachments":[],"research_interests":[{"id":173,"name":"Zoology","url":"https://www.academia.edu/Documents/in/Zoology"},{"id":29980,"name":"Animal Production","url":"https://www.academia.edu/Documents/in/Animal_Production"},{"id":38884,"name":"Applied Animal Behavior","url":"https://www.academia.edu/Documents/in/Applied_Animal_Behavior"},{"id":83128,"name":"Escherichia coli","url":"https://www.academia.edu/Documents/in/Escherichia_coli"},{"id":284067,"name":"Inclusion Bodies","url":"https://www.academia.edu/Documents/in/Inclusion_Bodies"},{"id":462111,"name":"Western blot","url":"https://www.academia.edu/Documents/in/Western_blot"},{"id":707213,"name":"Genetic Analysis","url":"https://www.academia.edu/Documents/in/Genetic_Analysis"},{"id":809881,"name":"Amino Acid Sequence","url":"https://www.academia.edu/Documents/in/Amino_Acid_Sequence"},{"id":959510,"name":"Recombinant Protein","url":"https://www.academia.edu/Documents/in/Recombinant_Protein"},{"id":1387364,"name":"Genetic Selection","url":"https://www.academia.edu/Documents/in/Genetic_Selection"},{"id":1597877,"name":"Nucleotides","url":"https://www.academia.edu/Documents/in/Nucleotides"},{"id":1709309,"name":"Bovine Viral Diarrhea Virus","url":"https://www.academia.edu/Documents/in/Bovine_Viral_Diarrhea_Virus"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="17119374"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/17119374/Rapid_Detection_of_Highly_Pathogenic_Avian_Influenza_H5N1_Virus_by_TaqMan_Reverse_Transcriptase_Polymerase_Chain_Reaction"><img alt="Research paper thumbnail of Rapid Detection of Highly Pathogenic Avian Influenza H5N1 Virus by TaqMan Reverse Transcriptase–Polymerase Chain Reaction" class="work-thumbnail" src="https://attachments.academia-assets.com/42316756/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/17119374/Rapid_Detection_of_Highly_Pathogenic_Avian_Influenza_H5N1_Virus_by_TaqMan_Reverse_Transcriptase_Polymerase_Chain_Reaction">Rapid Detection of Highly Pathogenic Avian Influenza H5N1 Virus by TaqMan Reverse Transcriptase–Polymerase Chain Reaction</a></div><div class="wp-workCard_item"><span>Avian Diseases</span><span>, 2007</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="d433cfaffdfbe1178853a229f15b800e" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":42316756,"asset_id":17119374,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/42316756/download_file?st=MTczMzE5NzgyMSw4LjIyMi4yMDguMTQ2&st=MTczMzE5NzgyMSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="17119374"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="17119374"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 17119374; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=17119374]").text(description); $(".js-view-count[data-work-id=17119374]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 17119374; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='17119374']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 17119374, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "d433cfaffdfbe1178853a229f15b800e" } } $('.js-work-strip[data-work-id=17119374]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":17119374,"title":"Rapid Detection of Highly Pathogenic Avian Influenza H5N1 Virus by TaqMan Reverse Transcriptase–Polymerase Chain Reaction","translated_title":"","metadata":{"grobid_abstract":"Highly pathogenic Avian Influenza (AI) H5N1 viruses have been spreading from Asia since late 2003. Early detection and classification are paramount for control of the disease because these viruses are lethal to birds and have caused fatalities in humans. Here we describe a TaqMan Reverse Transcriptase-Polymerase Chain Reaction Assay for rapid detection of Avian Influenza virus and for H5 subtyping by targeting HA gene of AI viruses. The assay was highly sensitive than RT-PCR and virus isolation in chick embryos. In the present study all samples (field samples) which are positive for HI and RT-PCR were tested by using TaqMan Reverse Transcriptase-Polymerase Chain Reaction Assay for reconfirmation. AI viruses (H5N1) were detected from nine samples which are received from Maharashtra during Avian influenza outbreak in India in 2006. Real-Time PCR assays was also conducted for detection of viral genome in different organs of experimental infected chickens revealed presence of the virus in all organs with high virus concentration in brain, heart, intestine and cloaca. This test allows definitive confirmation of an AI virus as H5 within hours, which is crucial for rapid implementation of control measures in the event of an outbreak.","publication_date":{"day":null,"month":null,"year":2007,"errors":{}},"publication_name":"Avian Diseases","grobid_abstract_attachment_id":42316756},"translated_abstract":null,"internal_url":"https://www.academia.edu/17119374/Rapid_Detection_of_Highly_Pathogenic_Avian_Influenza_H5N1_Virus_by_TaqMan_Reverse_Transcriptase_Polymerase_Chain_Reaction","translated_internal_url":"","created_at":"2015-10-21T11:12:53.624-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":36693995,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":7717017,"work_id":17119374,"tagging_user_id":36693995,"tagged_user_id":null,"co_author_invite_id":1723458,"email":"m***1@gmail.com","display_order":0,"name":"B. Shankar","title":"Rapid Detection of Highly Pathogenic Avian Influenza H5N1 Virus by TaqMan Reverse Transcriptase–Polymerase Chain Reaction"},{"id":7717021,"work_id":17119374,"tagging_user_id":36693995,"tagged_user_id":null,"co_author_invite_id":124581,"email":"p***b@gmail.com","display_order":4194304,"name":"B. Pattnaik","title":"Rapid Detection of Highly Pathogenic Avian Influenza H5N1 Virus by TaqMan Reverse Transcriptase–Polymerase Chain Reaction"},{"id":7717024,"work_id":17119374,"tagging_user_id":36693995,"tagged_user_id":160479623,"co_author_invite_id":1723459,"email":"b***i@gmail.com","display_order":6291456,"name":"BL Manjunath","title":"Rapid Detection of Highly Pathogenic Avian Influenza H5N1 Virus by TaqMan Reverse Transcriptase–Polymerase Chain Reaction"},{"id":7717026,"work_id":17119374,"tagging_user_id":36693995,"tagged_user_id":7223595,"co_author_invite_id":null,"email":"d***l@gmail.com","display_order":7340032,"name":"Suresh Patil","title":"Rapid Detection of Highly Pathogenic Avian Influenza H5N1 Virus by TaqMan Reverse Transcriptase–Polymerase Chain Reaction"},{"id":7717027,"work_id":17119374,"tagging_user_id":36693995,"tagged_user_id":16665419,"co_author_invite_id":null,"email":"b***t@gmail.com","display_order":7864320,"name":"B.Sushant Patnaik","title":"Rapid Detection of Highly Pathogenic Avian Influenza H5N1 Virus by TaqMan Reverse Transcriptase–Polymerase Chain Reaction"},{"id":7717029,"work_id":17119374,"tagging_user_id":36693995,"tagged_user_id":null,"co_author_invite_id":1723460,"email":"d***p@gmail.com","display_order":8126464,"name":"Shankar B.p","title":"Rapid Detection of Highly Pathogenic Avian Influenza H5N1 Virus by TaqMan Reverse Transcriptase–Polymerase Chain Reaction"},{"id":7717030,"work_id":17119374,"tagging_user_id":36693995,"tagged_user_id":null,"co_author_invite_id":1723461,"email":"c***d@mit.asia","display_order":8257536,"name":"Patil S.s","title":"Rapid Detection of Highly Pathogenic Avian Influenza H5N1 Virus by TaqMan Reverse Transcriptase–Polymerase Chain Reaction"},{"id":7717100,"work_id":17119374,"tagging_user_id":36693995,"tagged_user_id":null,"co_author_invite_id":1723481,"email":"r***a@yahoo.co.in","display_order":8323072,"name":"Gowda R.n.s","title":"Rapid Detection of Highly Pathogenic Avian Influenza H5N1 Virus by TaqMan Reverse Transcriptase–Polymerase Chain Reaction"},{"id":7717101,"work_id":17119374,"tagging_user_id":36693995,"tagged_user_id":null,"co_author_invite_id":1723482,"email":"p***b@pdfmd.ernet.in","display_order":8355840,"name":"Pattnaik B","title":"Rapid Detection of Highly Pathogenic Avian Influenza H5N1 Virus by TaqMan Reverse Transcriptase–Polymerase Chain Reaction"}],"downloadable_attachments":[{"id":42316756,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/42316756/thumbnails/1.jpg","file_name":"fin1278.pdf","download_url":"https://www.academia.edu/attachments/42316756/download_file?st=MTczMzE5NzgyMSw4LjIyMi4yMDguMTQ2&st=MTczMzE5NzgyMSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Rapid_Detection_of_Highly_Pathogenic_Avi.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/42316756/fin1278-libre.pdf?1454868701=\u0026response-content-disposition=attachment%3B+filename%3DRapid_Detection_of_Highly_Pathogenic_Avi.pdf\u0026Expires=1733201421\u0026Signature=K8loWgGYBQzfjcZuKVVzfdAxfXc329cjeAODdoIl76Qm5juS~s1aS4UP~yxTz57GN8cRBGkp9KizsT~tSU-fKRJrbceZx1M5LrPovVz5qB0omlOZ7NrGmNIiqkn3y9iuJ~3TITjsPWOA72JSMwMtJcCF1O5WNeBodBvY-SMgzipTAXH2rD-MvstZSB7aqh260MRJkAjuGmixgfE95H69dm3WzPQYk~SUWfhcaV4olOBDkmeEOQiys9sbcnsZDa-RLdfMUsg-uCBbE~BIvQhwhFb9388cmVSwpjEZpfMCuD6atu-Xl3AYr2~Alujl3QjTyEra-TClcCycabcOu3~fFw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Rapid_Detection_of_Highly_Pathogenic_Avian_Influenza_H5N1_Virus_by_TaqMan_Reverse_Transcriptase_Polymerase_Chain_Reaction","translated_slug":"","page_count":4,"language":"en","content_type":"Work","owner":{"id":36693995,"first_name":"Hare","middle_initials":"","last_name":"Pradhan","page_name":"HarePradhan","domain_name":"independent","created_at":"2015-10-21T11:07:05.415-07:00","display_name":"Hare Pradhan","url":"https://independent.academia.edu/HarePradhan"},"attachments":[{"id":42316756,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/42316756/thumbnails/1.jpg","file_name":"fin1278.pdf","download_url":"https://www.academia.edu/attachments/42316756/download_file?st=MTczMzE5NzgyMSw4LjIyMi4yMDguMTQ2&st=MTczMzE5NzgyMSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Rapid_Detection_of_Highly_Pathogenic_Avi.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/42316756/fin1278-libre.pdf?1454868701=\u0026response-content-disposition=attachment%3B+filename%3DRapid_Detection_of_Highly_Pathogenic_Avi.pdf\u0026Expires=1733201421\u0026Signature=K8loWgGYBQzfjcZuKVVzfdAxfXc329cjeAODdoIl76Qm5juS~s1aS4UP~yxTz57GN8cRBGkp9KizsT~tSU-fKRJrbceZx1M5LrPovVz5qB0omlOZ7NrGmNIiqkn3y9iuJ~3TITjsPWOA72JSMwMtJcCF1O5WNeBodBvY-SMgzipTAXH2rD-MvstZSB7aqh260MRJkAjuGmixgfE95H69dm3WzPQYk~SUWfhcaV4olOBDkmeEOQiys9sbcnsZDa-RLdfMUsg-uCBbE~BIvQhwhFb9388cmVSwpjEZpfMCuD6atu-Xl3AYr2~Alujl3QjTyEra-TClcCycabcOu3~fFw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology"},{"id":173,"name":"Zoology","url":"https://www.academia.edu/Documents/in/Zoology"},{"id":3092,"name":"Poultry Science","url":"https://www.academia.edu/Documents/in/Poultry_Science"},{"id":51784,"name":"real time PCR","url":"https://www.academia.edu/Documents/in/real_time_PCR"},{"id":83205,"name":"Avian Influenza","url":"https://www.academia.edu/Documents/in/Avian_Influenza"},{"id":96324,"name":"Birds","url":"https://www.academia.edu/Documents/in/Birds"},{"id":238630,"name":"Experimental Infection","url":"https://www.academia.edu/Documents/in/Experimental_Infection"},{"id":371528,"name":"Early Detection","url":"https://www.academia.edu/Documents/in/Early_Detection"},{"id":644860,"name":"Veterinary Sciences","url":"https://www.academia.edu/Documents/in/Veterinary_Sciences"},{"id":770676,"name":"Avian Diseases","url":"https://www.academia.edu/Documents/in/Avian_Diseases"},{"id":2007837,"name":"Virus isolation","url":"https://www.academia.edu/Documents/in/Virus_isolation"},{"id":2029070,"name":"Highly pathogenic avian influenza","url":"https://www.academia.edu/Documents/in/Highly_pathogenic_avian_influenza"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="13998994"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/13998994/Genetic_typing_of_bovine_viral_diarrhoea_virus_isolates_from_India"><img alt="Research paper thumbnail of Genetic typing of bovine viral diarrhoea virus isolates from India" class="work-thumbnail" src="https://attachments.academia-assets.com/44717609/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/13998994/Genetic_typing_of_bovine_viral_diarrhoea_virus_isolates_from_India">Genetic typing of bovine viral diarrhoea virus isolates from India</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/StefanVilcek">Stefan Vilcek</a> and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/HarePradhan">Hare Pradhan</a></span></div><div class="wp-workCard_item"><span>Veterinary Microbiology</span><span>, 2004</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="75ba5e506c7bb5c83239aef01c67d768" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":44717609,"asset_id":13998994,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/44717609/download_file?st=MTczMzE5NzgyMSw4LjIyMi4yMDguMTQ2&st=MTczMzE5NzgyMSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="13998994"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="13998994"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 13998994; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=13998994]").text(description); $(".js-view-count[data-work-id=13998994]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 13998994; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='13998994']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 13998994, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "75ba5e506c7bb5c83239aef01c67d768" } } $('.js-work-strip[data-work-id=13998994]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":13998994,"title":"Genetic typing of bovine viral diarrhoea virus isolates from India","translated_title":"","metadata":{"ai_title_tag":"Genetic Typing of BVDV Isolates Across India","grobid_abstract":"Thirteen BVDV isolates collected in four geographic regions of India between 2000 and 2002 were typed in 5 0 -UTR. To confirm results of genetic typing, selected viruses were also analysed in the N pro region. Phylogenetic analysis revealed that all Indian BVDV isolates belong to BVDV-1b (Osloss-like group). Despite a long distance between the farms from which the viruses were isolated there was no correlation between the origin of viral isolates and their position in a phylogenetic tree. Higher genetic similarity of Indian BVDV isolates was observed most probably due to the uncontrolled movement of cattle as well as the uncontrolled use of semen from bulls for breeding of local and farm cattle in different states of India.","publication_date":{"day":null,"month":null,"year":2004,"errors":{}},"publication_name":"Veterinary Microbiology","grobid_abstract_attachment_id":44717609},"translated_abstract":null,"internal_url":"https://www.academia.edu/13998994/Genetic_typing_of_bovine_viral_diarrhoea_virus_isolates_from_India","translated_internal_url":"","created_at":"2015-07-13T10:43:02.263-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":33035758,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":3051774,"work_id":13998994,"tagging_user_id":33035758,"tagged_user_id":null,"co_author_invite_id":774789,"email":"d***l@gtnet.gov.uk","display_order":0,"name":"D. Paton","title":"Genetic typing of bovine viral diarrhoea virus isolates from India"},{"id":3051800,"work_id":13998994,"tagging_user_id":33035758,"tagged_user_id":57938591,"co_author_invite_id":774792,"email":"w***r@vu-wien.ac.at","display_order":4194304,"name":"W. Baumgartner","title":"Genetic typing of bovine viral diarrhoea virus isolates from India"},{"id":7483474,"work_id":13998994,"tagging_user_id":33035758,"tagged_user_id":null,"co_author_invite_id":1671833,"email":"d***c@vf.uni-lj.si","display_order":6291456,"name":"Darja Barlič-maganja","title":"Genetic typing of bovine viral diarrhoea virus isolates from India"},{"id":7483475,"work_id":13998994,"tagging_user_id":33035758,"tagged_user_id":36693995,"co_author_invite_id":1671834,"email":"h***5@rediffmail.com","display_order":7340032,"name":"Hare Pradhan","title":"Genetic typing of bovine viral diarrhoea virus isolates from India"},{"id":7483476,"work_id":13998994,"tagging_user_id":33035758,"tagged_user_id":20047756,"co_author_invite_id":null,"email":"j***4@gmail.com","display_order":7864320,"name":"Pooja Jain","title":"Genetic typing of bovine viral diarrhoea virus isolates from India"},{"id":7483477,"work_id":13998994,"tagging_user_id":33035758,"tagged_user_id":15560863,"co_author_invite_id":null,"email":"a***1@gmail.com","display_order":8126464,"name":"Pooja Jain","title":"Genetic typing of bovine viral diarrhoea virus isolates from India"},{"id":7483478,"work_id":13998994,"tagging_user_id":33035758,"tagged_user_id":18206113,"co_author_invite_id":null,"email":"j***3@gmail.com","display_order":8257536,"name":"Pooja Jain","title":"Genetic typing of bovine viral diarrhoea virus isolates from India"},{"id":7483479,"work_id":13998994,"tagging_user_id":33035758,"tagged_user_id":16219264,"co_author_invite_id":null,"email":"p***5@gmail.com","display_order":8323072,"name":"pooja jain","title":"Genetic typing of bovine viral diarrhoea virus isolates from India"},{"id":7483480,"work_id":13998994,"tagging_user_id":33035758,"tagged_user_id":12422335,"co_author_invite_id":null,"email":"p***n@gmail.com","display_order":8355840,"name":"pooja jain","title":"Genetic typing of bovine viral diarrhoea virus isolates from India"},{"id":7483481,"work_id":13998994,"tagging_user_id":33035758,"tagged_user_id":11839197,"co_author_invite_id":null,"email":"j***1@yahoo.co.in","display_order":8372224,"name":"POOJA JAIN","title":"Genetic typing of bovine viral diarrhoea virus isolates from India"},{"id":7483482,"work_id":13998994,"tagging_user_id":33035758,"tagged_user_id":null,"co_author_invite_id":750022,"email":"p***n@drexelmed.edu","display_order":8380416,"name":"Pooja Jain","title":"Genetic typing of bovine viral diarrhoea virus isolates from India"},{"id":7483483,"work_id":13998994,"tagging_user_id":33035758,"tagged_user_id":null,"co_author_invite_id":1671835,"email":"t***k@vla.defra.gsi.gov.uk","display_order":8384512,"name":"Torstein Sandvik","title":"Genetic typing of bovine viral diarrhoea virus isolates from India"}],"downloadable_attachments":[{"id":44717609,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/44717609/thumbnails/1.jpg","file_name":"0deec5297319827dcf000000.pdf","download_url":"https://www.academia.edu/attachments/44717609/download_file?st=MTczMzE5NzgyMSw4LjIyMi4yMDguMTQ2&st=MTczMzE5NzgyMSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Genetic_typing_of_bovine_viral_diarrhoea.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/44717609/0deec5297319827dcf000000-libre.pdf?1460615205=\u0026response-content-disposition=attachment%3B+filename%3DGenetic_typing_of_bovine_viral_diarrhoea.pdf\u0026Expires=1733201421\u0026Signature=GMJ1TI4gDUD1o7oMGBKsfceRSX5CN3Ehur2-8U1Y1KffbYtxWePw9eG-WGdiZEkeYnIfxTmzc5AgiBxCWM0h0qeP7F5TJQvC6~hlalk6CJa6C8g4P-lZl6qmwgIJekhMhAiDbZFda5s0pvNW7-A0gA4FafRDWgQkXU5EeJDt5z2BLB3KWCiwZDmXXMDCdiqk7IqqkYY-haaB~XdD4fPxkPHe8luZFoGgYJU5I~L25xk6vlh8n4OLbwzv56uriFKmRkZ8wtQIB1z8f6j2I4QPeGwE6gEK~RJTB9Pm9LeU1Kh6nfh9ktrUmpWZtAeKzGWPpPiGgm2JAKCZxGUKN3s2nA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Genetic_typing_of_bovine_viral_diarrhoea_virus_isolates_from_India","translated_slug":"","page_count":6,"language":"en","content_type":"Work","owner":{"id":33035758,"first_name":"Stefan","middle_initials":null,"last_name":"Vilcek","page_name":"StefanVilcek","domain_name":"independent","created_at":"2015-07-13T10:42:22.347-07:00","display_name":"Stefan Vilcek","url":"https://independent.academia.edu/StefanVilcek"},"attachments":[{"id":44717609,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/44717609/thumbnails/1.jpg","file_name":"0deec5297319827dcf000000.pdf","download_url":"https://www.academia.edu/attachments/44717609/download_file?st=MTczMzE5NzgyMSw4LjIyMi4yMDguMTQ2&st=MTczMzE5NzgyMSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Genetic_typing_of_bovine_viral_diarrhoea.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/44717609/0deec5297319827dcf000000-libre.pdf?1460615205=\u0026response-content-disposition=attachment%3B+filename%3DGenetic_typing_of_bovine_viral_diarrhoea.pdf\u0026Expires=1733201421\u0026Signature=GMJ1TI4gDUD1o7oMGBKsfceRSX5CN3Ehur2-8U1Y1KffbYtxWePw9eG-WGdiZEkeYnIfxTmzc5AgiBxCWM0h0qeP7F5TJQvC6~hlalk6CJa6C8g4P-lZl6qmwgIJekhMhAiDbZFda5s0pvNW7-A0gA4FafRDWgQkXU5EeJDt5z2BLB3KWCiwZDmXXMDCdiqk7IqqkYY-haaB~XdD4fPxkPHe8luZFoGgYJU5I~L25xk6vlh8n4OLbwzv56uriFKmRkZ8wtQIB1z8f6j2I4QPeGwE6gEK~RJTB9Pm9LeU1Kh6nfh9ktrUmpWZtAeKzGWPpPiGgm2JAKCZxGUKN3s2nA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":156,"name":"Genetics","url":"https://www.academia.edu/Documents/in/Genetics"},{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology"},{"id":3037,"name":"Veterinary Microbiology","url":"https://www.academia.edu/Documents/in/Veterinary_Microbiology"},{"id":35414,"name":"Veterinary","url":"https://www.academia.edu/Documents/in/Veterinary"},{"id":37782,"name":"Cell Culture","url":"https://www.academia.edu/Documents/in/Cell_Culture"},{"id":43838,"name":"India","url":"https://www.academia.edu/Documents/in/India"},{"id":54433,"name":"Phylogeny","url":"https://www.academia.edu/Documents/in/Phylogeny"},{"id":67484,"name":"Sequence alignment","url":"https://www.academia.edu/Documents/in/Sequence_alignment"},{"id":158782,"name":"Phylogenetic analysis","url":"https://www.academia.edu/Documents/in/Phylogenetic_analysis"},{"id":178709,"name":"Slovenia","url":"https://www.academia.edu/Documents/in/Slovenia"},{"id":260829,"name":"Cattle","url":"https://www.academia.edu/Documents/in/Cattle"},{"id":372410,"name":"Genotype","url":"https://www.academia.edu/Documents/in/Genotype"},{"id":486784,"name":"Genotyping","url":"https://www.academia.edu/Documents/in/Genotyping"},{"id":550697,"name":"Phylogenetic Tree","url":"https://www.academia.edu/Documents/in/Phylogenetic_Tree"},{"id":577933,"name":"Genetic variation","url":"https://www.academia.edu/Documents/in/Genetic_variation"},{"id":644860,"name":"Veterinary Sciences","url":"https://www.academia.edu/Documents/in/Veterinary_Sciences"},{"id":809882,"name":"Base Sequence","url":"https://www.academia.edu/Documents/in/Base_Sequence"},{"id":964206,"name":"Genetic Similarity","url":"https://www.academia.edu/Documents/in/Genetic_Similarity"},{"id":1714028,"name":"Long Distance","url":"https://www.academia.edu/Documents/in/Long_Distance"},{"id":2007837,"name":"Virus isolation","url":"https://www.academia.edu/Documents/in/Virus_isolation"},{"id":2056385,"name":"Bovine viral diarrhoea","url":"https://www.academia.edu/Documents/in/Bovine_viral_diarrhoea"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> </div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/google_contacts-0dfb882d836b94dbcb4a2d123d6933fc9533eda5be911641f20b4eb428429600.js"], function() { // from javascript_helper.rb $('.js-google-connect-button').click(function(e) { e.preventDefault(); GoogleContacts.authorize_and_show_contacts(); Aedu.Dismissibles.recordClickthrough("WowProfileImportContactsPrompt"); }); $('.js-update-biography-button').click(function(e) { e.preventDefault(); Aedu.Dismissibles.recordClickthrough("UpdateUserBiographyPrompt"); $.ajax({ url: $r.api_v0_profiles_update_about_path({ subdomain_param: 'api', about: "", }), type: 'PUT', success: function(response) { location.reload(); } }); }); $('.js-work-creator-button').click(function (e) { e.preventDefault(); window.location = $r.upload_funnel_document_path({ source: encodeURIComponent(""), }); }); $('.js-video-upload-button').click(function (e) { e.preventDefault(); window.location = $r.upload_funnel_video_path({ source: encodeURIComponent(""), }); }); $('.js-do-this-later-button').click(function() { $(this).closest('.js-profile-nag-panel').remove(); Aedu.Dismissibles.recordDismissal("WowProfileImportContactsPrompt"); }); $('.js-update-biography-do-this-later-button').click(function(){ $(this).closest('.js-profile-nag-panel').remove(); Aedu.Dismissibles.recordDismissal("UpdateUserBiographyPrompt"); }); $('.wow-profile-mentions-upsell--close').click(function(){ $('.wow-profile-mentions-upsell--panel').hide(); Aedu.Dismissibles.recordDismissal("WowProfileMentionsUpsell"); }); $('.wow-profile-mentions-upsell--button').click(function(){ Aedu.Dismissibles.recordClickthrough("WowProfileMentionsUpsell"); }); new WowProfile.SocialRedesignUserWorks({ initialWorksOffset: 20, allWorksOffset: 20, maxSections: 1 }) }); </script> </div></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile_edit-5ea339ee107c863779f560dd7275595239fed73f1a13d279d2b599a28c0ecd33.js","https://a.academia-assets.com/assets/add_coauthor-22174b608f9cb871d03443cafa7feac496fb50d7df2d66a53f5ee3c04ba67f53.js","https://a.academia-assets.com/assets/tab-dcac0130902f0cc2d8cb403714dd47454f11fc6fb0e99ae6a0827b06613abc20.js","https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js"], function() { // from javascript_helper.rb window.ae = window.ae || {}; window.ae.WowProfile = window.ae.WowProfile || {}; if(Aedu.User.current && Aedu.User.current.id === $viewedUser.id) { window.ae.WowProfile.current_user_edit = {}; new WowProfileEdit.EditUploadView({ el: '.js-edit-upload-button-wrapper', model: window.$current_user, }); new AddCoauthor.AddCoauthorsController(); } var userInfoView = new WowProfile.SocialRedesignUserInfo({ recaptcha_key: "6LdxlRMTAAAAADnu_zyLhLg0YF9uACwz78shpjJB" }); WowProfile.router = new WowProfile.Router({ userInfoView: userInfoView }); Backbone.history.start({ pushState: true, root: "/" + $viewedUser.page_name }); new WowProfile.UserWorksNav() }); </script> </div> <div class="bootstrap login"><div class="modal fade login-modal" id="login-modal"><div class="login-modal-dialog modal-dialog"><div class="modal-content"><div class="modal-header"><button class="close close" data-dismiss="modal" type="button"><span aria-hidden="true">×</span><span class="sr-only">Close</span></button><h4 class="modal-title text-center"><strong>Log In</strong></h4></div><div class="modal-body"><div class="row"><div class="col-xs-10 col-xs-offset-1"><button class="btn btn-fb btn-lg btn-block btn-v-center-content" id="login-facebook-oauth-button"><svg style="float: left; width: 19px; line-height: 1em; margin-right: .3em;" aria-hidden="true" focusable="false" data-prefix="fab" data-icon="facebook-square" class="svg-inline--fa fa-facebook-square fa-w-14" role="img" xmlns="http://www.w3.org/2000/svg" viewBox="0 0 448 512"><path fill="currentColor" d="M400 32H48A48 48 0 0 0 0 80v352a48 48 0 0 0 48 48h137.25V327.69h-63V256h63v-54.64c0-62.15 37-96.48 93.67-96.48 27.14 0 55.52 4.84 55.52 4.84v61h-31.27c-30.81 0-40.42 19.12-40.42 38.73V256h68.78l-11 71.69h-57.78V480H400a48 48 0 0 0 48-48V80a48 48 0 0 0-48-48z"></path></svg><small><strong>Log in</strong> with <strong>Facebook</strong></small></button><br /><button class="btn btn-google btn-lg btn-block btn-v-center-content" id="login-google-oauth-button"><svg style="float: left; width: 22px; line-height: 1em; margin-right: .3em;" aria-hidden="true" focusable="false" data-prefix="fab" data-icon="google-plus" class="svg-inline--fa fa-google-plus fa-w-16" role="img" xmlns="http://www.w3.org/2000/svg" viewBox="0 0 512 512"><path fill="currentColor" d="M256,8C119.1,8,8,119.1,8,256S119.1,504,256,504,504,392.9,504,256,392.9,8,256,8ZM185.3,380a124,124,0,0,1,0-248c31.3,0,60.1,11,83,32.3l-33.6,32.6c-13.2-12.9-31.3-19.1-49.4-19.1-42.9,0-77.2,35.5-77.2,78.1S142.3,334,185.3,334c32.6,0,64.9-19.1,70.1-53.3H185.3V238.1H302.2a109.2,109.2,0,0,1,1.9,20.7c0,70.8-47.5,121.2-118.8,121.2ZM415.5,273.8v35.5H380V273.8H344.5V238.3H380V202.8h35.5v35.5h35.2v35.5Z"></path></svg><small><strong>Log in</strong> with <strong>Google</strong></small></button><br /><style type="text/css">.sign-in-with-apple-button { width: 100%; height: 52px; border-radius: 3px; border: 1px solid black; cursor: pointer; }</style><script src="https://appleid.cdn-apple.com/appleauth/static/jsapi/appleid/1/en_US/appleid.auth.js" type="text/javascript"></script><div class="sign-in-with-apple-button" data-border="false" data-color="white" id="appleid-signin"><span   ="Sign Up with Apple" class="u-fs11"></span></div><script>AppleID.auth.init({ clientId: 'edu.academia.applesignon', scope: 'name email', redirectURI: 'https://www.academia.edu/sessions', state: "15798f9f27e022fea8d9a34ff53f6dd4c360f47a950a24227a023669f916b293", });</script><script>// Hacky way of checking if on fast loswp if (window.loswp == null) { (function() { const Google = window?.Aedu?.Auth?.OauthButton?.Login?.Google; const Facebook = window?.Aedu?.Auth?.OauthButton?.Login?.Facebook; if (Google) { new Google({ el: '#login-google-oauth-button', rememberMeCheckboxId: 'remember_me', track: null }); } if (Facebook) { new Facebook({ el: '#login-facebook-oauth-button', rememberMeCheckboxId: 'remember_me', track: null }); } })(); }</script></div></div></div><div class="modal-body"><div class="row"><div class="col-xs-10 col-xs-offset-1"><div class="hr-heading login-hr-heading"><span class="hr-heading-text">or</span></div></div></div></div><div class="modal-body"><div class="row"><div class="col-xs-10 col-xs-offset-1"><form class="js-login-form" action="https://www.academia.edu/sessions" accept-charset="UTF-8" method="post"><input name="utf8" type="hidden" value="✓" autocomplete="off" /><input type="hidden" name="authenticity_token" value="arZnhNwJGFT6wWdIVgRGNS0wOLf0lFfqMnIQjQHvF9G2uRylmoz5aV0LkJU+FbynQ+9Y26vIDXshvpbrI5aZWg==" autocomplete="off" /><div class="form-group"><label class="control-label" for="login-modal-email-input" style="font-size: 14px;">Email</label><input class="form-control" id="login-modal-email-input" name="login" type="email" /></div><div class="form-group"><label class="control-label" for="login-modal-password-input" style="font-size: 14px;">Password</label><input class="form-control" id="login-modal-password-input" name="password" type="password" /></div><input type="hidden" name="post_login_redirect_url" id="post_login_redirect_url" value="https://independent.academia.edu/HarePradhan" autocomplete="off" /><div class="checkbox"><label><input type="checkbox" name="remember_me" id="remember_me" value="1" checked="checked" /><small style="font-size: 12px; margin-top: 2px; display: inline-block;">Remember me on this computer</small></label></div><br><input type="submit" name="commit" value="Log In" class="btn btn-primary btn-block btn-lg js-login-submit" data-disable-with="Log In" /></br></form><script>typeof window?.Aedu?.recaptchaManagedForm === 'function' && window.Aedu.recaptchaManagedForm( document.querySelector('.js-login-form'), document.querySelector('.js-login-submit') );</script><small style="font-size: 12px;"><br />or <a data-target="#login-modal-reset-password-container" data-toggle="collapse" href="javascript:void(0)">reset password</a></small><div class="collapse" id="login-modal-reset-password-container"><br /><div class="well margin-0x"><form class="js-password-reset-form" action="https://www.academia.edu/reset_password" accept-charset="UTF-8" method="post"><input name="utf8" type="hidden" value="✓" autocomplete="off" /><input type="hidden" name="authenticity_token" value="SMiw8LuSfnS7Ig2/hXF3o6G029YIcESkOzoD2Glt/sCUx8vR/RefSRzo+mLtYI0xz2u7ulcsHjUo9oW+SxRwSw==" autocomplete="off" /><p>Enter the email address you signed up with and we'll email you a reset link.</p><div class="form-group"><input class="form-control" name="email" type="email" /></div><script src="https://recaptcha.net/recaptcha/api.js" async defer></script> <script> var invisibleRecaptchaSubmit = function () { var closestForm = function (ele) { var curEle = ele.parentNode; while (curEle.nodeName !== 'FORM' && curEle.nodeName !== 'BODY'){ curEle = curEle.parentNode; } return curEle.nodeName === 'FORM' ? curEle : null }; var eles = document.getElementsByClassName('g-recaptcha'); if (eles.length > 0) { var form = closestForm(eles[0]); if (form) { form.submit(); } } }; </script> <input type="submit" data-sitekey="6Lf3KHUUAAAAACggoMpmGJdQDtiyrjVlvGJ6BbAj" data-callback="invisibleRecaptchaSubmit" class="g-recaptcha btn btn-primary btn-block" value="Email me a link" value=""/> </form></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/collapse-45805421cf446ca5adf7aaa1935b08a3a8d1d9a6cc5d91a62a2a3a00b20b3e6a.js"], function() { // from javascript_helper.rb $("#login-modal-reset-password-container").on("shown.bs.collapse", function() { $(this).find("input[type=email]").focus(); }); }); </script> </div></div></div><div class="modal-footer"><div class="text-center"><small style="font-size: 12px;">Need an account? <a rel="nofollow" href="https://www.academia.edu/signup">Click here to sign up</a></small></div></div></div></div></div></div><script>// If we are on subdomain or non-bootstrapped page, redirect to login page instead of showing modal (function(){ if (typeof $ === 'undefined') return; var host = window.location.hostname; if ((host === $domain || host === "www."+$domain) && (typeof $().modal === 'function')) { $("#nav_log_in").click(function(e) { // Don't follow the link and open the modal e.preventDefault(); $("#login-modal").on('shown.bs.modal', function() { $(this).find("#login-modal-email-input").focus() }).modal('show'); }); } })()</script> <div class="bootstrap" id="footer"><div class="footer-content clearfix text-center padding-top-7x" style="width:100%;"><ul class="footer-links-secondary footer-links-wide list-inline margin-bottom-1x"><li><a href="https://www.academia.edu/about">About</a></li><li><a href="https://www.academia.edu/press">Press</a></li><li><a rel="nofollow" href="https://medium.com/academia">Blog</a></li><li><a href="https://www.academia.edu/documents">Papers</a></li><li><a href="https://www.academia.edu/topics">Topics</a></li><li><a href="https://www.academia.edu/journals">Academia.edu Journals</a></li><li><a rel="nofollow" href="https://www.academia.edu/hiring"><svg style="width: 13px; height: 13px;" aria-hidden="true" focusable="false" data-prefix="fas" data-icon="briefcase" class="svg-inline--fa fa-briefcase fa-w-16" role="img" xmlns="http://www.w3.org/2000/svg" viewBox="0 0 512 512"><path fill="currentColor" d="M320 336c0 8.84-7.16 16-16 16h-96c-8.84 0-16-7.16-16-16v-48H0v144c0 25.6 22.4 48 48 48h416c25.6 0 48-22.4 48-48V288H320v48zm144-208h-80V80c0-25.6-22.4-48-48-48H176c-25.6 0-48 22.4-48 48v48H48c-25.6 0-48 22.4-48 48v80h512v-80c0-25.6-22.4-48-48-48zm-144 0H192V96h128v32z"></path></svg> <strong>We're Hiring!</strong></a></li><li><a rel="nofollow" href="https://support.academia.edu/"><svg style="width: 12px; height: 12px;" aria-hidden="true" focusable="false" data-prefix="fas" data-icon="question-circle" class="svg-inline--fa fa-question-circle fa-w-16" role="img" xmlns="http://www.w3.org/2000/svg" viewBox="0 0 512 512"><path fill="currentColor" d="M504 256c0 136.997-111.043 248-248 248S8 392.997 8 256C8 119.083 119.043 8 256 8s248 111.083 248 248zM262.655 90c-54.497 0-89.255 22.957-116.549 63.758-3.536 5.286-2.353 12.415 2.715 16.258l34.699 26.31c5.205 3.947 12.621 3.008 16.665-2.122 17.864-22.658 30.113-35.797 57.303-35.797 20.429 0 45.698 13.148 45.698 32.958 0 14.976-12.363 22.667-32.534 33.976C247.128 238.528 216 254.941 216 296v4c0 6.627 5.373 12 12 12h56c6.627 0 12-5.373 12-12v-1.333c0-28.462 83.186-29.647 83.186-106.667 0-58.002-60.165-102-116.531-102zM256 338c-25.365 0-46 20.635-46 46 0 25.364 20.635 46 46 46s46-20.636 46-46c0-25.365-20.635-46-46-46z"></path></svg> <strong>Help Center</strong></a></li></ul><ul class="footer-links-tertiary list-inline margin-bottom-1x"><li class="small">Find new research papers in:</li><li class="small"><a href="https://www.academia.edu/Documents/in/Physics">Physics</a></li><li class="small"><a href="https://www.academia.edu/Documents/in/Chemistry">Chemistry</a></li><li class="small"><a href="https://www.academia.edu/Documents/in/Biology">Biology</a></li><li class="small"><a href="https://www.academia.edu/Documents/in/Health_Sciences">Health Sciences</a></li><li class="small"><a href="https://www.academia.edu/Documents/in/Ecology">Ecology</a></li><li class="small"><a href="https://www.academia.edu/Documents/in/Earth_Sciences">Earth Sciences</a></li><li class="small"><a href="https://www.academia.edu/Documents/in/Cognitive_Science">Cognitive Science</a></li><li class="small"><a href="https://www.academia.edu/Documents/in/Mathematics">Mathematics</a></li><li class="small"><a href="https://www.academia.edu/Documents/in/Computer_Science">Computer Science</a></li></ul></div></div><div class="DesignSystem" id="credit" style="width:100%;"><ul class="u-pl0x footer-links-legal list-inline"><li><a rel="nofollow" href="https://www.academia.edu/terms">Terms</a></li><li><a rel="nofollow" href="https://www.academia.edu/privacy">Privacy</a></li><li><a rel="nofollow" href="https://www.academia.edu/copyright">Copyright</a></li><li>Academia ©2024</li></ul></div><script> //<![CDATA[ window.detect_gmtoffset = true; window.Academia && window.Academia.set_gmtoffset && Academia.set_gmtoffset('/gmtoffset'); //]]> </script> <div id='overlay_background'></div> <div id='bootstrap-modal-container' class='bootstrap'></div> <div id='ds-modal-container' class='bootstrap DesignSystem'></div> <div id='full-screen-modal'></div> </div> </body> </html>