Automated HCP Assays & Protein A Assays with Simple Plex

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<div class="highlighted"> <aside class="container section clearfix" role="complementary"> <div> </div> </aside> </div> <div id="main" class="container-fluid container-wide "> <div class="row"> <div class="col-md-12"> <div> <div id="block-bio-techne-global-breadcrumbs" class="system_breadcrumb_block"> <nav role="navigation" aria-labelledby="system-breadcrumb"> <h2 id="system-breadcrumb" class="visually-hidden">Breadcrumb</h2> <ol> <li> <a href="/">Home</a> </li> <li> <a href="/resources">Resources</a> </li> <li> <a href="/resources/instrument-applications">ProteinSimple Instrument Applications</a> </li> <li> Simple Plex For Bioprocess Development </li> </ol> </nav> </div> </div> </div> </div> <div class="row"> <div class="col-md-12"> <main> <section class="section"> <div> <div id="block-bio-techne-global-content" class="system_main_block"> <article> <div> <div> <div> <div class="paragraph paragraph--type--section paragraph--view-mode--default"> <div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><a name="highlights"></a><a href="#highlights">Product Highlights</a> | <a href="#bioprocessing">Bioprocess Assays</a> | <a href="#benefits">Benefits</a> | <a href="#applications">Applications and Resources</a></p></div> </div> </div> <div> <div class="paragraph paragraph--type--_-2-text-to-media mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_header_2_global"> <div><h2>Simple Plex Assays for Bioprocess Testing</h2></div> </div> <div class="field_block:paragraph:2_2_text_to_media:field_lg_sngl_frmtd_text_global"> <div><p><strong><a name="bioprocessing"></a>See how Simple Plex<sup>™</sup> assays on the Ella<sup>™</sup> platform can help automate your process impurity testing workflow and get the data you need quickly and reliably.  </strong><br> <br> Identifying the most promising therapeutic candidates requires a highly sensitive immunoassay solution capable of detecting residual impurities with superior speed, precision and accuracy. Automated Simple Plex assays on Ella offer a consistent, reliable, hands-free solution designed to deliver fast and accurate quantitative results without the hassle of long and complicated workflows.    </p></div> </div> <div class="field_block:paragraph:2_2_text_to_media:field_call_to_action_global"> <a href="/p/instruments/simple-plex/request-quote?productName=Ella%20Automated%20Immunoassay%20System&catalogNumber=600-100" class="btn btn-primary" type="link"> Request Information </a> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_indv_media"> <div> <article> <div class="youtube-video-place"> <lite-youtube videoid="Qe2UyN6SBjU" params="rel=0" style="background-image: url('https://img.youtube.com/vi/Qe2UyN6SBjU/maxresdefault.jpg');"> <a href="https://www.youtube.com/embed/Qe2UyN6SBjU?autoplay=1" class="lty-playbtn" title="Ella Instrument: Precision Multiplexing Made Simple"> <span class="lyt-visually-hidden">Ella Instrument: Precision Multiplexing Made Simple</span> </a> </lite-youtube> </div> </article> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--section paragraph--view-mode--default mb-4"> <div> <div> <div class="paragraph paragraph--type--_-2-text-to-media mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_header_2_global"> <div><h2>Simple Plex Bioprocessing Assays</h2></div> </div> <div class="field_block:paragraph:2_2_text_to_media:field_lg_sngl_frmtd_text_global"> <div><p>To meet increasing throughput and safety demands, fast and reproducible detection of host cell proteins (HCPs) is essential during the manufacturing process of a biotherapeutic. The reduction in manual steps inherent in Ella’s automated workflow enables a high level of reproducibility and data quality — all with a method that’s fast and easy to transfer.</p></div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/bulk-protein-ribbon600x325.png" alt="Bulk Protein Ribbon" loading="lazy" > </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--_-2-text-to-media mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_header_2_global"> <div><h3>Simple Plex Protein A Assay</h3></div> </div> <div class="field_block:paragraph:2_2_text_to_media:field_lg_sngl_frmtd_text_global"> <div><p>Quickly and accurately detect and quantify Protein A impurities with the Simple Plex Protein A assay on Ella. The assay offers an automated solution to get the data you need quickly while ensuring consistent and reliable results for your bioprocess workflow.</p> <ul> <li>Wide dynamic range reduces sample dilutions while maintaining sensitivity.</li> <li>Less hands-on time with built-in standard curves.</li> <li>Easier sample prep with simplified pretreatment.</li> </ul></div> </div> <div class="field_block:paragraph:2_2_text_to_media:field_call_to_action_global"> <a href="/p/simple-plex/simple-plex-protein-a-cartridge_spckb-ot-009204" class="btn btn-primary" type="link"> Purchase the Protein A Assay </a> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/applications/ella-700x370-immune-cell-therapy-3.png" alt="Ella instrument with assay cartridge" loading="lazy" > </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--section paragraph--view-mode--default mb-4"> <div> <div> <div class="paragraph paragraph--type--_-2-media-to-text mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_media_to_text:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/instruments/500x280-simple-plex-lab-life-1.png" alt="Scientist prepares a sample to run automated ELISA on Ella." loading="lazy" > </div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_media_to_text:field_header_2_global"> <div><h3>Rapid Endonuclease Quantification</h3></div> </div> <div class="field_block:paragraph:2_2_media_to_text:field_lg_sngl_frmtd_text_global"> <div><p>The Simple Plex Endonuclease assay is a selective and sensitive method for detecting residual endonuclease impurities in bioprocess samples. Specificity testing has demonstrated that this assay detects both Benzonase<sup>®</sup> and Denarase<sup>®</sup>.</p></div> </div> <div class="field_block:paragraph:2_2_media_to_text:field_call_to_action_global"> <a href="/resources/literature/rapid-endonuclease-quantification" class="btn btn-primary" type="link"> Download The Application Note </a> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--_-2-media-to-text mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_media_to_text:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/lg-hek-cell-teaser-525x410.png" alt="3D realistic illustration of HEK cells" loading="lazy" > </div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_media_to_text:field_header_2_global"> <div><h3>Simple Plex HCP Assays with Cygnus Technologies</h3></div> </div> <div class="field_block:paragraph:2_2_media_to_text:field_lg_sngl_frmtd_text_global"> <div><p>To bring you the best quality immunoassays, we've partnered with the industry leader in bioprocess impurity testing. <a href="https://www.cygnustechnologies.com/" target="_blank">Cygnus Technologies</a> provides the highest level of validation and characterization of the antibodies that go into their assays, ensuring that HCP coverage is complete and reproducible.</p> <p>The Simple Plex CHO HCP 3G-1 assay (SPCKB-OT-003714) and HEK 293 HCP 3G (SPCKB-OT-007066) on Ella are based on the same antibodies currently used in the Cygnus CHO HCP ELISA Kit, 3G (F550-1) and HEK 293 HCP ELISA kit, 3G Resupply 1 (F650S), respectively. These antibodies have been evaluated for reactivity to more than 1,000 individual HCPs present in CHO or HEK 293 strains by state-of-the-art <a href="https://www.cygnustechnologies.com/media/productattach/c/y/cygnus-aae-whitepaper_f_v3.pdf" target="_blank">Antibody Affinity Extraction<sup>™</sup></a> and mass spectrometry methods.</p></div> </div> <div class="field_block:paragraph:2_2_media_to_text:field_call_to_action_global"> <a href="https://www.cygnustechnologies.com/custom-development-services/assay-qualification" class="btn btn-primary" type="link"> Learn More About Cygnus </a> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--button-row paragraph--view-mode--default"> <div class="row"> <div class="col"> <div class="list-grid-wrap"> <div class="row"> <div class="col-md-4"> <a href="/p/simple-plex/simple-plex-cho-hcp-3g-1-cartridge_spckb-ot-003714" class="btn btn-primary" type="link"> View CHO HCP 3G-1 Assay </a> </div> <div class="col-md-4"> <a href="/p/simple-plex/simple-plex-hek-293-hcp-3g-cartridge_spckb-ot-007066" class="btn btn-primary" type="link"> View HEK 293 HCP 3G Assay </a> </div> <div class="col-md-4"> <a href="/p/simple-plex/simple-plex-endonuclease-cartridge_spckc-ot-008612" class="btn btn-primary" type="link"> View Endonuclease Assay </a> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><a name="benefits"></a></p></div> </div> </div> <div> <div class="paragraph paragraph--type--card-paragraph paragraph--view-mode--default card-para-default"> <h2>Accelerate Your Process Impurity Testing with Ella</h2> <div class="row card_row"> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/reagents/simple-plex-immunoassays/assay-menu" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: #00263A; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/lg-bioprocess-icon-assays-dark-300x300-v2.png" alt="Assay icon " loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="/reagents/simple-plex-immunoassays/assay-menu" > <h3 class="card_title_htag"> <div><h3>Precise Quantification</h3></div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>Obtain superior assay specificity and sensitivity at each stage of the purification process. Sub-picogram level detection and 4+ logs of dynamic range provide confirmation that residual contaminants are within required limits.</p></div> </div> <div class="card-bottom-link"> <div> <a href="/reagents/simple-plex-immunoassays/assay-menu" > View Assay Menu </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/instruments/simple-plex" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: #00263A; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/lg-bioprocess-icon-target-dark-300x300-v2.png" alt="target icon" loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="/instruments/simple-plex" > <h3 class="card_title_htag"> <div><h3>Consistent Results</h3></div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>Get consistent, accurate assay results as part of an efficient, hands-free workflow. Advanced microfluidic circuitry performs every step of the assay process, reducing variability and minimizing user error.</p></div> </div> <div class="card-bottom-link"> <div> <a href="/instruments/simple-plex" > Learn More </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/instruments/simple-plex/cost-advantages" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: #00263A; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/lg-bioprocess-icon-stopwatch-timer-dark-300x300-v2.png" alt="stopwatch icon" loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="/instruments/simple-plex/cost-advantages" > <h3 class="card_title_htag"> <div><h3>Higher Throughput</h3></div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>Boost your process development throughput with fully validated results in 90 minutes or less. Detect and quantify process impurities with superior speed, accuracy and consistency not possible with a traditional ELISA.</p></div> </div> <div class="card-bottom-link"> <div> <a href="/instruments/simple-plex/cost-advantages" > Compare to ELISA </a> </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--_-3-media-to-text paragraph--view-mode--default mb-4 p-3"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--25-75"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:1_3_media_to_text:field_indv_media"> <div> <a href="https://www.future-science.com/doi/10.2144/btn-2020-0074" title="Read the Biotechniques Publication" target="_blank" rel="noreferrer" > <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/lg-bioprocess-icon-notes-light-300x300.png" alt="note icon" loading="lazy" > </a> </div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:1_3_media_to_text:field_header_2_global"> <div><h2>Ella helps GSK Establish Walk-Away Method for CHO HCP Impurity Analysis</h2></div> </div> <div class="field_block:paragraph:1_3_media_to_text:field_lg_sngl_frmtd_text_global"> <div><p>Biopharmaceutical analytical scientists at <a href="https://www.gsk.com/en-gb" target="_blank">GSK</a> use Ella to accurately quantitate HCP concentrations throughout process development. Read this <em>Biotechniques</em> publication to learn how Ella is allowing their scientists to quickly report CHO HCP trends and speed up process-related decision-making and batch release of drug product.<a name="applications"></a></p></div> </div> <div class="field_block:paragraph:1_3_media_to_text:field_call_to_action_global"> <a href="https://www.future-science.com/doi/10.2144/btn-2020-0074" class="btn btn-primary" type="link"> Read the Biotechniques Publication </a> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--section paragraph--view-mode--default"> <div> <div> <div class="paragraph paragraph--type--button-row paragraph--view-mode--default"> <div class="row"> <div class="col"> <div><h2>Ella Applications</h2></div> <div class="list-grid-wrap"> <div class="row"> <div class="col-md-4"> <a href="/resources/instrument-applications/simple-plex-covid-19-research" class="btn btn-primary" type="link"> Assays for COVID-19 Research </a> </div> <div class="col-md-4"> <a href="/resources/instrument-applications/simple-plex-inflammation" class="btn btn-primary" type="link"> Profile Inflammatory Responses </a> </div> <div class="col-md-4"> <a href="/resources/instrument-applications/simple-plex-cancer-biomarkers" class="btn btn-primary" type="link"> Quantify Key Cancer Biomarkers </a> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--button-row paragraph--view-mode--default"> <div class="row"> <div class="col"> <div class="list-grid-wrap"> <div class="row"> <div class="col-md-4"> <a href="/resources/instrument-applications/simple-plex-cell-therapy" class="btn btn-primary" type="link"> Cell and Gene Therapy Assays </a> </div> <div class="col-md-4"> <a href="/resources/instrument-applications/simple-plex-bioprocess-development" class="btn btn-primary" type="link"> Expedite Bioprocessing </a> </div> <div class="col-md-4"> <a href="/resources/instrument-applications/simple-plex-preclinical-research" class="btn btn-primary" type="link"> Preclinical Assays </a> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph accordion mb-4 paragraph--type--extendable-paragraph paragraph--view-mode--default"> <div class="card-header rounded-top bg-white"> <a class="collapsed" data-toggle="collapse" href="#expPara-additional-ella-resources7" role="button" aria-expanded="false" aria-controls="expPara"> <h5>Additional Ella Resources</h5> </a> </div> <div class="collapse" id="expPara-additional-ella-resources7" aria-expanded="false" aria-controls="expPara"> <div class="card-body p-4"> <br/> <div><ul> <li><a href="https://resources.bio-techne.com/bio-techne-assets/docs/literature/simple-plex-ella-instrument.pdf" target="_blank">Download Ella Brochure</a></li> <li><a href="/resources/testimonials?tid%5B377%5D=377" target="_self">Read Customer Stories</a></li> <li><a href="/resources/literature/rapid-endonuclease-quantification" target="_self">Download the Endonuclease Application Note </a></li> <li><a href="/resources/webinars/streamline-cell-and-gene-therapy-with-simple-western" target="_self">Webinar: Streamline Cell and Gene Therapy Workflow</a></li> <li><a href="https://resources.bio-techne.com/bio-techne-assets/docs/literature/cell-kill-appnote.pdf" target="_blank">Download the T Cell Activation Marker App Note </a></li> </ul></div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p>Benzonase<sup>®</sup> is a registered trademark of Merck KGaA Corporation. 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