BCR Repertoire Analysis Services

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We are committed to providing highly customized comprehensive solutions with the best quality to advance your projects. </p> <b class="Highlights_tit">Highlights</b> <ul class="HighlightsArea"> <li class="col-md-6 col-lg-6 col-sm-6"> <img src="static/images/Abouts-menu-lights-pic-1.jpg" srcset="static/images/Abouts-menu-lights-pic-1.webp 1x" width="200" height="131" loading="lazy"><a href="aboutus.html" class="bannerbutton"> <i class="fa fa-angle-right"></i>About CBL </a> </li> <li class="col-md-6 col-lg-6 col-sm-6"> <img src="static/images/Abouts-menu-lights-pic-2.jpg" srcset="static/images/Abouts-menu-lights-pic-2.webp 1x" width="200" height="131" loading="lazy"><a href="contactus.html" class="bannerbutton"> <i class="fa fa-angle-right"></i>Contact Us </a> </li> </ul> </li> </ul> </div> </div> </li> </ul> <script> window.addEventListener('load',function(){ var arr = ['/drug-discovery/therapeutics/','/bioconjugation/','/adc/']; $('.mennav ul li a').each(function(){ var $this = $(this); var href = $this.attr("href").toLocaleLowerCase(); $.each(arr, function(index, value) { if (href && href.startsWith(value)) { $this.attr('target', '_blank'); } }); }); }); </script> </div> <div class="top-search search-group"> <div class="col-md-5 col-sm-6"> <label class="radio-inline"><input type="radio" name="search" class="rdo_productsearch" value="product" data-type="product" placeholder="Please input cat#, product name, abbr, or keywords."> Product</label> <label class="radio-inline"><input type="radio" name="search" class="rdo_servicesearch" value="service" data-type="service" placeholder="Please input your keywords."> Service</label> <label class="radio-inline"><input type="radio" name="search" class="rdo_googlesearch" value="google" data-type="google" placeholder="Please input your keywords." checked="checked"> Keyword</label> </div> <div class="col-md-7 col-sm-6 search-div"> <input id="keys" name="keys" placeholder="Please input your keywords." class="search-input"> <div class="selectItems"></div> <span class="search_btn"><i class="fa fa-search"></i></span> </div> </div> </div> <div class="searchShow"> <div class="searchtopbg"></div> <span class="searchclosed"><i class="fa fa-times"></i>Close</span> <div class="addsearch search-group"></div> </div> <div class="banner_inpage services_bg_2"> <div class="auto-container small_content-width about_container"> <ul class="bread-crumb clearfix"> <li><a href="/">Home</a></li> <li><a href="javascrpt:void(0)">Characterization</a></li><li><a href="/immunologic-analysis-services.html">Immunogenicity Analysis</a></li><li><a href="/Magic-Therapeutic-Antibody-Discovery-Service.htm">Repertoire Analysis</a></li> <li>BCR Repertoire Analysis</li> </ul> <p>"Creative Biolabs is committed to providing highly customized comprehensive solutions with the best quality to advance our global clients’ projects."</p> </div> </div> <script> window.addEventListener('load', function () { if (($('.bread-crumb li:last-child').text()) == "Peer-Reviewed Publications") { $(".banner_inpage").addClass("peer-reviewed-publications_bg").removeClass("services_bg_2"); } if ($(window).hasClass('bread-crumb')) { if ($(".bread-crumb").html().indexOf("RESOURCE") > -1) { $(".pronote").hide(); } if ($(".bread-crumb").html().indexOf("Careers") > -1) { $(".pronote").hide(); } } }); </script> <div class="small_content-width services-container"> <div class="col-md-9"> <div class="inpage_section_title"> <h1>BCR Repertoire Analysis Services</h1> </div> <div class="page-tab"> <span onclick="window.location.href=window.location.pathname+'#tab1'">Background</span> <span onclick="window.location.href=window.location.pathname+'#tab2'">Service</span> <span onclick="window.location.href=window.location.pathname+'#tab3'">Features</span> <span onclick="window.location.href=window.location.pathname+'#tab4'">BCR repertoire analysis</span> <span onclick="window.location.href=window.location.pathname+'#tab5'">FAQ</span> <span onclick="window.location.href=window.location.pathname+'#tab6'">Resources</span> </div> <script>window.addEventListener('load',function(){page_tab_margin();page_tab_css();})</script> <p> <strong>Creative Biolabs</strong> offers the unparalleled mass sequencing service to analyze the B-cell receptor (BCR) repertoires of human or mice. According to the Magic™ platform which mainly based on next generation sequencing (NGS) technique, our scientists are able to identify the vast majority of BCR V(D)JC sequences contained in a single sample. </p> <h2 class="h2_tit page_tab_margin" id="tab1"> Background </h2> <p> BCR is an indispensable component of a potent adaptive immune system, which is fundamentally reliant upon its diverse repertoire. BCR is generally defined as a transmembrane receptor located on the B-cell surface or a kind of membrane-bound antibody form. BCRs are assembled during the B-cell development and went through somatic recombination of three gene segments of the heavy chain locus (V, D, J) and two gene segments of the light chain locus (V, J) to diversify variable region gene. According to the rearrangement mechanism, there are no the same BCRs between two different B-cells, which enable the repertoire of BCRs to become a valuable tool for the investigation of the immune system. In addition, through exposure to endogenous host factors and exogenous invaders, the BCR repertoire is continuously shaped and changed which establishes a series of potential associations with infections or diseases. In this way, high-throughput sequencing is a very useful method to investigate and analyze the change of BCR repertoire in some specific physiological processes, which may include autoimmune and tumor diseases, and then be helpful to discover new therapeutic agents, such as specific antibodies for interested targets and effective vaccines. </p> <p class="ServiceShowPic"> <img src="static/img/BCR-Repertoire-Analysis.jpg" width="500" height="349" loading="lazy" alt="Fig.1 Repertoire sequencing data production." /> Fig.1 BCR Repertoire Analysis.<sup>1, 3</sup> </p> <h2 class="h2_tit page_tab_margin" id="tab2"> Magic™ BCR Repertoire Sequencing Service </h2> <p> <strong>Creative Biolabs</strong> has established the high-throughput Magic™ platform for large-scale sequencing services. To achieve immune repertoire sequencing (IR-seq), RNA from whole blood or B cells is the required template for the amplification of complementary determining region (CDR), and then the Magic™ platform can be used to provide one-stop service including high-throughput sequencing and data analysis. Through our Magic™ IR-seq platform, both the heavy chain and the light chain CDR 3 regions of either human or mice can be 100% covered and accurately sequenced, the constant regions from the two species are also captured and sequenced with enough length to identify the class information. In this way, our customers can finally obtain a comprehensive BCR repertoire involving both the hypermutation and isotype data from this sequencing service. </p> <h2 class="h2_tit page_tab_margin" id="tab3"> Features of Magic™ platform for repertoire analysis </h2> <ul class="ullist"> <li> Comprehensive BCR repertoire from RNA of either human or mice </li> <li> Specific capture primers </li> <li> High fidelity amplification method </li> <li> Advanced high-throughput sequencing platform </li> </ul> <br /> <p> With years of research and development experience in the field of immunology, <strong>Creative Biolabs</strong> has owned a group of scientists who have the distinguished understanding of the adaptive immune system. Through the one-stop BCR repertoires analysis service, our seasoned scientists are pleased to share expertise and experience with our global clients and facilitate their meaningful immune repertoire researches. </p> <p> Learn more about Magic™ BCR repertoire analysis services: </p> <ul class="ullist"> <li> <a href="/magic-unbiased-bcr-repertoire-profiling.html">Magic™ Unbiased BCR Repertoire Profiling</a> </li> <li> <a href="/magic-single-cell-bcr-profiling.html">Magic™ Single-cell BCR Profiling</a> </li> <li> <a href="/magic-single-cell-bcr-profiling.html">Magic™ Comprehensive MicroRNA Profiling of B Cells</a> </li> </ul> <h2 class="h2_tit page_tab_margin" id="tab4"> BCR repertoire analysis </h2> <ul class="ullist"> <li> <strong>Identification of Specific V and J Genes and the Assessment of Clonality and Diversity by BCR Repertoire Analysis</strong> </li> </ul> <p class="show-center"> <img loading="lazy" src="static/img/1-1-1-2-3-4-magic-bcr-repertoire-analysis-2.jpg" width="300" height="448" alt="Fig.2 B-cell receptor repertoire construction and analysis." />Fig.2 BCR repertoire construction and analysis.<sup>2, 3</sup> </p> <p> The study reveals immune heterogeneity between systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA) using multi-omics data, particularly focusing on T-cell receptor (TCR) and B-cell receptor (BCR) repertoires. BCR repertoire analysis, a critical component of this research, allows for the identification of specific V and J genes and the assessment of clonality and diversity, providing insights into the distinct immune responses in SLE and RA. The results show significant differences in CDR3 amino acid sequence diversity, TRBV, TRBJ, IGHV, and IGHJ gene usage between SLE, RA, and healthy controls. These findings contribute to understanding the pathogenesis of these autoimmune diseases, suggesting potential diagnostic markers and therapeutic targets. The application of BCR repertoire analysis in this study underscores its importance in deciphering the complex immune landscapes of autoimmune diseases, potentially guiding the development of personalized treatments. </p> <div class="reference"> <strong>References</strong> <ol class="ollist decimal"> <li> Yaari, Gur, and Steven H. Kleinstein. "Practical guidelines for B-cell receptor repertoire sequencing analysis." Genome medicine 7 (2015): 1-14. </li> <li> Zhang, Yuntian, and Tzong-Yi Lee. "Revealing the immune heterogeneity between systemic lupus erythematosus and rheumatoid arthritis based on multi-omics data analysis." International journal of molecular sciences 23.9 (2022): 5166. </li> <li> Distributed under Open Access license <a href="https://creativecommons.org/licenses/by/4.0/deed">CC BY 4.0</a>. The image was modified by revising the title. </li> </ol> </div> <script type="text/javascript"> window.addEventListener('load',function(){ faqlist(4); }); </script> <h2 class="h2_tit page_tab_margin" id="tab5"> FAQ </h2> <ol class="ollist faqlist"> <li> <span class="sFAQTit">What is BCR repertoire analysis and why is it important in immunology research?</span> <p> BCR repertoire analysis involves the sequencing and study of B-cell receptor (BCR) diversity within an organism's immune system. It provides detailed insights into the variety of antibodies produced by B cells, which is crucial for understanding immune responses, disease mechanisms, and the development of targeted therapies. This analysis is particularly important in autoimmune diseases, cancer, and infectious disease research. </p> </li> <li> <span class="sFAQTit">How is BCR repertoire analysis performed?</span> <p> BCR repertoire analysis is typically performed using high-throughput sequencing techniques, such as next-generation sequencing (NGS). This process involves isolating B cells, extracting RNA or DNA, amplifying the BCR regions (usually the V, D, and J segments), and then sequencing these regions to identify the different BCR sequences. Bioinformatics tools are then used to analyze the diversity and clonality of the BCR repertoire. </p> </li> <li> <span class="sFAQTit">What are the key applications of BCR repertoire analysis in clinical research?</span> <p> BCR repertoire analysis has several critical applications in tracking disease progression, monitoring immune responses to vaccines, identifying biomarkers for diseases such as cancer and autoimmune disorders, and evaluating the effectiveness of immunotherapies. It is also used in understanding the mechanisms of chronic infections and developing new therapeutic strategies. </p> </li> <li> <span class="sFAQTit">How does BCR repertoire analysis contribute to the development of personalized medicine?</span> <p> BCR repertoire analysis enables the detailed characterization of an individual's immune response by identifying unique BCR sequences. This information can be used to tailor treatments to the individual's specific immune profile, leading to more effective and personalized therapeutic interventions. It is particularly valuable in cancer immunotherapy, where patient-specific BCR profiles guide the selection of targeted treatments. </p> </li> <li> <span class="sFAQTit">What are the challenges associated with BCR repertoire analysis?</span> <p> One of the main challenges of BCR Repertoire Analysis is the sheer complexity and diversity of the BCR repertoire, which requires advanced bioinformatics tools to accurately analyze and interpret. Additionally, the need for high-quality samples and the technical difficulties in isolating and sequencing BCRs can pose significant challenges. Data interpretation also requires a deep understanding of immunology and computational biology. </p> </li> <li> <span class="sFAQTit">How does BCR repertoire analysis differ from TCR Repertoire Analysis?</span> <p> While both BCR and TCR repertoire analysis focus on understanding the diversity of immune receptors, BCR repertoire analysis studies the antibodies produced by B cells, whereas TCR repertoire analysis focuses on the receptors on T cells. BCRs are involved in recognizing antigens directly, leading to antibody production, while TCRs recognize antigens presented by other cells, leading to a broader immune response. </p> </li> <li> <span class="sFAQTit">What role does BCR repertoire analysis play in vaccine development?</span> <p> BCR repertoire analysis helps researchers understand how the immune system responds to different antigens introduced by vaccines. By analyzing the diversity and specificity of BCRs generated in response to a vaccine, researchers can assess the vaccine's effectiveness and optimize its formulation to induce a strong, long-lasting immune response. </p> </li> <li> <span class="sFAQTit">How does BCR repertoire analysis help in monitoring cancer immunotherapy?</span> <p> In cancer immunotherapy, BCR repertoire analysis is used to monitor the changes in a patient's immune response over time. By tracking the clonality and diversity of BCRs, researchers can evaluate how well the immune system is targeting cancer cells, identify potential resistance mechanisms, and adjust treatment strategies accordingly. This analysis is particularly useful in therapies like CAR-T cell therapy. </p> </li> </ol> <h2 class="h2_tit page_tab_margin" id="tab6"> Resources </h2> <p> Use the resources in our library to help you understand your options and make critical decisions for your study. </p> <div class="tab_nav"> <span class="tab_choosebg">Videos</span> <span>Podcasts</span> <span>Infographic</span> <span>Flyer</span> <span>Articles</span> </div>  <div class="tab_cont" style="display:block;"> <div class="row pagerow_pt"> <div class="col-md-4"> <div class="pagerow_pt_unit"> <img loading="lazy" src="../static/img/video-phage-display-technology.png" alt="Phage Display Technology" /> </div> <a href="https://youtu.be/_Cqj5gphNug" target="_blank">Antibody based Cancer Therapies </a> </div> </div> </div>  <div class="tab_cont"> <div class="therapy_ad"> <a href="therapeutic-antibody.html"> <img loading="lazy" src="../static/img/podcasts-ad.png" width="1600" height="300" srcset="../static/img/podcasts-ad.webp 1x,../static/img/podcasts-ad.webp 2x,../static/img/podcasts-ad.webp 3x" alt="In this brand new podcast series, we will show you everything you want to know about CAR-T therapy, including the mechanism, current applications, technology limitations, and potential strategies." /></a> </div> </div>  <div class="tab_cont"> <div class="row pagerow_pt"> <div class="col-md-4"> <div class="pagerow_pt_unit_2"> <img loading="lazy" src="static/img/antibody-based-therapies-creative-biolabs-11.jpg" width="600" height="500" alt="Antibody-Based Therapies" /> </div> <a href="https://go.creative-biolabs.com/antibody-based-therapies.html">Antibody-Based Therapies</a> </div> <div class="col-md-4"> <div class="pagerow_pt_unit_2"> <img loading="lazy" src="static/img/Development-of-Monoclonal-Antibodies-1.jpg" width="600" height="500" alt="Development of Monoclonal Antibodies" /> </div> <a href="https://go.creative-biolabs.com/development-of-monoclonal-antibodies.html">Development of Monoclonal Antibodies</a> </div> </div> </div>  <div class="tab_cont"> <div class="row pagerow_pt"> <div class="col-md-4"> <div class="pagerow_pt_unit_2"> <img loading="lazy" src="/resource/images/Phage-Display-Technology-Flyer-Creative-Biolabs.jpg" width="600" height="500" alt="Phage Display Technology in Creative Biolabs" /> </div> <a href="/resource/pdf/com/flyers/Phage-Display-Technology-Flyer-Creative-Biolabs.pdf">Phage Display Technology in Creative Biolabs</a> </div> <div class="col-md-4"> <div class="pagerow_pt_unit_2"> <img loading="lazy" src="/resource/images/Immune-Phage-Display-Library-Construction-and-Screening-Flyer-Creative-Biolabs.jpg" width="600" height="500" alt="Antibody Development by Immune Phage Library" /> </div> <a href="/resource/pdf/com/flyers/Immune-Phage-Display-Library-Construction-and-Screening-Flyer-Creative-Biolabs.pdf">Antibody Development by Immune Phage Library</a> </div> <div class="col-md-4"> <div class="pagerow_pt_unit_2"> <img loading="lazy" src="/resource/images/Magic-Therapeutic-Antibody-Discovery-Platform-Flyer-Creative-Biolabs.jpg" width="600" height="500" alt="MagicTM Therapeutic Antibody Discovery Platform" /> </div> <a href="/resource/pdf/com/flyers/Magic-Therapeutic-Antibody-Discovery-Platform-Flyer-Creative-Biolabs.pdf">Magic™ Therapeutic Antibody Discovery Platform</a> </div> </div> </div>  <div class="tab_cont"> <div class="row pagerow_pt"> <div class="col-md-4"> <div class="pagerow_pt_unit"> <img loading="lazy" src="../static/img/Phage-Display-Technology-as-a-Powerful-Platform-for-Antibody-Drug-Discovery-2.jpg" width="600" height="500" alt="Phage Display Technology as a Powerful Platform for Antibody Drug Discovery" /> </div> <a href="phage-display-technology-as-a-powerful-platform-for-antibody-drug-discovery.html">Phage Display Technology as a Powerful Platform for Antibody Drug Discovery</a> </div> <div class="col-md-4"> <div class="pagerow_pt_unit"> <img loading="lazy" src="/static/img/Therapeutic-Monoclona-Antibody-Therapies-in-Chronic-Autoimmune-Demyelinating-Neuropathies-1.jpg" width="600" height="500" alt="Therapeutic Monoclonal Antibody Therapies in Chronic Autoimmune Demyelinating Neuropathies" /> </div> <a href="therapeutic-monoclonal-antibody-therapies-in-chronic-autoimmune-demyelinating-neuropathies.html">Therapeutic Monoclonal Antibody Therapies in Chronic Autoimmune Demyelinating Neuropathies</a> </div> <div class="col-md-4"> <div class="pagerow_pt_unit"> <img loading="lazy" src="static/img/4-8-1-overview-of-phage-display-technology-1.jpg" width="600" height="500" alt="Overview of Phage Display Technology" /> </div> <a href="overview-of-phage-display-technology.html">Overview of Phage Display Technology</a> </div> </div> </div> <script> window.addEventListener('load',function(){ // $(function () { $(".tab_nav span").click(function(){ var eqindex=$(this).index(); 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