Hybridoma based Antibody Discovery Services

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We are committed to providing highly customized comprehensive solutions with the best quality to advance your projects. </p> <b class="Highlights_tit">Highlights</b> <ul class="HighlightsArea"> <li class="col-md-6 col-lg-6 col-sm-6"> <img src="static/images/Abouts-menu-lights-pic-1.jpg" srcset="static/images/Abouts-menu-lights-pic-1.webp 1x" width="200" height="131" loading="lazy"><a href="aboutus.html" class="bannerbutton"> <i class="fa fa-angle-right"></i>About CBL </a> </li> <li class="col-md-6 col-lg-6 col-sm-6"> <img src="static/images/Abouts-menu-lights-pic-2.jpg" srcset="static/images/Abouts-menu-lights-pic-2.webp 1x" width="200" height="131" loading="lazy"><a href="contactus.html" class="bannerbutton"> <i class="fa fa-angle-right"></i>Contact Us </a> </li> </ul> </li> </ul> </div> </div> </li> </ul> <script> window.addEventListener('load',function(){ var arr = ['/drug-discovery/therapeutics/','/bioconjugation/','/adc/']; $('.mennav ul li a').each(function(){ var $this = $(this); var href = $this.attr("href").toLocaleLowerCase(); $.each(arr, function(index, value) { if (href && href.startsWith(value)) { $this.attr('target', '_blank'); } }); }); }); </script> </div> <div class="top-search search-group"> <div class="col-md-5 col-sm-6"> <label class="radio-inline"><input type="radio" name="search" class="rdo_productsearch" value="product" data-type="product" placeholder="Please input cat#, product name, abbr, or keywords."> Product</label> <label class="radio-inline"><input type="radio" name="search" class="rdo_servicesearch" value="service" data-type="service" placeholder="Please input your keywords."> Service</label> <label class="radio-inline"><input type="radio" name="search" class="rdo_googlesearch" value="google" data-type="google" placeholder="Please input your keywords." checked="checked"> Keyword</label> </div> <div class="col-md-7 col-sm-6 search-div"> <input id="keys" name="keys" placeholder="Please input your keywords." class="search-input"> <div class="selectItems"></div> <span class="search_btn"><i class="fa fa-search"></i></span> </div> </div> </div> <div class="searchShow"> <div class="searchtopbg"></div> <span class="searchclosed"><i class="fa fa-times"></i>Close</span> <div class="addsearch search-group"></div> </div> <div class="banner_inpage services_bg_2"> <div class="auto-container small_content-width about_container"> <ul class="bread-crumb clearfix"> <li><a href="/">Home</a></li> <li><a href="javascrpt:void(0)">Discovery</a></li><li><a href="javascrpt:void(0)">By Technology</a></li> <li>Hybridoma based Antibody Discovery</li> </ul> <p>"Creative Biolabs is committed to providing highly customized comprehensive solutions with the best quality to advance our global clients’ projects."</p> </div> </div> <script> window.addEventListener('load', function () { if (($('.bread-crumb li:last-child').text()) == "Peer-Reviewed Publications") { $(".banner_inpage").addClass("peer-reviewed-publications_bg").removeClass("services_bg_2"); } if ($(window).hasClass('bread-crumb')) { if ($(".bread-crumb").html().indexOf("RESOURCE") > -1) { $(".pronote").hide(); } if ($(".bread-crumb").html().indexOf("Careers") > -1) { $(".pronote").hide(); } } }); </script> <div class="small_content-width services-container"> <div class="col-md-9"> <div class="inpage_section_title"> <h1>Hybridoma based Antibody Discovery Services</h1> </div> <div class="page-tab"> <span onclick="window.location.href=window.location.pathname+'#tab1'">Introduction</span> <span onclick="window.location.href=window.location.pathname+'#tab2'">Workflow</span> <span onclick="window.location.href=window.location.pathname+'#tab3'">Custom Services</span> <span onclick="window.location.href=window.location.pathname+'#tab4'">Features</span> <span onclick="window.location.href=window.location.pathname+'#tab5'">Applications</span> <span onclick="window.location.href=window.location.pathname+'#tab6'">Case Study</span> <span onclick="window.location.href=window.location.pathname+'#tab7'">Resources</span> </div> <script>window.addEventListener('load',function(){page_tab_css();page_tab_margin();});</script> <p> <strong>Creative Biolabs</strong> stands as a prominent authority proficient in harnessing hybridoma technology for the advancement of monoclonal antibody (mAb) development. Leveraging our wealth of expertise and cutting-edge framework, we take pride in extending hybridoma solutions across various species to cater to a global clientele. </p> <h2 class="h2_tit page_tab_margin" id="tab1"> Introduction of Hybridoma Services </h2> <p> Hybridoma technology, a time-honored approach for the sustained production of mAb on a large scale, involves the fusion of specific antibody-producing B lymphoblasts with myeloma cells, culminating in the generation of hybrid cell lines pivotal to this technique. The process typically commences with animal immunization, followed by the isolation of splenocytes, from which B cells generating target-specific antibodies are extracted and subsequently fused with myeloma cells. These resultant stable hybridoma cells exhibit the capacity for continuous expression of antigen-specific mAbs. These mAbs hold significant value not only in fundamental research within the realms of biology and immunology but also in diverse medical contexts. Furthermore, bolstered by supplementary advanced methodologies offered at Creative Biolabs, such as <a href="/antibody-sequencing-service.html">hybridoma sequencing</a>, <a href="/Production-of-Anti-idiotype-Antibody.html">anti-idiotype Antibody production,</a> and <a href="/adc/one-stop-adc-development-service.htm">antibody-drug conjugates (ADCs)</a>, the expanded utilization of mAbs can significantly contribute to the development of therapeutic or diagnostic modalities. </p> <p class="show-center"> <img loading="lazy" src="static/img/1-1-5-hybridoma-based-antibody-discovery-1.jpg" width="400" height="516" alt="Fig.1 Hybridoma technology. (Creative Biolabs Original)" />Fig.1 Hybridoma technology. </p> <h2 class="h2_tit page_tab_margin" id="tab2"> One-Stop MAb Production <em>via</em> Hybridoma Technology </h2> <div class="row textboxstyle-45 blue45"> <div class="col-md-6"> <div class="textboxstylecont doarea_box"> <span>Antigen Preparation</span> <div class="cont_area"> <ul class="ullist"> <li> Peptides, proteins, enzymes, compounds, DNA, antibodies and eukaryotic cells </li> <li> Immunogen development: Peptides analysis & synthesis, customized recombinant protein expression, Haptens-KLH/OVA conjugation, eukaryotic cell culture </li> </ul> <p> Our team of researchers possesses vast expertise in managing diverse antigen variants. We are proficient in utilizing either purified antigens or commencing with <em>in silico</em> sequences provided by our clients. Leveraging our state-of-the-art recombinant/synthetic systems, we can generate soluble proteins and peptides. Additionally, through our innovative <a href="/Membrane-Protein-Production.html">Magic™ membrane protein production services</a>, <strong>Creative Biolabs</strong> offers the capability to produce membrane proteins in a variety of formats tailored to the specific needs of our clientele. We also provide comprehensive <a href="/immunogen-design-service.html">Services for Immunogen Design</a>, ensuring a tailored approach to meet your specific needs. </p> </div> </div> </div> <div class="col-md-6"> <div class="textboxstylecont doarea_box"> <span>Immunization</span> <div class="cont_area"> <ul class="ullist"> <li> Mouse C57BL/6 and Balb/C </li> <li> Rat Sprague-Dawley and Wistar </li> <li> Immunodeficient mouse </li> <li> Humanization mouse </li> </ul> <p> In response to the unique project requisites of our clientele, we offer <a href="/comprehensive-immunization-strategies-for-novel-antibody-discovery.html">customized immunization strategies</a>, encompassing a range of approaches such as <a href="/antibody-production-through-DNA-immunization-genetic-immunization.html">DNA immunization</a>, <a href="/antibody-production-by-whole-cell-immunization.html">whole cell immunization</a>, and various <a href="/antibody-production-by-conventional-immunization.html">conventional immunization techniques</a>. </p> </div> </div> </div> </div> <div class="row textboxstyle-45 blue45"> <div class="col-md-12"> <div class="textboxstylecont doarea_box"> <span>Hybridoma Generation and Screening</span> <div class="cont_area"> <ul class="ullist"> <li> At least 2 rounds of specific ELISA screening and 2 rounds of hybridoma subclone. </li> <li> Function-guaranteed hybridoma could be offered to client as an optional service after ELISA screening. </li> <li> FACS, ICC, IHC, WB, and flow cytometry assays are available for Hybridoma clone identification. </li> </ul> </div> </div> </div> </div> <div class="row textboxstyle-45 blue45"> <div class="col-md-12"> <div class="textboxstylecont doarea_box"> <span>Antibody Production and Purification</span> <div class="cont_area"> <p> Tailored to the specifications of our clients, antibody preparation and refinement, whether achieved <em>in vivo</em> or <em>in vitro</em>, will be meticulously executed. Delivery of top-tier antibodies of exceptional quality is assured. </p> </div> </div> </div> </div> <div class="row textboxstyle-45 blue45"> <div class="col-md-12"> <div class="textboxstylecont doarea_box"> <span>Additional options: <a href="/antibody-sequencing-service.html">Hybridoma Sequencing</a></span> <div class="cont_area"> <p> Sequencing (covering variable regions: VH and VL) and report (both DNA and amino acid sequences, with CDR regions highlighted). </p> </div> </div> </div> </div> <div class="row textboxstyle-45 blue45"> <div class="col-md-12"> <div class="textboxstylecont doarea_box"> <span>Delivery</span> <div class="cont_area"> <ul class="ullist"> <li> At least 1-3 hybridoma cells; </li> <li> 1-5mg antibody from one clone chosen by the client; </li> <li> Up to 20 hybridoma supernatants (500ul-1ml) for antibody function verification; </li> <li> Complete project report. </li> </ul> </div> </div> </div> </div> <div class="row textboxstyle-45 blue45"> <div class="col-md-12"> <div class="textboxstylecont doarea_box"> <span>Lead time</span> <div class="cont_area"> <p> 90-120 days. The time may vary depending on the different requirements, and additional time should be allowed for additional validation testing or repetition, if necessary. </p> </div> </div> </div> </div> <h2 class="h2_tit page_tab_margin" id="tab3"> Custom Antibody & Hybridoma Services </h2> <div class="row flex-box listbgover"> <div class="col-md-4"> <div class="border-box"> <a href="/Antibody-Production-by-Liposome-Immunization.html"><strong>Liposome Immunization</strong></a> <p> As a highly optimized service, our liposome immunization technology relies on a proprietary liposome immunization procedure using liposome-encapsulated antigens, which including but not limited to the polysaccharide, lipid, glycolipid, and DNA. </p> </div> </div> <div class="col-md-4"> <div class="border-box"> <a href="/monoclonal-antibody-production-rat-mouse.html"><strong>Murine Hybridoma</strong></a> <p> Our proprietary mouse immunization approach allows us to provide mouse monoclonal antibodies within 70 days. </p> </div> </div> <div class="col-md-4"> <div class="border-box"> <a href="/Rat-Hybridoma-Generation.html"><strong>Rat Hybridoma</strong></a> <p> We have developed a proprietary hybridoma-generation platform for efficient development of high-affinity and high-specificity rat monoclonal antibodies. </p> </div> </div> <div class="col-md-4"> <div class="border-box"> <a href="/Hamster-Hybridoma-Production.html"><strong>Hamster Hybridoma</strong></a> <p> We have extensive experience in producing monoclonal antibodies in hamsters. </p> </div> </div> <div class="col-md-4"> <div class="border-box"> <a href="/Guinea-Pig-Hybridoma-Production.htm"><strong>Guinea Pig Hybridoma</strong></a> <p> Through fusing with our specific myeloma as partner, guinea pig hybridoma can be developed with excellent stability and productivity. </p> </div> </div> <div class="col-md-4"> <div class="border-box"> <a href="/Hybridoma-Generation-in-Gene-Inactivated-Mice.html"><strong>Hybridoma Generation in Gene Inactivated Mice</strong></a> <p> The immunization of "knock-out" mice was an effective way to overcome the problem of inter-species sequence similarity in antibody production. </p> </div> </div> <div class="col-md-4"> <div class="border-box"> <a href="/anti-small-molecules-hybridoma-development.html"><strong>Anti-Small Molecules Hybridoma Development</strong></a> <p> We provide custom anti-small molecule mAb development services through hybridoma technology. </p> </div> </div> <div class="col-md-4"> <div class="border-box"> <a href="/function-guaranteed-antibody-development.html"><strong>Function-guaranteed Antibody Development</strong></a> <p> It is important to determine the application and hybridoma screening method since not all antibodies will work with every application. </p> </div> </div> <div class="col-md-4"> <div class="border-box"> <a href="/hybridoma-rescue-service.html"><strong>Hybridoma Rescue Service</strong></a> <p> We now have the capability and technology platforms to offer you diverse hybridoma rescue services. </p> </div> </div> <div class="col-md-4"> <div class="border-box"> <a href="/precision-antibody-replication-service.html"><strong>Precision Antibody Replication Service</strong></a> <p> Immunogen design service is one of our core technical services. We offer complementary protein expression or peptide design services to generate custom antibodies with better performance. </p> </div> </div> <div class="col-md-4"> <div class="border-box"> <a href="/bulk-antibody-production-services.html"><strong>Bulk Antibody Production Services</strong></a> <p> Delivering the final products with fast turnaround time and high quality. </p> </div> </div> <div class="col-md-4"> <div class="border-box"> <a href="/hybridoma-isotype-identification-and-switching-service.html"><strong>Hybridoma Isotype Identification and Switching Service</strong></a> <p> We provide world-class packaging services for hybridoma isotype Identification and switching. </p> </div> </div> </div> <h2 class="h2_tit page_tab_margin" id="tab4"> Characteristics of Hybridoma Services </h2> <ul class="ullist"> <li> Exceptional specificity, affinity, and precision; </li> <li> A wide array of species options; </li> <li> Capability to discern nuanced alterations (such as <a href="/Custom-antibody-services-for-post-translational-modification-specific-antibody-discovery.html">post-translational modifications</a>); </li> <li> Production of extensive arrays of functional antibodies; </li> <li> Access to thorough functional assessments; </li> <li> Identification of novel epitopes; </li> <li> Superior immunohistochemistry outcomes with formalin-fixed paraffin-embedded specimens; </li> <li> Remarkable outcomes for challenging antigens; </li> <li> Availability of high-throughput screening infrastructure; </li> </ul> <h2 class="h2_tit page_tab_margin" id="tab5"> Related Applications </h2> <p class="show-center"> <img loading="lazy" src="static/img/1-1-5-hybridoma-based-antibody-discovery-2.jpg" width="400" height="397" alt="Fig.2 Applications of our hybridoma services. (Creative Biolabs Original)" />Fig.2 Application of hybridoma services. </p> <ul class="ullist"> <li> <strong>Medical Diagnostics: Precision Instrumentation for Disease Detection</strong> </li> </ul> <p> The advent of hybridoma technology has transformed medical diagnostics, furnishing exceptionally precise mAb tailored for diverse assays. Employed in methodologies like ELISA, immunofluorescence, and Western blotting, these antibodies facilitate the identification of targeted proteins or pathogens within clinical specimens. This level of precision is crucial for facilitating early and accurate disease detection. </p> <ul class="ullist"> <li> <strong>Therapeutics: Precision Pharmacotherapy Enabled by MAb</strong> </li> </ul> <p> MAbs synthesized <em>via</em> hybridoma technology have emerged as indispensable components of therapeutic modalities. Whether addressing breast cancer or lymphoma, these antibodies are meticulously engineered to selectively target disease-associated molecules. The outcome is a therapeutic strategy characterized by minimized adverse effects and optimized efficacy. </p> <ul class="ullist"> <li> <strong>Foundational Investigation: Shedding Light on Cellular Enigmas</strong> </li> </ul> <p> Hybridoma technology has propelled fundamental inquiries in immunological, cellular, and associated disciplines. MAbs, serving as indispensable instruments, expedite the examination of distinct proteins, thereby elucidating cellular phenomena and unraveling the molecular intricacies inherent to diverse diseases. </p> <h2 class="h2_tit page_tab_margin" id="tab6"> Case Study </h2> <ul class="ullist"> <li> <em>Peptide Immunogen Design and Hybridoma Development</em> </li> </ul> <div class="row"> <div class="col-md-6"> <div class="serpic_list_unit"> <div class="imgarea"> <img src="../static/img/1-1-3-custom-antibody-hybridoma-services-2.jpg" width="380" height="251" loading="lazy" alt="Fig.3 2nd titration result-animal immunization. (Creative Biolabs Original)" /> </div> Fig. 3 2<sup>nd</sup> titration result-animal immunization. </div> </div> <div class="col-md-6"> <div class="serpic_list_unit"> <div class="imgarea"> <img src="../static/img/1-1-3-custom-antibody-hybridoma-services-3.jpg" width="380" height="238" loading="lazy" alt="Fig.4 ELISA screening of 2nd subclone. (Creative Biolabs Original)" /> </div> Fig. 4 ELISA screening of 2<sup>nd</sup> subclone. </div> </div> </div> <br /> <p> The client provided a protein target X, we designed a peptide immunogen and did the function test of selected positive hybridoma. Fig.2 is the ELISA titration result in the immunization process. Fig.3 is the ELISA result of the finally selected hybridomas. </p> <ul class="ullist"> <li> <em>Antibody Pair Development</em> </li> </ul> <div class="row"> <div class="col-md-6"> <div class="serpic_list_unit"> <div class="imgarea"> <img src="../static/img/1-1-3-custom-antibody-hybridoma-services-4.jpg" width="300" height="288" loading="lazy" alt="Fig.5 Reducing 12% SDS-PAGE analysis of purified recombinant protein. (Creative Biolabs Original)" /> </div> Fig. 5 Reducing 12% SDS-PAGE analysis of purified recombinant protein. </div> </div> <div class="col-md-6"> <div class="serpic_list_unit"> <div class="imgarea"> <img src="../static/img/1-1-3-custom-antibody-hybridoma-services-5.jpg" width="360" height="250" loading="lazy" alt="Fig.6 Antibody Pair Screening. (Creative Biolabs Original)" /> </div> Fig. 6 Antibody Pair Screening. </div> </div> </div> <br /> <p> As an industrial leader specializing in custom services, <strong>Creative Biolabs</strong> also provides gene-engineered antibodies including <a href="/sdab-single-domain-antibody.html">single-domain antibodies</a> and <a href="/Human-or-Humanized-Antibody-Services.html">humanized antibodies</a>. Moreover, we can conduct <a href="/phage-display-service.html">phage display antibody library screening</a> and <a href="/b-cell-sorting-platform.html">antibody development by the single B cell sorting platform</a>. Should you express interest in our offerings, we warmly encourage you to <a href="/contactus.html">reach out</a> for further elucidation. </p> <h2 class="h2_tit page_tab_margin" id="tab7"> Resources </h2> <p> Use the resources in our library to help you understand your options and make critical decisions for your study. </p> <div class="tab_nav"> <span class="tab_choosebg">Videos</span><span>Podcasts</span><span>Infographic</span> <span>Case study </span><span>Articles</span> </div>  <div class="tab_cont" style="display:block;"> <div class="row pagerow_pt"> <div class="col-md-4"> <div class="pagerow_pt_unit"> <img loading="lazy" src="static/img/video-video-camouse-fully-human-antibody-generation-platform.png" alt="CAMouse™-Fully Human Antibody Generation Platform" /> </div> <a href="https://youtu.be/hhY5nuYHeUs" target="_blank">CAMouse™-Fully Human Antibody Generation Platform</a> </div> <div class="col-md-4"> <div class="pagerow_pt_unit"> <img loading="lazy" src="static/img/video-development-of-monoclonal-antibodies.png" alt="Development of Monoclonal Antibodies" /> </div> <a href="https://youtu.be/O5_abD0eiIE" target="_blank">Development of Monoclonal Antibodies </a> </div> </div> </div>  <div class="tab_cont"> <div class="therapy_ad"> <a href="therapeutic-antibody.html"> <img loading="lazy" src="static/img/podcasts-ad.png" width="1700" height="500" srcset="../static/img/podcasts-ad.webp 1x,../static/img/podcasts-ad.webp 2x,../static/img/podcasts-ad.webp 3x" alt="In this brand new podcast series, we will show you everything you want to know about CAR-T therapy, including the mechanism, current applications, technology limitations, and potential strategies." /></a> </div> </div>  <div class="tab_cont"> <div class="row pagerow_pt"> <div class="col-md-4"> <div class="pagerow_pt_unit_2"> <img loading="lazy" src="/resource/images/Development-of-Monoclonal-Antibodies-1.jpg" width="600" height="500" alt="Development of Monoclonal Antibodies" /> </div> <a href="https://go.creative-biolabs.com/development-of-monoclonal-antibodies.html">Development of Monoclonal Antibodies</a> </div> <div class="col-md-4"> <div class="pagerow_pt_unit_2"> <img loading="lazy" src="/resource/images/A-Brief-Introduction-to-Antibodies-1.jpg" width="600" height="500" alt="A Brief Introduction to Antibodies" /> </div> <a href="https://go.creative-biolabs.com/a-brief-introduction-to-antibodies.html">A Brief Introduction to Antibodies</a> </div> <div class="col-md-4"> <div class="pagerow_pt_unit_2"> <img loading="lazy" src="/resource/images/Antibody-Based-Therapies-1.jpg" width="600" height="500" alt="CAR-T Cell Immunotherapy: Development of CAR-T Cells" /> </div> <a href="https://go.creative-biolabs.com/antibody-based-therapies.html">Antibody-Based Therapies</a> </div> <div class="col-md-4"> <div class="pagerow_pt_unit_2"> <img loading="lazy" src="/resource/images/B-Cell-Sorting-Platform-Creative-Biolabs.jpg" width="600" height="500" alt="B-Cell Sorting Platform" /> </div> <a href="https://go.creative-biolabs.com/b-cell-sorting-platform.html">B-Cell Sorting Platform</a> </div> </div> </div>    <div class="tab_cont"> <div class="row pagerow_pt"> <div class="col-md-4"> <div class="pagerow_pt_unit_2"> <img loading="lazy" src="/resource/images/Antibody-Development-Case-Study-com.jpg" width="530" height="500" alt="Antibody Development Case Study" /> </div> <a href="https://go.creative-biolabs.com/antibody-development-case-study.html">Antibody Development Case Study</a> </div> </div> </div>    <div class="tab_cont"> <div class="row pagerow_pt"> <div class="col-md-4"> <div class="pagerow_pt_unit"> <img loading="lazy" src="static/img/Hybridoma-Overview-type.jpg" alt="Hybridoma Overview" /> </div> <a href="hybridoma-overview.html">Hybridoma Overview</a> </div> <div class="col-md-4"> <div class="pagerow_pt_unit"> <img loading="lazy" src="static/img/protocol-1.png" width="600" height="500" alt="Therapeutic Monoclonal Antibody Therapies in Chronic Autoimmune Demyelinating Neuropathies" /> </div> <a href="therapeutic-monoclonal-antibody-therapies-in-chronic-autoimmune-demyelinating-neuropathies.html">Therapeutic Monoclonal Antibody Therapies in Chronic Autoimmune Demyelinating Neuropathies</a> </div> <div class="col-md-4"> <div class="pagerow_pt_unit"> <img loading="lazy" src="static/img/Development-of-Human-Monoclonal-Antibody-for-Claudin-3-Overexpressing-Carcinoma-Targeting_pic.png" width="600" height="500" alt="Development of Human Monoclonal Antibody for Claudin-3 Overexpressing Carcinoma Targeting" /> </div> <a href="development-of-human-monoclonal-antibody-for-claudin-3-overexpressing-carcinoma-targeting.html">Development of Human Monoclonal Antibody for Claudin-3 Overexpressing Carcinoma Targeting</a> </div> </div> </div> <script> window.addEventListener('load',function(){ // $(function () { $(".tab_nav span").click(function(){ var eqindex=$(this).index(); 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