Single Domain Antibody (SdAb) Discovery Services

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With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects. </p>  <b class="Highlights_tit">Highlights</b> <ul class="HighlightsArea"> <li class="col-md-6 col-lg-6 col-sm-6"> <img src="static/images/Solutions-menu-lights-pic-1.jpg" srcset="static/images/Solutions-menu-lights-pic-1.webp 1x" width="201" height="131" loading="lazy"><a href="discovery-services.html" class="bannerbutton"><i class="fa fa-angle-right"></i>Discovery Services</a> </li> <li class="col-md-6 col-lg-6 col-sm-6"> <img src="static/images/Solutions-menu-lights-pic-2.jpg" srcset="static/images/Solutions-menu-lights-pic-2.webp 1x" width="201" height="131" loading="lazy"><a href="engineering-services.html" class="bannerbutton"> <i class="fa fa-angle-right"></i>Engineering Services </a> </li> </ul> </li> </ul> </div> </div> </li> <li> <span>Characterization</span> <div class="secondMenu"> <div class="second-menu-area-box"> <ul style="margin-top:0;">  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We are committed to providing highly customized comprehensive solutions with the best quality to advance your projects. </p> <b class="Highlights_tit">Highlights</b> <ul class="HighlightsArea"> <li class="col-md-6 col-lg-6 col-sm-6"> <img src="static/images/Abouts-menu-lights-pic-1.jpg" srcset="static/images/Abouts-menu-lights-pic-1.webp 1x" width="200" height="131" loading="lazy"><a href="aboutus.html" class="bannerbutton"> <i class="fa fa-angle-right"></i>About CBL </a> </li> <li class="col-md-6 col-lg-6 col-sm-6"> <img src="static/images/Abouts-menu-lights-pic-2.jpg" srcset="static/images/Abouts-menu-lights-pic-2.webp 1x" width="200" height="131" loading="lazy"><a href="contactus.html" class="bannerbutton"> <i class="fa fa-angle-right"></i>Contact Us </a> </li> </ul> </li> </ul> </div> </div> </li> </ul> <script> window.addEventListener('load',function(){ var arr = ['/drug-discovery/therapeutics/','/bioconjugation/','/adc/']; $('.mennav ul li a').each(function(){ var $this = $(this); var href = $this.attr("href").toLocaleLowerCase(); $.each(arr, function(index, value) { if (href && href.startsWith(value)) { $this.attr('target', '_blank'); } }); }); }); </script> </div> <div class="top-search search-group"> <div class="col-md-5 col-sm-6"> <label class="radio-inline"><input type="radio" name="search" class="rdo_productsearch" value="product" data-type="product" placeholder="Please input cat#, product name, abbr, or keywords."> Product</label> <label class="radio-inline"><input type="radio" name="search" class="rdo_servicesearch" value="service" data-type="service" placeholder="Please input your keywords."> Service</label> <label class="radio-inline"><input type="radio" name="search" class="rdo_googlesearch" value="google" data-type="google" placeholder="Please input your keywords." checked="checked"> Keyword</label> </div> <div class="col-md-7 col-sm-6 search-div"> <input id="keys" name="keys" placeholder="Please input your keywords." class="search-input"> <div class="selectItems"></div> <span class="search_btn"><i class="fa fa-search"></i></span> </div> </div> </div> <div class="searchShow"> <div class="searchtopbg"></div> <span class="searchclosed"><i class="fa fa-times"></i>Close</span> <div class="addsearch search-group"></div> </div> <div class="banner_inpage services_bg_2"> <div class="auto-container small_content-width about_container"> <ul class="bread-crumb clearfix"> <li><a href="/">Home</a></li> <li><a href="javascrpt:void(0)">Discovery</a></li><li><a href="javascrpt:void(0)">By Binder</a></li> <li>Single Domain Antibody (SdAb) Discovery</li> </ul> <p>"Creative Biolabs is committed to providing highly customized comprehensive solutions with the best quality to advance our global clients’ projects."</p> </div> </div> <script> window.addEventListener('load', function () { if (($('.bread-crumb li:last-child').text()) == "Peer-Reviewed Publications") { $(".banner_inpage").addClass("peer-reviewed-publications_bg").removeClass("services_bg_2"); } if ($(window).hasClass('bread-crumb')) { if ($(".bread-crumb").html().indexOf("RESOURCE") > -1) { $(".pronote").hide(); } if ($(".bread-crumb").html().indexOf("Careers") > -1) { $(".pronote").hide(); } } }); </script> <div class="small_content-width services-container"> <div class="col-md-9"> <div class="inpage_section_title"> <h1>Single Domain Antibody (SdAb) Discovery Services</h1> </div> <div class="page-tab"> <span onclick="window.location.href=window.location.pathname+'#tab1'">SdAb Discovery</span> <span onclick="window.location.href=window.location.pathname+'#tab2'">Workflow </span> <span onclick="window.location.href=window.location.pathname+'#tab3'">Custom Development </span> <span onclick="window.location.href=window.location.pathname+'#tab4'">Platforms</span> <span onclick="window.location.href=window.location.pathname+'#tab5'">Features</span> <span onclick="window.location.href=window.location.pathname+'#tab6'">Schematic Outcomes</span> <span onclick="window.location.href=window.location.pathname+'#tab7'">Publications</span> <span onclick="window.location.href=window.location.pathname+'#tab8'">FAQ</span> <span onclick="window.location.href=window.location.pathname+'#tab9'">Resources</span> </div> <script> window.addEventListener('load',function(){ page_tab_margin() page_tab_css(); })</script> <h2 class="h2_tit page_tab_margin" id="tab1"> One-Stop Solution for Single Domain Antibody (SdAb) Discovery </h2> <p> Leveraging our cutting-edge platforms, Creative Biolabs specializes in the discovery of single domain antibodies (sdAbs) with high affinity and specificity. Based on our clients' requirements, we could immunize camels, llamas, alpacas, or even sharks as repertoire source to develop single domain antibodies targeting various antigens. </p> <p> Alternatively, our team can screen our <a href="Premade-single-domain-antibody-library.html">Premade Single Domain Antibody (SdAb) Libraries</a> to isolate high-potency single domain antibodies against a large number of challenge targets. In addition to our sdAb discovery services, we offer a comprehensive range of <a href="/sdab/products.htm">Recombinant Single Domain Antibody (SdAb) Products</a> to meet all your needs. </p> <p class="ServiceShowPic"> <img loading="lazy" src="../static/img/single-domain-antibody-sdAb-discovery-services-1.jpg" alt="Fig. 1 A figure shows single domain antibodies from different camelid species. (Creative Biolabs Original)" width="700" height="309" /> </p> <h2 class="h2_tit page_tab_margin" id="tab2"> Workflow for SdAb Discovery at Creative Biolabs </h2> <div class="row flow-chart doarea_box"> <div class="col-md-6"> <div class="fcunit"> <span><b>01</b>Consultation and Project Planning with Us</span> <p> Expert guidance in project design, target selection, and strategy development to meet specific project requirements. </p> <p class="doarea"> <span>~2 weeks</span> <span class="inquiry-button">Inquire to Us</span> </p> </div> </div> <div class="col-md-6"> <div class="fcunit"> <span><b>02</b>Antigen Design & Preparation</span> <p> Design and production of antigens tailored to a wide range of targets such as large protein molecules, <a href="sdab-discovery-against-membrane-proteins-by-dna-immunization.html">membrane proteins</a>, haptens, or other challenging targets, ensuring optimal antibody generation. </p> <p class="doarea"> <span>~6 weeks</span> <span class="inquiry-button">Inquire to Us</span> </p> </div> </div> <div class="col-md-6"> <div class="fcunit"> <span><b>03</b><a href="single-domain-antibody-library-service.html">SdAb Library Construction & Screening</a></span> <p> Comprehensive options are available at Creative Biolabs to initiate your sdAb discovery projects. </p> <ul class="ullist"> <li> Immune sdAb library construction gains the highest target specificity from llamas, alpacas, or camels. </li> <li> Synthetic sdAb library construction endows outstanding repertoire diversity and custom unique design. </li> <li> <a href="Premade-single-domain-antibody-library.html">Premade sdAb library licensing</a> offers a fast-track scheme towards <a href="premade-single-domain-antibody-library-screening-service.html">screening & validation</a>. </li> </ul> <p> High-throughput library screening & validation will be conducted with the most suitable strategy to select candidate sdAbs with desired properties, such as specificity, affinity, stability, or functionality. </p> <p class="doarea"> <span>1-4 months</span> <span class="inquiry-button">Inquire to Us</span> </p> </div> </div> <div class="col-md-6"> <div class="fcunit"> <span><b>04</b>Specific SdAb Characterization</span> <p> Various characterization assays of sdAbs upon your request to ensure suitability for intended properties: </p> <ul class="ullist"> <li> Affinity Measurement </li> <li> Specificity Assessment </li> <li> Stability Testing (<em>e.g.</em>, Thermal Stability, pH Stability). </li> <li> Functional Assays (<em>e.g.</em>, Cell Binding, Neutralizing). </li> <li> Epitope Mapping. </li> <li> <a href="/form/order.aspx?n=Specific SdAb Characterization">More</a> </li> </ul> <p class="doarea"> <span class="inquiry-button">Inquire to Us</span> </p> </div> </div> <div class="col-md-6"> <div class="fcunit"> <span><b>05</b>SdAb Engineering & Optimization</span> <p> Through tailored strategies to optimize sdAbs with desired properties for specific applications (<em>e.g.</em>, therapeutics, diagnostics, or research), which including but not limited to: </p> <ul class="ullist"> <li> <a href="sdAb-Affinity-Maturation.html">SdAb Affinity Maturation</a> </li> <li> <a href="Human-or-Humanized-sdAb-Production.html">SdAb Humanization</a> </li> <li> <a href="Bispecific-sdAb-Production.html">SdAb Bispecific Engineering</a> </li> <li> <a href="single-domain-antibody-conjugation-services.html">SdAb Conjugation</a> </li> </ul> <p class="doarea"> <span class="inquiry-button">Inquire to Us</span> </p> </div> </div> <div class="col-md-6"> <div class="fcunit"> <span><b>06</b>Pilot Expression and Scale-Up Production</span> <p> Various expression systems, such as bacteria, yeast, or mammalian cells, are set up by Creative Biolabs to express and purify sdAbs with high purity and activity. Meanwhile, our scalable production system offers flexible solutions to meet your specific demands on scaling up sdAb manufacture. </p> <p class="doarea"> <span>6 weeks</span> <span class="inquiry-button">Inquire to Us</span> </p> </div> </div> </div> <h2 class="h2_tit page_tab_margin" id="tab3"> Highly Customized Solutions for SdAb Development Towards Various Applications </h2> <div class="row"> <div class="col-md-4 col-sm-6"> <div class="featuresunit"> <strong class="featurestit"> <img loading="lazy" src="../static/img/single-domain-antibody-sdAb-discovery-services-2.jpg" width="120" height="120" alt="Blood-Brain Barrier (BBB) Specific SdAb" /><br /> <a href="sdAb-Discovery-against-Membrane-Proteins-by-DNA-Immunization.html">Membrane Protein Specific SdAb</a></strong> </div> </div> <div class="col-md-4 col-sm-6"> <div class="featuresunit"> <strong class="featurestit"> <img loading="lazy" src="../static/img/single-domain-antibody-sdAb-discovery-services-3.jpg" width="120" height="120" alt="Blood-Brain Barrier (BBB) Specific SdAb" /><br /> <a href="Camel-Anti-Idiotypic-Antibodies-sdAbs-Production-service.html">Idiotype Specific SdAb</a></strong> </div> </div> <div class="col-md-4 col-sm-6"> <div class="featuresunit"> <strong class="featurestit"> <img loading="lazy" src="../static/img/single-domain-antibody-sdAb-discovery-services-4.jpg" width="120" height="120" alt="Blood-Brain Barrier (BBB) Specific SdAb" /><br /> <a href="Anti-BBB-sdAb-Discovery-Service.html">Blood-Brain Barrier (BBB) Specific SdAb</a></strong> </div> </div> <div class="col-md-4 col-sm-6"> <div class="featuresunit"> <strong class="featurestit"> <img loading="lazy" src="../static/img/single-domain-antibody-sdAb-discovery-services-5.jpg" width="120" height="120" alt="Blood-Brain Barrier (BBB) Specific SdAb" /><br /> <a href="anti-albumin-single-domain-antibody-discovery-service.html">Albumin Specific SdAb</a></strong> </div> </div> <div class="col-md-4 col-sm-6"> <div class="featuresunit"> <strong class="featurestit"> <img loading="lazy" src="../static/img/single-domain-antibody-sdAb-discovery-services-6.jpg" width="120" height="120" alt="Blood-Brain Barrier (BBB) Specific SdAb" /><br /> <a href="intrabody-discovery-service.html">SdAb based Intrabody</a></strong> </div> </div> </div> <h2 class="h2_tit page_tab_margin" id="tab4"> Platforms for SdAb Discovery </h2> <div class="row textboxstyle-45 blue45"> <div class="col-md-4"> <div class="textboxstylecont doarea_box"> <span>Phage Display Platform</span> <div class="cont_area"> <p> Phage display has emerged as a powerful tool for the discovery and optimization of sdAbs. This <em>in vitro</em> selection technique leverages bacteriophages - viruses that infect bacteria - to present sdAbs on their surface. By linking the displayed sdAb, phage display enables the rapid identification of high-affinity sdAbs from vast libraries of variants. </p> <p class="doarea"> <span class="inquiry-button">Learn More</span> </p> </div> </div> </div> <div class="col-md-4"> <div class="textboxstylecont doarea_box"> <span>Yeast Display Platform</span> <div class="cont_area"> <p> Yeast display allows efficient screening and selection of high-affinity sdAb binders against cell line targets. The eukaryotic nature of yeast enables proper folding and post-translational modifications of the displayed binders, which is crucial for maintaining the functionality of sdAbs. </p> <p class="doarea"> <span class="inquiry-button">Learn More</span> </p> </div> </div> </div> <div class="col-md-4"> <div class="textboxstylecont doarea_box"> <span>Single B Cell Sorting Platform</span> <div class="cont_area"> <p> B cell repertoire is a potential source of sdAbs. Single B cell sorting technology has advanced significantly recently, enhancing efficiency and feasibility in rapidly obtaining specific sdAbs. Our cutting-edge Native™ platform encompasses B cell screening, cloning, and in vitro expression, and offers an innovative solution for discovering and developing novel sdAbs. </p> <p class="doarea"> <span class="inquiry-button">Learn More</span> </p> </div> </div> </div> </div> <h2 class="h2_tit page_tab_margin" id="tab5"> Features of Our Service </h2> <div class="row"> <div class="col-md-4 col-sm-6"> <div class="featuresunit"> <strong class="featurestit"> <img loading="lazy" src="../static/img/single-domain-antibody-sdAb-discovery-services-11.png" width="120" height="120" alt="Blood-Brain Barrier (BBB) Specific SdAb" /><br /> Specialized Expertise</strong> <div class="featurescont"> Our team comprises seasoned experts dedicated to sdAb development who possess the knowledge and skills to deliver exceptional results. </div> </div> </div> <div class="col-md-4 col-sm-6"> <div class="featuresunit"> <strong class="featurestit"> <img loading="lazy" src="../static/img/single-domain-antibody-sdAb-discovery-services-12.png" width="120" height="120" alt="Blood-Brain Barrier (BBB) Specific SdAb" /><br /> Tailored One-Stop Solutions</strong> <div class="featurescont"> Whether you need sdAbs for research, diagnostics, or therapeutics, we work closely with you to develop strategies that align with your goals and offer customized one-stop solutions from antigen design and library construction to sdAb engineering and scale-up production. </div> </div> </div> <div class="col-md-4 col-sm-6"> <div class="featuresunit"> <strong class="featurestit"> <img loading="lazy" src="../static/img/single-domain-antibody-sdAb-discovery-services-13.png" width="120" height="120" alt="Blood-Brain Barrier (BBB) Specific SdAb" /><br /> Cutting-Edge Technology</strong> <div class="featurescont"> By employing the latest advancements in antibody discovery, screening, and characterization, we are confident to ensure the highest quality sdAbs with superior performance characteristics. </div> </div> </div> <div class="col-md-4 col-sm-6"> <div class="featuresunit"> <strong class="featurestit"> <img loading="lazy" src="../static/img/single-domain-antibody-sdAb-discovery-services-14.png" width="120" height="120" alt="Blood-Brain Barrier (BBB) Specific SdAb" /><br /> Efficient Project Management</strong> <div class="featurescont"> Our efficient project management processes and streamlined workflows enable us to provide support exactly covering all stages of sdAb development, allowing you to focus on advancing your projects without delay. </div> </div> </div> <div class="col-md-4 col-sm-6"> <div class="featuresunit"> <strong class="featurestit"> <img loading="lazy" src="../static/img/single-domain-antibody-sdAb-discovery-services-15.png" width="120" height="120" alt="Blood-Brain Barrier (BBB) Specific SdAb" /><br /> Collaborative Partnership</strong> <div class="featurescont"> We have helped numerous clients achieve their research or therapeutic goals and will work closely with you, providing regular updates, insights, and guidance to ensure the success of your project. </div> </div> </div> <div class="col-md-4 col-sm-6"> <div class="featuresunit"> <strong class="featurestit"> <img loading="lazy" src="../static/img/single-domain-antibody-sdAb-discovery-services-16.png" width="120" height="120" alt="Blood-Brain Barrier (BBB) Specific SdAb" /><br /> Confidentiality and Integrity</strong> <div class="featurescont"> We uphold the highest standards of confidentiality and integrity, safeguarding your proprietary information and respecting the confidentiality of your projects at all times. </div> </div> </div> </div> <h2 class="h2_tit page_tab_margin" id="tab6"> Schematic Outcomes for Typical SdAb Discovery Projects </h2> <div class="row pagerow_pt sitealign"> <div class="col-md-3 col-sm-6"> <div class="pagerow_pt_unit"> <img loading="lazy" src="../static/img/single-domain-antibody-sdAb-discovery-services-7.png" height="700" alt="Fig. 2 A good titration results from llama immunization. (Creative Biolabs Original)" /> </div> <p> Llama Immunization with Good Response </p> </div> <div class="col-md-3 col-sm-6"> <div class="pagerow_pt_unit"> <img loading="lazy" src="../static/img/single-domain-antibody-sdAb-discovery-services-8.png" height="700" alt="Fig. 3 An excellent PCR results prove an immune sdAb library is constructed with high accuracy. (Creative Biolabs Original)" /> </div> <p> Constructed SdAb Library with High Accuracy </p> </div> <div class="col-md-3 col-sm-6"> <div class="pagerow_pt_unit"> <img loading="lazy" src="../static/img/single-domain-antibody-sdAb-discovery-services-9.jpg" height="700" alt="Fig. 4 A pair of histogram revealed positive sdAb binders have been enriched properly through library screening against target protein. (Creative Biolabs Original)" /> </div> <p> Positive SdAb Binders Enrichment </p> </div> <div class="col-md-3 col-sm-6"> <div class="pagerow_pt_unit"> <img loading="lazy" src="../static/img/single-domain-antibody-sdAb-discovery-services-10.jpg" height="700" alt="Fig. 5 A SDS-PAGE results and a ELISA based line chart revealed successful sdAb production with high purity and good affinity. (Creative Biolabs Original)" /> </div> <p> High-Quality SdAb Production </p> </div> </div> <script type="text/javascript"> window.addEventListener('load',function(){ spage_showbigpic(".pagerow_pt"); }); </script> <h3 class="h3_tit"> Get Started </h3> <p> Ready to harness the power of single domain antibodies for your research or therapeutic development? <span class="inquiry-button">Contact us</span> today to discuss your project requirements and explore how our services can accelerate your success. </p> <h2 class="h2_tit page_tab_margin" id="tab7"> Acknowledged Publications </h2> <div class="contheight"> <ol class="ollist"> <li> Ma, Lin, <em>et al.</em> "Generation and characterization of a human nanobody against VEGFR-2." <em>Acta Pharmacologica Sinica</em> 37.6 (2016): 857-864. </li> <li> Doshi, Rupak, <em>et al.</em> "<em>In vitro</em> nanobody discovery for integral membrane protein targets." <em>Scientific reports</em> 4.1 (2014): 6760. </li> <li> Kim, Dae Young, <em>et al.</em> "Mutational approaches to improve the biophysical properties of human single-domain antibodies." <em>Biochimica et Biophysica Acta (BBA)-Proteins and Proteomics</em> 1844.11 (2014): 1983-2001. </li> <li> Chatterjee, Shilpak, <em>et al.</em> "CD38-NAD+ axis regulates immunotherapeutic anti-tumor T cell response." <em>Cell metabolism</em> 27.1 (2018): 85-100. </li> <li> Suratt, Benjamin T. "Suppression of leptin action for treatment of pulmonary infections." U.S. Patent No. <a name="OLE_LINK5"></a><a name="OLE_LINK6"></a>20,150,329,635. Nov 19, 2015. </li> <li> Sunanda SINGH. “Single domain antibodies directed against intracellular antigens.” WO2016065323 A2. Apr 28, 2016. </li> <li> Sunanda SINGH. “Single domain antibodies directed against tnfalpha.” US20160115226 A1. Apr 28, 2016. </li> <li> Sunanda SINGH. “Single domain antibodies directed against kras.” US20160115244 A1. Apr 28, 2016. </li> <li> Sunanda SINGH. “Single domain antibodies directed against intracellular antigens.” US20160115247 A1. Apr 28, 2016. </li> <li> Sunanda SINGH. “Single domain antibodies directed against intracellular antigens.” US20170327598A1. Nov 16, 2017. </li> <li> Sunanda SINGH. “Single domain antibodies directed against stat3.” US20160115248 A1. Apr 28, 2016. </li> <li> Sunanda SINGH. “Single domain antibodies directed against intracellular antigens.” WO2016065323 A3. Jun 16, 2016. </li> <li> Sunanda SINGH. “Single domain antibodies directed against intracellular antigens.” US20170267784A1. Sep 21, 2017. </li> <li> Jerry Clifford, Ben Thompson. A method of determining the abundance of a target molecule in a sample. WO2017103210 A1. Jun 22, 2017 </li> <li> Paul C. Lee. “Nanoparticle probes and methods of making and use thereof.” US20170269095A1. Sep 21, 2017. </li> <li> Paul C. Lee. “Nanoparticle probes and methods of making and use thereof.” US20180000972A1. Jan 04, 2018. </li> </ol> </div> <script type="text/javascript"> window.addEventListener('load', function () { // $(function () { moreinfo_("sym_more","contheight"); // }); }); </script> <div class="sym_more"> <span class="">More Infomation</span> </div> <script type="text/javascript"> window.addEventListener('load',function(){ faqlist(6); }); </script> <h2 class="h2_tit page_tab_margin" id="tab8"> Frequently Asked Questions (FAQ) </h2> <ol class="ollist faqlist"> <li> <span class="sFAQTit"><strong>Q:</strong> What is Single Domain Antibody?</span> <p> <strong>A:</strong> Single domain antibody (sdAb), also known as a domain antibody, V<sub>H</sub>H, or V<sub>NAR</sub>, is a kind of antibody fragment consisting of a single monomeric variable antibody domain. It lacks the light chain and the CH domain of the heavy chain found in the conventional Fab region. With a molecular weight of only 12-15 kDa, it is significantly smaller than a full-length antibody (150-160 kDa) or other antibody fragments such as Fab (~50 kDa) and scFv (~25 kDa). </p> <p> Single domain antibodies represent a powerful class of molecules with unique properties that make them valuable tools for biomedical research and therapeutic development. Their versatility, stability, and specificity have led to their widespread adoption in numerous applications, driving innovation in various fields of science and medicine. </p> </li> <li> <span class="sFAQTit"><strong>Q:</strong> What advantages do sdAbs offer over conventional antibodies?</span> <p> <strong>A:</strong> sdAbs have several advantages, including small size, high stability, and solubility, and the ability to target unique epitopes with high specificity. They can target epitopes inaccessible to conventional antibodies and have the potential for oral delivery and tissue penetration. Additionally, sdAbs can be easily engineered for various applications. </p> </li> <li> <span class="sFAQTit"><strong>Q:</strong> How are single domain antibodies generated?</span> <p> <strong>A:</strong> Single domain antibodies can be generated with immunization of camelids (e.g., llamas, camels) or directly screen sdAbs with desired properties from a synthetic library. These methods involve isolating sdAbs with high affinity and specificity for a target antigen from diverse antibody repertoires coupled with display technologies such as phage display or yeast display. </p> </li> <li> <span class="sFAQTit"><strong>Q:</strong> What are the primary applications of single domain antibodies? </span> <p> <strong>A:</strong> Single domain antibodies have diverse applications across research, diagnostics, therapeutics, and biotechnology. They can be used for target validation, imaging, drug delivery, protein purification, and also as research reagents. Their small size and unique properties make them valuable tools in various fields. </p> </li> <li> <span class="sFAQTit"><strong>Q:</strong> What are the challenges in the production and development of single domain antibodies? </span> <p> <strong>A:</strong> Although single domain antibodies have many advantages in drug development, their production and development processes also face some challenges. The first is to ensure the high affinity and specificity of sdAbs, which is very important for their effectiveness. Therefore, through fine engineering design and screening, it is necessary to develop single domain antibodies with high affinity and high specificity. In addition, single domain antibodies usually have good tissue permeability and stability. However, in practical applications, the half-life of sdAbs is short, which limits their effectiveness in some therapeutic applications. To overcome this problem, researchers are developing strategies such as binding to long-acting serum albumin or prolonging its half-life in the body by changing its molecular structure. Finally, how to achieve high yield expression and purification of single domain antibodies is still a technical challenge. This requires meticulous process development and optimization to ensure that the produced single domain antibodies have the required purity, activity, and safety. </p> </li> <li> <span class="sFAQTit"><strong>Q:</strong> How to ensure high affinity and specificity in the development of single domain antibodies?</span> <p> <strong>A:</strong> The common method is to use phage display technology or other surface display technology. Through these techniques, single domain antibodies with high affinity and specificity for specific antigens can be screened from a large antibody library. In addition, directed evolution is another strategy to enhance the affinity of sdAbs. By mutating the key amino acids of sdAbs, a large number of variants are generated, from which the variants with higher affinity for specific antigens can be screened. This process can be repeated to gradually improve the affinity of single domain antibodies. The antibody can also be humanized by molecular engineering technology to reduce immunogenicity while maintaining its affinity and specificity. This is particularly important for the development of single domain antibodies for clinical treatment. </p> </li> <li> <span class="sFAQTit"><strong>Q:</strong> What services do you provide for single domain antibodies? </span> <p> <strong>A:</strong> Our services encompass the entire process of single domain antibody development, from antigen selection, animal immunization, library construction, to screening, characterization, affinity maturation, and scale-up production. We also offer customized services tailored to specific project needs. </p> </li> <li> <span class="sFAQTit"><strong>Q:</strong> What species are used for sdAb generation? </span> <p> <strong>A:</strong> We primarily utilize llamas, alpacas, camels, or synthetic libraries for sdAb discovery. However, we are flexible and can discuss alternative options based on project requirements and client preferences. </p> </li> <li> <span class="sFAQTit"><strong>Q:</strong> What is the typical timeline for single domain antibody development?</span> <p> <strong>A:</strong> The timeline varies depending on the specific project requirements and factors such as the complexity of the target, the desired affinity, and the selected method of generation. Typically, the process can range from a few months for discovery to additional time for optimization and production. We work closely with clients to establish timelines that meet their needs. </p> </li> <li> <span class="sFAQTit"><strong>Q:</strong> Can you produce single domain antibodies against difficult targets?</span> <p> <strong>A:</strong> We specialize in generating sdAbs against challenging targets, including small molecules, peptides, membrane proteins, and highly conserved epitopes. Our team has extensive experience in target validation and optimization to tackle challenging antigens. </p> </li> <li> <span class="sFAQTit"><strong>Q:</strong> How can I initiate a project with your single domain antibody services?</span> <p> <strong>A:</strong> To start a project or inquire further about our single domain antibody services, please contact our team, through online inquiry box, email info@creative-biolabs.com, or call +1-631-416-1478 directly, with details about your requirements, including the target antigen, intended applications, timeline, and any specific customization needs. We look forward to collaborating with you to achieve your goals. </p> </li> </ol> <h2 class="h2_tit page_tab_margin" id="tab9"> Resources </h2> <p> Use the resources in our library to help you understand your options and make critical decisions for your study. </p> <div class="tab-box-con"> <div class="tab_nav"> <span class="tab_choosebg">Videos</span><span>Podcasts</span><span>Infographic</span><span>Flyer</span> <span>Case study </span><span>Brochures</span> </div>  <div class="tab_cont" style="display:block;"> <div class="row pagerow_pt"> <div class="col-md-4"> <div class="pagerow_pt_unit"> <img loading="lazy" src="../static/img/a-potent-sars-cov-2-neutralizing-sdab.png" alt="Webinar: A Potent SARS-CoV-2 Neutralizing SdAb" /> </div> <a href="https://youtu.be/TOnpBGaV6RE" target="_blank">Webinar: A Potent SARS-CoV-2 Neutralizing SdAb</a> </div> <div class="col-md-4"> <div class="pagerow_pt_unit"> <img loading="lazy" src="../static/img/video-phage-display-technology.png" alt="Phage Display Technology" /> </div> <a href="https://youtu.be/mczJ3ZbpVW8" target="_blank">Phage Display Technology</a> </div> <div class="col-md-4"> <div class="pagerow_pt_unit"> <img loading="lazy" src="../static/img/video-development-of-monoclonal-antibodies-s.png" alt="Antibody Library Screening" /> </div> <a href="https://youtu.be/NGqipRkxXiY" target="_blank">Antibody Library Screening</a> </div> </div> </div>  <div class="tab_cont"> <div class="therapy_ad"> <a href="therapeutic-antibody.html"> <img loading="lazy" src="../static/img/podcasts-ad.png" width="1700" height="500" srcset="../static/img/podcasts-ad.webp 1x,../static/img/podcasts-ad.webp 2x,../static/img/podcasts-ad.webp 3x" alt="In this brand new podcast series, we will show you everything you want to know about CAR-T therapy, including the mechanism, current applications, technology limitations, and potential strategies." /></a> </div> </div>  <div class="tab_cont"> <div class="row pagerow_pt"> <div class="col-md-4"> <div class="pagerow_pt_unit_2"> <img loading="lazy" src="/resource/images/paghe-display-1.jpg" width="600" height="500" alt="Phage Display Technology" /> </div> <a href="https://go.creative-biolabs.com/phage-display-infographic.html">Phage Display Technology</a> </div> <div class="col-md-4"> <div class="pagerow_pt_unit_2"> <img loading="lazy" src="/resource/images/Ribosome-Display-1.jpg" width="600" height="500" alt="Ribosome Display Technology" /> </div> <a href="https://go.creative-biolabs.com/ribosome-display-infographic.html">Ribosome Display Technology</a> </div> <div class="col-md-4"> <div class="pagerow_pt_unit_2"> <img loading="lazy" src="/resource/images/Yeast-Display-Infographic-1.jpg" width="600" height="500" alt="Yeast Display Technology" /> </div> <a href="https://go.creative-biolabs.com/yeast-display-infographic.html">Yeast Display Technology</a> </div> <div class="col-md-4"> <div class="pagerow_pt_unit_2"> <img loading="lazy" src="/resource/images/Bacterial-Display-Creative-Biolabs.jpg" width="600" height="500" alt="A Brief Introduction to Antibodies" /> </div> <a href="https://go.creative-biolabs.com/bacterial-display-infographic.html">Bacterial Display Technology</a> </div> <div class="col-md-4"> <div class="pagerow_pt_unit_2"> <img loading="lazy" src="/resource/images/Antibody-Library-Screening-Creative-Biolabs.jpg" width="600" height="500" alt="Antibody Library Screening" /> </div> <a href="https://go.creative-biolabs.com/antibody-library-screening.html">Antibody Library Screening</a> </div> <div class="col-md-4"> <div class="pagerow_pt_unit_2"> <img loading="lazy" src="/resource/images/antibody-humanization-1.jpg" width="600" height="500" alt="Antibody Humanization" /> </div> <a href="https://go.creative-biolabs.com/antibody-humanization-infographic.html">Antibody Humanization</a> </div> </div> </div>  <div class="tab_cont"> <div class="row pagerow_pt"> <div class="col-md-4"> <div class="pagerow_pt_unit_2"> <img loading="lazy" src="/resource/images/Domain-Antibody-Engineering-Discovery-1.jpg" width="600" height="500" alt="Phage Display Technology in Creative Biolabs" /> </div> <a href="/resource/pdf/com/flyers/Flyer-sdAb-Discovery-and-Engineering.pdf">Single Domain Antibody Engineering & Discovery</a> </div> </div> </div>  <div class="tab_cont"> <div class="row pagerow_pt"> <div class="col-md-4"> <div class="pagerow_pt_unit_2"> <img loading="lazy" src="/resource/sdab/images/Case-Study-4.jpg" width="530" height="500" alt="sdAb Development for Soluble Protein Target" /> </div> <a href="https://go.creative-biolabs.com/sdab-development-for-soluble-protein-target.html">sdAb Development for Soluble Protein Target</a> </div> <div class="col-md-4"> <div class="pagerow_pt_unit_2"> <img loading="lazy" src="/resource/sdab/images/Case-Study-5.jpg" width="530" height="500" alt="sdAb Development for Hapten Target" /> </div> <a href="https://go.creative-biolabs.com/sdab-development-for-hapten-target.html">sdAb Development for Hapten Target</a> </div> <div class="col-md-4"> <div class="pagerow_pt_unit_2"> <img loading="lazy" src="/resource/sdab/images/Case-Study-1.jpg" width="530" height="500" alt="sdAb Development for 3 Targets via 1 Camelid Immunization" /> </div> <a href="https://go.creative-biolabs.com/sdab-development-for-3-targets-via-1-camelid-immunization.html">sdAb Development for 3 Targets via 1 Camelid Immunization</a> </div> <div class="col-md-4"> <div class="pagerow_pt_unit_2"> <img loading="lazy" src="/resource/sdab/images/Case-Study-2.jpg" width="530" height="500" alt="sdAb Development via Premade HuSdL-1 Library" /> </div> <a href="https://go.creative-biolabs.com/sdab-development-via-premade-husdl-1-library.html">sdAb Development via Premade HuSdL-1 Library</a> </div> <div class="col-md-4"> <div class="pagerow_pt_unit_2"> <img loading="lazy" src="/resource/sdab/images/Case-Study-3.jpg" width="530" height="500" alt="sdAb Development for Low Immunogenic Target" /> </div> <a href="https://go.creative-biolabs.com/sdab-development-for-low-immunogenic-target.html">sdAb Development for Low Immunogenic Target</a> </div> </div> </div>  <div class="tab_cont"> <div class="row pagerow_pt"> <div class="col-md-4"> <div class="pagerow_pt_unit"> <img loading="lazy" src="../static/img/sdAb-Discovery-and-Engineering-pic.png" width="600" height="500" alt="Anti-idiotypic Antibody Developmen" /> </div> <a href="/resource/sdab/pdf/com/brochure/Single-Domain-Antibody-Discovery-Creative-Biolabs-2019.pdf">sdAb Discovery and Engineering</a> </div> </div> </div> </div> <script> window.addEventListener('load',function(){ // $(function () { Tab_("tab-box-con"); // }) }); </script><br> <p class="pronote"><i class="fa fa-exclamation-triangle"></i>All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.</p> <div class="related-sections"> <b>Related Sections</b> <b>Services</b> <ul class="related-sections-ul"> <li><a href="/phage-display-library-construction.html" >Phage Display Library Construction Services</a></li> <li><a href="/Human-Single-Domain-Antibody-Production-using-Transgenic-Mice.html" >Transgenic Mice based Magic™ Human Single Domain Antibody Discovery Service</a></li> <li><a href="/phage-display-library-screening.html" >Phage Display Library Screening Services</a></li> <li><a href="/Native-TM-Camel-Antibody-Discovery-Service.html" >Single B Cell Sorting based Native™ Camel Antibody Discovery Service</a></li> <li><a href="/Native-TM-Shark-Antibody-Discovery-Service.html" >Single B Cell Sorting based Native™ Shark Antibody Discovery Service</a></li> </ul> <b>Products</b> <ul class="related-sections-ul"> <li><a href="http://www.creativebiolabs.net/single-domain-antibodies_11.htm" target="_blank">Single Domain 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