Antibody Conversion Services - Creative Biolabs

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With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects. </p>  <b class="Highlights_tit">Highlights</b> <ul class="HighlightsArea"> <li class="col-md-6 col-lg-6 col-sm-6"> <img src="static/images/Solutions-menu-lights-pic-1.jpg" srcset="static/images/Solutions-menu-lights-pic-1.webp 1x" width="201" height="131" loading="lazy"><a href="discovery-services.html" class="bannerbutton"><i class="fa fa-angle-right"></i>Discovery Services</a> </li> <li class="col-md-6 col-lg-6 col-sm-6"> <img src="static/images/Solutions-menu-lights-pic-2.jpg" srcset="static/images/Solutions-menu-lights-pic-2.webp 1x" width="201" height="131" loading="lazy"><a href="engineering-services.html" class="bannerbutton"> <i class="fa fa-angle-right"></i>Engineering Services </a> </li> </ul> </li> </ul> </div> </div> </li> <li> <span>Characterization</span> <div class="secondMenu"> <div class="second-menu-area-box"> <ul style="margin-top:0;">  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You are also welcome to contact us directly for more specific solutions. </p>  <b class="Highlights_tit">Highlights</b> <ul class="HighlightsArea"> <li class="col-md-6 col-lg-6 col-sm-6"> <img src="static/images/Resources-menu-lights-pic-1.jpg" srcset="static/images/Resources-menu-lights-pic-1.webp 1x" width="201" height="131" loading="lazy"><a href="Peer-reviewed-Publications.html" class="bannerbutton"><i class="fa fa-angle-right"></i>Peer-Reviewed Publications</a> </li> <li class="col-md-6 col-lg-6 col-sm-6"> <img src="static/images/Resources-menu-lights-pic-2.jpg" srcset="static/images/Resources-menu-lights-pic-2.webp 1x" width="201" height="131" loading="lazy"><a href="support-documents.html" class="bannerbutton"><i class="fa fa-angle-right"></i>Support Documents</a> </li> </ul> </li> </ul> </div> </div> </li> <li> <span>Company</span> <div class="secondMenu"> <div class="second-menu-area-box"> <ul style="margin-top:0;">  <li class="col-md-8 col-lg-8 col-sm-8"> <ul class=" MenuList"> <li class="col-md-4 col-lg-4 col-sm-4"><a href="aboutus.html" rel="nofollow">About Creative Biolabs</a> <ul class="navi-menu-2 three_menu"> <li class="navi-menu-li-2"><a href="/Licensing-and-Collaboration.html" title="Licensing and Collaboration">Licensing and Collaboration</a></li> </ul> </li> <li class="col-md-4 col-lg-4 col-sm-4"><a href="contactus.html" rel="nofollow">Contact Us</a></li> <li class="col-md-4 col-lg-4 col-sm-4"><a href="Distributors.html" rel="nofollow">Distributors</a></li> <li class="col-md-4 col-lg-4 col-sm-4"><a href="feedback.aspx">Reviews & Endorsements</a></li> <li class="col-md-4 col-lg-4 col-sm-4"><a href="/career/career.aspx" rel="nofollow">Careers</a> </li> <li class="col-md-4 col-lg-4 col-sm-4"><a href="https://events.creative-biolabs.com" rel="nofollow">Events</a></li> <li class="col-md-4 col-lg-4 col-sm-4"><a href="scholarship-program.html">Scholarship Program</a></li> <li class="col-md-4 col-lg-4 col-sm-4"><a href="creative-biolabs-corporate-social-responsibility.html">Corporate Social Responsibility</a></li> </ul> </li> <li class="col-md-4 col-lg-4 col-sm-4"> <b class="Highlights_tit"> About Us </b> <p class="open-sans-light"> Get a real taste of Creative Biolabs, one of the most professional custom service providers in the world. We are committed to providing highly customized comprehensive solutions with the best quality to advance your projects. </p> <b class="Highlights_tit">Highlights</b> <ul class="HighlightsArea"> <li class="col-md-6 col-lg-6 col-sm-6"> <img src="static/images/Abouts-menu-lights-pic-1.jpg" srcset="static/images/Abouts-menu-lights-pic-1.webp 1x" width="200" height="131" loading="lazy"><a href="aboutus.html" class="bannerbutton"> <i class="fa fa-angle-right"></i>About CBL </a> </li> <li class="col-md-6 col-lg-6 col-sm-6"> <img src="static/images/Abouts-menu-lights-pic-2.jpg" srcset="static/images/Abouts-menu-lights-pic-2.webp 1x" width="200" height="131" loading="lazy"><a href="contactus.html" class="bannerbutton"> <i class="fa fa-angle-right"></i>Contact Us </a> </li> </ul> </li> </ul> </div> </div> </li> </ul> <script> window.addEventListener('load',function(){ var arr = ['/drug-discovery/therapeutics/','/bioconjugation/','/adc/']; $('.mennav ul li a').each(function(){ var $this = $(this); var href = $this.attr("href").toLocaleLowerCase(); $.each(arr, function(index, value) { if (href && href.startsWith(value)) { $this.attr('target', '_blank'); } }); }); }); </script> </div> <div class="top-search search-group"> <div class="col-md-5 col-sm-6"> <label class="radio-inline"><input type="radio" name="search" class="rdo_productsearch" value="product" data-type="product" placeholder="Please input cat#, product name, abbr, or keywords."> Product</label> <label class="radio-inline"><input type="radio" name="search" class="rdo_servicesearch" value="service" data-type="service" placeholder="Please input your keywords."> Service</label> <label class="radio-inline"><input type="radio" name="search" class="rdo_googlesearch" value="google" data-type="google" placeholder="Please input your keywords." checked="checked"> Keyword</label> </div> <div class="col-md-7 col-sm-6 search-div"> <input id="keys" name="keys" placeholder="Please input your keywords." class="search-input"> <div class="selectItems"></div> <span class="search_btn"><i class="fa fa-search"></i></span> </div> </div> </div> <div class="searchShow"> <div class="searchtopbg"></div> <span class="searchclosed"><i class="fa fa-times"></i>Close</span> <div class="addsearch search-group"></div> </div> <div class="banner_inpage services_bg_2"> <div class="auto-container small_content-width about_container"> <ul class="bread-crumb clearfix"> <li><a href="/">Home</a></li> <li><a href="javascrpt:void(0)">Engineering</a></li> <li>Antibody Conversion</li> </ul> <p>"Creative Biolabs is committed to providing highly customized comprehensive solutions with the best quality to advance our global clients’ projects."</p> </div> </div> <script> window.addEventListener('load', function () { if (($('.bread-crumb li:last-child').text()) == "Peer-Reviewed Publications") { $(".banner_inpage").addClass("peer-reviewed-publications_bg").removeClass("services_bg_2"); } if ($(window).hasClass('bread-crumb')) { if ($(".bread-crumb").html().indexOf("RESOURCE") > -1) { $(".pronote").hide(); } if ($(".bread-crumb").html().indexOf("Careers") > -1) { $(".pronote").hide(); } } }); </script> <div class="small_content-width services-container"> <div class="col-md-9"> <div class="inpage_section_title"> <h1>Antibody Conversion Services</h1> </div> <div class="page-tab"> <span onclick="window.location.href=window.location.pathname+'#tab1'">Background</span> <span onclick="window.location.href=window.location.pathname+'#tab2'">Services</span> <span onclick="window.location.href=window.location.pathname+'#tab3'">Published Data</span> <span onclick="window.location.href=window.location.pathname+'#tab4'">FAQ</span> <span onclick="window.location.href=window.location.pathname+'#tab5'">Resources</span> </div> <script> window.addEventListener('load',function(){ page_tab_margin() page_tab_css(); })</script> <p> <b>Creative Biolabs</b> is a leading service provider that focuses on antibody engineering for research, diagnostic and therapeutic use. Our service portfolio includes <a href="/antibody-affinity-maturation.html">antibody affinity maturation</a>, <a href="/Antibody-Stability-Improvement.html">antibody stability improvement</a>, <a href="/Chimeric-Antigen-Receptor-CAR-construction-Service.html">chimeric antigen receptor (CAR) construction</a>, <a href="/scfv-fab-generation.html">scFv/Fab construction</a> and other antibody specialization and modification services. </p> <h2 class="h2_tit page_tab_margin" id="tab1"> Background </h2> <p> Since the first hybridoma has been obtained, a kind of possibility has arisen to modify the antibody to enhance its functionality. During this decade, antibody has become a major class of new drugs to treat several disease indications including cancer and autoimmune diseases. As the foundation of many diagnostics and therapeutics, the specific antigen binding ability of antibodies is critical for variety purposes, such as expanding detection limits, extending dissociation half-life time, decreasing therapeutic dosages and increasing overall efficacy. Nevertheless, it is still beneficial to optimize these antibodies or antibody related molecules with respect to their activity. </p> <p> Through modern molecular biology techniques, interested antibodies can be specific modified or engineered to suit the application. If the heavy- and light-chain sequences are determined, the interested antibody can be reconfigured into different isotypes or a variety of formats (scFv, Fab, sdAb, bispecific antibody, etc.) and other possible modification including affinity measurement, humanization, caninization and so on. One feasible way is done by sequencing and cloning the interested region of antibody and grafting this select region onto a suitable framework to construct a plasmid. The predicted antibody can then be produced by transient transfection using appropriate expression system, such as mammalian cells and <i>E. coli</i> strains. As a choice, antibody modification can also incorporate with phage or other display methods to obtain a much broader candidates or select the most ideal products for numerous applications. Notably, all these modification and manufacture can be perfectly implemented by <b>Creative Biolabs</b> who is a well-recognized expert in the field of antibody development and phage display technology. </p> <h2 class="h2_tit page_tab_margin" id="tab2"> Antibody Engineering Services </h2> <p> <b>Creative Biolabs</b> has devoted to the field of antibody engineering for over decade and established a series of comprehensive platforms to integrate various antibody engineering services. With the extensive experience, abundant industry knowledge and advanced technical skills, our scientists are fully confident in accomplishing even the most challenging antibody engineering project. </p> <p class="ServiceShowPic"> <img loading="lazy" width="320" height="314" alt="" src="static/img/2-Antibody Engineering Services.png" /> </p> <p> Learn more about <a href="/antibody-engineering-services.html">antibody engineering services</a>: </p> <ul class="ullist"> <li> <a href="/antibody-affinity-maturation.html">Antibody Affinity Maturation</a> </li> <li> <a href="/Antibody-Stability-Improvement.html">Antibody Stability Improvement</a> </li> <li> <a href="/Simianization-of-Non-monkey-Antibodies.html">Simianization of Non-monkey Antibodies</a> </li> <li> <a href="/Antibody-Caninization-Service.html">Monoclonal Antibody Caninization Service</a> </li> <li> <a href="/Antibody-Murinization-Service.html">Monoclonal Antibody Murinization Service</a> </li> <li> <a href="/Custom-Cysteine-Modification-in-Antibody.html">Custom Cysteine Modification in Antibody</a> </li> </ul> <h2 class="h2_tit page_tab_margin" id="tab3"> Published Data </h2> <ul class="ullist"> <li> <strong>The Engineering of Human IgG3 Antibody to Reduce Aggregation</strong> </li> </ul> <p class="show-center"> <img loading="lazy" src="/static/img/1-2-4-antibody-engineering-services-2.jpg" width="400" height="220" alt="" />Fig. 2 Amino acid alignment of the heavy chain CH3 domain of human IgG1 and IgG3 and summary of the amino acid substitution antibodies constructed in this study. (Seiji Saito, 2019) </p> <p> The human IgG3 subclass has the characteristics of high effect function and Fab arm flexibility. Here, the researchers used anti-CD20 IgG3 as a model to study the formation of IgG3 aggregates and the potential of such antibodies in treatment. The researchers swapped the constant domain of IgG3 with the constant domain of stable IgG1 to avoid a large number of aggregations of anti-CD20 IgG3 antibodies. The results showed that the collective formation of IgG3 antibodies with the IgG1 CH3 domain decreased. It was found that two amino acid mutations in the CH3 domain reduced the aggregation of IgG3 and increased the CH3 transition temperature. They improved the engineered human IgG3 antibody by further mutation and obtained IgG3KVH to achieve protein A binding and showed antigenic binding similar to that of wild-type IgG3. At the same time, IgG3KVH also has high binding activity to Fcγ RIIIa and C1q. In this study, the researchers obtained an engineered human IgG3 antibody with low aggregation, which will help to research and develop therapeutic antibodies with high effect function and Fab arm flexibility. </p> <div class="reference"> <strong>Reference</strong> <ol class="ollist decimal"> <li> Saito, Seiji, et al. "A stable engineered human IgG3 antibody with decreased aggregation during antibody expression and low pH stress." <em>Protein Science</em> 28.5 (2019): 900-909. </li> </ol> </div> <script type="text/javascript"> window.addEventListener('load',function(){ faqlist(4); }); </script> <h2 class="h2_tit page_tab_margin" id="tab4"> FAQ </h2> <ol class="ollist faqlist"> <li> <span class="sFAQTit">What is antibody engineering?</span> <p> Antibody engineering is a branch of biotechnology that involves the modification and synthesis of antibodies to enhance their effectiveness for specific applications. This can include altering the antibody's binding affinity, specificity, stability, or other pharmacological properties to make it more suitable for therapeutic use, diagnostic tools, or research purposes. </p> </li> <li> <span class="sFAQTit">How are antibodies engineered?</span> <p> There are several methods used to engineer antibodies, including phage display, where bacteriophages are used to evolve new antibodies; genetic engineering, where the DNA encoding antibodies is modified; and hybridoma technology, which involves fusing B-cells with immortal cells to produce monoclonal antibodies. Recent advancements also include the use of computational design and machine learning to predict and improve antibody structures. </p> </li> <li> <span class="sFAQTit">What are the applications of engineered antibodies?</span> <p> Engineered antibodies have a wide range of applications in medicine and research. In therapeutics, they are used as the basis for many antibody-drug conjugates, cancer immunotherapies, and treatments for autoimmune diseases. In diagnostics, engineered antibodies provide high specificity in tests for various diseases, including infectious diseases and cancer biomarkers. </p> </li> <li> <span class="sFAQTit">How do antibody fragments fit into antibody engineering?</span> <p> Antibody fragments such as Fab, F(ab')2, and single-chain variable fragments (scFv) play a crucial role in antibody engineering. These fragments retain the antigen-binding part of the antibody but lack the Fc region, which can be advantageous for reducing immunogenicity and improving tissue penetration. Engineering antibody fragments is particularly useful in therapeutic contexts where full-length antibodies might be too large or where non-essential parts of the antibody might trigger unwanted immune responses. </p> </li> <li> <span class="sFAQTit">What role does computational biology play in antibody engineering?</span> <p> Computational biology has become pivotal in antibody engineering, especially in predicting antibody structure, improving binding affinity, and identifying potential immunogenicity. Techniques such as molecular modeling, simulation, and machine learning algorithms can predict how mutations affect antibody behavior, guide the design of antibody libraries, and optimize antibodies for specific applications without the need for extensive in vitro testing. </p> </li> <li> <span class="sFAQTit">Can engineered antibodies be used in all types of diseases?</span> <p> Engineered antibodies are most prominently used in the treatment of cancers and autoimmune diseases due to their ability to be tailored to target specific pathological antigens. However, their use is expanding to infectious diseases, neurological disorders, and other conditions. The versatility of engineered antibodies lies in their ability to be customized for different targets and mechanisms, although their effectiveness can vary based on the nature of the disease and the target antigen's characteristics. </p> </li> </ol> <h2 class="h2_tit page_tab_margin" id="tab5"> Resources </h2> <p> Use the resources in our library to help you understand your options and make critical decisions for your study. </p> <div class="tab_nav"> <span class="tab_choosebg">Videos</span><span>Podcasts</span><span>Infographic</span><span>Flyer</span><span>Case study</span><span>Articles</span> </div>  <div class="tab_cont" style="display:block;"> <div class="row pagerow_pt"> <div class="col-md-4"> <div class="pagerow_pt_unit"> <img loading="lazy" src="../static/img/introduction-of-recombinant-antibody.jpg" alt="" /> </div> <a href="https://youtu.be/0-yZ9dTGg18" target="_blank">Introduction of Recombinant Antibody</a> </div> <div class="col-md-4"> <div class="pagerow_pt_unit"> <img loading="lazy" src="../static/img/development-of-monoclonal-antibodies.jpg" alt="" /> </div> <a href="https://youtu.be/O5_abD0eiIE" target="_blank">Development of Monoclonal Antibodies</a> </div> <div class="col-md-4"> <div class="pagerow_pt_unit"> <img loading="lazy" src="../static/img/video-phage-display-technology.png" alt="Phage Display Technology" /> </div> <a href="https://youtu.be/mczJ3ZbpVW8" target="_blank">Phage Display Technology</a> </div> <div class="col-md-4"> <div class="pagerow_pt_unit"> <img loading="lazy" src="../static/img/antibody-humanization-service.jpg" alt="Antibody Humanization Service" /> </div> <a href="https://youtu.be/cOYkgrybIJk" target="_blank">Antibody Humanization Service</a> </div> <div class="col-md-4"> <div class="pagerow_pt_unit"> <img loading="lazy" src="../static/img/video-de-novo-antibody-sequencing-service.jpg" alt="" /> </div> <a href="https://youtu.be/lcdGTNKbaGQ" target="_blank"><em>De Novo</em> Antibody Sequencing Service</a> </div> </div> </div>  <div class="tab_cont"> <div class="therapy_ad"> <a href="therapeutic-antibody.html"> <img loading="lazy" src="../static/img/podcasts-ad.png" width="1700" height="500" srcset="../static/img/podcasts-ad.webp 1x,../static/img/podcasts-ad.webp 2x,../static/img/podcasts-ad.webp 3x" alt="In this brand new podcast series, we will show you everything you want to know about CAR-T therapy, including the mechanism, current applications, technology limitations, and potential strategies." /></a> </div> </div>  <div class="tab_cont"> <div class="row pagerow_pt"> <div class="col-md-4"> <div class="pagerow_pt_unit_2"> <img loading="lazy" src="/resource/images/A-Brief-Introduction-to-Antibodies-1.jpg" width="600" height="500" alt="" /> </div> <a href="https://go.creative-biolabs.com/a-brief-introduction-to-antibodies.html">A Brief Introduction to Antibodies</a> </div> <div class="col-md-4"> <div class="pagerow_pt_unit_2"> <img loading="lazy" 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