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Primer Walking | GENEWIZ from Azenta
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bold;">NEW</sup></a></li></ul></div><div class='col-md-3 '><ul class='list-unstyled'><li><a class='GColor' href='/en/Public/Services/Sanger-Sequencing' style='color:#826ec3;font-size:17px'>Sanger Sequencing</a></li><li><a href='/en/Public/Services/Sanger-Sequencing/Sanger-EZ' style='color:#585F69;font-size:14px'>Sanger-EZ <sup style="color:#826ec3; font-weight: bold;">NEW</sup></a></li><li><a href='/en/Public/Services/Sanger-Sequencing/AAV-ITR' style='color:#585F69;font-size:14px'>AAV-ITR Sanger Sequencing</a></li><li><a href='/en/Public/Services/Sanger-Sequencing/Purified-Templates' style='color:#585F69;font-size:14px'>Purified Templates</a></li><li><a href='/en/Public/Services/Sanger-Sequencing/Difficult-Template-Sequencing' style='color:#585F69;font-size:14px'>Difficult Template Sequencing</a></li><li><a href='/en/Public/Services/Sanger-Sequencing/Direct-Colony-Sequencing' style='color:#585F69;font-size:14px'>Direct Colony Sequencing</a></li><li><a href='/en/Public/Services/Sanger-Sequencing/PCR-Purification' style='color:#585F69;font-size:14px'>PCR Purification</a></li><li><a href='/en/Public/Services/Sanger-Sequencing/Primer-Walking' style='color:#585F69;font-size:14px'>Primer Walking</a></li><br><li><a class='GColor' href='/en/Public/Services/Molecular-Genetics' style='color:#826ec3;font-size:17px'>PCR + Sanger Services</a></li><li><a href='/en/Public/Services/Molecular-Genetics/Bacterial-and-Fungal-Identification' style='color:#585F69;font-size:14px'>Bacterial and Fungal Identification</a></li><li><a href='/en/Public/Services/Molecular-Genetics/Mutation-Analysis' style='color:#585F69;font-size:14px'>Mutation Analysis</a></li><li><a href='/en/Public/Services/Molecular-Genetics/SNP-Genotyping' style='color:#585F69;font-size:14px'>SNP Genotyping</a></li><li><a href='/en/Public/Services/Molecular-Genetics/PCR-Plus-Sequencing-CRISPR-Analysis-Package' style='color:#585F69;font-size:14px'>PCR Plus Sequencing CRISPR Analysis Package</a></li><li><a href='/en/Public/Services/Molecular-Genetics/cDNA-Verification' style='color:#585F69;font-size:14px'>Confirmatory Sequencing & Cell Bank Characterization</a></li><li><a href='/en/Public/Services/Molecular-Genetics/Yeast-Colony-Sequencing' style='color:#585F69;font-size:14px'>Yeast Colony Sequencing</a></li><li><a href='/en/Public/Services/Molecular-Genetics/Fragment-Analysis' style='color:#585F69;font-size:14px'>Fragment Analysis</a></li><li><a href='/en/Public/Services/Molecular-Genetics/PCR-Solutions' style='color:#585F69;font-size:14px'>Quantitative PCR & Digital PCR <sup style="color:#826ec3;"><strong>NEW </strong></sup></a></li><li><a href='/en/Public/Services/Molecular-Genetics/Nucleic-Acid-Extraction' style='color:#585F69;font-size:14px'>Nucleic Acid Extraction</a></li></ul></div><div class='col-md-3 '><ul class='list-unstyled'><li><a class='GColor' href='/en/Public/Services/Gene-Synthesis' style='color:#826ec3;font-size:17px'>DNA & RNA Synthesis</a></li><li><a href='/en/Public/Services/Gene-Synthesis/Gene-Synthesis' style='color:#585F69;font-size:14px'>Gene Synthesis</a></li><li><a href='/en/Public/Services/Gene-Synthesis/Antibody-DNA-Synthesis' style='color:#585F69;font-size:14px'>Antibody Synthesis</a></li><li><a href='/en/Public/Services/Gene-Synthesis/AAV-Plasmid-Synthesis' style='color:#585F69;font-size:14px'>AAV Plasmid Synthesis</a></li><li><a href='/en/Public/Services/Gene-Synthesis/Gene-Fragments' style='color:#585F69;font-size:14px'>Gene Fragments</a></li><li><a href='/en/Public/Services/Gene-Synthesis/CRISPR-Construct-Synthesis' style='color:#585F69;font-size:14px'>CRISPR Construct Synthesis</a></li><li><a href='/en/Public/Services/Gene-Synthesis/Synthetic-DNA-Libraries' style='color:#585F69;font-size:14px'>Synthetic DNA Libraries</a></li><li><a href='/en/Public/Services/Gene-Synthesis/Single-Stranded-DNA-Synthesis' style='color:#585F69;font-size:14px'>ssDNA Synthesis</a></li><li><a href='/en/Public/Services/Gene-Synthesis/DNA-Cloning' style='color:#585F69;font-size:14px'>DNA Cloning</a></li><li><a href='/en/Public/Services/Gene-Synthesis/Site-Directed-Mutagenesis' style='color:#585F69;font-size:14px'>Site-Directed Mutagenesis<br></a></li><li><a href='/en/Public/Services/Gene-Synthesis/Viral-Package' style='color:#585F69;font-size:14px'>Viral Packaging <sup style="color:#826ec3;"><strong>NEW </strong></sup></a></li><li><a href='/en/Public/Services/Gene-Synthesis/Recombinant-Antibody-Production' style='color:#585F69;font-size:14px'>Recombinant Antibody Production <sup style="color:#826ec3;"><strong>NEW </strong></sup></a></li><li><a href='/en/Public/Services/Gene-Synthesis/Custom-mRNA-Synthesis' style='color:#585F69;font-size:14px'>Custom mRNA Synthesis <sup style="color:#826ec3; font-weight: bold;">NEW</sup></a></li><br><li><a class='GColor' href='/en/Public/Services/Oligo' style='color:#826ec3;font-size:17px'>Oligo Synthesis Services</a></li><li><a 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Plasmid Preparation</a></li><br><li><a class='GColor' href='/en/Public/Services/Clinical-Services' style='color:#826ec3;font-size:17px'>PreClinical and Clinical Services</a></li><li><a href='/en/Public/Services/Clinical-Services/Clinical-Services-Overview' style='color:#585F69;font-size:14px'>Clinical Services</a></li><li><a href='/en/Public/Services/Clinical-Services/Microarray-Solutions' style='color:#585F69;font-size:14px'>Microarray Solutions <sup style="color:#826ec3; font-weight: bold;">NEW</sup></a></li><li><a href='/en/Public/Services/Clinical-Services/CLIA-Variant-Confirmation' style='color:#585F69;font-size:14px'>CLIA Variant Confirmation (PCR + Sanger)</a></li><li><a href='/en/Public/Services/Clinical-Services/CLIA-Sanger-Sequencing' style='color:#585F69;font-size:14px'>CLIA Sanger Sequencing</a></li><li><a href='/en/Public/Services/Clinical-Services/CLIA-Whole-Exome-Sequencing' style='color:#585F69;font-size:14px'>CLIA Whole Exome Sequencing</a></li><li><a href='/en/Public/Services/Clinical-Services/CLIA-Whole-Genome-Sequencing' style='color:#585F69;font-size:14px'>CLIA Whole Genome Sequencing</a></li><li><a href='/en/Public/Services/Clinical-Services/Regulatory' style='color:#585F69;font-size:14px'>GLP-Compliant Overview</a></li><li><a href='/en/Public/Services/Clinical-Services/GLP-compliant-Confirmatory-Sequencing' style='color:#585F69;font-size:14px'>GLP Confirmatory Sequencing</a></li><li><a href='/en/Public/Services/Clinical-Services/GLP-compliant-SNP-Mutation-Analysis' style='color:#585F69;font-size:14px'>GLP SNP Mutation Analysis</a></li><li><a href='/en/Public/Services/Clinical-Services/GLP-compliant-Plasmid-Prep' style='color:#585F69;font-size:14px'>GLP Plasmid Prep</a></li><li><a href='/en/Public/Services/Clinical-Services/Nucleic-Acid-Extraction' style='color:#585F69;font-size:14px'>GLP Nucleic Acid Extraction</a></li><li><a href='/en/Public/Services/Clinical-Services/Biofluid-Processing-Overview' style='color:#585F69;font-size:14px'>Biofluid Processing</a></li><li><a href='/en/Public/Services/Clinical-Services/CLIA-Integration-Site-Analysis' style='color:#585F69;font-size:14px'>CLIA Integration Site Analysis <sup style="color:#826ec3; font-weight: bold;">NEW</sup></a></li></ul></div></div></div></li></ul></li><li class='dropdown'><a href='/en/Public/Research-Areas' data-toggle='dropdown' class='dropdown-toggle' style='color:white;height:52px;top:47px;padding-top:0;background-color:transparent;font-size:17px;font-weight:bold;'>RESEARCH AREAS</a><ul class='dropdown-menu'><li class='dropdown'><a class='GColor' href='/en/Public/Research-Areas/AAV' style='font-size:17px'>AAV Services</a><ul class='dropdown-menu'></ul></li><li class='dropdown'><a class='GColor' href='/en/Public/Research-Areas/Agriculture-Food-Science' style='font-size:17px'>Agriculture and Food Science</a><ul class='dropdown-menu'></ul></li><li class='dropdown'><a class='GColor' 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padding: 0; } .walking-new-container { background: #f0f0f0; padding: 30px 0; } .walking-new-container h1 { margin: 0; color: #000000; } .walking-new-content p { color: #000000; } .walking-new-content { width: 1170px; margin: 0 auto; } .walking-content-box { display: inline-block; vertical-align: middle; /*width: 50%;*/ } #walking-content-box-left { width: 60%; text-align: left; padding: 30px 0; } #walking-content-box-right { width: 39%; text-align: right; } .walking-content-box img { width: 400px; } .walking-content-box button { border: none; border-radius: 4px; background: #826ec3; padding: 10px 20px; color: #fff; font-size: 15px; letter-spacing: 1px; } .walking-content-box button:hover { background: #6965a1; } @media only screen and (max-width: 768px) { .headline-center { padding-top: 0; } .walking-new-container { text-align: center; margin-top: 0; } .walking-new-content { width: 90%; margin: 0 auto; } .walking-new-content { display: inline-block; } #walking-content-box-right, #walking-content-box-left { width: 100%; text-align: center; } #walking-content-box-right { margin-top: 20px; } } @media only screen and (max-width: 1170px) { .walking-new-content { width: 90%; margin: 0 auto; } .walking-content-box img { width: 350px; } } </style> <div class="walking-new-container"> <div class="walking-new-content"> <div class="walking-content-box" id="walking-content-box-left"> <h1>Primer Walking</h1> <div> </div> <p>Primer Walking utilizes multiple primers in a series to sequence entire plasmids or PCR products. GENEWIZ Primer Walking service options include <a href="#Confirmation">plasmid sequence confirmation</a> for already known sequences and <a href="#Discovery">plasmid sequence discovery</a> for unknown sequences. For AAV vectors, we offer primer walking plus our new, proprietary <a href="#AAV">AAV-ITR Sequencing</a>. Trust GENEWIZ from Azenta to manage your project from primer design to variance analysis and report generation.<a id="Confirmation"></a></p> <p> </p> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://clims4.genewiz.com/PrimerWalking?lobId=1&serviceId=109" target="_blank"><button>Place Order</button></a> </div> <div class="walking-content-box" id="walking-content-box-right"><a href="https://web.genewiz.com/primer-walking-25-0" target="_blank"> <img alt="" src="/-/media/Images/Primer_Walking_plus_AAV_Banner_Right_Ad.ashx?la=en&hash=66511E2311786A1D33F83389ACABAFF76656B387" /></a> </div> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> .conf-container { text-align: center; background: #fff; width: 100%; } .conf-service { width: 1170px; display: inline-block; vertical-align: middle; padding-top: 10px; } .conf-service h2 { text-align: center; } .conf-service p { text-align: center; padding: 10px; } .conf-work { width: 19%; height: auto; display: inline-block; text-align: center; padding-bottom: 20px; } @media only screen and (max-width: 900px){ .conf-container { width: 100%; } .conf-service { width: 100%; } .conf-work { width: 90%; } } </style> <div class="conf-container"> <div class="conf-service"> <h2>PLASMID SEQUENCE CONFIRMATION service</h2> <p><a id="Confirmation"></a>Azenta’s primer walking sequence confirmation service offers peace of mind and helps you perform plasmid identity verification thus avoiding costly complications with downstream applications. Estimated turnaround time is 3-5 business days after primer design. We also offer <a href="https://www.genewiz.com/en/Public/Services/Clinical-Services/GLP-compliant-Confirmatory-Sequencing">GLP level plasmid confirmatory sequencing.</a></p> <p><strong>With our plasmid confirmation service you can: </strong><br> <br> • Confirm the sequence of your cloned products/genes of interest<br> • Sequence your entire plasmid, including difficult-to-amplify regions<br> • Examine failed amplifications and incorrect restriction digest patterns<br> •<strong> Sequence full length AAV plasmids and hard to sequence ITR regions </strong></p> <p><a id="Discovery"></a> </p> <div class="conf-work"> <img alt="" src="/-/media/Images/Submit_Samples.ashx?la=en&hash=8458FC0AC5AD739AB43731E71BADF2C71E0C700A"> <h3>1. Submit Samples<br> <br> </h3> </div> <div class="conf-work"> <img alt="" src="/-/media/Images/Primer_Design.ashx?la=en&hash=CA35FD1F6A6BB09661FBA276FECBA2A473FCFE4A"> <h3>2. Primer Design*<br> <br> </h3> </div> <div class="conf-work"> <img alt="" src="/-/media/Images/Sanger_Sequencing.ashx?la=en&hash=2430F267F873D28CD006A0E2B426427A9182A34A"> <h3>3. Sanger Sequencing<br> <br> </h3> </div> <div class="conf-work"> <img alt="" src="/-/media/Images/Consensus_Sequence_Assembly.ashx?la=en&hash=A62C6EE64BB410C5E6569631111F9E5770819F00"> <h3>4. Consensus Sequence Assembly</h3> </div> <div class="conf-work"> <img alt="" src="/-/media/Images/Data_Report.ashx?la=en&hash=8593977624D07530CBE5026115F7BB1395B6F4AC"> <h3>5. Data Report<br> <br> </h3> </div> <a id="Discovery"></a> <p>*Please note, a reference sequence is required for primer design.</p> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> .disc-container { text-align: center; display: inline-block; vertical-align: middle; background: #f1f0f0; width: 100%; } .disc-service { width: 1170px; padding: 20px 0 20px 0; display: inline-block; vertical-align: middle; } .disc-service h2 { text-align: center; } .disc-service p { text-align: center; padding: 0 10 0 10; } .disc-work { width: 19%; height: auto; display: inline-block; text-align: center; padding-bottom: 20px; } .disc-work-1 { width: 38%; height: auto; display: inline-block; text-align: center; padding-bottom: 20px; } @media only screen and (max-width: 900px){ .disc-container { width: 100%; } .disc-service { width: 100%; } .disc-work { width: 90%; } .disc-work-1 { width: 90%; } } </style> <div class="disc-container"> <div class="disc-service"> <h2>PLASMID SEQUENCE DISCOVERY service</h2> <p>Azenta’s primer walking sequence discovery service maps your unknown plasmid or PCR product for use in downstream applications. Subsequent primers will be designed as sequences are generated. Estimated turnaround time is 3-5 days per 1kb.</p> <p> </p> <p><strong>With our plasmid discovery service you can: </strong><br> <br> • Create a plasmid map for your undetermined plasmids <br> • Confirm the sequence of your mystery constructs<br> • Identify unknown regions of your plasmids<br> • <strong>Sequence full length AAV plasmids and hard to sequence ITR regions</strong> </p> <p><a id="AAV"></a></p> <div class="disc-work"> <img alt="" src="/-/media/Images/Submit_Samples_Text.ashx?la=en&hash=AB88ABDDB02691816C77A2F3F0937D34608FECF2"> </div> <div class="disc-work-1"> <img alt="" src="/-/media/Images/Primer_Design_Sanger_Seq_Text.ashx?la=en&hash=6E0907B51593D7E3E6DD9BC00E68F70FBC2A5F29"> </div> <div class="disc-work"> <img alt="" src="/-/media/Images/Consensus_Seq_Assembly_Text.ashx?la=en&hash=2616EF08E991D2F92271D243134F0C1D92FC1F55"> </div> <div class="disc-work"> <img alt="" src="/-/media/Images/Data_Report_Text.ashx?la=en&hash=EDBF3E89813B8D17953E93E8DB5BB45FD00D8A8D"> </div> <a id="AAV"></a> <p>*As a starting point, an antibiotic resistance gene, a known promoter (M13, T7), or another known sequence will be required for the initial primer design. </p> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <link href="https://fonts.googleapis.com/css?family=Roboto:300,400,500,700" rel="stylesheet" /> <style type="text/css"> .covid-banner { width: 1170px; height: auto; margin: 0 auto; background-image: url("/-/media/11038-D_PEZ-DirectColonySeq_WebBanFull-Med_Background.ashx"); background-size: cover; background-position: center; text-align: left; padding: 0px 0 0px 0; } .covid-content { text-align: center; } .covid-box h3 { color: #ffffff; text-transform: none; letter-spacing: 1px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; line-height: 115%; font-size: 40px; margin: 0; padding-bottom: 10px; padding-top: 15px; } .covid-box h4 { color: #ffffff; text-transform: none; letter-spacing: 1px; line-height: 30px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 35px; margin: 0; margin-bottom: 5px; } .covid-box p { color: #000000; text-transform: none; letter-spacing: 1px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 15px; margin: 0; margin-bottom: 5px; } .covid-box button { padding: 8px 19px; color: #826ec3; letter-spacing: 1px; background: #ffffff; border: 2px solid #ffffff; border-radius: 0px; transition: all .2s ease-in-out; font-size: 15px } .covid-box button:hover { cursor: pointer; transform: scale(1.1); } .covid-box button:focus { outline: 0; } @media only screen and (max-width: 1170px) { .covid-banner { width: 100%; height: auto; } .covid-content { width: 90%; margin: 0 auto; } } </style> <br /> <div class="covid-banner"> <div class="covid-content"> <div class="covid-box"> <br /> <h3><span style="color: #55c8d2;">NEW</span> Direct Colony to<br /> Whole Plasmid Sequencing</h3> <a href="https://hubs.ly/Q02FKw5X0" target="_blank"> <br /> <button> LEARN MORE</button> <br /> </a> <p> </p> </div> </div> </div> <p> </p> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> .protocol { width: 1170px; display: inline-block; text-align: center; } .protocol img { width: 95%; height: auto; display: block; margin-left: auto; margin-right: auto; } @media screen and (max-width: 1170px) { .protocol { width: auto; } } </style> <div class="protocol"> <h2>AAV-ITR Sequencing CAPABILITY</h2> <p>Azenta has developed a proprietary Sanger sequencing method specifically designed to sequence the inverted terminal repeat (ITR) hairpin regions of adeno-associated virus (AAV) plasmids. Our proprietary protocol prevents the abrupt reduction in sequencing signal at the start of the ITR hairpin and reads through the full length of the ITR region. <br> <em><br> AAV-ITR sequencing can be incorporated into either our sequence discovery or sequence confirmation services.<br> </em></p> <p> </p> <h3 style="text-align: center;"> <strong>ITR sequencing using standard protocol</strong></h3> <a rel="noopener noreferrer" href="https://web.genewiz.com/itr50" target="_blank"><img alt="GENEWIZ AAV-ITR Protocol" src="/-/media/Images/Graph1.ashx?la=en&hash=FE68D1FC78E00B975E5B35014A5BA26D5C59B9E4"> </a> <br> <br> <br> <h3 style="text-align: center;"><strong>ITR Sequencing using GENEWIZ's proprietary protocol</strong></h3> <a rel="noopener noreferrer" href="https://web.genewiz.com/itr50" target="_blank"><img alt="GENEWIZ AAV-ITR Protocol" src="/-/media/Images/Graph2.ashx?la=en&hash=2E7FC275D14802557FD682E6FF967052A167ED28"></a> </div> </div> <script type="text/javascript" src="/assets/plugins/counter/jquery.counterup.min.js"></script> <script language="javascript" type="text/javascript"> jQuery(document).ready(function ($) { $(".row .category").find("img").addClass("img-responsive"); }); </script> <div class='Component-Frame pull-center Multi-column-TextBullet-Layout Component-BackGround-Default margin-top-0 margin-bottom-0 padding-top-10 padding-bottom-10'> <div class="container"> <div class="headline-center"> <h2>Primer Walking Features & Benefits</h2> </div> <div class="headline-left"> </div> <div class="row category"> <div class="col-md-6 col-sm-6"> <div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-gray icon-4x fa fa-check" style="border:none;"></i></div> <div class="content-boxes-in-v3"> Industry-leading turnaround time starting at 3-5 business days for sequence confirmation </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-gray icon-4x fa fa-check" style="border:none;"></i></div> <div class="content-boxes-in-v3"> Single-stranded, double-stranded, or 4x coverage available </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-gray icon-4x fa fa-check" style="border:none;"></i></div> <div class="content-boxes-in-v3"> Can be combined with our ITR Sanger sequencing service for complete sequencing of AAV plasmids </div> </div> </div> <div class="col-md-6 col-sm-6"> <div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-gray icon-4x fa fa-check" style="border:none;"></i></div> <div class="content-boxes-in-v3"> Any size plasmid prep or PCR of difficult templates are available upon request </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-gray icon-4x fa fa-check" style="border:none;"></i></div> <div class="content-boxes-in-v3"> Dedicated Ph.D.-level support at every step with real-time project updates through our online system </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-gray icon-4x fa fa-check" style="border:none;"></i></div> <div class="content-boxes-in-v3"> Contact us to locate a drop box or pick-up service near you for free shipping </div> </div> </div> </div> </div> </div><div class="headline-center Rich-Text-Edit-Area"> <link href="https://fonts.googleapis.com/css?family=Roboto:300,400,500,700" rel="stylesheet" /> <style type="text/css"> .covid-banner1 { width: 1170px; height: auto; margin: 0 auto; background-image: url("/-/media/11036-D_Plasmid-ONT_WebBanFull-Med_Background.ashx"); background-size: cover; background-position: center; text-align: left; padding: 0px 0 0px 0; } .covid-content1 { text-align: center; } .covid-box1 h3 { color: #ffffff; text-transform: none; letter-spacing: 1px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 450; line-height: 125%; font-size: 35px; margin: 0; padding-bottom: 15px; padding-top: 25px; } .covid-box1 h4 { color: #ffffff; text-transform: none; letter-spacing: 1px; line-height: 30px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 20px; margin: 0; margin-bottom: 5px; } .covid-box1 p { color: #000000; text-transform: none; letter-spacing: 1px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 15px; margin: 0; margin-bottom: 5px; } .covid-box1 button { padding: 8px 19px; color: #826ec3; letter-spacing: 1px; background: #ffffff; border: 2px solid #ffffff; border-radius: 0px; transition: all .2s ease-in-out; font-size: 15px } .covid-box1 button:hover { cursor: pointer; transform: scale(1.1); } .covid-box1 button:focus { outline: 0; } @media only screen and (max-width: 1170px) { .covid-banner1 { width: 100%; height: auto; } .covid-content1 { width: 90%; margin: 0 auto; } } </style> <br /> <div class="covid-banner1"> <div class="covid-content1"> <div class="covid-box1"> <br /> <h3>Ensure Complete Plasmid Validation on Your First Attempt</h3> <h4>with Oxford Nanopore Sequencing</h4> <a href="https://www.azenta.com/learning-center/blog/ensure-complete-plasmid-validation-your-first-attempt-oxford-nanopore-sequencing?utm_campaign=2024_06_GEN_Sanger%3A%20Ensure%20Complete%20Plasmid%20Validation%20Blog&utm_source=EU%20Primer%20Walking%20Page&utm_medium=Full%20Banner%20Ad" target="_blank"> <br /> <button> READ BLOG</button> <br /> </a> <p> </p> </div> </div> </div> <p> </p> </div> <script language="javascript" type="text/javascript"> jQuery(document).ready(function ($) { $(".SingleIcon").find("img").addClass("img-responsive center-block"); }); </script> <div class="process-v1 icon-nav Component-BackGround-Gray margin-top-0 margin-bottom-0 padding-top-10 padding-bottom-0"> <div class="container"> <div class="headline-center"> <h2>Technical Resources</h2> <p> </p> </div> <ul class="list-unstyled row "> <li class='col-sm-6'> <a href='https://web.genewiz.com/primer-walking-faq' target='_blank'> <div class='SingleIcon headline-center Component-BackGround-Default margin-top-0 margin-bottom-0 padding-top-10 padding-bottom-0'> <div class="row"> <img src="/-/media/Images/Home/icons/Purple-Icons/chat-bubble_Purple.ashx?h=110&la=en&w=210&hash=20D4E093CF40A1D72E7CE3721F0FDEAC239B298C" alt="" width="210" height="110" /> </div> <div class="row"> <h3>Frequently Asked Questions</h3> </div> <div class="row"> </div> </div> </a> </li> <!--target="_blank"--> <li class='col-sm-6'> <a href='/Public/Resources/Sample-Submission-Guidelines/Sanger-Sequencing-Sample-Submission-Guidelines/template-discovery-services#sanger-sequence' target=''> <div class='SingleIcon headline-center Component-BackGround-Default margin-top-0 margin-bottom-0 padding-top-10 padding-bottom-0'> <div class="row"> <img src="/-/media/Images/Home/icons/Purple-Icons/Prepare_Samples_Web-Icon-Workflow_Purple.ashx?h=91&la=en&w=91&hash=46CD2A5C3BAF8BA51A6B26A29368BBB9171BDA5C" alt="Building" width="91" height="91" /> </div> <div class="row"> <h3>Sample Submission Guidelines</h3> </div> <div class="row"> </div> </div> </a> </li> <!--target=""--> </ul> <div class="row headline-center"> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> body { margin: 0; } .tech-resources-container { width: 1170px; margin: 0 auto; padding: 0 10 10 10; } .tech-resources-container h2 { text-align: center; border-bottom: none; } .tech-resource { display: flex; align-items: center; flex-direction: row; margin-bottom: 35px; } .resource { flex-grow: 1; } .resource-right h3, .resource-right p { width: 90%; margin: 0 auto; border-bottom: none; text-align: left; } .resource button { padding: 5px 15px; border: none; border-radius: 4px; background: #826ec3; color: #fff; font-size: 15px; font-weight: lighter; letter-spacing: 1px; margin-top: 15px; -webkit-transition: ease-in-out 0.2s; -moz-transition: ease-in-out 0.2s; -o-transition: ease-in-out 0.2s; transition: ease-in-out 0.2s; } .resource button:hover { background: #6959a1; } @media only screen and (max-width: 800px) { .tech-resource { flex-direction: column; text-align: center; } .resource-right h3, .resource-right p { text-align: center; } } @media only screen and (max-width: 1170px) { .tech-resources-container { width: 80%; margin: 0 auto; } } </style> <div style="width: 100%; background-color: #f1f0f0; padding: 10px; padding-top: -20px; margin: 0;margin-top: -20px;"> <div class="tech-resources-container"> <div class="tech-resource"> <div class="resource resource-left"> <img alt="AAV ITR Sequencing Case Study" src="/-/media/Images/Resources/Educational-Resources/AAV_ITR_Sequencing_Thumbnail.ashx?la=en&hash=347C924AF289CF2798457DCDD30AE6690FCD558D" /> </div> <div class="resource resource-right"> <h3>Tech Note | Reading Through the Inverted Terminal Repeats (ITRs) of Adeno-Associated Virus (AAV)</h3> <p> </p> <p>GENEWIZ has developed a high-quality, direct Sanger sequencing method that reads through both intact and commonly mutated inverted terminal repeat (ITR) regions of adeno-associated virus (AAV). This method is an effective tool for assessing the integrity of ITRs in AAV plasmids.<br /> <a href="https://web.genewiz.com/aav-itr-tech-note" target="_blank"> <button>Download</button> </a> </p> </div> </div> <div class="tech-resource"> <div class="resource resource-left"> <img alt="AAV ITR Sequencing Case Study" src="/-/media/Optimizing-AAV-Blog.ashx?h=160&w=220&la=en&hash=EAF73BB6285C8CE27D421DC6340D702C499323A6" style="width: 220px; height: 160px;" /> </div> <div class="resource resource-right"> <h3>Blog | Optimizing AAV Plasmid Preparation and ITR Sequencing</h3> <p> </p> <p>The adeno-associated virus (AAV) is a powerful vehicle for gene therapy; however, working with AAV vectors can be challenging. Each construct contains two inverted terminal repeat (ITR) sequences, usually 145 bp in length, that flank the gene of interest. Learn about the challenges of preparing and validating plasmid DNA for AAV vectors containing ITRs and an optimized approach to these processes.<br /> <a href="https://www.azenta.com/learning-center/blog/optimizing-aav-plasmid-preparation-and-itr-sequencing" target="_blank"> <button>Read Now</button> </a> </p> </div> </div> <div class="tech-resource"> <div class="resource resource-left"> <img alt="" src="/-/media/Sanger-Trouble-shooting-blog.ashx?h=160&w=220&la=en&hash=24FECB44F887ED8D2D363153D5842A55828C120E" style="width: 220px; height: 160px;" /> </div> <div class="resource resource-right"> <h3>Blog | Troubleshooting DNA Templates with Sanger Sequencing</h3> <p>Sanger sequencing is highly effective for testing small targeted genomic regions and for validating results from NGS. However, as with all sequencing technologies, there are limitations and needs for troubleshooting. Read on to learn about the three basic troubleshooting steps we recommend to start with.<br /> <a href="https://www.azenta.com/learning-center/blog/troubleshooting-dna-templates-sanger-sequencing" target="_blank"> <button>Read Now</button> </a> </p> </div> </div> </div> </div> </div> <script language="javascript" type="text/javascript"> jQuery(document).ready(function ($) { $(".SingleIcon").find("img").addClass("img-responsive center-block"); }); </script> <div class="process-v1 icon-nav Component-BackGround-Default row-centered margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10"> <div class="container"> <div class="headline-center"> <h2>Related Services<br></h2> <p></p> </div> <ul class="list-unstyled row "> <li class='col-sm-4 col-centered'> <a href='/Public/Services/Sanger-Sequencing/AAV-ITR' target=''> <div class='SingleIcon headline-center Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10'> <div class="row"> <img src="/-/media/Images/Home/icons/Purple-Icons/AAV_ITR_Sequencing_Service_Icon_Purple.ashx?h=100&la=en&w=110&hash=BEEB3F54DBB25D0DDBC659D9512E0CA5C5A9536C" alt="Building" width="110" height="100" /> </div> <div class="row"> <h3>AAV-ITR Sequencing</h3> </div> <div class="row"> </div> </div> </a> </li> <!--target=""--> <li class='col-sm-4 col-centered'> <a href='/Public/Services/Molecular-Genetics/cDNA-Verification' target=''> <div class='SingleIcon headline-center Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10'> <div class="row"> <img src="/-/media/Images/Home/icons/Purple-Icons/ConfirmatorySeq_Service_Icon_Purple.ashx?h=110&la=en&w=210&hash=065FF48B11A5E294E52CADD0139051ACDC9163D4" alt="Building" width="210" height="110" /> </div> <div class="row"> <h3>Confirmatory Sequencing & Cell Bank Characterization</h3> </div> <div class="row"> </div> </div> </a> </li> <!--target=""--> <li class='col-sm-4 col-centered'> <a href='/Public/Services/Plasmid-DNA-Prep' target=''> <div class='SingleIcon headline-center Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10'> <div class="row"> <img src="/-/media/Images/Home/icons/Purple-Icons/plasmid-prep-overview_110x210_Purple.ashx?h=110&la=en&w=210&hash=D542B9BF1106F547CB84A8011F635AD43B95653A" alt="Building" width="210" height="110" /> </div> <div class="row"> <h3>Standard Plasmid DNA Preparation</h3> </div> <div class="row"> </div> </div> </a> </li> <!--target=""--> </ul> <div class="row headline-center"> </div> </div> </div><!--=== Process v1 ===--> <div class="process-v1 How-To-Order Component-BackGround-Gray margin-top-0 padding-top-10 padding-bottom-10 "> <div class=" container"> <div class="headline-center"> <div class="row"> <h2>HOW TO ORDER</h2> </div> <div class="row"> *Samples must arrive at the GENEWIZ New Jersey Laboratory before 10:00 am EST to qualify for Same Day service. 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