Targeted Yield Plasmid DNA Preparation

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right; } .plasmid-content-box img { width: 400px; } .plasmid-content-box button { border: none; border-radius: 4px; background: #826ec3; padding: 10px 20px; color: #fff; font-size: 17px; letter-spacing: 1px; cursor: pointer; } .plasmid-content-box button:hover { background: #6965a1; } @media only screen and (max-width: 768px) { .headline-center { padding-top: 0; } .plasmid-new-container { text-align: center; margin-top: 0; } .plasmid-new-content { width: 90%; margin: 0 auto; } .plasmid-new-content { display: inline-block; } #plasmid-content-box-right, #plasmid-content-box-left { width: 100%; text-align: center; } #plasmid-content-box-right { margin-top: 20px; } } @media only screen and (max-width: 1170px) { .plasmid-new-content { width: 90%; margin: 0 auto; } .plasmid-content-box img { width: 350px; } } </style> <div class="plasmid-new-container"> <div class="plasmid-new-content"> <div class="plasmid-content-box" id="plasmid-content-box-left"> <h1>TARGETED YIELD PLASMID DNA PREPARATION</h1> <div> </div> <p>Our premium Targeted Yield service delivers specific quantities of high quality, transfection-grade DNA that you can count on. This service is intended for stable, high-copy plasmids, and includes a free upgrade to an endo-free process with sequence confirmation, restriction digest, and sterility testing. We now offer large-scale, high-quality plasmids ranging from 250 µg to 5 grams, ideal for cell and gene therapy, drug discovery, and vaccine research.</p> <br /> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://clims4.genewiz.com/DNAPrep?lobId=5&serviceId=520" target="_blank"><button>Place an Order</button></a> </div> <div class="plasmid-content-box" id="plasmid-content-box-right"> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://hubs.ly/Q02Y31bh0" target="_blank"> <img alt="" src="/-/media/15019-D_Boost-Plasmid-Prep_WebBanRight.ashx?la=en&hash=4F034BAE891C7FC33CF9D6AAC03B3310BDE55D9C" /></a> </div> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <p style="text-align: center;"> </p> <style> .table-responsive { overflow-x: hidden} .table>tbody>tr>td { vertical-align: middle; } </style> <p> </p> <div class="container margin-top-50 margin-bottom-50" style="text-align: center;"> <h1>Service & Analysis Options</h1> <p style="text-align: left;"><em class="BlueCheck icon-4x fa fa-check" style="font-size: 20px; float: none; vertical-align: middle;"></em> = Included</p> <p style="text-align: left;"><em class="BlueCheck icon-4x fa fa-plus" style="font-size: 20px; float: none; vertical-align: middle; color: black;"></em> = Add-on</p> <div class="table-responsive"> <table class="table table-bordered"> <tbody class="panel panel-purple"> <tr class="panel-heading"> <td> </td> <td> </td> <td> Targeted Yield (Amount Based)</td> </tr> <tr> <td rowspan="7"> <p>Service Options</p> </td> <td>Turnaround Time</td> <td>Starting from 4-5 business days</td> </tr> <tr> <td>Plasmids Accepted</td> <td> <ul style="width: auto; display: inline-block;"> <li style="text-align: left;">High-copy</li> <li style="text-align: left;">Stable</li> </ul> </td> </tr> <tr> <td>Spectrophotometric DNA Quality Analysis</td> <td><em class="BlueCheck icon-4x fa fa-check" style="font-size: 30px; float: none;"></em></td> </tr> <tr> <td>Targeted Yield Verification</td> <td><em class="BlueCheck icon-4x fa fa-check" style="font-size: 30px; float: none;"></em></td> </tr> <tr> <td>Transfection-Grade (Endotoxin Free)</td> <td><em class="BlueCheck icon-4x fa fa-check" style="font-size: 30px; float: none;"></em></td> </tr> <tr> <td>DNA Normalization</td> <td><em class="BlueCheck icon-4x fa fa-plus" style="font-size: 30px; float: none; color: black;"></em></td> </tr> <tr> <td>Aliquots or Custom Tubes</td> <td><em class="BlueCheck icon-4x fa fa-plus" style="font-size: 30px; float: none; color: black;"></em></td> </tr> <tr> <td rowspan="5">Analysis Options </td> <td>DNA Sequencing<span style="color: #ff0000;">*</span></td> <td><em class="BlueCheck icon-4x fa fa-check" style="font-size: 30px; float: none;"></em></td> </tr> <tr> <td>Sequence Consensus Assembly and Analysis </td> <td><em class="BlueCheck icon-4x fa fa-check" style="font-size: 30px; float: none;"></em></td> </tr> <tr> <td>Full-Service DNA Sequence Confirmation</td> <td><em class="BlueCheck icon-4x fa fa-plus" style="font-size: 30px; float: none; color: black;"></em></td> </tr> <tr> <td>Restriction Digest and Agarose Gel Analysis<span style="color: #ff0000;">*</span></td> <td><em class="BlueCheck icon-4x fa fa-check" style="font-size: 30px; float: none;"></em></td> </tr> <tr> <td>Sterility Testing</td> <td><em class="BlueCheck icon-4x fa fa-check" style="font-size: 30px; float: none;"></em></td> </tr> </tbody> </table> <p style="text-align: center;"><span style="color: #ff0000;">*</span>Includes one reaction per plasmid; added reactions available as an add-on.</p> <h3>Need your prep faster? Have an unstable, low copy vector? Check out our <a href="https://www.genewiz.com/Public/Services/Plasmid-DNA-Prep/Standard">Standard-Scale (Per Run) Service</a>. </h3> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <div class="container"> <h2>TARGETED YIELDS AVAILABLE</h2> <div class="row"> <div class="col-md-10 col-centered"> <table class="table table-responsive table-bordered"> <tbody class="panel panel-purple"> <tr class="panel-heading"> <td colspan="7">Targeted Yield Service (Amount-Based) Now offering 100 mg to 5 g yields!</td> </tr> <tr> <td rowspan="2">Available Yields for High-Copy, Stable Plasmids<span style="color: #ff0000;">*</span></td> <td><span style="text-align: center;">250 µg</span></td> <td><span style="text-align: center;">1 mg</span></td> <td><span style="text-align: center;">5 mg</span></td> <td><span style="text-align: center;">10 mg</span></td> <td><span style="text-align: center;">20 mg</span></td> <td><span style="text-align: center;">100 mg</span></td> </tr> <tr> <td><span style="text-align: center;">250 mg</span></td> <td><span style="text-align: center;">500 g</span></td> <td><span style="text-align: center;">1 g</span></td> <td><span style="text-align: center;">3 g</span></td> <td><span style="text-align: center;">4 g</span></td> <td><span style="text-align: center;">5 g</span></td> </tr> </tbody> </table> </div> </div> </div> <p><span style="color: #ff0000;">*</span>Actual yield will vary with the nature of the plasmid, bacterial hosts, and other factors.</p> </div> <div class="headline-center Rich-Text-Edit-Area"> <div class="process-v1 Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10"> <div class="container"> <div class="row"> <h2>Choice of Starting Material </h2> </div> <div class="row"> <div class="col-sm-4"> <img style="height: 110px; width: 210px;" class="img-responsive center-block" alt="Building" src="~/media/Images/Services/Plasmid/icons/110x210/dna_startingmaterial_110x210.ashx?h=110&la=en&w=210&hash=562C88D5588FAA4B7DFC1068FAE3D0C691F9356C&hash=562C88D5588FAA4B7DFC1068FAE3D0C691F9356C"> <h3>DNA</h3> <p>Transformation provided as a service<span style="color: #ff0000;">*</span></p> </div> <div class="col-sm-4"> <img style="height: 110px; width: 210px;" class="img-responsive center-block" alt="Building" src="~/media/Images/Services/Plasmid/icons/110x210/glycerol-stock_startingmaterial_110x210.ashx?h=110&la=en&w=210&hash=AE1077914CF9951C0A438718253A277D1BDC18E2&hash=AE1077914CF9951C0A438718253A277D1BDC18E2"> <h3>Glycerol Stock</h3> <p>Provide 200 μL frozen stock</p> </div> <div class="col-sm-4"> <img style="height: 110px; width: 210px;" class="img-responsive center-block" alt="Building" src="~/media/Images/Services/Plasmid/icons/110x210/bacterial-colony-startingmaterial_110x210.ashx?h=110&la=en&w=210&hash=16C5760CA290E6B2BA19088A39D1A2FC62630DDA&hash=16C5760CA290E6B2BA19088A39D1A2FC62630DDA"> <h3>Bacterial Colony</h3> <p>Please provide fresh colonies (<3 days old)</p> </div> </div> <div class="row margin-top-30"> <p class="text-left"><span style="color: #ff0000;">*</span>For DNA samples, Azenta Life Sciences, formerly GENEWIZ provides multiple host strains to choose from for transformation. For custom strains, please provide the starting material as bacterial colony or clonal glycerol stock, or select “Other” for host strain when placing your order and include the competent cells with your DNA submission.</p> </div> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> .body { text-align: center; display: inline-block; } .banner1 { width: 1170px; background-image: url('/-/media/10013-D_Customer-Referral_WebBanFull-Med_Background1.ashx'); background-size: cover; text-align: center; display: flex; padding: 10px; } .banner1 img { text-align: center; display: block; margin-left: auto; margin-right: auto; } #banner1-left { width: 60%; padding: 5px; } #banner1-right { width: 35%; padding: 15px; } #banner1-left h1 { text-transform: none; letter-spacing: 1px; line-height: 30px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 40px; margin: 0; margin-top: 15px; margin-bottom: 20px; color: #000000; } #banner1-left h3 { text-transform: none; 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}); </script> <div class="process-v1 icon-nav Component-BackGround-Default margin-top-30 margin-bottom-10 padding-top-0 padding-bottom-0"> <div class="container"> <div class="headline-center"> <h1>Technical Resources</h1> <p></p> </div> <ul class="list-unstyled row "> <li class='col-sm-6'> <a href='/Public/Resources/FAQs/FAQs-Plasmid-Prep' target='_blank'> <div class='SingleIcon headline-center Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-0 padding-bottom-0'> <div class="row"> <img src="/-/media/Images/Home/icons/Purple-Icons/chat-bubble_Purple.ashx?h=110&la=en&w=210&hash=20D4E093CF40A1D72E7CE3721F0FDEAC239B298C" alt="Building" width="210" height="110" /> </div> <div class="row"> <h3>Frequently Asked Questions</h3> </div> <div class="row"> </div> </div> </a> </li>  <li class='col-sm-6'> <a href='https://www.genewiz.com/en/Public/Resources/Sample-Submission-Guidelines/Plasmid-Preparation-Sample-Submission-Guidelines' target='_blank'> <div class='SingleIcon headline-center Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-0 padding-bottom-0'> <div class="row"> <img src="/-/media/Images/Home/icons/Purple-Icons/Prepare_Samples_Web-Icon-Workflow_Purple.ashx?h=91&la=en&w=91&hash=46CD2A5C3BAF8BA51A6B26A29368BBB9171BDA5C" alt="Building" width="91" height="91" /> </div> <div class="row"> <h3>Sample Submission Guidelines</h3> </div> <div class="row"> </div> </div> </a> </li>  </ul> <div class="row headline-center"> </div> </div> </div><div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> body { margin: 0; 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margin: 0 auto; } } </style> <div style="width: 100%; background-color: #00000; padding: 20px; margin: 0;margin-top: -20px;"> <h2></h2> <div class="tech-resources-container"> <div class="tech-resource"> <div class="resource resource-left"> <img alt="Tech Note" src="/-/media/Plasmid-Prep-Tech-Note-Thumbnail.ashx?h=160&w=220&la=en&hash=F37CE1AD4FC30DCAB54FD0C2849F373956556CE4" /> </div> <div class="resource resource-right"> <h3>Tech Note | Optimizing Plasmid Preparation to Increase Yield and Reduce Endotoxins</h3> <p> </p> <p>Plasmid DNA (pDNA) is vital for a range of biopharmaceutical and biotechnological applications but maximizing yield while minimizing endotoxins can be challenging. This tech note explores strategies to optimize bacterial growth conditions, enhance lysis, and refine purification protocols to boost pDNA yield and purity, ensuring consistent, high-quality results.<br /> <a href="https://web.genewiz.com/technote/optimizingplasmidprep" target="_blank"> <button>Download Now</button> </a> </p> </div> </div> </div> </div> </div> <script language="javascript" type="text/javascript"> jQuery(document).ready(function ($) { $(".SingleIcon").find("img").addClass("img-responsive center-block"); }); </script> <div class="process-v1 icon-nav Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10"> <div class="container"> <div class="headline-center"> <h2>Related Services</h2> <p></p> </div> <ul class="list-unstyled row row row-centered"> <li class='col-sm-3'> <a href='/Public/Services/Oligo' target='blank'> <div class='SingleIcon headline-center Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 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target='blank'> <div class='SingleIcon headline-center Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10'> <div class="row"> <img src="/-/media/Images/Home/icons/Purple-Icons/direct-colony-sequencing-110x210_Purple.ashx?h=110&la=en&w=210&hash=2BD6B78E3AC9A55AF5FA2D9CAE3D274478F9384D" alt="Building" width="210" height="110" /> </div> <div class="row"> <h3>Direct Colony Sequencing</h3> </div> <div class="row"> </div> </div> </a> </li>  <li class='col-sm-3'> <a href='/Public/Services/Gene-Synthesis' target='blank'> <div class='SingleIcon headline-center Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10'> <div class="row"> <img src="/-/media/Images/Home/icons/Purple-Icons/gene-synthesis_110x210_Purple.ashx?h=110&la=en&w=210&hash=61237659C1B6D3300B1F2AB38BCEFFCBCDE7F7A3" alt="Building" width="210" height="110" /> </div> <div class="row"> <h3>Gene Synthesis</h3> </div> <div class="row"> </div> </div> </a> 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