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Himisha Dixit - Academia.edu

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class="ds2-5-button hidden profile-cta-button grow js-profile-unfollow-button" data-broccoli-component="user-info.unfollow-button" data-click-track="profile-user-info-unfollow-button" data-unfollow-user-id="2800530"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">done</span>Following</button></div></div><div class="user-stats-container"><a><div class="stat-container js-profile-followers"><p class="label">Followers</p><p class="data">6</p></div></a><a><div class="stat-container js-profile-followees" data-broccoli-component="user-info.followees-count" data-click-track="profile-expand-user-info-following"><p class="label">Following</p><p class="data">1</p></div></a><a><div class="stat-container js-profile-coauthors" data-broccoli-component="user-info.coauthors-count" data-click-track="profile-expand-user-info-coauthors"><p class="label">Co-author</p><p class="data">1</p></div></a><span><div class="stat-container"><p class="label"><span class="js-profile-total-view-text">Public Views</span></p><p class="data"><span class="js-profile-view-count"></span></p></div></span></div><div class="user-bio-container"><div class="profile-bio fake-truncate js-profile-about" style="margin: 0px;">Biotechnology, Computational Biology<br /><div class="js-profile-less-about u-linkUnstyled u-tcGrayDarker u-textDecorationUnderline u-displayNone">less</div></div></div><div class="ri-section"><div class="ri-section-header"><span>Interests</span></div><div class="ri-tags-container"><a data-click-track="profile-user-info-expand-research-interests" data-has-card-for-ri-list="2800530" href="https://www.academia.edu/Documents/in/Microbiology_and_Biotechnology"><div id="js-react-on-rails-context" style="display:none" 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class="uploads-container" id="social-redesign-work-container"><div class="upload-header"><h2 class="ds2-5-heading-sans-serif-xs">Uploads</h2></div><div class="documents-container backbone-social-profile-documents" style="width: 100%;"><div class="u-taCenter"></div><div class="profile--tab_content_container js-tab-pane tab-pane active" id="all"><div class="profile--tab_heading_container js-section-heading" data-section="Papers" id="Papers"><h3 class="profile--tab_heading_container">Papers by Himisha Dixit</h3></div><div class="js-work-strip profile--work_container" data-work-id="71844827"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/71844827/Deciphering_Diversity_at_er_Loci_for_Diversification_of_Powdery_Mildew_Resistance_in_Pea"><img alt="Research paper thumbnail of Deciphering Diversity at er Loci for Diversification of Powdery Mildew Resistance in Pea" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/71844827/Deciphering_Diversity_at_er_Loci_for_Diversification_of_Powdery_Mildew_Resistance_in_Pea">Deciphering Diversity at er Loci for Diversification of Powdery Mildew Resistance in Pea</a></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Agricultural biotechnology aims to scrutinize the field crops which feed amongst half of the worl...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Agricultural biotechnology aims to scrutinize the field crops which feed amongst half of the world鈥檚 population by improving their agronomic traits using various biotechnological tools. Pea- an important cash crop, rich in nutrients, but frequently infected with powdery mildew (fungal disease) that destroy the whole crop which causes the economy loss for growers. We therefore, targeted this research to find the pathogen resistant pea lines and further decipher the diversity at er locus among resistant pea lines. Screening for resistant pea lines was done with Erysiphe pisi isolates (Genebank submission: KX45 5922.1) under net house and green house conditions. Molecular studies revealed that Erysiphe resistant (er1) gene was present in 40 lines out of selected 50 pea lines and the mutational character was conferred up to 36 genotypes with 11 haplotype groups. The haplotype (gene) diversity (Hd) was found to be 0.5571卤0.099 SD and the nucleotide diversity (Pi) was 0.0160卤0.0042 SD Maj...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="71844827"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="71844827"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 71844827; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=71844827]").text(description); $(".js-view-count[data-work-id=71844827]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 71844827; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='71844827']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 71844827, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=71844827]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":71844827,"title":"Deciphering Diversity at er Loci for Diversification of Powdery Mildew Resistance in Pea","translated_title":"","metadata":{"abstract":"Agricultural biotechnology aims to scrutinize the field crops which feed amongst half of the world鈥檚 population by improving their agronomic traits using various biotechnological tools. Pea- an important cash crop, rich in nutrients, but frequently infected with powdery mildew (fungal disease) that destroy the whole crop which causes the economy loss for growers. We therefore, targeted this research to find the pathogen resistant pea lines and further decipher the diversity at er locus among resistant pea lines. Screening for resistant pea lines was done with Erysiphe pisi isolates (Genebank submission: KX45 5922.1) under net house and green house conditions. Molecular studies revealed that Erysiphe resistant (er1) gene was present in 40 lines out of selected 50 pea lines and the mutational character was conferred up to 36 genotypes with 11 haplotype groups. The haplotype (gene) diversity (Hd) was found to be 0.5571卤0.099 SD and the nucleotide diversity (Pi) was 0.0160卤0.0042 SD Maj...","publisher":"Research Square Platform LLC"},"translated_abstract":"Agricultural biotechnology aims to scrutinize the field crops which feed amongst half of the world鈥檚 population by improving their agronomic traits using various biotechnological tools. Pea- an important cash crop, rich in nutrients, but frequently infected with powdery mildew (fungal disease) that destroy the whole crop which causes the economy loss for growers. We therefore, targeted this research to find the pathogen resistant pea lines and further decipher the diversity at er locus among resistant pea lines. Screening for resistant pea lines was done with Erysiphe pisi isolates (Genebank submission: KX45 5922.1) under net house and green house conditions. Molecular studies revealed that Erysiphe resistant (er1) gene was present in 40 lines out of selected 50 pea lines and the mutational character was conferred up to 36 genotypes with 11 haplotype groups. The haplotype (gene) diversity (Hd) was found to be 0.5571卤0.099 SD and the nucleotide diversity (Pi) was 0.0160卤0.0042 SD Maj...","internal_url":"https://www.academia.edu/71844827/Deciphering_Diversity_at_er_Loci_for_Diversification_of_Powdery_Mildew_Resistance_in_Pea","translated_internal_url":"","created_at":"2022-02-19T05:19:17.918-08:00","preview_url":null,"current_user_can_edit":false,"current_user_is_owner":null,"owner_id":2800530,"coauthors_can_edit":false,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Deciphering_Diversity_at_er_Loci_for_Diversification_of_Powdery_Mildew_Resistance_in_Pea","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":2800530,"first_name":"Himisha","middle_initials":null,"last_name":"Dixit","page_name":"HimishaDixit","domain_name":"independent","created_at":"2012-11-22T17:04:28.038-08:00","display_name":"Himisha Dixit","url":"https://independent.academia.edu/HimishaDixit"},"attachments":[],"research_interests":[],"urls":[{"id":17801592,"url":"https://www.researchsquare.com/article/rs-1239249/v1"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="34614828"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/34614828/In_vitro_propagation_of_a_medicinal_important_plant_Bacopa_monnieri_from_nodal_explants"><img alt="Research paper thumbnail of In vitro propagation of a medicinal important plant Bacopa monnieri from nodal explants" class="work-thumbnail" src="https://attachments.academia-assets.com/54480222/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/34614828/In_vitro_propagation_of_a_medicinal_important_plant_Bacopa_monnieri_from_nodal_explants">In vitro propagation of a medicinal important plant Bacopa monnieri from nodal explants</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/Himidixit">Himi dixit</a> and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/HimishaDixit">Himisha Dixit</a></span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Bacopa monnieri, a traditional Indian medicinal plant with high commercial importance, is used as...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Bacopa monnieri, a traditional Indian medicinal plant with high commercial importance, is used as a neurotonic, immuno modulator, adaptogen transquilizing, memory and learning enhancing, cerebral activator, anti-ulcer, antispasmodic, anti-asthmatic ayurvedic herb. The study was undertaken in order to elaborate the tissue culture technique for Brahmi (Bacopa monnieri). In the present study nodal explants were taken for in vitro propagation. For sterilization, 0.01% HgCl2 was used as the sterilant. The explants gave best results when treated with the sterilant for 5 minutes. To induce bud-break, nodal segments were inoculated onto MS media supplemented with 1mg/l BAP. Basal MS medium without any plant growth hormone was used as control. Further multiplication was obtained on MS medium + BAP (1.0 mg/l) +IAA (0.5 mg/l). The shoots were rooted and best rooting was observed by IBA when incorporated in MS at different concentrations (0.1-0.3 mg/l) and 0.2 mg/l gave good results. After 30 days about 2.2 cm length was observed. Almost 70% of the rooted shoots survived during hardening.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="84417fe3f3e46dc66f6eddd8010a7708" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{&quot;attachment_id&quot;:54480222,&quot;asset_id&quot;:34614828,&quot;asset_type&quot;:&quot;Work&quot;,&quot;button_location&quot;:&quot;profile&quot;}" href="https://www.academia.edu/attachments/54480222/download_file?st=MTczMjg1NTIyNSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="34614828"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="34614828"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 34614828; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=34614828]").text(description); $(".js-view-count[data-work-id=34614828]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 34614828; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='34614828']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 34614828, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "84417fe3f3e46dc66f6eddd8010a7708" } } $('.js-work-strip[data-work-id=34614828]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":34614828,"title":"In vitro propagation of a medicinal important plant Bacopa monnieri from nodal explants","translated_title":"","metadata":{"abstract":"Bacopa monnieri, a traditional Indian medicinal plant with high commercial importance, is used as a neurotonic, immuno modulator, adaptogen transquilizing, memory and learning enhancing, cerebral activator, anti-ulcer, antispasmodic, anti-asthmatic ayurvedic herb. The study was undertaken in order to elaborate the tissue culture technique for Brahmi (Bacopa monnieri). In the present study nodal explants were taken for in vitro propagation. For sterilization, 0.01% HgCl2 was used as the sterilant. The explants gave best results when treated with the sterilant for 5 minutes. To induce bud-break, nodal segments were inoculated onto MS media supplemented with 1mg/l BAP. Basal MS medium without any plant growth hormone was used as control. Further multiplication was obtained on MS medium + BAP (1.0 mg/l) +IAA (0.5 mg/l). The shoots were rooted and best rooting was observed by IBA when incorporated in MS at different concentrations (0.1-0.3 mg/l) and 0.2 mg/l gave good results. After 30 days about 2.2 cm length was observed. Almost 70% of the rooted shoots survived during hardening."},"translated_abstract":"Bacopa monnieri, a traditional Indian medicinal plant with high commercial importance, is used as a neurotonic, immuno modulator, adaptogen transquilizing, memory and learning enhancing, cerebral activator, anti-ulcer, antispasmodic, anti-asthmatic ayurvedic herb. The study was undertaken in order to elaborate the tissue culture technique for Brahmi (Bacopa monnieri). In the present study nodal explants were taken for in vitro propagation. For sterilization, 0.01% HgCl2 was used as the sterilant. The explants gave best results when treated with the sterilant for 5 minutes. To induce bud-break, nodal segments were inoculated onto MS media supplemented with 1mg/l BAP. Basal MS medium without any plant growth hormone was used as control. Further multiplication was obtained on MS medium + BAP (1.0 mg/l) +IAA (0.5 mg/l). The shoots were rooted and best rooting was observed by IBA when incorporated in MS at different concentrations (0.1-0.3 mg/l) and 0.2 mg/l gave good results. After 30 days about 2.2 cm length was observed. Almost 70% of the rooted shoots survived during hardening.","internal_url":"https://www.academia.edu/34614828/In_vitro_propagation_of_a_medicinal_important_plant_Bacopa_monnieri_from_nodal_explants","translated_internal_url":"","created_at":"2017-09-19T23:37:39.590-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":68471456,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":30316046,"work_id":34614828,"tagging_user_id":68471456,"tagged_user_id":2800530,"co_author_invite_id":null,"email":"h***t@gmail.com","display_order":1,"name":"Himisha Dixit","title":"In vitro propagation of a medicinal important plant Bacopa monnieri from nodal explants"}],"downloadable_attachments":[{"id":54480222,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/54480222/thumbnails/1.jpg","file_name":"ijrpb_51_1_Himisha_Dixit_1-4_1.pdf","download_url":"https://www.academia.edu/attachments/54480222/download_file?st=MTczMjg1NTIyNSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"In_vitro_propagation_of_a_medicinal_impo.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/54480222/ijrpb_51_1_Himisha_Dixit_1-4_1-libre.pdf?1505889526=\u0026response-content-disposition=attachment%3B+filename%3DIn_vitro_propagation_of_a_medicinal_impo.pdf\u0026Expires=1732858824\u0026Signature=VJsKrKg9s-yxQV1XeYZqzvRSzhzVb2KW9Vg6CYJx7CT5oe4rVmQ83FWeeRj-zCh2BAOjpLbOSS1v2f6tfDzZjPa5LyL-mkxZPf1IKE2gmN4GoAPxfsUsCCfDTxPdZBFjx7lPqO6He7UknQF1wxwrfw555DC2KgCNLm0qeNk9inycl7uf8ilhBQ2CxPEJmtCkl2AFHzbxU~wU8XGi9cQyGWw3Xd3XvpvJS0RNn6lAf~TgVWWKK9YugsVKkswKJaFSK8th1A-WoRwdbGoui~8cahvCV2zgA9PJ~JUrskoF9-wg8X4ECO-geAZet1EDqrctvjzjo~Xb7a8~E7m7sko5wg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"In_vitro_propagation_of_a_medicinal_important_plant_Bacopa_monnieri_from_nodal_explants","translated_slug":"","page_count":4,"language":"en","content_type":"Work","owner":{"id":68471456,"first_name":"Himi","middle_initials":null,"last_name":"dixit","page_name":"Himidixit","domain_name":"independent","created_at":"2017-09-19T23:36:23.471-07:00","display_name":"Himi dixit","url":"https://independent.academia.edu/Himidixit"},"attachments":[{"id":54480222,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/54480222/thumbnails/1.jpg","file_name":"ijrpb_51_1_Himisha_Dixit_1-4_1.pdf","download_url":"https://www.academia.edu/attachments/54480222/download_file?st=MTczMjg1NTIyNSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"In_vitro_propagation_of_a_medicinal_impo.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/54480222/ijrpb_51_1_Himisha_Dixit_1-4_1-libre.pdf?1505889526=\u0026response-content-disposition=attachment%3B+filename%3DIn_vitro_propagation_of_a_medicinal_impo.pdf\u0026Expires=1732858825\u0026Signature=Vx2YdGtw4y29jOoSye8TsAaqURK0-J6gpp2SIHFyCepepz3BN9hiuXmlSxKWBNS51VGU7015MN-WzdE~~W7EfFOUQkrfNS6oZ1a2g9kZlmkCzVj7kXsI0sEUUAAxpCFXlGl5H3izoIKH6smuc0n4YtpRrEcD5lHXnIv5tdHXqummt7YOI3imgnOf79JGP66DwOKkT1PadznEpXxLEo~yvpmpxAgLb-pTx-m6lGdd9RsUF6ENezkd7gI1xX-qws0Vp-pOS6IS-DcRGg7XaZOJOMNF2UfDZicg~skJp9zu08NExKDdhROsbXRYtVqtuvK9M~fxWkJIzzDy6hf9cc1JAA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":68,"name":"Bioengineering","url":"https://www.academia.edu/Documents/in/Bioengineering"},{"id":140,"name":"Pharmacology","url":"https://www.academia.edu/Documents/in/Pharmacology"},{"id":656,"name":"Pharmacy","url":"https://www.academia.edu/Documents/in/Pharmacy"},{"id":1290,"name":"Immunology","url":"https://www.academia.edu/Documents/in/Immunology"},{"id":2513,"name":"Molecular Biology","url":"https://www.academia.edu/Documents/in/Molecular_Biology"},{"id":5398,"name":"Biotechnology","url":"https://www.academia.edu/Documents/in/Biotechnology"},{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="40834745"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/40834745/Antimicrobial_and_phytochemical_analysis_of_some_indigenous_plants"><img alt="Research paper thumbnail of Antimicrobial and phytochemical analysis of some indigenous plants" class="work-thumbnail" src="https://attachments.academia-assets.com/61119740/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/40834745/Antimicrobial_and_phytochemical_analysis_of_some_indigenous_plants">Antimicrobial and phytochemical analysis of some indigenous plants</a></div><div class="wp-workCard_item"><span>World Researchers Associations</span><span>, 2019</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The antimicrobial activity of the extracts of the Calotropis procera, Datura metel, Cuscuta refle...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The antimicrobial activity of the extracts of the Calotropis procera, Datura metel, Cuscuta reflexa, Pongamia pinnata and Nerium oleander has been studied against the pathogenic bacteria Staphylococcus aureus, Escherichia coli, Pseudomonas aureuginosa, Klebsiella pneumoiniae, Paenibacillus and Bacilus subtilis. Significant antibacterial activity was observed with extract of ethanol, methanol and chloroform. The ethanol extract of D. metel showed maximum antibacterial activity against the all tested bacteria except the P. aureuginosa. Aqueous extract showed no measurable antibacterial activity. Inhibition was seen as concentration dependent phenomenon. Phytochemical screening revealed the presence of alkaloids, flavanoids, glycosides, steroids, tannins, phenol and saponin.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="20f03f4380a26fdcf3e2fcbd63800d50" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{&quot;attachment_id&quot;:61119740,&quot;asset_id&quot;:40834745,&quot;asset_type&quot;:&quot;Work&quot;,&quot;button_location&quot;:&quot;profile&quot;}" href="https://www.academia.edu/attachments/61119740/download_file?st=MTczMjg1NTIyNSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="40834745"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="40834745"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 40834745; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=40834745]").text(description); $(".js-view-count[data-work-id=40834745]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 40834745; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='40834745']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 40834745, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "20f03f4380a26fdcf3e2fcbd63800d50" } } $('.js-work-strip[data-work-id=40834745]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":40834745,"title":"Antimicrobial and phytochemical analysis of some indigenous plants","translated_title":"","metadata":{"abstract":"The antimicrobial activity of the extracts of the Calotropis procera, Datura metel, Cuscuta reflexa, Pongamia pinnata and Nerium oleander has been studied against the pathogenic bacteria Staphylococcus aureus, Escherichia coli, Pseudomonas aureuginosa, Klebsiella pneumoiniae, Paenibacillus and Bacilus subtilis. Significant antibacterial activity was observed with extract of ethanol, methanol and chloroform. The ethanol extract of D. metel showed maximum antibacterial activity against the all tested bacteria except the P. aureuginosa. Aqueous extract showed no measurable antibacterial activity. Inhibition was seen as concentration dependent phenomenon. Phytochemical screening revealed the presence of alkaloids, flavanoids, glycosides, steroids, tannins, phenol and saponin.","publication_date":{"day":null,"month":null,"year":2019,"errors":{}},"publication_name":"World Researchers Associations"},"translated_abstract":"The antimicrobial activity of the extracts of the Calotropis procera, Datura metel, Cuscuta reflexa, Pongamia pinnata and Nerium oleander has been studied against the pathogenic bacteria Staphylococcus aureus, Escherichia coli, Pseudomonas aureuginosa, Klebsiella pneumoiniae, Paenibacillus and Bacilus subtilis. Significant antibacterial activity was observed with extract of ethanol, methanol and chloroform. The ethanol extract of D. metel showed maximum antibacterial activity against the all tested bacteria except the P. aureuginosa. Aqueous extract showed no measurable antibacterial activity. Inhibition was seen as concentration dependent phenomenon. Phytochemical screening revealed the presence of alkaloids, flavanoids, glycosides, steroids, tannins, phenol and saponin.","internal_url":"https://www.academia.edu/40834745/Antimicrobial_and_phytochemical_analysis_of_some_indigenous_plants","translated_internal_url":"","created_at":"2019-11-04T01:45:12.779-08:00","preview_url":null,"current_user_can_edit":false,"current_user_is_owner":null,"owner_id":2800530,"coauthors_can_edit":false,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":61119740,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/61119740/thumbnails/1.jpg","file_name":"Dixit_201920191104-60089-zz9uhb.pdf","download_url":"https://www.academia.edu/attachments/61119740/download_file?st=MTczMjg1NTIyNSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Antimicrobial_and_phytochemical_analysis.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/61119740/Dixit_201920191104-60089-zz9uhb-libre.pdf?1572862422=\u0026response-content-disposition=attachment%3B+filename%3DAntimicrobial_and_phytochemical_analysis.pdf\u0026Expires=1732858825\u0026Signature=GqjbX4ssfHpVPp9ifETVG~qEpenscClG8-3mJY9yrXjnIjqBnOPiNOhjKXsQStuV9v5gBg92gaQDPKxmRZQc087MeiVg9P1eSrF5ZJ9x5kynC2RDNGzNnbBHxwVIKh2yVmp3V7eyGmCptY6z~kShUhwO5tOqAAC1pKkhDx~X51uYFcB3oBh-2tuZaYBM4Lk7HLvyNdptXhIhHeJ3Im~CtGgEhRewYuQsxIMiOgVJGL7hQpl2Npu4HcWsQjTHwp8Q2B9sgsIOLgPceOC8PzmNW4nGeg6NVbukL393jjz~u-jJ-CnS~BtFZD72VUJiNah-ZRyFfQkGAcgpZT1vLDqfZQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Antimicrobial_and_phytochemical_analysis_of_some_indigenous_plants","translated_slug":"","page_count":8,"language":"en","content_type":"Work","owner":{"id":2800530,"first_name":"Himisha","middle_initials":null,"last_name":"Dixit","page_name":"HimishaDixit","domain_name":"independent","created_at":"2012-11-22T17:04:28.038-08:00","display_name":"Himisha Dixit","url":"https://independent.academia.edu/HimishaDixit"},"attachments":[{"id":61119740,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/61119740/thumbnails/1.jpg","file_name":"Dixit_201920191104-60089-zz9uhb.pdf","download_url":"https://www.academia.edu/attachments/61119740/download_file?st=MTczMjg1NTIyNSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Antimicrobial_and_phytochemical_analysis.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/61119740/Dixit_201920191104-60089-zz9uhb-libre.pdf?1572862422=\u0026response-content-disposition=attachment%3B+filename%3DAntimicrobial_and_phytochemical_analysis.pdf\u0026Expires=1732858825\u0026Signature=GqjbX4ssfHpVPp9ifETVG~qEpenscClG8-3mJY9yrXjnIjqBnOPiNOhjKXsQStuV9v5gBg92gaQDPKxmRZQc087MeiVg9P1eSrF5ZJ9x5kynC2RDNGzNnbBHxwVIKh2yVmp3V7eyGmCptY6z~kShUhwO5tOqAAC1pKkhDx~X51uYFcB3oBh-2tuZaYBM4Lk7HLvyNdptXhIhHeJ3Im~CtGgEhRewYuQsxIMiOgVJGL7hQpl2Npu4HcWsQjTHwp8Q2B9sgsIOLgPceOC8PzmNW4nGeg6NVbukL393jjz~u-jJ-CnS~BtFZD72VUJiNah-ZRyFfQkGAcgpZT1vLDqfZQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":15019,"name":"Medicinal Plants","url":"https://www.academia.edu/Documents/in/Medicinal_Plants"},{"id":67190,"name":"Phytochemical Screening of Medicinal Plants","url":"https://www.academia.edu/Documents/in/Phytochemical_Screening_of_Medicinal_Plants"},{"id":73418,"name":"Medicinal plants and natural products","url":"https://www.academia.edu/Documents/in/Medicinal_plants_and_natural_products"},{"id":111007,"name":"Antimicrobial activity","url":"https://www.academia.edu/Documents/in/Antimicrobial_activity"},{"id":1692059,"name":"Minimum inhibitory concentration","url":"https://www.academia.edu/Documents/in/Minimum_inhibitory_concentration"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> </div><div class="profile--tab_content_container js-tab-pane tab-pane" data-section-id="9857676" id="papers"><div class="js-work-strip profile--work_container" data-work-id="71844827"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/71844827/Deciphering_Diversity_at_er_Loci_for_Diversification_of_Powdery_Mildew_Resistance_in_Pea"><img alt="Research paper thumbnail of Deciphering Diversity at er Loci for Diversification of Powdery Mildew Resistance in Pea" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/71844827/Deciphering_Diversity_at_er_Loci_for_Diversification_of_Powdery_Mildew_Resistance_in_Pea">Deciphering Diversity at er Loci for Diversification of Powdery Mildew Resistance in Pea</a></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Agricultural biotechnology aims to scrutinize the field crops which feed amongst half of the worl...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Agricultural biotechnology aims to scrutinize the field crops which feed amongst half of the world鈥檚 population by improving their agronomic traits using various biotechnological tools. Pea- an important cash crop, rich in nutrients, but frequently infected with powdery mildew (fungal disease) that destroy the whole crop which causes the economy loss for growers. We therefore, targeted this research to find the pathogen resistant pea lines and further decipher the diversity at er locus among resistant pea lines. Screening for resistant pea lines was done with Erysiphe pisi isolates (Genebank submission: KX45 5922.1) under net house and green house conditions. Molecular studies revealed that Erysiphe resistant (er1) gene was present in 40 lines out of selected 50 pea lines and the mutational character was conferred up to 36 genotypes with 11 haplotype groups. The haplotype (gene) diversity (Hd) was found to be 0.5571卤0.099 SD and the nucleotide diversity (Pi) was 0.0160卤0.0042 SD Maj...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="71844827"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="71844827"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 71844827; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=71844827]").text(description); $(".js-view-count[data-work-id=71844827]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 71844827; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='71844827']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 71844827, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=71844827]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":71844827,"title":"Deciphering Diversity at er Loci for Diversification of Powdery Mildew Resistance in Pea","translated_title":"","metadata":{"abstract":"Agricultural biotechnology aims to scrutinize the field crops which feed amongst half of the world鈥檚 population by improving their agronomic traits using various biotechnological tools. Pea- an important cash crop, rich in nutrients, but frequently infected with powdery mildew (fungal disease) that destroy the whole crop which causes the economy loss for growers. We therefore, targeted this research to find the pathogen resistant pea lines and further decipher the diversity at er locus among resistant pea lines. Screening for resistant pea lines was done with Erysiphe pisi isolates (Genebank submission: KX45 5922.1) under net house and green house conditions. Molecular studies revealed that Erysiphe resistant (er1) gene was present in 40 lines out of selected 50 pea lines and the mutational character was conferred up to 36 genotypes with 11 haplotype groups. The haplotype (gene) diversity (Hd) was found to be 0.5571卤0.099 SD and the nucleotide diversity (Pi) was 0.0160卤0.0042 SD Maj...","publisher":"Research Square Platform LLC"},"translated_abstract":"Agricultural biotechnology aims to scrutinize the field crops which feed amongst half of the world鈥檚 population by improving their agronomic traits using various biotechnological tools. Pea- an important cash crop, rich in nutrients, but frequently infected with powdery mildew (fungal disease) that destroy the whole crop which causes the economy loss for growers. We therefore, targeted this research to find the pathogen resistant pea lines and further decipher the diversity at er locus among resistant pea lines. Screening for resistant pea lines was done with Erysiphe pisi isolates (Genebank submission: KX45 5922.1) under net house and green house conditions. Molecular studies revealed that Erysiphe resistant (er1) gene was present in 40 lines out of selected 50 pea lines and the mutational character was conferred up to 36 genotypes with 11 haplotype groups. The haplotype (gene) diversity (Hd) was found to be 0.5571卤0.099 SD and the nucleotide diversity (Pi) was 0.0160卤0.0042 SD Maj...","internal_url":"https://www.academia.edu/71844827/Deciphering_Diversity_at_er_Loci_for_Diversification_of_Powdery_Mildew_Resistance_in_Pea","translated_internal_url":"","created_at":"2022-02-19T05:19:17.918-08:00","preview_url":null,"current_user_can_edit":false,"current_user_is_owner":null,"owner_id":2800530,"coauthors_can_edit":false,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Deciphering_Diversity_at_er_Loci_for_Diversification_of_Powdery_Mildew_Resistance_in_Pea","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":2800530,"first_name":"Himisha","middle_initials":null,"last_name":"Dixit","page_name":"HimishaDixit","domain_name":"independent","created_at":"2012-11-22T17:04:28.038-08:00","display_name":"Himisha Dixit","url":"https://independent.academia.edu/HimishaDixit"},"attachments":[],"research_interests":[],"urls":[{"id":17801592,"url":"https://www.researchsquare.com/article/rs-1239249/v1"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="34614828"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/34614828/In_vitro_propagation_of_a_medicinal_important_plant_Bacopa_monnieri_from_nodal_explants"><img alt="Research paper thumbnail of In vitro propagation of a medicinal important plant Bacopa monnieri from nodal explants" class="work-thumbnail" src="https://attachments.academia-assets.com/54480222/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/34614828/In_vitro_propagation_of_a_medicinal_important_plant_Bacopa_monnieri_from_nodal_explants">In vitro propagation of a medicinal important plant Bacopa monnieri from nodal explants</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/Himidixit">Himi dixit</a> and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/HimishaDixit">Himisha Dixit</a></span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Bacopa monnieri, a traditional Indian medicinal plant with high commercial importance, is used as...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Bacopa monnieri, a traditional Indian medicinal plant with high commercial importance, is used as a neurotonic, immuno modulator, adaptogen transquilizing, memory and learning enhancing, cerebral activator, anti-ulcer, antispasmodic, anti-asthmatic ayurvedic herb. The study was undertaken in order to elaborate the tissue culture technique for Brahmi (Bacopa monnieri). In the present study nodal explants were taken for in vitro propagation. For sterilization, 0.01% HgCl2 was used as the sterilant. The explants gave best results when treated with the sterilant for 5 minutes. To induce bud-break, nodal segments were inoculated onto MS media supplemented with 1mg/l BAP. Basal MS medium without any plant growth hormone was used as control. Further multiplication was obtained on MS medium + BAP (1.0 mg/l) +IAA (0.5 mg/l). The shoots were rooted and best rooting was observed by IBA when incorporated in MS at different concentrations (0.1-0.3 mg/l) and 0.2 mg/l gave good results. After 30 days about 2.2 cm length was observed. Almost 70% of the rooted shoots survived during hardening.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="84417fe3f3e46dc66f6eddd8010a7708" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{&quot;attachment_id&quot;:54480222,&quot;asset_id&quot;:34614828,&quot;asset_type&quot;:&quot;Work&quot;,&quot;button_location&quot;:&quot;profile&quot;}" href="https://www.academia.edu/attachments/54480222/download_file?st=MTczMjg1NTIyNSw4LjIyMi4yMDguMTQ2&st=MTczMjg1NTIyNSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="34614828"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="34614828"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 34614828; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=34614828]").text(description); $(".js-view-count[data-work-id=34614828]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 34614828; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='34614828']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 34614828, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "84417fe3f3e46dc66f6eddd8010a7708" } } $('.js-work-strip[data-work-id=34614828]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":34614828,"title":"In vitro propagation of a medicinal important plant Bacopa monnieri from nodal explants","translated_title":"","metadata":{"abstract":"Bacopa monnieri, a traditional Indian medicinal plant with high commercial importance, is used as a neurotonic, immuno modulator, adaptogen transquilizing, memory and learning enhancing, cerebral activator, anti-ulcer, antispasmodic, anti-asthmatic ayurvedic herb. The study was undertaken in order to elaborate the tissue culture technique for Brahmi (Bacopa monnieri). In the present study nodal explants were taken for in vitro propagation. For sterilization, 0.01% HgCl2 was used as the sterilant. The explants gave best results when treated with the sterilant for 5 minutes. To induce bud-break, nodal segments were inoculated onto MS media supplemented with 1mg/l BAP. Basal MS medium without any plant growth hormone was used as control. Further multiplication was obtained on MS medium + BAP (1.0 mg/l) +IAA (0.5 mg/l). The shoots were rooted and best rooting was observed by IBA when incorporated in MS at different concentrations (0.1-0.3 mg/l) and 0.2 mg/l gave good results. After 30 days about 2.2 cm length was observed. Almost 70% of the rooted shoots survived during hardening."},"translated_abstract":"Bacopa monnieri, a traditional Indian medicinal plant with high commercial importance, is used as a neurotonic, immuno modulator, adaptogen transquilizing, memory and learning enhancing, cerebral activator, anti-ulcer, antispasmodic, anti-asthmatic ayurvedic herb. The study was undertaken in order to elaborate the tissue culture technique for Brahmi (Bacopa monnieri). In the present study nodal explants were taken for in vitro propagation. For sterilization, 0.01% HgCl2 was used as the sterilant. The explants gave best results when treated with the sterilant for 5 minutes. To induce bud-break, nodal segments were inoculated onto MS media supplemented with 1mg/l BAP. Basal MS medium without any plant growth hormone was used as control. Further multiplication was obtained on MS medium + BAP (1.0 mg/l) +IAA (0.5 mg/l). The shoots were rooted and best rooting was observed by IBA when incorporated in MS at different concentrations (0.1-0.3 mg/l) and 0.2 mg/l gave good results. After 30 days about 2.2 cm length was observed. Almost 70% of the rooted shoots survived during hardening.","internal_url":"https://www.academia.edu/34614828/In_vitro_propagation_of_a_medicinal_important_plant_Bacopa_monnieri_from_nodal_explants","translated_internal_url":"","created_at":"2017-09-19T23:37:39.590-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":68471456,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":30316046,"work_id":34614828,"tagging_user_id":68471456,"tagged_user_id":2800530,"co_author_invite_id":null,"email":"h***t@gmail.com","display_order":1,"name":"Himisha Dixit","title":"In vitro propagation of a medicinal important plant Bacopa monnieri from nodal explants"}],"downloadable_attachments":[{"id":54480222,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/54480222/thumbnails/1.jpg","file_name":"ijrpb_51_1_Himisha_Dixit_1-4_1.pdf","download_url":"https://www.academia.edu/attachments/54480222/download_file?st=MTczMjg1NTIyNSw4LjIyMi4yMDguMTQ2&st=MTczMjg1NTIyNSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"In_vitro_propagation_of_a_medicinal_impo.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/54480222/ijrpb_51_1_Himisha_Dixit_1-4_1-libre.pdf?1505889526=\u0026response-content-disposition=attachment%3B+filename%3DIn_vitro_propagation_of_a_medicinal_impo.pdf\u0026Expires=1732858824\u0026Signature=VJsKrKg9s-yxQV1XeYZqzvRSzhzVb2KW9Vg6CYJx7CT5oe4rVmQ83FWeeRj-zCh2BAOjpLbOSS1v2f6tfDzZjPa5LyL-mkxZPf1IKE2gmN4GoAPxfsUsCCfDTxPdZBFjx7lPqO6He7UknQF1wxwrfw555DC2KgCNLm0qeNk9inycl7uf8ilhBQ2CxPEJmtCkl2AFHzbxU~wU8XGi9cQyGWw3Xd3XvpvJS0RNn6lAf~TgVWWKK9YugsVKkswKJaFSK8th1A-WoRwdbGoui~8cahvCV2zgA9PJ~JUrskoF9-wg8X4ECO-geAZet1EDqrctvjzjo~Xb7a8~E7m7sko5wg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"In_vitro_propagation_of_a_medicinal_important_plant_Bacopa_monnieri_from_nodal_explants","translated_slug":"","page_count":4,"language":"en","content_type":"Work","owner":{"id":68471456,"first_name":"Himi","middle_initials":null,"last_name":"dixit","page_name":"Himidixit","domain_name":"independent","created_at":"2017-09-19T23:36:23.471-07:00","display_name":"Himi dixit","url":"https://independent.academia.edu/Himidixit"},"attachments":[{"id":54480222,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/54480222/thumbnails/1.jpg","file_name":"ijrpb_51_1_Himisha_Dixit_1-4_1.pdf","download_url":"https://www.academia.edu/attachments/54480222/download_file?st=MTczMjg1NTIyNSw4LjIyMi4yMDguMTQ2&st=MTczMjg1NTIyNSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"In_vitro_propagation_of_a_medicinal_impo.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/54480222/ijrpb_51_1_Himisha_Dixit_1-4_1-libre.pdf?1505889526=\u0026response-content-disposition=attachment%3B+filename%3DIn_vitro_propagation_of_a_medicinal_impo.pdf\u0026Expires=1732858825\u0026Signature=Vx2YdGtw4y29jOoSye8TsAaqURK0-J6gpp2SIHFyCepepz3BN9hiuXmlSxKWBNS51VGU7015MN-WzdE~~W7EfFOUQkrfNS6oZ1a2g9kZlmkCzVj7kXsI0sEUUAAxpCFXlGl5H3izoIKH6smuc0n4YtpRrEcD5lHXnIv5tdHXqummt7YOI3imgnOf79JGP66DwOKkT1PadznEpXxLEo~yvpmpxAgLb-pTx-m6lGdd9RsUF6ENezkd7gI1xX-qws0Vp-pOS6IS-DcRGg7XaZOJOMNF2UfDZicg~skJp9zu08NExKDdhROsbXRYtVqtuvK9M~fxWkJIzzDy6hf9cc1JAA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":68,"name":"Bioengineering","url":"https://www.academia.edu/Documents/in/Bioengineering"},{"id":140,"name":"Pharmacology","url":"https://www.academia.edu/Documents/in/Pharmacology"},{"id":656,"name":"Pharmacy","url":"https://www.academia.edu/Documents/in/Pharmacy"},{"id":1290,"name":"Immunology","url":"https://www.academia.edu/Documents/in/Immunology"},{"id":2513,"name":"Molecular Biology","url":"https://www.academia.edu/Documents/in/Molecular_Biology"},{"id":5398,"name":"Biotechnology","url":"https://www.academia.edu/Documents/in/Biotechnology"},{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="40834745"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/40834745/Antimicrobial_and_phytochemical_analysis_of_some_indigenous_plants"><img alt="Research paper thumbnail of Antimicrobial and phytochemical analysis of some indigenous plants" class="work-thumbnail" src="https://attachments.academia-assets.com/61119740/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/40834745/Antimicrobial_and_phytochemical_analysis_of_some_indigenous_plants">Antimicrobial and phytochemical analysis of some indigenous plants</a></div><div class="wp-workCard_item"><span>World Researchers Associations</span><span>, 2019</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The antimicrobial activity of the extracts of the Calotropis procera, Datura metel, Cuscuta refle...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The antimicrobial activity of the extracts of the Calotropis procera, Datura metel, Cuscuta reflexa, Pongamia pinnata and Nerium oleander has been studied against the pathogenic bacteria Staphylococcus aureus, Escherichia coli, Pseudomonas aureuginosa, Klebsiella pneumoiniae, Paenibacillus and Bacilus subtilis. Significant antibacterial activity was observed with extract of ethanol, methanol and chloroform. The ethanol extract of D. metel showed maximum antibacterial activity against the all tested bacteria except the P. aureuginosa. Aqueous extract showed no measurable antibacterial activity. Inhibition was seen as concentration dependent phenomenon. Phytochemical screening revealed the presence of alkaloids, flavanoids, glycosides, steroids, tannins, phenol and saponin.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="20f03f4380a26fdcf3e2fcbd63800d50" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{&quot;attachment_id&quot;:61119740,&quot;asset_id&quot;:40834745,&quot;asset_type&quot;:&quot;Work&quot;,&quot;button_location&quot;:&quot;profile&quot;}" href="https://www.academia.edu/attachments/61119740/download_file?st=MTczMjg1NTIyNSw4LjIyMi4yMDguMTQ2&st=MTczMjg1NTIyNSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="40834745"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="40834745"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 40834745; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=40834745]").text(description); $(".js-view-count[data-work-id=40834745]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 40834745; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='40834745']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 40834745, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "20f03f4380a26fdcf3e2fcbd63800d50" } } $('.js-work-strip[data-work-id=40834745]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":40834745,"title":"Antimicrobial and phytochemical analysis of some indigenous plants","translated_title":"","metadata":{"abstract":"The antimicrobial activity of the extracts of the Calotropis procera, Datura metel, Cuscuta reflexa, Pongamia pinnata and Nerium oleander has been studied against the pathogenic bacteria Staphylococcus aureus, Escherichia coli, Pseudomonas aureuginosa, Klebsiella pneumoiniae, Paenibacillus and Bacilus subtilis. Significant antibacterial activity was observed with extract of ethanol, methanol and chloroform. The ethanol extract of D. metel showed maximum antibacterial activity against the all tested bacteria except the P. aureuginosa. Aqueous extract showed no measurable antibacterial activity. Inhibition was seen as concentration dependent phenomenon. Phytochemical screening revealed the presence of alkaloids, flavanoids, glycosides, steroids, tannins, phenol and saponin.","publication_date":{"day":null,"month":null,"year":2019,"errors":{}},"publication_name":"World Researchers Associations"},"translated_abstract":"The antimicrobial activity of the extracts of the Calotropis procera, Datura metel, Cuscuta reflexa, Pongamia pinnata and Nerium oleander has been studied against the pathogenic bacteria Staphylococcus aureus, Escherichia coli, Pseudomonas aureuginosa, Klebsiella pneumoiniae, Paenibacillus and Bacilus subtilis. Significant antibacterial activity was observed with extract of ethanol, methanol and chloroform. The ethanol extract of D. metel showed maximum antibacterial activity against the all tested bacteria except the P. aureuginosa. Aqueous extract showed no measurable antibacterial activity. Inhibition was seen as concentration dependent phenomenon. Phytochemical screening revealed the presence of alkaloids, flavanoids, glycosides, steroids, tannins, phenol and saponin.","internal_url":"https://www.academia.edu/40834745/Antimicrobial_and_phytochemical_analysis_of_some_indigenous_plants","translated_internal_url":"","created_at":"2019-11-04T01:45:12.779-08:00","preview_url":null,"current_user_can_edit":false,"current_user_is_owner":null,"owner_id":2800530,"coauthors_can_edit":false,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":61119740,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/61119740/thumbnails/1.jpg","file_name":"Dixit_201920191104-60089-zz9uhb.pdf","download_url":"https://www.academia.edu/attachments/61119740/download_file?st=MTczMjg1NTIyNSw4LjIyMi4yMDguMTQ2&st=MTczMjg1NTIyNSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Antimicrobial_and_phytochemical_analysis.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/61119740/Dixit_201920191104-60089-zz9uhb-libre.pdf?1572862422=\u0026response-content-disposition=attachment%3B+filename%3DAntimicrobial_and_phytochemical_analysis.pdf\u0026Expires=1732858825\u0026Signature=GqjbX4ssfHpVPp9ifETVG~qEpenscClG8-3mJY9yrXjnIjqBnOPiNOhjKXsQStuV9v5gBg92gaQDPKxmRZQc087MeiVg9P1eSrF5ZJ9x5kynC2RDNGzNnbBHxwVIKh2yVmp3V7eyGmCptY6z~kShUhwO5tOqAAC1pKkhDx~X51uYFcB3oBh-2tuZaYBM4Lk7HLvyNdptXhIhHeJ3Im~CtGgEhRewYuQsxIMiOgVJGL7hQpl2Npu4HcWsQjTHwp8Q2B9sgsIOLgPceOC8PzmNW4nGeg6NVbukL393jjz~u-jJ-CnS~BtFZD72VUJiNah-ZRyFfQkGAcgpZT1vLDqfZQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Antimicrobial_and_phytochemical_analysis_of_some_indigenous_plants","translated_slug":"","page_count":8,"language":"en","content_type":"Work","owner":{"id":2800530,"first_name":"Himisha","middle_initials":null,"last_name":"Dixit","page_name":"HimishaDixit","domain_name":"independent","created_at":"2012-11-22T17:04:28.038-08:00","display_name":"Himisha Dixit","url":"https://independent.academia.edu/HimishaDixit"},"attachments":[{"id":61119740,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/61119740/thumbnails/1.jpg","file_name":"Dixit_201920191104-60089-zz9uhb.pdf","download_url":"https://www.academia.edu/attachments/61119740/download_file?st=MTczMjg1NTIyNSw4LjIyMi4yMDguMTQ2&st=MTczMjg1NTIyNSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Antimicrobial_and_phytochemical_analysis.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/61119740/Dixit_201920191104-60089-zz9uhb-libre.pdf?1572862422=\u0026response-content-disposition=attachment%3B+filename%3DAntimicrobial_and_phytochemical_analysis.pdf\u0026Expires=1732858825\u0026Signature=GqjbX4ssfHpVPp9ifETVG~qEpenscClG8-3mJY9yrXjnIjqBnOPiNOhjKXsQStuV9v5gBg92gaQDPKxmRZQc087MeiVg9P1eSrF5ZJ9x5kynC2RDNGzNnbBHxwVIKh2yVmp3V7eyGmCptY6z~kShUhwO5tOqAAC1pKkhDx~X51uYFcB3oBh-2tuZaYBM4Lk7HLvyNdptXhIhHeJ3Im~CtGgEhRewYuQsxIMiOgVJGL7hQpl2Npu4HcWsQjTHwp8Q2B9sgsIOLgPceOC8PzmNW4nGeg6NVbukL393jjz~u-jJ-CnS~BtFZD72VUJiNah-ZRyFfQkGAcgpZT1vLDqfZQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":15019,"name":"Medicinal Plants","url":"https://www.academia.edu/Documents/in/Medicinal_Plants"},{"id":67190,"name":"Phytochemical Screening of Medicinal Plants","url":"https://www.academia.edu/Documents/in/Phytochemical_Screening_of_Medicinal_Plants"},{"id":73418,"name":"Medicinal plants and natural products","url":"https://www.academia.edu/Documents/in/Medicinal_plants_and_natural_products"},{"id":111007,"name":"Antimicrobial activity","url":"https://www.academia.edu/Documents/in/Antimicrobial_activity"},{"id":1692059,"name":"Minimum inhibitory concentration","url":"https://www.academia.edu/Documents/in/Minimum_inhibitory_concentration"}],"urls":[]}, dispatcherData: dispatcherData }); 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