CINXE.COM
Synaptic glycoprotein SC2 Antibody (NBP2-49230): Novus Biologicals
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<div id="caspertesting"> <div class="container_16"> <div id="page-breadcrumb" class="grid_16"> <div class="breadcrumb"><a href="/">Home</a> » <a href="/common-name/synaptic-glycoprotein-sc2">Synaptic glycoprotein SC2</a> » <a href="/primary-antibodies/synaptic-glycoprotein-sc2">Synaptic glycoprotein SC2 Antibodies</a> » Synaptic glycoprotein SC2 Antibody</div> </div> <div class="main grid_16"> <!--&& !empty($title) to be added still--> <div class="region region-banners"> <div id="block-block-26" class="block block-block"> <div class="content"> <div class="container_12"><a href="https://www.bio-techne.com/account/novus-bio-techne/login?_ga=2.17257593.151271452<br /> 5.1724696046-473375462.1712005244" style="margin: 0 10px;" target="_blank"><img alt="Novus Biologicals products are now on bio-techne.com" src="https://images.novusbio.com/design/ready-to-buy-novus-products-login.png" style="height:120px; width:940px" /></a></div> </div> </div> </div> <h1>Synaptic glycoprotein SC2 Antibody</h1> </div> <div class="clear"></div> <div> <div class="region region-content"> <div id="block-et-messages-et-messages-block" class="block block-et-messages"> <div class="content"> <span></span> </div> </div> <div id="block-system-main" class="block block-system"> <div class="content"> <div class="grid_16"><div class="ds_info float_left""><div class="ds_info float_left"><a href="/reviews/NBP2-49230"><div class="dsSprite_submitreview"></div></a> <a href="/reviews/NBP2-49230" class="bottom4"><span class="ctr">Submit a Review</span></a></div></div></div><div class="modalWrapper" style="display:none;"> <h2>Species Glossary</h2> <hr class="customhr"/> <img src="//images.novusbio.com/design/bunny.gif" alt="Species Glossary Rabbit" style="float: right;"> <table class="modalTable"> <tr> <td>At</td> <td>=</td> <td>A. thaliana</td> <td>SyHa</td> <td>=</td> <td>Golden Syrian Hamster</td> </tr> <tr> <td>All</td> <td>=</td> <td>All Species</td> <td>Ha</td> <td>=</td> <td>Hamster</td> </tr> <tr> <td>An</td> <td>=</td> <td>Animal</td> <td>Hu</td> <td>=</td> <td>Human</td> </tr> <tr> <td>ArHa</td> <td>=</td> <td>Armenian Hamster</td> <td>I</td> <td>=</td> <td>Insect</td> </tr> <tr> <td>Av</td> <td>=</td> <td>Avian</td> <td>Ll</td> <td>=</td> <td>Llama</td> </tr> <tr> <td>Bb</td> <td>=</td> <td>Baboon</td> <td>Ma</td> <td>=</td> <td>Mammal</td> </tr> <tr> <td>Ba</td> <td>=</td> <td>Bacteria</td> <td>Mk</td> <td>=</td> <td>Monkey</td> </tr> <tr> <td>Bv</td> <td>=</td> <td>Bovine</td> <td>Mu</td> <td>=</td> <td>Mouse</td> </tr> <tr> <td>Ca</td> <td>=</td> <td>Canine</td> <td>Multi</td> <td>=</td> <td>Multi-species</td> </tr> <tr> <td>Ce</td> <td>=</td> <td>C. Elegans</td> <td>NA</td> <td>=</td> <td>Non-species specific</td> </tr> <tr> <td>Ch</td> <td>=</td> <td>Chicken</td> <td>Or</td> <td>=</td> <td>Orangutan</td> </tr> <tr> <td>ChHa</td> <td>=</td> <td>Chinese Hamster</td> <td>Pl</td> <td>=</td> <td>Plant</td> </tr> <tr> <td>Cm</td> <td>=</td> <td>Cynomolgus Monkey</td> <td>Pm</td> <td>=</td> <td>Primate</td> </tr> <tr> <td>Do</td> <td>=</td> <td>Donkey</td> <td>Po</td> <td>=</td> <td>Porcine</td> </tr> <tr> <td>Dr</td> <td>=</td> <td>Drosophilia</td> <td>Rb</td> <td>=</td> <td>Rabbit</td> </tr> <tr> <td>Ec</td> <td>=</td> <td>E. Coli</td> <td>Rt</td> <td>=</td> <td>Rat</td> </tr> <tr> <td>Eq</td> <td>=</td> <td>Equine</td> <td>RM</td> <td>=</td> <td>Rhesus Macaque</td> </tr> <tr> <td>Fe</td> <td>=</td> <td>Feline</td> <td>Sh</td> <td>=</td> <td>Sheep</td> </tr> <tr> <td>Ft</td> <td>=</td> <td>Ferret</td> <td>Vb</td> <td>=</td> <td>Vertebrate</td> </tr> <tr> <td>Fi</td> <td>=</td> <td>Fish</td> <td>Vi</td> <td>=</td> <td>Virus</td> </tr> <tr> <td>Fu</td> <td>=</td> <td>Fungi</td> <td>Xp</td> <td>=</td> <td>Xenopus</td> </tr> <tr> <td>Ge</td> <td>=</td> <td>Gerbil</td> <td>Ye</td> <td>=</td> <td>Yeast</td> </tr> <tr> <td>GP</td> <td>=</td> <td>Guinea Pig</td> <td>Ze</td> <td>=</td> <td>Zebrafish</td> </tr> <tr> <td>Gt</td> <td>=</td> <td>Goat</td> </tr> </table> <div style="text-align: center;margin-left:25px;margin-right:25px;"> <p>Note: Mouseover a species abbreviation on the product page to display the fullname.</p> </div> </div> </div><div class="clear"></div><div class="grid_10 pos_relative"><div class="ds_blue_border equaltop"><div class="imagesleft equaltop"><h2 class="blueHead">Images</h2> <div class="midBlue"> </div><div class="images"><div id="ss_prev_top" class="cycle-pager"><div class="pager_left_on"></div></div><div class="cycle-slideshow slideshows" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-top" data-cycle-prev="#ss_prev_top" data-cycle-next="#ss_next_top" data-cycle-caption="#image-caption" data-cycle-caption-template="Image {{slideNum}} of {{slideCount}}" data-cycle-slides=".big_thumb_container" data-cycle-carousel-visible="3" id="images_cycle" > <div class="sub_image_wrapper"><div id="cycle-circle-pager-top" class="cycle-circle-pager"></div><div id="image-caption"></div> <a class="enlargeTop">(<div class="dsSprite_magGlass"></div> Enlarge)</a></div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize/Synaptic-glycoprotein-SC2-Antibody-Western-Blot-NBP2-49230-img0005.jpg" class="top_images" rel="gallery" title="Western Blot: Synaptic glycoprotein SC2 Antibody [NBP2-49230] - Analysis in control (vector only transfected HEK293T lysate) and TECR over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells)." > <img src="//images.novusbio.com/images/Synaptic-glycoprotein-SC2-Antibody-Western-Blot-NBP2-49230-img0005.jpg" class="big_thumb" alt="Western Blot: Synaptic glycoprotein SC2 Antibody [NBP2-49230] - Analysis in control (vector only transfected HEK293T lysate) and TECR over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells)." title="Western Blot: Synaptic glycoprotein SC2 Antibody [NBP2-49230] - Analysis in control (vector only transfected HEK293T lysate) and TECR over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells)." /> </a> </div> <div class="alt-caption padding_left5" >Western Blot: Synaptic glycoprotein SC2 Antibody [NBP2-49230] - Analysis in control (vector only transfected HEK293T lysate) and TECR over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in<a class="openGallery" href="#" data-picnum="0"> ...read more</a></div> </div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize/Synaptic-glycoprotein-SC2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-49230-img0004.jpg" class="top_images" rel="gallery" title="Immunocytochemistry/Immunofluorescence: Synaptic glycoprotein SC2 Antibody [NBP2-49230] - Immunofluorescent staining of human cell line U-251 MG shows localization to endoplasmic reticulum." > <img src="//images.novusbio.com/images/Synaptic-glycoprotein-SC2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-49230-img0004.jpg" loading="lazy" style="display:none;" class="big_thumb" alt="Immunocytochemistry/Immunofluorescence: Synaptic glycoprotein SC2 Antibody [NBP2-49230] - Immunofluorescent staining of human cell line U-251 MG shows localization to endoplasmic reticulum." title="Immunocytochemistry/Immunofluorescence: Synaptic glycoprotein SC2 Antibody [NBP2-49230] - Immunofluorescent staining of human cell line U-251 MG shows localization to endoplasmic reticulum." /> </a> </div> <div class="alt-caption padding_left5" >Immunocytochemistry/ Immunofluorescence: Synaptic glycoprotein SC2 Antibody [NBP2-49230] - Immunofluorescent staining of human cell line U-251 MG shows localization to endoplasmic reticulum.</div> </div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize/Synaptic-glycoprotein-SC2-Antibody-Immunohistochemistry-Paraffin-NBP2-49230-img0002.jpg" class="top_images" rel="gallery" title="Immunohistochemistry-Paraffin: Synaptic glycoprotein SC2 Antibody [NBP2-49230] - Staining of human rectum shows moderate cytoplasmic positivity in glandular cells." > <img src="//images.novusbio.com/images/Synaptic-glycoprotein-SC2-Antibody-Immunohistochemistry-Paraffin-NBP2-49230-img0002.jpg" loading="lazy" style="display:none;" class="big_thumb" alt="Immunohistochemistry-Paraffin: Synaptic glycoprotein SC2 Antibody [NBP2-49230] - Staining of human rectum shows moderate cytoplasmic positivity in glandular cells." title="Immunohistochemistry-Paraffin: Synaptic glycoprotein SC2 Antibody [NBP2-49230] - Staining of human rectum shows moderate cytoplasmic positivity in glandular cells." /> </a> </div> <div class="alt-caption padding_left5" >Immunohistochemistry-Paraffin: Synaptic glycoprotein SC2 Antibody [NBP2-49230] - Staining of human rectum shows moderate cytoplasmic positivity in glandular cells.</div> </div></div><div id="ss_next_top" class="cycle-pager"><div class="pager_right_on"></div></div></div></div><div class="productsright pos_relative equaltop"><h2 class="blueHead">Product Details</h2> <div class="midBlue">Summary</div><table class="ds_list"><tr class="lined"> <td><strong>Reactivity</strong></td> <td><span title='Human'>Hu</span><span class="myModal">Species Glossary</span></td> </tr><tr class="lined"> <td><strong>Applications</strong></td> <td><span title='Western Blot'>WB</span>, <span title='Immunocytochemistry/ Immunofluorescence'>ICC/IF</span>, <span title='Immunohistochemistry'>IHC</span></td> </tr><tr class="lined"> <td><strong>Clonality</strong></td> <td><div>Polyclonal</div></td> </tr><tr class="lined"> <td><strong>Host</strong></td> <td><div>Rabbit</div></td> </tr><tr class="lined"> <td><strong>Conjugate</strong></td> <td><div>Unconjugated</div></td> </tr></table></div> </div></div><div class="grid_6"><div></div> <div class="ds_blue_border equaltop"> <h2 class="blueHead_nopadding">Order Details</h2><ul class="ds_details"><li class="add-to-cart" data-type="full" data-prodname="" data-catnum="NBP2-49230" data-catnums="NBP2-49230" data-disabled=''><noscript><form autocomplete="off" action="/products/synaptic-glycoprotein-sc2-antibody_nbp2-49230" method="post" id="et-add-multiple-to-cart-form" accept-charset="UTF-8"><div><table class="sticky-enabled"> <thead><tr><th>Size</th><th>Price</th> </tr></thead> <tbody> <tr class="odd"><td><div class="atc_size" title="catalog # NBP2-49230">0.1 ml</div><div data-msg='' class="atc_catnum">NBP2-49230</div></td><td><div style="font-size:11px;"><a class="jump_distributor" href="#jump_distributor">Contact Your<br />Distributor</a></div></td> </tr> <tr class="even"><td colspan="3" class="stock_row">7-9 Business Days</td> </tr> </tbody> </table> <input type="hidden" name="form_build_id" value="form-aaP-2rRmsmd_8kmpljmknBiyO-hohnMxpMfC88lv7FE" /> <input type="hidden" name="form_id" value="et_add_multiple_to_cart_form" /> </div></form></noscript></li></ul><div id="shipping" class="hidden"></td></tr></div><div class="conj_form_buttons"></div><div id="shipping_statement"><span id="shipping_rate"></span></div><div class="shipping_obt_transfer"><span>Novus Biologicals is part of Bio-Techne</span><br><br><a href="https://www.bio-techne.com/p/antibodies/synaptic-glycoprotein-sc2-antibody_nbp2-49230" rel="follow" class="btn btn-primary">Shop this product on bio-techne.com</a></div><br><div class="learn_more"> <a href="/guarantee"> <div class="sprite_noImage" title="Novus Biologicals, part of Bio-Techne, offers 100% Guarantee"></div> <div class="learn_more_text">Novus Biologicals, part of Bio-Techne, offers 100% Guarantee</div></a> </div><span id="conj_form" class="hidden"><tr><td colspan="4"></td></tr></span> </div> </div><div class="clear"></div><div class="grid_10"><div><div id="ds_tabs" style="display:none;"> <div class="ds_tab_active" id="dsTabs_datasheet" data-anchor_desc="datasheet">Datasheet</div> <div class="ds_tab" id="dsTabs_reviews-publications" data-anchor_desc="reviews-publications">Reviews & Publications</div> <div class="ds_tab" id="dsTabs_protocols-faqs" data-anchor_desc="protocols-faqs">Protocols & FAQs</div> <div class="ds_tab" id="dsTabs_supportresearch" data-anchor_desc="supportresearch">Support & Research</div> </div><div class="ds_tab_content equalbottom" id="content_datasheet" > <a id="datasheet"></a><h2 class=greyHead2>Synaptic glycoprotein SC2 Antibody Summary</h2> <div class="ds_info"> <a id="tab1details"></a> <table class="ds_list wide" style="table-layout:fixed;"> <tr class="lined"> <td><strong>Immunogen</strong></td> <td><div>This antibody was developed against a recombinant protein corresponding to amino acids: MKHYEVEILDAKTREKLCFLDKVEPHATIAEIKNLF</div></td> </tr><tr class="lined"> <td><strong>Isotype</strong></td> <td><div>IgG</div></td> </tr><tr class="lined"> <td><strong>Clonality</strong></td> <td><div>Polyclonal</div></td> </tr><tr class="lined"> <td><strong>Host</strong></td> <td><div>Rabbit</div></td> </tr><tr class="lined"> <td><strong>Gene</strong></td> <td><div>TECR</div></td> </tr><tr class="lined"> <td><strong>Purity</strong></td> <td><div>Immunogen affinity purified</div></td> </tr><tr class="lined"> <td><strong>Innovator's Reward</strong></td> <td><div>Test in a species/application not listed above to receive a full credit towards a future purchase.<br/><div class='text_right'><a class='ds_submit_blue ds_regularPad' href='/support/innovators-reward.html'>Learn about the Innovator's Reward</a></div></div></td> </tr> </table> </div> <h2 class="greyHead2">Applications/Dilutions</h2> <div class="ds_info"> <a id="tab1appsdiluts"></a> <table class="ds_list wide"> <tr class="lined"> <td><strong>Dilutions</strong></td> <td> <ul class="ds_noDotList"><li>Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml</li><li>Immunohistochemistry 1:200 - 1:500</li><li>Immunohistochemistry-Paraffin 1:200 - 1:500</li><li>Western Blot 0.04-0.4 ug/ml</li> </ul> </td> </tr><tr class="lined"> <td><strong>Application Notes</strong></td> <td><div>For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.</div></td> </tr> <tr class="lined"> <td><strong>Control Peptide</strong></td> <td> <table class="wide ds_altTable"><tr> <td style="width:80%"><a href="/synaptic-glycoprotein-sc2-recombinant-protein-antigen_nbp2-49230pep">Synaptic glycoprotein SC2 Recombinant Protein Antigen (NBP2-49230PEP)</a></td> <td class="text_right"> <div class="add-to-cart" data-type="anc_btnonly" data-catbase="NBP2-49230PEP" data-return="synaptic-glycoprotein-sc2-antibody_nbp2-49230" data-produrl="synaptic-glycoprotein-sc2-antibody_nbp2-49230" data-catnums="NBP2-49230PEP" ></div> </td> </tr></table> </td> </tr> </table> </div><h2 class="greyHead2">Reactivity Notes</h2> <div> <a id="tab1speciesreactivity"></a> <div class="ds_info"> Mouse (89%), Rat (89%)</div> </div> <h2 class="greyHead2">Packaging, Storage & Formulations</h2><div class="ds_info"><table class="ds_list"><tr class="lined"> <td><strong>Storage</strong></td> <td><div>Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.</div></td> </tr><tr class="lined"> <td><strong>Buffer</strong></td> <td><div>PBS (pH 7.2) and 40% Glycerol</div></td> </tr><tr class="lined"> <td><strong>Preservative</strong></td> <td><div>0.02% Sodium Azide</div></td> </tr><tr class="lined"> <td><strong>Purity</strong></td> <td><div>Immunogen affinity purified</div></td> </tr></table></div><h2 class="greyHead2">Alternate Names for Synaptic glycoprotein SC2 Antibody</h2> <div class="ds_info"> <a id="tab1altnames"></a> <div class="information-list"> <ul><li>EC 1.3.1</li><li>EC 1.3.1.38</li><li>glycoprotein, synaptic 2</li><li>GPSN2</li><li>Synaptic glycoprotein SC2</li><li>TERSC2</li><li>trans-2,3-enoyl-CoA reductase</li></ul></div> </div> <h2 class="greyHead2">Background</h2> <div class="ds_info"> <a id="tab1background"></a> <div>Microsomal long and very long chain fatty acid elongation uses malonyl-CoA as the 2-carbon donor and consists of 4sequential reactions. TER catalyzes the final step, reducing trans-2,3-enoyl-CoA to saturated acyl-CoA (Moon andHorton, 2003 (PubMed 12482854)).(supplied by OMIM)</div> </div> <h2 class="greyHead2">Limitations</h2> <div class="ds_info"> <a id="tab1background"></a> <div>This product is for research use only and is not approved for use in humans or in clinical diagnosis. 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Antibody concentration 5 ug/ml." class="cust_viewed_2" > <img loading="lazy" src="https://images.novusbio.com/images/Claudin-1-Antibody-1C5-D9-Immunohistochemistry-Paraffin-H00009076-M01-img0011.jpg" alt="Immunohistochemistry-Paraffin Claudin-1 Antibody (1C5-D9)" class="big_thumb" /> </a> </div></div><div id="ss_next_cav2" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-2" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/claudin-1-antibody-1c5-d9_h00009076-m01#ReviewsSection"> <div class="sprite_5Stars"></div> (2 Reviews</a>) </div> <div class="tiny"> <a href="/products/claudin-1-antibody-1c5-d9_h00009076-m01#PublicationSection"> <div class="sprite_pubBook"></div> (26 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu, Pm, Mu</div><div class="tiny"><strong>Applications:</strong> ELISA, Flow, ICC/IF, IHC, IHC-P, IP, WB</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="210-TA"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/recombinant-human-tnf-alpha-protein_210-ta" style="overflow: hidden; width: 280px;" title="TNF-alpha [Unconjugated]">TNF-alpha<br> [Unconjugated]</a> <!--<div class="purchase_cart_wrapper"> <div class="purchase_cart btnAdd" data-type="anc_btnonly" data-catbase="210-TA" data-return="synaptic-glycoprotein-sc2-antibody_nbp2-49230#datasheet" data-produrl="synaptic-glycoprotein-sc2-antibody_nbp2-49230" data-catnums="210-TA" data-baseurl="https://www.novusbio.com" data-productid="447011"></div>--> <!-- <a href="/commerce/add-to-cart/447011"><div class="purchase_cart"></div></a> --> <!--</div>--> </div><div class="sub-header">210-TA</div> <div class="images_customers"><div id="ss_prev_cav3" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-3" data-cycle-prev="#ss_prev_cav3" data-cycle-next="#ss_next_cav3" data-cycle-slides="div.big_thumb_cust_container" data-index=3 ><div class="big_thumb_cust_container"> <a href="/products/recombinant-human-tnf-alpha-protein_210-ta" title="1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa." class="cust_viewed_3" > <img loading="lazy" src="https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg" alt="SDS-PAGE TNF-alpha [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/recombinant-human-tnf-alpha-protein_210-ta" title="Recombinant Human TNF-alpha (Catalog # 210‑TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 25‑100 pg/mL." class="cust_viewed_3" > <img loading="lazy" src="https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg" alt="Bioactivity TNF-alpha [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/recombinant-human-tnf-alpha-protein_210-ta" title="Recombinant Human TNF-alpha (Catalog # 210-TA) has a molecular weight (MW) of 53.1 kDa as analyzed by SEC-MALS, suggesting that this protein is a homotrimer. MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation)." class="cust_viewed_3" > <img loading="lazy" src="https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg" alt="SEC-MALS TNF-alpha [Unconjugated]" class="big_thumb" /> </a> </div></div><div id="ss_next_cav3" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-3" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/recombinant-human-tnf-alpha-protein_210-ta#ReviewsSection"> <div class="sprite_5Stars"></div> (3 Reviews</a>) </div> <div class="tiny"> <a href="/products/recombinant-human-tnf-alpha-protein_210-ta#PublicationSection"> <div class="sprite_pubBook"></div> (811 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu</div><div class="tiny"><strong>Applications:</strong> BA</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NBP1-87402"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/occludin-antibody_nbp1-87402" style="overflow: hidden; width: 280px;" title="Occludin Antibody">Occludin Antibody</a> <!--<div class="purchase_cart_wrapper"> <div class="purchase_cart btnAdd" data-type="anc_btnonly" data-catbase="NBP1-87402" data-return="synaptic-glycoprotein-sc2-antibody_nbp2-49230#datasheet" data-produrl="synaptic-glycoprotein-sc2-antibody_nbp2-49230" data-catnums="NBP1-87402" data-baseurl="https://www.novusbio.com" data-productid="286196"></div>--> <!-- <a href="/commerce/add-to-cart/286196"><div class="purchase_cart"></div></a> --> <!--</div>--> </div><div class="sub-header">NBP1-87402</div> <div class="images_customers"><div id="ss_prev_cav4" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-4" data-cycle-prev="#ss_prev_cav4" data-cycle-next="#ss_next_cav4" data-cycle-slides="div.big_thumb_cust_container" data-index=4 ><div class="big_thumb_cust_container"> <a href="/products/occludin-antibody_nbp1-87402" title="Western Blot: Occludin Antibody [NBP1-87402] - Lane 1: untreated vascular endothelial cells Lane 2: vascular endothelial cells + control siRNA. Primary antibody was diluted 1:1000. WB image submitted by a verified customer review." class="cust_viewed_4" > <img loading="lazy" src="https://images.novusbio.com/images/Occludin-Antibody-Western-Blot-NBP1-87402-img0009.jpg" alt="Western Blot Occludin Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/occludin-antibody_nbp1-87402" title="Immunohistochemistry-Paraffin: Occludin Antibody [NBP1-87402] - Staining of human breast cancer shows moderate membranous positivity in tumor cells." class="cust_viewed_4" > <img loading="lazy" src="https://images.novusbio.com/images/Occludin-Antibody-Immunohistochemistry-Paraffin-NBP1-87402-img0016.jpg" alt="Immunohistochemistry-Paraffin Occludin Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/occludin-antibody_nbp1-87402" title="Immunohistochemistry-Paraffin: Occludin Antibody [NBP1-87402] - Staining of human prostate cancer shows moderate membranous positivity in tumor cells." class="cust_viewed_4" > <img loading="lazy" src="https://images.novusbio.com/images/Occludin-Antibody-Immunohistochemistry-Paraffin-NBP1-87402-img0017.jpg" alt="Immunohistochemistry-Paraffin Occludin Antibody" class="big_thumb" /> </a> </div></div><div id="ss_next_cav4" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-4" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/occludin-antibody_nbp1-87402#ReviewsSection"> <div class="sprite_4Stars"></div> (4 Reviews</a>) </div> <div class="tiny"> <a href="/products/occludin-antibody_nbp1-87402#PublicationSection"> <div class="sprite_pubBook"></div> (23 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu, Mu, Rt</div><div class="tiny"><strong>Applications:</strong> ELISA, IHC, IHC-P, KD, WB</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="MAB1455"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/albumin-antibody-188835_mab1455" style="overflow: hidden; width: 280px;" title="Albumin Antibody (188835) [Unconjugated] - Serum">Albumin Antibody<br> (188835) [Unconjugate ...</a> <!--<div class="purchase_cart_wrapper"> <div class="purchase_cart btnAdd" data-type="anc_btnonly" data-catbase="MAB1455" data-return="synaptic-glycoprotein-sc2-antibody_nbp2-49230#datasheet" data-produrl="synaptic-glycoprotein-sc2-antibody_nbp2-49230" data-catnums="MAB1455" data-baseurl="https://www.novusbio.com" data-productid="419288"></div>--> <!-- <a href="/commerce/add-to-cart/419288"><div class="purchase_cart"></div></a> --> <!--</div>--> </div><div class="sub-header">MAB1455</div> <div class="images_customers"><div id="ss_prev_cav5" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-5" data-cycle-prev="#ss_prev_cav5" data-cycle-next="#ss_next_cav5" data-cycle-slides="div.big_thumb_cust_container" data-index=5 ><div class="big_thumb_cust_container"> <a href="/products/albumin-antibody-188835_mab1455" title=" Simple Western lane view shows lysates of human serum, loaded at 1:25000. A specific band was detected for Albumin at approximately 64&amp;nbsp;kDa (as indicated) using 1&amp;nbsp;µg/mL of Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB1455) . This experiment was conducted under reducing&amp;nbsp;conditions and using the 12-230 kDa separation system. " class="cust_viewed_5" > <img loading="lazy" src="https://images.novusbio.com/images2/Albumin_MAB1455_Simple_Western_21888.jpg" alt="Simple Western Albumin Antibody (188835) [Unconjugated] - Serum" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/albumin-antibody-188835_mab1455" title="Albumin was detected in immersion fixed BG01V human embryonic stem cells differentiated to hepatocytes using Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB1455) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights&amp;#8482; 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Cells were co-stained using Sheep Anti-Human CEBP alpha (Catalog # AF7094) and NorthernLights&amp;#8482; 493-conjugated Anti-Sheep IgG Secondary Antibody (green, Catalog # NL012). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="cust_viewed_5" > <img loading="lazy" src="https://images.novusbio.com/images2/Albumin_MAB1455_Immunocytochemistry__Immunofluorescence_17777.jpg" alt="Immunocytochemistry Albumin Antibody (188835) [Unconjugated] - Serum" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/albumin-antibody-188835_mab1455" title=" Albumin was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB1455) at 0.1&amp;nbsp;µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte&amp;#8482; HRP Polymer Antibody (Catalog&amp;nbsp;# VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="cust_viewed_5" > <img loading="lazy" src="https://images.novusbio.com/images2/Albumin_MAB1455_Immunohistochemistry_23317.jpg" alt="Immunohistochemistry Albumin Antibody (188835) [Unconjugated] - Serum" class="big_thumb" /> </a> </div></div><div id="ss_next_cav5" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-5" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/albumin-antibody-188835_mab1455#ReviewsSection"> <div class="sprite_4Stars"></div> (3 Reviews</a>) </div> <div class="tiny"> <a href="/products/albumin-antibody-188835_mab1455#PublicationSection"> <div class="sprite_pubBook"></div> (54 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu</div><div class="tiny"><strong>Applications:</strong> CyTOF-ready, Dual ISH-IHC, ICC, IHC, ICFlow, Simple Western, WB</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="DVE00"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/human-vegf-quantikine-elisa-kit_dve00" style="overflow: hidden; width: 280px;" title="VEGF [HRP]">VEGF [HRP]</a> <!--<div class="purchase_cart_wrapper"> <div class="purchase_cart btnAdd" data-type="anc_btnonly" data-catbase="DVE00" data-return="synaptic-glycoprotein-sc2-antibody_nbp2-49230#datasheet" data-produrl="synaptic-glycoprotein-sc2-antibody_nbp2-49230" data-catnums="DVE00" data-baseurl="https://www.novusbio.com" data-productid="445814"></div>--> <!-- <a href="/commerce/add-to-cart/445814"><div class="purchase_cart"></div></a> --> <!--</div>--> </div><div class="sub-header">DVE00</div> <div class="images_customers"><div id="ss_prev_cav6" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-6" data-cycle-prev="#ss_prev_cav6" data-cycle-next="#ss_next_cav6" data-cycle-slides="div.big_thumb_cust_container" data-index=6 ><div class="big_thumb_cust_container"> <a href="/products/human-vegf-quantikine-elisa-kit_dve00" title="" class="cust_viewed_6" > <img loading="lazy" src="https://images.novusbio.com/images2/DATA_VEGF_DVE00_ELISA_871.jpg" alt="N/A VEGF [HRP]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/human-vegf-quantikine-elisa-kit_dve00" title="" class="cust_viewed_6" > <img loading="lazy" src="https://images.novusbio.com/images2/DATA_VEGF_DVE00_ELISA_872.jpg" alt="N/A VEGF [HRP]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/human-vegf-quantikine-elisa-kit_dve00" title="" class="cust_viewed_6" > <img loading="lazy" src="https://images.novusbio.com/images2/VEGF_DVE00_ELISA_208.jpg" alt="N/A VEGF [HRP]" class="big_thumb" /> </a> </div></div><div id="ss_next_cav6" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-6" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/human-vegf-quantikine-elisa-kit_dve00#PublicationSection"> <div class="sprite_pubBook"></div> (683 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu</div><div class="tiny"><strong>Applications:</strong> ELISA</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NBP2-22203"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/erk1-antibody-1e5_nbp2-22203" style="overflow: hidden; width: 280px;" title="ERK1 Antibody (1E5) - BSA Free">ERK1 Antibody<br> (1E5) - BSA Free</a> <!--<div class="purchase_cart_wrapper"> <div class="purchase_cart btnAdd" data-type="anc_btnonly" data-catbase="NBP2-22203" data-return="synaptic-glycoprotein-sc2-antibody_nbp2-49230#datasheet" data-produrl="synaptic-glycoprotein-sc2-antibody_nbp2-49230" data-catnums="NBP2-22203" data-baseurl="https://www.novusbio.com" data-productid="334033"></div>--> <!-- <a href="/commerce/add-to-cart/334033"><div class="purchase_cart"></div></a> --> <!--</div>--> </div><div class="sub-header">NBP2-22203</div> <div class="images_customers"><div id="ss_prev_cav7" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-7" data-cycle-prev="#ss_prev_cav7" data-cycle-next="#ss_next_cav7" data-cycle-slides="div.big_thumb_cust_container" data-index=7 ><div class="big_thumb_cust_container"> <a href="/products/erk1-antibody-1e5_nbp2-22203" title="Flow Cytometry: ERK1 Antibody (1E5) [NBP2-22203] - Flow cytometric analysis of Hela cells using ERK1 mouse mAb (blue) and negative control (red)." class="cust_viewed_7" > <img loading="lazy" src="https://images.novusbio.com/images/ERK1-Antibody-1E5-Flow-Cytometry-NBP2-22203-img0001.jpg" alt="Flow Cytometry ERK1 Antibody (1E5) - BSA Free" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/erk1-antibody-1e5_nbp2-22203" title="Immunocytochemistry/Immunofluorescence: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of NIH/3T3 cells using ERK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin." class="cust_viewed_7" > <img loading="lazy" src="https://images.novusbio.com/images/ERK1-Antibody-1E5-Immunocytochemistry-Immunofluorescence-NBP2-22203-img0002.jpg" alt="Immunocytochemistry/ Immunofluorescence ERK1 Antibody (1E5) - BSA Free" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/erk1-antibody-1e5_nbp2-22203" title="Immunohistochemistry-Paraffin: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of paraffin-embedded bladder cancer tissues using ERK1 mouse mAb with DAB staining." class="cust_viewed_7" > <img loading="lazy" src="https://images.novusbio.com/images/ERK1-Antibody-1E5-Immunohistochemistry-Paraffin-NBP2-22203-img0003.jpg" alt="Immunohistochemistry-Paraffin ERK1 Antibody (1E5) - BSA Free" class="big_thumb" /> </a> </div></div><div id="ss_next_cav7" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-7" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/erk1-antibody-1e5_nbp2-22203#PublicationSection"> <div class="sprite_pubBook"></div> (1 Publication</a>) </div><div class="tiny"><strong>Species:</strong> Hu, Pm, Mu, Rt</div><div class="tiny"><strong>Applications:</strong> ELISA, Flow, ICC/IF, IHC, IHC-P, WB</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="AF1230"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/erk2-antibody_af1230" style="overflow: hidden; width: 280px;" title="ERK2 Antibody [Unconjugated]">ERK2 Antibody<br> [Unconjugated]</a> <!--<div class="purchase_cart_wrapper"> <div class="purchase_cart btnAdd" data-type="anc_btnonly" data-catbase="AF1230" data-return="synaptic-glycoprotein-sc2-antibody_nbp2-49230#datasheet" data-produrl="synaptic-glycoprotein-sc2-antibody_nbp2-49230" data-catnums="AF1230" data-baseurl="https://www.novusbio.com" data-productid="413272"></div>--> <!-- <a href="/commerce/add-to-cart/413272"><div class="purchase_cart"></div></a> --> <!--</div>--> </div><div class="sub-header">AF1230</div> <div class="images_customers"><div id="ss_prev_cav8" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-8" data-cycle-prev="#ss_prev_cav8" data-cycle-next="#ss_next_cav8" data-cycle-slides="div.big_thumb_cust_container" data-index=8 ><div class="big_thumb_cust_container"> <a href="/products/erk2-antibody_af1230" title="Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, U937 human histiocytic lymphoma cell line, PC-12 rat adrenal pheochromocytoma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse/Rat ERK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1230) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for ERK2 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="cust_viewed_8" > <img loading="lazy" src="https://images.novusbio.com/images2/ERK2_AF1230_Western_Blot_5097.jpg" alt="Western Blot ERK2 Antibody [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/erk2-antibody_af1230" title=" ERK2 was detected in immersion fixed paraffin-embedded sections of human breast using Rabbit Anti-Human/Mouse/Rat ERK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1230) at 3&amp;nbsp;µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte&amp;#8482; HRP Polymer Antibody (Catalog&amp;nbsp;# VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents. " class="cust_viewed_8" > <img loading="lazy" src="https://images.novusbio.com/images2/ERK2_AF1230_Immunohistochemistry_20696.jpg" alt="Immunohistochemistry ERK2 Antibody [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/erk2-antibody_af1230" title="Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and ERK2 knockout HeLa cell line (KO). PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse/Rat ERK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1230) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for ERK2 at approximately 42 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="cust_viewed_8" > <img loading="lazy" src="https://images.novusbio.com/images2/ERK2_AF1230_Knockout_Validated_22864.jpg" alt="Knockout Validated ERK2 Antibody [Unconjugated]" class="big_thumb" /> </a> </div></div><div id="ss_next_cav8" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-8" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/erk2-antibody_af1230#PublicationSection"> <div class="sprite_pubBook"></div> (3 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu, Mu, Rt</div><div class="tiny"><strong>Applications:</strong> IHC, KO, WB</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="AF2836"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/sparc-like-1-sparcl1-antibody_af2836" style="overflow: hidden; width: 280px;" title="SPARC-like 1/SPARCL1 Antibody">SPARC-like<br> 1/SPARCL1 Antibody</a> <!--<div class="purchase_cart_wrapper"> <div class="purchase_cart btnAdd" data-type="anc_btnonly" data-catbase="AF2836" data-return="synaptic-glycoprotein-sc2-antibody_nbp2-49230#datasheet" data-produrl="synaptic-glycoprotein-sc2-antibody_nbp2-49230" data-catnums="AF2836" data-baseurl="https://www.novusbio.com" data-productid="410272"></div>--> <!-- <a href="/commerce/add-to-cart/410272"><div class="purchase_cart"></div></a> --> <!--</div>--> </div><div class="sub-header">AF2836</div> <div class="images_customers"><div id="ss_prev_cav9" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-9" data-cycle-prev="#ss_prev_cav9" data-cycle-next="#ss_next_cav9" data-cycle-slides="div.big_thumb_cust_container" data-index=9 ><div class="big_thumb_cust_container"> <a href="/products/sparc-like-1-sparcl1-antibody_af2836" title="Hevin expression by astrocytes is developmentally regulated in the cortex.(A) Representative Western blots showing the developmental timeline for hevin expression in mouse cortex and hippocampus (tubulin was used as a loading control). (B) Quantification of Western blot analysis of hevin expression shows high expression between P15–P25. Data is presented as fold change compared to P1 levels (n = 3 animals per age; p &lt; 0.05; one-way ANOVA with Dunnett's post hoc test). (C) Schematic diagram of a coronal slice through mouse brain shows the synaptic zone of primary visual cortex (V1) where EM, IHC and Golgi-cox staining analyses were performed. Layer II/III neurons of the visual cortex heavily project their dendrites to this region (D) IHC staining reveals that hevin expression (green) overlaps with all astrocytes (left, arrow) and a small subset of neurons (middle, asterisk) in V1, with no overlap seen with microglia (right, arrowhead). Cell-specific markers in red: Aldh1L1-EGFP for astrocytes, NeuN for neurons, Iba1 for microglia. Scale bar, 50 µm. (E) The rarely occurring GFAP+ astrocytes (red) in healthy visual cortex also express hevin (green). Scale bar, 10 µm.DOI:https://dx.doi.org/10.7554/eLife.04047.003 Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25517933), licensed under a CC-BY license. Not internally tested by R&amp;D Systems." class="cust_viewed_9" > <img loading="lazy" src="https://images.novusbio.com/images/af2836_mouse-sparc-like-1-sparcl1-affinity-purified-polyclonal-ab-4120241240513.jpg" alt="Immunocytochemistry/ Immunofluorescence SPARC-like 1/SPARCL1 Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/sparc-like-1-sparcl1-antibody_af2836" title="Hevin expression by astrocytes is developmentally regulated in the cortex.(A) Representative Western blots showing the developmental timeline for hevin expression in mouse cortex and hippocampus (tubulin was used as a loading control). (B) Quantification of Western blot analysis of hevin expression shows high expression between P15–P25. Data is presented as fold change compared to P1 levels (n = 3 animals per age; p &lt; 0.05; one-way ANOVA with Dunnett's post hoc test). (C) Schematic diagram of a coronal slice through mouse brain shows the synaptic zone of primary visual cortex (V1) where EM, IHC and Golgi-cox staining analyses were performed. Layer II/III neurons of the visual cortex heavily project their dendrites to this region (D) IHC staining reveals that hevin expression (green) overlaps with all astrocytes (left, arrow) and a small subset of neurons (middle, asterisk) in V1, with no overlap seen with microglia (right, arrowhead). Cell-specific markers in red: Aldh1L1-EGFP for astrocytes, NeuN for neurons, Iba1 for microglia. Scale bar, 50 µm. (E) The rarely occurring GFAP+ astrocytes (red) in healthy visual cortex also express hevin (green). Scale bar, 10 µm.DOI:https://dx.doi.org/10.7554/eLife.04047.003 Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25517933), licensed under a CC-BY license. Not internally tested by R&amp;D Systems." class="cust_viewed_9" > <img loading="lazy" src="https://images.novusbio.com/images/af2836_mouse-sparc-like-1-sparcl1-affinity-purified-polyclonal-ab-4120241239329.jpg" alt="Western Blot SPARC-like 1/SPARCL1 Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/sparc-like-1-sparcl1-antibody_af2836" title="Hevin expression by astrocytes is developmentally regulated in the cortex.(A) Representative Western blots showing the developmental timeline for hevin expression in mouse cortex and hippocampus (tubulin was used as a loading control). (B) Quantification of Western blot analysis of hevin expression shows high expression between P15–P25. Data is presented as fold change compared to P1 levels (n = 3 animals per age; p &lt; 0.05; one-way ANOVA with Dunnett's post hoc test). (C) Schematic diagram of a coronal slice through mouse brain shows the synaptic zone of primary visual cortex (V1) where EM, IHC and Golgi-cox staining analyses were performed. Layer II/III neurons of the visual cortex heavily project their dendrites to this region (D) IHC staining reveals that hevin expression (green) overlaps with all astrocytes (left, arrow) and a small subset of neurons (middle, asterisk) in V1, with no overlap seen with microglia (right, arrowhead). Cell-specific markers in red: Aldh1L1-EGFP for astrocytes, NeuN for neurons, Iba1 for microglia. Scale bar, 50 µm. (E) The rarely occurring GFAP+ astrocytes (red) in healthy visual cortex also express hevin (green). Scale bar, 10 µm.DOI:https://dx.doi.org/10.7554/eLife.04047.003 Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25517933), licensed under a CC-BY license. Not internally tested by R&amp;D Systems." class="cust_viewed_9" > <img loading="lazy" src="https://images.novusbio.com/images/af2836_mouse-sparc-like-1-sparcl1-affinity-purified-polyclonal-ab-41202412401258.jpg" alt="Immunocytochemistry/ Immunofluorescence SPARC-like 1/SPARCL1 Antibody" class="big_thumb" /> </a> </div></div><div id="ss_next_cav9" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-9" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/sparc-like-1-sparcl1-antibody_af2836#PublicationSection"> <div class="sprite_pubBook"></div> (17 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Mu</div><div class="tiny"><strong>Applications:</strong> WB</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="MAB4219"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/claudin-4-antibody-382321_mab4219" style="overflow: hidden; width: 280px;" title="Claudin-4 Antibody (382321) [Unconjugated]">Claudin-4 Antibody<br> (382321) [Unconjugate ...</a> <!--<div class="purchase_cart_wrapper"> <div class="purchase_cart btnAdd" data-type="anc_btnonly" data-catbase="MAB4219" data-return="synaptic-glycoprotein-sc2-antibody_nbp2-49230#datasheet" data-produrl="synaptic-glycoprotein-sc2-antibody_nbp2-49230" data-catnums="MAB4219" data-baseurl="https://www.novusbio.com" data-productid="411373"></div>--> <!-- <a href="/commerce/add-to-cart/411373"><div class="purchase_cart"></div></a> --> <!--</div>--> </div><div class="sub-header">MAB4219</div> <div class="images_customers"><div class="one_image_l"></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-10" data-cycle-prev="#ss_prev_cav10" data-cycle-next="#ss_next_cav10" data-cycle-slides="div.big_thumb_cust_container" data-index=10 ><div class="big_thumb_cust_container"> <a href="/products/claudin-4-antibody-382321_mab4219" title="PC-3 human prostate cancer cell line was stained with Mouse Anti-Human Claudin-4 Monoclonal Antibody (Catalog # MAB4219, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram) followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins." class="cust_viewed_10" > <img loading="lazy" src="https://images.novusbio.com/images2/Claudin4_MAB4219_Flow_Cytometry_21352.jpg" alt="Flow Cytometry Claudin-4 Antibody (382321) [Unconjugated]" class="big_thumb" /> </a> </div></div><div class="one_image"></div><div class="one_image_11"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/claudin-4-antibody-382321_mab4219#PublicationSection"> <div class="sprite_pubBook"></div> (2 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu</div><div class="tiny"><strong>Applications:</strong> CyTOF-ready, Flow</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NBP1-56853"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/rpe-antibody_nbp1-56853" style="overflow: hidden; width: 280px;" title="RPE Antibody">RPE Antibody</a> <!--<div class="purchase_cart_wrapper"> <div class="purchase_cart btnAdd" data-type="anc_btnonly" data-catbase="NBP1-56853" data-return="synaptic-glycoprotein-sc2-antibody_nbp2-49230#datasheet" data-produrl="synaptic-glycoprotein-sc2-antibody_nbp2-49230" data-catnums="NBP1-56853" data-baseurl="https://www.novusbio.com" data-productid="226695"></div>--> <!-- <a href="/commerce/add-to-cart/226695"><div class="purchase_cart"></div></a> --> <!--</div>--> </div><div class="sub-header">NBP1-56853</div> <div class="images_customers"><div id="ss_prev_cav11" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-11" data-cycle-prev="#ss_prev_cav11" data-cycle-next="#ss_next_cav11" data-cycle-slides="div.big_thumb_cust_container" data-index=11 ><div class="big_thumb_cust_container"> <a href="/products/rpe-antibody_nbp1-56853" title="Western Blot: RPE Antibody [NBP1-56853] - Human Muscle lysate, concentration 0.2-1 ug/ml." class="cust_viewed_11" > <img loading="lazy" src="https://images.novusbio.com/images/RPE-Antibody-Western-Blot-NBP1-56853-img0002.jpg" alt="Western Blot RPE Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/rpe-antibody_nbp1-56853" title="Immunohistochemistry-Paraffin: RPE Antibody [NBP1-56853] - Antibody 0 Formalin Fixed Paraffin Embedded Tissue: Human Adult Liver Observed Staining: Cytoplasm but only in connective tissue cells in the interlobular septum, very low tissue distribution Primary Antibody" class="cust_viewed_11" > <img loading="lazy" src="https://images.novusbio.com/images/RPE-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-56853-img0003.jpg" alt="Immunohistochemistry-Paraffin RPE Antibody" class="big_thumb" /> </a> </div></div><div id="ss_next_cav11" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-11" class="cycle-circle-pager"></div></div> <div class="ds_info"><div class="tiny"><strong>Species:</strong> Hu</div><div class="tiny"><strong>Applications:</strong> IHC, IHC-P, WB</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="H00006941-M01"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/tcf19-antibody-6d8_h00006941-m01" style="overflow: hidden; width: 280px;" title="TCF19 Antibody (6D8)">TCF19 Antibody<br> (6D8)</a> <!--<div class="purchase_cart_wrapper"> <div class="purchase_cart btnAdd" data-type="anc_btnonly" data-catbase="H00006941-M01" data-return="synaptic-glycoprotein-sc2-antibody_nbp2-49230#datasheet" data-produrl="synaptic-glycoprotein-sc2-antibody_nbp2-49230" data-catnums="H00006941-M01" data-baseurl="https://www.novusbio.com" data-productid="5087"></div>--> <!-- <a href="/commerce/add-to-cart/5087"><div class="purchase_cart"></div></a> --> <!--</div>--> </div><div class="sub-header">H00006941-M01</div> <div class="images_customers"><div id="ss_prev_cav12" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-12" data-cycle-prev="#ss_prev_cav12" data-cycle-next="#ss_next_cav12" data-cycle-slides="div.big_thumb_cust_container" data-index=12 ><div class="big_thumb_cust_container"> <a href="/products/tcf19-antibody-6d8_h00006941-m01" title="Immunocytochemistry/Immunofluorescence: TCF19 Antibody (6D8) [H00006941-M01] - Analysis of monoclonal antibody to TCF19 on HeLa cell. Antibody concentration 10 ug/ml." class="cust_viewed_12" > <img loading="lazy" src="https://images.novusbio.com/images/TCF19-Antibody-6D8-Immunocytochemistry-Immunofluorescence-H00006941-M01-img0008.jpg" alt="Immunocytochemistry/ Immunofluorescence TCF19 Antibody (6D8)" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/tcf19-antibody-6d8_h00006941-m01" title="Immunohistochemistry-Paraffin: TCF19 Antibody (6D8) [H00006941-M01] - Analysis of monoclonal antibody to TCF19 on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml." class="cust_viewed_12" > <img loading="lazy" src="https://images.novusbio.com/images/TCF19-Antibody-6D8-Immunohistochemistry-Paraffin-H00006941-M01-img0010.jpg" alt="Immunohistochemistry-Paraffin TCF19 Antibody (6D8)" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/tcf19-antibody-6d8_h00006941-m01" title="Western Blot: TCF19 Antibody (6D8) [H00006941-M01] - Analysis of TCF19 over-expressed 293 cell line, cotransfected with TCF19 Validated Chimera RNAi ( Cat # H00006941-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TCF19 monoclonal antibody (M01), clone 6D8 (Cat # H00006941-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control." class="cust_viewed_12" > <img loading="lazy" src="https://images.novusbio.com/images/TCF19-Antibody-6D8-Western-Blot-H00006941-M01-img0011.jpg" alt="Western Blot TCF19 Antibody (6D8)" class="big_thumb" /> </a> </div></div><div id="ss_next_cav12" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-12" class="cycle-circle-pager"></div></div> <div class="ds_info"><div class="tiny"><strong>Species:</strong> Hu</div><div class="tiny"><strong>Applications:</strong> ELISA, ICC/IF, IHC, IHC-P, KD, WB</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NBP2-67486"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/myosin-light-chain-kinase-antibody-su40-06_nbp2-67486" style="overflow: hidden; width: 280px;" title="Myosin light chain kinase Antibody (SU40-06)">Myosin light chain<br> kinase Antibody (SU40 ...</a> <!--<div class="purchase_cart_wrapper"> <div class="purchase_cart btnAdd" data-type="anc_btnonly" data-catbase="NBP2-67486" data-return="synaptic-glycoprotein-sc2-antibody_nbp2-49230#datasheet" data-produrl="synaptic-glycoprotein-sc2-antibody_nbp2-49230" data-catnums="NBP2-67486" data-baseurl="https://www.novusbio.com" data-productid="631259"></div>--> <!-- <a href="/commerce/add-to-cart/631259"><div class="purchase_cart"></div></a> --> <!--</div>--> </div><div class="sub-header">NBP2-67486</div> <div class="images_customers"><div id="ss_prev_cav13" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-13" data-cycle-prev="#ss_prev_cav13" data-cycle-next="#ss_next_cav13" data-cycle-slides="div.big_thumb_cust_container" data-index=13 ><div class="big_thumb_cust_container"> <a href="/products/myosin-light-chain-kinase-antibody-su40-06_nbp2-67486" title="Flow Cytometry: Myosin light chain kinase Antibody (SU40-06) [NBP2-67486] - Analysis of SH-SY-5Y cells with MYLK antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody." class="cust_viewed_13" > <img loading="lazy" src="https://images.novusbio.com/images/Myosin-light-chain-kinase-Antibody-SU40-06-Flow-Cytometry-NBP2-67486-img0001.jpg" alt="Flow Cytometry Myosin light chain kinase Antibody (SU40-06)" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/myosin-light-chain-kinase-antibody-su40-06_nbp2-67486" title="Immunocytochemistry/Immunofluorescence: Myosin light chain kinase Antibody (SU40-06) [NBP2-67486] - Staining MYLK in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS." class="cust_viewed_13" > <img loading="lazy" src="https://images.novusbio.com/images/Myosin-light-chain-kinase-Antibody-SU40-06-Immunocytochemistry-Immunofluorescence-NBP2-67486-img0002.jpg" alt="Immunocytochemistry/ Immunofluorescence Myosin light chain kinase Antibody (SU40-06)" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/myosin-light-chain-kinase-antibody-su40-06_nbp2-67486" title="Immunocytochemistry/Immunofluorescence: Myosin light chain kinase Antibody (SU40-06) [NBP2-67486] - Staining MYLK in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS." class="cust_viewed_13" > <img loading="lazy" src="https://images.novusbio.com/images/Myosin-light-chain-kinase-Antibody-SU40-06-Immunocytochemistry-Immunofluorescence-NBP2-67486-img0003.jpg" alt="Immunocytochemistry/ Immunofluorescence Myosin light chain kinase Antibody (SU40-06)" class="big_thumb" /> </a> </div></div><div id="ss_next_cav13" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-13" class="cycle-circle-pager"></div></div> <div class="ds_info"><div class="tiny"><strong>Species:</strong> Hu, Mu, Rt</div><div class="tiny"><strong>Applications:</strong> Flow, ICC/IF, IHC, IHC-P, WB</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="AF748"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/e-cadherin-antibody_af748" style="overflow: hidden; width: 280px;" title="E-Cadherin Antibody [Unconjugated]">E-Cadherin<br> Antibody [Unconjugate</a> <!--<div class="purchase_cart_wrapper"> <div class="purchase_cart btnAdd" data-type="anc_btnonly" data-catbase="AF748" data-return="synaptic-glycoprotein-sc2-antibody_nbp2-49230#datasheet" data-produrl="synaptic-glycoprotein-sc2-antibody_nbp2-49230" data-catnums="AF748" data-baseurl="https://www.novusbio.com" data-productid="412872"></div>--> <!-- <a href="/commerce/add-to-cart/412872"><div class="purchase_cart"></div></a> --> <!--</div>--> </div><div class="sub-header">AF748</div> <div class="images_customers"><div id="ss_prev_cav14" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-14" data-cycle-prev="#ss_prev_cav14" data-cycle-next="#ss_next_cav14" data-cycle-slides="div.big_thumb_cust_container" data-index=14 ><div class="big_thumb_cust_container"> <a href="/products/e-cadherin-antibody_af748" title="E-Cadherin was detected in immersion fixed D3 mouse embryonic stem cell line using Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights&amp;#8482; 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips." class="cust_viewed_14" > <img loading="lazy" src="https://images.novusbio.com/images2/ECadherin_AF748_Immunocytochemistry_15885.jpg" alt="Immunocytochemistry E-Cadherin Antibody [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/e-cadherin-antibody_af748" title="E‑Cadherin was detected in perfusion fixed frozen sections of mouse skin using Goat Anti-Human/Mouse E‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748) at 1.7&amp;nbsp;µg/mL overnight at 4&amp;nbsp;°C. Tissue was stained using the NorthernLights&amp;#8482; 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog&amp;nbsp;# NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes in keratinocytes. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections. " class="cust_viewed_14" > <img loading="lazy" src="https://images.novusbio.com/images2/ECadherin_AF748_Immunohistochemistry_15886.jpg" alt="Immunohistochemistry E-Cadherin Antibody [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/e-cadherin-antibody_af748" title="E-Cadherin was detected in immersion fixed mouse intestinal organoids using Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights&amp;#8482; 557-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL001) and counterstained with DAPI (blue). Magnification shown at 100X (upper panel) and 40X (lower panel). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="cust_viewed_14" > <img loading="lazy" src="https://images.novusbio.com/images2/ECadherin_AF748_Immunocytochemistry_16572.jpg" alt="Immunocytochemistry E-Cadherin Antibody [Unconjugated]" class="big_thumb" /> </a> </div></div><div id="ss_next_cav14" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-14" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/e-cadherin-antibody_af748#PublicationSection"> <div class="sprite_pubBook"></div> (142 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu, Mu</div><div class="tiny"><strong>Applications:</strong> CyTOF-ready, Dual ISH-IHC, Flow, ICC, IHC, Simple Western, WB</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NBP2-62682"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/mylk3-antibody_nbp2-62682" style="overflow: hidden; width: 280px;" title="MYLK3 Antibody">MYLK3 Antibody</a> <!--<div class="purchase_cart_wrapper"> <div class="purchase_cart btnAdd" data-type="anc_btnonly" data-catbase="NBP2-62682" data-return="synaptic-glycoprotein-sc2-antibody_nbp2-49230#datasheet" data-produrl="synaptic-glycoprotein-sc2-antibody_nbp2-49230" data-catnums="NBP2-62682" data-baseurl="https://www.novusbio.com" data-productid="623531"></div>--> <!-- <a href="/commerce/add-to-cart/623531"><div class="purchase_cart"></div></a> --> <!--</div>--> </div><div class="sub-header">NBP2-62682</div> <div class="images_customers"><div id="ss_prev_cav15" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-15" data-cycle-prev="#ss_prev_cav15" data-cycle-next="#ss_next_cav15" data-cycle-slides="div.big_thumb_cust_container" data-index=15 ><div class="big_thumb_cust_container"> <a href="/products/mylk3-antibody_nbp2-62682" title="Immunohistochemistry-Paraffin: MYLK3 Antibody [NBP2-62682] - Analysis in human heart muscle and pancreas tissues using Anti-MYLK3 antibody. Corresponding MYLK3 RNA-seq data are presented for the same tissues." class="cust_viewed_15" > <img loading="lazy" src="https://images.novusbio.com/images/MYLK3-Antibody-Immunohistochemistry-Paraffin-NBP2-62682-img0001.jpg" alt="Immunohistochemistry-Paraffin MYLK3 Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/mylk3-antibody_nbp2-62682" title="Immunohistochemistry-Paraffin: MYLK3 Antibody [NBP2-62682] - Staining of human pancreas shows low expression as expected." class="cust_viewed_15" > <img loading="lazy" src="https://images.novusbio.com/images/MYLK3-Antibody-Immunohistochemistry-Paraffin-NBP2-62682-img0003.jpg" alt="Immunohistochemistry-Paraffin MYLK3 Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/mylk3-antibody_nbp2-62682" title="Immunohistochemistry-Paraffin: MYLK3 Antibody [NBP2-62682] - Staining of human heart muscle shows high expression." class="cust_viewed_15" > <img loading="lazy" src="https://images.novusbio.com/images/MYLK3-Antibody-Immunohistochemistry-Paraffin-NBP2-62682-img0002.jpg" alt="Immunohistochemistry-Paraffin MYLK3 Antibody" class="big_thumb" /> </a> </div></div><div id="ss_next_cav15" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-15" class="cycle-circle-pager"></div></div> <div class="ds_info"><div class="tiny"><strong>Species:</strong> Hu</div><div class="tiny"><strong>Applications:</strong> IHC, IHC-P</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="MAB25031"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/cftr-antibody-24-1_mab25031" style="overflow: hidden; width: 280px;" title="CFTR Antibody (24-1) [Unconjugated] - C-terminus">CFTR Antibody<br> (24-1) [Unconjugated] ...</a> <!--<div class="purchase_cart_wrapper"> <div class="purchase_cart btnAdd" data-type="anc_btnonly" data-catbase="MAB25031" data-return="synaptic-glycoprotein-sc2-antibody_nbp2-49230#datasheet" data-produrl="synaptic-glycoprotein-sc2-antibody_nbp2-49230" data-catnums="MAB25031" data-baseurl="https://www.novusbio.com" data-productid="417497"></div>--> <!-- <a href="/commerce/add-to-cart/417497"><div class="purchase_cart"></div></a> --> <!--</div>--> </div><div class="sub-header">MAB25031</div> <div class="images_customers"><div id="ss_prev_cav16" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-16" data-cycle-prev="#ss_prev_cav16" data-cycle-next="#ss_next_cav16" data-cycle-slides="div.big_thumb_cust_container" data-index=16 ><div class="big_thumb_cust_container"> <a href="/products/cftr-antibody-24-1_mab25031" title="CFTR was detected in immersion fixed paraffin-embedded sections of human placenta using 8 µg/mL Mouse Anti-Human CFTR C-Terminus Monoclonal Antibody (Catalog # MAB25031) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-AEC Cell &amp; Tissue Staining Kit (red; Catalog # CTS003) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="cust_viewed_16" > <img loading="lazy" src="https://images.novusbio.com/images2/CFTR_MAB25031_Immunohistochemistry_6973.jpg" alt="Immunohistochemistry CFTR Antibody (24-1) [Unconjugated] - C-terminus" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/cftr-antibody-24-1_mab25031" title="CFTR was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human CFTR C-Terminus Monoclonal Antibody (Catalog # MAB25031) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell &amp; Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="cust_viewed_16" > <img loading="lazy" src="https://images.novusbio.com/images2/CFTR_MAB25031_Immunohistochemistry_9671.jpg" alt="Immunohistochemistry CFTR Antibody (24-1) [Unconjugated] - C-terminus" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/cftr-antibody-24-1_mab25031" title="COMMD1 and CFTR interact in mammalian cells.(A) Sequences of ICL3 in other species from fish to primates. Asterisks indicate the position of two class II mutations: S945L and D979A. Identity of amino acids between the different proteins are boxed in black, conserved residues are boxed in dark gray and semi-conserved substitutions in light gray. (B) Representative gels for the same co-immunoprecipitation experiments in HT-29 cells expressing endogenous CFTR and COMMD1. Lysates from HT-29 cells were immunoprecipitated (IP) with either 0.8 µg of anti-COMMD1 mAb (Abnova), 0.8 µg of anti-CFTR mAb (MAB25031, R&amp;D Systems) or with 0.8 µg anti-mouse IgG as a control. Each immunoprecipitation sample was then split in half and loaded onto an 8% SDS-PAGE for CFTR detection and 11% SDS-PAGE for COMMD1 detection. Both gels were transferred to PVDF membrane and subjected to immunoblotting (IB). The 8% SDS-PAGE membrane was probed with anti-CFTR mAb (MM13-4) and the 11% SDS-PAGE membrane with a rabbit anti-COMMD1 pAb (Proteintech Group). Both membranes were probed with anti-alpha -tubulin as control (11% gel is shown). Filled and empty arrowheads indicate the fully- (170 kDa) and core-glycosylated (140 kDa) CFTR, respectively. * indicates mouse IgG light chain from the antibody used for immunoprecipitation. (C) COMMD1 constructions in pcDNA3.1/Topo plasmid. Two COMMD1 constructs were generated by adding a Myc-tag at the N-terminus of COMMD1: Myc-COMMD1 and a construct with a deletion of the COMM domain named Myc-COMMD1 delta COMM. (D) Representative gels for the same co-immunoprecipitation experiment between COMMD1 and wt- in heterologous system. HeLa cells stably expressing wt- (spTCF-wt) or empty CFTR vector (spTracer) as control were transfected with Myc-COMMD1. spTCF-wt were transfected with Myc-COMMD1 delta COMM. Lysates from all these experiments were subjected to SDS-PAGE, as in (B) after CFTR IP. The 8% SDS-PAGE membrane was probed with anti-CFTR mAb and the 11% SDS-PAGE membrane with anti-c-Myc mAb. Both membranes were probed with anti-alpha -tubulin as control (11% gel is shown). Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0018334), licensed under a CC-BY license. Not internally tested by R&amp;D Systems." class="cust_viewed_16" > <img loading="lazy" src="https://images.novusbio.com/images/mab25031_human-cftr-c-terminus-mab-clone-24-1-41202412513815.jpg" alt="Immunoprecipitation CFTR Antibody (24-1) [Unconjugated] - C-terminus" class="big_thumb" /> </a> </div></div><div id="ss_next_cav16" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-16" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/cftr-antibody-24-1_mab25031#ReviewsSection"> <div class="sprite_4Stars"></div> (10 Reviews</a>) </div> <div class="tiny"> <a href="/products/cftr-antibody-24-1_mab25031#PublicationSection"> <div class="sprite_pubBook"></div> (76 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu</div><div class="tiny"><strong>Applications:</strong> IHC, IP, WB</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NBP1-81555"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/mlc1-antibody_nbp1-81555" style="overflow: hidden; width: 280px;" title="MLC1 Antibody">MLC1 Antibody</a> <!--<div class="purchase_cart_wrapper"> <div class="purchase_cart btnAdd" data-type="anc_btnonly" data-catbase="NBP1-81555" data-return="synaptic-glycoprotein-sc2-antibody_nbp2-49230#datasheet" data-produrl="synaptic-glycoprotein-sc2-antibody_nbp2-49230" data-catnums="NBP1-81555" data-baseurl="https://www.novusbio.com" data-productid="280393"></div>--> <!-- <a href="/commerce/add-to-cart/280393"><div class="purchase_cart"></div></a> --> <!--</div>--> </div><div class="sub-header">NBP1-81555</div> <div class="images_customers"><div id="ss_prev_cav17" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-17" data-cycle-prev="#ss_prev_cav17" data-cycle-next="#ss_next_cav17" data-cycle-slides="div.big_thumb_cust_container" data-index=17 ><div class="big_thumb_cust_container"> <a href="/products/mlc1-antibody_nbp1-81555" title="Western Blot: MLC1 Antibody [NBP1-81555] - Analysis in control (vector only transfected HEK293T lysate) and MLC1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells)." class="cust_viewed_17" > <img loading="lazy" src="https://images.novusbio.com/images/MLC1-Antibody-Western-Blot-NBP1-81555-img0008.jpg" alt="Western Blot MLC1 Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/mlc1-antibody_nbp1-81555" title="Immunohistochemistry-Paraffin: MLC1 Antibody [NBP1-81555] - Staining of human cerebellum shows moderate cytoplasmic positivity in neuropil of the molecular layer." class="cust_viewed_17" > <img loading="lazy" src="https://images.novusbio.com/images/MLC1-Antibody-Immunohistochemistry-Paraffin-NBP1-81555-img0009.jpg" alt="Immunohistochemistry-Paraffin MLC1 Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/mlc1-antibody_nbp1-81555" title="Immunohistochemistry-Paraffin: MLC1 Antibody [NBP1-81555] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neuropil." class="cust_viewed_17" > <img loading="lazy" src="https://images.novusbio.com/images/MLC1-Antibody-Immunohistochemistry-Paraffin-NBP1-81555-img0010.jpg" alt="Immunohistochemistry-Paraffin MLC1 Antibody" class="big_thumb" /> </a> </div></div><div id="ss_next_cav17" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-17" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/mlc1-antibody_nbp1-81555#PublicationSection"> <div class="sprite_pubBook"></div> (2 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu</div><div class="tiny"><strong>Applications:</strong> IHC, IHC-P, WB</div> </div> </div> </div></div> </div><div id="cav_next"><div class="sprite_rightArrow"></div></div> <div class="clear"></div></div> <div class="ds_tab_content equalbottom" id="content_reviews-publications"> <a id="reviews-publications"></a> <div> <a id="PublicationSection"></a> <h2 class="greyHead2">Publications for Synaptic glycoprotein SC2 Antibody (NBP2-49230) (0)</h2> </div> <div> <div class="ds_info">There are no publications for Synaptic glycoprotein SC2 Antibody (NBP2-49230).</div> <div class="ds_info">By submitting your publication information earn gift cards and discounts for future purchases.</div> </div> <div> <div class="align-right"> <button class="greybutton btn_width" id="novuspublication" name="novuspublication" onclick="window.location='/publications'"; >Submit a Publication</button><br /> </div> </div> <a id="ReviewsSection"></a> <h2 class="greyHead2">Reviews for <span>Synaptic glycoprotein SC2 Antibody (NBP2-49230) (0) </span></h2> <div class="ds_info"> There are no reviews for Synaptic glycoprotein SC2 Antibody (NBP2-49230). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount. <br/> <ul> <li>Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen</li> <li>Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen</li> </ul> <div class="align-right"> <a href="/reviews/NBP2-49230"><button class="greybutton btn_width" name="novusreview">Submit a Review</button></a><br /> </div> </div> <div class="clear"></div></div> <div class="ds_tab_content equalbottom" id="content_protocols-faqs"> <a id="protocols-faqs"></a><h2 class="greyHead2">Product General Protocols</h2> <a id="protocols"></a> <div class="ds_info">Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars. <ul><li><a href="/support/support-by-application/immunocytochemistry/protocol.html">Immunocytochemistry/ Immunofluorescence</a></li><li><a href="/support/support-by-application/immunohistochemistry-paraffin/protocol.html">Immunohistochemistry-Paraffin</a></li><li><a href="/support/support-by-application/western-blot/protocol.html">Western Blot</a></li><li><a href="/support/support-by-application.html">View all Protocols, Troubleshooting, Illustrated assays and Webinars.</a></li> </ul></div><h2 class="greyHead2 ds_info">Video Protocols</h2> <a id="videos"></a> <div class="ds_info"><div class="movie-cell"> <span class="movie-title">WB Video Protocol</span><br/> <meta content ="https://www.youtube.com/watch?v=uFu8aie4QFI" /> <a href="https://www.youtube.com/embed/uFu8aie4QFI?rel=0&wmode=transparent" class="youtube"> <div class="reduce sprite_WBvideo"></div> </a> </div><div class="movie-cell"> <span class="movie-title">ICC/IF Video Protocol</span><br/> <meta content ="https://www.youtube.com/watch?v=5AcrhhHmQZs" /> <a href="https://www.youtube.com/embed/5AcrhhHmQZs?rel=0&wmode=transparent" class="youtube"> <div class="reduce sprite_ICC-IFvideo"></div> </a> </div></div> <div class="clear"></div><h2 class="greyHead2">FAQs for Synaptic glycoprotein SC2 Antibody (NBP2-49230) (0)</h2> <a id="faqs"></a> <div class="ds_info">There are no specific FAQs related to this product. Read our general <a href="/support/faqs.html">customer & technical service FAQs.</a> </div> <div class="clear"></div></div> <div class="ds_tab_content equalbottom" id="content_supportresearch"> <a id="supportresearch"></a><table cellpadding="0" cellspacing="0"><tr><td valign="top" class="width50"> <h2 class="greyHead2">Secondary Antibodies</h2> <a id="secondaryantibodies"></a> <div class="ds_info"> <ul class="information-list"><li><a href="/product-type/secondary-antibodies?species=Rabbit&facet_conjugate=HRP">Anti-Rabbit HRP Labeled Antibodies</a></li><li><a href="/product-type/secondary-antibodies?species=Rabbit&facet_conjugate=FITC">Anti-Rabbit FITC Labeled Antibodies</a></li><li><a href="/product-type/secondary-antibodies?species=Rabbit&facet_conjugate=Biotin">Anti-Rabbit Biotin Labeled Antibodies</a></li><li> </li> <li><a href="/product-type/secondary-antibodies#fq=species%3A%22Rabbit%22">View all of our anti-Rabbit Secondary Antibodies</a></li> </ul> </div></td><td width="5"> </td><td valign="top"> <h2 class="greyHead2" >Isotype Controls</h2> <a id="isotypecontrols"></a> <div class="ds_info"> <ul class="ds_noDotList"><li><a href="/product-type/isotype-controls#fq=species%3A%22Rabbit%22">Rabbit Isotype Controls</a></li><li> </li> <li><a href="/product-type/isotype-controls">View all of our Isotype Control Products</a></li> </div></td></tr></table><h2 class="greyHead2">Additional Synaptic glycoprotein SC2 Products</h2> <a id="addtlalphaprods"></a> <div class="ds_info"> <div> <span style="display:none;">Array NBP2-49230</span> <ul class="ds_noDotList"><li> <a href="/primary-antibodies/synaptic-glycoprotein-sc2">Synaptic glycoprotein SC2 Antibodies</a></li><li> <a href="/lysates/synaptic-glycoprotein-sc2">Synaptic glycoprotein SC2 Lysate</a></li><li> <a href="/proteins/synaptic-glycoprotein-sc2">Synaptic glycoprotein SC2 Proteins</a></li></ul> </div> </div><table cellpadding="0" cellspacing="0" class="width100"><tr></tr></table><table cellpadding="0" cellspacing="0" class="width100"><tr></tr></table><h2 class="greyHead2">Blogs on Synaptic glycoprotein SC2</h2> <a id="blogsect"></a> <div>There are no specific blogs for Synaptic glycoprotein SC2, but you can <a href="/antibody-news/">read our latest blog posts.</div> <div class="clear"></div></div> </div></div><div class="grid_6"><div class="ds_blue_border marTop26 equalbottom"><div class="datasheet-ads-container" ><a href="/products/mfluor-violet-fluorescent-conjugated-antibodies" target="_parent"><img loading="lazy" src="https://images.novusbio.com/design/mfluor-violet-conjugated-antibodies-banner-final-v5-349x119.jpg" alt="mFluor Violet Conjugated Antibodies" title="mFluor Violet Conjugated Antibodies" class="datasheet-adzone" loading="lazy"></a></div><a id="jump_recommended"></a> <h2 class="greyHead2">Customers Who Bought This Also Bought</h2> <div class="text_center"><div class="images2"><div id="ss_prev_sidebar" class="cycle-prev ds_rp_prev"><div class="pager_left_on"></div></div><div id="ss_next_sidebar" class="cycle-next ds_rp_next"><div class="pager_right_on"></div></div><div class="cycle-slideshow" data-cycle-fx="scrollHorz" data-cycle-timeout="0" data-cycle-prev="#ss_prev_sidebar" data-cycle-next="#ss_next_sidebar" data-cycle-slides="div.big_thumb_container" id="ds_customer_who_bought" ><div class="big_thumb_container" data-catnums="HAF008"> <div class="img_container"> <a href="/products/igg-antibody_haf008" title="Western blot shows detection of Rabbit Anti-Human/Mouse/Rat HSP60 Polyclonal Antibody (AF1800) using a 1:1000 dilution of HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="cust_bought" > <img loading="lazy" src="https://images.novusbio.com/images2/IgG_HAF008_Western_Blot_19080.jpg" title="Western blot shows detection of Rabbit Anti-Human/Mouse/Rat HSP60 Polyclonal Antibody (AF1800) using a 1:1000 dilution of HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows detection of Rabbit Anti-Human/Mouse/Rat HSP60 Polyclonal Antibody (AF1800) using a 1:1000 dilution of HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" /> </a> </div> <div class="caption_container"><a href="/products/igg-antibody_haf008">Goat anti-Rabbit IgG Secondary Antibody [HRP]</a> <br />HAF008</div> <div style="text-align: right"><button class="greybutton" id="novusviewdatasheet" name="novusviewdatasheet" onclick="window.location='/products/igg-antibody_haf008';" >View Datasheet</button> </div> </div><div class="big_thumb_container" data-catnums="NB7160"> <div class="img_container"> <a href="/products/igg-h-l-antibody_nb7160" title="Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242)." class="cust_bought" > <img loading="lazy" src="https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg" title="Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242)." alt="Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242)." class="big_thumb" /> </a> </div> <div class="caption_container"><a href="/products/igg-h-l-antibody_nb7160">Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP]</a> <br />NB7160</div> <div style="text-align: right"><button class="greybutton" id="novusviewdatasheet" name="novusviewdatasheet" onclick="window.location='/products/igg-h-l-antibody_nb7160';" >View Datasheet</button> </div> </div><div class="big_thumb_container" data-catnums="NBP2-24891"> <div class="img_container"> <a href="/products/igg-isotype-control_nbp2-24891" title="Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary." class="cust_bought" > <img loading="lazy" src="https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg" title="Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary." alt="Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. 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