ERK2 Antibody [Unconjugated] (AF1230): Novus Biologicals

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5.1724696046-473375462.1712005244" style="margin: 0 10px;" target="_blank"><img alt="Novus Biologicals products are now on bio-techne.com" src="https://images.novusbio.com/design/ready-to-buy-novus-products-login.png" style="height:120px; width:940px" /></a></div> </div> </div> </div> <h1>ERK2 Antibody [Unconjugated]</h1> </div> <div class="clear"></div> <div> <div class="region region-content"> <div id="block-et-messages-et-messages-block" class="block block-et-messages"> <div class="content"> <span></span> </div> </div> <div id="block-system-main" class="block block-system"> <div class="content"> <div class="grid_16"><div class="ds_info float_left"><a href="#PublicationSection"><div class="sprite_pubBook_large"></div></a> <a href="#PublicationSection" class="bottom2"><span class="ctr">(3 Publications)</span></a></div><div class="ds_info float_left"> <a href="/common-name/erk2"><div class="sprite_allRelated"></div></a> <a href="/common-name/erk2" class="bottom4"><span class="ctr">See All Related</span></a> </div><div class="ds_info float_left"><a href="/reviews/AF1230"><div class="dsSprite_submitreview"></div></a> <a href="/reviews/AF1230" class="bottom4"><span class="ctr">Submit a Review</span></a></div></div></div><div class="modalWrapper" style="display:none;"> <h2>Species Glossary</h2> <hr class="customhr"/> <img src="//images.novusbio.com/design/bunny.gif" alt="Species Glossary Rabbit" style="float: right;"> <table class="modalTable"> <tr> <td>At</td> <td>=</td> <td>A. thaliana</td> <td>SyHa</td> <td>=</td> <td>Golden Syrian Hamster</td> </tr> <tr> <td>All</td> <td>=</td> <td>All Species</td> <td>Ha</td> <td>=</td> <td>Hamster</td> </tr> <tr> <td>An</td> <td>=</td> <td>Animal</td> <td>Hu</td> <td>=</td> <td>Human</td> </tr> <tr> <td>ArHa</td> <td>=</td> <td>Armenian Hamster</td> <td>I</td> <td>=</td> <td>Insect</td> </tr> <tr> <td>Av</td> <td>=</td> <td>Avian</td> <td>Ll</td> <td>=</td> <td>Llama</td> </tr> <tr> <td>Bb</td> <td>=</td> <td>Baboon</td> <td>Ma</td> <td>=</td> <td>Mammal</td> </tr> <tr> <td>Ba</td> <td>=</td> <td>Bacteria</td> <td>Mk</td> <td>=</td> <td>Monkey</td> </tr> <tr> <td>Bv</td> <td>=</td> <td>Bovine</td> <td>Mu</td> <td>=</td> <td>Mouse</td> </tr> <tr> <td>Ca</td> <td>=</td> <td>Canine</td> <td>Multi</td> <td>=</td> <td>Multi-species</td> </tr> <tr> <td>Ce</td> <td>=</td> <td>C. Elegans</td> <td>NA</td> <td>=</td> <td>Non-species specific</td> </tr> <tr> <td>Ch</td> <td>=</td> <td>Chicken</td> <td>Or</td> <td>=</td> <td>Orangutan</td> </tr> <tr> <td>ChHa</td> <td>=</td> <td>Chinese Hamster</td> <td>Pl</td> <td>=</td> <td>Plant</td> </tr> <tr> <td>Cm</td> <td>=</td> <td>Cynomolgus Monkey</td> <td>Pm</td> <td>=</td> <td>Primate</td> </tr> <tr> <td>Do</td> <td>=</td> <td>Donkey</td> <td>Po</td> <td>=</td> <td>Porcine</td> </tr> <tr> <td>Dr</td> <td>=</td> <td>Drosophilia</td> <td>Rb</td> <td>=</td> <td>Rabbit</td> </tr> <tr> <td>Ec</td> <td>=</td> <td>E. Coli</td> <td>Rt</td> <td>=</td> <td>Rat</td> </tr> <tr> <td>Eq</td> <td>=</td> <td>Equine</td> <td>RM</td> <td>=</td> <td>Rhesus Macaque</td> </tr> <tr> <td>Fe</td> <td>=</td> <td>Feline</td> <td>Sh</td> <td>=</td> <td>Sheep</td> </tr> <tr> <td>Ft</td> <td>=</td> <td>Ferret</td> <td>Vb</td> <td>=</td> <td>Vertebrate</td> </tr> <tr> <td>Fi</td> <td>=</td> <td>Fish</td> <td>Vi</td> <td>=</td> <td>Virus</td> </tr> <tr> <td>Fu</td> <td>=</td> <td>Fungi</td> <td>Xp</td> <td>=</td> <td>Xenopus</td> </tr> <tr> <td>Ge</td> <td>=</td> <td>Gerbil</td> <td>Ye</td> <td>=</td> <td>Yeast</td> </tr> <tr> <td>GP</td> <td>=</td> <td>Guinea Pig</td> <td>Ze</td> <td>=</td> <td>Zebrafish</td> </tr> <tr> <td>Gt</td> <td>=</td> <td>Goat</td> </tr> </table> <div style="text-align: center;margin-left:25px;margin-right:25px;"> <p>Note: Mouseover a species abbreviation on the product page to display the fullname.</p> </div> </div> </div><div class="clear"></div><div class="grid_10 pos_relative"><div class="ds_blue_border equaltop"><div class="imagesleft equaltop"><h2 class="blueHead">Images</h2> <div class="midBlue"> </div><div class="images"><div id="ss_prev_top" class="cycle-pager"><div class="pager_left_on"></div></div><div class="cycle-slideshow slideshows" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-top" data-cycle-prev="#ss_prev_top" data-cycle-next="#ss_next_top" data-cycle-caption="#image-caption" data-cycle-caption-template="Image {{slideNum}} of {{slideCount}}" data-cycle-slides=".big_thumb_container" data-cycle-carousel-visible="3" id="images_cycle" > <div class="sub_image_wrapper"><div id="cycle-circle-pager-top" class="cycle-circle-pager"></div><div id="image-caption"></div> <a class="enlargeTop">(<div class="dsSprite_magGlass"></div> Enlarge)</a></div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize2/ERK2_AF1230_Western_Blot_5097.jpg" class="top_images" rel="gallery" title="Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, U937 human histiocytic lymphoma cell line, PC-12 rat adrenal pheochromocytoma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse/Rat ERK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1230) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for ERK2 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." > <img src="//images.novusbio.com/images2/ERK2_AF1230_Western_Blot_5097.jpg" class="big_thumb" alt="Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, U937 human histiocytic lymphoma cell line, PC-12 rat adrenal pheochromocytoma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse/Rat ERK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1230) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for ERK2 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." title="Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, U937 human histiocytic lymphoma cell line, PC-12 rat adrenal pheochromocytoma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse/Rat ERK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1230) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for ERK2 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." /> </a> </div> <div class="alt-caption padding_left5" >Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, U937 human histiocytic lymphoma cell line, PC-12 rat adrenal pheochromocytoma cell line, and NIH-3T3<a class="openGallery" href="#" data-picnum="0"> ...read more</a></div> </div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize2/ERK2_AF1230_Immunohistochemistry_20696.jpg" class="top_images" rel="gallery" title=" ERK2 was detected in immersion fixed paraffin-embedded sections of human breast using Rabbit Anti-Human/Mouse/Rat ERK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1230) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents. " > <img src="//images.novusbio.com/images2/ERK2_AF1230_Immunohistochemistry_20696.jpg" loading="lazy" style="display:none;" class="big_thumb" alt=" ERK2 was detected in immersion fixed paraffin-embedded sections of human breast using Rabbit Anti-Human/Mouse/Rat ERK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1230) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents. " title=" ERK2 was detected in immersion fixed paraffin-embedded sections of human breast using Rabbit Anti-Human/Mouse/Rat ERK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1230) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents. " /> </a> </div> <div class="alt-caption padding_left5" > ERK2 was detected in immersion fixed paraffin-embedded sections of human breast using Rabbit Anti-Human/Mouse/Rat ERK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1230) at 3 µg/mL for 1 hour at<a class="openGallery" href="#" data-picnum="1"> ...read more</a></div> </div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize2/ERK2_AF1230_Knockout_Validated_22864.jpg" class="top_images" rel="gallery" title="<b>Genetic Strategies Validation. </b>Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and ERK2 knockout HeLa cell line (KO). PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse/Rat ERK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1230) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for ERK2 at approximately 42 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." > <img src="//images.novusbio.com/images2/ERK2_AF1230_Knockout_Validated_22864.jpg" loading="lazy" style="display:none;" class="big_thumb" alt="Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and ERK2 knockout HeLa cell line (KO). PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse/Rat ERK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1230) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for ERK2 at approximately 42 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." title="Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and ERK2 knockout HeLa cell line (KO). PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse/Rat ERK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1230) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for ERK2 at approximately 42 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." /> </a> </div> <div class="alt-caption padding_left5" ><b>Genetic Strategies</b>: Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and ERK2 knockout HeLa cell line (KO). PVDF membrane was probed with 0.1 µg/mL of Rabbit<a class="openGallery" href="#" data-picnum="2"> ...read more</a></div> </div></div><div id="ss_next_top" class="cycle-pager"><div class="pager_right_on"></div></div></div></div><div class="productsright pos_relative equaltop"><h2 class="blueHead">Product Details</h2> <div class="midBlue">Summary</div><table class="ds_list"><tr class="lined"> <td><strong>Reactivity</strong></td> <td><span title='Human'>Hu</span>, <span title='Mouse'>Mu</span>, <span title='Rat'>Rt</span><span class="myModal">Species Glossary</span></td> </tr><tr class="lined"> <td><strong>Applications</strong></td> <td><span title='Western Blot'>WB</span>, <span title='Immunohistochemistry'>IHC</span>, <span title='Knockout Validated'>KO</span></td> </tr><tr class="lined"> <td><strong>Clonality</strong></td> <td><div>Polyclonal</div></td> </tr><tr class="lined"> <td><strong>Host</strong></td> <td><div>Rabbit</div></td> </tr><tr class="lined"> <td><strong>Conjugate</strong></td> <td><div>Unconjugated</div></td> </tr><tr class="lined"> <td><strong>Concentration</strong></td> <td><div>LYOPH</div></td> </tr></table></div><div class="AtlasDSDiv" style="clear:left;"><div class="AtlasDSValidatedText">Validated by: </div>    <span class="AtlasDSSpan"><a href="/5-pillars-validation" class="AtlasDSGenetic" title="Genetic Strategies"> </a><p><a href="/5-pillars-validation" title="Genetic Strategies">Genetic Strategies</a></p></span>    </div> </div></div><div class="grid_6"><div></div> <div class="ds_blue_border equaltop"> <h2 class="blueHead_nopadding">Order Details</h2><ul class="ds_details"><li class="add-to-cart" data-type="full" data-prodname="Human/Mouse/Rat ERK2 Affinity Purified Polyclonal Ab" data-catnum="AF1230" data-catnums="AF1230,AF1230-SP" data-disabled=''><noscript><form autocomplete="off" action="/products/erk2-antibody_af1230" method="post" id="et-add-multiple-to-cart-form" accept-charset="UTF-8"><div><table class="sticky-enabled"> <thead><tr><th>Size</th><th>Price</th> </tr></thead> <tbody> <tr class="odd"><td><div class="atc_size" title="catalog # AF1230-SP">25 ug</div><div data-msg='' class="atc_catnum">AF1230-SP</div></td><td><div style="font-size:11px;"><a class="jump_distributor" href="#jump_distributor">Contact Your<br />Distributor</a></div></td> </tr> <tr class="even"><td colspan="3" class="stock_row">8-10 Business Days</td> </tr> <tr class="odd"><td><div class="atc_size" title="catalog # AF1230">100 ug</div><div data-msg='' class="atc_catnum">AF1230</div></td><td><div style="font-size:11px;"><a class="jump_distributor" href="#jump_distributor">Contact Your<br />Distributor</a></div></td> </tr> <tr class="even"><td colspan="3" class="stock_row">4-6 Business Days</td> </tr> </tbody> </table> <input type="hidden" name="form_build_id" value="form-UQfh8a98G8fUr679GFdxsuhWasi0oYqo92f2BNgBci0" /> <input type="hidden" name="form_id" value="et_add_multiple_to_cart_form" /> </div></form></noscript></li></ul><div id="shipping" class="hidden"></td></tr></div><div class="conj_form_buttons"><div class="list_expand options_closed" data-name="Formulations" data-wrapper-type=".formulations_wrapper"><div class="options_text">View Available Formulations</div> <div class="expander option_closed"></div></div><div class="formulations_wrapper" data-title="ERK2 Antibody [Unconjugated]" data-catnum="AF1230"><table class="scf_table"> <thead> <tr> <th>Catalog# & Formulation</th> <th>Size</th> <th class="col_price">Price</th> <th class="col_cart"></th> </tr> </thead> <tbody><tr class="popup_sku" data-sku="AF1230-SP"> <td> <a href="/products/erk2-antibody_af1230" class="conj_link">Carrier Free</a><br /> <a href="/products/erk2-antibody_af1230" class="cat_link">AF1230-SP</a> </td> <td><div class="popup_size"></div></td> <td class="col_price"><div class="popup_price"></div></td> <td class="col_cart"><a href="/commerce/add-to-cart/427410"><div class="purchase_cart"></div></a></td> </tr> <tr> <td colspan="4" class="row_stock"><div class="popup_stock"></div></td> </tr> <tr class="scf_footer"> <td colspan="4"><div class="expander option_opened"></div></td> </tr> </tbody> </table> <div class="popup_skus" data-skus='["AF1230-SP"]' data-rendered="false"></div></div></div><div id="shipping_statement"><span id="shipping_rate"></span></div><div class="shipping_obt_transfer"><span>Novus Biologicals is part of Bio-Techne</span><br><br><a href="https://www.bio-techne.com/p/antibodies/human-mouse-rat-erk2-antibody_af1230" rel="follow" class="btn btn-primary">Shop this product on bio-techne.com</a></div><br><div class="learn_more"> <a href="/guarantee"> <div class="sprite_noImage" title="Novus Biologicals, part of Bio-Techne, offers 100% Guarantee"></div> <div class="learn_more_text">Novus Biologicals, part of Bio-Techne, offers 100% Guarantee</div></a> </div><span id="conj_form" class="hidden"><tr><td colspan="4"></td></tr></span> </div> </div><div class="clear"></div><div class="grid_10"><div><div id="ds_tabs" style="display:none;"> <div class="ds_tab_active" id="dsTabs_datasheet" data-anchor_desc="datasheet">Datasheet</div> <div class="ds_tab" id="dsTabs_reviews-publications" data-anchor_desc="reviews-publications">Reviews & Publications</div> <div class="ds_tab" id="dsTabs_protocols-faqs" data-anchor_desc="protocols-faqs">Protocols & FAQs</div> <div class="ds_tab" id="dsTabs_supportresearch" data-anchor_desc="supportresearch">Support & Research</div> </div><div class="ds_tab_content equalbottom" id="content_datasheet" > <a id="datasheet"></a><h2 class=greyHead2>ERK2 Antibody [Unconjugated] Summary</h2> <div class="ds_info"> <a id="tab1details"></a> <table class="ds_list wide" style="table-layout:fixed;"> <tr class="lined"> <td><strong>Immunogen</strong></td> <td><div><EM>E. coli</EM>-derived recombinant human ERK2<br />Met1-Ser360<br />Accession # P28482</div></td> </tr><tr class="lined"> <td><strong>Specificity</strong></td> <td><div>Detects human, mouse, and rat ERK2. Also recognizes ERK1 in some cell types at much lower affinity.</div></td> </tr><tr class="lined"> <td><strong>Source</strong></td> <td><div>N/A</div></td> </tr><tr class="lined"> <td><strong>Isotype</strong></td> <td><div>IgG</div></td> </tr><tr class="lined"> <td><strong>Clonality</strong></td> <td><div>Polyclonal</div></td> </tr><tr class="lined"> <td><strong>Host</strong></td> <td><div>Rabbit</div></td> </tr><tr class="lined"> <td><strong>Gene</strong></td> <td><div>MAPK1</div></td> </tr><tr class="lined"> <td><strong>Purity Statement</strong></td> <td><div>Antigen and protein A Affinity-purified</div></td> </tr><tr class="lined"> <td><strong>Innovator's Reward</strong></td> <td><div>Test in a species/application not listed above to receive a full credit towards a future purchase.<br/><div class='text_right'><a class='ds_submit_blue ds_regularPad' href='/support/innovators-reward.html'>Learn about the Innovator's Reward</a></div></div></td> </tr> </table> </div> <h2 class="greyHead2">Applications/Dilutions</h2> <div class="ds_info"> <a id="tab1appsdiluts"></a> <table class="ds_list wide"> <tr class="lined"> <td><strong>Dilutions</strong></td> <td> <ul class="ds_noDotList"><li>Immunohistochemistry 3-15 ug/mL</li><li>Knockout Validated </li><li>Western Blot 0.1 ug/mL</li> </ul> </td> </tr><tr class="lined"> <td><strong>Publications</strong></td> <td> <table> <tr> <td>Read <a href="#PublicationSection"> Publications</a> using<br/> AF1230 in the following applications:<br /><br /> <ul class="information-list"><li class="noIndent"><div class="information-subject"> <a href="#PublicationSection" class="filter_link" data-destination="publication" data-type="application" data-filter="Functional Assay">Functional Assay</a> </div><div class="information-data">1 publication</div></li></ul></td> </tr> </table> </td> </tr> </table> </div> <h2 class="greyHead2">Packaging, Storage & Formulations</h2><div class="ds_info"><table class="ds_list"><tr class="lined"> <td><strong>Storage</strong></td> <td><div><b>Use a manual defrost freezer and avoid repeated freeze-thaw cycles. </b><UL><LI>12 months from date of receipt, -20 to -70 °C as supplied.</LI><LI>1 month, 2 to 8 °C under sterile conditions after reconstitution.</LI><LI>6 months, -20 to -70 °C under sterile conditions after reconstitution.</LI></UL></div></td> </tr><tr class="lined"> <td><strong>Buffer</strong></td> <td><div>Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.</div></td> </tr><tr class="lined"> <td><strong>Preservative</strong></td> <td><div>No Preservative</div></td> </tr><tr class="lined"> <td><strong>Concentration</strong></td> <td><div>LYOPH</div></td> </tr><tr class="lined"> <td><strong>Reconstitution Instructions</strong></td> <td><div>Reconstitute at 0.2 mg/mL in sterile PBS.</div></td> </tr></table></div> <h2 class="greyHead2">Notes</h2><div class="ds_info"> <a id="tab1notes"></a> <div>This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.</div> </div><h2 class="greyHead2">Alternate Names for ERK2 Antibody [Unconjugated]</h2> <div class="ds_info"> <a id="tab1altnames"></a> <div class="information-list"> <ul><li>EC 2.7.11</li><li>EC 2.7.11.24</li><li>ERK</li><li>ERK2</li><li>ERK-2</li><li>ERK2MAP kinase isoform p42</li><li>ERT1</li><li>Extracellular signal-regulated kinase 2</li><li>MAP kinase 1</li><li>MAP kinase 2</li><li>MAPK 1</li><li>MAPK1</li><li>MAPK2</li><li>mitogen-activated protein kinase 1</li><li>Mitogen-activated protein kinase 2</li><li>p38</li><li>p40</li><li>p41</li><li>p41mapk</li><li>p42mapk</li><li>p42-MAPK</li><li>PRKM1</li><li>PRKM1MAPK 2</li><li>PRKM2</li><li>protein tyrosine kinase ERK2</li></ul></div> </div> <h2 class="greyHead2">Background</h2> <div class="ds_info"> <a id="tab1background"></a> <div>ERK1 and ERK2 (also known as MAPK3 and MAPK1) are 44- and 42-kDa Ser/Thr kinases, respectively. They are part of the Ras-Raf-ERK signal transduction cascade often found downstream of growth factor receptor activation. ERK1 and ERK2 were initially isolated and cloned as kinases activated in response to insulin and NGF. They are expressed in most, if not all, mammalian tissues. Dual threonine and tyrosine phosphorylation activate both ERKs, at Thr202/Tyr204 for human ERK1 and Thr185/Tyr187 for human ERK2. Within the range used as an immunogen, human, mouse, and rat ERK1 share 100% amino acid sequence identity.</div> </div> <h2 class="greyHead2">Limitations</h2> <div class="ds_info"> <a id="tab1background"></a> <div>This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are <a href="/support/quality-assurance.html">guaranteed</a> for 1 year from date of receipt.</div> </div><h2 class="greyHead2">Customers Who Viewed This Item Also Viewed...</h2> <a id="custalsoviewed"></a><div id="cav_prev"><div class="sprite_leftArrow"></div></div> <div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout=0 data-cycle-prev="#cav_prev" data-cycle-next="#cav_next" data-cycle-slides="div.ds_cav_group" id="ds_customer_also_viewed"><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NBP2-22203"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/erk1-antibody-1e5_nbp2-22203" style="overflow: hidden; width: 280px;" title="ERK1 Antibody (1E5) - BSA Free">ERK1 Antibody<br> (1E5) - BSA Free</a>    </div><div class="sub-header">NBP2-22203</div> <div class="images_customers"><div id="ss_prev_cav0" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-0" data-cycle-prev="#ss_prev_cav0" data-cycle-next="#ss_next_cav0" data-cycle-slides="div.big_thumb_cust_container" data-index=0 ><div class="big_thumb_cust_container"> <a href="/products/erk1-antibody-1e5_nbp2-22203" title="Flow Cytometry: ERK1 Antibody (1E5) [NBP2-22203] - Flow cytometric analysis of Hela cells using ERK1 mouse mAb (blue) and negative control (red)." class="cust_viewed_0" > <img loading="lazy" src="https://images.novusbio.com/images/ERK1-Antibody-1E5-Flow-Cytometry-NBP2-22203-img0001.jpg" alt="Flow Cytometry ERK1 Antibody (1E5) - BSA Free" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/erk1-antibody-1e5_nbp2-22203" title="Immunocytochemistry/Immunofluorescence: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of NIH/3T3 cells using ERK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. 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A specific band was detected for p38 gamma at approximately 48&amp;nbsp;kDa (as indicated) using 2&amp;nbsp;µg/mL of Rabbit Anti-Human/Mouse/Rat p38 gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1347). This experiment was conducted&amp;nbsp;under&amp;nbsp;reducing&amp;nbsp;conditions and using the 12-230 kDa separation system. " class="cust_viewed_1" > <img loading="lazy" src="https://images.novusbio.com/images2/p38_gamma_AF1347_Simple_Western_16621.jpg" alt="Simple Western p38 gamma/SAPK3 Antibody [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/p38-gamma-sapk3-antibody_af1347" title="Western blot shows lysates of PC-12 rat adrenal pheochromocytoma cell line, C6 rat glioma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.2 µg/mL Rabbit Anti-Human/Mouse/Rat p38 gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1347) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for p38 gamma was detected at approximately 40 kDa (as indicated). 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PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse/Rat JNK1 Monoclonal Antibody (Catalog # MAB17761) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for JNK1 was detected at approximately 46 kDa and 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4." class="cust_viewed_2" > <img loading="lazy" src="https://images.novusbio.com/images2/JNK1_MAB17761_Western_Blot_5991.jpg" alt="Western Blot JNK1 Antibody (228601) [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/jnk1-antibody-228601_mab17761" title="Western blot shows recombinant human JNK1, JNK2, and JNK3 (1 ng/lane). PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse/Rat JNK1 Monoclonal Antibody (Catalog # MAB17761) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). 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View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="cust_viewed_2" > <img loading="lazy" src="https://images.novusbio.com/images2/JNK1_MAB17761_Immunocytochemistry__Immunofluorescence_20184.jpg" alt="Immunocytochemistry JNK1 Antibody (228601) [Unconjugated]" class="big_thumb" /> </a> </div></div><div id="ss_next_cav2" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-2" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/jnk1-antibody-228601_mab17761#ReviewsSection"> <div class="sprite_4Stars"></div> (1 Review</a>) </div> <div class="tiny"> <a href="/products/jnk1-antibody-228601_mab17761#PublicationSection"> <div class="sprite_pubBook"></div> (3 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu, Mu, Rt</div><div class="tiny"><strong>Applications:</strong> ICC, WB</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="AF887"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/akt-antibody_af887" style="overflow: hidden; width: 280px;" title="AKT [p Ser473] Antibody [Unconjugated] - Pan Specific">AKT [p Ser473]<br> Antibody [Unconjugate ...</a>    </div><div class="sub-header">AF887</div> <div class="images_customers"><div id="ss_prev_cav3" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-3" data-cycle-prev="#ss_prev_cav3" data-cycle-next="#ss_next_cav3" data-cycle-slides="div.big_thumb_cust_container" data-index=3 ><div class="big_thumb_cust_container"> <a href="/products/akt-antibody_af887" title="Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 100 ng/mL Human PDGF (120-HD) for 15 minutes and MCF-7 human breast cancer cell line untreated or treated with 100 ng/mL Recombinant Human IGF-1 (291-G1) for 15 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF887), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for Phospho-Akt (S473) Pan Specific at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="cust_viewed_3" > <img loading="lazy" src="https://images.novusbio.com/images2/Akt3_AF887_Western_Blot_5930.jpg" alt="Western Blot AKT [p Ser473] Antibody [Unconjugated] - Pan Specific" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/akt-antibody_af887" title="Simple Western lane view shows lysates of MCF-7 human breast cancer cell line and A549 human lung carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IGF-I (291-G1) for 15 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-Akt (S473) at approximately 60 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF887). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="cust_viewed_3" > <img loading="lazy" src="https://images.novusbio.com/images2/16332.jpg" alt="Simple Western AKT [p Ser473] Antibody [Unconjugated] - Pan Specific" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/akt-antibody_af887" title="Serum-starved MCF-7 human breast cancer cells were unstimulated (blue open histogram) or activated with 100 ng/mL Recombinant human IGF-1 (Catalog # 291-G1, dark orange filled histogram) for 15 minutes, then stained with Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF887) followed by PE-conjugated Anti-Rabbit IgG Secondary Antibody (F0110). 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PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-c-Jun (S63) Monoclonal Antibody (Catalog # MAB8930) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-c-Jun (S63) at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="cust_viewed_4" > <img loading="lazy" src="https://images.novusbio.com/images2/cJun_MAB8930_Western_Blot_18923.jpg" alt="Western Blot c-jun [p Ser63] Antibody (1018Y) [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/c-jun-antibody-1018y_mab8930" title="c-Jun phosphorylated at S63 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line treated with Anisomycin using Rabbit Anti-Human Phospho-c-Jun (S63) Monoclonal Antibody (Catalog # MAB8930) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights&amp;#8482; 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="cust_viewed_4" > <img loading="lazy" src="https://images.novusbio.com/images2/cJun_MAB8930_Immunocytochemistry__Immunofluorescence_18945.jpg" alt="Immunocytochemistry c-jun [p Ser63] Antibody (1018Y) [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/c-jun-antibody-1018y_mab8930" title="Simple Western lane view shows lysates of HEK293T human embryonic kidney cell line untreated (-) or treated (+) with 20 mJ/cm2 ultraviolet light (UV)&amp;nbsp;with a&amp;nbsp;30 minute recovery, loaded at 0.2&amp;nbsp;mg/mL. A specific band was detected for Phospho-c‑Jun&amp;nbsp;(S63)&amp;nbsp;at approximately 49&amp;nbsp;kDa (as indicated) using&amp;nbsp;5 μg/mL&amp;nbsp;of Rabbit Anti-Human Phospho-c‑Jun (S63) Monoclonal Antibody (Catalog # MAB8930) . This experiment was conducted under reducing&amp;nbsp;conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody." class="cust_viewed_4" > <img loading="lazy" src="https://images.novusbio.com/images2/cJun_MAB8930_Simple_Western_19175.jpg" alt="Simple Western c-jun [p Ser63] Antibody (1018Y) [Unconjugated]" class="big_thumb" /> </a> </div></div><div id="ss_next_cav4" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-4" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/c-jun-antibody-1018y_mab8930#ReviewsSection"> <div class="sprite_5Stars"></div> (1 Review</a>) </div> <div class="tiny"> <a href="/products/c-jun-antibody-1018y_mab8930#PublicationSection"> <div class="sprite_pubBook"></div> (3 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu</div><div class="tiny"><strong>Applications:</strong> ICC, Simple Western, WB</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NBP2-13304"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/sgsm3-antibody_nbp2-13304" style="overflow: hidden; width: 280px;" title="SGSM3 Antibody">SGSM3 Antibody</a>    </div><div class="sub-header">NBP2-13304</div> <div class="images_customers"><div id="ss_prev_cav5" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-5" data-cycle-prev="#ss_prev_cav5" data-cycle-next="#ss_next_cav5" data-cycle-slides="div.big_thumb_cust_container" data-index=5 ><div class="big_thumb_cust_container"> <a href="/products/sgsm3-antibody_nbp2-13304" title="Immunohistochemistry-Paraffin: SGSM3 Antibody [NBP2-13304] - Staining of human stomach, lower shows strong granular cytoplasmic positivity in glandular cells." class="cust_viewed_5" > <img loading="lazy" src="https://images.novusbio.com/images/SGSM3-Antibody-Immunohistochemistry-Paraffin-NBP2-13304-img0001.jpg" alt="Immunohistochemistry-Paraffin SGSM3 Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/sgsm3-antibody_nbp2-13304" title="Immunocytochemistry/Immunofluorescence: SGSM3 Antibody [NBP2-13304] - Immunofluorescent staining of human cell line MCF7 shows localization to the Golgi apparatus." class="cust_viewed_5" > <img loading="lazy" src="https://images.novusbio.com/images/SGSM3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-13304-img0005.jpg" alt="Immunocytochemistry/ Immunofluorescence SGSM3 Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/sgsm3-antibody_nbp2-13304" title="Western Blot: SGSM3 Antibody [NBP2-13304] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4" class="cust_viewed_5" > <img loading="lazy" src="https://images.novusbio.com/images/SGSM3-Antibody-Western-Blot-NBP2-13304-img0004.jpg" alt="Western Blot SGSM3 Antibody" class="big_thumb" /> </a> </div></div><div id="ss_next_cav5" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-5" class="cycle-circle-pager"></div></div> <div class="ds_info"><div class="tiny"><strong>Species:</strong> Hu</div><div class="tiny"><strong>Applications:</strong> ICC/IF, IHC, IHC-P, WB</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="210-TA"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/recombinant-human-tnf-alpha-protein_210-ta" style="overflow: hidden; width: 280px;" title="TNF-alpha [Unconjugated]">TNF-alpha<br> [Unconjugated]</a>    </div><div class="sub-header">210-TA</div> <div class="images_customers"><div id="ss_prev_cav6" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-6" data-cycle-prev="#ss_prev_cav6" data-cycle-next="#ss_next_cav6" data-cycle-slides="div.big_thumb_cust_container" data-index=6 ><div class="big_thumb_cust_container"> <a href="/products/recombinant-human-tnf-alpha-protein_210-ta" title="1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa." class="cust_viewed_6" > <img loading="lazy" src="https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg" alt="SDS-PAGE TNF-alpha [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/recombinant-human-tnf-alpha-protein_210-ta" title="Recombinant Human TNF-alpha (Catalog # 210‑TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 25‑100 pg/mL." class="cust_viewed_6" > <img loading="lazy" src="https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg" alt="Bioactivity TNF-alpha [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/recombinant-human-tnf-alpha-protein_210-ta" title="Recombinant Human TNF-alpha (Catalog # 210-TA) has a molecular weight (MW) of 53.1 kDa as analyzed by SEC-MALS, suggesting that this protein is a homotrimer. MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation)." class="cust_viewed_6" > <img loading="lazy" src="https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg" alt="SEC-MALS TNF-alpha [Unconjugated]" class="big_thumb" /> </a> </div></div><div id="ss_next_cav6" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-6" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/recombinant-human-tnf-alpha-protein_210-ta#ReviewsSection"> <div class="sprite_5Stars"></div> (3 Reviews</a>) </div> <div class="tiny"> <a href="/products/recombinant-human-tnf-alpha-protein_210-ta#PublicationSection"> <div class="sprite_pubBook"></div> (811 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu</div><div class="tiny"><strong>Applications:</strong> BA</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NBP1-47833"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/mek1-antibody-oti1f5_nbp1-47833" style="overflow: hidden; width: 280px;" title="MEK1 Antibody (OTI1F5)">MEK1 Antibody<br> (OTI1F5)</a>    </div><div class="sub-header">NBP1-47833</div> <div class="images_customers"><div id="ss_prev_cav7" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-7" data-cycle-prev="#ss_prev_cav7" data-cycle-next="#ss_next_cav7" data-cycle-slides="div.big_thumb_cust_container" data-index=7 ><div class="big_thumb_cust_container"> <a href="/products/mek1-antibody-oti1f5_nbp1-47833" title="Western Blot: MEK1 Antibody (1F5) [NBP1-47833] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MEK1(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MEK1." class="cust_viewed_7" > <img loading="lazy" src="https://images.novusbio.com/images/MEK1-Antibody-1F5-Western-Blot-NBP1-47833-img0001.jpg" alt="Western Blot MEK1 Antibody (OTI1F5)" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/mek1-antibody-oti1f5_nbp1-47833" title="Immunohistochemistry-Paraffin: MEK1 Antibody (1F5) [NBP1-47833] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-MEK1 mouse monoclonal antibody." class="cust_viewed_7" > <img loading="lazy" src="https://images.novusbio.com/images/MEK1-Antibody-1F5-Immunohistochemistry-Paraffin-NBP1-47833-img0002.jpg" alt="Immunohistochemistry-Paraffin MEK1 Antibody (OTI1F5)" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/mek1-antibody-oti1f5_nbp1-47833" title="Immunohistochemistry-Paraffin: MEK1 Antibody (1F5) [NBP1-47833] - Staining of paraffin-embedded Carcinoma of bladder tissue using anti-MEK1 mouse monoclonal antibody." class="cust_viewed_7" > <img loading="lazy" src="https://images.novusbio.com/images/MEK1-Antibody-1F5-Immunohistochemistry-Paraffin-NBP1-47833-img0003.jpg" alt="Immunohistochemistry-Paraffin MEK1 Antibody (OTI1F5)" class="big_thumb" /> </a> </div></div><div id="ss_next_cav7" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-7" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/mek1-antibody-oti1f5_nbp1-47833#ReviewsSection"> <div class="sprite_5Stars"></div> (1 Review</a>) </div> <div class="tiny"> <a href="/products/mek1-antibody-oti1f5_nbp1-47833#PublicationSection"> <div class="sprite_pubBook"></div> (1 Publication</a>) </div><div class="tiny"><strong>Species:</strong> Hu, Mu, Rt</div><div class="tiny"><strong>Applications:</strong> ICC/IF, IHC, IHC-P, WB</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="AF231"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/egfr-antibody_af231" style="overflow: hidden; width: 280px;" title="EGFR Antibody [Unconjugated]">EGFR Antibody<br> [Unconjugated]</a>    </div><div class="sub-header">AF231</div> <div class="images_customers"><div id="ss_prev_cav8" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-8" data-cycle-prev="#ss_prev_cav8" data-cycle-next="#ss_next_cav8" data-cycle-slides="div.big_thumb_cust_container" data-index=8 ><div class="big_thumb_cust_container"> <a href="/products/egfr-antibody_af231" title=" EGFR was detected in immersion fixed&amp;nbsp;frozen sections of human&amp;nbsp;skin using Goat Anti-Human EGFR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF231) at 1&amp;nbsp;µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte&amp;#8482; HRP Polymer Antibody (Catalog&amp;nbsp;# VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents. " class="cust_viewed_8" > <img loading="lazy" src="https://images.novusbio.com/images2/EGF_R_AF231_Immunohistochemistry_20892.jpg" alt="Immunohistochemistry EGFR Antibody [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/egfr-antibody_af231" title="EGFR was detected in immersion fixed A431 human epithelial carcinoma cell line using Goat Anti-Human EGFR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF231) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights&amp;#8482; 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="cust_viewed_8" > <img loading="lazy" src="https://images.novusbio.com/images2/EGF_R_AF231_Immunocytochemistry__Immunofluorescence_21143.jpg" alt="Immunocytochemistry EGFR Antibody [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/egfr-antibody_af231" title="Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and MDA-MB-231 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human EGFR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF231) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for EGFR at approximately 175 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="cust_viewed_8" > <img loading="lazy" src="https://images.novusbio.com/images2/EGF_R_AF231_Western_Blot_19925.jpg" alt="Western Blot EGFR Antibody [Unconjugated]" class="big_thumb" /> </a> </div></div><div id="ss_next_cav8" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-8" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/egfr-antibody_af231#PublicationSection"> <div class="sprite_pubBook"></div> (30 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu</div><div class="tiny"><strong>Applications:</strong> CyTOF-ready, Dual ISH-IHC, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, ICC, IHC, IP, Simple Western, WB</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="H00005706-M02"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/psmc6-antibody-2c4_h00005706-m02" style="overflow: hidden; width: 280px;" title="PSMC6 Antibody (2C4)">PSMC6 Antibody<br> (2C4)</a>    </div><div class="sub-header">H00005706-M02</div> <div class="images_customers"><div id="ss_prev_cav9" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-9" data-cycle-prev="#ss_prev_cav9" data-cycle-next="#ss_next_cav9" data-cycle-slides="div.big_thumb_cust_container" data-index=9 ><div class="big_thumb_cust_container"> <a href="/products/psmc6-antibody-2c4_h00005706-m02" title="Western Blot: PSMC6 Antibody (2C4) [H00005706-M02] - Analysis of PSMC6 expression in transfected 293T cell line by PSMC6 monoclonal antibody (M02), clone 2C4.Lane 1: PSMC6 transfected lysate(44.2 KDa).Lane 2: Non-transfected lysate." class="cust_viewed_9" > <img loading="lazy" src="https://images.novusbio.com/images/PSMC6-Antibody-2C4-Western-Blot-H00005706-M02-img0013.jpg" alt="Western Blot PSMC6 Antibody (2C4)" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/psmc6-antibody-2c4_h00005706-m02" title="Immunocytochemistry/Immunofluorescence: PSMC6 Antibody (2C4) [H00005706-M02] - Analysis of monoclonal antibody to PSMC6 on HeLa cell. Antibody concentration 10 ug/ml." class="cust_viewed_9" > <img loading="lazy" src="https://images.novusbio.com/images/PSMC6-Antibody-2C4-Immunocytochemistry-Immunofluorescence-H00005706-M02-img0008.jpg" alt="Immunocytochemistry/ Immunofluorescence PSMC6 Antibody (2C4)" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/psmc6-antibody-2c4_h00005706-m02" title="Western Blot: PSMC6 Antibody (2C4) [H00005706-M02] - Analysis of PSMC6 over-expressed 293 cell line, cotransfected with PSMC6 Validated Chimera RNAi ( Cat # H00005706-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PSMC6 monoclonal antibody (M02), clone 2C4 (Cat # H00005706-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control." class="cust_viewed_9" > <img loading="lazy" src="https://images.novusbio.com/images/PSMC6-Antibody-2C4-Western-Blot-H00005706-M02-img0009.jpg" alt="Western Blot PSMC6 Antibody (2C4)" class="big_thumb" /> </a> </div></div><div id="ss_next_cav9" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-9" class="cycle-circle-pager"></div></div> <div class="ds_info"><div class="tiny"><strong>Species:</strong> Hu, Mu, Rt</div><div class="tiny"><strong>Applications:</strong> ELISA, ICC/IF, KD, WB</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NBP2-50037"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/c-fos-antibody-2h2_nbp2-50037" style="overflow: hidden; width: 280px;" title="c-Fos Antibody (2H2)">c-Fos Antibody<br> (2H2)</a>    </div><div class="sub-header">NBP2-50037</div> <div class="images_customers"><div id="ss_prev_cav10" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-10" data-cycle-prev="#ss_prev_cav10" data-cycle-next="#ss_next_cav10" data-cycle-slides="div.big_thumb_cust_container" data-index=10 ><div class="big_thumb_cust_container"> <a href="/products/c-fos-antibody-2h2_nbp2-50037" title="Knockout Validated: c-Fos Antibody (2H2) [NBP2-50037] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and c-Fox knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Mouse Anti-Human c-Fox Monoclonal Antibody (Catalog # NBP2-50037) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for c-Fox at approximately 52 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions." class="cust_viewed_10" > <img loading="lazy" src="https://images.novusbio.com/images/c-Fos-Antibody-2H2-Knockout-Validated-NBP2-50037-img0005.jpg" alt="Knockout Validated c-Fos Antibody (2H2)" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/c-fos-antibody-2h2_nbp2-50037" title="Immunocytochemistry/Immunofluorescence: c-Fos Antibody (2H2) [NBP2-50037] - Section of rat hippocampus stained with mouse monoclonal antibody to c-FOS NBP2-50037 in red and counterstained with rabbit polyclonal antibody to FOX3/NeuN. DAPI reveals nuclei of neurons and glia in blue. The hippocampal neurons stain green for FOX3/NeuN and a few also are expressing c-FOS, and so appear orange. These cells were spontaneously active at the time the animal was sacrificed." class="cust_viewed_10" > <img loading="lazy" src="https://images.novusbio.com/images/c-Fos-Antibody-2H2-Immunocytochemistry-Immunofluorescence-NBP2-50037-img0006.jpg" alt="Immunocytochemistry/ Immunofluorescence c-Fos Antibody (2H2)" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/c-fos-antibody-2h2_nbp2-50037" title="Immunocytochemistry/Immunofluorescence: c-Fos Antibody (2H2) [NBP2-50037] - Left: NBP2-50037 staining (green) in HeLa cells, which were treated with serum-starvation for 36 hrs, followed by 2 hrs 20% FBS stimulation (bottom), or PBS treatment (top). Green c-Fos staining only localizes in the nuclei of stimulated cells, but not in un-stimulated cells. Cells are counter-stained with chicken pAb against Vimentin (NB300-223, red). Blue shows DAPI staining of nucleus. Middle: Mouse brain section (45 uM; fixed by transcardial perfusion with 4% PFA) labeled with NBP2-50037 using a standard HRP-DAB staining technique. Cells expressing c-Fos show dark color in nucleus. Right: Mouse cortical section labeled with NBP2-50037 (red) and rabbit polyclonal anti-NeuN (NBP1-92716, green) using IF confocal. Neurons positive for c-Fos and RBFOX3/NeuN appear to be yellow. Inset shows an enlarged image of NBP2-50037 staining. Nuclei are labeled with Dapi (blue)." class="cust_viewed_10" > <img loading="lazy" src="https://images.novusbio.com/images/c-Fos-Antibody-2H2-Immunocytochemistry-Immunofluorescence-NBP2-50037-img0001.jpg" alt="Immunocytochemistry/ Immunofluorescence c-Fos Antibody (2H2)" class="big_thumb" /> </a> </div></div><div id="ss_next_cav10" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-10" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/c-fos-antibody-2h2_nbp2-50037#ReviewsSection"> <div class="sprite_4Stars"></div> (1 Review</a>) </div> <div class="tiny"> <a href="/products/c-fos-antibody-2h2_nbp2-50037#PublicationSection"> <div class="sprite_pubBook"></div> (20 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu, Mu, Rt</div><div class="tiny"><strong>Applications:</strong> ICC/IF, IHC, KO, WB</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="236-EG"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/recombinant-human-egf-protein-cf_236-eg" style="overflow: hidden; width: 280px;" title="EGF [Unconjugated]">EGF [Unconjugated]</a>    </div><div class="sub-header">236-EG</div> <div class="images_customers"><div id="ss_prev_cav11" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-11" data-cycle-prev="#ss_prev_cav11" data-cycle-next="#ss_next_cav11" data-cycle-slides="div.big_thumb_cust_container" data-index=11 ><div class="big_thumb_cust_container"> <a href="/products/recombinant-human-egf-protein-cf_236-eg" title="1 µg/lane of Recombinant Human EGF was resolved with SDS-PAGE and visualized by silver staining under reducing (R) conditions, showing a single band at 6 kDa." class="cust_viewed_11" > <img loading="lazy" src="https://images.novusbio.com/images2/EGF_236-EG_234.jpg" alt="SDS-PAGE EGF [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/recombinant-human-egf-protein-cf_236-eg" title="Recombinant Human EGF (Catalog # 236‑EG) stimulates cell proliferation of the Balb/3T3 mouse embryonic fibroblast cell line. The ED50 for this effect is 20‑100 pg/mL." class="cust_viewed_11" > <img loading="lazy" src="https://images.novusbio.com/images2/EGF_236EG_1570.jpg" alt="Bioactivity EGF [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/recombinant-human-egf-protein-cf_236-eg" title="iPSC-derived human intestinal organoids were cultured using Cultrex&amp;#8482; UltiMatrix RGF Basement Membrane Extract (BME001-05) and intestinal organoid culture medium, which includes Recombinant Human EGF (Catalog # 236-EG), Recombinant Human Noggin (6057-NG), Recombinant Human R-Spondin 1 (4645-RS), and Recombinant Human Wnt-3a (5036-WN), along with the other reagents listed in the intestinal organoid culture medium recipe in the human intestinal organoid culture protocol. (A) Human intestinal organoids were stained using a Rat Anti-Human/Mouse/Rat Vimentin Monoclonal Antibody (MAB2105; green) and a Goat Anti-Human/Mouse Desmin Antigen Affinity-purified Polyclonal Antibody (AF3844; red) to visualize myofibroblast cells and counterstained with DAPI (5748; blue). (B) Human intestinal organoids were stained using a Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (AF748; green) and a Mouse Anti-Human MUC2 Monoclonal Antibody (Novus Biologicals, Catalog # NBP2-44431; red) and counterstained with DAPI (5748; blue)." class="cust_viewed_11" > <img loading="lazy" src="https://images.novusbio.com/images/236-eg_recombinant-human-egf-protein-cf-bioactivity-25202394053.jpg" alt="Cell Culture EGF [Unconjugated]" class="big_thumb" /> </a> </div></div><div id="ss_next_cav11" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-11" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/recombinant-human-egf-protein-cf_236-eg#PublicationSection"> <div class="sprite_pubBook"></div> (836 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu</div><div class="tiny"><strong>Applications:</strong> BA</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="M6000B"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/mouse-il-6-quantikine-elisa-kit-2-plate_m6000b" style="overflow: hidden; width: 280px;" title="IL-6 [HRP]">IL-6 [HRP]</a>    </div><div class="sub-header">M6000B</div> <div class="images_customers"><div id="ss_prev_cav12" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-12" data-cycle-prev="#ss_prev_cav12" data-cycle-next="#ss_next_cav12" data-cycle-slides="div.big_thumb_cust_container" data-index=12 ><div class="big_thumb_cust_container"> <a href="/products/mouse-il-6-quantikine-elisa-kit-2-plate_m6000b" title="" class="cust_viewed_12" > <img loading="lazy" src="https://images.novusbio.com/images2/DATA_IL6_M6000_ELISA_936.jpg" alt="N/A IL-6 [HRP]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/mouse-il-6-quantikine-elisa-kit-2-plate_m6000b" title="" class="cust_viewed_12" > <img loading="lazy" src="https://images.novusbio.com/images2/IL-6_M6000_ELISA_415.jpg" alt="N/A IL-6 [HRP]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/mouse-il-6-quantikine-elisa-kit-2-plate_m6000b" title="Increased production of proinflammatory cytokines and VEGF in the plasma and chondrocytes in high fat diet (HFD)-feeding mice.(a~d) levels of plasma IL-6 (a), TNF-alpha (b), IL-1 beta (c) and VEGF (d) in the C57BL/6 male mice fed with a normal diet (ND) or HFD for 8 weeks. (n = 5, **P &lt; 0.01). (e~h) Relative mRNA expression of IL-6 (e), TNF-alpha (f), IL-1 beta (g) and VEGF (h) in the chondrocytes isolated from the 8-week ND or HFD-feeding mice. (n = 5, **P &lt; 0.01). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26271607), licensed under a CC-BY license. Not internally tested by R&amp;D Systems." class="cust_viewed_12" > <img loading="lazy" src="https://images.novusbio.com/images/m6000b_mouse-il-6-quantikine-elisa-kit-44202415433118.jpg" alt="N/A IL-6 [HRP]" class="big_thumb" /> </a> </div></div><div id="ss_next_cav12" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-12" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/mouse-il-6-quantikine-elisa-kit-2-plate_m6000b#PublicationSection"> <div class="sprite_pubBook"></div> (661 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Mu</div><div class="tiny"><strong>Applications:</strong> ELISA</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NB100-56583"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/nfkb-p105-p50-antibody-2j10d7_nb100-56583" style="overflow: hidden; width: 280px;" title="NFkB p105/p50 Antibody (2J10D7) - BSA Free">NFkB p105/p50<br> Antibody (2J10D7) - B ...</a>    </div><div class="sub-header">NB100-56583</div> <div class="images_customers"><div id="ss_prev_cav13" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-13" data-cycle-prev="#ss_prev_cav13" data-cycle-next="#ss_next_cav13" data-cycle-slides="div.big_thumb_cust_container" data-index=13 ><div class="big_thumb_cust_container"> <a href="/products/nfkb-p105-p50-antibody-2j10d7_nb100-56583" title="Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - An intracellular stain was performed on Jurkat cells with NFkB p105/p50 Antibody (2J10D7) NB100-56583C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes. Both antibodies were conjugated to Dylight 650." class="cust_viewed_13" > <img loading="lazy" src="https://images.novusbio.com/images/NFkB-p105-p50-Antibody-2J10D7-Flow-Cytometry-NB100-56583-img0005.jpg" alt="Flow Cytometry NFkB p105/p50 Antibody (2J10D7) - BSA Free" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/nfkb-p105-p50-antibody-2j10d7_nb100-56583" title="Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - An intracellular stain was performed on HeLa cells with NFkB p105/p50 Antibody (2J10D7) NB100-56583C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes. Both antibodies were conjugated to Dylight 650." class="cust_viewed_13" > <img loading="lazy" src="https://images.novusbio.com/images/NFkB-p105-p50-Antibody-2J10D7-Flow-Cytometry-NB100-56583-img0006.jpg" alt="Flow Cytometry NFkB p105/p50 Antibody (2J10D7) - BSA Free" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/nfkb-p105-p50-antibody-2j10d7_nb100-56583" title="Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - An intracellular stain was performed on U2-OS cells with NFkB p105/p50 [2J10D7] Antibody NB100-56583C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650." class="cust_viewed_13" > <img loading="lazy" src="https://images.novusbio.com/images/NFkB-p105-p50-Antibody-2J10D7-Flow-Cytometry-NB100-56583-img0007.jpg" alt="Flow Cytometry NFkB p105/p50 Antibody (2J10D7) - BSA Free" class="big_thumb" /> </a> </div></div><div id="ss_next_cav13" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-13" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/nfkb-p105-p50-antibody-2j10d7_nb100-56583#ReviewsSection"> <div class="sprite_5Stars"></div> (1 Review</a>) </div> <div class="tiny"> <a href="/products/nfkb-p105-p50-antibody-2j10d7_nb100-56583#PublicationSection"> <div class="sprite_pubBook"></div> (5 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu, Rb, Rt</div><div class="tiny"><strong>Applications:</strong> Flow, IHC, IHC-P, KO, WB</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="MAB4540"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/raf-1-antibody-563002_mab4540" style="overflow: hidden; width: 280px;" title="Raf-1 Antibody (563002) [Unconjugated]">Raf-1 Antibody<br> (563002) [Unconjugate</a>    </div><div class="sub-header">MAB4540</div> <div class="images_customers"><div id="ss_prev_cav14" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-14" data-cycle-prev="#ss_prev_cav14" data-cycle-next="#ss_next_cav14" data-cycle-slides="div.big_thumb_cust_container" data-index=14 ><div class="big_thumb_cust_container"> <a href="/products/raf-1-antibody-563002_mab4540" title="Western blot shows lysates of MCF-7 human breast cancer cell line, HepG2 human hepatocellular carcinoma cell line, A431 human epithelial carcinoma cell line, L-929 mouse fibroblast cell line, and NRK rat normal kidney cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat Raf-1 Monoclonal Antibody (Catalog # MAB4540) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Raf-1 at approximately 74 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="cust_viewed_14" > <img loading="lazy" src="https://images.novusbio.com/images2/Raf-1_MAB4540_Western_Blot_9426.jpg" alt="Western Blot Raf-1 Antibody (563002) [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/raf-1-antibody-563002_mab4540" title="Raf-1 was detected in immersion fixed paraffin-embedded sections of human liver array using Mouse Anti-Human/Mouse/Rat Raf-1 Monoclonal Antibody (Catalog # MAB4540) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell &amp; Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="cust_viewed_14" > <img loading="lazy" src="https://images.novusbio.com/images2/Raf-1_MAB4540_Immunohistochemistry_9891.jpg" alt="Immunohistochemistry Raf-1 Antibody (563002) [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/raf-1-antibody-563002_mab4540" title="Simple Western lane view shows lysates of MCF&amp;#x2011;7 human breast cancer cell line, loaded at 0.2&amp;nbsp;mg/mL. A specific band was detected for Raf&amp;#x2011;1 at approximately 75&amp;nbsp;kDa (as indicated) using 10&amp;nbsp;µg/mL of Mouse Anti-Human/Mouse/Rat Raf&amp;#x2011;1 Monoclonal Antibody (Catalog # MAB4540) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="cust_viewed_14" > <img loading="lazy" src="https://images.novusbio.com/images2/mab4540_human-mouse-rat-raf-1-mab-clone-563002-simple-western-158202212919.jpg" alt="Simple Western Raf-1 Antibody (563002) [Unconjugated]" class="big_thumb" /> </a> </div></div><div id="ss_next_cav14" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-14" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/raf-1-antibody-563002_mab4540#PublicationSection"> <div class="sprite_pubBook"></div> (2 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu, Mu, Rt</div><div class="tiny"><strong>Applications:</strong> IHC, Simple Western, WB</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NBP2-19804"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/pik3ca-antibody_nbp2-19804" style="overflow: hidden; width: 280px;" title="PIK3CA Antibody">PIK3CA Antibody</a>    </div><div class="sub-header">NBP2-19804</div> <div class="images_customers"><div id="ss_prev_cav15" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-15" data-cycle-prev="#ss_prev_cav15" data-cycle-next="#ss_next_cav15" data-cycle-slides="div.big_thumb_cust_container" data-index=15 ><div class="big_thumb_cust_container"> <a href="/products/pik3ca-antibody_nbp2-19804" title="Western Blot: PIK3CA Antibody [NBP2-19804] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with PIK3CA antibody (NBP2-19804) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced." class="cust_viewed_15" > <img loading="lazy" src="https://images.novusbio.com/images/nbp2-19804_rabbit-polyclonal-pik3ca-antibody-910202420134031.jpg" alt="Western Blot PIK3CA Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/pik3ca-antibody_nbp2-19804" title="Immunohistochemistry-Paraffin: PIK3CA Antibody [NBP2-19804] - Human Breast Cancer, using PI3 kinase p110 alpha antibody. Antigen Retrieval: Trilogy&amp;#8482; (EDTA based, pH 8.0) buffer, 15min. " class="cust_viewed_15" > <img loading="lazy" src="https://images.novusbio.com/images/PIK3CA-Antibody-Immunohistochemistry-Paraffin-NBP2-19804-img0004.jpg" alt="Immunohistochemistry-Paraffin PIK3CA Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/pik3ca-antibody_nbp2-19804" title="Western Blot: PIK3CA Antibody [NBP2-19804] - PIK3CA Antibody Sample (50 ug of whole cell lysate) A: C2C12 B: C2C12+AEA10mM NBP2-19804 diluted at 1:1000." class="cust_viewed_15" > <img loading="lazy" src="https://images.novusbio.com/images/PIK3CA-Antibody-Western-Blot-NBP2-19804-img0006.jpg" alt="Western Blot PIK3CA Antibody" class="big_thumb" /> </a> </div></div><div id="ss_next_cav15" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-15" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/pik3ca-antibody_nbp2-19804#PublicationSection"> <div class="sprite_pubBook"></div> (4 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu, Mu</div><div class="tiny"><strong>Applications:</strong> ICC/IF, IHC, IHC-P, WB</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NBP2-15071"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/pi-3-kinase-p110-gamma-antibody_nbp2-15071" style="overflow: hidden; width: 280px;" title="PI 3-Kinase p110 gamma Antibody">PI 3-Kinase p110<br> gamma Antibody</a>    </div><div class="sub-header">NBP2-15071</div> <div class="images_customers"><div id="ss_prev_cav16" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-16" data-cycle-prev="#ss_prev_cav16" data-cycle-next="#ss_next_cav16" data-cycle-slides="div.big_thumb_cust_container" data-index=16 ><div class="big_thumb_cust_container"> <a href="/products/pi-3-kinase-p110-gamma-antibody_nbp2-15071" title="Western Blot: PI 3-Kinase p110 gamma Antibody [NBP2-15071] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p110 gamma antibody [C3], C-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody." class="cust_viewed_16" > <img loading="lazy" src="https://images.novusbio.com/images/PI-3-Kinase-p110-gamma-Antibody-Western-Blot-NBP2-15071-img0008.jpg" alt="Western Blot PI 3-Kinase p110 gamma Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/pi-3-kinase-p110-gamma-antibody_nbp2-15071" title="Western Blot: PI 3-Kinase p110 gamma Antibody [NBP2-15071] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PI 3-Kinase p110 gamma antibody [C3], C-term (NBP2-15071) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody." class="cust_viewed_16" > <img loading="lazy" src="https://images.novusbio.com/images/nbp2-15071_rabbit-polyclonal-pi-3-kinase-p110-gamma-antibody-91020242063361.jpg" alt="Western Blot PI 3-Kinase p110 gamma Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/pi-3-kinase-p110-gamma-antibody_nbp2-15071" title="Immunohistochemistry-Paraffin: PI 3-Kinase p110 gamma Antibody [NBP2-15071] - Human Breast Cancer, using PI3K gamma antibody. Antigen Retrieval: Trilogy&amp;#8482; (EDTA based, pH 8.0) buffer, 15min" class="cust_viewed_16" > <img loading="lazy" src="https://images.novusbio.com/images/PI-3-Kinase-p110-gamma-Antibody-Immunohistochemistry-Paraffin-NBP2-15071-img0003.jpg" alt="Immunohistochemistry-Paraffin PI 3-Kinase p110 gamma Antibody" class="big_thumb" /> </a> </div></div><div id="ss_next_cav16" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-16" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/pi-3-kinase-p110-gamma-antibody_nbp2-15071#PublicationSection"> <div class="sprite_pubBook"></div> (3 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu, Mu, Rt</div><div class="tiny"><strong>Applications:</strong> ICC/IF, IHC, IHC-P, KO, WB</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="AF1230"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/erk2-antibody_af1230" style="overflow: hidden; width: 280px;" title="ERK2 Antibody [Unconjugated]">ERK2 Antibody<br> [Unconjugated]</a>    </div><div class="sub-header">AF1230</div> <div class="images_customers"><div class="one_image_l"></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-17" data-cycle-prev="#ss_prev_cav17" data-cycle-next="#ss_next_cav17" data-cycle-slides="div.big_thumb_cust_container" data-index=17 ><div class="big_thumb_cust_container"> <a href="/products/erk2-antibody_af1230" title="ERK2 Antibody [Unconjugated]" class="cust_viewed_17"> <img loading="lazy" src="/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png" alt="ERK2 Antibody [Unconjugated]" title="ERK2 Antibody [Unconjugated]" class=" big_thumb" /> </a> </div></div><div class="one_image"></div><div class="one_image_11"></div></div> <div class="ds_info"><div class="tiny"><strong>Species:</strong> Hu, Mu, Rt</div><div class="tiny"><strong>Applications:</strong> WB, IHC, KO</div> </div> </div> </div></div> </div><div id="cav_next"><div class="sprite_rightArrow"></div></div> <div class="clear"></div></div> <div class="ds_tab_content equalbottom" id="content_reviews-publications"> <a id="reviews-publications"></a><a id="PublicationSection"></a><h2 class="greyHead2">Publications for ERK2 Antibody (AF1230)(3)</h2><div class="ds_info"> <div>We have publications tested in 1 confirmed species: Human.<br /><br />We have publications tested in 1 application: Functional Assay.<br /><br /><div class="align-right"> <button class="greybutton btn_width" name="novusreview" onclick="window.location='/publications'"; >Submit a Publication</button><br /> </div> </div><div> <div class="filterParent"> <div class="publications_filterList" id="pubapp"> <div class="blueHead">Filter By Application</div><div class="filter_line"> <input type="checkbox" value="application" class="publication_filterCheckbox" data-filter="Functional Assay"> <div style="display:inline;">Functional Assay</div> (1)</div><div class="filter_clear" data-type="publication" data-filter="application">All Applications</div></div> <div class="publications_filterList" id="pubspec"> <div class="blueHead">Filter By Species</div><div class="filter_line"> <input type="checkbox" value="species" class="publication_filterCheckbox" data-filter="Human"> <div style="display:inline;">Human</div> (1)</div><div class="filter_clear" data-type="publication" data-filter="species">All Species</div></div> </div></div> <div class="clear"></div> <div id="publications_list">Showing Publications 1 - <span id="appcnt">3</span> of 3.<table class="wide lightPad tablesorter" id="publications_list_table"> <thead> <tr> <th>Publications using AF1230</th> <th>Applications</th> <th>Species</th> </tr> </thead> <tbody><tr class="lined revfil firstten" data-application="Functional Assay" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/31229924" target="_blank" rel="nofollow"> C Barale, C Frascaroli, F Cavalot, I Russo Hypercholesterolemia impairs the Glucagon-like peptide 1 action on platelets: Effects of a lipid-lowering treatment with simvastatin Thromb. Res., 2019-06-15;180(0):74-85. 2019-06-15 [PMID: 31229924] (Functional Assay, Human) </a></td> <td valign="top">Functional Assay</td> <td valign="top">Human</td> </tr><tr class="lined revfil firstten" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/25016365" target="_blank" rel="nofollow"> Paula Clancy, Simon A. Koblar, Jonathan Golledge Angiotensin receptor 1 blockade reduces secretion of inflammation associated cytokines from cultured human carotid atheroma and vascular cells in association with reduced extracellular signal regulated kinase expression and activation Atherosclerosis 9/1/2014 [PMID: 25016365] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil firstten" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/22174782" target="_blank" rel="nofollow"> Nandini V. L. Hayes, Lyne Jossé, C. Mark Smales, Martin J. Carden Modulation of Phosducin-Like Protein 3 (PhLP3) Levels Promotes Cytoskeletal Remodelling in a MAPK and RhoA-Dependent Manner PLoS ONE 12/9/2011 [PMID: 22174782] </a></td> <td valign="top"></td> <td valign="top"></td> </tr></tbody> </table> </div> </div> <a id="ReviewsSection"></a> <h2 class="greyHead2">Reviews for <span>ERK2 Antibody (AF1230) (0) </span></h2> <div class="ds_info"> There are no reviews for ERK2 Antibody (AF1230). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount. <br/> <ul> <li>Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen</li> <li>Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen</li> </ul> <div class="align-right"> <a href="/reviews/AF1230"><button class="greybutton btn_width" name="novusreview">Submit a Review</button></a><br /> </div> </div> <div class="clear"></div></div> <div class="ds_tab_content equalbottom" id="content_protocols-faqs"> <a id="protocols-faqs"></a><h2 class="greyHead2">Product General Protocols</h2> <a id="protocols"></a> <div class="ds_info">Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars. <ul><li><a href="/support/support-by-application/western-blot/protocol.html">Western Blot</a></li><li><a href="/support/support-by-application.html">View all Protocols, Troubleshooting, Illustrated assays and Webinars.</a></li> </ul></div><h2 class="greyHead2 ds_info">Video Protocols</h2> <a id="videos"></a> <div class="ds_info"><div class="movie-cell"> <span class="movie-title">WB Video Protocol</span><br/> <meta content ="https://www.youtube.com/watch?v=uFu8aie4QFI" /> <a href="https://www.youtube.com/embed/uFu8aie4QFI?rel=0&wmode=transparent" class="youtube"> <div class="reduce sprite_WBvideo"></div> </a> </div></div> <div class="clear"></div><h2 class="greyHead2">FAQs for ERK2 Antibody (AF1230) (0)</h2> <a id="faqs"></a> <div class="ds_info">There are no specific FAQs related to this product. Read our general <a href="/support/faqs.html">customer & technical service FAQs.</a> </div> <div class="clear"></div></div> <div class="ds_tab_content equalbottom" id="content_supportresearch"> <a id="supportresearch"></a><table cellpadding="0" cellspacing="0"><tr><td valign="top" class="width50"> <h2 class="greyHead2">Secondary Antibodies</h2> <a id="secondaryantibodies"></a> <div class="ds_info"> <ul class="information-list"><li><a href="/product-type/secondary-antibodies?species=Rabbit&facet_conjugate=HRP">Anti-Rabbit HRP Labeled Antibodies</a></li><li><a href="/product-type/secondary-antibodies?species=Rabbit&facet_conjugate=FITC">Anti-Rabbit FITC Labeled Antibodies</a></li><li><a href="/product-type/secondary-antibodies?species=Rabbit&facet_conjugate=Biotin">Anti-Rabbit Biotin Labeled Antibodies</a></li><li> </li> <li><a href="/product-type/secondary-antibodies#fq=species%3A%22Rabbit%22">View all of our anti-Rabbit Secondary Antibodies</a></li> </ul> </div></td><td width="5"> </td><td valign="top"> <h2 class="greyHead2" >Isotype Controls</h2> <a id="isotypecontrols"></a> <div class="ds_info"> <ul class="ds_noDotList"><li><a href="/product-type/isotype-controls#fq=species%3A%22Rabbit%22">Rabbit Isotype Controls</a></li><li> </li> <li><a href="/product-type/isotype-controls">View all of our Isotype Control Products</a></li> </div></td></tr></table><h2 class="greyHead2">Additional ERK2 Products</h2> <a id="addtlalphaprods"></a> <div class="ds_info"> <div> <span style="display:none;">Array AF1230</span> <ul class="ds_noDotList"><li> <a href="/primary-antibodies/erk2">ERK2 Antibodies</a></li><li> <a href="/lysates/erk2">ERK2 Lysates</a></li><li> <a href="/proteins/erk2">ERK2 Proteins</a></li><li> <a href="/elisas/erk2">ERK2 ELISA</a></li><li> <a href="/proteins-and-enzymes/erk2">ERK2 Proteins and Enzyme</a></li></ul> </div> </div><table cellpadding="0" cellspacing="0" class="width100"><tr></tr></table><table cellpadding="0" cellspacing="0" class="width100"><tr></tr></table><h2 class="greyHead2">Blogs on ERK2. </h2> <a id="blogsect"></a> <div class="ds_info"><a href="/antibody-news/tag/erk2">Check out the latest blog posts on ERK2</a>.</div> <div class="ds_info"> <table id="blogs_list_table"> <tbody> <tr class=" blogwrap "> <td> <strong><a href="/antibody-news/antibodies/the-effects-of-curcumin-on-ikb-alpha-and-the-nfkb-signaling-pathway">The effects of curcumin on IKB Alpha and the NFkB signaling pathway</a></strong><br/> <span>The IKK complex, or inhibitor of NFkB kinase, is composed of IKK alpha and IKK beta. These kinases are at the core of the NFkB signaling cascade. The NFkB family is made up of transcription factors that are kept inactive in the cytoplasm through...  <a href="/antibody-news/antibodies/the-effects-of-curcumin-on-ikb-alpha-and-the-nfkb-signaling-pathway">Read full blog post.</a></span><br /><br /> </td> </tr> <tr class=" blogwrap "> <td> <strong><a href="/antibody-news/antibodies/the-c-myc-antibody-a-major-tool-in-cancer-research">The c-Myc Antibody: A Major Tool in Cancer Research</a></strong><br/> <span>C-Myc is a widely expressed transcription factor, regulating cellular differentiation, proliferation, cell cycle progression and pro-apoptotic gene expression. The c-Myc antibody is widely used in cancer research, as a number of human tumors have been...  <a href="/antibody-news/antibodies/the-c-myc-antibody-a-major-tool-in-cancer-research">Read full blog post.</a></span><br /><br /> </td> </tr> <tr class=" blogwrap "> <td> <strong><a href="/antibody-news/antibodies/use-of-c-myc-antibodies-in-non-invasive-cancer-studies">Use Of c-Myc Antibodies In Non-Invasive Cancer Studies</a></strong><br/> <span>Novus Biologicals specializes in offering top quality antibody reagents to many of the transcription factor proteins that are widely expressed in cancer cells.C-Myc is one of several transcription factor proteins covered by our antibody catalog. Enc...  <a href="/antibody-news/antibodies/use-of-c-myc-antibodies-in-non-invasive-cancer-studies">Read full blog post.</a></span><br /><br /> </td> </tr></tbody> </table> </div> <div class="clear"></div></div> </div></div><div class="grid_6"><div class="ds_blue_border marTop26 equalbottom"><div class="datasheet-ads-container" ><a href="/products/mfluor-violet-fluorescent-conjugated-antibodies" target="_parent"><img loading="lazy" src="https://images.novusbio.com/design/mfluor-violet-conjugated-antibodies-banner-final-v5-349x119.jpg" alt="mFluor Violet Conjugated Antibodies" title="mFluor Violet Conjugated Antibodies" class="datasheet-adzone" loading="lazy"></a></div><a id="jump_recommended"></a> <h2 class="greyHead2">Customers Who Bought This Also Bought</h2> <div class="text_center"><div class="images2"><div class="cycle-slideshow" data-cycle-fx="scrollHorz" data-cycle-timeout="0" data-cycle-prev="#ss_prev_sidebar" data-cycle-next="#ss_next_sidebar" data-cycle-slides="div.big_thumb_container" id="ds_customer_who_bought" ><div class="big_thumb_container" data-catnums="AB-105-C"> <div class="img_container"> <a href="/products/igg-isotype-control_ab-105-c" title="HepG2 human hepatocellular carcinoma cell line was stained with Rabbit Anti-Human Klotho beta Monoclonal Antibody (Catalog # MAB58891, filled histogram) or Rabbit IgG Isotype Control Antibody (Catalog # AB-105-C, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). View our protocol for Staining Membrane-associated Proteins." class="cust_bought" > <img loading="lazy" src="https://images.novusbio.com/images2/IgG_AB105C_Control_20512.jpg" title="HepG2 human hepatocellular carcinoma cell line was stained with Rabbit Anti-Human Klotho beta Monoclonal Antibody (Catalog # MAB58891, filled histogram) or Rabbit IgG Isotype Control Antibody (Catalog # AB-105-C, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). View our protocol for Staining Membrane-associated Proteins." alt="HepG2 human hepatocellular carcinoma cell line was stained with Rabbit Anti-Human Klotho beta Monoclonal Antibody (Catalog # MAB58891, filled histogram) or Rabbit IgG Isotype Control Antibody (Catalog # AB-105-C, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" /> </a> </div> <div class="caption_container"><a href="/products/igg-isotype-control_ab-105-c">Rabbit IgG Isotype Control [Unconjugated]</a> <br />AB-105-C</div> <div style="text-align: right"><button class="greybutton" id="novusviewdatasheet" name="novusviewdatasheet" onclick="window.location='/products/igg-isotype-control_ab-105-c';" >View Datasheet</button> </div> </div></div></div></div><div class="clear"></div><a id="jump_distributor"></a> <h2 class="greyHead2">Contact Information</h2> <div class="ds_info"> <div id="distributors"></div> </div> <div id="et_reload_trigger" style="display:none;">reload</div><h2 class="greyHead2">Product PDFs</h2><div class="silverHeadShort"> <span class="file"><a href="//aero.bio-techne.com/sds/pdf/af1230" target="_blank" class="sprite_dsPDF"><span class="pdfSmaller"> </span></a></span> <span class="download-file-text" ><a href="//aero.bio-techne.com/sds/pdf/af1230" target="_blank" class="datasheet-download sdsFile">PDF SDS</a></span> </div><div class="silverHeadShort"> <span class="file"><a href="//resources.rndsystems.com/pdfs/datasheets/af1230.pdf" target="_blank" class="sprite_dsPDF"><span class="pdfSmaller"> </span></a></span> <span class="download-file-text" ><a href="//resources.rndsystems.com/pdfs/datasheets/af1230.pdf" target="_blank" class="datasheet-download">PDF Datasheet</a></span> </div><div class="silverHeadShort"> <span class="file"><a href="mailto:novus@novusbio.com?subject=Request%20CofA%20for%20AF1230" class="sprite_newDocument"><span class="downloadBlank"> </span></a></span> <span class="download-file-text"><a href="mailto:nb-technical@bio-techne.com?subject=Request%20CofA%20for%20AF1230&body=Specific%20catalog%20number(if multiple sizes):%0ALot%20Number:%0AAny%20additional%20products:">Request CofA</a></span> </div><h2 class="greyHead2">Calculators</h2> <div class="calc_wrapper"> <div class="dsSprite_calc"></div> <div class="fake_link" id="calc_reconstitution_link">Concentration Calculator</div> <div id="calc_reconstitution_wrapper" class="calc_function_wrapper"> <div class="calc_popup"> <div class="calc_close"> <div class="dsSprite_calc_close"></div> </div> <div class="calc_header">Concentration Calculator</div> <div id="reconstitution_calculator" class="calculator"> <div class="reconstitution_calculator_text" id="reconstitution_calculator_instructions"> <p>The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. 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ERK2 Antibody [Unconjugated] (AF1230): Novus Biologicals