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RNAscope HiPlex Pro for COMET | Bio-Techne

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id="system-breadcrumb" class="visually-hidden">Breadcrumb</h2> <ol> <li> <a href="/">Home</a> </li> <li> <a href="/reagents">Bio-Techne Reagents</a> </li> <li> <a href="/reagents/rnascope-ish-technology">RNAscope ISH Technology</a> </li> <li> <a href="/reagents/rnascope-ish-technology/rnascope">RNAscope</a> </li> <li> <a href="/reagents/rnascope-ish-technology/rnascope/fluorescent-assays">RNAscope Fluorescent Assays</a> </li> <li> RNAscope HiPlex Pro For COMET </li> </ol> </nav> </div> </div> </div> </div> <main> <section class="section"> <div> <div id="block-bio-techne-global-content" class="system_main_block"> <article> <div> <div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><a href="#prodov">Product Overview</a> | <a href="#prodfe">Product Features</a> | <a href="#tech overview">Technology Overview</a> | <a href="#Featured applications">Featured Applications</a> | <a href="#images">Image Gallery</a> | <a href="#resources">Resources</a> | <a href="#order">Ordering Information</a></p></div> </div> </div> <div> <div class="paragraph paragraph--type--header"> <div> <div><h2><a name="prodov"></a>Product Overview</h2></div> </div> </div> </div> <div> <div class="paragraph paragraph--type--_-2-text-to-media mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_lg_sngl_frmtd_text_global"> <div><p>RNAscope™ HiPlex Pro was developed by ACD and<a href="http://lunaphore.com/" target="_blank"> Lunaphore </a>to detect up to 12 RNA targets in a method that is optimized for RNA and protein co-detection based on seqIF™ (sequential immunofluorescence) on Lunaphore's hyperplex platform, <a href="https://lunaphore.com/products/comet/" target="_blank">COMET™</a>. Combining both assays RNAscope HiPlex Pro with seqIF on COMET provides provides end-users with a fully-automated, same-section assay integrating multiplex RNA and multiplex protein data without compromising panel flexibility or sensitivity. The combined workflow allows users to select any 12 RNAscope targets and up to 24 protein targets using off-the-shelf, non-conjugated primary antibodies. The assay is designed to be compatible with current procedures on COMET using a unique protease-free pretreatment to promote streamlined, spatial multiomic data acquisition with subcellular resolution.</p></div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/rnascope-hiplex-pro-comet.png" alt="RNAscope Hiplex on comet image " loading="lazy" > </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--header"> <div> <div><h2><a name="prodfe"></a>Product Features</h2></div> </div> </div> </div> <div> <div class="paragraph paragraph--type--card-paragraph paragraph--view-mode--default card-para-default"> <div class="row card_row"> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: #f5f5f5; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/icons/icon_multiomics_light_blue.png" alt="Multiomics Icon" loading="lazy" > </div> </div> </div> <h3 class="card_title_htag"> <div><h3>Same-Section Multiomics</h3></div> </h3> <div class="card_description flex-grow-1"> <div><ul> <li> <p>Single workflow for co-detection of RNA and protein targets on the same tissue section</p> </li> <li> <p>Generate spatially resolved multiomic data at subcellular resolution</p> </li> </ul></div> </div> <div class="card-bottom-link"></div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: #f5f5f5; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/icons/agile-process_light_blue.png" alt="Workflow Icon" loading="lazy" > </div> </div> </div> <h3 class="card_title_htag"> <div><h3>Fully-Automated Workflow</h3></div> </h3> <div class="card_description flex-grow-1"> <div><ul> <li>Automated from target probe hybridization and protein detection to imaging</li> <li>Stain up to 4 slides in every run</li> </ul></div> </div> <div class="card-bottom-link"></div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: #f5f5f5; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/icons/icon_flexible-1_light_blue.png" alt="Flexible Icon" loading="lazy" > </div> </div> </div> <h3 class="card_title_htag"> <div><h3>Flexible Assay Design</h3></div> </h3> <div class="card_description flex-grow-1"> <div><ul><li>Use our extensive RNAscope probe catalog or design <a href="/reagents/rnascope-ish-technology/probes/custom/request-form">custom probes</a> for RNAs</li><li>Design custom panels</li></ul></div> </div> <div class="card-bottom-link"></div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--full-page-text-with-media mb-4 paragraph--view-mode--default"> <div><h2>Technology Overview</h2></div> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/multiomics-workflow-comet-rnascope-banner.png" alt="Multiomics Workflow COMET RNAscope Banner" loading="lazy" > </div> <div><p><strong><a name="tech overview"></a>Fully-Automated, Protease-Free Workflow:&nbsp; </strong>Automated probe hybridization, signal amplification, labeling, imaging and cleaving follow the multiomic-optimized deparaffinization and <a href="/applications/imaging/immunohistochemistry/epitope-retrieval">antigen retrieval</a> steps. Each round allows the detection of 4 RNA targets for up to 3 rounds which can be followed by seqIF protocols.</p><p><a name="Featured applications"></a></p></div> </div> </div> <div> <div class="paragraph paragraph--type--card-paragraph paragraph--view-mode--default card-para-default"> <h2>Featured Applications</h2> <div class="row card_row"> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: #f5f5f5; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/icons/immune-checkpoint_light_blue.png" alt="Immune Checkpoint Icon " loading="lazy" > </div> </div> </div> <h3 class="card_title_htag"> <div><h3>Immunology</h3></div> </h3> <div class="card_description flex-grow-1"> <div><ul><li>Assess cytokine profiles in inflamed tissues and identify the cellular source of inflammatory molecules</li><li>Visualize gene signatures with cell-specific markers to assess activation states of immune cells</li></ul></div> </div> <div class="card-bottom-link"></div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/applications/spatial-biology/rnascope-cancer" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: #f5f5f5; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/icons/cancer_light_blue.png" alt="Cancer Icon" loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="/applications/spatial-biology/rnascope-cancer" > <h3 class="card_title_htag"> <div><h3>Immuno-oncology</h3></div> </h3> </a> <div class="card_description flex-grow-1"> <div><ul><li>Evaluate the tumor immune context for a better therapeutic outcome</li><li>Study tumor heterogeneity by visualizing unique morphology and expression profiles</li></ul></div> </div> <div class="card-bottom-link"> <div> <a href="/applications/spatial-biology/rnascope-cancer" > Learn More </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: #f5f5f5; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/icons/virus_light_blue.png" alt="Virus Icon" loading="lazy" > </div> </div> </div> <h3 class="card_title_htag"> <div><h3>Immunity and Infectious Disease</h3></div> </h3> <div class="card_description flex-grow-1"> <div><ul> <li>Localization—visualizing infected cell types and tissues enables elucidation of viral transmission, pathogen-host interactions, and localization of hidden reservoirs</li> <li>Differential detection—targeting the sense or anti-sense strand enables detection and differentiation of virus during latent or active stages</li> <li>Coinfection detection—detect multiple viruses or co-infection</li> </ul></div> </div> <div class="card-bottom-link"></div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><a name="images"></a></p></div> </div> </div> <div> <div class="paragraph paragraph--type--_-2-text-to-media mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_header_2_global"> <div><h2>Cytokine Detection with Multiomics on COMET</h2></div> </div> <div class="field_block:paragraph:2_2_text_to_media:field_lg_sngl_frmtd_text_global"> <div><h4>Sample Type: FFPE human breast cancer​</h4><h4>RNA: IL6 (green)​</h4><h4>Protein: CD3 (yellow), Vimentin (orange), aSMA (blue)</h4></div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/reagents/rnascope-fluorescent-detection-hiplex-v2.jpg" alt="Multiomics on COMET​: FFPE human breast cancer​ RNA: IL6 (green)​ Protein: CD3 (yellow), Vimentin (orange), aSMA (blue)" loading="lazy" > </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--card-paragraph paragraph--view-mode--default card-para-default"> <div class="row card_row"> <div class="col-md-6"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/search?search_type=products&amp;keywords=RNAscope%20probes" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: #f5f5f5; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/zz-probe-3d-render-690x430.png" alt="RNAscope Probes" loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="/search?search_type=products&amp;keywords=RNAscope%20probes" > <h3 class="card_title_htag"> <div>Find RNAscope Probes</div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>Find probes below or <a href="/reagents/rnascope-ish-technology/probes/custom/request-form">request a custom design</a> if you don’t see your target.</p></div> </div> <div class="card-bottom-link"> <div> <a href="/search?search_type=products&amp;keywords=RNAscope%20probes" > Start Exploring </a> </div> </div> </div> </div> </div> <div class="col-md-6"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="https://lunaphore.com/" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: #f5f5f5; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/bt-antibodies-hero.png" alt="Antibody Hero Cell" loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="https://lunaphore.com/" > <h3 class="card_title_htag"> <div>Search Antibodies</div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>Work with off-the-shelf, <a href="/search?keywords=antibodies&amp;category_attr=Primary%20Antibodies"><strong>non-conjugated primary antibodies</strong></a>&nbsp;or quickly start using SPYRE Antibody Panels and detection reagents from Lunaphore.​</p></div> </div> <div class="card-bottom-link"> <div> <a href="https://lunaphore.com/" > Learn More </a> </div> </div> </div> </div> </div> </div> </div> </div> <div> <section class="paragraph paragraph--type--full-width-banner-with-image mb-4 paragraph--view-mode--default paragraph-background-color--dark"> <div class="banner_background_image" data-bgimage="https://resources.bio-techne.com/bio-techne-assets/images/banners/v8_round-black-blue-gradient-banner-1110x350.png" style="background-repeat: no-repeat;"> <div> <div> <div> <div class="paragraph paragraph--type--_-2-text-to-media mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_lg_sngl_frmtd_text_global"> <div><h2>Get Started Today. Speak With A Specialist.</h2></div> </div> <div class="field_block:paragraph:2_2_text_to_media:field_call_to_action_global"> <a href="https://info.bio-techne.com/ACD-Contact-Us.html" class="btn btn-primary" type="link"> Contact Us </a> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/icons/feature_icon_discuss_dark_blue.png" alt="Discuss Icon" loading="lazy" > </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </section> </div> <div> <div class="paragraph paragraph--type--header"> <div> <div><h2><a name="resources"></a>Resources</h2></div> </div> </div> </div> <div> <div class="paragraph paragraph--type--card-paragraph paragraph--view-mode--default card-para-default"> <div class="row card_row"> <div class="col-md-6"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="https://resources.bio-techne.com/bio-techne-assets/docs/pdfs/flyers/automated-multiomics-on-comet-flyer.pdf" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: #f5f5f5; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/literature/multiomics-brochure-teaser.png" alt="Multiomics Brochure Thumbnail" loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="https://resources.bio-techne.com/bio-techne-assets/docs/pdfs/flyers/automated-multiomics-on-comet-flyer.pdf" > <h3 class="card_title_htag"></h3> </a> <div class="card_description flex-grow-1"> <div><h3>Automated Multiomics on COMET</h3></div> </div> <div class="card-bottom-link"> <div> <a href="https://resources.bio-techne.com/bio-techne-assets/docs/pdfs/flyers/automated-multiomics-on-comet-flyer.pdf" > Download the Flyer </a> </div> </div> </div> </div> </div> <div class="col-md-6"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/resources/literature/comet-streamlines-hyperplex-immunofluorescence-tech-note" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: #f5f5f5; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/literature/technote-comet-streamlines-thumb.png" alt="COMET streamlines hyperplex immunofluorescence at single-cell resolution Tech Note" loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="/resources/literature/comet-streamlines-hyperplex-immunofluorescence-tech-note" > <h3 class="card_title_htag"></h3> </a> <div class="card_description flex-grow-1"> <div><h3>COMET streamlines hyperplex immunofluorescence at single-cell resolution</h3></div> </div> <div class="card-bottom-link"> <div> <a href="/resources/literature/comet-streamlines-hyperplex-immunofluorescence-tech-note" > Download the Tech Note </a> </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--card-paragraph paragraph--view-mode--default card-para-default"> <div class="row card_row"> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/resources/literature/sitc-2023-integration-of-rna-ish-and-seqif-for-automated-multiomic-analysis-of-tme-poster" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: ; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/posters/lunaphore-sp-integration-of-rna.png" alt="SITC2023 poster thumbnails" loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" 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