Recombinant Mouse JAM-C Fc Chimera Protein, CF (1213-J3): Novus Biologicals

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5.1724696046-473375462.1712005244" style="margin: 0 10px;" target="_blank"><img alt="Novus Biologicals products are now on bio-techne.com" src="https://images.novusbio.com/design/ready-to-buy-novus-products-login.png" style="height:120px; width:940px" /></a></div> </div> </div> </div> <h1>Recombinant Mouse JAM-C Fc Chimera Protein, CF</h1> </div> <div class="clear"></div> <div> <div class="region region-content"> <div id="block-et-messages-et-messages-block" class="block block-et-messages"> <div class="content"> <span></span> </div> </div> <div id="block-system-main" class="block block-system"> <div class="content"> <div class="grid_16"><div class="ds_info float_left"><a href="#PublicationSection"><div class="sprite_pubBook_large"></div></a> <a href="#PublicationSection" class="bottom2"><span class="ctr">(5 Publications)</span></a></div><div class="ds_info float_left"> <a href="/common-name/jam-c"><div class="sprite_allRelated"></div></a> <a href="/common-name/jam-c" class="bottom4"><span class="ctr">See All Related</span></a> </div><div class="ds_info float_left"><a href="/reviews/1213-J3"><div class="dsSprite_submitreview"></div></a> <a href="/reviews/1213-J3" class="bottom4"><span class="ctr">Submit a Review</span></a></div></div></div><div class="modalWrapper" style="display:none;"> <h2>Species Glossary</h2> <hr class="customhr"/> <img src="//images.novusbio.com/design/bunny.gif" alt="Species Glossary Rabbit" style="float: right;"> <table class="modalTable"> <tr> <td>At</td> <td>=</td> <td>A. thaliana</td> <td>SyHa</td> <td>=</td> <td>Golden Syrian Hamster</td> </tr> <tr> <td>All</td> <td>=</td> <td>All Species</td> <td>Ha</td> <td>=</td> <td>Hamster</td> </tr> <tr> <td>An</td> <td>=</td> <td>Animal</td> <td>Hu</td> <td>=</td> <td>Human</td> </tr> <tr> <td>ArHa</td> <td>=</td> <td>Armenian Hamster</td> <td>I</td> <td>=</td> <td>Insect</td> </tr> <tr> <td>Av</td> <td>=</td> <td>Avian</td> <td>Ll</td> <td>=</td> <td>Llama</td> </tr> <tr> <td>Bb</td> <td>=</td> <td>Baboon</td> <td>Ma</td> <td>=</td> <td>Mammal</td> </tr> <tr> <td>Ba</td> <td>=</td> <td>Bacteria</td> <td>Mk</td> <td>=</td> <td>Monkey</td> </tr> <tr> <td>Bv</td> <td>=</td> <td>Bovine</td> <td>Mu</td> <td>=</td> <td>Mouse</td> </tr> <tr> <td>Ca</td> <td>=</td> <td>Canine</td> <td>Multi</td> <td>=</td> <td>Multi-species</td> </tr> <tr> <td>Ce</td> <td>=</td> <td>C. Elegans</td> <td>NA</td> <td>=</td> <td>Non-species specific</td> </tr> <tr> <td>Ch</td> <td>=</td> <td>Chicken</td> <td>Or</td> <td>=</td> <td>Orangutan</td> </tr> <tr> <td>ChHa</td> <td>=</td> <td>Chinese Hamster</td> <td>Pl</td> <td>=</td> <td>Plant</td> </tr> <tr> <td>Cm</td> <td>=</td> <td>Cynomolgus Monkey</td> <td>Pm</td> <td>=</td> <td>Primate</td> </tr> <tr> <td>Do</td> <td>=</td> <td>Donkey</td> <td>Po</td> <td>=</td> <td>Porcine</td> </tr> <tr> <td>Dr</td> <td>=</td> <td>Drosophilia</td> <td>Rb</td> <td>=</td> <td>Rabbit</td> </tr> <tr> <td>Ec</td> <td>=</td> <td>E. Coli</td> <td>Rt</td> <td>=</td> <td>Rat</td> </tr> <tr> <td>Eq</td> <td>=</td> <td>Equine</td> <td>RM</td> <td>=</td> <td>Rhesus Macaque</td> </tr> <tr> <td>Fe</td> <td>=</td> <td>Feline</td> <td>Sh</td> <td>=</td> <td>Sheep</td> </tr> <tr> <td>Ft</td> <td>=</td> <td>Ferret</td> <td>Vb</td> <td>=</td> <td>Vertebrate</td> </tr> <tr> <td>Fi</td> <td>=</td> <td>Fish</td> <td>Vi</td> <td>=</td> <td>Virus</td> </tr> <tr> <td>Fu</td> <td>=</td> <td>Fungi</td> <td>Xp</td> <td>=</td> <td>Xenopus</td> </tr> <tr> <td>Ge</td> <td>=</td> <td>Gerbil</td> <td>Ye</td> <td>=</td> <td>Yeast</td> </tr> <tr> <td>GP</td> <td>=</td> <td>Guinea Pig</td> <td>Ze</td> <td>=</td> <td>Zebrafish</td> </tr> <tr> <td>Gt</td> <td>=</td> <td>Goat</td> </tr> </table> <div style="text-align: center;margin-left:25px;margin-right:25px;"> <p>Note: Mouseover a species abbreviation on the product page to display the fullname.</p> </div> </div> </div><div class="clear"></div><div class="grid_10 pos_relative"><div class="ds_blue_border equaltop"><div class="imagesleft equaltop"><h2 class="blueHead">Images</h2> <div class="midBlue"> </div><div class="ds_info"> <div> <div class="images_center sprite_novus_large"></div> <div class="alt-caption padding_left5" >There are currently no images for JAM-C (1213-J3).<br /><br />Every product we sell is backed by <a href="/guarantee">Novus' 100% Guarantee</a>. If you have used this product, please <a href="/reviews/1213-J3">submit your images and reviews to earn reward points</a>.</div> </div></div></div><div class="productsright pos_relative equaltop"><h2 class="blueHead">Product Details</h2> <div class="midBlue">Summary</div><table class="ds_list"><tr class="lined"> <td><strong>Reactivity</strong></td> <td><span title='Mouse'>Mu</span><span class="myModal">Species Glossary</span></td> </tr><tr class="lined"> <td><strong>Applications</strong></td> <td><span title='Bioactivity'>Bioactivity</span></td> </tr><tr class="lined"> <td><strong>Format</strong></td> <td><div>Carrier-Free</div></td> </tr></table></div> </div></div><div class="grid_6"><div></div> <div class="ds_blue_border equaltop"> <h2 class="blueHead_nopadding">Order Details</h2><ul class="ds_details"><li class="add-to-cart" data-type="full" data-prodname="Recombinant Mouse JAM-C Fc Chimera Protein, CF" data-catnum="1213-J3" data-catnums="1213-J3-050" data-disabled=''><noscript><form autocomplete="off" action="/products/recombinant-mouse-jam-c-fc-chimera-protein-cf_1213-j3" method="post" id="et-add-multiple-to-cart-form" accept-charset="UTF-8"><div><table class="sticky-enabled"> <thead><tr><th>Size</th><th>Price</th> </tr></thead> <tbody> <tr class="odd"><td><div class="atc_size" title="catalog # 1213-J3-050">50 ug</div><div data-msg='' class="atc_catnum">1213-J3-050</div></td><td><div style="font-size:11px;"><a class="jump_distributor" href="#jump_distributor">Contact Your<br />Distributor</a></div></td> </tr> <tr class="even"><td colspan="3" class="stock_row">4-6 Business Days</td> </tr> </tbody> </table> <input type="hidden" name="form_build_id" value="form-2L1se68C4qrb8fzXGiM9SqzCC3MuKIMOCYQdexMDNIQ" /> <input type="hidden" name="form_id" value="et_add_multiple_to_cart_form" /> </div></form></noscript></li></ul><div id="shipping" class="hidden"></td></tr></div><div class="conj_form_buttons"></div><div id="shipping_statement"><span id="shipping_rate"></span></div><div class="shipping_obt_transfer"><span>Novus Biologicals is part of Bio-Techne</span><br><br><a href="https://www.bio-techne.com/p/proteins-enzymes/recombinant-mouse-jam-c-fc-chimera-protein-cf_1213-j3" rel="follow" class="btn btn-primary">Shop this product on bio-techne.com</a></div><br><div class="learn_more"> <a href="/guarantee"> <div class="sprite_noImage" title="Novus Biologicals, part of Bio-Techne, offers 100% Guarantee"></div> <div class="learn_more_text">Novus Biologicals, part of Bio-Techne, offers 100% Guarantee</div></a> </div><span id="conj_form" class="hidden"><tr><td colspan="4"></td></tr></span> </div> </div><div class="clear"></div><div class="grid_10"><div><div id="ds_tabs" style="display:none;"> <div class="ds_tab_active" id="dsTabs_datasheet" data-anchor_desc="datasheet">Datasheet</div> <div class="ds_tab" id="dsTabs_reviews-publications" data-anchor_desc="reviews-publications">Reviews & Publications</div> <div class="ds_tab" id="dsTabs_protocols-faqs" data-anchor_desc="protocols-faqs">Protocols & FAQs</div> <div class="ds_tab" id="dsTabs_supportresearch" data-anchor_desc="supportresearch">Support & Research</div> </div><div class="ds_tab_content equalbottom" id="content_datasheet" > <a id="datasheet"></a><h2 class=greyHead2>Recombinant Mouse JAM-C Fc Chimera Protein, CF Summary</h2> <div class="ds_info"> <a id="tab1details"></a> <table class="ds_list wide" style="table-layout:fixed;"> <tr class="lined"> <td><strong>Details of Functionality</strong></td> <td><div>Measured by its ability to inhibit the adhesion of J45.01 human acute lymphoblastic leukemia T lymphocytes on immobilized JAM-2 (VE-JAM)/Fc Chimera. The ED<SUB>50</SUB> for this effect is 0.1-0.5 µg/mL in the presence of 1 x 10<SUP>5</SUP> cells/well. Each well was coated with 100 µL of a 0.2 µg/mL solution of rhJAM-2 (VE-JAM)/Fc Chimera.</div></td> </tr><tr class="lined"> <td><strong>Source</strong></td> <td><div>Mouse myeloma cell line, NS0-derived mouse JAM-C protein<br /><table> <tbody> <tr> <td style="border: 1pt solid black; width: 192px;" class="TableText7" align="middle">Mouse JAM-C<br>(Val32 - Asn241)<br>Accession # Q9D8B7</td> <td style="border: 1pt solid black; width: 192px;" class="TableText7" align="middle">IEGRMD</td> <td style="border: 1pt solid black; width: 192px;" class="TableText7" align="middle">Human IgG<sub>1</sub><br>(Pro100 - Lys330)</td></tr> <tr> <td style="border-left: 0pt solid black; width: 192px;" class="TableText7" align="left">N-terminus</td> <td style="border-right: 0pt solid black; width: 192px;" class="TableText7" align="left"></td> <td style="border-right: 0pt solid black; width: 192px;" class="TableText7" align="right">C-terminus </td></tr> <tr></tr></tbody></table></div></td> </tr><tr class="lined"> <td><strong>Accession #</strong></td> <td><div><a href="http://www.ncbi.nlm.nih.gov/protein/Q9D8B7" target="_blank" >Q9D8B7</a></div></td> </tr><tr class="lined"> <td><strong>N-terminal Sequence</strong></td> <td><div>Val32</div></td> </tr><tr class="lined"> <td><strong>Structure / Form</strong></td> <td><div>Disulfide-linked homodimer</div></td> </tr><tr class="lined"> <td><strong> Protein/Peptide Type</strong></td> <td><div>Recombinant Proteins</div></td> </tr><tr class="lined"> <td><strong>Gene</strong></td> <td><div>Jam3</div></td> </tr><tr class="lined"> <td><strong>Purity</strong></td> <td><div>>95%, by SDS-PAGE under reducing conditions and visualized by silver stain</div></td> </tr><tr class="lined"> <td><strong>Endotoxin Note</strong></td> <td><div><0.10 EU per 1 μg of the protein by the LAL method.</div></td> </tr> </table> </div> <h2 class="greyHead2">Applications/Dilutions</h2> <div class="ds_info"> <a id="tab1appsdiluts"></a> <table class="ds_list wide"> <tr class="lined"> <td><strong>Dilutions</strong></td> <td> <ul class="ds_noDotList"><li>Bioactivity </li> </ul> </td> </tr><tr class="lined"> <td><strong>Theoretical MW</strong></td> <td><div>50 kDa (monomer).<br> Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.</div></td> </tr><tr class="lined"> <td><strong>SDS-PAGE</strong></td> <td><div>64 kDa, reducing conditions</div></td> </tr><tr class="lined"> <td><strong>Publications</strong></td> <td> <table> <tr> <td>Read <a href="#PublicationSection"> Publications</a> using<br/> 1213-J3 in the following applications:<br /><br /> <ul class="information-list"><li class="noIndent"><div class="information-subject"> <a href="#PublicationSection" class="filter_link" data-destination="publication" data-type="application" data-filter="Adhesion Assay">Adhesion Assay</a> </div><div class="information-data">1 publication</div></li><li class="noIndent"><div class="information-subject"> <a href="#PublicationSection" class="filter_link" data-destination="publication" data-type="application" data-filter="Bioassay">Bioassay</a> </div><div class="information-data">2 publications</div></li><li class="noIndent"><div class="information-subject"> <a href="#PublicationSection" class="filter_link" data-destination="publication" data-type="application" data-filter="Cell Culture">Cell Culture</a> </div><div class="information-data">1 publication</div></li><li class="noIndent"><div class="information-subject"> <a href="#PublicationSection" class="filter_link" data-destination="publication" data-type="application" data-filter="Flow Cytometry">Flow Cytometry</a> </div><div class="information-data">2 publications</div></li></ul></td> </tr> </table> </td> </tr> </table> </div> <h2 class="greyHead2">Packaging, Storage & Formulations</h2><div class="ds_info"><table class="ds_list"><tr class="lined"> <td><strong>Storage</strong></td> <td><div><b>Use a manual defrost freezer and avoid repeated freeze-thaw cycles.</b><UL><LI>12 months from date of receipt, -20 to -70 °C as supplied.</LI><LI>1 month, 2 to 8 °C under sterile conditions after reconstitution.</LI><LI>3 months, -20 to -70 °C under sterile conditions after reconstitution.</LI></UL></div></td> </tr><tr class="lined"> <td><strong>Buffer</strong></td> <td><div>Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl.</div></td> </tr><tr class="lined"> <td><strong>Purity</strong></td> <td><div>>95%, by SDS-PAGE under reducing conditions and visualized by silver stain</div></td> </tr><tr class="lined"> <td><strong>Reconstitution Instructions</strong></td> <td><div>Reconstitute at 100 μg/mL in sterile PBS.</div></td> </tr></table></div> <h2 class="greyHead2">Notes</h2><div class="ds_info"> <a id="tab1notes"></a> <div>This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.</div> </div><h2 class="greyHead2">Alternate Names for Recombinant Mouse JAM-C Fc Chimera Protein, CF</h2> <div class="ds_info"> <a id="tab1altnames"></a> <div class="information-list"> <ul><li>CD323</li><li>JAM-2</li><li>JAM3</li><li>JAMC</li><li>JAM-C</li><li>JAM-CFLJ14529</li><li>junctional adhesion molecule 3JAM-3</li><li>junctional adhesion molecule C</li></ul></div> </div> <h2 class="greyHead2">Background</h2> <div class="ds_info"> <a id="tab1background"></a> <div><P>The family of junctional adhesion molecules (JAM), comprised of at least three members, are type I transmembrane receptors belonging to the immunoglobulin (Ig) superfamily (1, 2). These proteins are localized in the tight junctions between endothelial cells or epithelial cells. Some family members are also found on blood leukocytes and platelets. Mouse JAM-C cDNA predicts a 310 amino acid (aa) residue  precursor protein with a putative 31 aa signal peptide, a 210 aa extracellular region containing two Ig domains, an 18 aa transmembrane domain and a 51 aa cytoplasmic domain containing a PDZ-binding motif and a PKC phosphorylation site (3). Mouse JAM-C shares 86% aa sequence identity with its human homologue. It also shares approximately 31% and 35% aa sequence homology with mouse JAM-A and JAM-B, respectively (2). Mouse JAM-C is highly expressed during embryogenesis. In adult tissues, mouse JAM-C is restricted to endothelial cells, lymph endothelial cells in the kidney, lymph node and Peyer’s patches where the protein can be localized to the high endothelial venules (3). Although human JAM-C is expressed on human platelets and a subset of leukocytes, mouse JAM-C expression was not detected on any mouse lymphocytes (4). In contrast to human JAM-C which show weak homotypic interactions, mouse JAM-C was reported to exhibit homotypic interactions (3). Mouse JAM-C has also been shown to have heterotypic interaction with JAM-B. It is likely that mouse JAM-C may play a role in lymphocyte transendothelial migration (4).</P> <P><EM>The nomenclature used for the JAM family proteins is confusing. VE-JAM has been referred to in the literature variously as JAM-B or JAM-C. Until further clarification, R&D Systems has adopted the nomenclature where both mouse and human VE-JAM are referred to as JAM-B.</EM></P><div class="backref"><OL> <LI>Chavakis, T. <EM>et al</EM>. (2003) Thromb. Haemost. <STRONG>89</STRONG>:13.</LI> <LI>Aurrand-Lions, M. <EM>et al</EM>. (2001) Blood <STRONG>98</STRONG>:3699.</LI> <LI>Aurrand-Lions, M. <EM>et al</EM>. (2001) J. Biol. Chem. <STRONG>276</STRONG>:2733. </LI> <LI>Johnson-Leger, C. <EM>et al</EM>. (2002) Blood <STRONG>100</STRONG>:25793.</LI></OL></div></div> </div><h2 class="greyHead2">Customers Who Viewed This Item Also Viewed...</h2> <a id="custalsoviewed"></a><div id="cav_prev"><div class="sprite_leftArrow"></div></div> <div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout=0 data-cycle-prev="#cav_prev" data-cycle-next="#cav_next" data-cycle-slides="div.ds_cav_group" id="ds_customer_also_viewed"><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="H00050848-M01"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/jam-a-antibody-2e3-1c8_h00050848-m01" style="overflow: hidden; width: 280px;" title="JAM-A Antibody (2E3-1C8)">JAM-A Antibody<br> (2E3-1C8)</a>    </div><div class="sub-header">H00050848-M01</div> <div class="images_customers"><div id="ss_prev_cav0" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-0" data-cycle-prev="#ss_prev_cav0" data-cycle-next="#ss_next_cav0" data-cycle-slides="div.big_thumb_cust_container" data-index=0 ><div class="big_thumb_cust_container"> <a href="/products/jam-a-antibody-2e3-1c8_h00050848-m01" title="Western Blot: JAM-A Antibody (2E3-1C8) [H00050848-M01] - Analysis of F11R expression in transfected 293T cell line by F11R monoclonal antibody (M01), clone 2E3-1C8. 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Not internally tested by Novus Biologicals." class="cust_viewed_0" > <img loading="lazy" src="https://images.novusbio.com/images/h00050848-m01_mouse-monoclonal-jam-a-antibody-2e3-1c8-310202415395914.jpg" alt="Immunohistochemistry-Paraffin JAM-A Antibody (2E3-1C8)" class="big_thumb" /> </a> </div></div><div id="ss_next_cav0" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-0" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/jam-a-antibody-2e3-1c8_h00050848-m01#PublicationSection"> <div class="sprite_pubBook"></div> (11 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu</div><div class="tiny"><strong>Applications:</strong> ELISA, ICC/IF, IHC, IHC-P, PLA, S-ELISA, WB</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="AF1074"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/jam-b-ve-jam-antibody_af1074" style="overflow: hidden; width: 280px;" title="JAM-B/VE-JAM Antibody [Unconjugated]">JAM-B/VE-JAM<br> Antibody [Unconjugate</a>    </div><div class="sub-header">AF1074</div> <div class="images_customers"><div id="ss_prev_cav1" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-1" data-cycle-prev="#ss_prev_cav1" data-cycle-next="#ss_next_cav1" data-cycle-slides="div.big_thumb_cust_container" data-index=1 ><div class="big_thumb_cust_container"> <a href="/products/jam-b-ve-jam-antibody_af1074" title="Western blot shows lysates of human placenta tissue and human testis tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse JAM-B/VE-JAM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1074) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for JAM-B/VE-JAM at approximately 43-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="cust_viewed_1" > <img loading="lazy" src="https://images.novusbio.com/images2/JAMB_AF1074_Western_Blot_21290.jpg" alt="Western Blot JAM-B/VE-JAM Antibody [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/jam-b-ve-jam-antibody_af1074" title="Western blot shows lysates of bEnd.3 mouse endothelioma cell line and SVEC4-10 mouse vascular endothelial cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human/Mouse JAM-B/VE-JAM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1074) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for JAM-B/VE-JAM at approximately 40-48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="cust_viewed_1" > <img loading="lazy" src="https://images.novusbio.com/images2/JAMB_AF1074_Western_Blot_21369.jpg" alt="Western Blot JAM-B/VE-JAM Antibody [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/jam-b-ve-jam-antibody_af1074" title=" Simple Western lane view shows lysates of human placenta tissue and human testis tissue, loaded at 0.2&amp;nbsp;mg/mL. A specific band was detected for JAM&amp;#8209;B/VE&amp;#8209;JAM at approximately 58&amp;#8209;61&amp;nbsp;kDa (as indicated) using 20&amp;nbsp;µg/mL of Goat Anti-Human JAM&amp;#8209;B/VE&amp;#8209;JAM Antigen Affinity-purified Polyclonal Antibody (Catalog&amp;nbsp;# AF1074) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog&amp;nbsp;# HAF109). This experiment was conducted under reducing conditions and using the 12&amp;#8209;230&amp;nbsp;kDa separation system. " class="cust_viewed_1" > <img loading="lazy" src="https://images.novusbio.com/images2/JAMB_AF1074_Simple_Western_21685.jpg" alt="Simple Western JAM-B/VE-JAM Antibody [Unconjugated]" class="big_thumb" /> </a> </div></div><div id="ss_next_cav1" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-1" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/jam-b-ve-jam-antibody_af1074#ReviewsSection"> <div class="sprite_4Stars"></div> (1 Review</a>) </div> <div class="tiny"> <a href="/products/jam-b-ve-jam-antibody_af1074#PublicationSection"> <div class="sprite_pubBook"></div> (2 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu, Mu</div><div class="tiny"><strong>Applications:</strong> IHC, Neut, Simple Western, WB</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="AF1730"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/integrin-beta-2-cd18-antibody_af1730" style="overflow: hidden; width: 280px;" title="Integrin beta 2/CD18 Antibody [Unconjugated]">Integrin beta<br> 2/CD18 Antibody [Unco ...</a>    </div><div class="sub-header">AF1730</div> <div class="images_customers"><div id="ss_prev_cav2" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-2" data-cycle-prev="#ss_prev_cav2" data-cycle-next="#ss_next_cav2" data-cycle-slides="div.big_thumb_cust_container" data-index=2 ><div class="big_thumb_cust_container"> <a href="/products/integrin-beta-2-cd18-antibody_af1730" title="Integrin beta 2/CD18 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using 10 &amp;micro;g/mL Goat Anti-Human Integrin beta 2/CD18 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1730) for 3 hours at room temperature. Cells were stained with the NorthernLights&amp;trade; 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="cust_viewed_2" > <img loading="lazy" src="https://images.novusbio.com/images2/Integrin_beta_2_AF1730_Immunocytochemistry_6474.jpg" alt="Immunocytochemistry Integrin beta 2/CD18 Antibody [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/integrin-beta-2-cd18-antibody_af1730" title="Integrin&amp;#160; beta 2/CD18 was detected in immersion fixed THP&amp;#x2011;1 human acute monocytic leukemia cells (Positive) &amp; absent in RT&amp;#x2011;4 human urinary bladder transitional cell papilloma (Negative) using Goat Anti-Human Integrin&amp;#160; beta 2/CD18 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1730) at 5&amp;#160;&amp;micro;g/mL for 3 hours at room temperature. Cells were stained using the NorthernLights&amp;trade; 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog&amp;#160;# NL001) and counterstained with DAPI (blue). Specific staining was localized to Cell surface. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="cust_viewed_2" > <img loading="lazy" src="https://images.novusbio.com/images/antibody/af1730_human-integrin-beta-2-cd18-affinity-purified-polyclonal-ab-immunocytochemistry-1292023225343.jpg" alt="Immunocytochemistry Integrin beta 2/CD18 Antibody [Unconjugated]" class="big_thumb" /> </a> </div></div><div id="ss_next_cav2" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-2" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/integrin-beta-2-cd18-antibody_af1730#PublicationSection"> <div class="sprite_pubBook"></div> (16 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu</div><div class="tiny"><strong>Applications:</strong> AdBlk, CyTOF-ready, Flow, ICC</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NB110-89474"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/cd11b-antibody_nb110-89474" style="overflow: hidden; width: 280px;" title="CD11b Antibody - BSA Free">CD11b Antibody -<br> BSA Free</a>    </div><div class="sub-header">NB110-89474</div> <div class="images_customers"><div id="ss_prev_cav3" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-3" data-cycle-prev="#ss_prev_cav3" data-cycle-next="#ss_next_cav3" data-cycle-slides="div.big_thumb_cust_container" data-index=3 ><div class="big_thumb_cust_container"> <a href="/products/cd11b-antibody_nb110-89474" title="Immunocytochemistry/Immunofluorescence: CD11b Antibody - BSA Free [NB110-89474] - Raw264.7 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD11b Antibody conjugated to Alexa Fluor 488 (NB110-89474AF488) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved." class="cust_viewed_3" > <img loading="lazy" src="https://images.novusbio.com/images/CD11b-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB110-89474-img0028.jpg" alt="Immunocytochemistry/ Immunofluorescence CD11b Antibody - BSA Free" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/cd11b-antibody_nb110-89474" title="Immunocytochemistry/Immunofluorescence: CD11b Antibody - BSA Free [NB110-89474] - Raw264.7 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD11b Antibody conjugated to Alexa Fluor 647 (NB110-89474AF647) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved." class="cust_viewed_3" > <img loading="lazy" src="https://images.novusbio.com/images/CD11b-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB110-89474-img0029.jpg" alt="Immunocytochemistry/ Immunofluorescence CD11b Antibody - BSA Free" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/cd11b-antibody_nb110-89474" title="Immunocytochemistry/Immunofluorescence: CD11b Antibody - BSA Free [NB110-89474] - Raw264.7 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD11b Antibody conjugated to DyLight 650 (NB110-8947C) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved." class="cust_viewed_3" > <img loading="lazy" src="https://images.novusbio.com/images/CD11b-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB110-89474-img0030.jpg" alt="Immunocytochemistry/ Immunofluorescence CD11b Antibody - BSA Free" class="big_thumb" /> </a> </div></div><div id="ss_next_cav3" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-3" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/cd11b-antibody_nb110-89474#ReviewsSection"> <div class="sprite_5Stars"></div> (12 Reviews</a>) </div> <div class="tiny"> <a href="/products/cd11b-antibody_nb110-89474#PublicationSection"> <div class="sprite_pubBook"></div> (114 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Bv, Ce, ChHa, Hu, Pm, Mu, Rt, RM</div><div class="tiny"><strong>Applications:</strong> Dual ISH-IHC, Flow-CS, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, ISH, Simple Western, Single-Cell Western, WB</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="AF3449"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/amica-jaml-antibody_af3449" style="overflow: hidden; width: 280px;" title="AMICA/JAML Antibody">AMICA/JAML Antibody</a>    </div><div class="sub-header">AF3449</div> <div class="images_customers"><div class="one_image_l"></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-4" data-cycle-prev="#ss_prev_cav4" data-cycle-next="#ss_next_cav4" data-cycle-slides="div.big_thumb_cust_container" data-index=4 ><div class="big_thumb_cust_container"> <a href="/products/amica-jaml-antibody_af3449" title="AMICA/JAML Antibody" class="cust_viewed_4"> <img loading="lazy" src="/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png" alt="AMICA/JAML Antibody" title="AMICA/JAML Antibody" class=" big_thumb" /> </a> </div></div><div class="one_image"></div><div class="one_image_11"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/amica-jaml-antibody_af3449#PublicationSection"> <div class="sprite_pubBook"></div> (1 Publication</a>) </div><div class="tiny"><strong>Species:</strong> Hu</div><div class="tiny"><strong>Applications:</strong> CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, WB</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NBP1-85047"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/tight-junction-protein-1-antibody_nbp1-85047" style="overflow: hidden; width: 280px;" title="Tight Junction Protein 1 Antibody">Tight Junction<br> Protein 1 Antibody</a>    </div><div class="sub-header">NBP1-85047</div> <div class="images_customers"><div id="ss_prev_cav5" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-5" data-cycle-prev="#ss_prev_cav5" data-cycle-next="#ss_next_cav5" data-cycle-slides="div.big_thumb_cust_container" data-index=5 ><div class="big_thumb_cust_container"> <a href="/products/tight-junction-protein-1-antibody_nbp1-85047" title="Immunocytochemistry/Immunofluorescence: Tight Junction Protein 1 Antibody [NBP1-85047] - Staining of Pig trabecular meshwork cells. ICC/IF image submitted by a verified customer review." class="cust_viewed_5" > <img loading="lazy" src="https://images.novusbio.com/images/Tight-Junction-Protein-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85047-img0013.jpg" alt="Immunocytochemistry/ Immunofluorescence Tight Junction Protein 1 Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/tight-junction-protein-1-antibody_nbp1-85047" title="Immunocytochemistry/Immunofluorescence: Tight Junction Protein 1 Antibody [NBP1-85047] - Staining of human cell line U-2 OS shows localization to cytosol &amp; cell junctions. Antibody staining is shown in green." class="cust_viewed_5" > <img loading="lazy" src="https://images.novusbio.com/images/Tight-Junction-Protein-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85047-img0008.jpg" alt="Immunocytochemistry/ Immunofluorescence Tight Junction Protein 1 Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/tight-junction-protein-1-antibody_nbp1-85047" title="Immunohistochemistry-Paraffin: Tight Junction Protein 1 Antibody [NBP1-85047] - Staining of human kidney shows strong membranous positivity in cells in glomeruli." class="cust_viewed_5" > <img loading="lazy" src="https://images.novusbio.com/images/Tight-Junction-Protein-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85047-img0014.jpg" alt="Immunohistochemistry-Paraffin Tight Junction Protein 1 Antibody" class="big_thumb" /> </a> </div></div><div id="ss_next_cav5" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-5" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/tight-junction-protein-1-antibody_nbp1-85047#ReviewsSection"> <div class="sprite_5Stars"></div> (5 Reviews</a>) </div> <div class="tiny"> <a href="/products/tight-junction-protein-1-antibody_nbp1-85047#PublicationSection"> <div class="sprite_pubBook"></div> (20 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu, Mu, Po, Rt</div><div class="tiny"><strong>Applications:</strong> ICC/IF, IHC, IHC-P, Simple Western, WB</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="H00050848-M01"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/jam-a-antibody-2e3-1c8_h00050848-m01" style="overflow: hidden; width: 280px;" title="JAM-A Antibody (2E3-1C8)">JAM-A Antibody<br> (2E3-1C8)</a>    </div><div class="sub-header">H00050848-M01</div> <div class="images_customers"><div id="ss_prev_cav6" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-6" data-cycle-prev="#ss_prev_cav6" data-cycle-next="#ss_next_cav6" data-cycle-slides="div.big_thumb_cust_container" data-index=6 ><div class="big_thumb_cust_container"> <a href="/products/jam-a-antibody-2e3-1c8_h00050848-m01" title="Western Blot: JAM-A Antibody (2E3-1C8) [H00050848-M01] - Analysis of F11R expression in transfected 293T cell line by F11R monoclonal antibody (M01), clone 2E3-1C8. Lane 1: F11R transfected lysate (Predicted MW: 32.6 KDa). Lane 2: Non-transfected lysate." class="cust_viewed_6" > <img loading="lazy" src="https://images.novusbio.com/images/JAM-A-Antibody-2E3-1C8-Western-Blot-H00050848-M01-img0014.jpg" alt="Western Blot JAM-A Antibody (2E3-1C8)" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/jam-a-antibody-2e3-1c8_h00050848-m01" title="Immunohistochemistry: JAM-A Antibody (2E3-1C8) [H00050848-M01] - Coronal sections of human meninges including the retrobulbar, midorbital, and intracanalicular part of the optic nerve stained for cell-cell interaction markers of tight junctions (JAM-A, Occludin, and Claudin 5). A representative staining of one patient is shown. * indicates arachnoid layer. Image collected and cropped by CiteAb from the following publication (https://journal.frontiersin.org/article/10.3389/fneur.2017.00308/full) licensed under a CC-BY license." class="cust_viewed_6" > <img loading="lazy" src="https://images.novusbio.com/images/JAM-A-Antibody-2E3-1C8-Immunohistochemistry-H00050848-M01-img0016.jpg" alt="Immunohistochemistry JAM-A Antibody (2E3-1C8)" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/jam-a-antibody-2e3-1c8_h00050848-m01" title="Immunohistochemistry-Paraffin: JAM-A Antibody (2E3-1C8) [H00050848-M01] - Sagittal sections of human meninges including the retrobulbar, midorbital, &amp; intracanalicular part of the optic nerve stained for cell–cell interaction markers of tight junctions (JAM1, Occludin, &amp; Claudin 5), gap junctions (Connexin 26 &amp; 43), &amp; desmosomes (Desmoplakin). A representative staining of one patient is shown. * indicates arachnoid layer. Image collected &amp; cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28706505), licensed under a CC-BY license. Not internally tested by Novus Biologicals." class="cust_viewed_6" > <img loading="lazy" src="https://images.novusbio.com/images/h00050848-m01_mouse-monoclonal-jam-a-antibody-2e3-1c8-310202415395914.jpg" alt="Immunohistochemistry-Paraffin JAM-A Antibody (2E3-1C8)" class="big_thumb" /> </a> </div></div><div id="ss_next_cav6" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-6" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/jam-a-antibody-2e3-1c8_h00050848-m01#PublicationSection"> <div class="sprite_pubBook"></div> (11 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu</div><div class="tiny"><strong>Applications:</strong> ELISA, ICC/IF, IHC, IHC-P, PLA, S-ELISA, WB</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="AF629"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/lymphotoxin-beta-r-tnfrsf3-antibody_af629" style="overflow: hidden; width: 280px;" title="Lymphotoxin beta R/TNFRSF3 Antibody">Lymphotoxin beta<br> R/TNFRSF3 Antibody</a>    </div><div class="sub-header">AF629</div> <div class="images_customers"><div class="one_image_l"></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-7" data-cycle-prev="#ss_prev_cav7" data-cycle-next="#ss_next_cav7" data-cycle-slides="div.big_thumb_cust_container" data-index=7 ><div class="big_thumb_cust_container"> <a href="/products/lymphotoxin-beta-r-tnfrsf3-antibody_af629" title="Lymphotoxin beta R/TNFRSF3 Antibody" class="cust_viewed_7"> <img loading="lazy" src="/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png" alt="Lymphotoxin beta R/TNFRSF3 Antibody" title="Lymphotoxin beta R/TNFRSF3 Antibody" class=" big_thumb" /> </a> </div></div><div class="one_image"></div><div class="one_image_11"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/lymphotoxin-beta-r-tnfrsf3-antibody_af629#PublicationSection"> <div class="sprite_pubBook"></div> (10 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu</div><div class="tiny"><strong>Applications:</strong> CyTOF-ready, Flow, InhibCellGro, WB</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NBP2-02259"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/claudin-5-antibody-oti1g4_nbp2-02259" style="overflow: hidden; width: 280px;" title="Claudin-5 Antibody (OTI1G4)">Claudin-5 Antibody<br> (OTI1G4)</a>    </div><div class="sub-header">NBP2-02259</div> <div class="images_customers"><div id="ss_prev_cav8" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-8" data-cycle-prev="#ss_prev_cav8" data-cycle-next="#ss_next_cav8" data-cycle-slides="div.big_thumb_cust_container" data-index=8 ><div class="big_thumb_cust_container"> <a href="/products/claudin-5-antibody-oti1g4_nbp2-02259" title="Immunocytochemistry/Immunofluorescence: Claudin-5 Antibody (1G4) [NBP2-02259] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Claudin 5." class="cust_viewed_8" > <img loading="lazy" src="https://images.novusbio.com/images/Claudin-5-Antibody-1G4-Immunocytochemistry-Immunofluorescence-NBP2-02259-img0002.jpg" alt="Immunocytochemistry/ Immunofluorescence Claudin-5 Antibody (OTI1G4)" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/claudin-5-antibody-oti1g4_nbp2-02259" title="Immunocytochemistry/Immunofluorescence: Claudin-5 Antibody (OTI1G4) [NBP2-02259] - Claudin-5 labeled pig SC cells visualized by alexa 594. ICC/IF image submitted by a verified customer review." class="cust_viewed_8" > <img loading="lazy" src="https://images.novusbio.com/images/Claudin-5-Antibody-1G4-Immunocytochemistry-NBP2-02259-img0003.jpg" alt="Immunocytochemistry/ Immunofluorescence Claudin-5 Antibody (OTI1G4)" class="big_thumb" /> </a> </div></div><div id="ss_next_cav8" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-8" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/claudin-5-antibody-oti1g4_nbp2-02259#ReviewsSection"> <div class="sprite_5Stars"></div> (1 Review</a>) </div><div class="tiny"><strong>Species:</strong> Hu, Mu, Po, Rt</div><div class="tiny"><strong>Applications:</strong> ICC/IF</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NBP1-87402"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/occludin-antibody_nbp1-87402" style="overflow: hidden; width: 280px;" title="Occludin Antibody">Occludin Antibody</a>    </div><div class="sub-header">NBP1-87402</div> <div class="images_customers"><div id="ss_prev_cav9" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-9" data-cycle-prev="#ss_prev_cav9" data-cycle-next="#ss_next_cav9" data-cycle-slides="div.big_thumb_cust_container" data-index=9 ><div class="big_thumb_cust_container"> <a href="/products/occludin-antibody_nbp1-87402" title="Western Blot: Occludin Antibody [NBP1-87402] - Lane 1: untreated vascular endothelial cells Lane 2: vascular endothelial cells + control siRNA. Primary antibody was diluted 1:1000. WB image submitted by a verified customer review." class="cust_viewed_9" > <img loading="lazy" src="https://images.novusbio.com/images/Occludin-Antibody-Western-Blot-NBP1-87402-img0009.jpg" alt="Western Blot Occludin Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/occludin-antibody_nbp1-87402" title="Immunohistochemistry-Paraffin: Occludin Antibody [NBP1-87402] - Staining of human breast cancer shows moderate membranous positivity in tumor cells." class="cust_viewed_9" > <img loading="lazy" src="https://images.novusbio.com/images/Occludin-Antibody-Immunohistochemistry-Paraffin-NBP1-87402-img0016.jpg" alt="Immunohistochemistry-Paraffin Occludin Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/occludin-antibody_nbp1-87402" title="Immunohistochemistry-Paraffin: Occludin Antibody [NBP1-87402] - Staining of human prostate cancer shows moderate membranous positivity in tumor cells." class="cust_viewed_9" > <img loading="lazy" src="https://images.novusbio.com/images/Occludin-Antibody-Immunohistochemistry-Paraffin-NBP1-87402-img0017.jpg" alt="Immunohistochemistry-Paraffin Occludin Antibody" class="big_thumb" /> </a> </div></div><div id="ss_next_cav9" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-9" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/occludin-antibody_nbp1-87402#ReviewsSection"> <div class="sprite_4Stars"></div> (4 Reviews</a>) </div> <div class="tiny"> <a href="/products/occludin-antibody_nbp1-87402#PublicationSection"> <div class="sprite_pubBook"></div> (23 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu, Mu, Rt</div><div class="tiny"><strong>Applications:</strong> ELISA, IHC, IHC-P, KD, WB</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="AF3628"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/cd31-pecam-1-antibody_af3628" style="overflow: hidden; width: 280px;" title="CD31/PECAM-1 Antibody [Unconjugated]">CD31/PECAM-1<br> Antibody [Unconjugate</a>    </div><div class="sub-header">AF3628</div> <div class="images_customers"><div id="ss_prev_cav10" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-10" data-cycle-prev="#ss_prev_cav10" data-cycle-next="#ss_next_cav10" data-cycle-slides="div.big_thumb_cust_container" data-index=10 ><div class="big_thumb_cust_container"> <a href="/products/cd31-pecam-1-antibody_af3628" title="CD31/PECAM-1 was detected in immersion fixed frozen sections of mouse embryo (E13.5) using Goat Anti-Human/Mouse/Rat CD31/PECAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights&amp;#8482; 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; NL001) and counterstained with DAPI (blue). Specific staining was localized to developing endothelium. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." class="cust_viewed_10" > <img loading="lazy" src="https://images.novusbio.com/images2/CD31_AF3628_Immunohistochemistry_10287.jpg" alt="Immunohistochemistry CD31/PECAM-1 Antibody [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/cd31-pecam-1-antibody_af3628" title="Rat splenocytes were stained with Goat Anti-Human/Mouse/Rat CD31/PECAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628, filled histogram) or isotype control antibody (AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (F0108)." class="cust_viewed_10" > <img loading="lazy" src="https://images.novusbio.com/images2/CD31_AF3628_Flow_Cytometry_16017.jpg" alt="Flow Cytometry CD31/PECAM-1 Antibody [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/cd31-pecam-1-antibody_af3628" title="CD31/PECAM-1 was detected in immersion fixed frozen sections of mouse embryo (14 d.p.c.) using Goat Anti-Human/Mouse/Rat CD31/PECAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte&amp;#8482; HRP Polymer Antibody (VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to developing guts. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="cust_viewed_10" > <img loading="lazy" src="https://images.novusbio.com/images2/CD31_AF3628_Immunohistochemistry_21486.jpg" alt="Immunohistochemistry CD31/PECAM-1 Antibody [Unconjugated]" class="big_thumb" /> </a> </div></div><div id="ss_next_cav10" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-10" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/cd31-pecam-1-antibody_af3628#ReviewsSection"> <div class="sprite_5Stars"></div> (6 Reviews</a>) </div> <div class="tiny"> <a href="/products/cd31-pecam-1-antibody_af3628#PublicationSection"> <div class="sprite_pubBook"></div> (494 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu, Mu, Rt</div><div class="tiny"><strong>Applications:</strong> CyTOF-ready, Flow, ICC, IHC, Simple Western, WB</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NBP1-88861"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/pard3-par3-antibody_nbp1-88861" style="overflow: hidden; width: 280px;" title="PARD3/Par3 Antibody">PARD3/Par3 Antibody</a>    </div><div class="sub-header">NBP1-88861</div> <div class="images_customers"><div id="ss_prev_cav11" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-11" data-cycle-prev="#ss_prev_cav11" data-cycle-next="#ss_next_cav11" data-cycle-slides="div.big_thumb_cust_container" data-index=11 ><div class="big_thumb_cust_container"> <a href="/products/pard3-par3-antibody_nbp1-88861" title="Immunohistochemistry-Paraffin: PARD3/Par3 Antibody [NBP1-88861] - Staining of human kidney shows weak to moderate membranous positivity in cells in tubules." class="cust_viewed_11" > <img loading="lazy" src="https://images.novusbio.com/images/PARD3-Par3-Antibody-Immunohistochemistry-Paraffin-NBP1-88861-img0019.jpg" alt="Immunohistochemistry-Paraffin PARD3/Par3 Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/pard3-par3-antibody_nbp1-88861" title="Immunohistochemistry-Paraffin: PARD3/Par3 Antibody [NBP1-88861] - Staining of human cerebellum shows moderate positivity in neuropil." class="cust_viewed_11" > <img loading="lazy" src="https://images.novusbio.com/images/PARD3-Par3-Antibody-Immunohistochemistry-Paraffin-NBP1-88861-img0020.jpg" alt="Immunohistochemistry-Paraffin PARD3/Par3 Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/pard3-par3-antibody_nbp1-88861" title="Immunohistochemistry-Paraffin: PARD3/Par3 Antibody [NBP1-88861] - Staining of human fallopian tube shows weak to moderate positivity in luminal membrane in glandular cells." class="cust_viewed_11" > <img loading="lazy" src="https://images.novusbio.com/images/PARD3-Par3-Antibody-Immunohistochemistry-Paraffin-NBP1-88861-img0021.jpg" alt="Immunohistochemistry-Paraffin PARD3/Par3 Antibody" class="big_thumb" /> </a> </div></div><div id="ss_next_cav11" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-11" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/pard3-par3-antibody_nbp1-88861#ReviewsSection"> <div class="sprite_4Stars"></div> (2 Reviews</a>) </div> <div class="tiny"> <a href="/products/pard3-par3-antibody_nbp1-88861#PublicationSection"> <div class="sprite_pubBook"></div> (10 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu, Mu</div><div class="tiny"><strong>Applications:</strong> ICC/IF, IHC, IHC-P, IP, Simple Western, WB</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NB100-524"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/nod2-antibody-2d9_nb100-524" style="overflow: hidden; width: 280px;" title="NOD2 Antibody (2D9) - BSA Free">NOD2 Antibody<br> (2D9) - BSA Free</a>    </div><div class="sub-header">NB100-524</div> <div class="images_customers"><div id="ss_prev_cav12" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-12" data-cycle-prev="#ss_prev_cav12" data-cycle-next="#ss_next_cav12" data-cycle-slides="div.big_thumb_cust_container" data-index=12 ><div class="big_thumb_cust_container"> <a href="/products/nod2-antibody-2d9_nb100-524" title="Flow Cytometry: NOD2 Antibody (2D9) [NB100-524] - An intracellular stain was performed on Jurkat cells with NOD2 (2D9) antibody NB100-524APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes." class="cust_viewed_12" > <img loading="lazy" src="https://images.novusbio.com/images/NOD2-Antibody-2D9-Flow-Cytometry-NB100-524-img0014.jpg" alt="Flow Cytometry NOD2 Antibody (2D9) - BSA Free" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/nod2-antibody-2d9_nb100-524" title="Flow Cytometry: NOD2 Antibody (2D9) [NB100-524] - An intracellular stain was performed on Jurkat cells with NOD2 (2D9) antibody NB100-524PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes.Both antibodies were conjugated to phycoerythrin." class="cust_viewed_12" > <img loading="lazy" src="https://images.novusbio.com/images/NOD2-Antibody-2D9-Flow-Cytometry-NB100-524-img0015.jpg" alt="Flow Cytometry NOD2 Antibody (2D9) - BSA Free" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/nod2-antibody-2d9_nb100-524" title="Immunohistochemistry-Frozen: NOD2 Antibody (2D9) [NB100-524] - Overlay of NOD2-DyLight 488 (green) with phase contrast of murine colon. Image from verified customer review." class="cust_viewed_12" > <img loading="lazy" src="https://images.novusbio.com/images/NOD2-Antibody-2D9-Immunohistochemistry-Frozen-NB100-524-img0016.jpg" alt="Immunohistochemistry-Frozen NOD2 Antibody (2D9) - BSA Free" class="big_thumb" /> </a> </div></div><div id="ss_next_cav12" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-12" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/nod2-antibody-2d9_nb100-524#ReviewsSection"> <div class="sprite_5Stars"></div> (1 Review</a>) </div> <div class="tiny"> <a href="/products/nod2-antibody-2d9_nb100-524#PublicationSection"> <div class="sprite_pubBook"></div> (26 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu, Mu</div><div class="tiny"><strong>Applications:</strong> Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NBP2-79843"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/hla-dq-dr-dp-antibody-hla-pan-2967r_nbp2-79843" style="overflow: hidden; width: 280px;" title="HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free">HLA DQ/DR/DP<br> Antibody (HLA-Pan/296 ...</a>    </div><div class="sub-header">NBP2-79843</div> <div class="images_customers"><div id="ss_prev_cav13" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-13" data-cycle-prev="#ss_prev_cav13" data-cycle-next="#ss_next_cav13" data-cycle-slides="div.big_thumb_cust_container" data-index=13 ><div class="big_thumb_cust_container"> <a href="/products/hla-dq-dr-dp-antibody-hla-pan-2967r_nbp2-79843" title="Protein Array: HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free [NBP2-79843] - Analysis of Protein Array containing more than 19,000 full-length human proteins using HLA DQ/DR/DP Antibody (HLA-Pan/2967R). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5." class="cust_viewed_13" > <img loading="lazy" src="https://images.novusbio.com/images/HLA-DQ-DR-DP-Antibody-HLA-Pan-2967R-Azide-and-BSA-Free-Protein-Array-NBP2-79843-img0001.jpg" alt="Protein Array HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/hla-dq-dr-dp-antibody-hla-pan-2967r_nbp2-79843" title="Immunocytochemistry/Immunofluorescence: HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free [NBP2-79843] - Immunofluorescence staining of Raji cells using HLA DQ/DR/DP Antibody (HLA-Pan/2967R) followed by goat anti-rabbit IgG-CF488 (Green). Nuclei stained with RedDot (Red)." class="cust_viewed_13" > <img loading="lazy" src="https://images.novusbio.com/images/HLA-DQ-DR-DP-Antibody-HLA-Pan-2967R-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-79843-img0002.jpg" alt="Immunocytochemistry/ Immunofluorescence HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/hla-dq-dr-dp-antibody-hla-pan-2967r_nbp2-79843" title="Western Blot: HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free [NBP2-79843] - Western Blot Analysis of Ramos cell lysate using HLA DQ/DR/DP Antibody (HLA-Pan/2967R)." class="cust_viewed_13" > <img loading="lazy" src="https://images.novusbio.com/images/HLA-DQ-DR-DP-Antibody-HLA-Pan-2967R-Azide-and-BSA-Free-Western-Blot-NBP2-79843-img0003.jpg" alt="Western Blot HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free" class="big_thumb" /> </a> </div></div><div id="ss_next_cav13" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-13" class="cycle-circle-pager"></div></div> <div class="ds_info"><div class="tiny"><strong>Species:</strong> Hu</div><div class="tiny"><strong>Applications:</strong> CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, WB</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="7268-CT"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/recombinant-human-ctla-4-fc-chimera-cho-expressed-cf_7268-ct" style="overflow: hidden; width: 280px;" title="CTLA-4 [Unconjugated]">CTLA-4<br> [Unconjugated]</a>    </div><div class="sub-header">7268-CT</div> <div class="images_customers"><div class="one_image_l"></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-14" data-cycle-prev="#ss_prev_cav14" data-cycle-next="#ss_next_cav14" data-cycle-slides="div.big_thumb_cust_container" data-index=14 ><div class="big_thumb_cust_container"> <a href="/products/recombinant-human-ctla-4-fc-chimera-cho-expressed-cf_7268-ct" title="Recombinant Human CTLA-4 Fc Chimera (Catalog # 7268-CT) inhibits IL-2 secretion by stimulated Jurkat human acute Tcell leukemia cells. The&amp;nbsp;ED50 for this effect is 0.03-0.15 μg/mL whenstimulated with 1 μg/mL Recombinant Human B7‑1/CD80 Fc Chimera (Catalog&amp;nbsp;# 140-B1). " class="cust_viewed_14" > <img loading="lazy" src="https://images.novusbio.com/images2/CTLA4_7268CT_2293.jpg" alt="Bioactivity CTLA-4 [Unconjugated]" class="big_thumb" /> </a> </div></div><div class="one_image"></div><div class="one_image_11"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/recombinant-human-ctla-4-fc-chimera-cho-expressed-cf_7268-ct#PublicationSection"> <div class="sprite_pubBook"></div> (3 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu</div><div class="tiny"><strong>Applications:</strong> BA</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="AF2654"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/cxadr-antibody_af2654" style="overflow: hidden; width: 280px;" title="CXADR Antibody [Unconjugated]">CXADR Antibody<br> [Unconjugated]</a>    </div><div class="sub-header">AF2654</div> <div class="images_customers"><div id="ss_prev_cav15" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-15" data-cycle-prev="#ss_prev_cav15" data-cycle-next="#ss_next_cav15" data-cycle-slides="div.big_thumb_cust_container" data-index=15 ><div class="big_thumb_cust_container"> <a href="/products/cxadr-antibody_af2654" title="Western blot shows lysates of mouse liver tissue and mouse embryo tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse CXADR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2654) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for CXADR at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="cust_viewed_15" > <img loading="lazy" src="https://images.novusbio.com/images2/CXADR_AF2654_Western_Blot_17043.jpg" alt="Western Blot CXADR Antibody [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/cxadr-antibody_af2654" title=" Simple Western lane view shows lysates of mouse embryo tissue, loaded at 0.2&amp;nbsp;mg/mL. Specific bands were detected for CXADR at approximately 60 and 53&amp;nbsp;kDa (as indicated) using 12.5&amp;nbsp;µg/mL of Goat Anti-Mouse CXADR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2654) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog&amp;nbsp;# HAF109). This experiment was conducted under reducing&amp;nbsp;conditions and using the12-230 kDa separation system.&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;" class="cust_viewed_15" > <img loading="lazy" src="https://images.novusbio.com/images2/CXADR_AF2654_Immunohistochemistry_17641.jpg" alt="Simple Western CXADR Antibody [Unconjugated]" class="big_thumb" /> </a> </div></div><div id="ss_next_cav15" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-15" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/cxadr-antibody_af2654#PublicationSection"> <div class="sprite_pubBook"></div> (6 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Mu</div><div class="tiny"><strong>Applications:</strong> IHC, Simple Western, WB</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="137-PS"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/recombinant-human-p-selectin-cd62p-fc-chimera-protein-cf_137-ps" style="overflow: hidden; width: 280px;" title="P-Selectin/CD62P">P-Selectin/CD62P</a>    </div><div class="sub-header">137-PS</div> <div class="images_customers"><div class="one_image_l"></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-16" data-cycle-prev="#ss_prev_cav16" data-cycle-next="#ss_next_cav16" data-cycle-slides="div.big_thumb_cust_container" data-index=16 ><div class="big_thumb_cust_container"> <a href="/products/recombinant-human-p-selectin-cd62p-fc-chimera-protein-cf_137-ps" title="P-Selectin/CD62P" class="cust_viewed_16"> <img loading="lazy" src="/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png" alt="P-Selectin/CD62P" title="P-Selectin/CD62P" class=" big_thumb" /> </a> </div></div><div class="one_image"></div><div class="one_image_11"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/recombinant-human-p-selectin-cd62p-fc-chimera-protein-cf_137-ps#PublicationSection"> <div class="sprite_pubBook"></div> (46 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu</div><div class="tiny"><strong>Applications:</strong> BA</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="AF796"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/icam-1-cd54-antibody_af796" style="overflow: hidden; width: 280px;" title="ICAM-1/CD54 Antibody [Unconjugated]">ICAM-1/CD54<br> Antibody [Unconjugate</a>    </div><div class="sub-header">AF796</div> <div class="images_customers"><div id="ss_prev_cav17" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-17" data-cycle-prev="#ss_prev_cav17" data-cycle-next="#ss_next_cav17" data-cycle-slides="div.big_thumb_cust_container" data-index=17 ><div class="big_thumb_cust_container"> <a href="/products/icam-1-cd54-antibody_af796" title="ICAM-1/CD54 was detected in perfusion fixed frozen sections of mouse testis using Goat Anti-Mouse ICAM-1/CD54 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF796) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell &amp; Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane in Leydig cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="cust_viewed_17" > <img loading="lazy" src="https://images.novusbio.com/images2/ICAM1_AF796_Immunohistochemistry_17354.jpg" alt="Immunohistochemistry ICAM-1/CD54 Antibody [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/icam-1-cd54-antibody_af796" title="Increased stress kinase signaling and JNK pathway-dependent cytokine and chemokine production by primary keratinocytes lacking BRAF and RAF1.(A) Reduced ERK phosphorylation and increased JNK/p38 activation in primary delta / delta ep2 keratinocytes stimulated with EGF and/or TNF alpha and IL1 beta for 15 min. (B) Increased cytokine and chemokine production in primary delta / delta ep2 keratinocytes treated with EGF, TNF alpha and IL1 beta for 24 hr. Cytokine and chemokine production was determined by multiplex analysis, except for TSLP which was quantified by ELISA. Data represent mean ± SEM of 3–5 biological replicates. (C–D) Cells were pretreated with D-JNKI1 inhibitors prior to stimulation with EGF, TNF alpha and IL1 beta for 15 min (C) or 24 hr (D). Data represent the mean ± SEM of technical replicates (n = 3). (E–F) Effect of shRNA-mediated Mlk3 silencing on ERK and JNK phosphorylation and ICAM1 expression (E; stimulation with EGF, TNF alpha and IL1 beta for 15 min) and on the expression of Ccl2 and Tslp mRNA (F; stimulation with EGF, TNF alpha and IL1 beta for 24 hr) by F/F2 and delta / delta ep2 keratinocytes. shRen, shRNA targeting Renilla, used as a control; sh1 and sh2, targeting Mlk3, binding sites nucleotide 2266–2285 and 2383–2402, respectively. The shRNAs were encoded by lentiviral vectors coexpressing GFP. GFP immunoblots are shown to confirm similar levels of infection in all samples. Data represent mean ± SEM of 4 biological replicates. Each keratinocyte culture represents a pool of three mice. Immunoblots are representative of three independent experiments. p1 = 0.041, p2 = 0.040, p3 = 1.89E-4, p4 = 0.018, p5 = 0.046, p6 = 0.020, p7 = 0.008, p8 = 0.016, p9 = 0.001, p10 = 0.018, p11 = 3.23E-4, p12 = 1.47E-4, p13 = 0.007, p14 = 0.03, p15 = 0.035, p16 = 0.023 and p17 = 0.046.DOI:https://dx.doi.org/10.7554/eLife.14012.018Compound knockdown (KD2) of BRAF and RAF1 induce the expression of inflammation markers by HaCat cells in a MLK3/JNK-dependent manner.(A) Reduced ERK and increased JNK/p38 activation in BRAF and RAF1 knockdown (KD2) HaCat cells stimulated with EGF, TNF alpha and IL1 beta for 15 min. (B) D-JNKI1 reduces ICAM1 and CCL2 (n = 4) expression in KD2 cells treated with TNF alpha . (C) MEKi induces ICAM1 and CCL2 (n = 3) expression in RAF1KD cells treated with TNF alpha . In (B–C), ICAM1 expression was measured after a 3 hr, CCL2 expression after a 24 hr treatment with TNF alpha . (D) Effect of MLK3 silencing on ERK and JNK phosphorylation in WT and KD2 cells stimulated as in (A). MLK3 was silenced using a pool of oligonucleotides targeting the following regions: 686–704; 1489–1507; 2122–2138; and 2348–2366. MLK3 KD cells stimulated as in (B–C) show a decrease in JNK activation, ICAM1 and CCL2 (n = 7) expression. Immunoblots are representative of three independent experiments. qPCR data represent mean ± SEM of three independent experiments run in duplicates (p1 = 4.62E-4, p2 = 0.013, p3 = 0.050, p4 = 8.60E-8, p5 = 0.050, p6 = 0.001, p7 = 0.001 and p8 = 0.012).DOI:https://dx.doi.org/10.7554/eLife.14012.019 Image collected and cropped by CiteAb from the following publication (https://elifesciences.org/articles/14012), licensed under a CC-BY license. Not internally tested by R&amp;D Systems." class="cust_viewed_17" > <img loading="lazy" src="https://images.novusbio.com/images/af796_mouse-icam-1-cd54-affinity-purified-polyclonal-ab-4120241048112.jpg" alt="Western Blot ICAM-1/CD54 Antibody [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/icam-1-cd54-antibody_af796" title="Increased stress kinase signaling and JNK pathway-dependent cytokine and chemokine production by primary keratinocytes lacking BRAF and RAF1.(A) Reduced ERK phosphorylation and increased JNK/p38 activation in primary delta / delta ep2 keratinocytes stimulated with EGF and/or TNF alpha and IL1 beta for 15 min. (B) Increased cytokine and chemokine production in primary delta / delta ep2 keratinocytes treated with EGF, TNF alpha and IL1 beta for 24 hr. Cytokine and chemokine production was determined by multiplex analysis, except for TSLP which was quantified by ELISA. Data represent mean ± SEM of 3–5 biological replicates. (C–D) Cells were pretreated with D-JNKI1 inhibitors prior to stimulation with EGF, TNF alpha and IL1 beta for 15 min (C) or 24 hr (D). Data represent the mean ± SEM of technical replicates (n = 3). (E–F) Effect of shRNA-mediated Mlk3 silencing on ERK and JNK phosphorylation and ICAM1 expression (E; stimulation with EGF, TNF alpha and IL1 beta for 15 min) and on the expression of Ccl2 and Tslp mRNA (F; stimulation with EGF, TNF alpha and IL1 beta for 24 hr) by F/F2 and delta / delta ep2 keratinocytes. shRen, shRNA targeting Renilla, used as a control; sh1 and sh2, targeting Mlk3, binding sites nucleotide 2266–2285 and 2383–2402, respectively. The shRNAs were encoded by lentiviral vectors coexpressing GFP. GFP immunoblots are shown to confirm similar levels of infection in all samples. Data represent mean ± SEM of 4 biological replicates. Each keratinocyte culture represents a pool of three mice. Immunoblots are representative of three independent experiments. p1 = 0.041, p2 = 0.040, p3 = 1.89E-4, p4 = 0.018, p5 = 0.046, p6 = 0.020, p7 = 0.008, p8 = 0.016, p9 = 0.001, p10 = 0.018, p11 = 3.23E-4, p12 = 1.47E-4, p13 = 0.007, p14 = 0.03, p15 = 0.035, p16 = 0.023 and p17 = 0.046.DOI:https://dx.doi.org/10.7554/eLife.14012.018Compound knockdown (KD2) of BRAF and RAF1 induce the expression of inflammation markers by HaCat cells in a MLK3/JNK-dependent manner.(A) Reduced ERK and increased JNK/p38 activation in BRAF and RAF1 knockdown (KD2) HaCat cells stimulated with EGF, TNF alpha and IL1 beta for 15 min. (B) D-JNKI1 reduces ICAM1 and CCL2 (n = 4) expression in KD2 cells treated with TNF alpha . (C) MEKi induces ICAM1 and CCL2 (n = 3) expression in RAF1KD cells treated with TNF alpha . In (B–C), ICAM1 expression was measured after a 3 hr, CCL2 expression after a 24 hr treatment with TNF alpha . (D) Effect of MLK3 silencing on ERK and JNK phosphorylation in WT and KD2 cells stimulated as in (A). MLK3 was silenced using a pool of oligonucleotides targeting the following regions: 686–704; 1489–1507; 2122–2138; and 2348–2366. MLK3 KD cells stimulated as in (B–C) show a decrease in JNK activation, ICAM1 and CCL2 (n = 7) expression. Immunoblots are representative of three independent experiments. qPCR data represent mean ± SEM of three independent experiments run in duplicates (p1 = 4.62E-4, p2 = 0.013, p3 = 0.050, p4 = 8.60E-8, p5 = 0.050, p6 = 0.001, p7 = 0.001 and p8 = 0.012).DOI:https://dx.doi.org/10.7554/eLife.14012.019 Image collected and cropped by CiteAb from the following publication (https://elifesciences.org/articles/14012), licensed under a CC-BY license. Not internally tested by R&amp;D Systems." class="cust_viewed_17" > <img loading="lazy" src="https://images.novusbio.com/images/af796_mouse-icam-1-cd54-affinity-purified-polyclonal-ab-41202410481112.jpg" alt="Western Blot ICAM-1/CD54 Antibody [Unconjugated]" class="big_thumb" /> </a> </div></div><div id="ss_next_cav17" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-17" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/icam-1-cd54-antibody_af796#ReviewsSection"> <div class="sprite_5Stars"></div> (6 Reviews</a>) </div> <div class="tiny"> <a href="/products/icam-1-cd54-antibody_af796#PublicationSection"> <div class="sprite_pubBook"></div> (88 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Mu</div><div class="tiny"><strong>Applications:</strong> AdBlk, IHC, WB</div> </div> </div> </div></div> </div><div id="cav_next"><div class="sprite_rightArrow"></div></div> <div class="clear"></div></div> <div class="ds_tab_content equalbottom" id="content_reviews-publications"> <a id="reviews-publications"></a><a id="PublicationSection"></a><h2 class="greyHead2">Publications for JAM-C (1213-J3)(5)</h2><div class="ds_info"> <div>We have publications tested in 1 confirmed species: Mouse.<br /><br />We have publications tested in 4 applications: Adhesion Assay, Bioassay, Cell Culture, Flow Cytometry.<br /><br /><div class="align-right"> <button class="greybutton btn_width" name="novusreview" onclick="window.location='/publications'"; >Submit a Publication</button><br /> </div> </div><div> <div class="filterParent"> <div class="publications_filterList" id="pubapp"> <div class="blueHead">Filter By Application</div><div class="filter_line"> <input type="checkbox" value="application" class="publication_filterCheckbox" data-filter="Adhesion Assay"> <div style="display:inline;">Adhesion Assay</div> (1)</div><div class="filter_line"> <input type="checkbox" value="application" class="publication_filterCheckbox" data-filter="Bioassay"> <div style="display:inline;">Bioassay</div> (2)</div><div class="filter_line"> <input type="checkbox" value="application" class="publication_filterCheckbox" data-filter="Cell Culture"> <div style="display:inline;">Cell Culture</div> (1)</div><div class="filter_line"> <input type="checkbox" value="application" class="publication_filterCheckbox" data-filter="Flow Cytometry"> <div style="display:inline;">Flow Cytometry</div> (2)</div><div class="filter_clear" data-type="publication" data-filter="application">All Applications</div></div> <div class="publications_filterList" id="pubspec"> <div class="blueHead">Filter By Species</div><div class="filter_line"> <input type="checkbox" value="species" class="publication_filterCheckbox" data-filter="Mouse"> <div style="display:inline;">Mouse</div> (5)</div><div class="filter_clear" data-type="publication" data-filter="species">All Species</div></div> </div></div> <div class="clear"></div> <div id="publications_list">Showing Publications 1 - <span id="appcnt">5</span> of 5.<table class="wide lightPad tablesorter" id="publications_list_table"> <thead> <tr> <th>Publications using 1213-J3</th> <th>Applications</th> <th>Species</th> </tr> </thead> <tbody><tr class="lined revfil firstten" data-application="Cell Culture" data-species="Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/32054130" target="_blank" rel="nofollow"> M Czabanka, LL Petrilli, S Elvers-Hor, K Bieback, B Albert Imh, P Vajkoczy, M Vinci Junctional Adhesion Molecule-C Mediates the Recruitment of Embryonic-Endothelial Progenitor Cells to the Perivascular Niche during Tumor Angiogenesis Int J Mol Sci, 2020-02-11;21(4):. 2020-02-11 [PMID: 32054130] (Cell Culture, Mouse) </a></td> <td valign="top">Cell Culture</td> <td valign="top">Mouse</td> </tr><tr class="lined revfil firstten" data-application="Bioassay" data-species="Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/31167130" target="_blank" rel="nofollow"> V Radulovic, M van der Ga, S Koide, V Sigurdsson, S Lang, S Kaneko, K Miharada Junctional Adhesion Molecule 2 Represents a Subset of Hematopoietic Stem Cells with Enhanced Potential for T Lymphopoiesis Cell Rep, 2019-06-04;27(10):2826-2836.e5. 2019-06-04 [PMID: 31167130] (Bioassay, Mouse) </a></td> <td valign="top">Bioassay</td> <td valign="top">Mouse</td> </tr><tr class="lined revfil firstten" data-application="Flow Cytometry" data-species="Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/27111582" target="_blank" rel="nofollow"> E Hintermann, M Bayer, J Ehser, M Aurrand-Li, JM Pfeilschif, BA Imhof, U Christen Murine junctional adhesion molecules JAM-B and JAM-C mediate endothelial and stellate cell interactions during hepatic fibrosis Cell Adh Migr, 2016-04-25;10(4):419-33. 2016-04-25 [PMID: 27111582] (Flow Cytometry, Mouse) </a></td> <td valign="top">Flow Cytometry</td> <td valign="top">Mouse</td> </tr><tr class="lined revfil firstten" data-application="Bioassay" data-species="Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/22511936" target="_blank" rel="nofollow"> Su RW, Jia B, Ni H Junctional adhesion molecule 2 mediates the interaction between hatched blastocyst and luminal epithelium: induction by progesterone and LIF. PLoS ONE, 2012-04-12;7(4):e34325. 2012-04-12 [PMID: 22511936] (Bioassay, Mouse) </a></td> <td valign="top">Bioassay</td> <td valign="top">Mouse</td> </tr><tr class="lined revfil firstten" data-application="Adhesion Assay, Flow Cytometry" data-species="Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/21868569" target="_blank" rel="nofollow"> Arcangeli ML, Frontera V, Bardin F, Obrados E, Adams S, Chabannon C, Schiff C, Mancini SJ, Adams RH, Aurrand-Lions M JAM-B regulates maintenance of hematopoietic stem cells in the bone marrow. Blood, 2011-08-25;118(17):4609-19. 2011-08-25 [PMID: 21868569] (Adhesion Assay, Flow Cytometry, Mouse) </a></td> <td valign="top">Adhesion Assay, Flow Cytometry</td> <td valign="top">Mouse</td> </tr></tbody> </table> </div> </div> <a id="ReviewsSection"></a> <h2 class="greyHead2">Reviews for <span>JAM-C (1213-J3) (0) </span></h2> <div class="ds_info"> There are no reviews for JAM-C (1213-J3). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount. <br/> <ul> <li>Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen</li> <li>Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen</li> </ul> <div class="align-right"> <a href="/reviews/1213-J3"><button class="greybutton btn_width" name="novusreview">Submit a Review</button></a><br /> </div> </div> <div class="clear"></div></div> <div class="ds_tab_content equalbottom" id="content_protocols-faqs"> <a id="protocols-faqs"></a><h2 class="greyHead2">FAQs for JAM-C (1213-J3) (0)</h2> <a id="faqs"></a> <div class="ds_info">There are no specific FAQs related to this product. Read our general <a href="/support/faqs.html">customer & technical service FAQs.</a> </div> <div class="clear"></div></div> <div class="ds_tab_content equalbottom" id="content_supportresearch"> <a id="supportresearch"></a><table cellpadding="0" cellspacing="0"><tr></td></tr></table><h2 class="greyHead2">Additional JAM-C Products</h2> <a id="addtlalphaprods"></a> <div class="ds_info"> <div> <span style="display:none;">Array 1213-J3</span> <ul class="ds_noDotList"><li> <a href="/primary-antibodies/jam-c">JAM-C Antibodies</a></li><li> <a href="/elisa-kits/jam-c">JAM-C ELISA Kits</a></li><li> <a href="/lysates/jam-c">JAM-C Lysate</a></li><li> <a href="/proteins/jam-c">JAM-C Proteins</a></li><li> <a href="/proteins-and-enzymes/jam-c">JAM-C Proteins and Enzymes</a></li></ul> </div> </div><table cellpadding="0" cellspacing="0" class="width100"><tr></tr></table><table cellpadding="0" cellspacing="0" class="width100"><tr></tr></table><h2 class="greyHead2">Blogs on JAM-C</h2> <a id="blogsect"></a> <div>There are no specific blogs for JAM-C, but you can <a href="/antibody-news/">read our latest blog posts.</div> <div class="clear"></div></div> </div></div><div class="grid_6"><div class="ds_blue_border marTop26 equalbottom"><div class="datasheet-ads-container" ><a href="https://www.bio-techne.com/resources/blogs/citation-widget-find-products-used-in-literature" target="_blank"><img loading="lazy" src="https://images.novusbio.com/design/citation-app-banner-349x119-v3.jpg" alt="Read our latest blog and use the new citation tool on bio-techne.com" title="new citation tool" class="datasheet-adzone" loading="lazy"></a></div><a id="jump_recommended"></a> <h2 class="greyHead2">Customers Who Bought This Also Bought</h2> <div class="text_center"><div class="images2"><div id="ss_prev_sidebar" class="cycle-prev ds_rp_prev"><div class="pager_left_on"></div></div><div id="ss_next_sidebar" class="cycle-next ds_rp_next"><div class="pager_right_on"></div></div><div class="cycle-slideshow" data-cycle-fx="scrollHorz" data-cycle-timeout="0" data-cycle-prev="#ss_prev_sidebar" data-cycle-next="#ss_next_sidebar" data-cycle-slides="div.big_thumb_container" id="ds_customer_who_bought" ><div class="big_thumb_container" data-catnums="AF3628"> <div class="img_container"> <a href="/products/cd31-pecam-1-antibody_af3628" title="CD31/PECAM-1 was detected in immersion fixed frozen sections of mouse embryo (E13.5) using Goat Anti-Human/Mouse/Rat CD31/PECAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights&#8482; 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; NL001) and counterstained with DAPI (blue). Specific staining was localized to developing endothelium. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." class="cust_bought" > <img loading="lazy" src="https://images.novusbio.com/images2/CD31_AF3628_Immunohistochemistry_10287.jpg" title="CD31/PECAM-1 was detected in immersion fixed frozen sections of mouse embryo (E13.5) using Goat Anti-Human/Mouse/Rat CD31/PECAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights&#8482; 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; NL001) and counterstained with DAPI (blue). Specific staining was localized to developing endothelium. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." alt="CD31/PECAM-1 was detected in immersion fixed frozen sections of mouse embryo (E13.5) using Goat Anti-Human/Mouse/Rat CD31/PECAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights&#8482; 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; NL001) and counterstained with DAPI (blue). Specific staining was localized to developing endothelium. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." class="big_thumb" /> </a> </div> <div class="caption_container"><a href="/products/cd31-pecam-1-antibody_af3628">CD31/PECAM-1 Antibody [Unconjugated]</a> <br />AF3628</div> <div style="text-align: right"><button class="greybutton" id="novusviewdatasheet" name="novusviewdatasheet" onclick="window.location='/products/cd31-pecam-1-antibody_af3628';" >View Datasheet</button> </div> </div><div class="big_thumb_container" data-catnums="AF1213"> <div class="img_container"> <a href="/products/jam-c-antibody_af1213" title="JAM-C was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Goat Anti-Mouse JAM-C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1213) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to muscle cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="cust_bought" > <img loading="lazy" src="https://images.novusbio.com/images2/JAM-C_AF1213_Immunohistochemistry_10165.jpg" title="JAM-C was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Goat Anti-Mouse JAM-C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1213) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to muscle cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." alt="JAM-C was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Goat Anti-Mouse JAM-C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1213) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to muscle cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" /> </a> </div> <div class="caption_container"><a href="/products/jam-c-antibody_af1213">JAM-C Antibody [Unconjugated]</a> <br />AF1213</div> <div style="text-align: right"><button class="greybutton" id="novusviewdatasheet" name="novusviewdatasheet" onclick="window.location='/products/jam-c-antibody_af1213';" >View Datasheet</button> </div> </div><div class="big_thumb_container" data-catnums="NB110-89474"> <div class="img_container"> <a href="/products/cd11b-antibody_nb110-89474" title="Immunocytochemistry/Immunofluorescence: CD11b Antibody - BSA Free [NB110-89474] - Raw264.7 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD11b Antibody conjugated to Alexa Fluor 488 (NB110-89474AF488) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved." class="cust_bought" > <img loading="lazy" src="https://images.novusbio.com/images/CD11b-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB110-89474-img0028.jpg" title="Immunocytochemistry/Immunofluorescence: CD11b Antibody - BSA Free [NB110-89474] - Raw264.7 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD11b Antibody conjugated to Alexa Fluor 488 (NB110-89474AF488) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved." alt="Immunocytochemistry/Immunofluorescence: CD11b Antibody - BSA Free [NB110-89474] - Raw264.7 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD11b Antibody conjugated to Alexa Fluor 488 (NB110-89474AF488) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved." class="big_thumb" /> </a> </div> <div class="caption_container"><a href="/products/cd11b-antibody_nb110-89474">CD11b Antibody - BSA Free</a> <br />NB110-89474</div> <div style="text-align: right"><button class="greybutton" id="novusviewdatasheet" name="novusviewdatasheet" onclick="window.location='/products/cd11b-antibody_nb110-89474';" >View Datasheet</button> </div> </div></div></div></div><div class="clear"></div><a id="jump_distributor"></a> <h2 class="greyHead2">Contact Information</h2> <div class="ds_info"> <div id="distributors"></div> </div> <div id="et_reload_trigger" style="display:none;">reload</div><h2 class="greyHead2">Product PDFs</h2><div class="silverHeadShort"> <span class="file"><a href="//aero.bio-techne.com/sds/pdf/1213-j3" target="_blank" class="sprite_dsPDF"><span class="pdfSmaller"> </span></a></span> <span class="download-file-text" ><a href="//aero.bio-techne.com/sds/pdf/1213-j3" target="_blank" class="datasheet-download sdsFile">PDF SDS</a></span> </div><div class="silverHeadShort"> <span class="file"><a href="//resources.rndsystems.com/pdfs/datasheets/1213-j3.pdf" target="_blank" class="sprite_dsPDF"><span class="pdfSmaller"> </span></a></span> <span class="download-file-text" ><a href="//resources.rndsystems.com/pdfs/datasheets/1213-j3.pdf" target="_blank" class="datasheet-download">PDF Datasheet</a></span> </div><div class="silverHeadShort"> <span class="file"><a href="mailto:novus@novusbio.com?subject=Request%20CofA%20for%201213-J3" class="sprite_newDocument"><span class="downloadBlank"> </span></a></span> <span class="download-file-text"><a href="mailto:nb-technical@bio-techne.com?subject=Request%20CofA%20for%201213-J3&body=Specific%20catalog%20number(if multiple sizes):%0ALot%20Number:%0AAny%20additional%20products:">Request CofA</a></span> </div><h2 class="greyHead2">Calculators</h2> <div class="calc_wrapper"> <div class="dsSprite_calc"></div> <div class="fake_link" id="calc_reconstitution_link">Concentration Calculator</div> <div id="calc_reconstitution_wrapper" class="calc_function_wrapper"> <div class="calc_popup"> <div class="calc_close"> <div class="dsSprite_calc_close"></div> </div> <div class="calc_header">Concentration Calculator</div> <div id="reconstitution_calculator" class="calculator"> <div class="reconstitution_calculator_text" id="reconstitution_calculator_instructions"> <p>The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. 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Recombinant Mouse JAM-C Fc Chimera Protein, CF (1213-J3): Novus Biologicals