Tight Junction Protein 1 Antibody (NBP1-85047): Novus Biologicals

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View Rabbit Polyclonal anti-Tight Junction Protein 1 Antibody (NBP1-85047). Validated Applications: WB, Simple Western, ICC/IF, IHC. Validated Species: Human, Mouse, Rat + more. Validated by: Biological Strategies, Genetic Strategies, Orthogonal Strategies." /> <meta property="og:description" content="Cited in 20 publications. View Rabbit Polyclonal anti-Tight Junction Protein 1 Antibody (NBP1-85047). Validated Applications: WB, Simple Western, ICC/IF, IHC. Validated Species: Human, Mouse, Rat + more. 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Primary human renal TEC pre-incubated with different doses of 3HK or 3HAA overnight; the cells are challenged with the cytokine cocktail (15 nM IFN gamma , 6 nM TNF alpha , & 3 nM IL1 beta ) for 24\u00a0h; protein expression was assayed with Western blot: (A, B) 3HK & 3HAA cannot reverse IDO, MHC I, BID expression induced by cytokines. 3HK & 3HAA effectively restore Bcl-xL & Tjp1 expression. 3HAA shows its different function in up-regulation of AhR expression in TEC in inflammatory conditions. (C) Qualification of protein expression in TEC challenged with cytokine cocktail in the presence of 3HK. ***P < 0.0001 versus cell treated with Cyto. (D) Qualification of protein expression in TEC challenged with cytokine cocktail in the presence of 3HAA. ***P < 0.0001, ****P < 0.00001 versus cell treated with Cyto. Pairwise comparison of individual dose to control indicated same p-value. All Western blots are representative of three independent experiments; analysis is multiple one-way ANOVA. 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(C) Qualification of protein expression in TEC challenged with cytokine cocktail in the presence of 3HK. ***P < 0.0001 versus cell treated with Cyto. (D) Qualification of protein expression in TEC challenged with cytokine cocktail in the presence of 3HAA. ***P < 0.0001, ****P < 0.00001 versus cell treated with Cyto. Pairwise comparison of individual dose to control indicated same p-value. All Western blots are representative of three independent experiments; analysis is multiple one-way ANOVA. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34305900), licensed under a CC-BY license. 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Protein 1 Antibody</div> </div> <div class="main grid_16">  <div class="region region-banners"> <div id="block-block-26" class="block block-block"> <div class="content"> <div class="container_12"><a href="https://www.bio-techne.com/account/novus-bio-techne/login?_ga=2.17257593.151271452<br />
5.1724696046-473375462.1712005244" style="margin: 0 10px;" target="_blank"><img alt="Novus Biologicals products are now on bio-techne.com" src="https://images.novusbio.com/design/ready-to-buy-novus-products-login.png" style="height:120px; width:940px" /></a></div> </div> </div> </div> <h1>Tight Junction Protein 1 Antibody</h1> </div> <div class="clear"></div> <div> <div class="region region-content"> <div id="block-et-messages-et-messages-block" class="block block-et-messages"> <div class="content"> <span></span> </div> </div> <div id="block-system-main" class="block block-system"> <div class="content"> <div class="grid_16"><div class="ds_info float_left"><a href="#PublicationSection"><div class="sprite_pubBook_large"></div></a> <a href="#PublicationSection" class="bottom2"><span class="ctr">(20 Publications)</span></a></div><div class="ds_info float_left"><a href="#ReviewsSection"><div class="dsSprite_stars5"></div></a> <a href="#ReviewsSection" class="bottom0"><span class="ctr">(5 Reviews)</span></a></div><div class="ds_info float_left"><a href="/proteinsimple" title="Learn more about Simple Western"><div class="sprite_swBadge simple"> </div></a></div><div class="ds_info float_left"> <a href="/common-name/tight-junction-protein-1"><div class="sprite_allRelated"></div></a> <a href="/common-name/tight-junction-protein-1" class="bottom4"><span class="ctr">See All Related</span></a> </div><div class="ds_info float_left"><a href="/reviews/NBP1-85047"><div class="dsSprite_submitreview"></div></a> <a href="/reviews/NBP1-85047" class="bottom4"><span class="ctr">Submit a Review</span></a></div></div></div><div class="modalWrapper" style="display:none;"> <h2>Species Glossary</h2> <hr class="customhr"/> <img src="//images.novusbio.com/design/bunny.gif" alt="Species Glossary Rabbit" style="float: right;"> <table class="modalTable"> <tr> <td>At</td> <td>=</td> <td>A. thaliana</td> <td>SyHa</td> <td>=</td> <td>Golden Syrian Hamster</td> </tr> <tr> <td>All</td> <td>=</td> <td>All Species</td> <td>Ha</td> <td>=</td> <td>Hamster</td> </tr> <tr> <td>An</td> <td>=</td> <td>Animal</td> <td>Hu</td> <td>=</td> <td>Human</td> </tr> <tr> <td>ArHa</td> <td>=</td> <td>Armenian Hamster</td> <td>I</td> <td>=</td> <td>Insect</td> </tr> <tr> <td>Av</td> <td>=</td> <td>Avian</td> <td>Ll</td> <td>=</td> <td>Llama</td> </tr> <tr> <td>Bb</td> <td>=</td> <td>Baboon</td> <td>Ma</td> <td>=</td> <td>Mammal</td> </tr> <tr> <td>Ba</td> <td>=</td> <td>Bacteria</td> <td>Mk</td> <td>=</td> <td>Monkey</td> </tr> <tr> <td>Bv</td> <td>=</td> <td>Bovine</td> <td>Mu</td> <td>=</td> <td>Mouse</td> </tr> <tr> <td>Ca</td> <td>=</td> <td>Canine</td> <td>Multi</td> <td>=</td> <td>Multi-species</td> </tr> <tr> <td>Ce</td> <td>=</td> <td>C. Elegans</td> <td>NA</td> <td>=</td> <td>Non-species specific</td> </tr> <tr> <td>Ch</td> <td>=</td> <td>Chicken</td> <td>Or</td> <td>=</td> <td>Orangutan</td> </tr> <tr> <td>ChHa</td> <td>=</td> <td>Chinese Hamster</td> <td>Pl</td> <td>=</td> <td>Plant</td> </tr> <tr> <td>Cm</td> <td>=</td> <td>Cynomolgus Monkey</td> <td>Pm</td> <td>=</td> <td>Primate</td> </tr> <tr> <td>Do</td> <td>=</td> <td>Donkey</td> <td>Po</td> <td>=</td> <td>Porcine</td> </tr> <tr> <td>Dr</td> <td>=</td> <td>Drosophilia</td> <td>Rb</td> <td>=</td> <td>Rabbit</td> </tr> <tr> <td>Ec</td> <td>=</td> <td>E. Coli</td> <td>Rt</td> <td>=</td> <td>Rat</td> </tr> <tr> <td>Eq</td> <td>=</td> <td>Equine</td> <td>RM</td> <td>=</td> <td>Rhesus Macaque</td> </tr> <tr> <td>Fe</td> <td>=</td> <td>Feline</td> <td>Sh</td> <td>=</td> <td>Sheep</td> </tr> <tr> <td>Ft</td> <td>=</td> <td>Ferret</td> <td>Vb</td> <td>=</td> <td>Vertebrate</td> </tr> <tr> <td>Fi</td> <td>=</td> <td>Fish</td> <td>Vi</td> <td>=</td> <td>Virus</td> </tr> <tr> <td>Fu</td> <td>=</td> <td>Fungi</td> <td>Xp</td> <td>=</td> <td>Xenopus</td> </tr> <tr> <td>Ge</td> <td>=</td> <td>Gerbil</td> <td>Ye</td> <td>=</td> <td>Yeast</td> </tr> <tr> <td>GP</td> <td>=</td> <td>Guinea Pig</td> <td>Ze</td> <td>=</td> <td>Zebrafish</td> </tr> <tr> <td>Gt</td> <td>=</td> <td>Goat</td> </tr> </table> <div style="text-align: center;margin-left:25px;margin-right:25px;"> <p>Note: Mouseover a species abbreviation on the product page to display the fullname.</p> </div> </div> </div><div class="clear"></div><div class="grid_10 pos_relative"><div class="ds_blue_border equaltop"><div class="imagesleft equaltop"><h2 class="blueHead">Images</h2> <div class="midBlue"> </div><div class="images"><div id="ss_prev_top" class="cycle-pager"><div class="pager_left_on"></div></div><div class="cycle-slideshow slideshows" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-top" data-cycle-prev="#ss_prev_top" data-cycle-next="#ss_next_top" data-cycle-caption="#image-caption" data-cycle-caption-template="Image {{slideNum}} of {{slideCount}}" data-cycle-slides=".big_thumb_container" data-cycle-carousel-visible="5" id="images_cycle" > <div class="sub_image_wrapper"><div id="cycle-circle-pager-top" class="cycle-circle-pager"></div><div id="image-caption"></div> <a class="enlargeTop">(<div class="dsSprite_magGlass"></div> Enlarge)</a></div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-Knockout-Validated-NBP1-85047-img0019.jpg" class="top_images" rel="gallery" title="<b>Genetic Strategies Validation. </b>Knockout Validated: Tight Junction Protein 1 Antibody [NBP1-85047] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and TJP1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:200 of Rabbit Anti-Human TJP1 Polyclonal Antibody (Catalog # NBP1-85047) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for TJP1 at approximately 260 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions." > <img src="//images.novusbio.com/images/Tight-Junction-Protein-1-Antibody-Knockout-Validated-NBP1-85047-img0019.jpg" class="big_thumb" alt="Knockout Validated: Tight Junction Protein 1 Antibody [NBP1-85047] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and TJP1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:200 of Rabbit Anti-Human TJP1 Polyclonal Antibody (Catalog # NBP1-85047) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for TJP1 at approximately 260 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions." title="Knockout Validated: Tight Junction Protein 1 Antibody [NBP1-85047] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and TJP1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:200 of Rabbit Anti-Human TJP1 Polyclonal Antibody (Catalog # NBP1-85047) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for TJP1 at approximately 260 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions." /> </a> </div> <div class="alt-caption padding_left5" ><b>Genetic Strategies</b>: Knockout Validated: Tight Junction Protein 1 Antibody [NBP1-85047] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and TJP1 knockout (KO) HeLa cell<a class="openGallery" href="#" data-picnum="0"> ...read more</a></div> </div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85047-img0018.jpg" class="top_images" rel="gallery" title="<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Tight Junction Protein 1 Antibody [NBP1-85047] - Staining in human testis and skeletal muscle tissues. Corresponding TJP1 RNA-seq data are presented for the same tissues." > <img src="//images.novusbio.com/images/Tight-Junction-Protein-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85047-img0018.jpg" loading="lazy" style="display:none;" class="big_thumb" alt="Immunohistochemistry-Paraffin: Tight Junction Protein 1 Antibody [NBP1-85047] - Staining in human testis and skeletal muscle tissues. Corresponding TJP1 RNA-seq data are presented for the same tissues." title="Immunohistochemistry-Paraffin: Tight Junction Protein 1 Antibody [NBP1-85047] - Staining in human testis and skeletal muscle tissues. Corresponding TJP1 RNA-seq data are presented for the same tissues." /> </a> </div> <div class="alt-caption padding_left5" ><b>Orthogonal Strategies</b>: Immunohistochemistry-Paraffin: Tight Junction Protein 1 Antibody [NBP1-85047] - Staining in human testis and skeletal muscle tissues. Corresponding TJP1 RNA-seq data are presented for the<a class="openGallery" href="#" data-picnum="1"> ...read more</a></div> </div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-Simple-Western-NBP1-85047-img0006.jpg" class="top_images" rel="gallery" title="Simple Western: Tight Junction Protein 1 Antibody [NBP1-85047] - Simple Western lane view shows a specific band for TJP1 in 0.2 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 66-440 kDa separation system." > <img src="//images.novusbio.com/images/Tight-Junction-Protein-1-Antibody-Simple-Western-NBP1-85047-img0006.jpg" loading="lazy" style="display:none;" class="big_thumb" alt="Simple Western: Tight Junction Protein 1 Antibody [NBP1-85047] - Simple Western lane view shows a specific band for TJP1 in 0.2 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 66-440 kDa separation system." title="Simple Western: Tight Junction Protein 1 Antibody [NBP1-85047] - Simple Western lane view shows a specific band for TJP1 in 0.2 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 66-440 kDa separation system." /> </a> </div> <div class="alt-caption padding_left5" >Simple Western: Tight Junction Protein 1 Antibody [NBP1-85047] - Simple Western lane view shows a specific band for TJP1 in 0.2 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the<a class="openGallery" href="#" data-picnum="2"> ...read more</a></div> </div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-Immunohistochemistry-NBP1-85047-img0025.jpg" class="top_images" rel="gallery" title="Immunohistochemistry: Tight Junction Protein 1 Antibody [NBP1-85047] - Representative images of DCFA excretion at the biliary poles of corr-FH-iHeps. Left image FH-i, right image Corr-FH-iHeps. All images were taken with 10x objective, z-stacks of xy sections of the cells were acquired with an epifluorescence microscope (Nikon Elipse) and analyzed with ImageJ software. Image collected and cropped by CiteAb from the following publication (//stemcellres.biomedcentral.com/articles/10.1186/s13287-019-1342-6) licensed under a CC-BY license." > <img src="//images.novusbio.com/images/Tight-Junction-Protein-1-Antibody-Immunohistochemistry-NBP1-85047-img0025.jpg" loading="lazy" style="display:none;" class="big_thumb" alt="Immunohistochemistry: Tight Junction Protein 1 Antibody [NBP1-85047] - Representative images of DCFA excretion at the biliary poles of corr-FH-iHeps. Left image FH-i, right image Corr-FH-iHeps. All images were taken with 10x objective, z-stacks of xy sections of the cells were acquired with an epifluorescence microscope (Nikon Elipse) and analyzed with ImageJ software. Image collected and cropped by CiteAb from the following publication (//stemcellres.biomedcentral.com/articles/10.1186/s13287-019-1342-6) licensed under a CC-BY license." title="Immunohistochemistry: Tight Junction Protein 1 Antibody [NBP1-85047] - Representative images of DCFA excretion at the biliary poles of corr-FH-iHeps. Left image FH-i, right image Corr-FH-iHeps. All images were taken with 10x objective, z-stacks of xy sections of the cells were acquired with an epifluorescence microscope (Nikon Elipse) and analyzed with ImageJ software. Image collected and cropped by CiteAb from the following publication (//stemcellres.biomedcentral.com/articles/10.1186/s13287-019-1342-6) licensed under a CC-BY license." /> </a> </div> <div class="alt-caption padding_left5" >Immunohistochemistry: Tight Junction Protein 1 Antibody [NBP1-85047] - Representative images of DCFA excretion at the biliary poles of corr-FH-iHeps. Left image FH-i, right image Corr-FH-iHeps. All images were taken<a class="openGallery" href="#" data-picnum="3"> ...read more</a></div> </div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-Western-Blot-NBP1-85047-img0026.jpg" class="top_images" rel="gallery" title="<b>Biological Strategies Validation. </b>Western Blot: Tight Junction Protein 1 Antibody [NBP1-85047] - Effects of dietary Flammulina velutipes stem waste (FVS) inclusion on the relative expression of tight junction proteins in jejunum of growing pigs. a The relative expression of ZO-1 in jejunum of growing pigs fed control and 2.5% or 5% FVS diets. Values are means (3 pigs per treatment) with standard errors represented by vertical bars. *P < 0.05, **P < 0.01. Image collected and cropped by CiteAb from the following publication (//jasbsci.biomedcentral.com/articles/10.1186/s40104-020-00449-y) licensed under a CC-BY license." > <img src="//images.novusbio.com/images/Tight-Junction-Protein-1-Antibody-Western-Blot-NBP1-85047-img0026.jpg" loading="lazy" style="display:none;" class="big_thumb" alt="Western Blot: Tight Junction Protein 1 Antibody [NBP1-85047] - Effects of dietary Flammulina velutipes stem waste (FVS) inclusion on the relative expression of tight junction proteins in jejunum of growing pigs. a The relative expression of ZO-1 in jejunum of growing pigs fed control and 2.5% or 5% FVS diets. Values are means (3 pigs per treatment) with standard errors represented by vertical bars. *P < 0.05, **P < 0.01. Image collected and cropped by CiteAb from the following publication (//jasbsci.biomedcentral.com/articles/10.1186/s40104-020-00449-y) licensed under a CC-BY license." title="Western Blot: Tight Junction Protein 1 Antibody [NBP1-85047] - Effects of dietary Flammulina velutipes stem waste (FVS) inclusion on the relative expression of tight junction proteins in jejunum of growing pigs. a The relative expression of ZO-1 in jejunum of growing pigs fed control and 2.5% or 5% FVS diets. Values are means (3 pigs per treatment) with standard errors represented by vertical bars. *P < 0.05, **P < 0.01. Image collected and cropped by CiteAb from the following publication (//jasbsci.biomedcentral.com/articles/10.1186/s40104-020-00449-y) licensed under a CC-BY license." /> </a> </div> <div class="alt-caption padding_left5" ><b>Biological Strategies</b>: Western Blot: Tight Junction Protein 1 Antibody [NBP1-85047] - Effects of dietary Flammulina velutipes stem waste (FVS) inclusion on the relative expression of tight junction proteins in<a class="openGallery" href="#" data-picnum="4"> ...read more</a></div> </div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85047-img0024.jpg" class="top_images" rel="gallery" title="Immunocytochemistry/Immunofluorescence: Tight Junction Protein 1 Antibody [NBP1-85047] - Rat epithelial cells stained positively with Tight Junction Protein 1. Primary antibody at 1:100. Secondary antibody: goat anti-rabbit-Alexa 488 conjugated at 1:500. ICC/IF image submitted by a verified customer review." > <img src="//images.novusbio.com/images/Tight-Junction-Protein-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85047-img0024.jpg" loading="lazy" style="display:none;" class="big_thumb" alt="Immunocytochemistry/Immunofluorescence: Tight Junction Protein 1 Antibody [NBP1-85047] - Rat epithelial cells stained positively with Tight Junction Protein 1. Primary antibody at 1:100. Secondary antibody: goat anti-rabbit-Alexa 488 conjugated at 1:500. ICC/IF image submitted by a verified customer review." title="Immunocytochemistry/Immunofluorescence: Tight Junction Protein 1 Antibody [NBP1-85047] - Rat epithelial cells stained positively with Tight Junction Protein 1. Primary antibody at 1:100. Secondary antibody: goat anti-rabbit-Alexa 488 conjugated at 1:500. ICC/IF image submitted by a verified customer review." /> </a> </div> <div class="alt-caption padding_left5" >Immunocytochemistry/ Immunofluorescence: Tight Junction Protein 1 Antibody [NBP1-85047] - Rat epithelial cells stained positively with Tight Junction Protein 1. Primary antibody at 1:100. Secondary antibody: goat<a class="openGallery" href="#" data-picnum="5"> ...read more</a></div> </div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-Western-Blot-NBP1-85047-img0020.jpg" class="top_images" rel="gallery" title="Western Blot: Tight Junction Protein 1 Antibody [NBP1-85047] - Analysis in control (vector only transfected HEK293T lysate) and TJP1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells)." > <img src="//images.novusbio.com/images/Tight-Junction-Protein-1-Antibody-Western-Blot-NBP1-85047-img0020.jpg" loading="lazy" style="display:none;" class="big_thumb" alt="Western Blot: Tight Junction Protein 1 Antibody [NBP1-85047] - Analysis in control (vector only transfected HEK293T lysate) and TJP1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells)." title="Western Blot: Tight Junction Protein 1 Antibody [NBP1-85047] - Analysis in control (vector only transfected HEK293T lysate) and TJP1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells)." /> </a> </div> <div class="alt-caption padding_left5" >Western Blot: Tight Junction Protein 1 Antibody [NBP1-85047] - Analysis in control (vector only transfected HEK293T lysate) and TJP1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in<a class="openGallery" href="#" data-picnum="6"> ...read more</a></div> </div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-Western-Blot-NBP1-85047-img0021.jpg" class="top_images" rel="gallery" title="Western Blot: Tight Junction Protein 1 Antibody [NBP1-85047] - Analysis in human cell line A-431." > <img src="//images.novusbio.com/images/Tight-Junction-Protein-1-Antibody-Western-Blot-NBP1-85047-img0021.jpg" loading="lazy" style="display:none;" class="big_thumb" alt="Western Blot: Tight Junction Protein 1 Antibody [NBP1-85047] - Analysis in human cell line A-431." title="Western Blot: Tight Junction Protein 1 Antibody [NBP1-85047] - Analysis in human cell line A-431." /> </a> </div> <div class="alt-caption padding_left5" >Western Blot: Tight Junction Protein 1 Antibody [NBP1-85047] - Analysis in human cell line A-431.</div> </div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85047-img0013.jpg" class="top_images" rel="gallery" title="Immunocytochemistry/Immunofluorescence: Tight Junction Protein 1 Antibody [NBP1-85047] - Staining of Pig trabecular meshwork cells. ICC/IF image submitted by a verified customer review." > <img src="//images.novusbio.com/images/Tight-Junction-Protein-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85047-img0013.jpg" loading="lazy" style="display:none;" class="big_thumb" alt="Immunocytochemistry/Immunofluorescence: Tight Junction Protein 1 Antibody [NBP1-85047] - Staining of Pig trabecular meshwork cells. ICC/IF image submitted by a verified customer review." title="Immunocytochemistry/Immunofluorescence: Tight Junction Protein 1 Antibody [NBP1-85047] - Staining of Pig trabecular meshwork cells. ICC/IF image submitted by a verified customer review." /> </a> </div> <div class="alt-caption padding_left5" >Immunocytochemistry/ Immunofluorescence: Tight Junction Protein 1 Antibody [NBP1-85047] - Staining of Pig trabecular meshwork cells. ICC/IF image submitted by a verified customer review.</div> </div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85047-img0008.jpg" class="top_images" rel="gallery" title="Immunocytochemistry/Immunofluorescence: Tight Junction Protein 1 Antibody [NBP1-85047] - Staining of human cell line U-2 OS shows localization to cytosol & cell junctions. Antibody staining is shown in green." > <img src="//images.novusbio.com/images/Tight-Junction-Protein-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85047-img0008.jpg" loading="lazy" style="display:none;" class="big_thumb" alt="Immunocytochemistry/Immunofluorescence: Tight Junction Protein 1 Antibody [NBP1-85047] - Staining of human cell line U-2 OS shows localization to cytosol & cell junctions. Antibody staining is shown in green." title="Immunocytochemistry/Immunofluorescence: Tight Junction Protein 1 Antibody [NBP1-85047] - Staining of human cell line U-2 OS shows localization to cytosol & cell junctions. Antibody staining is shown in green." /> </a> </div> <div class="alt-caption padding_left5" >Immunocytochemistry/ Immunofluorescence: Tight Junction Protein 1 Antibody [NBP1-85047] - Staining of human cell line U-2 OS shows localization to cytosol & cell junctions. Antibody staining is shown in green.</div> </div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85047-img0022.jpg" class="top_images" rel="gallery" title="Immunocytochemistry/Immunofluorescence: Tight Junction Protein 1 Antibody [NBP1-85047] - The primary mouse lung endothelial cells were fixed, permeabilized and stained with 1:100 diluted anti-ZO-1 ab for overnight. Samples were washed and subsequently incubated with 1:500 diluted Alexa Fuor 546 at room temperature for 1 hour. ICC/iF image submitted by a verified customer reveiw." > <img src="//images.novusbio.com/images/Tight-Junction-Protein-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85047-img0022.jpg" loading="lazy" style="display:none;" class="big_thumb" alt="Immunocytochemistry/Immunofluorescence: Tight Junction Protein 1 Antibody [NBP1-85047] - The primary mouse lung endothelial cells were fixed, permeabilized and stained with 1:100 diluted anti-ZO-1 ab for overnight. Samples were washed and subsequently incubated with 1:500 diluted Alexa Fuor 546 at room temperature for 1 hour. ICC/iF image submitted by a verified customer reveiw." title="Immunocytochemistry/Immunofluorescence: Tight Junction Protein 1 Antibody [NBP1-85047] - The primary mouse lung endothelial cells were fixed, permeabilized and stained with 1:100 diluted anti-ZO-1 ab for overnight. Samples were washed and subsequently incubated with 1:500 diluted Alexa Fuor 546 at room temperature for 1 hour. ICC/iF image submitted by a verified customer reveiw." /> </a> </div> <div class="alt-caption padding_left5" >Immunocytochemistry/ Immunofluorescence: Tight Junction Protein 1 Antibody [NBP1-85047] - The primary mouse lung endothelial cells were fixed, permeabilized and stained with 1:100 diluted anti-ZO-1 ab for overnight.<a class="openGallery" href="#" data-picnum="10"> ...read more</a></div> </div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85047-img0023.jpg" class="top_images" rel="gallery" title="Immunocytochemistry/Immunofluorescence: Tight Junction Protein 1 Antibody [NBP1-85047] - Monolayer of Human ARPE-19 cells on a glass substrate. Tight Junction Protein 1 staining in red. ICC/IF image submitted by a verified customer review." > <img src="//images.novusbio.com/images/Tight-Junction-Protein-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85047-img0023.jpg" loading="lazy" style="display:none;" class="big_thumb" alt="Immunocytochemistry/Immunofluorescence: Tight Junction Protein 1 Antibody [NBP1-85047] - Monolayer of Human ARPE-19 cells on a glass substrate. Tight Junction Protein 1 staining in red. ICC/IF image submitted by a verified customer review." title="Immunocytochemistry/Immunofluorescence: Tight Junction Protein 1 Antibody [NBP1-85047] - Monolayer of Human ARPE-19 cells on a glass substrate. Tight Junction Protein 1 staining in red. ICC/IF image submitted by a verified customer review." /> </a> </div> <div class="alt-caption padding_left5" >Immunocytochemistry/ Immunofluorescence: Tight Junction Protein 1 Antibody [NBP1-85047] - Monolayer of Human ARPE-19 cells on a glass substrate. Tight Junction Protein 1 staining in red. ICC/IF image submitted by a<a class="openGallery" href="#" data-picnum="11"> ...read more</a></div> </div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85047-img0014.jpg" class="top_images" rel="gallery" title="Immunohistochemistry-Paraffin: Tight Junction Protein 1 Antibody [NBP1-85047] - Staining of human kidney shows strong membranous positivity in cells in glomeruli." > <img src="//images.novusbio.com/images/Tight-Junction-Protein-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85047-img0014.jpg" loading="lazy" style="display:none;" class="big_thumb" alt="Immunohistochemistry-Paraffin: Tight Junction Protein 1 Antibody [NBP1-85047] - Staining of human kidney shows strong membranous positivity in cells in glomeruli." title="Immunohistochemistry-Paraffin: Tight Junction Protein 1 Antibody [NBP1-85047] - Staining of human kidney shows strong membranous positivity in cells in glomeruli." /> </a> </div> <div class="alt-caption padding_left5" >Immunohistochemistry-Paraffin: Tight Junction Protein 1 Antibody [NBP1-85047] - Staining of human kidney shows strong membranous positivity in cells in glomeruli.</div> </div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85047-img0015.jpg" class="top_images" rel="gallery" title="Immunohistochemistry-Paraffin: Tight Junction Protein 1 Antibody [NBP1-85047] - Staining of human placenta shows moderate membranous positivity in trophoblastic cells." > <img src="//images.novusbio.com/images/Tight-Junction-Protein-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85047-img0015.jpg" loading="lazy" style="display:none;" class="big_thumb" alt="Immunohistochemistry-Paraffin: Tight Junction Protein 1 Antibody [NBP1-85047] - Staining of human placenta shows moderate membranous positivity in trophoblastic cells." title="Immunohistochemistry-Paraffin: Tight Junction Protein 1 Antibody [NBP1-85047] - Staining of human placenta shows moderate membranous positivity in trophoblastic cells." /> </a> </div> <div class="alt-caption padding_left5" >Immunohistochemistry-Paraffin: Tight Junction Protein 1 Antibody [NBP1-85047] - Staining of human placenta shows moderate membranous positivity in trophoblastic cells.</div> </div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85047-img0016.jpg" class="top_images" rel="gallery" title="Immunohistochemistry-Paraffin: Tight Junction Protein 1 Antibody [NBP1-85047] - Staining of human skeletal muscle shows no membranous positivity in myocytes as expected." > <img src="//images.novusbio.com/images/Tight-Junction-Protein-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85047-img0016.jpg" loading="lazy" style="display:none;" class="big_thumb" alt="Immunohistochemistry-Paraffin: Tight Junction Protein 1 Antibody [NBP1-85047] - Staining of human skeletal muscle shows no membranous positivity in myocytes as expected." title="Immunohistochemistry-Paraffin: Tight Junction Protein 1 Antibody [NBP1-85047] - Staining of human skeletal muscle shows no membranous positivity in myocytes as expected." /> </a> </div> <div class="alt-caption padding_left5" >Immunohistochemistry-Paraffin: Tight Junction Protein 1 Antibody [NBP1-85047] - Staining of human skeletal muscle shows no membranous positivity in myocytes as expected.</div> </div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85047-img0017.jpg" class="top_images" rel="gallery" title="Immunohistochemistry-Paraffin: Tight Junction Protein 1 Antibody [NBP1-85047] - Staining of human testis shows moderate to strong membranous positivity in cells in seminiferous ducts." > <img src="//images.novusbio.com/images/Tight-Junction-Protein-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85047-img0017.jpg" loading="lazy" style="display:none;" class="big_thumb" alt="Immunohistochemistry-Paraffin: Tight Junction Protein 1 Antibody [NBP1-85047] - Staining of human testis shows moderate to strong membranous positivity in cells in seminiferous ducts." title="Immunohistochemistry-Paraffin: Tight Junction Protein 1 Antibody [NBP1-85047] - Staining of human testis shows moderate to strong membranous positivity in cells in seminiferous ducts." /> </a> </div> <div class="alt-caption padding_left5" >Immunohistochemistry-Paraffin: Tight Junction Protein 1 Antibody [NBP1-85047] - Staining of human testis shows moderate to strong membranous positivity in cells in seminiferous ducts.</div> </div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize/nbp1-85047_rabbit-polyclonal-tight-junction-protein-1-antibody-310202415384165.jpg" class="top_images" rel="gallery" title="Western Blot: Tight Junction Protein 1 Antibody [NBP1-85047] - Western blot analysis of function protein expression on primary human renal cortical epithelial cells challenged with cytokines. Primary human renal tubular epithelial cells are challenged with cytokine cocktail (15nM IFN gamma , 6nM TNF alpha , & 3nM IL1 beta ). (A, B) Expression of Snail, E-Cad, TJP1, SMA. AhR, IDO, KMO, KY, MHCI & II. (C, D) Densitometric quantitation of protein in (A, B). All Western blots are representative of at least three independent experiments. *P < 0.05, ***P < 0.0001 versus to cells without treatment, analysis is multiple t-tests, pairwise comparison of individual time-point to control indicated same p-value. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34305900), licensed under a CC-BY license. Not internally tested by Novus Biologicals." > <img src="//images.novusbio.com/images/nbp1-85047_rabbit-polyclonal-tight-junction-protein-1-antibody-310202415384165.jpg" loading="lazy" style="display:none;" class="big_thumb" alt="Western Blot: Tight Junction Protein 1 Antibody [NBP1-85047] - Western blot analysis of function protein expression on primary human renal cortical epithelial cells challenged with cytokines. Primary human renal tubular epithelial cells are challenged with cytokine cocktail (15nM IFN gamma , 6nM TNF alpha , & 3nM IL1 beta ). (A, B) Expression of Snail, E-Cad, TJP1, SMA. AhR, IDO, KMO, KY, MHCI & II. (C, D) Densitometric quantitation of protein in (A, B). All Western blots are representative of at least three independent experiments. *P < 0.05, ***P < 0.0001 versus to cells without treatment, analysis is multiple t-tests, pairwise comparison of individual time-point to control indicated same p-value. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34305900), licensed under a CC-BY license. Not internally tested by Novus Biologicals." title="Western Blot: Tight Junction Protein 1 Antibody [NBP1-85047] - Western blot analysis of function protein expression on primary human renal cortical epithelial cells challenged with cytokines. Primary human renal tubular epithelial cells are challenged with cytokine cocktail (15nM IFN gamma , 6nM TNF alpha , & 3nM IL1 beta ). (A, B) Expression of Snail, E-Cad, TJP1, SMA. AhR, IDO, KMO, KY, MHCI & II. (C, D) Densitometric quantitation of protein in (A, B). All Western blots are representative of at least three independent experiments. *P < 0.05, ***P < 0.0001 versus to cells without treatment, analysis is multiple t-tests, pairwise comparison of individual time-point to control indicated same p-value. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34305900), licensed under a CC-BY license. Not internally tested by Novus Biologicals." /> </a> </div> <div class="alt-caption padding_left5" >Western Blot: Tight Junction Protein 1 Antibody [NBP1-85047] - Western blot analysis of function protein expression on primary human renal cortical epithelial cells challenged with cytokines. Primary human renal tubular<a class="openGallery" href="#" data-picnum="16"> ...read more</a></div> </div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize/nbp1-85047_rabbit-polyclonal-tight-junction-protein-1-antibody-310202415395958.jpg" class="top_images" rel="gallery" title="Western Blot: Tight Junction Protein 1 Antibody [NBP1-85047] - Effects of dietary Flammulina velutipes stem waste (FVS) inclusion on the relative expression of tight junction proteins in jejunum of growing pigs. a The relative expression of ZO-1 in jejunum of growing pigs fed control & 2.5% or 5% FVS diets. b The relative expression of occludin in jejunum of growing pigs fed control & 2.5% or 5% FVS diets. c The relative expression of claudin-1 in jejunum of growing pigs fed control & 2.5% or 5% FVS diets. Values are means (3 pigs per treatment) with standard errors represented by vertical bars. *P < 0.05, **P < 0.01 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32391146), licensed under a CC-BY license. Not internally tested by Novus Biologicals." > <img src="//images.novusbio.com/images/nbp1-85047_rabbit-polyclonal-tight-junction-protein-1-antibody-310202415395958.jpg" loading="lazy" style="display:none;" class="big_thumb" alt="Western Blot: Tight Junction Protein 1 Antibody [NBP1-85047] - Effects of dietary Flammulina velutipes stem waste (FVS) inclusion on the relative expression of tight junction proteins in jejunum of growing pigs. a The relative expression of ZO-1 in jejunum of growing pigs fed control & 2.5% or 5% FVS diets. b The relative expression of occludin in jejunum of growing pigs fed control & 2.5% or 5% FVS diets. c The relative expression of claudin-1 in jejunum of growing pigs fed control & 2.5% or 5% FVS diets. Values are means (3 pigs per treatment) with standard errors represented by vertical bars. *P < 0.05, **P < 0.01 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32391146), licensed under a CC-BY license. Not internally tested by Novus Biologicals." title="Western Blot: Tight Junction Protein 1 Antibody [NBP1-85047] - Effects of dietary Flammulina velutipes stem waste (FVS) inclusion on the relative expression of tight junction proteins in jejunum of growing pigs. a The relative expression of ZO-1 in jejunum of growing pigs fed control & 2.5% or 5% FVS diets. b The relative expression of occludin in jejunum of growing pigs fed control & 2.5% or 5% FVS diets. c The relative expression of claudin-1 in jejunum of growing pigs fed control & 2.5% or 5% FVS diets. Values are means (3 pigs per treatment) with standard errors represented by vertical bars. *P < 0.05, **P < 0.01 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32391146), licensed under a CC-BY license. Not internally tested by Novus Biologicals." /> </a> </div> <div class="alt-caption padding_left5" >Western Blot: Tight Junction Protein 1 Antibody [NBP1-85047] - Effects of dietary Flammulina velutipes stem waste (FVS) inclusion on the relative expression of tight junction proteins in jejunum of growing pigs. a The<a class="openGallery" href="#" data-picnum="17"> ...read more</a></div> </div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize/nbp1-85047_rabbit-polyclonal-tight-junction-protein-1-antibody-31020241539255.jpg" class="top_images" rel="gallery" title="Immunocytochemistry/ Immunofluorescence: Tight Junction Protein 1 Antibody [NBP1-85047] - Differentiation of FH- & corr-FH-iPSCs into hepatocytes. a Representative pictures of cell morphology & immunostainings of the indicated markers at day 25 of differentiation. Scale bars: 50 μm. b Representative images of DCFA excretion at the biliary poles of corr-FH-iHeps. All images were taken with × 10 objective, z-stacks of xy sections of the cells were acquired with an epifluorescence microscope (Nikon Elipse) & analyzed with ImageJ software. Arrowheads indicate bile canaliculi Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31358055), licensed under a CC-BY license. Not internally tested by Novus Biologicals." > <img src="//images.novusbio.com/images/nbp1-85047_rabbit-polyclonal-tight-junction-protein-1-antibody-31020241539255.jpg" loading="lazy" style="display:none;" class="big_thumb" alt="Immunocytochemistry/ Immunofluorescence: Tight Junction Protein 1 Antibody [NBP1-85047] - Differentiation of FH- & corr-FH-iPSCs into hepatocytes. a Representative pictures of cell morphology & immunostainings of the indicated markers at day 25 of differentiation. Scale bars: 50 μm. b Representative images of DCFA excretion at the biliary poles of corr-FH-iHeps. All images were taken with × 10 objective, z-stacks of xy sections of the cells were acquired with an epifluorescence microscope (Nikon Elipse) & analyzed with ImageJ software. Arrowheads indicate bile canaliculi Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31358055), licensed under a CC-BY license. Not internally tested by Novus Biologicals." title="Immunocytochemistry/ Immunofluorescence: Tight Junction Protein 1 Antibody [NBP1-85047] - Differentiation of FH- & corr-FH-iPSCs into hepatocytes. a Representative pictures of cell morphology & immunostainings of the indicated markers at day 25 of differentiation. Scale bars: 50 μm. b Representative images of DCFA excretion at the biliary poles of corr-FH-iHeps. All images were taken with × 10 objective, z-stacks of xy sections of the cells were acquired with an epifluorescence microscope (Nikon Elipse) & analyzed with ImageJ software. Arrowheads indicate bile canaliculi Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31358055), licensed under a CC-BY license. Not internally tested by Novus Biologicals." /> </a> </div> <div class="alt-caption padding_left5" >Immunocytochemistry/ Immunofluorescence: Tight Junction Protein 1 Antibody [NBP1-85047] - Differentiation of FH- & corr-FH-iPSCs into hepatocytes. a Representative pictures of cell morphology & immunostainings of the<a class="openGallery" href="#" data-picnum="18"> ...read more</a></div> </div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize/nbp1-85047_rabbit-polyclonal-tight-junction-protein-1-antibody-3102024161362.jpg" class="top_images" rel="gallery" title="Western Blot: Tight Junction Protein 1 Antibody [NBP1-85047] - Protective role of 3HK & 3HAA in TEC stimulated with cytokines. Primary human renal TEC pre-incubated with different doses of 3HK or 3HAA overnight; the cells are challenged with the cytokine cocktail (15 nM IFN gamma , 6 nM TNF alpha , & 3 nM IL1 beta ) for 24 h; protein expression was assayed with Western blot: (A, B) 3HK & 3HAA cannot reverse IDO, MHC I, BID expression induced by cytokines. 3HK & 3HAA effectively restore Bcl-xL & Tjp1 expression. 3HAA shows its different function in up-regulation of AhR expression in TEC in inflammatory conditions. (C) Qualification of protein expression in TEC challenged with cytokine cocktail in the presence of 3HK. ***P < 0.0001 versus cell treated with Cyto. (D) Qualification of protein expression in TEC challenged with cytokine cocktail in the presence of 3HAA. ***P < 0.0001, ****P < 0.00001 versus cell treated with Cyto. Pairwise comparison of individual dose to control indicated same p-value. All Western blots are representative of three independent experiments; analysis is multiple one-way ANOVA. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34305900), licensed under a CC-BY license. Not internally tested by Novus Biologicals." > <img src="//images.novusbio.com/images/nbp1-85047_rabbit-polyclonal-tight-junction-protein-1-antibody-3102024161362.jpg" loading="lazy" style="display:none;" class="big_thumb" alt="Western Blot: Tight Junction Protein 1 Antibody [NBP1-85047] - Protective role of 3HK & 3HAA in TEC stimulated with cytokines. Primary human renal TEC pre-incubated with different doses of 3HK or 3HAA overnight; the cells are challenged with the cytokine cocktail (15 nM IFN gamma , 6 nM TNF alpha , & 3 nM IL1 beta ) for 24 h; protein expression was assayed with Western blot: (A, B) 3HK & 3HAA cannot reverse IDO, MHC I, BID expression induced by cytokines. 3HK & 3HAA effectively restore Bcl-xL & Tjp1 expression. 3HAA shows its different function in up-regulation of AhR expression in TEC in inflammatory conditions. (C) Qualification of protein expression in TEC challenged with cytokine cocktail in the presence of 3HK. ***P < 0.0001 versus cell treated with Cyto. (D) Qualification of protein expression in TEC challenged with cytokine cocktail in the presence of 3HAA. ***P < 0.0001, ****P < 0.00001 versus cell treated with Cyto. Pairwise comparison of individual dose to control indicated same p-value. All Western blots are representative of three independent experiments; analysis is multiple one-way ANOVA. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34305900), licensed under a CC-BY license. Not internally tested by Novus Biologicals." title="Western Blot: Tight Junction Protein 1 Antibody [NBP1-85047] - Protective role of 3HK & 3HAA in TEC stimulated with cytokines. Primary human renal TEC pre-incubated with different doses of 3HK or 3HAA overnight; the cells are challenged with the cytokine cocktail (15 nM IFN gamma , 6 nM TNF alpha , & 3 nM IL1 beta ) for 24 h; protein expression was assayed with Western blot: (A, B) 3HK & 3HAA cannot reverse IDO, MHC I, BID expression induced by cytokines. 3HK & 3HAA effectively restore Bcl-xL & Tjp1 expression. 3HAA shows its different function in up-regulation of AhR expression in TEC in inflammatory conditions. (C) Qualification of protein expression in TEC challenged with cytokine cocktail in the presence of 3HK. ***P < 0.0001 versus cell treated with Cyto. (D) Qualification of protein expression in TEC challenged with cytokine cocktail in the presence of 3HAA. ***P < 0.0001, ****P < 0.00001 versus cell treated with Cyto. Pairwise comparison of individual dose to control indicated same p-value. All Western blots are representative of three independent experiments; analysis is multiple one-way ANOVA. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34305900), licensed under a CC-BY license. Not internally tested by Novus Biologicals." /> </a> </div> <div class="alt-caption padding_left5" >Western Blot: Tight Junction Protein 1 Antibody [NBP1-85047] - Protective role of 3HK & 3HAA in TEC stimulated with cytokines. Primary human renal TEC pre-incubated with different doses of 3HK or 3HAA overnight; the<a class="openGallery" href="#" data-picnum="19"> ...read more</a></div> </div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize/nbp1-85047_rabbit-polyclonal-tight-junction-protein-1-antibody-310202416114731.jpg" class="top_images" rel="gallery" title="Western Blot: Tight Junction Protein 1 Antibody [NBP1-85047] - Protective role of 3HK & 3HAA in TEC stimulated with cytokines. Primary human renal TEC pre-incubated with different doses of 3HK or 3HAA overnight; the cells are challenged with the cytokine cocktail (15 nM IFN gamma , 6 nM TNF alpha , & 3 nM IL1 beta ) for 24 h; protein expression was assayed with Western blot: (A, B) 3HK & 3HAA cannot reverse IDO, MHC I, BID expression induced by cytokines. 3HK & 3HAA effectively restore Bcl-xL & Tjp1 expression. 3HAA shows its different function in up-regulation of AhR expression in TEC in inflammatory conditions. (C) Qualification of protein expression in TEC challenged with cytokine cocktail in the presence of 3HK. ***P < 0.0001 versus cell treated with Cyto. (D) Qualification of protein expression in TEC challenged with cytokine cocktail in the presence of 3HAA. ***P < 0.0001, ****P < 0.00001 versus cell treated with Cyto. Pairwise comparison of individual dose to control indicated same p-value. All Western blots are representative of three independent experiments; analysis is multiple one-way ANOVA. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34305900), licensed under a CC-BY license. Not internally tested by Novus Biologicals." > <img src="//images.novusbio.com/images/nbp1-85047_rabbit-polyclonal-tight-junction-protein-1-antibody-310202416114731.jpg" loading="lazy" style="display:none;" class="big_thumb" alt="Western Blot: Tight Junction Protein 1 Antibody [NBP1-85047] - Protective role of 3HK & 3HAA in TEC stimulated with cytokines. Primary human renal TEC pre-incubated with different doses of 3HK or 3HAA overnight; the cells are challenged with the cytokine cocktail (15 nM IFN gamma , 6 nM TNF alpha , & 3 nM IL1 beta ) for 24 h; protein expression was assayed with Western blot: (A, B) 3HK & 3HAA cannot reverse IDO, MHC I, BID expression induced by cytokines. 3HK & 3HAA effectively restore Bcl-xL & Tjp1 expression. 3HAA shows its different function in up-regulation of AhR expression in TEC in inflammatory conditions. (C) Qualification of protein expression in TEC challenged with cytokine cocktail in the presence of 3HK. ***P < 0.0001 versus cell treated with Cyto. (D) Qualification of protein expression in TEC challenged with cytokine cocktail in the presence of 3HAA. ***P < 0.0001, ****P < 0.00001 versus cell treated with Cyto. Pairwise comparison of individual dose to control indicated same p-value. All Western blots are representative of three independent experiments; analysis is multiple one-way ANOVA. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34305900), licensed under a CC-BY license. Not internally tested by Novus Biologicals." title="Western Blot: Tight Junction Protein 1 Antibody [NBP1-85047] - Protective role of 3HK & 3HAA in TEC stimulated with cytokines. Primary human renal TEC pre-incubated with different doses of 3HK or 3HAA overnight; the cells are challenged with the cytokine cocktail (15 nM IFN gamma , 6 nM TNF alpha , & 3 nM IL1 beta ) for 24 h; protein expression was assayed with Western blot: (A, B) 3HK & 3HAA cannot reverse IDO, MHC I, BID expression induced by cytokines. 3HK & 3HAA effectively restore Bcl-xL & Tjp1 expression. 3HAA shows its different function in up-regulation of AhR expression in TEC in inflammatory conditions. (C) Qualification of protein expression in TEC challenged with cytokine cocktail in the presence of 3HK. ***P < 0.0001 versus cell treated with Cyto. (D) Qualification of protein expression in TEC challenged with cytokine cocktail in the presence of 3HAA. ***P < 0.0001, ****P < 0.00001 versus cell treated with Cyto. Pairwise comparison of individual dose to control indicated same p-value. All Western blots are representative of three independent experiments; analysis is multiple one-way ANOVA. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34305900), licensed under a CC-BY license. Not internally tested by Novus Biologicals." /> </a> </div> <div class="alt-caption padding_left5" >Western Blot: Tight Junction Protein 1 Antibody [NBP1-85047] - Protective role of 3HK & 3HAA in TEC stimulated with cytokines. Primary human renal TEC pre-incubated with different doses of 3HK or 3HAA overnight; the<a class="openGallery" href="#" data-picnum="20"> ...read more</a></div> </div></div><div id="ss_next_top" class="cycle-pager"><div class="pager_right_on"></div></div></div></div><div class="productsright pos_relative equaltop"><h2 class="blueHead">Product Details</h2> <div class="midBlue">Summary</div><table class="ds_list"><tr class="lined"> <td><strong>Reactivity</strong></td> <td><span title='Human'>Hu</span>, <span title='Mouse'>Mu</span>, <span title='Rat'>Rt</span>, <span title='Porcine'>Po</span><span class="myModal">Species Glossary</span></td> </tr><tr class="lined"> <td><strong>Applications</strong></td> <td><span title='Western Blot'>WB</span>, <span title='Simple Western'>Simple Western</span>, <span title='Immunocytochemistry/ Immunofluorescence'>ICC/IF</span>, <span title='Immunohistochemistry'>IHC</span></td> </tr><tr class="lined"> <td><strong>Clonality</strong></td> <td><div>Polyclonal</div></td> </tr><tr class="lined"> <td><strong>Host</strong></td> <td><div>Rabbit</div></td> </tr><tr class="lined"> <td><strong>Conjugate</strong></td> <td><div>Unconjugated</div></td> </tr></table></div><div class="AtlasDSDiv" style="clear:left;"><div class="AtlasDSValidatedText">Validated by: </div>  <span class="AtlasDSSpan"><a href="/5-pillars-validation" class="AtlasDSBiological" title="Biological Strategies"> </a><p><a href="/5-pillars-validation" title="Biological Strategies">Biological Strategies</a></p></span> <span class="AtlasDSSpan"><a href="/5-pillars-validation" class="AtlasDSGenetic" title="Genetic Strategies"> </a><p><a href="/5-pillars-validation" title="Genetic Strategies">Genetic Strategies</a></p></span> <span class="AtlasDSSpan"><a href="/5-pillars-validation" class="AtlasDSOrthogonal" title="Orthogonal Strategies"> </a><p><a href="/5-pillars-validation" title="Orthogonal Strategies">Orthogonal Strategies</a></p></span>  </div> </div></div><div class="grid_6"><div></div> <div class="ds_blue_border equaltop"> <h2 class="blueHead_nopadding">Order Details</h2><ul class="ds_details"><li class="add-to-cart" data-type="full" data-prodname="" data-catnum="NBP1-85047" data-catnums="NBP1-85047" data-disabled=''><noscript><form autocomplete="off" action="/products/claudin-1-antibody-1c5-d9_h00009076-m01" method="post" id="et-add-multiple-to-cart-form" accept-charset="UTF-8"><div><table class="sticky-enabled"> <thead><tr><th>Size / Catalog#</th><th>Qty</th><th>Price</th> </tr></thead> <tbody> <tr class="odd"><td><div data-msg='' class="atc_catnum">NBP1-85047</div><div class="atc_size" title="catalog # NBP1-85047">0.1 ml</div></td><td><div class="form-item form-type-textfield form-item-rows-NBP1-85047-quantity"> <input type="text" id="edit-rows-nbp1-85047-quantity" name="rows[NBP1-85047][quantity]" value="" size="60" maxlength="2" class="form-text" /> </div> </td><td><div class="price"> $549.00</div></td> </tr> <tr class="even"><td colspan="3" class="stock_row">In Stock: Ships Next Business Day</td> </tr> </tbody> </table> <input class="bulk_custom iframeBulkQuote form-submit" onClick="window.location.assign("https://www.novusbio.com/novus_quoterequest?mlt=Tight+Junction+Protein+1+Antibody&catnum=NBP1-85047"); return false;" type="submit" id="edit-bulk-request" name="op" value="Bulk Request" /><input class="add_to_cart form-submit" type="submit" id="edit-add-multiple-to-cart" name="op" value="Add To Cart " /><input type="hidden" name="form_build_id" value="form-5Fc_v7fBuHURc0h7p-XUPxOrQshAupx6zXNJVaLn6fU" /> <input type="hidden" name="form_id" value="et_add_multiple_to_cart_form" /> </div></form></noscript></li></ul><div id="shipping" class="hidden"></td></tr></div><div class="conj_form_buttons"></div><div id="shipping_statement"><span id="shipping_rate"></span></div><div class="shipping_obt_transfer"><span>Novus Biologicals is part of Bio-Techne</span><br><br><a href="https://www.bio-techne.com/p/antibodies/tight-junction-protein-1-antibody_nbp1-85047" rel="follow" class="btn btn-primary">Shop this product on bio-techne.com</a></div><br><div class="learn_more"> <a href="/guarantee"> <div class="sprite_noImage" title="Novus Biologicals, part of Bio-Techne, offers 100% Guarantee"></div> <div class="learn_more_text">Novus Biologicals, part of Bio-Techne, offers 100% Guarantee</div></a> </div><span id="conj_form" class="hidden"><tr><td colspan="4"></td></tr></span> </div> </div><div class="clear"></div><div class="grid_10"><div><div id="ds_tabs" style="display:none;"> <div class="ds_tab_active" id="dsTabs_datasheet" data-anchor_desc="datasheet">Datasheet</div> <div class="ds_tab" id="dsTabs_reviews-publications" data-anchor_desc="reviews-publications">Reviews & Publications</div> <div class="ds_tab" id="dsTabs_protocols-faqs" data-anchor_desc="protocols-faqs">Protocols & FAQs</div> <div class="ds_tab" id="dsTabs_supportresearch" data-anchor_desc="supportresearch">Support & Research</div> </div><div class="ds_tab_content equalbottom" id="content_datasheet" > <a id="datasheet"></a><h2 class=greyHead2>Tight Junction Protein 1 Antibody Summary</h2> <div class="ds_info"> <a id="tab1details"></a> <table class="ds_list wide" style="table-layout:fixed;"> <tr class="lined"> <td><strong>Immunogen</strong></td> <td><div>This antibody was developed against Recombinant Protein corresponding to amino acids: RKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVRED</div></td> </tr><tr class="lined"> <td><strong>Marker</strong></td> <td><div>Intercellular Junctions/Tight Junction Marker</div></td> </tr><tr class="lined"> <td><strong>Isotype</strong></td> <td><div>IgG</div></td> </tr><tr class="lined"> <td><strong>Clonality</strong></td> <td><div>Polyclonal</div></td> </tr><tr class="lined"> <td><strong>Host</strong></td> <td><div>Rabbit</div></td> </tr><tr class="lined"> <td><strong>Gene</strong></td> <td><div>TJP1</div></td> </tr><tr class="lined"> <td><strong>Purity</strong></td> <td><div>Immunogen affinity purified</div></td> </tr><tr class="lined"> <td><strong>Innovator's Reward</strong></td> <td><div>Test in a species/application not listed above to receive a full credit towards a future purchase.<br/><div class='text_right'><a class='ds_submit_blue ds_regularPad' href='/support/innovators-reward.html'>Learn about the Innovator's Reward</a></div></div></td> </tr> </table> </div> <h2 class="greyHead2">Applications/Dilutions</h2> <div class="ds_info"> <a id="tab1appsdiluts"></a> <table class="ds_list wide"> <tr class="lined"> <td><strong>Dilutions</strong></td> <td> <ul class="ds_noDotList"><li>Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml</li><li>Immunohistochemistry 1:200 - 1:500</li><li>Immunohistochemistry-Paraffin 1:200 - 1:500</li><li>Simple Western </li><li>Western Blot 0.04-0.4 ug/ml</li> </ul> </td> </tr><tr class="lined"> <td><strong>Application Notes</strong></td> <td><div>For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. <br/><br/>In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.</div></td> </tr> <tr class="lined"> <td><strong>Control Peptide</strong></td> <td> <table class="wide ds_altTable"><tr> <td style="width:80%"><a href="/tight-junction-protein-1-protein_nbp1-85047pep">Tight Junction Protein 1 Protein (NBP1-85047PEP)</a></td> <td class="text_right"> <div class="add-to-cart" data-type="anc_btnonly" data-catbase="NBP1-85047PEP" data-return="tight-junction-protein-1-antibody_nbp1-85047" data-produrl="tight-junction-protein-1-antibody_nbp1-85047" data-catnums="NBP1-85047PEP" ></div> </td> </tr></table> </td> </tr><tr class="lined"> <td><strong>Reviewed Applications</strong></td> <td> <table class="wide"> <tr> <td> Read <a href="#ReviewsSection">5 Reviews</a> rated 4.6<div class="dsSprite_stars5"></div> using <br/>NBP1-85047 in the following applications:<br /><br /> <ul class="information-list"><li> <div class="information-subject"><a href="#ReviewsSection" class="filter_link" data-destination="review" data-type="application" data-filter="Immunocytochemistry">Immunocytochemistry</a></div> <div class="information-data"></div> </li><li> <div class="information-subject"><a href="#ReviewsSection" class="filter_link" data-destination="review" data-type="application" data-filter="Western Blot">Western Blot</a></div> <div class="information-data"></div> </li><li> <div class="information-subject"><a href="#ReviewsSection" class="filter_link" data-destination="review" data-type="application" data-filter="Other">Other</a></div> <div class="information-data"></div> </li> </ul> </td> </tr> </table> </td> </tr><tr class="lined"> <td><strong>Publications</strong></td> <td> <table> <tr> <td>Read <a href="#PublicationSection"> Publications</a> using<br/> NBP1-85047 in the following applications:<br /><br /> <ul class="information-list"><li class="noIndent"><div class="information-subject"> <a href="#PublicationSection" class="filter_link" data-destination="publication" data-type="application" data-filter="Block/Neutralize">Block/Neutralize</a> </div><div class="information-data">1 publication</div></li><li class="noIndent"><div class="information-subject"> <a href="#PublicationSection" class="filter_link" data-destination="publication" data-type="application" data-filter="ICC/IF">ICC/IF</a> </div><div class="information-data">4 publications</div></li><li class="noIndent"><div class="information-subject"> <a href="#PublicationSection" class="filter_link" data-destination="publication" data-type="application" data-filter="IF/IHC">IF/IHC</a> </div><div class="information-data">1 publication</div></li><li class="noIndent"><div class="information-subject"> <a href="#PublicationSection" class="filter_link" data-destination="publication" data-type="application" data-filter="IHC-P">IHC-P</a> </div><div class="information-data">2 publications</div></li><li class="noIndent"><div class="information-subject"> <a href="#PublicationSection" class="filter_link" data-destination="publication" data-type="application" data-filter="Immunocytochemistry/ Immunofluorescence">Immunocytochemistry/ Immunofluorescence</a> </div><div class="information-data">1 publication</div></li><li class="noIndent"><div class="information-subject"> <a href="#PublicationSection" class="filter_link" data-destination="publication" data-type="application" data-filter="Immunohistochemistry">Immunohistochemistry</a> </div><div class="information-data">2 publications</div></li><li class="noIndent"><div class="information-subject"> <a href="#PublicationSection" class="filter_link" data-destination="publication" data-type="application" data-filter="Simple Western">Simple Western</a> </div><div class="information-data">1 publication</div></li><li class="noIndent"><div class="information-subject"> <a href="#PublicationSection" class="filter_link" data-destination="publication" data-type="application" data-filter="WB">WB</a> </div><div class="information-data">3 publications</div></li><li class="noIndent"><div class="information-subject"> <a href="#PublicationSection" class="filter_link" data-destination="publication" data-type="application" data-filter="Western Blot">Western Blot</a> </div><div class="information-data">1 publication</div></li></ul></td> </tr> </table> </td> </tr> </table> </div><h2 class="greyHead2">Reactivity Notes</h2> <div> <a id="tab1speciesreactivity"></a> <div class="ds_info"> Porcine and mouse reactivity reported from verified customer reviews.</div> </div> <h2 class="greyHead2">Packaging, Storage & Formulations</h2><div class="ds_info"><table class="ds_list"><tr class="lined"> <td><strong>Storage</strong></td> <td><div>Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.</div></td> </tr><tr class="lined"> <td><strong>Buffer</strong></td> <td><div>PBS (pH 7.2), 40% Glycerol</div></td> </tr><tr class="lined"> <td><strong>Preservative</strong></td> <td><div>0.02% Sodium Azide</div></td> </tr><tr class="lined"> <td><strong>Purity</strong></td> <td><div>Immunogen affinity purified</div></td> </tr></table></div><h2 class="greyHead2">Alternate Names for Tight Junction Protein 1 Antibody</h2> <div class="ds_info"> <a id="tab1altnames"></a> <div class="information-list"> <ul><li>DKFZp686M05161</li><li>MGC133289</li><li>tight junction protein 1 (zona occludens 1)</li><li>Tight junction protein 1</li><li>tight junction protein ZO-1</li><li>TJP1</li><li>ZO1</li><li>ZO-1</li><li>zona occludens 1</li><li>Zona occludens protein 1</li><li>zonula occludens 1 protein</li><li>Zonula occludens protein 1</li></ul></div> </div> <h2 class="greyHead2">Background</h2> <div class="ds_info"> <a id="tab1background"></a> <div>Members of this family are involved in epithelial and endothelial intercellular junctions. They each contain at least one PSD95/Dlg/ZO-1 (PDZ) domain, a Src homology 3 (SH3) domain, and an enzymatically inactive guanylate kinase domain. PDZ domains are 90-amino acid protein-protein binding domains that recognize at least a 3-residue peptide motif in the COOH termini of their binding partners. PDZ domain-containing proteins, like ZO-1, typically act as scaffolding proteins that organize membrane receptors and cytosolic proteins into multimeric signaling complexes often at the sites of cell-cell contact. The effectiveness and stability of the epithelial barrier depends on a complex of proteins composing different intercellular junctions, which include tight junctions, adherens junctions, and desmosomes. ZO-1 is a peripheral membrane protein bound on the cytoplasmic surface of junctional contacts and is expressed in all tight junctions regardless of their properties. ZO-1 immunoprecipitates with its family member ZO-2. ZO-1 was shown to undergo tyrosine phosphorylation during tight junction formation and remodeling. Two different isoforms of ZO-1, alpha-minus and alpha-plus, have been described, which result from alternative splicing of an mRNA encoded by a single gene. The ZO-1 alpha-plus contains an 80 amino acids motif called alpha which is not present in ZO-1 alpha-minus. The alpha-containing isoform is found in most epithelial cell junctions. The short isoform (ZO-1 alpha-minus) is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules. This difference in distribution provides molecular distinction among tight junctions.</div> </div> <h2 class="greyHead2">Limitations</h2> <div class="ds_info"> <a id="tab1background"></a> <div>This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are <a href="/support/quality-assurance.html">guaranteed</a> for 1 year from date of receipt.</div> </div><h2 class="greyHead2">Customers Who Viewed This Item Also Viewed...</h2> <a id="custalsoviewed"></a><div id="cav_prev"><div class="sprite_leftArrow"></div></div> <div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout=0 data-cycle-prev="#cav_prev" data-cycle-next="#cav_next" data-cycle-slides="div.ds_cav_group" id="ds_customer_also_viewed"><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NBP1-87402"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/occludin-antibody_nbp1-87402" style="overflow: hidden; width: 280px;" title="Occludin Antibody">Occludin Antibody</a>    </div><div class="sub-header">NBP1-87402</div> <div class="images_customers"><div id="ss_prev_cav0" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-0" data-cycle-prev="#ss_prev_cav0" data-cycle-next="#ss_next_cav0" data-cycle-slides="div.big_thumb_cust_container" data-index=0 ><div class="big_thumb_cust_container"> <a href="/products/occludin-antibody_nbp1-87402" title="Western Blot: Occludin Antibody [NBP1-87402] - Lane 1: untreated vascular endothelial cells Lane 2: vascular endothelial cells + control siRNA. 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WB image submitted by a verified customer review." class="cust_viewed_0" > <img loading="lazy" src="https://images.novusbio.com/images/Occludin-Antibody-Western-Blot-NBP1-87402-img0009.jpg" alt="Western Blot Occludin Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/occludin-antibody_nbp1-87402" title="Immunohistochemistry-Paraffin: Occludin Antibody [NBP1-87402] - Staining of human breast cancer shows moderate membranous positivity in tumor cells." class="cust_viewed_0" > <img loading="lazy" src="https://images.novusbio.com/images/Occludin-Antibody-Immunohistochemistry-Paraffin-NBP1-87402-img0016.jpg" alt="Immunohistochemistry-Paraffin Occludin Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/occludin-antibody_nbp1-87402" title="Immunohistochemistry-Paraffin: Occludin Antibody [NBP1-87402] - Staining of human prostate cancer shows moderate membranous positivity in tumor cells." class="cust_viewed_0" > <img loading="lazy" src="https://images.novusbio.com/images/Occludin-Antibody-Immunohistochemistry-Paraffin-NBP1-87402-img0017.jpg" alt="Immunohistochemistry-Paraffin Occludin Antibody" class="big_thumb" /> </a> </div></div><div id="ss_next_cav0" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-0" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/occludin-antibody_nbp1-87402#ReviewsSection"> <div class="sprite_4Stars"></div> (4 Reviews</a>) </div> <div class="tiny"> <a href="/products/occludin-antibody_nbp1-87402#PublicationSection"> <div class="sprite_pubBook"></div> (23 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu, Mu, Rt</div><div class="tiny"><strong>Applications:</strong> ELISA, IHC, IHC-P, KD, WB</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NBP1-87402"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/occludin-antibody_nbp1-87402" style="overflow: hidden; width: 280px;" title="Occludin Antibody">Occludin Antibody</a>    </div><div class="sub-header">NBP1-87402</div> <div class="images_customers"><div id="ss_prev_cav1" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-1" data-cycle-prev="#ss_prev_cav1" data-cycle-next="#ss_next_cav1" data-cycle-slides="div.big_thumb_cust_container" data-index=1 ><div class="big_thumb_cust_container"> <a href="/products/occludin-antibody_nbp1-87402" title="Western Blot: Occludin Antibody [NBP1-87402] - Lane 1: untreated vascular endothelial cells Lane 2: vascular endothelial cells + control siRNA. 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WB image submitted by a verified customer review." class="cust_viewed_1" > <img loading="lazy" src="https://images.novusbio.com/images/Occludin-Antibody-Western-Blot-NBP1-87402-img0009.jpg" alt="Western Blot Occludin Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/occludin-antibody_nbp1-87402" title="Immunohistochemistry-Paraffin: Occludin Antibody [NBP1-87402] - Staining of human breast cancer shows moderate membranous positivity in tumor cells." class="cust_viewed_1" > <img loading="lazy" src="https://images.novusbio.com/images/Occludin-Antibody-Immunohistochemistry-Paraffin-NBP1-87402-img0016.jpg" alt="Immunohistochemistry-Paraffin Occludin Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/occludin-antibody_nbp1-87402" title="Immunohistochemistry-Paraffin: Occludin Antibody [NBP1-87402] - Staining of human prostate cancer shows moderate membranous positivity in tumor cells." class="cust_viewed_1" > <img loading="lazy" src="https://images.novusbio.com/images/Occludin-Antibody-Immunohistochemistry-Paraffin-NBP1-87402-img0017.jpg" alt="Immunohistochemistry-Paraffin Occludin Antibody" class="big_thumb" /> </a> </div></div><div id="ss_next_cav1" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-1" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/occludin-antibody_nbp1-87402#ReviewsSection"> <div class="sprite_4Stars"></div> (4 Reviews</a>) </div> <div class="tiny"> <a href="/products/occludin-antibody_nbp1-87402#PublicationSection"> <div class="sprite_pubBook"></div> (23 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu, Mu, Rt</div><div class="tiny"><strong>Applications:</strong> ELISA, IHC, IHC-P, KD, WB</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="H00009076-M01"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/claudin-1-antibody-1c5-d9_h00009076-m01" style="overflow: hidden; width: 280px;" title="Claudin-1 Antibody (1C5-D9)">Claudin-1 Antibody<br> (1C5-D9)</a>    </div><div class="sub-header">H00009076-M01</div> <div class="images_customers"><div id="ss_prev_cav2" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-2" data-cycle-prev="#ss_prev_cav2" data-cycle-next="#ss_next_cav2" data-cycle-slides="div.big_thumb_cust_container" data-index=2 ><div class="big_thumb_cust_container"> <a href="/products/claudin-1-antibody-1c5-d9_h00009076-m01" title="ELISA: Claudin-1 Antibody (1C5-D9) [H00009076-M01] - Detection limit for recombinant GST tagged CLDN1 is approximately 0.03ng/ml as a capture antibody." class="cust_viewed_2" > <img loading="lazy" src="https://images.novusbio.com/images/Claudin-1-Antibody-1C5-D9-ELISA-H00009076-M01-img0017.jpg" alt="ELISA Claudin-1 Antibody (1C5-D9)" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/claudin-1-antibody-1c5-d9_h00009076-m01" title="Immunohistochemistry-Paraffin: Claudin-1 Antibody (1C5-D9) [H00009076-M01] - Analysis of monoclonal antibody to CLDN1 on formalin-fixed paraffin-embedded human small Intestine. 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Antibody concentration 5 ug/ml." class="cust_viewed_2" > <img loading="lazy" src="https://images.novusbio.com/images/Claudin-1-Antibody-1C5-D9-Immunohistochemistry-Paraffin-H00009076-M01-img0011.jpg" alt="Immunohistochemistry-Paraffin Claudin-1 Antibody (1C5-D9)" class="big_thumb" /> </a> </div></div><div id="ss_next_cav2" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-2" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/claudin-1-antibody-1c5-d9_h00009076-m01#ReviewsSection"> <div class="sprite_5Stars"></div> (2 Reviews</a>) </div> <div class="tiny"> <a href="/products/claudin-1-antibody-1c5-d9_h00009076-m01#PublicationSection"> <div class="sprite_pubBook"></div> (26 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu, Pm, Mu</div><div class="tiny"><strong>Applications:</strong> ELISA, Flow, ICC/IF, IHC, IHC-P, IP, WB</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="AF748"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/e-cadherin-antibody_af748" style="overflow: hidden; width: 280px;" title="E-Cadherin Antibody [Unconjugated]">E-Cadherin<br> Antibody [Unconjugate</a>    </div><div class="sub-header">AF748</div> <div class="images_customers"><div id="ss_prev_cav3" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-3" data-cycle-prev="#ss_prev_cav3" data-cycle-next="#ss_next_cav3" data-cycle-slides="div.big_thumb_cust_container" data-index=3 ><div class="big_thumb_cust_container"> <a href="/products/e-cadherin-antibody_af748" title="E-Cadherin was detected in immersion fixed D3 mouse embryonic stem cell line using Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights&amp;#8482; 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips." class="cust_viewed_3" > <img loading="lazy" src="https://images.novusbio.com/images2/ECadherin_AF748_Immunocytochemistry_15885.jpg" alt="Immunocytochemistry E-Cadherin Antibody [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/e-cadherin-antibody_af748" title="E‑Cadherin was detected in perfusion fixed frozen sections of mouse skin using Goat Anti-Human/Mouse E‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748) at 1.7&amp;nbsp;µg/mL overnight at 4&amp;nbsp;°C. Tissue was stained using the NorthernLights&amp;#8482; 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog&amp;nbsp;# NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes in keratinocytes. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections. " class="cust_viewed_3" > <img loading="lazy" src="https://images.novusbio.com/images2/ECadherin_AF748_Immunohistochemistry_15886.jpg" alt="Immunohistochemistry E-Cadherin Antibody [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/e-cadherin-antibody_af748" title="E-Cadherin was detected in immersion fixed mouse intestinal organoids using Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights&amp;#8482; 557-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL001) and counterstained with DAPI (blue). Magnification shown at 100X (upper panel) and 40X (lower panel). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="cust_viewed_3" > <img loading="lazy" src="https://images.novusbio.com/images2/ECadherin_AF748_Immunocytochemistry_16572.jpg" alt="Immunocytochemistry E-Cadherin Antibody [Unconjugated]" class="big_thumb" /> </a> </div></div><div id="ss_next_cav3" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-3" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/e-cadherin-antibody_af748#PublicationSection"> <div class="sprite_pubBook"></div> (142 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu, Mu</div><div class="tiny"><strong>Applications:</strong> CyTOF-ready, Dual ISH-IHC, Flow, ICC, IHC, Simple Western, WB</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NBP2-02259"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/claudin-5-antibody-oti1g4_nbp2-02259" style="overflow: hidden; width: 280px;" title="Claudin-5 Antibody (OTI1G4)">Claudin-5 Antibody<br> (OTI1G4)</a>    </div><div class="sub-header">NBP2-02259</div> <div class="images_customers"><div id="ss_prev_cav4" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-4" data-cycle-prev="#ss_prev_cav4" data-cycle-next="#ss_next_cav4" data-cycle-slides="div.big_thumb_cust_container" data-index=4 ><div class="big_thumb_cust_container"> <a href="/products/claudin-5-antibody-oti1g4_nbp2-02259" title="Immunocytochemistry/Immunofluorescence: Claudin-5 Antibody (1G4) [NBP2-02259] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Claudin 5." class="cust_viewed_4" > <img loading="lazy" src="https://images.novusbio.com/images/Claudin-5-Antibody-1G4-Immunocytochemistry-Immunofluorescence-NBP2-02259-img0002.jpg" alt="Immunocytochemistry/ Immunofluorescence Claudin-5 Antibody (OTI1G4)" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/claudin-5-antibody-oti1g4_nbp2-02259" title="Immunocytochemistry/Immunofluorescence: Claudin-5 Antibody (OTI1G4) [NBP2-02259] - Claudin-5 labeled pig SC cells visualized by alexa 594. ICC/IF image submitted by a verified customer review." class="cust_viewed_4" > <img loading="lazy" src="https://images.novusbio.com/images/Claudin-5-Antibody-1G4-Immunocytochemistry-NBP2-02259-img0003.jpg" alt="Immunocytochemistry/ Immunofluorescence Claudin-5 Antibody (OTI1G4)" class="big_thumb" /> </a> </div></div><div id="ss_next_cav4" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-4" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/claudin-5-antibody-oti1g4_nbp2-02259#ReviewsSection"> <div class="sprite_5Stars"></div> (1 Review</a>) </div><div class="tiny"><strong>Species:</strong> Hu, Mu, Po, Rt</div><div class="tiny"><strong>Applications:</strong> ICC/IF</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="210-TA"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/recombinant-human-tnf-alpha-protein_210-ta" style="overflow: hidden; width: 280px;" title="TNF-alpha [Unconjugated]">TNF-alpha<br> [Unconjugated]</a>    </div><div class="sub-header">210-TA</div> <div class="images_customers"><div id="ss_prev_cav5" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-5" data-cycle-prev="#ss_prev_cav5" data-cycle-next="#ss_next_cav5" data-cycle-slides="div.big_thumb_cust_container" data-index=5 ><div class="big_thumb_cust_container"> <a href="/products/recombinant-human-tnf-alpha-protein_210-ta" title="1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa." class="cust_viewed_5" > <img loading="lazy" src="https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg" alt="SDS-PAGE TNF-alpha [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/recombinant-human-tnf-alpha-protein_210-ta" title="Recombinant Human TNF-alpha (Catalog # 210‑TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 25‑100 pg/mL." class="cust_viewed_5" > <img loading="lazy" src="https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg" alt="Bioactivity TNF-alpha [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/recombinant-human-tnf-alpha-protein_210-ta" title="Recombinant Human TNF-alpha (Catalog # 210-TA) has a molecular weight (MW) of 53.1 kDa as analyzed by SEC-MALS, suggesting that this protein is a homotrimer. MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation)." class="cust_viewed_5" > <img loading="lazy" src="https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg" alt="SEC-MALS TNF-alpha [Unconjugated]" class="big_thumb" /> </a> </div></div><div id="ss_next_cav5" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-5" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/recombinant-human-tnf-alpha-protein_210-ta#ReviewsSection"> <div class="sprite_5Stars"></div> (3 Reviews</a>) </div> <div class="tiny"> <a href="/products/recombinant-human-tnf-alpha-protein_210-ta#PublicationSection"> <div class="sprite_pubBook"></div> (811 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu</div><div class="tiny"><strong>Applications:</strong> BA</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NB300-556"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/psd-95-antibody-6g6-1c9_nb300-556" style="overflow: hidden; width: 280px;" title="PSD-95 Antibody (6G6-1C9) - BSA Free">PSD-95 Antibody<br> (6G6-1C9) - BSA Free</a>    </div><div class="sub-header">NB300-556</div> <div class="images_customers"><div id="ss_prev_cav6" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-6" data-cycle-prev="#ss_prev_cav6" data-cycle-next="#ss_next_cav6" data-cycle-slides="div.big_thumb_cust_container" data-index=6 ><div class="big_thumb_cust_container"> <a href="/products/psd-95-antibody-6g6-1c9_nb300-556" title="Flow Cytometry: PSD-95 Antibody (6G6-1C9) [NB300-556] - An intracellular stain was performed on HeLa cells with PSD-95 Antibody (6G6-1C9) NB300-556AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488." class="cust_viewed_6" > <img loading="lazy" src="https://images.novusbio.com/images/PSD-95-Antibody-6G6-1C9-Flow-Cytometry-NB300-556-img0023.jpg" alt="Flow Cytometry PSD-95 Antibody (6G6-1C9) - BSA Free" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/psd-95-antibody-6g6-1c9_nb300-556" title="Immunocytochemistry/Immunofluorescence: PSD-95 Antibody (6G6-1C9) [NB300-556] - Mixed 3 week old rat cortical cultures (21 DIV) were labelled with anti-PSD95 (1:650) and visualised with anti-mouse Alexa647 (1:2000). The antibody labels small punctae along neuronal processes as expected. This image was submitted via customer Review." class="cust_viewed_6" > <img loading="lazy" src="https://images.novusbio.com/images/PSD-95-Antibody-6G6-1C9-Immunocytochemistry-Immunofluorescence-NB300-556-img0021.jpg" alt="Immunocytochemistry/ Immunofluorescence PSD-95 Antibody (6G6-1C9) - BSA Free" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/psd-95-antibody-6g6-1c9_nb300-556" title="Immunocytochemistry/Immunofluorescence: PSD-95 Antibody (6G6-1C9) [NB300-556] - Control immunolocalization of plasma membrane AMPA receptor subunits (external epitopes) in hippocampal neuronal cultures. H) Immunolabeling of non-permeabilized neuronal cultures with ani-GluA1 (green). (I) Immunolabeling with the postsynaptic marker anti-PSD95 [66] after permeabilization of neuronal cultures. (J) Merging of (H) and (I) showing postsynaptic labeling of GluA1. Scale bar (valid for all images): 10 um. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0140868), licensed under a CC-BY license." class="cust_viewed_6" > <img loading="lazy" src="https://images.novusbio.com/images/PSD-95-Antibody-6G6-1C9-Immunocytochemistry-Immunofluorescence-NB300-556-img0025.jpg" alt="Immunocytochemistry/ Immunofluorescence PSD-95 Antibody (6G6-1C9) - BSA Free" class="big_thumb" /> </a> </div></div><div id="ss_next_cav6" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-6" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/psd-95-antibody-6g6-1c9_nb300-556#ReviewsSection"> <div class="sprite_5Stars"></div> (1 Review</a>) </div> <div class="tiny"> <a href="/products/psd-95-antibody-6g6-1c9_nb300-556#PublicationSection"> <div class="sprite_pubBook"></div> (31 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu, In, Mu, Pm, Rt</div><div class="tiny"><strong>Applications:</strong> B/N, ChIP, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="AF1329"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/beta-catenin-antibody_af1329" style="overflow: hidden; width: 280px;" title="beta-Catenin Antibody [Unconjugated]">beta-Catenin<br> Antibody [Unconjugate</a>    </div><div class="sub-header">AF1329</div> <div class="images_customers"><div id="ss_prev_cav7" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-7" data-cycle-prev="#ss_prev_cav7" data-cycle-next="#ss_next_cav7" data-cycle-slides="div.big_thumb_cust_container" data-index=7 ><div class="big_thumb_cust_container"> <a href="/products/beta-catenin-antibody_af1329" title="Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, C6 rat glioma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse/Rat beta -Catenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1329) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human beta -catenin (1 ng) was included. A specific band for beta-Catenin was detected at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="cust_viewed_7" > <img loading="lazy" src="https://images.novusbio.com/images2/beta-Catenin_AF1329_Western_Blot_5100.jpg" alt="Western Blot beta-Catenin Antibody [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/beta-catenin-antibody_af1329" title="HeLa human cervical epithelial carcinoma cell line were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. beta ‑Catenin/DNA complexes were immuno&amp;shy;precipitated using 5 μg Goat Anti-Human/Mouse/Rat beta ‑Catenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1329) or control antibody (Catalog&amp;nbsp;# AB‑108‑C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog&amp;nbsp;# BAF109). Immuno&amp;shy;complexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog&amp;nbsp;# MAG999) and DNA was purified using chelating resin solution. The SU(Z)12 promoter was detected by standard PCR." class="cust_viewed_7" > <img loading="lazy" src="https://images.novusbio.com/images2/beta-Catenin_AF1329_Chromatin_Immunoprecipitation_11251.jpg" alt="Chromatin Immunoprecipitation (ChIP) beta-Catenin Antibody [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/beta-catenin-antibody_af1329" title="Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and NIH‑3T3 mouse embryonic fibroblast cell line, loaded at 0.2&amp;nbsp;mg/mL. A specific band was detected for beta ‑Catenin at approximately 94-97&amp;nbsp;kDa (as indicated) using 50&amp;nbsp;µg/mL of Goat Anti-Human/Mouse/Rat beta ‑Catenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1329) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog&amp;nbsp;# HAF109). This experiment was conducted under reducing&amp;nbsp;conditions and using the12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody." class="cust_viewed_7" > <img loading="lazy" src="https://images.novusbio.com/images2/betaCatenin_AF1329_Simple_Western_17134.jpg" alt="Simple Western beta-Catenin Antibody [Unconjugated]" class="big_thumb" /> </a> </div></div><div id="ss_next_cav7" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-7" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/beta-catenin-antibody_af1329#ReviewsSection"> <div class="sprite_4Stars"></div> (2 Reviews</a>) </div> <div class="tiny"> <a href="/products/beta-catenin-antibody_af1329#PublicationSection"> <div class="sprite_pubBook"></div> (33 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu, Mu, Rt</div><div class="tiny"><strong>Applications:</strong> ChIP, CyTOF-ready, ICC, IHC, ICFlow, Simple Western, WB</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="MAB4219"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/claudin-4-antibody-382321_mab4219" style="overflow: hidden; width: 280px;" title="Claudin-4 Antibody (382321) [Unconjugated]">Claudin-4 Antibody<br> (382321) [Unconjugate ...</a>    </div><div class="sub-header">MAB4219</div> <div class="images_customers"><div class="one_image_l"></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-8" data-cycle-prev="#ss_prev_cav8" data-cycle-next="#ss_next_cav8" data-cycle-slides="div.big_thumb_cust_container" data-index=8 ><div class="big_thumb_cust_container"> <a href="/products/claudin-4-antibody-382321_mab4219" title="PC-3 human prostate cancer cell line was stained with Mouse Anti-Human Claudin-4 Monoclonal Antibody (Catalog # MAB4219, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram) followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins." class="cust_viewed_8" > <img loading="lazy" src="https://images.novusbio.com/images2/Claudin4_MAB4219_Flow_Cytometry_21352.jpg" alt="Flow Cytometry Claudin-4 Antibody (382321) [Unconjugated]" class="big_thumb" /> </a> </div></div><div class="one_image"></div><div class="one_image_11"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/claudin-4-antibody-382321_mab4219#PublicationSection"> <div class="sprite_pubBook"></div> (2 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu</div><div class="tiny"><strong>Applications:</strong> CyTOF-ready, Flow</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="DVE00"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/human-vegf-quantikine-elisa-kit_dve00" style="overflow: hidden; width: 280px;" title="VEGF [HRP]">VEGF [HRP]</a>    </div><div class="sub-header">DVE00</div> <div class="images_customers"><div id="ss_prev_cav9" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-9" data-cycle-prev="#ss_prev_cav9" data-cycle-next="#ss_next_cav9" data-cycle-slides="div.big_thumb_cust_container" data-index=9 ><div class="big_thumb_cust_container"> <a href="/products/human-vegf-quantikine-elisa-kit_dve00" title="" class="cust_viewed_9" > <img loading="lazy" src="https://images.novusbio.com/images2/DATA_VEGF_DVE00_ELISA_871.jpg" alt="N/A VEGF [HRP]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/human-vegf-quantikine-elisa-kit_dve00" title="" class="cust_viewed_9" > <img loading="lazy" src="https://images.novusbio.com/images2/DATA_VEGF_DVE00_ELISA_872.jpg" alt="N/A VEGF [HRP]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/human-vegf-quantikine-elisa-kit_dve00" title="" class="cust_viewed_9" > <img loading="lazy" src="https://images.novusbio.com/images2/VEGF_DVE00_ELISA_208.jpg" alt="N/A VEGF [HRP]" class="big_thumb" /> </a> </div></div><div id="ss_next_cav9" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-9" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/human-vegf-quantikine-elisa-kit_dve00#PublicationSection"> <div class="sprite_pubBook"></div> (682 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu</div><div class="tiny"><strong>Applications:</strong> ELISA</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NBP1-86850"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/tight-junction-protein-2-antibody_nbp1-86850" style="overflow: hidden; width: 280px;" title="Tight Junction Protein 2 Antibody">Tight Junction<br> Protein 2 Antibody</a>    </div><div class="sub-header">NBP1-86850</div> <div class="images_customers"><div id="ss_prev_cav10" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-10" data-cycle-prev="#ss_prev_cav10" data-cycle-next="#ss_next_cav10" data-cycle-slides="div.big_thumb_cust_container" data-index=10 ><div class="big_thumb_cust_container"> <a href="/products/tight-junction-protein-2-antibody_nbp1-86850" title="Immunocytochemistry/Immunofluorescence: Tight Junction Protein 2 Antibody [NBP1-86850] - Staining of human cell line U-2 OS shows positivity in plasma membrane, cytoplasm and cell junctions. Antibody staining is shown in green." class="cust_viewed_10" > <img loading="lazy" src="https://images.novusbio.com/images/Tight-Junction-Protein-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86850-img0014.jpg" alt="Immunocytochemistry/ Immunofluorescence Tight Junction Protein 2 Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/tight-junction-protein-2-antibody_nbp1-86850" title="Immunocytochemistry/Immunofluorescence: Tight Junction Protein 2 Antibody [NBP1-86850] - Staining in primary mouse lung endothelial cells. Image submitted by a verified customer review." class="cust_viewed_10" > <img loading="lazy" src="https://images.novusbio.com/images/Tight-Junction-Protein-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86850-img0017.jpg" alt="Immunocytochemistry/ Immunofluorescence Tight Junction Protein 2 Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/tight-junction-protein-2-antibody_nbp1-86850" title="Immunohistochemistry-Paraffin: Tight Junction Protein 2 Antibody [NBP1-86850] - Staining of human testis shows strong membranous and cytoplasmic positivity in cells in seminiferous ducts." class="cust_viewed_10" > <img loading="lazy" src="https://images.novusbio.com/images/Tight-Junction-Protein-2-Antibody-Immunohistochemistry-Paraffin-NBP1-86850-img0019.jpg" alt="Immunohistochemistry-Paraffin Tight Junction Protein 2 Antibody" class="big_thumb" /> </a> </div></div><div id="ss_next_cav10" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-10" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/tight-junction-protein-2-antibody_nbp1-86850#ReviewsSection"> <div class="sprite_5Stars"></div> (5 Reviews</a>) </div> <div class="tiny"> <a href="/products/tight-junction-protein-2-antibody_nbp1-86850#PublicationSection"> <div class="sprite_pubBook"></div> (4 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu, Mu</div><div class="tiny"><strong>Applications:</strong> ICC/IF, IHC, IHC-P, IP, WB</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NBP3-18555"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/claudin-3-antibody-cl13130_nbp3-18555" style="overflow: hidden; width: 280px;" title="Claudin-3 Antibody (CL13130)">Claudin-3 Antibody<br> (CL13130)</a>    </div><div class="sub-header">NBP3-18555</div> <div class="images_customers"><div id="ss_prev_cav11" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-11" data-cycle-prev="#ss_prev_cav11" data-cycle-next="#ss_next_cav11" data-cycle-slides="div.big_thumb_cust_container" data-index=11 ><div class="big_thumb_cust_container"> <a href="/products/claudin-3-antibody-cl13130_nbp3-18555" title="Immunocytochemistry/Immunofluorescence: Claudin-3 Antibody (CL13130) [NBP3-18555] - Staining of MCF-7 cells using the Anti-CLDN3 monoclonal antibody, showing specific staining in cell junctions in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available)." class="cust_viewed_11" > <img loading="lazy" src="https://images.novusbio.com/images/Claudin-3-Antibody-CL13130-Immunocytochemistry-Immunofluorescence-NBP3-18555-img0001.jpg" alt="Immunocytochemistry/ Immunofluorescence Claudin-3 Antibody (CL13130)" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/claudin-3-antibody-cl13130_nbp3-18555" title="Immunohistochemistry-Paraffin: Claudin-3 Antibody (CL13130) [NBP3-18555] - Analysis in human prostate and placenta tissues using NBP3-18555 antibody. Corresponding CLDN3 RNA-seq data are presented for the same tissues." class="cust_viewed_11" > <img loading="lazy" src="https://images.novusbio.com/images/Claudin-3-Antibody-CL13130-Immunohistochemistry-Paraffin-NBP3-18555-img0002.jpg" alt="Immunohistochemistry-Paraffin Claudin-3 Antibody (CL13130)" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/claudin-3-antibody-cl13130_nbp3-18555" title="Immunohistochemistry-Paraffin: Claudin-3 Antibody (CL13130) [NBP3-18555] - Staining of human ovarian cancer (clear cell carcinoma) shows strong membranous positivity in tumor cells." class="cust_viewed_11" > <img loading="lazy" src="https://images.novusbio.com/images/Claudin-3-Antibody-CL13130-Immunohistochemistry-Paraffin-NBP3-18555-img0003.jpg" alt="Immunohistochemistry-Paraffin Claudin-3 Antibody (CL13130)" class="big_thumb" /> </a> </div></div><div id="ss_next_cav11" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-11" class="cycle-circle-pager"></div></div> <div class="ds_info"><div class="tiny"><strong>Species:</strong> Hu</div><div class="tiny"><strong>Applications:</strong> ICC/IF, IHC, IHC-P, WB</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NBP2-49230"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/synaptic-glycoprotein-sc2-antibody_nbp2-49230" style="overflow: hidden; width: 280px;" title="Synaptic glycoprotein SC2 Antibody">Synaptic<br> glycoprotein SC2 Anti</a>    </div><div class="sub-header">NBP2-49230</div> <div class="images_customers"><div id="ss_prev_cav12" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-12" data-cycle-prev="#ss_prev_cav12" data-cycle-next="#ss_next_cav12" data-cycle-slides="div.big_thumb_cust_container" data-index=12 ><div class="big_thumb_cust_container"> <a href="/products/synaptic-glycoprotein-sc2-antibody_nbp2-49230" title="Western Blot: Synaptic glycoprotein SC2 Antibody [NBP2-49230] - Analysis in control (vector only transfected HEK293T lysate) and TECR over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells)." class="cust_viewed_12" > <img loading="lazy" src="https://images.novusbio.com/images/Synaptic-glycoprotein-SC2-Antibody-Western-Blot-NBP2-49230-img0005.jpg" alt="Western Blot Synaptic glycoprotein SC2 Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/synaptic-glycoprotein-sc2-antibody_nbp2-49230" title="Immunocytochemistry/Immunofluorescence: Synaptic glycoprotein SC2 Antibody [NBP2-49230] - Immunofluorescent staining of human cell line U-251 MG shows localization to endoplasmic reticulum." class="cust_viewed_12" > <img loading="lazy" src="https://images.novusbio.com/images/Synaptic-glycoprotein-SC2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-49230-img0004.jpg" alt="Immunocytochemistry/ Immunofluorescence Synaptic glycoprotein SC2 Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/synaptic-glycoprotein-sc2-antibody_nbp2-49230" title="Immunohistochemistry-Paraffin: Synaptic glycoprotein SC2 Antibody [NBP2-49230] - Staining of human rectum shows moderate cytoplasmic positivity in glandular cells." class="cust_viewed_12" > <img loading="lazy" src="https://images.novusbio.com/images/Synaptic-glycoprotein-SC2-Antibody-Immunohistochemistry-Paraffin-NBP2-49230-img0002.jpg" alt="Immunohistochemistry-Paraffin Synaptic glycoprotein SC2 Antibody" class="big_thumb" /> </a> </div></div><div id="ss_next_cav12" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-12" class="cycle-circle-pager"></div></div> <div class="ds_info"><div class="tiny"><strong>Species:</strong> Hu</div><div class="tiny"><strong>Applications:</strong> ICC/IF, IHC, IHC-P, WB</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NBP2-38696"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/tjp3-antibody_nbp2-38696" style="overflow: hidden; width: 280px;" title="TJP3 Antibody">TJP3 Antibody</a>    </div><div class="sub-header">NBP2-38696</div> <div class="images_customers"><div id="ss_prev_cav13" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-13" data-cycle-prev="#ss_prev_cav13" data-cycle-next="#ss_next_cav13" data-cycle-slides="div.big_thumb_cust_container" data-index=13 ><div class="big_thumb_cust_container"> <a href="/products/tjp3-antibody_nbp2-38696" title="Immunohistochemistry-Paraffin: TJP3 Antibody [NBP2-38696] - Staining of human fallopian tube shows moderate cell membrane positivity in glandular cells." class="cust_viewed_13" > <img loading="lazy" src="https://images.novusbio.com/images/TJP3-Antibody-Immunohistochemistry-Paraffin-NBP2-38696-img0003.jpg" alt="Immunohistochemistry-Paraffin TJP3 Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/tjp3-antibody_nbp2-38696" title="Immunohistochemistry-Paraffin: TJP3 Antibody [NBP2-38696] - Staining of human prostate shows low expression as expected." class="cust_viewed_13" > <img loading="lazy" src="https://images.novusbio.com/images/TJP3-Antibody-Immunohistochemistry-Paraffin-NBP2-38696-img0004.jpg" alt="Immunohistochemistry-Paraffin TJP3 Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/tjp3-antibody_nbp2-38696" title="Immunohistochemistry-Paraffin: TJP3 Antibody [NBP2-38696] - Staining in human fallopian tube and prostate tissues using anti-TJP3 antibody. Corresponding TJP3 RNA-seq data are presented for the same tissues." class="cust_viewed_13" > <img loading="lazy" src="https://images.novusbio.com/images/TJP3-Antibody-Immunohistochemistry-Paraffin-NBP2-38696-img0005.jpg" alt="Immunohistochemistry-Paraffin TJP3 Antibody" class="big_thumb" /> </a> </div></div><div id="ss_next_cav13" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-13" class="cycle-circle-pager"></div></div> <div class="ds_info"><div class="tiny"><strong>Species:</strong> Hu</div><div class="tiny"><strong>Applications:</strong> ICC/IF, IHC, IHC-P</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NBP2-00594"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/guanylate-kinase-antibody-oti4a8_nbp2-00594" style="overflow: hidden; width: 280px;" title="Guanylate kinase Antibody (OTI4A8)">Guanylate kinase<br> Antibody (OTI4A8)</a>    </div><div class="sub-header">NBP2-00594</div> <div class="images_customers"><div id="ss_prev_cav14" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-14" data-cycle-prev="#ss_prev_cav14" data-cycle-next="#ss_next_cav14" data-cycle-slides="div.big_thumb_cust_container" data-index=14 ><div class="big_thumb_cust_container"> <a href="/products/guanylate-kinase-antibody-oti4a8_nbp2-00594" title="Flow Cytometry: Guanylate kinase Antibody (4A8) [NBP2-00594] - Analysis of Hela cells, using anti-Guanylate kinase antibody, (Red), compared to a nonspecific negative control antibody (Blue)." class="cust_viewed_14" > <img loading="lazy" src="https://images.novusbio.com/images/Guanylate-kinase-Antibody-4A8-Flow-Cytometry-NBP2-00594-img0001.jpg" alt="Flow Cytometry Guanylate kinase Antibody (OTI4A8)" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/guanylate-kinase-antibody-oti4a8_nbp2-00594" title="Flow Cytometry: Guanylate kinase Antibody (4A8) [NBP2-00594] - Analysis of Jurkat cells, using anti-Guanylate kinase antibody, (Red), compared to a nonspecific negative control antibody (Blue)." class="cust_viewed_14" > <img loading="lazy" src="https://images.novusbio.com/images/Guanylate-kinase-Antibody-4A8-Flow-Cytometry-NBP2-00594-img0002.jpg" alt="Flow Cytometry Guanylate kinase Antibody (OTI4A8)" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/guanylate-kinase-antibody-oti4a8_nbp2-00594" title="Flow Cytometry: Guanylate kinase Antibody (4A8) [NBP2-00594] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Guanylate kinase antibody, and then analyzed by flow cytometry." class="cust_viewed_14" > <img loading="lazy" src="https://images.novusbio.com/images/Guanylate-kinase-Antibody-4A8-Flow-Cytometry-NBP2-00594-img0003.jpg" alt="Flow Cytometry Guanylate kinase Antibody (OTI4A8)" class="big_thumb" /> </a> </div></div><div id="ss_next_cav14" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-14" class="cycle-circle-pager"></div></div> <div class="ds_info"><div class="tiny"><strong>Species:</strong> Hu, Mu, Rt</div><div class="tiny"><strong>Applications:</strong> Flow, IHC, IHC-P, WB</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="AF1230"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/erk2-antibody_af1230" style="overflow: hidden; width: 280px;" title="ERK2 Antibody [Unconjugated]">ERK2 Antibody<br> [Unconjugated]</a>    </div><div class="sub-header">AF1230</div> <div class="images_customers"><div id="ss_prev_cav15" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-15" data-cycle-prev="#ss_prev_cav15" data-cycle-next="#ss_next_cav15" data-cycle-slides="div.big_thumb_cust_container" data-index=15 ><div class="big_thumb_cust_container"> <a href="/products/erk2-antibody_af1230" title="Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, U937 human histiocytic lymphoma cell line, PC-12 rat adrenal pheochromocytoma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse/Rat ERK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1230) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for ERK2 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="cust_viewed_15" > <img loading="lazy" src="https://images.novusbio.com/images2/ERK2_AF1230_Western_Blot_5097.jpg" alt="Western Blot ERK2 Antibody [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/erk2-antibody_af1230" title=" ERK2 was detected in immersion fixed paraffin-embedded sections of human breast using Rabbit Anti-Human/Mouse/Rat ERK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1230) at 3&amp;nbsp;µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte&amp;#8482; HRP Polymer Antibody (Catalog&amp;nbsp;# VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents. " class="cust_viewed_15" > <img loading="lazy" src="https://images.novusbio.com/images2/ERK2_AF1230_Immunohistochemistry_20696.jpg" alt="Immunohistochemistry ERK2 Antibody [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/erk2-antibody_af1230" title="Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and ERK2 knockout HeLa cell line (KO). PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse/Rat ERK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1230) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for ERK2 at approximately 42 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="cust_viewed_15" > <img loading="lazy" src="https://images.novusbio.com/images2/ERK2_AF1230_Knockout_Validated_22864.jpg" alt="Knockout Validated ERK2 Antibody [Unconjugated]" class="big_thumb" /> </a> </div></div><div id="ss_next_cav15" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-15" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/erk2-antibody_af1230#PublicationSection"> <div class="sprite_pubBook"></div> (3 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu, Mu, Rt</div><div class="tiny"><strong>Applications:</strong> IHC, KO, WB</div> </div> </div> </div></div> </div><div id="cav_next"><div class="sprite_rightArrow"></div></div> <div class="clear"></div></div> <div class="ds_tab_content equalbottom" id="content_reviews-publications"> <a id="reviews-publications"></a><a id="PublicationSection"></a><h2 class="greyHead2">Publications for Tight Junction Protein 1 Antibody (NBP1-85047)(20)</h2><div class="ds_info"> <div>We have publications tested in 3 confirmed species: Human, Mouse, Rat.<br /><br />We have publications tested in 9 applications: Block/Neutralize, ICC/IF, IF/IHC, IHC-P, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Simple Western, WB, Western Blot.<br /><br /><div class="align-right"> <button class="greybutton btn_width" name="novusreview" onclick="window.location='/publications'"; >Submit a Publication</button><br /> </div> </div><div> <div class="filterParent"> <div class="publications_filterList" id="pubapp"> <div class="blueHead">Filter By Application</div><div class="filter_line"> <input type="checkbox" value="application" class="publication_filterCheckbox" data-filter="Block/Neutralize"> <div style="display:inline;">Block/Neutralize</div> (1)</div><div class="filter_line"> <input type="checkbox" value="application" class="publication_filterCheckbox" data-filter="ICC/IF"> <div style="display:inline;">ICC/IF</div> (4)</div><div class="filter_line"> <input type="checkbox" value="application" class="publication_filterCheckbox" data-filter="IF/IHC"> <div style="display:inline;">IF/IHC</div> (1)</div><div class="filter_line"> <input type="checkbox" value="application" class="publication_filterCheckbox" data-filter="IHC-P"> <div style="display:inline;">IHC-P</div> (2)</div><div class="filter_line"> <input type="checkbox" value="application" class="publication_filterCheckbox" data-filter="Immunocytochemistry/ Immunofluorescence"> <div style="display:inline;">Immunocytochemistry/ Immunofluorescence</div> (1)</div><div class="filter_line"> <input type="checkbox" value="application" class="publication_filterCheckbox" data-filter="Immunohistochemistry"> <div style="display:inline;">Immunohistochemistry</div> (2)</div><div class="filter_line"> <input type="checkbox" value="application" class="publication_filterCheckbox" data-filter="Simple Western"> <div style="display:inline;">Simple Western</div> (1)</div><div class="filter_line"> <input type="checkbox" value="application" class="publication_filterCheckbox" data-filter="WB"> <div style="display:inline;">WB</div> (3)</div><div class="filter_line"> <input type="checkbox" value="application" class="publication_filterCheckbox" data-filter="Western Blot"> <div style="display:inline;">Western Blot</div> (1)</div><div class="filter_clear" data-type="publication" data-filter="application">All Applications</div></div> <div class="publications_filterList" id="pubspec"> <div class="blueHead">Filter By Species</div><div class="filter_line"> <input type="checkbox" value="species" class="publication_filterCheckbox" data-filter="Human"> <div style="display:inline;">Human</div> (3)</div><div class="filter_line"> <input type="checkbox" value="species" class="publication_filterCheckbox" data-filter="Mouse"> <div style="display:inline;">Mouse</div> (2)</div><div class="filter_line"> <input type="checkbox" value="species" class="publication_filterCheckbox" data-filter="Rat"> <div style="display:inline;">Rat</div> (1)</div><div class="filter_clear" data-type="publication" data-filter="species">All Species</div></div> </div></div> <div class="clear"></div> <div id="publications_list">Showing Publications 1 - <span id="appcnt">10</span> of 20. <a class="pubs_showall">Show All 20 Publications.</a> <a class="pubs_collapse" style="display:none;">Collapse Publications.</a><table class="wide lightPad tablesorter" id="publications_list_table"> <thead> <tr> <th>Publications using NBP1-85047</th> <th>Applications</th> <th>Species</th> </tr> </thead> <tbody><tr class="lined revfil firstten" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/38560893" target="_blank" rel="nofollow"> Rion J Wendland, Budd A Tucker, Kristan S Worthington Influence of Substrate Stiffness on iPSC-Derived Retinal Pigmented Epithelial Cells Stem Cells Translational Medicine 2024-06-01 [PMID: 38560893] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil firstten" data-application="IHC-P" data-species="Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/37056910" target="_blank" rel="nofollow"> Cheng Y, Li J, Zhang X et al. Protective Effect of Qingchang Wenzhong Decoction on Colitis and Colitis-Related Carcinogenesis by Regulating Inflammation and Intestinal Fibrosis Journal of inflammation research 2023-04-07 [PMID: 37056910] (IHC-P, Mouse) </a></td> <td valign="top">IHC-P</td> <td valign="top">Mouse</td> </tr><tr class="lined revfil firstten" data-application="" data-species=""> <td> </td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil firstten" data-application="Western Blot" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/36091013" target="_blank" rel="nofollow"> Ji S, You Y, Peng B et al. Multi-omics analysis reveals the metabolic regulators of duodenal low-grade inflammation in a functional dyspepsia model Frontiers in Immunology 2022-08-24 [PMID: 36091013] (Western Blot) </a></td> <td valign="top">Western Blot</td> <td valign="top"></td> </tr><tr class="lined revfil firstten" data-application="Block/Neutralize" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/36056566" target="_blank" rel="nofollow"> Rosu GC, Mateescu VO, Simionescu A et al. Subtle vascular and astrocytic changes in the brain of coronavirus disease 2019 (COVID-19) patients European Journal of Neurology 2022-12-01 [PMID: 36056566] (Block/Neutralize) </a></td> <td valign="top">Block/Neutralize</td> <td valign="top"></td> </tr><tr class="lined revfil firstten" data-application="Immunohistochemistry" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/35814685" target="_blank" rel="nofollow"> Yang AM, Lin CY, Liu SH et al. Saccharomyces Boulardii Ameliorates Non-alcoholic Steatohepatitis in Mice Induced by a Methionine-Choline-Deficient Diet Through Gut-Liver Axis Frontiers in Microbiology 2022-06-23 [PMID: 35814685] (Immunohistochemistry) </a></td> <td valign="top">Immunohistochemistry</td> <td valign="top"></td> </tr><tr class="lined revfil firstten" data-application="WB, ICC/IF" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/35440001" target="_blank" rel="nofollow"> Fu Q, Lin Q, Chen D et al. beta-defensin 118 attenuates inflammation and injury of intestinal epithelial cells upon enterotoxigenic Escherichia coli challenge BMC veterinary research 2022-04-19 [PMID: 35440001] (WB, ICC/IF) </a></td> <td valign="top">WB, ICC/IF</td> <td valign="top"></td> </tr><tr class="lined revfil firstten" data-application="WB" data-species="Rat"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/35101948" target="_blank" rel="nofollow"> Song K, Zeng X, Xie X et al. Dl-3-n-butylphthalide attenuates brain injury caused by cortical infarction accompanied by cranial venous drainage disturbance Stroke and vascular neurology 2022-01-31 [PMID: 35101948] (WB, Rat) </a></td> <td valign="top">WB</td> <td valign="top">Rat</td> </tr><tr class="lined revfil firstten" data-application="Immunohistochemistry" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/34539414" target="_blank" rel="nofollow"> Spindler LM, Feuerhake A, Ladel S et al. Nano-in-Micro-Particles Consisting of PLGA Nanoparticles Embedded in Chitosan Microparticles via Spray-Drying Enhances Their Uptake in the Olfactory Mucosa Frontiers in Pharmacology 2021-09-01 [PMID: 34539414] (Immunohistochemistry) </a></td> <td valign="top">Immunohistochemistry</td> <td valign="top"></td> </tr><tr class="lined revfil firstten" data-application="IHC-P" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/34305900" target="_blank" rel="nofollow"> Lassiter R, Merchen Td, Fang X, Wang Y Protective Role of Kynurenine 3-Monooxygenase in Allograft Rejection and Tubular Injury in Kidney Transplantation Frontiers in immunology 2021-07-07 [PMID: 34305900] (IHC-P) </a></td> <td valign="top">IHC-P</td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/34234159" target="_blank" rel="nofollow"> Manon Boul, Nassima Benzoubir, Antonietta Messina, Rasta Ghasemi, Ismail Ben Mosbah, Jean-Charles Duclos-Vallée, Anne Dubart-Kupperschmitt, Bruno Le Pioufle A versatile microfluidic tool for the 3D culture of HepaRG cells seeded at various stages of differentiation Scientific Reports 2021-07-07 [PMID: 34234159] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="Simple Western" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/33941937" target="_blank" rel="nofollow"> Chen Y, Tristan CA, Lu C et al. A Versatile Polypharmacology Platform Promotes Cytoprotection and Viability of Human Pluripotent and Differentiated Cells Nat Methods 2021-05-04 [PMID: 33941937] (Simple Western) </a></td> <td valign="top">Simple Western</td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="ICC/IF" data-species="Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/33664246" target="_blank" rel="nofollow"> Abbassi L, El-Hayek S, Carvalho KF et al. Epidermal growth factor receptor signaling uncouples germ cells from the somatic follicular compartment at ovulation Nature communications 2021-03-04 [PMID: 33664246] (ICC/IF, Mouse) </a></td> <td valign="top">ICC/IF</td> <td valign="top">Mouse</td> </tr><tr class="lined revfil hidden" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/32846954" target="_blank" rel="nofollow"> Hahn L, Helmrich N, Herebian D et al. IL-13 as Target to Reduce Cholestasis and Dysbiosis in Abcb4 Knockout Mice Cells 2020-08-24 [PMID: 32846954] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="Immunocytochemistry/ Immunofluorescence" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/32468024" target="_blank" rel="nofollow"> Su C, Liu S, Ma X et al. Decitabine attenuates dextran sodium sulfate?induced ulcerative colitis through regulation of immune regulatory cells and intestinal barrier International Journal of Molecular Medicine 2020-05-18 [PMID: 32468024] (Immunocytochemistry/ Immunofluorescence) </a></td> <td valign="top">Immunocytochemistry/ Immunofluorescence</td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="WB" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/32391146" target="_blank" rel="nofollow"> Liu X, Zhang B, Liu H et al. Determination of the available energy values and amino acid digestibility of Flammulina velutipes stem waste and its effects on carcass trait and meat quality fed to growing-finishing pigs J Anim Sci Biotechnol 2020-05-04 [PMID: 32391146] (WB) </a></td> <td valign="top">WB</td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="ICC/IF" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/31358055" target="_blank" rel="nofollow"> Caron Jerome, Pene Veronique, Tolosa Laia et al. Low-density lipoprotein receptor-deficient hepatocytes differentiated from induced pluripotent stem cells allow familial hypercholesterolemia modeling, CRISPR/Cas-mediated genetic correction, and productive hepatitis C virus infection. Stem Cell Research & Therapy 2019-07-29 [PMID: 31358055] (ICC/IF) </a></td> <td valign="top">ICC/IF</td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="ICC/IF" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/30173956" target="_blank" rel="nofollow"> Coffin KM, Liu J, Warren TK et al. Persistent Marburg Virus Infection in the Testes of Nonhuman Primate Survivors. Cell Host Microbe 2018-09-12 [PMID: 30173956] (ICC/IF, Human) </a></td> <td valign="top">ICC/IF</td> <td valign="top">Human</td> </tr><tr class="lined revfil hidden" data-application="" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/29615456" target="_blank" rel="nofollow"> Liu M, Yang J, Zhang Y et al. ZIP4 Promotes Pancreatic Cancer Progression by Repressing ZO-1 and claudin-1 through a ZEB1-Dependent Transcriptional Mechanism. Clin. Cancer Res. 2018-04-03 [PMID: 29615456] (Human) </a></td> <td valign="top"></td> <td valign="top">Human</td> </tr><tr class="lined revfil hidden" data-application="IF/IHC" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/24300555" target="_blank" rel="nofollow"> McIntyre BA, Alev C, Mechael R et al. Expansive Generation of Functional Airway Epithelium From Human Embryonic Stem Cells. Stem Cells Transl Med 2014-01-01 [PMID: 24300555] (IF/IHC, Human) </a></td> <td valign="top">IF/IHC</td> <td valign="top">Human</td> </tr><tr><td colspan="3" align="center"> <a class="pubs_showall">Show All 20 Publications.</a> <a class="pubs_collapse" style="display:none;">Collapse Publications.</a> </td></tr></tbody> </table> </div> </div><a id="ReviewsSection"></a> <h2 class="greyHead2">Reviews for <span>Tight Junction Protein 1 Antibody (NBP1-85047)</span> (5) <span style="display:none;">4.6</span><span style="display:none;">5</span></h2> <div class="ds_info"> <div class="bottom15"> <strong>Average Rating: 4.6</strong> <div class="dsSprite_stars5"></div> (Based on 5 reviews) </div> <div><div class="ds_info">We have 5 reviews tested in 4 species: Human, Mouse, Rat, Other.</div> <div class="align-right"> <button class="greybutton btn_width" id="novusreview" name="novusreview" onclick="window.location='/reviews/NBP1-85047'"; >Submit a Review</button><br /> </div> </div> <div class="blueHead"><strong>Reviews using NBP1-85047:</strong></div> <div class="filterParent"><div class="reviews_filterList"> <div class="blueHead">Filter by Applications</div><div class="filter_line"> <input type="checkbox" value="application" class="review_filterCheckbox" data-filter="ICC"> <div style="display:inline;">ICC</div> (3)</div><div class="filter_line"> <input type="checkbox" value="application" class="review_filterCheckbox" data-filter="WB"> <div style="display:inline;">WB</div> (1)</div><div class="filter_line"> <input type="checkbox" value="application" class="review_filterCheckbox" data-filter="ICC/IF"> <div style="display:inline;">ICC/IF</div> (1)</div><div class="filter_clear" data-type="review" data-filter="application">All Applications</div></div> <div class="reviews_filterList"> <div class="blueHead">Filter by Species</div><div class="filter_line"> <input type="checkbox" value="species" class="review_filterCheckbox" data-filter="Human"> <div style="display:inline;">Human</div> (1)</div><div class="filter_line"> <input type="checkbox" value="species" class="review_filterCheckbox" data-filter="Mouse"> <div style="display:inline;">Mouse</div> (2)</div><div class="filter_line"> <input type="checkbox" value="species" class="review_filterCheckbox" data-filter="Rat"> <div style="display:inline;">Rat</div> (1)</div><div class="filter_line"> <input type="checkbox" value="species" class="review_filterCheckbox" data-filter="Other"> <div style="display:inline;">Other</div> (1)</div><div class="filter_clear" data-type="review" data-filter="species">All Species</div></div></div><div id="reviews_list"> <table class="wide lightPad tablesorter" id="reviews_list_table"> <thead> <tr> <th>Images</th> <th>Ratings</th> <th>Applications</th> <th>Species</th> <th>Date</th> <th>Details</th> </tr> </thead> <tbody><tr class="top-lined revfil" data-rating="Excellent" data-application="ICC" data-species="Rat"> <td><a href="https://www.novusbio.com/sites/novusbio.com/files/reviews/review_nbp1-85047_54701_0_0.jpg" title="Rat epithelial cells stained positively with Tight Junction Protein 1. Primary ab concentration = 1:100; Secondary ab: goat anti-rabbit- Alexa 488 conjugated = 1: 500" class="colorbox" title="Immunocytochemistry" > <img loading="lazy" src="https://www.novusbio.com/sites/novusbio.com/files/reviews/review_nbp1-85047_54701_0_0.jpg" class="reviews_imageBorder" width="75" alt="Immunocytochemistry Tight Junction Protein 1 NBP1-85047" /> <br/><span class="enlarge" href="#"><div class="dsSprite_magGlass"></div> Enlarge</span></a><meta content="12/10/2020" /> </td> <td> <span style="display:none;">5</span> <div class="dsSprite_stars5" content="5"></div> <div class="revby">reviewed by:<br />James Xiao</div> </td> <td title="Immunocytochemistry">ICC</td> <td>Rat</td> <td>12/10/2020</td> <td align="left"> <div class="dsSprite_expandReviews"><div class="expandReviewData">View</div></div> <div class="reviews_showMoreContent"> <div class="reviews_showMoreContentInner"><h2 class="greyHead">Summary</h2> <table class="even wide top"><tr><td class="text_bold">Application</td><td>Immunocytochemistry</td></tr><tr><td class="text_bold">Sample Tested</td><td>Epithelial cells</td></tr><tr><td class="text_bold">Species</td><td>Rat</td></tr><tr><td class="text_bold">Lot</td><td>000001836</td></tr></table> </div> </div> </td> </tr><tr class="top-lined revfil" data-rating="Excellent" data-application="ICC" data-species="Human"> <td><a href="https://www.novusbio.com/sites/novusbio.com/files/reviews/review_nbp1-85047_35771.jpg" title="ARPE-19 monolayer on glass substrate" class="colorbox" title="Immunocytochemistry" > <img loading="lazy" src="https://www.novusbio.com/sites/novusbio.com/files/reviews/review_nbp1-85047_35771.jpg" class="reviews_imageBorder" width="75" alt="Immunocytochemistry Tight Junction Protein 1 NBP1-85047" /> <br/><span class="enlarge" href="#"><div class="dsSprite_magGlassBlue"></div> Enlarge</span></a><meta content="08/29/2017" /> </td> <td> <span style="display:none;">5</span> <div class="dsSprite_stars5" content="5"></div> <div class="revby">reviewed by:<br />Fabio Marongiu</div> </td> <td title="Immunocytochemistry">ICC</td> <td>Human</td> <td>08/29/2017</td> <td align="left"> <div class="dsSprite_expandReviews"><div class="expandReviewData">View</div></div> <div class="reviews_showMoreContent"> <div class="reviews_showMoreContentInner"><h2 class="greyHead">Summary</h2> <table class="even wide top"><tr><td class="text_bold">Application</td><td>Immunocytochemistry</td></tr><tr><td class="text_bold">Sample Tested</td><td>ARPE-19 cells</td></tr><tr><td class="text_bold">Species</td><td>Human</td></tr><tr><td class="text_bold">Lot</td><td>C105864</td></tr></table><h2 class="greyHead">Comments</h2> <table class="even wide top"><tr><td class="text_bold">Comments</td><td>Fixative 4% PFA<br />Blocking Goat Serum<br />Dilution 1:200<br />Incubation 1h @ RT</td></tr></table> </div> </div> </td> </tr><tr class="top-lined revfil" data-rating="Very Good" data-application="WB" data-species="Mouse"> <td><a href="https://www.novusbio.com/sites/novusbio.com/files/reviews/review_nbp1-85047_33096.png" title="Lane 1: untreated vascular endothelial cellsLane 2: vascular endothelial cells + control siRNA" class="colorbox" title="Western Blot" > <img loading="lazy" src="https://www.novusbio.com/sites/novusbio.com/files/reviews/review_nbp1-85047_33096.png" class="reviews_imageBorder" width="75" alt="Western Blot Tight Junction Protein 1 NBP1-85047" /> <br/><span class="enlarge" href="#"><div class="dsSprite_magGlass"></div> Enlarge</span></a><meta content="06/22/2017" /> </td> <td> <span style="display:none;">4</span> <div class="dsSprite_stars4" content="4"></div> <div class="revby">reviewed by:<br />James Xiao</div> </td> <td title="Western Blot">WB</td> <td>Mouse</td> <td>06/22/2017</td> <td align="left"> <div class="dsSprite_expandReviews"><div class="expandReviewData">View</div></div> <div class="reviews_showMoreContent"> <div class="reviews_showMoreContentInner"><h2 class="greyHead">Summary</h2> <table class="even wide top"><tr><td class="text_bold">Application</td><td>Western Blot</td></tr><tr><td class="text_bold">Sample Tested</td><td>Primary Mouse Lung cells</td></tr><tr><td class="text_bold">Species</td><td>Mouse</td></tr></table> </div> </div> </td> </tr><tr class="top-lined revfil" data-rating="Excellent" data-application="ICC/IF" data-species="Mouse"> <td><a href="https://www.novusbio.com/sites/novusbio.com/files/reviews/review_nbp1-85047_25631_0.jpg" title="The primary mouse lung endothelial cells were fixed, permeabilized and stained with 1:100 diluted anti-ZO-1 ab for overnight. Samples were washed and subsequently incubated with 1:500 diluted Alexa Fuor 546 at room temperature for 1 hour. " class="colorbox" title="Other" > <img loading="lazy" src="https://www.novusbio.com/sites/novusbio.com/files/reviews/review_nbp1-85047_25631_0.jpg" class="reviews_imageBorder" width="75" alt="Other Tight Junction Protein 1 NBP1-85047" /> <br/><span class="enlarge" href="#"><div class="dsSprite_magGlassBlue"></div> Enlarge</span></a><meta content="10/13/2016" /> </td> <td> <span style="display:none;">5</span> <div class="dsSprite_stars5" content="5"></div> <div class="revby">reviewed by:<br />James Xiao</div> </td> <td title="Other">ICC/IF</td> <td>Mouse</td> <td>10/13/2016</td> <td align="left"> <div class="dsSprite_expandReviews"><div class="expandReviewData">View</div></div> <div class="reviews_showMoreContent"> <div class="reviews_showMoreContentInner"><h2 class="greyHead">Summary</h2> <table class="even wide top"><tr><td class="text_bold">Application</td><td>Other</td></tr><tr><td class="text_bold">Sample Tested</td><td>Primary mouse lung endotelial cells</td></tr><tr><td class="text_bold">Species</td><td>Mouse</td></tr><tr><td class="text_bold">Lot</td><td>B10562</td></tr></table> </div> </div> </td> </tr><tr class="top-lined revfil" data-rating="Very Good" data-application="ICC" data-species="Other"> <td><a href="https://images.novusbio.com/reviews/Immunocytochemistry__NBP1-85047_23081.jpg" title="Detection of TJP1 in Pig trabecular meshwork cells" class="colorbox" title="Immunocytochemistry" > <img loading="lazy" src="https://images.novusbio.com/reviews/Immunocytochemistry__NBP1-85047_23081.jpg" class="reviews_imageBorder" width="75" alt="Immunocytochemistry Tight Junction Protein 1 NBP1-85047" /> <br/><span class="enlarge" href="#"><div class="dsSprite_magGlass"></div> Enlarge</span></a><meta content="02/02/2016" /> </td> <td> <span style="display:none;">4</span> <div class="dsSprite_stars4" content="4"></div> <div class="revby">reviewed by:<br />Verified Customer</div> </td> <td title="Immunocytochemistry">ICC</td> <td>Other</td> <td>02/02/2016</td> <td align="left"> <div class="dsSprite_expandReviews"><div class="expandReviewData">View</div></div> <div class="reviews_showMoreContent"> <div class="reviews_showMoreContentInner"><h2 class="greyHead">Summary</h2> <table class="even wide top"><tr><td class="text_bold">Application</td><td>Immunocytochemistry</td></tr><tr><td class="text_bold">Sample Tested</td><td>Pig trabecular meshwork cells</td></tr><tr><td class="text_bold">Species</td><td>Other</td></tr></table> </div> </div> </td> </tr></tbody> </table> </div><br /> </div> <div class="clear"></div></div> <div class="ds_tab_content equalbottom" id="content_protocols-faqs"> <a id="protocols-faqs"></a><h2 class="greyHead2">Product General Protocols</h2> <a id="protocols"></a> <div class="ds_info">Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars. <ul><li><a href="/support/support-by-application/immunocytochemistry/protocol.html">Immunocytochemistry/ Immunofluorescence</a></li><li><a href="/support/support-by-application/immunohistochemistry-paraffin/protocol.html">Immunohistochemistry-Paraffin</a></li><li><a href="/support/support-by-application/western-blot/protocol.html">Western Blot</a></li><li><a href="/support/support-by-application.html">View all Protocols, Troubleshooting, Illustrated assays and Webinars.</a></li> </ul></div><h2 class="greyHead2 ds_info">Video Protocols</h2> <a id="videos"></a> <div class="ds_info"><div class="movie-cell"> <span class="movie-title">WB Video Protocol</span><br/> <meta content ="https://www.youtube.com/watch?v=uFu8aie4QFI" /> <a href="https://www.youtube.com/embed/uFu8aie4QFI?rel=0&wmode=transparent" class="youtube"> <div class="reduce sprite_WBvideo"></div> </a> </div><div class="movie-cell"> <span class="movie-title">ICC/IF Video Protocol</span><br/> <meta content ="https://www.youtube.com/watch?v=5AcrhhHmQZs" /> <a href="https://www.youtube.com/embed/5AcrhhHmQZs?rel=0&wmode=transparent" class="youtube"> <div class="reduce sprite_ICC-IFvideo"></div> </a> </div></div> <div class="clear"></div><h2 class="greyHead2">FAQs for Tight Junction Protein 1 Antibody (NBP1-85047) (0)</h2> <a id="faqs"></a> <div class="ds_info">There are no specific FAQs related to this product. Read our general <a href="/support/faqs.html">customer & technical service FAQs.</a> </div> <div class="clear"></div></div> <div class="ds_tab_content equalbottom" id="content_supportresearch"> <a id="supportresearch"></a><table cellpadding="0" cellspacing="0"><tr><td valign="top" class="width50"> <h2 class="greyHead2">Secondary Antibodies</h2> <a id="secondaryantibodies"></a> <div class="ds_info"> <ul class="information-list"><li><a href="/product-type/secondary-antibodies?species=Rabbit&facet_conjugate=HRP">Anti-Rabbit HRP Labeled Antibodies</a></li><li><a href="/product-type/secondary-antibodies?species=Rabbit&facet_conjugate=FITC">Anti-Rabbit FITC Labeled Antibodies</a></li><li><a href="/product-type/secondary-antibodies?species=Rabbit&facet_conjugate=Biotin">Anti-Rabbit Biotin Labeled Antibodies</a></li><li> </li> <li><a href="/product-type/secondary-antibodies#fq=species%3A%22Rabbit%22">View all of our anti-Rabbit Secondary Antibodies</a></li> </ul> </div></td><td width="5"> </td><td valign="top"> <h2 class="greyHead2" >Isotype Controls</h2> <a id="isotypecontrols"></a> <div class="ds_info"> <ul class="ds_noDotList"><li><a href="/product-type/isotype-controls#fq=species%3A%22Rabbit%22">Rabbit Isotype Controls</a></li><li> </li> <li><a href="/product-type/isotype-controls">View all of our Isotype Control Products</a></li> </div></td></tr></table><h2 class="greyHead2">Additional Tight Junction Protein 1 Products</h2> <a id="addtlalphaprods"></a> <div class="ds_info"> <div> <span style="display:none;">Array NBP1-85047</span> <ul class="ds_noDotList"><li> <a href="/primary-antibodies/tight-junction-protein-1">Tight Junction Protein 1 Antibodies</a></li><li> <a href="/elisa-kits/tight-junction-protein-1">Tight Junction Protein 1 ELISA Kits</a></li><li> <a href="/lysates/tight-junction-protein-1">Tight Junction Protein 1 Lysate</a></li><li> <a href="/proteins/tight-junction-protein-1">Tight Junction Protein 1 Proteins</a></li></ul> </div> </div><table cellpadding="0" cellspacing="0" class="width100"><tr></tr></table><table cellpadding="0" cellspacing="0" class="width100"><tr></tr></table><h2 class="greyHead2">Blogs on Tight Junction Protein 1. </h2> <a id="blogsect"></a> <div class="ds_info"><a href="/antibody-news/tag/tight-junction-protein-1">Check out the latest blog posts on Tight Junction Protein 1</a>.</div> <div class="ds_info"> <table id="blogs_list_table"> <tbody> <tr class=" blogwrap "> <td> <strong><a href="/antibody-news/antibodies/zonula-occludens-zo-the-junction-scaffolding-proteins">Zonula Occludens (ZO) the Junction Scaffolding Proteins</a></strong><br/> <span>The Zonula Occludens (ZO) proteins 1,2 and 3, also known as tight junction proteins, are peripheral proteins localizing at junctional sites(1). ZO proteins are known to be scaffolding proteins recruiting various types of proteins to the cytoplasmic...  <a href="/antibody-news/antibodies/zonula-occludens-zo-the-junction-scaffolding-proteins">Read full blog post.</a></span><br /><br /> </td> </tr></tbody> </table> </div> <div class="clear"></div></div> </div></div><div class="grid_6"><div class="ds_blue_border marTop26 equalbottom"><div class="datasheet-ads-container" ><a href="https://www.bio-techne.com/resources/blogs/citation-widget-find-products-used-in-literature" target="_blank"><img loading="lazy" src="https://images.novusbio.com/design/citation-app-banner-349x119-v3.jpg" alt="Read our latest blog and use the new citation tool on bio-techne.com" title="new citation tool" class="datasheet-adzone" loading="lazy"></a></div><a id="jump_recommended"></a> <h2 class="greyHead2">Customers Who Bought This Also Bought</h2> <div class="text_center"><div class="images2"><div id="ss_prev_sidebar" class="cycle-prev ds_rp_prev"><div class="pager_left_on"></div></div><div id="ss_next_sidebar" class="cycle-next ds_rp_next"><div class="pager_right_on"></div></div><div class="cycle-slideshow" data-cycle-fx="scrollHorz" data-cycle-timeout="0" data-cycle-prev="#ss_prev_sidebar" data-cycle-next="#ss_next_sidebar" data-cycle-slides="div.big_thumb_container" id="ds_customer_who_bought" ><div class="big_thumb_container" data-catnums="HAF008"> <div class="img_container"> <a href="/products/igg-antibody_haf008" title="Western blot shows detection of Rabbit Anti-Human/Mouse/Rat HSP60 Polyclonal Antibody (AF1800) using a 1:1000 dilution of HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="cust_bought" > <img loading="lazy" src="https://images.novusbio.com/images2/IgG_HAF008_Western_Blot_19080.jpg" title="Western blot shows detection of Rabbit Anti-Human/Mouse/Rat HSP60 Polyclonal Antibody (AF1800) using a 1:1000 dilution of HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows detection of Rabbit Anti-Human/Mouse/Rat HSP60 Polyclonal Antibody (AF1800) using a 1:1000 dilution of HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" /> </a> </div> <div class="caption_container"><a href="/products/igg-antibody_haf008">Goat anti-Rabbit IgG Secondary Antibody [HRP]</a> <br />HAF008</div> <div style="text-align: right"><button class="greybutton" id="novusviewdatasheet" name="novusviewdatasheet" onclick="window.location='/products/igg-antibody_haf008';" >View Datasheet</button> </div> </div><div class="big_thumb_container" data-catnums="NB7160"> <div class="img_container"> <a href="/products/igg-h-l-antibody_nb7160" title="Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). 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Goat anti-rabbit FITC (Novus, 20302) was used as secondary." class="cust_bought" > <img loading="lazy" src="https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg" title="Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary." alt="Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary." class="big_thumb" /> </a> </div> <div class="caption_container"><a href="/products/igg-isotype-control_nbp2-24891">Rabbit IgG Isotype Control</a> <br />NBP2-24891</div> <div style="text-align: right"><button class="greybutton" id="novusviewdatasheet" name="novusviewdatasheet" onclick="window.location='/products/igg-isotype-control_nbp2-24891';" >View Datasheet</button> </div> </div></div></div></div><div class="clear"></div><a id="jump_distributor"></a> <h2 class="greyHead2">Contact Information</h2> <div class="ds_info"> <div id="distributors"></div> </div> <div id="et_reload_trigger" style="display:none;">reload</div><h2 class="greyHead2">Product PDFs</h2><div class="silverHeadShort"> <span class="file"><a href="//aero.bio-techne.com/sds/pdf/nbp1-85047" target="_blank" class="sprite_dsPDF"><span class="pdfSmaller"> </span></a></span> <span class="download-file-text" ><a href="//aero.bio-techne.com/sds/pdf/nbp1-85047" target="_blank" class="datasheet-download sdsFile">SDS</a></span> </div><div class="silverHeadShort"> <span class="file"><a href="//www.novusbio.com/PDFs/NBP1-85047.pdf" target="_blank" class="sprite_dsPDF"><span class="pdfSmaller"> </span></a></span> <span class="download-file-text" ><a href="//www.novusbio.com/PDFs/NBP1-85047.pdf" target="_blank" class="datasheet-download">PDF Datasheet</a></span> </div><div class="silverHeadShort"> <span class="file"><a href="mailto:novus@novusbio.com?subject=Request%20CofA%20for%20NBP1-85047" class="sprite_newDocument"><span class="downloadBlank"> </span></a></span> <span class="download-file-text"><a href="mailto:nb-technical@bio-techne.com?subject=Request%20CofA%20for%20NBP1-85047&body=Specific%20catalog%20number(if multiple sizes):%0ALot%20Number:%0AAny%20additional%20products:">Request CofA</a></span> </div><h2 class="greyHead2">Calculators</h2> <div class="calc_wrapper"> <div class="dsSprite_calc"></div> <div class="fake_link" id="calc_reconstitution_link">Concentration Calculator</div> <div id="calc_reconstitution_wrapper" class="calc_function_wrapper"> <div class="calc_popup"> <div class="calc_close"> <div class="dsSprite_calc_close"></div> </div> <div class="calc_header">Concentration Calculator</div> <div id="reconstitution_calculator" class="calculator"> <div class="reconstitution_calculator_text" id="reconstitution_calculator_instructions"> <p>The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. 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Primary ab concentration = 1:100; Secondary ab: goat anti-rabbit- Alexa 488 conjugated = 1: 500" class="colorbox" title="Immunocytochemistry" > <img loading="lazy" src="//www.novusbio.com/sites/novusbio.com/files/reviews/review_nbp1-85047_54701_0_0.jpg" class="reviews_imageBorder" width="75" alt="Immunocytochemistry Tight Junction Protein 1 NBP1-85047" /> <br/><span class="enlarge" href="#"><div class="dsSprite_magGlass"></div> Enlarge</span></a></div> <div class="review_summary_right"><table> <tbody> <tr> <td><div class="dsSprite_stars5" content="5"></div></td> <td>James Xiao<br />12/10/2020</td> </tr> <tr> <td class="review_summary_title">Application: </td> <td>ICC<td> </tr> <tr> <td class="review_summary_title">Species: </td> <td>Rat</td> </tr> </tbody> </table> <div class="text_right" style="margin-bottom:-8px;"><a href="#ReviewsSection">read this review</a></div></div></div> <hr /><div class="single_review"><div class="review_summary_image"><a 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