PARD3/Par3 Antibody (NBP1-88861): Novus Biologicals

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5.1724696046-473375462.1712005244" style="margin: 0 10px;" target="_blank"><img alt="Novus Biologicals products are now on bio-techne.com" src="https://images.novusbio.com/design/ready-to-buy-novus-products-login.png" style="height:120px; width:940px" /></a></div> </div> </div> </div> <h1>PARD3/Par3 Antibody</h1> </div> <div class="clear"></div> <div> <div class="region region-content"> <div id="block-et-messages-et-messages-block" class="block block-et-messages"> <div class="content"> <span></span> </div> </div> <div id="block-system-main" class="block block-system"> <div class="content"> <div class="grid_16"><div class="ds_info float_left"><a href="#PublicationSection"><div class="sprite_pubBook_large"></div></a> <a href="#PublicationSection" class="bottom2"><span class="ctr">(10 Publications)</span></a></div><div class="ds_info float_left"><a href="#ReviewsSection"><div class="dsSprite_stars4"></div></a> <a href="#ReviewsSection" class="bottom0"><span class="ctr">(2 Reviews)</span></a></div><div class="ds_info float_left"><a href="/proteinsimple" title="Learn more about Simple Western"><div class="sprite_swBadge simple"> </div></a></div><div class="ds_info float_left"> <a href="/common-name/pard3-par3"><div class="sprite_allRelated"></div></a> <a href="/common-name/pard3-par3" class="bottom4"><span class="ctr">See All Related</span></a> </div><div class="ds_info float_left"><a href="/reviews/NBP1-88861"><div class="dsSprite_submitreview"></div></a> <a href="/reviews/NBP1-88861" class="bottom4"><span class="ctr">Submit a Review</span></a></div></div></div><div class="modalWrapper" style="display:none;"> <h2>Species Glossary</h2> <hr class="customhr"/> <img src="//images.novusbio.com/design/bunny.gif" alt="Species Glossary Rabbit" style="float: right;"> <table class="modalTable"> <tr> <td>At</td> <td>=</td> <td>A. thaliana</td> <td>SyHa</td> <td>=</td> <td>Golden Syrian Hamster</td> </tr> <tr> <td>All</td> <td>=</td> <td>All Species</td> <td>Ha</td> <td>=</td> <td>Hamster</td> </tr> <tr> <td>An</td> <td>=</td> <td>Animal</td> <td>Hu</td> <td>=</td> <td>Human</td> </tr> <tr> <td>ArHa</td> <td>=</td> <td>Armenian Hamster</td> <td>I</td> <td>=</td> <td>Insect</td> </tr> <tr> <td>Av</td> <td>=</td> <td>Avian</td> <td>Ll</td> <td>=</td> <td>Llama</td> </tr> <tr> <td>Bb</td> <td>=</td> <td>Baboon</td> <td>Ma</td> <td>=</td> <td>Mammal</td> </tr> <tr> <td>Ba</td> <td>=</td> <td>Bacteria</td> <td>Mk</td> <td>=</td> <td>Monkey</td> </tr> <tr> <td>Bv</td> <td>=</td> <td>Bovine</td> <td>Mu</td> <td>=</td> <td>Mouse</td> </tr> <tr> <td>Ca</td> <td>=</td> <td>Canine</td> <td>Multi</td> <td>=</td> <td>Multi-species</td> </tr> <tr> <td>Ce</td> <td>=</td> <td>C. Elegans</td> <td>NA</td> <td>=</td> <td>Non-species specific</td> </tr> <tr> <td>Ch</td> <td>=</td> <td>Chicken</td> <td>Or</td> <td>=</td> <td>Orangutan</td> </tr> <tr> <td>ChHa</td> <td>=</td> <td>Chinese Hamster</td> <td>Pl</td> <td>=</td> <td>Plant</td> </tr> <tr> <td>Cm</td> <td>=</td> <td>Cynomolgus Monkey</td> <td>Pm</td> <td>=</td> <td>Primate</td> </tr> <tr> <td>Do</td> <td>=</td> <td>Donkey</td> <td>Po</td> <td>=</td> <td>Porcine</td> </tr> <tr> <td>Dr</td> <td>=</td> <td>Drosophilia</td> <td>Rb</td> <td>=</td> <td>Rabbit</td> </tr> <tr> <td>Ec</td> <td>=</td> <td>E. Coli</td> <td>Rt</td> <td>=</td> <td>Rat</td> </tr> <tr> <td>Eq</td> <td>=</td> <td>Equine</td> <td>RM</td> <td>=</td> <td>Rhesus Macaque</td> </tr> <tr> <td>Fe</td> <td>=</td> <td>Feline</td> <td>Sh</td> <td>=</td> <td>Sheep</td> </tr> <tr> <td>Ft</td> <td>=</td> <td>Ferret</td> <td>Vb</td> <td>=</td> <td>Vertebrate</td> </tr> <tr> <td>Fi</td> <td>=</td> <td>Fish</td> <td>Vi</td> <td>=</td> <td>Virus</td> </tr> <tr> <td>Fu</td> <td>=</td> <td>Fungi</td> <td>Xp</td> <td>=</td> <td>Xenopus</td> </tr> <tr> <td>Ge</td> <td>=</td> <td>Gerbil</td> <td>Ye</td> <td>=</td> <td>Yeast</td> </tr> <tr> <td>GP</td> <td>=</td> <td>Guinea Pig</td> <td>Ze</td> <td>=</td> <td>Zebrafish</td> </tr> <tr> <td>Gt</td> <td>=</td> <td>Goat</td> </tr> </table> <div style="text-align: center;margin-left:25px;margin-right:25px;"> <p>Note: Mouseover a species abbreviation on the product page to display the fullname.</p> </div> </div> </div><div class="clear"></div><div class="grid_10 pos_relative"><div class="ds_blue_border equaltop"><div class="imagesleft equaltop"><h2 class="blueHead">Images</h2> <div class="midBlue"> </div><div class="images"><div id="ss_prev_top" class="cycle-pager"><div class="pager_left_on"></div></div><div class="cycle-slideshow slideshows" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-top" data-cycle-prev="#ss_prev_top" data-cycle-next="#ss_next_top" data-cycle-caption="#image-caption" data-cycle-caption-template="Image {{slideNum}} of {{slideCount}}" data-cycle-slides=".big_thumb_container" data-cycle-carousel-visible="5" id="images_cycle" > <div class="sub_image_wrapper"><div id="cycle-circle-pager-top" class="cycle-circle-pager"></div><div id="image-caption"></div> <a class="enlargeTop">(<div class="dsSprite_magGlass"></div> Enlarge)</a></div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize/PARD3-Par3-Antibody-Western-Blot-NBP1-88861-img0017.jpg" class="top_images" rel="gallery" title="<b>Orthogonal Strategies Validation. </b>Western Blot: PARD3/Par3 Antibody [NBP1-88861] - Analysis in human cell lines A-549 and U-251MG. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH." > <img src="//images.novusbio.com/images/PARD3-Par3-Antibody-Western-Blot-NBP1-88861-img0017.jpg" class="big_thumb" alt="Western Blot: PARD3/Par3 Antibody [NBP1-88861] - Analysis in human cell lines A-549 and U-251MG. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH." title="Western Blot: PARD3/Par3 Antibody [NBP1-88861] - Analysis in human cell lines A-549 and U-251MG. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH." /> </a> </div> <div class="alt-caption padding_left5" ><b>Orthogonal Strategies</b>: Western Blot: PARD3/Par3 Antibody [NBP1-88861] - Analysis in human cell lines A-549 and U-251MG. Corresponding RNA-seq data are presented for the same cell lines. Loading control:<a class="openGallery" href="#" data-picnum="0"> ...read more</a></div> </div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize/PARD3-Par3-Antibody-Simple-Western-NBP1-88861-img0009.jpg" class="top_images" rel="gallery" title="Simple Western: PARD3/Par3 Antibody [NBP1-88861] - Simple Western lane view shows a specific band for PARD3/Par3 in 0.2 mg/ml of RT-4 lysate. This experiment was performed under reducing conditions using the 66-440 kDa separation system." > <img src="//images.novusbio.com/images/PARD3-Par3-Antibody-Simple-Western-NBP1-88861-img0009.jpg" loading="lazy" style="display:none;" class="big_thumb" alt="Simple Western: PARD3/Par3 Antibody [NBP1-88861] - Simple Western lane view shows a specific band for PARD3/Par3 in 0.2 mg/ml of RT-4 lysate. This experiment was performed under reducing conditions using the 66-440 kDa separation system." title="Simple Western: PARD3/Par3 Antibody [NBP1-88861] - Simple Western lane view shows a specific band for PARD3/Par3 in 0.2 mg/ml of RT-4 lysate. This experiment was performed under reducing conditions using the 66-440 kDa separation system." /> </a> </div> <div class="alt-caption padding_left5" >Simple Western: PARD3/Par3 Antibody [NBP1-88861] - Simple Western lane view shows a specific band for PARD3/Par3 in 0.2 mg/ml of RT-4 lysate. This experiment was performed under reducing conditions using the 66-440 kDa<a class="openGallery" href="#" data-picnum="1"> ...read more</a></div> </div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize/PARD3-Par3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88861-img0016.jpg" class="top_images" rel="gallery" title="Immunocytochemistry/Immunofluorescence: PARD3/Par3 Antibody [NBP1-88861] - Staining of human cell line U-2 OS shows localization to cell junctions. Antibody staining is shown in green." > <img src="//images.novusbio.com/images/PARD3-Par3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88861-img0016.jpg" loading="lazy" style="display:none;" class="big_thumb" alt="Immunocytochemistry/Immunofluorescence: PARD3/Par3 Antibody [NBP1-88861] - Staining of human cell line U-2 OS shows localization to cell junctions. Antibody staining is shown in green." title="Immunocytochemistry/Immunofluorescence: PARD3/Par3 Antibody [NBP1-88861] - Staining of human cell line U-2 OS shows localization to cell junctions. Antibody staining is shown in green." /> </a> </div> <div class="alt-caption padding_left5" >Immunocytochemistry/ Immunofluorescence: PARD3/Par3 Antibody [NBP1-88861] - Staining of human cell line U-2 OS shows localization to cell junctions. Antibody staining is shown in green.</div> </div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize/PARD3-Par3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88861-img0012.jpg" class="top_images" rel="gallery" title="Immunocytochemistry/Immunofluorescence: PARD3/Par3 Antibody [NBP1-88861] - Staining PARD3 in mouse trophoblast stem cells. Verified customer review from 1DegreeBio." > <img src="//images.novusbio.com/images/PARD3-Par3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88861-img0012.jpg" loading="lazy" style="display:none;" class="big_thumb" alt="Immunocytochemistry/Immunofluorescence: PARD3/Par3 Antibody [NBP1-88861] - Staining PARD3 in mouse trophoblast stem cells. Verified customer review from 1DegreeBio." title="Immunocytochemistry/Immunofluorescence: PARD3/Par3 Antibody [NBP1-88861] - Staining PARD3 in mouse trophoblast stem cells. Verified customer review from 1DegreeBio." /> </a> </div> <div class="alt-caption padding_left5" >Immunocytochemistry/ Immunofluorescence: PARD3/Par3 Antibody [NBP1-88861] - Staining PARD3 in mouse trophoblast stem cells. Verified customer review from 1DegreeBio.</div> </div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize/PARD3-Par3-Antibody-Immunohistochemistry-Paraffin-NBP1-88861-img0014.jpg" class="top_images" rel="gallery" title="Immunohistochemistry-Paraffin: PARD3/Par3 Antibody [NBP1-88861] - Staining of human colon shows distinct luminal membranous positivity in glandular cells." > <img src="//images.novusbio.com/images/PARD3-Par3-Antibody-Immunohistochemistry-Paraffin-NBP1-88861-img0014.jpg" loading="lazy" style="display:none;" class="big_thumb" alt="Immunohistochemistry-Paraffin: PARD3/Par3 Antibody [NBP1-88861] - Staining of human colon shows distinct luminal membranous positivity in glandular cells." title="Immunohistochemistry-Paraffin: PARD3/Par3 Antibody [NBP1-88861] - Staining of human colon shows distinct luminal membranous positivity in glandular cells." /> </a> </div> <div class="alt-caption padding_left5" >Immunohistochemistry-Paraffin: PARD3/Par3 Antibody [NBP1-88861] - Staining of human colon shows distinct luminal membranous positivity in glandular cells.</div> </div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize/PARD3-Par3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88861-img0023.jpg" class="top_images" rel="gallery" title="Immunocytochemistry/Immunofluorescence: PARD3/Par3 Antibody [NBP1-88861] - MCAM is required to establish cell autonomous polarity. In wild-type myotubes PAR3 remains cytoplasmic. MCAM contributes to the establishment of cell autonomous polarity in myogenic and chondrogenic differentiation. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28923978/) licensed under a CC-BY license." > <img src="//images.novusbio.com/images/PARD3-Par3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88861-img0023.jpg" loading="lazy" style="display:none;" class="big_thumb" alt="Immunocytochemistry/Immunofluorescence: PARD3/Par3 Antibody [NBP1-88861] - MCAM is required to establish cell autonomous polarity. In wild-type myotubes PAR3 remains cytoplasmic. MCAM contributes to the establishment of cell autonomous polarity in myogenic and chondrogenic differentiation. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28923978/) licensed under a CC-BY license." title="Immunocytochemistry/Immunofluorescence: PARD3/Par3 Antibody [NBP1-88861] - MCAM is required to establish cell autonomous polarity. In wild-type myotubes PAR3 remains cytoplasmic. MCAM contributes to the establishment of cell autonomous polarity in myogenic and chondrogenic differentiation. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28923978/) licensed under a CC-BY license." /> </a> </div> <div class="alt-caption padding_left5" >Immunocytochemistry/ Immunofluorescence: PARD3/Par3 Antibody [NBP1-88861] - MCAM is required to establish cell autonomous polarity. In wild-type myotubes PAR3 remains cytoplasmic. MCAM contributes to the establishment<a class="openGallery" href="#" data-picnum="5"> ...read more</a></div> </div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize/PARD3-Par3-Antibody-Immunohistochemistry-Paraffin-NBP1-88861-img0022.jpg" class="top_images" rel="gallery" title="Immunohistochemistry-Paraffin: PARD3/Par3 Antibody [NBP1-88861] - Staining of human skin shows weak to moderate cytoplasmic positivity in epidermal cells." > <img src="//images.novusbio.com/images/PARD3-Par3-Antibody-Immunohistochemistry-Paraffin-NBP1-88861-img0022.jpg" loading="lazy" style="display:none;" class="big_thumb" alt="Immunohistochemistry-Paraffin: PARD3/Par3 Antibody [NBP1-88861] - Staining of human skin shows weak to moderate cytoplasmic positivity in epidermal cells." title="Immunohistochemistry-Paraffin: PARD3/Par3 Antibody [NBP1-88861] - Staining of human skin shows weak to moderate cytoplasmic positivity in epidermal cells." /> </a> </div> <div class="alt-caption padding_left5" >Immunohistochemistry-Paraffin: PARD3/Par3 Antibody [NBP1-88861] - Staining of human skin shows weak to moderate cytoplasmic positivity in epidermal cells.</div> </div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize/PARD3-Par3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88861-img0013.jpg" class="top_images" rel="gallery" title="Immunocytochemistry/Immunofluorescence: PARD3/Par3 Antibody [NBP1-88861] - Staining of human cell line 8505C cells (anaplastic thyroid cancer cell line). Image from verified customer review." > <img src="//images.novusbio.com/images/PARD3-Par3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88861-img0013.jpg" loading="lazy" style="display:none;" class="big_thumb" alt="Immunocytochemistry/Immunofluorescence: PARD3/Par3 Antibody [NBP1-88861] - Staining of human cell line 8505C cells (anaplastic thyroid cancer cell line). Image from verified customer review." title="Immunocytochemistry/Immunofluorescence: PARD3/Par3 Antibody [NBP1-88861] - Staining of human cell line 8505C cells (anaplastic thyroid cancer cell line). Image from verified customer review." /> </a> </div> <div class="alt-caption padding_left5" >Immunocytochemistry/ Immunofluorescence: PARD3/Par3 Antibody [NBP1-88861] - Staining of human cell line 8505C cells (anaplastic thyroid cancer cell line). Image from verified customer review.</div> </div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize/PARD3-Par3-Antibody-Immunohistochemistry-Paraffin-NBP1-88861-img0019.jpg" class="top_images" rel="gallery" title="Immunohistochemistry-Paraffin: PARD3/Par3 Antibody [NBP1-88861] - Staining of human kidney shows weak to moderate membranous positivity in cells in tubules." > <img src="//images.novusbio.com/images/PARD3-Par3-Antibody-Immunohistochemistry-Paraffin-NBP1-88861-img0019.jpg" loading="lazy" style="display:none;" class="big_thumb" alt="Immunohistochemistry-Paraffin: PARD3/Par3 Antibody [NBP1-88861] - Staining of human kidney shows weak to moderate membranous positivity in cells in tubules." title="Immunohistochemistry-Paraffin: PARD3/Par3 Antibody [NBP1-88861] - Staining of human kidney shows weak to moderate membranous positivity in cells in tubules." /> </a> </div> <div class="alt-caption padding_left5" >Immunohistochemistry-Paraffin: PARD3/Par3 Antibody [NBP1-88861] - Staining of human kidney shows weak to moderate membranous positivity in cells in tubules.</div> </div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize/PARD3-Par3-Antibody-Immunohistochemistry-Paraffin-NBP1-88861-img0020.jpg" class="top_images" rel="gallery" title="Immunohistochemistry-Paraffin: PARD3/Par3 Antibody [NBP1-88861] - Staining of human cerebellum shows moderate positivity in neuropil." > <img src="//images.novusbio.com/images/PARD3-Par3-Antibody-Immunohistochemistry-Paraffin-NBP1-88861-img0020.jpg" loading="lazy" style="display:none;" class="big_thumb" alt="Immunohistochemistry-Paraffin: PARD3/Par3 Antibody [NBP1-88861] - Staining of human cerebellum shows moderate positivity in neuropil." title="Immunohistochemistry-Paraffin: PARD3/Par3 Antibody [NBP1-88861] - Staining of human cerebellum shows moderate positivity in neuropil." /> </a> </div> <div class="alt-caption padding_left5" >Immunohistochemistry-Paraffin: PARD3/Par3 Antibody [NBP1-88861] - Staining of human cerebellum shows moderate positivity in neuropil.</div> </div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize/PARD3-Par3-Antibody-Immunohistochemistry-Paraffin-NBP1-88861-img0021.jpg" class="top_images" rel="gallery" title="Immunohistochemistry-Paraffin: PARD3/Par3 Antibody [NBP1-88861] - Staining of human fallopian tube shows weak to moderate positivity in luminal membrane in glandular cells." > <img src="//images.novusbio.com/images/PARD3-Par3-Antibody-Immunohistochemistry-Paraffin-NBP1-88861-img0021.jpg" loading="lazy" style="display:none;" class="big_thumb" alt="Immunohistochemistry-Paraffin: PARD3/Par3 Antibody [NBP1-88861] - Staining of human fallopian tube shows weak to moderate positivity in luminal membrane in glandular cells." title="Immunohistochemistry-Paraffin: PARD3/Par3 Antibody [NBP1-88861] - Staining of human fallopian tube shows weak to moderate positivity in luminal membrane in glandular cells." /> </a> </div> <div class="alt-caption padding_left5" >Immunohistochemistry-Paraffin: PARD3/Par3 Antibody [NBP1-88861] - Staining of human fallopian tube shows weak to moderate positivity in luminal membrane in glandular cells.</div> </div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize/PARD3-Par3-Antibody-Simple-Western-NBP1-88861-img0015.jpg" class="top_images" rel="gallery" title="Simple Western: PARD3/Par3 Antibody [NBP1-88861] - Electropherogram image(s) of corresponding Simple Western lane view. PARD3/Par3 antibody was used at 1:100 dilution on RT-4 lysate(s)." > <img src="//images.novusbio.com/images/PARD3-Par3-Antibody-Simple-Western-NBP1-88861-img0015.jpg" loading="lazy" style="display:none;" class="big_thumb" alt="Simple Western: PARD3/Par3 Antibody [NBP1-88861] - Electropherogram image(s) of corresponding Simple Western lane view. PARD3/Par3 antibody was used at 1:100 dilution on RT-4 lysate(s)." title="Simple Western: PARD3/Par3 Antibody [NBP1-88861] - Electropherogram image(s) of corresponding Simple Western lane view. PARD3/Par3 antibody was used at 1:100 dilution on RT-4 lysate(s)." /> </a> </div> <div class="alt-caption padding_left5" >Simple Western: PARD3/Par3 Antibody [NBP1-88861] - Electropherogram image(s) of corresponding Simple Western lane view. PARD3/Par3 antibody was used at 1:100 dilution on RT-4 lysate(s).</div> </div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize/nbp1-88861_rabbit-polyclonal-pard3-par3-antibody-310202416113535.jpg" class="top_images" rel="gallery" title="Immunocytochemistry/ Immunofluorescence: PARD3/Par3 Antibody [NBP1-88861] - MCAM is required to establish cell autonomous polarity. (A) In elongating myotubes (10T1/2 cells treated with testosterone for 7 days) VANGL2 is localized asymmetrically at the tip of the cell. (B) The VANGL2 enriched tip of the cell is marked by MSN. (C) In MCAM knockout C164 cells myotube elongation fails, MSN labels the whole plasma membrane & VANGL2 is spread across the cytoplasm. (D) Highly polarized localization of MCAM & SCRIB at the distal end of growing wild-type myotube. Separate channels of the boxed area are shown on the right. (E) In MCAM knockout cells SCRIB levels remain low & it is spread evenly in the cell. (F) In wild-type myotubes PAR3 remains cytoplasmic, whereas (G) in MCAM knockout C164 cells it can be detected at the cell cortex. (H) RT-qPCR demonstrates reduced expression of Scrib in MCAM mutant cell lines. Cells were treated for 7 days with BMP2 or testosterone (n=3; **P<0.01; ***P<0.001; two-tailed t-test; mean卤s.e.m.). (I) Deletion of MCAM endocytosis motif leads to similar polarity defects as complete MCAM elimination. VANGL2 is evenly spread in U125 cells & PAR3 accumulates in cell cortex. (J) In chondrogenic differentiation VANGL2 was observed asymmetrically in limited number of cells. In MCAM mutant cell lines (C149, C164, U125) VANGL2 accumulated around the nucleus. (K) Initiation of myogenic (4-day culture with testosterone) & chondrogenic differentiation (4-day culture with BMP2) led to downregulation of ERK1/2 phosphorylation (p-ERK1/2). Instead in MCAM mutant cell lines ERK1/2 phosphorylation increased. Scale bars: 25 碌m. Image collected & cropped by CiteAb from the following publication (//journals.biologists.com/bio/article/doi/10.1242/bio.027771/256759/MCAM-contributes-to-the-establishment-of-cell), licensed under a CC-BY license. Not internally tested by Novus Biologicals." > <img src="//images.novusbio.com/images/nbp1-88861_rabbit-polyclonal-pard3-par3-antibody-310202416113535.jpg" loading="lazy" style="display:none;" class="big_thumb" alt="Immunocytochemistry/ Immunofluorescence: PARD3/Par3 Antibody [NBP1-88861] - MCAM is required to establish cell autonomous polarity. (A) In elongating myotubes (10T1/2 cells treated with testosterone for 7 days) VANGL2 is localized asymmetrically at the tip of the cell. (B) The VANGL2 enriched tip of the cell is marked by MSN. (C) In MCAM knockout C164 cells myotube elongation fails, MSN labels the whole plasma membrane & VANGL2 is spread across the cytoplasm. (D) Highly polarized localization of MCAM & SCRIB at the distal end of growing wild-type myotube. Separate channels of the boxed area are shown on the right. (E) In MCAM knockout cells SCRIB levels remain low & it is spread evenly in the cell. (F) In wild-type myotubes PAR3 remains cytoplasmic, whereas (G) in MCAM knockout C164 cells it can be detected at the cell cortex. (H) RT-qPCR demonstrates reduced expression of Scrib in MCAM mutant cell lines. Cells were treated for 7 days with BMP2 or testosterone (n=3; **P<0.01; ***P<0.001; two-tailed t-test; mean卤s.e.m.). (I) Deletion of MCAM endocytosis motif leads to similar polarity defects as complete MCAM elimination. VANGL2 is evenly spread in U125 cells & PAR3 accumulates in cell cortex. (J) In chondrogenic differentiation VANGL2 was observed asymmetrically in limited number of cells. In MCAM mutant cell lines (C149, C164, U125) VANGL2 accumulated around the nucleus. (K) Initiation of myogenic (4-day culture with testosterone) & chondrogenic differentiation (4-day culture with BMP2) led to downregulation of ERK1/2 phosphorylation (p-ERK1/2). Instead in MCAM mutant cell lines ERK1/2 phosphorylation increased. Scale bars: 25 碌m. Image collected & cropped by CiteAb from the following publication (//journals.biologists.com/bio/article/doi/10.1242/bio.027771/256759/MCAM-contributes-to-the-establishment-of-cell), licensed under a CC-BY license. Not internally tested by Novus Biologicals." title="Immunocytochemistry/ Immunofluorescence: PARD3/Par3 Antibody [NBP1-88861] - MCAM is required to establish cell autonomous polarity. (A) In elongating myotubes (10T1/2 cells treated with testosterone for 7 days) VANGL2 is localized asymmetrically at the tip of the cell. (B) The VANGL2 enriched tip of the cell is marked by MSN. (C) In MCAM knockout C164 cells myotube elongation fails, MSN labels the whole plasma membrane & VANGL2 is spread across the cytoplasm. (D) Highly polarized localization of MCAM & SCRIB at the distal end of growing wild-type myotube. Separate channels of the boxed area are shown on the right. (E) In MCAM knockout cells SCRIB levels remain low & it is spread evenly in the cell. (F) In wild-type myotubes PAR3 remains cytoplasmic, whereas (G) in MCAM knockout C164 cells it can be detected at the cell cortex. (H) RT-qPCR demonstrates reduced expression of Scrib in MCAM mutant cell lines. Cells were treated for 7 days with BMP2 or testosterone (n=3; **P<0.01; ***P<0.001; two-tailed t-test; mean卤s.e.m.). (I) Deletion of MCAM endocytosis motif leads to similar polarity defects as complete MCAM elimination. VANGL2 is evenly spread in U125 cells & PAR3 accumulates in cell cortex. (J) In chondrogenic differentiation VANGL2 was observed asymmetrically in limited number of cells. In MCAM mutant cell lines (C149, C164, U125) VANGL2 accumulated around the nucleus. (K) Initiation of myogenic (4-day culture with testosterone) & chondrogenic differentiation (4-day culture with BMP2) led to downregulation of ERK1/2 phosphorylation (p-ERK1/2). Instead in MCAM mutant cell lines ERK1/2 phosphorylation increased. Scale bars: 25 碌m. Image collected & cropped by CiteAb from the following publication (//journals.biologists.com/bio/article/doi/10.1242/bio.027771/256759/MCAM-contributes-to-the-establishment-of-cell), licensed under a CC-BY license. Not internally tested by Novus Biologicals." /> </a> </div> <div class="alt-caption padding_left5" >Immunocytochemistry/ Immunofluorescence: PARD3/Par3 Antibody [NBP1-88861] - MCAM is required to establish cell autonomous polarity. (A) In elongating myotubes (10T1/2 cells treated with testosterone for 7 days) VANGL2<a class="openGallery" href="#" data-picnum="12"> ...read more</a></div> </div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize/nbp1-88861_rabbit-polyclonal-pard3-par3-antibody-310202416113539.jpg" class="top_images" rel="gallery" title="Immunocytochemistry/ Immunofluorescence: PARD3/Par3 Antibody [NBP1-88861] - MCAM is required to establish cell autonomous polarity. (A) In elongating myotubes (10T1/2 cells treated with testosterone for 7 days) VANGL2 is localized asymmetrically at the tip of the cell. (B) The VANGL2 enriched tip of the cell is marked by MSN. (C) In MCAM knockout C164 cells myotube elongation fails, MSN labels the whole plasma membrane & VANGL2 is spread across the cytoplasm. (D) Highly polarized localization of MCAM & SCRIB at the distal end of growing wild-type myotube. Separate channels of the boxed area are shown on the right. (E) In MCAM knockout cells SCRIB levels remain low & it is spread evenly in the cell. (F) In wild-type myotubes PAR3 remains cytoplasmic, whereas (G) in MCAM knockout C164 cells it can be detected at the cell cortex. (H) RT-qPCR demonstrates reduced expression of Scrib in MCAM mutant cell lines. Cells were treated for 7 days with BMP2 or testosterone (n=3; **P<0.01; ***P<0.001; two-tailed t-test; mean卤s.e.m.). (I) Deletion of MCAM endocytosis motif leads to similar polarity defects as complete MCAM elimination. VANGL2 is evenly spread in U125 cells & PAR3 accumulates in cell cortex. (J) In chondrogenic differentiation VANGL2 was observed asymmetrically in limited number of cells. In MCAM mutant cell lines (C149, C164, U125) VANGL2 accumulated around the nucleus. (K) Initiation of myogenic (4-day culture with testosterone) & chondrogenic differentiation (4-day culture with BMP2) led to downregulation of ERK1/2 phosphorylation (p-ERK1/2). Instead in MCAM mutant cell lines ERK1/2 phosphorylation increased. Scale bars: 25 碌m. Image collected & cropped by CiteAb from the following publication (//journals.biologists.com/bio/article/doi/10.1242/bio.027771/256759/MCAM-contributes-to-the-establishment-of-cell), licensed under a CC-BY license. Not internally tested by Novus Biologicals." > <img src="//images.novusbio.com/images/nbp1-88861_rabbit-polyclonal-pard3-par3-antibody-310202416113539.jpg" loading="lazy" style="display:none;" class="big_thumb" alt="Immunocytochemistry/ Immunofluorescence: PARD3/Par3 Antibody [NBP1-88861] - MCAM is required to establish cell autonomous polarity. (A) In elongating myotubes (10T1/2 cells treated with testosterone for 7 days) VANGL2 is localized asymmetrically at the tip of the cell. (B) The VANGL2 enriched tip of the cell is marked by MSN. (C) In MCAM knockout C164 cells myotube elongation fails, MSN labels the whole plasma membrane & VANGL2 is spread across the cytoplasm. (D) Highly polarized localization of MCAM & SCRIB at the distal end of growing wild-type myotube. Separate channels of the boxed area are shown on the right. (E) In MCAM knockout cells SCRIB levels remain low & it is spread evenly in the cell. (F) In wild-type myotubes PAR3 remains cytoplasmic, whereas (G) in MCAM knockout C164 cells it can be detected at the cell cortex. (H) RT-qPCR demonstrates reduced expression of Scrib in MCAM mutant cell lines. Cells were treated for 7 days with BMP2 or testosterone (n=3; **P<0.01; ***P<0.001; two-tailed t-test; mean卤s.e.m.). (I) Deletion of MCAM endocytosis motif leads to similar polarity defects as complete MCAM elimination. VANGL2 is evenly spread in U125 cells & PAR3 accumulates in cell cortex. (J) In chondrogenic differentiation VANGL2 was observed asymmetrically in limited number of cells. In MCAM mutant cell lines (C149, C164, U125) VANGL2 accumulated around the nucleus. (K) Initiation of myogenic (4-day culture with testosterone) & chondrogenic differentiation (4-day culture with BMP2) led to downregulation of ERK1/2 phosphorylation (p-ERK1/2). Instead in MCAM mutant cell lines ERK1/2 phosphorylation increased. Scale bars: 25 碌m. Image collected & cropped by CiteAb from the following publication (//journals.biologists.com/bio/article/doi/10.1242/bio.027771/256759/MCAM-contributes-to-the-establishment-of-cell), licensed under a CC-BY license. Not internally tested by Novus Biologicals." title="Immunocytochemistry/ Immunofluorescence: PARD3/Par3 Antibody [NBP1-88861] - MCAM is required to establish cell autonomous polarity. (A) In elongating myotubes (10T1/2 cells treated with testosterone for 7 days) VANGL2 is localized asymmetrically at the tip of the cell. (B) The VANGL2 enriched tip of the cell is marked by MSN. (C) In MCAM knockout C164 cells myotube elongation fails, MSN labels the whole plasma membrane & VANGL2 is spread across the cytoplasm. (D) Highly polarized localization of MCAM & SCRIB at the distal end of growing wild-type myotube. Separate channels of the boxed area are shown on the right. (E) In MCAM knockout cells SCRIB levels remain low & it is spread evenly in the cell. (F) In wild-type myotubes PAR3 remains cytoplasmic, whereas (G) in MCAM knockout C164 cells it can be detected at the cell cortex. (H) RT-qPCR demonstrates reduced expression of Scrib in MCAM mutant cell lines. Cells were treated for 7 days with BMP2 or testosterone (n=3; **P<0.01; ***P<0.001; two-tailed t-test; mean卤s.e.m.). (I) Deletion of MCAM endocytosis motif leads to similar polarity defects as complete MCAM elimination. VANGL2 is evenly spread in U125 cells & PAR3 accumulates in cell cortex. (J) In chondrogenic differentiation VANGL2 was observed asymmetrically in limited number of cells. In MCAM mutant cell lines (C149, C164, U125) VANGL2 accumulated around the nucleus. (K) Initiation of myogenic (4-day culture with testosterone) & chondrogenic differentiation (4-day culture with BMP2) led to downregulation of ERK1/2 phosphorylation (p-ERK1/2). Instead in MCAM mutant cell lines ERK1/2 phosphorylation increased. Scale bars: 25 碌m. Image collected & cropped by CiteAb from the following publication (//journals.biologists.com/bio/article/doi/10.1242/bio.027771/256759/MCAM-contributes-to-the-establishment-of-cell), licensed under a CC-BY license. Not internally tested by Novus Biologicals." /> </a> </div> <div class="alt-caption padding_left5" >Immunocytochemistry/ Immunofluorescence: PARD3/Par3 Antibody [NBP1-88861] - MCAM is required to establish cell autonomous polarity. (A) In elongating myotubes (10T1/2 cells treated with testosterone for 7 days) VANGL2<a class="openGallery" href="#" data-picnum="13"> ...read more</a></div> </div></div><div id="ss_next_top" class="cycle-pager"><div class="pager_right_on"></div></div></div></div><div class="productsright pos_relative equaltop"><h2 class="blueHead">Product Details</h2> <div class="midBlue">Summary</div><table class="ds_list"><tr class="lined"> <td><strong>Reactivity</strong></td> <td><span title='Human'>Hu</span>, <span title='Mouse'>Mu</span><span class="myModal">Species Glossary</span></td> </tr><tr class="lined"> <td><strong>Applications</strong></td> <td><span title='Western Blot'>WB</span>, <span title='Simple Western'>Simple Western</span>, <span title='Immunocytochemistry/ Immunofluorescence'>ICC/IF</span>, <span title='Immunohistochemistry'>IHC</span>, <span title='Immunoprecipitation'>IP</span></td> </tr><tr class="lined"> <td><strong>Clonality</strong></td> <td><div>Polyclonal</div></td> </tr><tr class="lined"> <td><strong>Host</strong></td> <td><div>Rabbit</div></td> </tr><tr class="lined"> <td><strong>Conjugate</strong></td> <td><div>Unconjugated</div></td> </tr></table></div><div class="AtlasDSDiv" style="clear:left;"><div class="AtlasDSValidatedText">Validated by: </div>      <span class="AtlasDSSpan"><a href="/5-pillars-validation" class="AtlasDSOrthogonal" title="Orthogonal Strategies"> </a><p><a href="/5-pillars-validation" title="Orthogonal Strategies">Orthogonal Strategies</a></p></span>  </div> </div></div><div class="grid_6"><div></div> <div class="ds_blue_border equaltop"> <h2 class="blueHead_nopadding">Order Details</h2><ul class="ds_details"><li class="add-to-cart" data-type="full" data-prodname="" data-catnum="NBP1-88861" data-catnums="NBP1-88861,NBP1-88861-25ul" data-disabled=''><noscript><form autocomplete="off" action="/products/pard3-par3-antibody_nbp1-88861" method="post" id="et-add-multiple-to-cart-form" accept-charset="UTF-8"><div><table class="sticky-enabled"> <thead><tr><th>Size</th><th>Price</th> </tr></thead> <tbody> <tr class="odd"><td><div class="atc_size" title="catalog # NBP1-88861-25ul">25ul</div><div data-msg='' class="atc_catnum">NBP1-88861-25ul</div></td><td><div style="font-size:11px;"><a class="jump_distributor" href="#jump_distributor">Contact Your<br />Distributor</a></div></td> </tr> <tr class="even"><td colspan="3" class="stock_row">In Stock: 3-5 Business Days</td> </tr> <tr class="odd"><td><div class="atc_size" title="catalog # NBP1-88861">0.1 ml</div><div data-msg='' class="atc_catnum">NBP1-88861</div></td><td><div style="font-size:11px;"><a class="jump_distributor" href="#jump_distributor">Contact Your<br />Distributor</a></div></td> </tr> <tr class="even"><td colspan="3" class="stock_row">In Stock: 3-5 Business Days</td> </tr> </tbody> </table> <input type="hidden" name="form_build_id" value="form-0mZfhdrrNYheU_kf87ovTBkn_hbjXgkhV9O7tOIh1Mk" /> <input type="hidden" name="form_id" value="et_add_multiple_to_cart_form" /> </div></form></noscript></li></ul><div id="shipping" class="hidden"></td></tr></div><div class="conj_form_buttons"></div><div id="shipping_statement"><span id="shipping_rate"></span></div><div class="shipping_obt_transfer"><span>Novus Biologicals is part of Bio-Techne</span><br><br><a href="https://www.bio-techne.com/p/antibodies/pard3-par3-antibody_nbp1-88861" rel="follow" class="btn btn-primary">Shop this product on bio-techne.com</a></div><br><div class="learn_more"> <a href="/guarantee"> <div class="sprite_noImage" title="Novus Biologicals, part of Bio-Techne, offers 100% Guarantee"></div> <div class="learn_more_text">Novus Biologicals, part of Bio-Techne, offers 100% Guarantee</div></a> </div><span id="conj_form" class="hidden"><tr><td colspan="4"></td></tr></span> </div> </div><div class="clear"></div><div class="grid_10"><div><div id="ds_tabs" style="display:none;"> <div class="ds_tab_active" id="dsTabs_datasheet" data-anchor_desc="datasheet">Datasheet</div> <div class="ds_tab" id="dsTabs_reviews-publications" data-anchor_desc="reviews-publications">Reviews & Publications</div> <div class="ds_tab" id="dsTabs_protocols-faqs" data-anchor_desc="protocols-faqs">Protocols & FAQs</div> <div class="ds_tab" id="dsTabs_supportresearch" data-anchor_desc="supportresearch">Support & Research</div> </div><div class="ds_tab_content equalbottom" id="content_datasheet" > <a id="datasheet"></a><h2 class=greyHead2>PARD3/Par3 Antibody Summary</h2> <div class="ds_info"> <a id="tab1details"></a> <table class="ds_list wide" style="table-layout:fixed;"> <tr class="lined"> <td><strong>Immunogen</strong></td> <td><div>This antibody was developed against Recombinant Protein corresponding to amino acids: LKGLGDMFRIQAKTREFRERQARERDYAEIQDFHRTFGCDDELMYGGVSSYEGSMALNARPQSPREGHMMDALYAQVKKPRNSKPSPVDSNR</div></td> </tr><tr class="lined"> <td><strong>Isotype</strong></td> <td><div>IgG</div></td> </tr><tr class="lined"> <td><strong>Clonality</strong></td> <td><div>Polyclonal</div></td> </tr><tr class="lined"> <td><strong>Host</strong></td> <td><div>Rabbit</div></td> </tr><tr class="lined"> <td><strong>Gene</strong></td> <td><div>PARD3</div></td> </tr><tr class="lined"> <td><strong>Purity</strong></td> <td><div>Immunogen affinity purified</div></td> </tr><tr class="lined"> <td><strong>Innovator's Reward</strong></td> <td><div>Test in a species/application not listed above to receive a full credit towards a future purchase.<br/><div class='text_right'><a class='ds_submit_blue ds_regularPad' href='/support/innovators-reward.html'>Learn about the Innovator's Reward</a></div></div></td> </tr> </table> </div> <h2 class="greyHead2">Applications/Dilutions</h2> <div class="ds_info"> <a id="tab1appsdiluts"></a> <table class="ds_list wide"> <tr class="lined"> <td><strong>Dilutions</strong></td> <td> <ul class="ds_noDotList"><li>Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml</li><li>Immunohistochemistry 1:200 - 1:500</li><li>Immunohistochemistry-Paraffin 1:200-1:500</li><li>Immunoprecipitation Reported in scientific literature (PMID:34158849).</li><li>Simple Western 1:1000</li><li>Western Blot 0.04-0.4 ug/ml</li> </ul> </td> </tr><tr class="lined"> <td><strong>Application Notes</strong></td> <td><div>ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. IHC-Paraffin HIER pH6 retrieval is recommended. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.</div></td> </tr> <tr class="lined"> <td><strong>Control Peptide</strong></td> <td> <table class="wide ds_altTable"><tr> <td style="width:80%"><a href="/pard3-par3-recombinant-protein-antigen_nbp1-88861pep">PARD3/Par3 Recombinant Protein Antigen (NBP1-88861PEP)</a></td> <td class="text_right"> <div class="add-to-cart" data-type="anc_btnonly" data-catbase="NBP1-88861PEP" data-return="pard3-par3-antibody_nbp1-88861" data-produrl="pard3-par3-antibody_nbp1-88861" data-catnums="NBP1-88861PEP" ></div> </td> </tr></table> </td> </tr><tr class="lined"> <td><strong>Reviewed Applications</strong></td> <td> <table class="wide"> <tr> <td> Read <a href="#ReviewsSection">2 Reviews</a> rated 4<div class="dsSprite_stars4"></div> using <br/>NBP1-88861 in the following applications:<br /><br /> <ul class="information-list"><li> <div class="information-subject"><a href="#ReviewsSection" class="filter_link" data-destination="review" data-type="application" data-filter="Immunofluorescence">Immunofluorescence</a></div> <div class="information-data"></div> </li><li> <div class="information-subject"><a href="#ReviewsSection" class="filter_link" data-destination="review" data-type="application" data-filter="Immunocytochemistry">Immunocytochemistry</a></div> <div class="information-data"></div> </li> </ul> </td> </tr> </table> </td> </tr><tr class="lined"> <td><strong>Publications</strong></td> <td> <table> <tr> <td>Read <a href="#PublicationSection"> Publications</a> using<br/> NBP1-88861 in the following applications:<br /><br /> <ul class="information-list"><li class="noIndent"><div class="information-subject"> <a href="#PublicationSection" class="filter_link" data-destination="publication" data-type="application" data-filter="ICC/IF">ICC/IF</a> </div><div class="information-data">3 publications</div></li><li class="noIndent"><div class="information-subject"> <a href="#PublicationSection" class="filter_link" data-destination="publication" data-type="application" data-filter="IF/IHC">IF/IHC</a> </div><div class="information-data">2 publications</div></li><li class="noIndent"><div class="information-subject"> <a href="#PublicationSection" class="filter_link" data-destination="publication" data-type="application" data-filter="IHC-Fr">IHC-Fr</a> </div><div class="information-data">1 publication</div></li><li class="noIndent"><div class="information-subject"> <a href="#PublicationSection" class="filter_link" data-destination="publication" data-type="application" data-filter="IHC-P">IHC-P</a> </div><div class="information-data">1 publication</div></li><li class="noIndent"><div class="information-subject"> <a href="#PublicationSection" class="filter_link" data-destination="publication" data-type="application" data-filter="IP">IP</a> </div><div class="information-data">1 publication</div></li></ul></td> </tr> </table> </td> </tr> </table> </div><h2 class="greyHead2">Reactivity Notes</h2> <div> <a id="tab1speciesreactivity"></a> <div class="ds_info"> Mouse reactivity reported from a verified customer review.</div> </div> <h2 class="greyHead2">Packaging, Storage & Formulations</h2><div class="ds_info"><table class="ds_list"><tr class="lined"> <td><strong>Storage</strong></td> <td><div>Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.</div></td> </tr><tr class="lined"> <td><strong>Buffer</strong></td> <td><div>PBS (pH 7.2) and 40% Glycerol</div></td> </tr><tr class="lined"> <td><strong>Preservative</strong></td> <td><div>0.02% Sodium Azide</div></td> </tr><tr class="lined"> <td><strong>Purity</strong></td> <td><div>Immunogen affinity purified</div></td> </tr></table></div><h2 class="greyHead2">Alternate Names for PARD3/Par3 Antibody</h2> <div class="ds_info"> <a id="tab1altnames"></a> <div class="information-list"> <ul><li>ASIP</li><li>Baz</li><li>Bazooka</li><li>FLJ21015</li><li>par-3 partitioning defective 3 homolog (C. elegans)</li><li>PAR3</li><li>PAR3A</li><li>PAR3C.elegans) homolog</li><li>PARD3</li><li>SE2-5L16</li><li>SE2-5LT1</li><li>SE2-5T2</li></ul></div> </div> <h2 class="greyHead2">Background</h2> <div class="ds_info"> <a id="tab1background"></a> <div>PARD3 is an adaptor protein involved in asymmetrical cell division and cell polarization processes. PARD3 seems to play a central role in the formation of epithelial tight junctions. PARD3 may affect the quality of hair pigmentation, and act as a pharmacological antagonist of alpha-melanocyte-stimulating hormone (2-3). PARD3 may also play a role in neuroendocrine aspects of melanocortin action, and have a functional role in regulating lipid metabolism in adipocytes (4-5). Recent studies have suggested PARD3 (Par-3) is essential in the formation of myelin sheaths on neuronal axons.</div> </div> <h2 class="greyHead2">Limitations</h2> <div class="ds_info"> <a id="tab1background"></a> <div>This product is for research use only and is not approved for use in humans or in clinical diagnosis. 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width: 280px;" title="PAR1/Thrombin Receptor Antibody (N2-11)">PAR1/Thrombin<br> Receptor Antibody (N2</a>    </div><div class="sub-header">NBP1-71770</div> <div class="images_customers"><div id="ss_prev_cav4" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-4" data-cycle-prev="#ss_prev_cav4" data-cycle-next="#ss_next_cav4" data-cycle-slides="div.big_thumb_cust_container" data-index=4 ><div class="big_thumb_cust_container"> <a href="/products/par1-thrombin-receptor-antibody-n2-11_nbp1-71770" title="Western Blot: PAR1/Thrombin Receptor Antibody (N2-11) [NBP1-71770] - Analysis of PAR1 in whole cell lysate of Neuro2A cell line using 4ug/ml concentration of PAR1 antibody (clone N2-11). The antibody detected a strong band at ~60kDa position and another light band at ~80kDa, both of which appears to be the glycosylated forms of PAR1 protein." class="cust_viewed_4" > <img loading="lazy" src="https://images.novusbio.com/images/PAR1-Thrombin-Receptor-Antibody-N2-11-Western-Blot-NBP1-71770-img0010.jpg" alt="Western Blot PAR1/Thrombin Receptor Antibody (N2-11)" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/par1-thrombin-receptor-antibody-n2-11_nbp1-71770" title="Immunocytochemistry/Immunofluorescence: PAR1/Thrombin Receptor Antibody (N2-11) [NBP1-71770] - Thrombin Receptor antibody was tested in HeLa cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red)." class="cust_viewed_4" > <img loading="lazy" src="https://images.novusbio.com/images/PAR1-Thrombin-Receptor-Antibody-N2-11-Immunocytochemistry-Immunofluorescence-NBP1-71770-img0011.jpg" alt="Immunocytochemistry/ Immunofluorescence PAR1/Thrombin Receptor Antibody (N2-11)" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/par1-thrombin-receptor-antibody-n2-11_nbp1-71770" title="Immunohistochemistry: PAR1/Thrombin Receptor Antibody (N2-11) [NBP1-71770] - Analysis of Thrombin Receptor on mouse skin using NBP1-71770." class="cust_viewed_4" > <img loading="lazy" src="https://images.novusbio.com/images/PAR1-Thrombin-Receptor-Antibody-N2-11-Immunohistochemistry-NBP1-71770-img0012.jpg" alt="Immunohistochemistry PAR1/Thrombin Receptor Antibody (N2-11)" class="big_thumb" /> </a> </div></div><div id="ss_next_cav4" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-4" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/par1-thrombin-receptor-antibody-n2-11_nbp1-71770#PublicationSection"> <div class="sprite_pubBook"></div> (10 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu, Mu</div><div class="tiny"><strong>Applications:</strong> ELISA, ICC/IF, IF, IHC, IHC-Fr, IHC-P, WB</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NBP2-49382"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/gpr172a-antibody_nbp2-49382" style="overflow: hidden; width: 280px;" title="GPR172A Antibody">GPR172A Antibody</a>    </div><div class="sub-header">NBP2-49382</div> <div class="images_customers"><div class="one_image_l"></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-5" data-cycle-prev="#ss_prev_cav5" data-cycle-next="#ss_next_cav5" data-cycle-slides="div.big_thumb_cust_container" data-index=5 ><div class="big_thumb_cust_container"> <a href="/products/gpr172a-antibody_nbp2-49382" title="Immunohistochemistry: GPR172A Antibody [NBP2-49382] - Staining of human small intestine shows moderate cytoplasmic and membranous positivity in glandular cells." class="cust_viewed_5" > <img loading="lazy" src="https://images.novusbio.com/images/GPR172A-Antibody-Immunohistochemistry-NBP2-49382-img0001.jpg" alt="Immunohistochemistry GPR172A Antibody" class="big_thumb" /> </a> </div></div><div class="one_image"></div><div class="one_image_11"></div></div> <div class="ds_info"><div class="tiny"><strong>Species:</strong> Hu</div><div class="tiny"><strong>Applications:</strong> IHC, IHC-P</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NBP3-25558"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/melanocortin-1-r-mc1r-antibody-hl1471_nbp3-25558" style="overflow: hidden; width: 280px;" title="Melanocortin-1 R/MC1R Antibody (HL1471) - Azide and BSA Free">Melanocortin-1<br> R/MC1R Antibody (HL14 ...</a>    </div><div class="sub-header">NBP3-25558</div> <div class="images_customers"><div id="ss_prev_cav6" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-6" data-cycle-prev="#ss_prev_cav6" data-cycle-next="#ss_next_cav6" data-cycle-slides="div.big_thumb_cust_container" data-index=6 ><div class="big_thumb_cust_container"> <a href="/products/melanocortin-1-r-mc1r-antibody-hl1471_nbp3-25558" title="Immunohistochemistry-Paraffin: Melanocortin-1 R/MC1R Antibody (HL1471) - Azide and BSA Free [NBP3-25558] - MC1 Receptor antibody [HL1471] detects MC1 Receptor protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. MC1 Receptor stained by MC1 Receptor antibody [HL1471] (NBP3-25558) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min" class="cust_viewed_6" > <img loading="lazy" src="https://images.novusbio.com/images/nbp3-25558_rabbit-melanocortin-1-r-mc1r-mab-hl1471-azide-bsa-free-2120249432833.jpg" alt="Immunohistochemistry-Paraffin Melanocortin-1 R/MC1R Antibody (HL1471) - Azide and BSA Free" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/melanocortin-1-r-mc1r-antibody-hl1471_nbp3-25558" title="Immunohistochemistry-Paraffin: Melanocortin-1 R/MC1R Antibody (HL1471) - Azide and BSA Free [NBP3-25558] - MC1 Receptor antibody [HL1471] detects MC1 Receptor protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. MC1 Receptor stained by MC1 Receptor antibody [HL1471] (NBP3-25558) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min" class="cust_viewed_6" > <img loading="lazy" src="https://images.novusbio.com/images/nbp3-25558_rabbit-melanocortin-1-r-mc1r-mab-hl1471-azide-bsa-free-2120249415999.jpg" alt="Immunohistochemistry-Paraffin Melanocortin-1 R/MC1R Antibody (HL1471) - Azide and BSA Free" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/melanocortin-1-r-mc1r-antibody-hl1471_nbp3-25558" title="Knockdown Validated: Melanocortin-1 R/MC1R Antibody (HL1471) - Azide and BSA Free [NBP3-25558] - Non-transfected (-) and transfected (+) A375 whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with MC1 Receptor antibody [HL1471] (NBP3-25558) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody." class="cust_viewed_6" > <img loading="lazy" src="https://images.novusbio.com/images/nbp3-25558_rabbit-melanocortin-1-r-mc1r-mab-hl1471-azide-bsa-free-2120249351383.jpg" alt="Knockdown Validated Melanocortin-1 R/MC1R Antibody (HL1471) - Azide and BSA Free" class="big_thumb" /> </a> </div></div><div id="ss_next_cav6" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-6" class="cycle-circle-pager"></div></div> <div class="ds_info"><div class="tiny"><strong>Species:</strong> Hu, Mu, Rt</div><div class="tiny"><strong>Applications:</strong> ICC/IF, IHC, IHC-P, WB</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NB100-1533"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/pomc-antibody_nb100-1533" style="overflow: hidden; width: 280px;" title="POMC Antibody">POMC Antibody</a>    </div><div class="sub-header">NB100-1533</div> <div class="images_customers"><div id="ss_prev_cav7" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-7" data-cycle-prev="#ss_prev_cav7" data-cycle-next="#ss_next_cav7" data-cycle-slides="div.big_thumb_cust_container" data-index=7 ><div class="big_thumb_cust_container"> <a href="/products/pomc-antibody_nb100-1533" title="Flow Cytometry: POMC Antibody [NB100-1533] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody." class="cust_viewed_7" > <img loading="lazy" src="https://images.novusbio.com/images/POMC-Antibody-Flow-Cytometry-NB100-1533-img0006.jpg" alt="Flow Cytometry POMC Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/pomc-antibody_nb100-1533" title="Immunohistochemistry: POMC Antibody [NB100-1533] - Representative immunofluorescence images of (B) POMC as a prohormone (n = 5-6 each) in the ARC of mice approximately 5 to 10 weeks of age. Image collected and cropped by CiteAb from the following publication (jci.org/articles/view/96420), licensed under a CC-BY license." class="cust_viewed_7" > <img loading="lazy" src="https://images.novusbio.com/images/POMC-Antibody-Immunohistochemistry-NB100-1533-img0007.jpg" alt="Immunohistochemistry POMC Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/pomc-antibody_nb100-1533" title="Immunohistochemistry: POMC Antibody [NB100-1533] - Representative confocal images of POMC in POMC-transfected WT and Sel1L-/- N2a cells. White arrows point to POMC-containing secretory granules, while yellow arrows point to perinuclear POMC. KDEL marks the ER. Representative data from at least 2 independent experiments are shown. Image collected and cropped by CiteAb from the following publication (jci.org/articles/view/96420), licensed under a CC-BY license." class="cust_viewed_7" > <img loading="lazy" src="https://images.novusbio.com/images/POMC-Antibody-Immunohistochemistry-NB100-1533-img0008.jpg" alt="Immunohistochemistry POMC Antibody" class="big_thumb" /> </a> </div></div><div id="ss_next_cav7" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-7" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/pomc-antibody_nb100-1533#PublicationSection"> <div class="sprite_pubBook"></div> (10 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu, Mu, Po, Rt, Sh</div><div class="tiny"><strong>Applications:</strong> Flow, ICC/IF, IHC, IHC-P, PEP-ELISA, WB</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NBP1-87338"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/pawr-par4-antibody_nbp1-87338" style="overflow: hidden; width: 280px;" title="PAWR / PAR4 Antibody">PAWR / PAR4<br> Antibody</a>    </div><div class="sub-header">NBP1-87338</div> <div class="images_customers"><div id="ss_prev_cav8" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-8" data-cycle-prev="#ss_prev_cav8" data-cycle-next="#ss_next_cav8" data-cycle-slides="div.big_thumb_cust_container" data-index=8 ><div class="big_thumb_cust_container"> <a href="/products/pawr-par4-antibody_nbp1-87338" title="Immunohistochemistry-Paraffin: PAWR / PAR4 Antibody [NBP1-87338] - Staining of human endometrium shows strong cytoplasmic positivity in glandular cells and smooth muscle cells." class="cust_viewed_8" > <img loading="lazy" src="https://images.novusbio.com/images/PAWR---PAR4-Antibody-Immunohistochemistry-Paraffin-NBP1-87338-img0012.jpg" alt="Immunohistochemistry-Paraffin PAWR / PAR4 Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/pawr-par4-antibody_nbp1-87338" title="Immunohistochemistry-Paraffin: PAWR / PAR4 Antibody [NBP1-87338] - Staining of human rectum shows strong cytoplasmic positivity in glandular cells." class="cust_viewed_8" > <img loading="lazy" src="https://images.novusbio.com/images/PAWR---PAR4-Antibody-Immunohistochemistry-Paraffin-NBP1-87338-img0013.jpg" alt="Immunohistochemistry-Paraffin PAWR / PAR4 Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/pawr-par4-antibody_nbp1-87338" title="Immunohistochemistry-Paraffin: PAWR / PAR4 Antibody [NBP1-87338] - Staining of human cerebellum shows moderate to strong cytoplasmic positivity in Purkinje cells." class="cust_viewed_8" > <img loading="lazy" src="https://images.novusbio.com/images/PAWR---PAR4-Antibody-Immunohistochemistry-Paraffin-NBP1-87338-img0014.jpg" alt="Immunohistochemistry-Paraffin PAWR / PAR4 Antibody" class="big_thumb" /> </a> </div></div><div id="ss_next_cav8" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-8" class="cycle-circle-pager"></div></div> <div class="ds_info"><div class="tiny"><strong>Species:</strong> Hu, Mu, Rt</div><div class="tiny"><strong>Applications:</strong> ICC/IF, IHC, IHC-P, WB</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NBP1-85047"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/tight-junction-protein-1-antibody_nbp1-85047" style="overflow: hidden; width: 280px;" title="Tight Junction Protein 1 Antibody">Tight Junction<br> Protein 1 Antibody</a>    </div><div class="sub-header">NBP1-85047</div> <div class="images_customers"><div id="ss_prev_cav9" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-9" data-cycle-prev="#ss_prev_cav9" data-cycle-next="#ss_next_cav9" data-cycle-slides="div.big_thumb_cust_container" data-index=9 ><div class="big_thumb_cust_container"> <a href="/products/tight-junction-protein-1-antibody_nbp1-85047" title="Immunocytochemistry/Immunofluorescence: Tight Junction Protein 1 Antibody [NBP1-85047] - Staining of Pig trabecular meshwork cells. ICC/IF image submitted by a verified customer review." class="cust_viewed_9" > <img loading="lazy" src="https://images.novusbio.com/images/Tight-Junction-Protein-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85047-img0013.jpg" alt="Immunocytochemistry/ Immunofluorescence Tight Junction Protein 1 Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/tight-junction-protein-1-antibody_nbp1-85047" title="Immunocytochemistry/Immunofluorescence: Tight Junction Protein 1 Antibody [NBP1-85047] - Staining of human cell line U-2 OS shows localization to cytosol &amp; cell junctions. Antibody staining is shown in green." class="cust_viewed_9" > <img loading="lazy" src="https://images.novusbio.com/images/Tight-Junction-Protein-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85047-img0008.jpg" alt="Immunocytochemistry/ Immunofluorescence Tight Junction Protein 1 Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/tight-junction-protein-1-antibody_nbp1-85047" title="Immunohistochemistry-Paraffin: Tight Junction Protein 1 Antibody [NBP1-85047] - Staining of human kidney shows strong membranous positivity in cells in glomeruli." class="cust_viewed_9" > <img loading="lazy" src="https://images.novusbio.com/images/Tight-Junction-Protein-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85047-img0014.jpg" alt="Immunohistochemistry-Paraffin Tight Junction Protein 1 Antibody" class="big_thumb" /> </a> </div></div><div id="ss_next_cav9" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-9" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/tight-junction-protein-1-antibody_nbp1-85047#ReviewsSection"> <div class="sprite_5Stars"></div> (5 Reviews</a>) </div> <div class="tiny"> <a href="/products/tight-junction-protein-1-antibody_nbp1-85047#PublicationSection"> <div class="sprite_pubBook"></div> (20 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu, Mu, Po, Rt</div><div class="tiny"><strong>Applications:</strong> ICC/IF, IHC, IHC-P, Simple Western, WB</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NBP2-67895"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/cdc42-antibody-jj086-04_nbp2-67895" style="overflow: hidden; width: 280px;" title="CDC42 Antibody (JJ086-04)">CDC42 Antibody<br> (JJ086-04)</a>    </div><div class="sub-header">NBP2-67895</div> <div class="images_customers"><div id="ss_prev_cav10" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-10" data-cycle-prev="#ss_prev_cav10" data-cycle-next="#ss_next_cav10" data-cycle-slides="div.big_thumb_cust_container" data-index=10 ><div class="big_thumb_cust_container"> <a href="/products/cdc42-antibody-jj086-04_nbp2-67895" title="Flow Cytometry: CDC42 Antibody (JJ086-04) [NBP2-67895] - Analysis of Hela cells with CDC42 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody." class="cust_viewed_10" > <img loading="lazy" src="https://images.novusbio.com/images/CDC42-Antibody-JJ086-04-Flow-Cytometry-NBP2-67895-img0001.jpg" alt="Flow Cytometry CDC42 Antibody (JJ086-04)" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/cdc42-antibody-jj086-04_nbp2-67895" title="Immunohistochemistry-Paraffin: CDC42 Antibody (JJ086-04) [NBP2-67895] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-CDC42 antibody. Counter stained with hematoxylin." class="cust_viewed_10" > <img loading="lazy" src="https://images.novusbio.com/images/CDC42-Antibody-JJ086-04-Immunohistochemistry-Paraffin-NBP2-67895-img0002.jpg" alt="Immunohistochemistry-Paraffin CDC42 Antibody (JJ086-04)" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/cdc42-antibody-jj086-04_nbp2-67895" title="Immunohistochemistry-Paraffin: CDC42 Antibody (JJ086-04) [NBP2-67895] - Analysis of paraffin-embedded human pancreas tissue using anti-CDC42 antibody. Counter stained with hematoxylin." class="cust_viewed_10" > <img loading="lazy" src="https://images.novusbio.com/images/CDC42-Antibody-JJ086-04-Immunohistochemistry-Paraffin-NBP2-67895-img0003.jpg" alt="Immunohistochemistry-Paraffin CDC42 Antibody (JJ086-04)" class="big_thumb" /> </a> </div></div><div id="ss_next_cav10" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-10" class="cycle-circle-pager"></div></div> <div class="ds_info"><div class="tiny"><strong>Species:</strong> Hu, Mu, Rt</div><div class="tiny"><strong>Applications:</strong> Flow, IHC, IHC-P, IP, WB</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NBP1-53125"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/gpsm2-antibody_nbp1-53125" style="overflow: hidden; width: 280px;" title="GPSM2 Antibody">GPSM2 Antibody</a>    </div><div class="sub-header">NBP1-53125</div> <div class="images_customers"><div id="ss_prev_cav11" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-11" data-cycle-prev="#ss_prev_cav11" data-cycle-next="#ss_next_cav11" data-cycle-slides="div.big_thumb_cust_container" data-index=11 ><div class="big_thumb_cust_container"> <a href="/products/gpsm2-antibody_nbp1-53125" title="Immunohistochemistry: GPSM2 Antibody [NBP1-53125] - Paraffin Embedded Tissue: Human Liver Antibody Concentration: 5 ug/ml" class="cust_viewed_11" > <img loading="lazy" src="https://images.novusbio.com/images/GPSM2-Antibody-Immunohistochemistry-NBP1-53125-img0011.jpg" alt="Immunohistochemistry GPSM2 Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/gpsm2-antibody_nbp1-53125" title="Western Blot: GPSM2 Antibody [NBP1-53125] - Sample Tissue: Human Lung Tumor Antibody Dilution: 1.0 ug/ml" class="cust_viewed_11" > <img loading="lazy" src="https://images.novusbio.com/images/GPSM2-Antibody-Western-Blot-NBP1-53125-img0012.jpg" alt="Western Blot GPSM2 Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/gpsm2-antibody_nbp1-53125" title="Immunocytochemistry/Immunofluorescence: GPSM2 Antibody [NBP1-53125] - Human cell lines." class="cust_viewed_11" > <img loading="lazy" src="https://images.novusbio.com/images/GPSM2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-53125-img0009.jpg" alt="Immunocytochemistry/ Immunofluorescence GPSM2 Antibody" class="big_thumb" /> </a> </div></div><div id="ss_next_cav11" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-11" class="cycle-circle-pager"></div></div> <div class="ds_info"><div class="tiny"><strong>Species:</strong> Hu</div><div class="tiny"><strong>Applications:</strong> ICC/IF, IHC, IHC-P, WB</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NBP2-67232"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/tyrosinase-antibody-ja52-11_nbp2-67232" style="overflow: hidden; width: 280px;" title="Tyrosinase Antibody (JA52-11)">Tyrosinase<br> Antibody (JA52-11)</a>    </div><div class="sub-header">NBP2-67232</div> <div class="images_customers"><div id="ss_prev_cav12" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-12" data-cycle-prev="#ss_prev_cav12" data-cycle-next="#ss_next_cav12" data-cycle-slides="div.big_thumb_cust_container" data-index=12 ><div class="big_thumb_cust_container"> <a href="/products/tyrosinase-antibody-ja52-11_nbp2-67232" title="Immunocytochemistry/Immunofluorescence: Tyrosinase Antibody (JA52-11) [NBP2-67232] - Staining Tyrosinase in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS." class="cust_viewed_12" > <img loading="lazy" src="https://images.novusbio.com/images/Tyrosinase-Antibody-JA52-11-Immunocytochemistry-Immunofluorescence-NBP2-67232-img0001.jpg" alt="Immunocytochemistry/ Immunofluorescence Tyrosinase Antibody (JA52-11)" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/tyrosinase-antibody-ja52-11_nbp2-67232" title="Immunocytochemistry/Immunofluorescence: Tyrosinase Antibody (JA52-11) [NBP2-67232] - Staining Tyrosinase in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS." class="cust_viewed_12" > <img loading="lazy" src="https://images.novusbio.com/images/Tyrosinase-Antibody-JA52-11-Immunocytochemistry-Immunofluorescence-NBP2-67232-img0002.jpg" alt="Immunocytochemistry/ Immunofluorescence Tyrosinase Antibody (JA52-11)" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/tyrosinase-antibody-ja52-11_nbp2-67232" title="Immunohistochemistry-Paraffin: Tyrosinase Antibody (JA52-11) [NBP2-67232] - Analysis of paraffin-embedded human liver tissue using anti-Tyrosinase antibody. Counter stained with hematoxylin." class="cust_viewed_12" > <img loading="lazy" src="https://images.novusbio.com/images/Tyrosinase-Antibody-JA52-11-Immunohistochemistry-Paraffin-NBP2-67232-img0003.jpg" alt="Immunohistochemistry-Paraffin Tyrosinase Antibody (JA52-11)" class="big_thumb" /> </a> </div></div><div id="ss_next_cav12" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-12" class="cycle-circle-pager"></div></div> <div class="ds_info"><div class="tiny"><strong>Species:</strong> Hu, Mu</div><div class="tiny"><strong>Applications:</strong> ICC/IF, IHC, IHC-P, WB</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="MAB3949"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/par2-antibody-344222_mab3949" style="overflow: hidden; width: 280px;" title="PAR2 Antibody (344222) [Unconjugated]">PAR2 Antibody<br> (344222) [Unconjugate</a>    </div><div class="sub-header">MAB3949</div> <div class="images_customers"><div id="ss_prev_cav13" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-13" data-cycle-prev="#ss_prev_cav13" data-cycle-next="#ss_next_cav13" data-cycle-slides="div.big_thumb_cust_container" data-index=13 ><div class="big_thumb_cust_container"> <a href="/products/par2-antibody-344222_mab3949" title="HT-29 human colon adenocarcinoma cell line was stained with Mouse Anti-Human PAR2 Monoclonal Antibody (Catalog # MAB3949, filled histogram) or isotype control antibody (MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (F0102B)." class="cust_viewed_13" > <img loading="lazy" src="https://images.novusbio.com/images2/PAR2_MAB3949_Flow_Cytometry_8427.jpg" alt="Flow Cytometry PAR2 Antibody (344222) [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/par2-antibody-344222_mab3949" title="PC-3 cells were stained with Mouse Anti-Human PAR2 Monoclonal Antibody (Catalog # MAB3949, filled histogram) or isotype control antibody (Catalog&amp;#160;# MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog&amp;#160;# F0102B). View our protocol for Staining Membrane-associated Proteins. " class="cust_viewed_13" > <img loading="lazy" src="https://images.novusbio.com/images/antibody/mab3949_human-par2-mab-clone-344222-flow-cytometry-512024112636..jpg" alt="Flow Cytometry PAR2 Antibody (344222) [Unconjugated]" class="big_thumb" /> </a> </div></div><div id="ss_next_cav13" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-13" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/par2-antibody-344222_mab3949#PublicationSection"> <div class="sprite_pubBook"></div> (2 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu</div><div class="tiny"><strong>Applications:</strong> CyTOF-ready, Flow</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NB500-178"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/numb-antibody_nb500-178" style="overflow: hidden; width: 280px;" title="Numb Antibody">Numb Antibody</a>    </div><div class="sub-header">NB500-178</div> <div class="images_customers"><div id="ss_prev_cav14" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-14" data-cycle-prev="#ss_prev_cav14" data-cycle-next="#ss_next_cav14" data-cycle-slides="div.big_thumb_cust_container" data-index=14 ><div class="big_thumb_cust_container"> <a href="/products/numb-antibody_nb500-178" title="Western Blot: NUMB Antibody [NB500-178] - Detection of NUMB isoforms 1 and 2 in A431 whole cell lysate (20 ug) using 0.5 ug/ml of NB500-178. ECL detection: 30 seconds." class="cust_viewed_14" > <img loading="lazy" src="https://images.novusbio.com/images/NUMB-Antibody-Western-Blot-NB500-178-img0005.jpg" alt="Western Blot Numb Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/numb-antibody_nb500-178" title="Immunocytochemistry/Immunofluorescence: NUMB Antibody [NB500-178] - The NUMB antibody was tested in HepG2 cells at a 1:500 dilution against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue), respectively." class="cust_viewed_14" > <img loading="lazy" src="https://images.novusbio.com/images/NUMB-Antibody-Immunocytochemistry-Immunofluorescence-NB500-178-img0004.jpg" alt="Immunocytochemistry/ Immunofluorescence Numb Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/numb-antibody_nb500-178" title="Immunocytochemistry/Immunofluorescence: Numb Antibody [NB500-178] - HepG2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with Numb Antibody (NB500-178) at 1ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective." class="cust_viewed_14" > <img loading="lazy" src="https://images.novusbio.com/images/Numb-Antibody-Immunocytochemistry-Immunofluorescence-NB500-178-img0007.jpg" alt="Immunocytochemistry/ Immunofluorescence Numb Antibody" class="big_thumb" /> </a> </div></div><div id="ss_next_cav14" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-14" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/numb-antibody_nb500-178#PublicationSection"> <div class="sprite_pubBook"></div> (7 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Ch, Hu, Mu</div><div class="tiny"><strong>Applications:</strong> ICC/IF, Simple Western, WB</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="DAGR00"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/human-agrp-quantikine-elisa-kit_dagr00" style="overflow: hidden; width: 280px;" title="AgRP/ART [HRP]">AgRP/ART [HRP]</a>    </div><div class="sub-header">DAGR00</div> <div class="images_customers"><div id="ss_prev_cav15" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-15" data-cycle-prev="#ss_prev_cav15" data-cycle-next="#ss_next_cav15" data-cycle-slides="div.big_thumb_cust_container" data-index=15 ><div class="big_thumb_cust_container"> <a href="/products/human-agrp-quantikine-elisa-kit_dagr00" title="" class="cust_viewed_15" > <img loading="lazy" src="https://images.novusbio.com/images2/DATA_AgRP_DAGR00_ELISA_569.jpg" alt="N/A AgRP/ART [HRP]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/human-agrp-quantikine-elisa-kit_dagr00" title="" class="cust_viewed_15" > <img loading="lazy" src="https://images.novusbio.com/images2/DATA_AgRP_DAGR00_ELISA_570.jpg" alt="N/A AgRP/ART [HRP]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/human-agrp-quantikine-elisa-kit_dagr00" title="" class="cust_viewed_15" > <img loading="lazy" src="https://images.novusbio.com/images2/AgRP_DAGR00_ELISA_27.jpg" alt="N/A AgRP/ART [HRP]" class="big_thumb" /> </a> </div></div><div id="ss_next_cav15" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-15" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/human-agrp-quantikine-elisa-kit_dagr00#PublicationSection"> <div class="sprite_pubBook"></div> (3 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu</div><div class="tiny"><strong>Applications:</strong> ELISA</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="H00055065-P01"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/gpr172b-recombinant-protein_h00055065-p01" style="overflow: hidden; width: 280px;" title="Recombinant Human GPR172B GST (N-Term) Protein">Recombinant Human<br> GPR172B GST (N-Term) ...</a>    </div><div class="sub-header">H00055065-P01</div> <div class="images_customers"><div class="one_image_l"></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-16" data-cycle-prev="#ss_prev_cav16" data-cycle-next="#ss_next_cav16" data-cycle-slides="div.big_thumb_cust_container" data-index=16 ><div class="big_thumb_cust_container"> <a href="/products/gpr172b-recombinant-protein_h00055065-p01" title="12.5% SDS-PAGE Stained with Coomassie Blue." class="cust_viewed_16" > <img loading="lazy" src="https://images.novusbio.com/images/qc_test-H00055065-P01-1.jpg" alt="Other Recombinant Human GPR172B GST (N-Term) Protein" class="big_thumb" /> </a> </div></div><div class="one_image"></div><div class="one_image_11"></div></div> <div class="ds_info"><div class="tiny"><strong>Species:</strong> Hu</div><div class="tiny"><strong>Applications:</strong> ELISA, AP, PA, WB</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NBP1-88861"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/pard3-par3-antibody_nbp1-88861" style="overflow: hidden; width: 280px;" title="PARD3/Par3 Antibody">PARD3/Par3 Antibody</a>    </div><div class="sub-header">NBP1-88861</div> <div class="images_customers"><div class="one_image_l"></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-17" data-cycle-prev="#ss_prev_cav17" data-cycle-next="#ss_next_cav17" data-cycle-slides="div.big_thumb_cust_container" data-index=17 ><div class="big_thumb_cust_container"> <a href="/products/pard3-par3-antibody_nbp1-88861" title="PARD3/Par3 Antibody" class="cust_viewed_17"> <img loading="lazy" src="/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png" alt="PARD3/Par3 Antibody" title="PARD3/Par3 Antibody" class=" big_thumb" /> </a> </div></div><div class="one_image"></div><div class="one_image_11"></div></div> <div class="ds_info"><div class="tiny"><strong>Species:</strong> Hu, Mu</div><div class="tiny"><strong>Applications:</strong> WB, Simple Western, ICC/IF, IHC, IP</div> </div> </div> </div></div> </div><div id="cav_next"><div class="sprite_rightArrow"></div></div> <div class="clear"></div></div> <div class="ds_tab_content equalbottom" id="content_reviews-publications"> <a id="reviews-publications"></a><a id="PublicationSection"></a><h2 class="greyHead2">Publications for PARD3/Par3 Antibody (NBP1-88861)(10)</h2><div class="ds_info"> <div>We have publications tested in 2 confirmed species: Human, Mouse.<br /><br />We have publications tested in 5 applications: ICC/IF, IF/IHC, IHC-Fr, IHC-P, IP.<br /><br /><div class="align-right"> <button class="greybutton btn_width" name="novusreview" onclick="window.location='/publications'"; >Submit a Publication</button><br /> </div> </div><div> <div class="filterParent"> <div class="publications_filterList" id="pubapp"> <div class="blueHead">Filter By Application</div><div class="filter_line"> <input type="checkbox" value="application" class="publication_filterCheckbox" data-filter="ICC/IF"> <div style="display:inline;">ICC/IF</div> (3)</div><div class="filter_line"> <input type="checkbox" value="application" class="publication_filterCheckbox" data-filter="IF/IHC"> <div style="display:inline;">IF/IHC</div> (2)</div><div class="filter_line"> <input type="checkbox" value="application" class="publication_filterCheckbox" data-filter="IHC-Fr"> <div style="display:inline;">IHC-Fr</div> (1)</div><div class="filter_line"> <input type="checkbox" value="application" class="publication_filterCheckbox" data-filter="IHC-P"> <div style="display:inline;">IHC-P</div> (1)</div><div class="filter_line"> <input type="checkbox" value="application" class="publication_filterCheckbox" data-filter="IP"> <div style="display:inline;">IP</div> (1)</div><div class="filter_clear" data-type="publication" data-filter="application">All Applications</div></div> <div class="publications_filterList" id="pubspec"> <div class="blueHead">Filter By Species</div><div class="filter_line"> <input type="checkbox" value="species" class="publication_filterCheckbox" data-filter="Human"> <div style="display:inline;">Human</div> (1)</div><div class="filter_line"> <input type="checkbox" value="species" class="publication_filterCheckbox" data-filter="Mouse"> <div style="display:inline;">Mouse</div> (4)</div><div class="filter_clear" data-type="publication" data-filter="species">All Species</div></div> </div></div> <div class="clear"></div> <div id="publications_list">Showing Publications 1 - <span id="appcnt">10</span> of 10.<table class="wide lightPad tablesorter" id="publications_list_table"> <thead> <tr> <th>Publications using NBP1-88861</th> <th>Applications</th> <th>Species</th> </tr> </thead> <tbody><tr class="lined revfil firstten" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/37493885" target="_blank" rel="nofollow"> Wang J, Cheng X, Mei X et al. The effect of Par3 on the cellular junctions and biological functions of odontoblast-lineage cells Odontology 2023-07-26 [PMID: 37493885] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil firstten" data-application="ICC/IF" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/35316074" target="_blank" rel="nofollow"> Das A, Adhikary S, Chowdhury AR Et al. Leveraging Substrate Stiffness to Promote Stem Cell Asymmetric Division via Mechanotransduction-Polarity Protein Axis and Its Bayesian Regression Analysis Rejuvenation Res 2022-03-22 [PMID: 35316074] (ICC/IF)<br /><br />Details:<br />Citation using the Texas Red version of this antibody. </a></td> <td valign="top">ICC/IF</td> <td valign="top"></td> </tr><tr class="lined revfil firstten" data-application="IHC-Fr" data-species="Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/34706238" target="_blank" rel="nofollow"> Heinrich A, Bhandary B, Potter SJ Et al. Cdc42 activity in Sertoli cells is essential for maintenance of spermatogenesis Cell reports 2021-10-26 [PMID: 34706238] (IHC-Fr, Mouse) </a></td> <td valign="top">IHC-Fr</td> <td valign="top">Mouse</td> </tr><tr class="lined revfil firstten" data-application="IP" data-species="Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/34158849" target="_blank" rel="nofollow"> Zhou Y, Ji H, Xu Q et al. Congenital biliary atresia is correlated with disrupted cell junctions and polarity caused by Cdc42 insufficiency in the liver Theranostics 2021-05-24 [PMID: 34158849] (IP, Mouse) </a></td> <td valign="top">IP</td> <td valign="top">Mouse</td> </tr><tr class="lined revfil firstten" data-application="IHC-P" data-species="Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/33548596" target="_blank" rel="nofollow"> Engevik AC, Krystofiak ES, Kaji I et al. Recruitment of Polarity Complexes and Tight Junction Proteins to the Site of Apical Bulk Endocytosis Cellular and molecular gastroenterology and hepatology 2021-02-03 [PMID: 33548596] (IHC-P, Mouse) </a></td> <td valign="top">IHC-P</td> <td valign="top">Mouse</td> </tr><tr class="lined revfil firstten" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/33172988" target="_blank" rel="nofollow"> Ling J, Sckaff M, Tiwari M et al. RAS-mediated suppression of PAR3 and its effects on SCC initiation and tissue architecture occur independently of hyperplasia Journal of Cell Science 2020-05-18 [PMID: 33172988] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil firstten" data-application="IF/IHC" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/32294451" target="_blank" rel="nofollow"> Heinrich A, Potter SJ, Guo L et al. Distinct Roles for Rac1 in Sertoli Cell Function during Testicular Development and Spermatogenesis Cell Rep 2020-04-14 [PMID: 32294451] (IF/IHC, Human) </a></td> <td valign="top">IF/IHC</td> <td valign="top">Human</td> </tr><tr class="lined revfil firstten" data-application="ICC/IF" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/28923978" target="_blank" rel="nofollow"> Moreno-Fortuny A, Bragg L, Cossu G, Roostalu U. MCAM contributes to the establishment of cell autonomous polarity in myogenic and chondrogenic differentiation. Biol Open. 2017-09-18 [PMID: 28923978] (ICC/IF) </a></td> <td valign="top">ICC/IF</td> <td valign="top"></td> </tr><tr class="lined revfil firstten" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/28350047" target="_blank" rel="nofollow"> Tuccilli C, Baldini E, Arlot-Bonnemains Y et al. Expression and prognostic value of the cell polarity PAR complex members in thyroid cancer. Int J Oncol 2017-03-08 [PMID: 28350047] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil firstten" data-application="IF/IHC, ICC/IF" data-species="Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/24164893" target="_blank" rel="nofollow"> Whiteman EL, Fan S, Harder JL et al. Crumbs3 is Essential for Proper Epithelial Development and Viability. Mol Cell Biol. 2013-10-28 [PMID: 24164893] (IF/IHC, ICC/IF, Mouse) </a></td> <td valign="top">IF/IHC, ICC/IF</td> <td valign="top">Mouse</td> </tr></tbody> </table> </div> </div><a id="ReviewsSection"></a> <h2 class="greyHead2">Reviews for <span>PARD3/Par3 Antibody (NBP1-88861)</span> (2) <span style="display:none;">4</span><span style="display:none;">2</span></h2> <div class="ds_info"> <div class="bottom15"> <strong>Average Rating: 4</strong> <div class="dsSprite_stars4"></div> (Based on 2 reviews) </div> <div><div class="ds_info">We have 2 reviews tested in 2 species: Human, Mouse.</div> <div class="align-right"> <button class="greybutton btn_width" id="novusreview" name="novusreview" onclick="window.location='/reviews/NBP1-88861'"; >Submit a Review</button><br /> </div> </div> <div class="blueHead"><strong>Reviews using NBP1-88861:</strong></div> <div class="filterParent"><div class="reviews_filterList"> <div class="blueHead">Filter by Applications</div><div class="filter_line"> 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<thead> <tr> <th>Images</th> <th>Ratings</th> <th>Applications</th> <th>Species</th> <th>Date</th> <th>Details</th> </tr> </thead> <tbody><tr class="top-lined revfil" data-rating="Very Good" data-application="IF" data-species="Human"> <td><a href="https://images.novusbio.com/reviews/Immunofluorescence_PARD3-Antibody-(NBP1-88861)-(01-ml)_NBP1-88861_7161.Jpg" title="8505C cells (anaplastic thyroid cancer cell line)" class="colorbox" title="Immunofluorescence" > <img loading="lazy" src="https://images.novusbio.com/reviews/Immunofluorescence_PARD3-Antibody-(NBP1-88861)-(01-ml)_NBP1-88861_7161.Jpg" class="reviews_imageBorder" width="75" alt="Immunofluorescence PARD3/Par3 NBP1-88861" /> <br/><span class="enlarge" href="#"><div class="dsSprite_magGlass"></div> Enlarge</span></a><meta content="04/29/2014" /> </td> <td> <span style="display:none;">4</span> <div class="dsSprite_stars4" content="4"></div> <div class="revby">reviewed by:<br />enke baldini</div> </td> <td title="Immunofluorescence">IF</td> <td>Human</td> <td>04/29/2014</td> <td align="left"> <div class="dsSprite_expandReviews"><div class="expandReviewData">View</div></div> <div class="reviews_showMoreContent"> <div class="reviews_showMoreContentInner"><h2 class="greyHead">Summary</h2> <table class="even wide top"><tr><td class="text_bold">Application</td><td>Immunofluorescence</td></tr><tr><td class="text_bold">Sample Tested</td><td>cell line derived from anaplastic thyroic cancer</td></tr><tr><td class="text_bold">Species</td><td>Human</td></tr><tr><td class="text_bold">Lot</td><td>A57864</td></tr></table> </div> </div> </td> </tr><tr class="top-lined revfil" data-rating="Very Good" data-application="ICC" data-species="Mouse"> <td><a href="https://images.novusbio.com/reviews/review_image_22976_5631_1383782858.jpg" title="Immunocytochemistry image for NBP1-88861" class="colorbox" title="Immunocytochemistry" > <img loading="lazy" src="https://images.novusbio.com/reviews/review_image_22976_5631_1383782858.jpg" class="reviews_imageBorder" width="75" alt="Immunocytochemistry PARD3/Par3 NBP1-88861" /> <br/><span class="enlarge" href="#"><div class="dsSprite_magGlassBlue"></div> Enlarge</span></a><meta content="11/07/2013" /> </td> <td> <span style="display:none;">4</span> <div class="dsSprite_stars4" content="4"></div> <div class="revby">reviewed by:<br />Andrew Cosgrove</div> </td> <td title="Immunocytochemistry">ICC</td> <td>Mouse</td> <td>11/07/2013</td> <td align="left"> <div class="dsSprite_expandReviews"><div class="expandReviewData">View</div></div> <div class="reviews_showMoreContent"> <div class="reviews_showMoreContentInner"><h2 class="greyHead">Summary</h2> <table class="even wide top"><tr><td class="text_bold">Application</td><td>Immunocytochemistry</td></tr><tr><td class="text_bold">Sample Tested</td><td>Mouse trophoblast stem cells</td></tr><tr><td class="text_bold">Species</td><td>Mouse</td></tr></table> </div> </div> </td> </tr></tbody> </table> </div><br /> </div> <div class="clear"></div></div> <div class="ds_tab_content equalbottom" id="content_protocols-faqs"> <a id="protocols-faqs"></a><h2 class="greyHead2">Product General Protocols</h2> <a id="protocols"></a> <div class="ds_info">Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars. <ul><li><a href="/support/support-by-application/immunocytochemistry/protocol.html">Immunocytochemistry/ Immunofluorescence</a></li><li><a href="/support/support-by-application/immunohistochemistry-paraffin/protocol.html">Immunohistochemistry-Paraffin</a></li><li><a href="/support/support-by-application/immunoprecipitation/protocol.html">Immunoprecipitation</a></li><li><a href="/support/support-by-application/western-blot/protocol.html">Western Blot</a></li><li><a href="/support/support-by-application.html">View all Protocols, Troubleshooting, Illustrated assays and Webinars.</a></li> </ul></div><h2 class="greyHead2 ds_info">Video Protocols</h2> <a id="videos"></a> <div class="ds_info"><div class="movie-cell"> <span class="movie-title">WB Video Protocol</span><br/> <meta content ="https://www.youtube.com/watch?v=uFu8aie4QFI" /> <a href="https://www.youtube.com/embed/uFu8aie4QFI?rel=0&wmode=transparent" class="youtube"> <div class="reduce sprite_WBvideo"></div> </a> </div><div class="movie-cell"> <span class="movie-title">ICC/IF Video Protocol</span><br/> <meta content ="https://www.youtube.com/watch?v=5AcrhhHmQZs" /> <a href="https://www.youtube.com/embed/5AcrhhHmQZs?rel=0&wmode=transparent" class="youtube"> <div class="reduce sprite_ICC-IFvideo"></div> </a> </div></div> <div class="clear"></div><h2 class="greyHead2">FAQs for PARD3/Par3 Antibody (NBP1-88861) (0)</h2> <a id="faqs"></a> <div class="ds_info">There are no specific FAQs related to this product. Read our general <a href="/support/faqs.html">customer & technical service FAQs.</a> </div> <div class="clear"></div></div> <div class="ds_tab_content equalbottom" id="content_supportresearch"> <a id="supportresearch"></a><table cellpadding="0" cellspacing="0"><tr><td valign="top" class="width50"> <h2 class="greyHead2">Secondary Antibodies</h2> <a id="secondaryantibodies"></a> <div class="ds_info"> <ul class="information-list"><li><a href="/product-type/secondary-antibodies?species=Rabbit&facet_conjugate=HRP">Anti-Rabbit HRP Labeled Antibodies</a></li><li><a href="/product-type/secondary-antibodies?species=Rabbit&facet_conjugate=FITC">Anti-Rabbit FITC Labeled Antibodies</a></li><li><a href="/product-type/secondary-antibodies?species=Rabbit&facet_conjugate=Biotin">Anti-Rabbit Biotin Labeled Antibodies</a></li><li> </li> <li><a href="/product-type/secondary-antibodies#fq=species%3A%22Rabbit%22">View all of our anti-Rabbit Secondary Antibodies</a></li> </ul> </div></td><td width="5"> </td><td valign="top"> <h2 class="greyHead2" >Isotype Controls</h2> <a id="isotypecontrols"></a> <div class="ds_info"> <ul class="ds_noDotList"><li><a href="/product-type/isotype-controls#fq=species%3A%22Rabbit%22">Rabbit Isotype Controls</a></li><li> </li> <li><a href="/product-type/isotype-controls">View all of our Isotype Control Products</a></li> </div></td></tr></table><h2 class="greyHead2">Additional PARD3/Par3 Products</h2> <a id="addtlalphaprods"></a> <div class="ds_info"> <div> <span style="display:none;">Array NBP1-88861</span> <ul class="ds_noDotList"><li> <a href="/primary-antibodies/pard3-par3">PARD3/Par3 Antibodies</a></li><li> <a href="/proteins/pard3-par3">PARD3/Par3 Proteins</a></li></ul> </div> </div><table cellpadding="0" cellspacing="0" class="width100"><tr></tr></table><table cellpadding="0" cellspacing="0" class="width100"><tr><td valign="top"><h2 class="greyHead2">Research Areas for PARD3/Par3 Antibody (NBP1-88861)</h2> <a id="research_areas"></a> <div class="ds_info">Find related products by research area.</div> <div class="ds_info"> <ul class="ds_noDotList"><li class="left width"><a href="/research-areas/cell-biology/cell-cycle-and-replication">Cell Cycle and Replication</a></li></ul> </div></td></tr></table><h2 class="greyHead2">Blogs on PARD3/Par3</h2> <a id="blogsect"></a> <div>There are no specific blogs for PARD3/Par3, but you can <a href="/antibody-news/">read our latest blog posts.</div> <div class="clear"></div></div> </div></div><div class="grid_6"><div class="ds_blue_border marTop26 equalbottom"><div class="datasheet-ads-container" ><a href="/products/mfluor-violet-fluorescent-conjugated-antibodies" target="_parent"><img loading="lazy" src="https://images.novusbio.com/design/mfluor-violet-conjugated-antibodies-banner-final-v5-349x119.jpg" alt="mFluor Violet Conjugated Antibodies" title="mFluor Violet Conjugated Antibodies" class="datasheet-adzone" loading="lazy"></a></div><a id="jump_recommended"></a> <h2 class="greyHead2">Customers Who Bought This Also Bought</h2> <div class="text_center"><div class="images2"><div id="ss_prev_sidebar" class="cycle-prev ds_rp_prev"><div class="pager_left_on"></div></div><div id="ss_next_sidebar" class="cycle-next ds_rp_next"><div class="pager_right_on"></div></div><div class="cycle-slideshow" data-cycle-fx="scrollHorz" data-cycle-timeout="0" data-cycle-prev="#ss_prev_sidebar" data-cycle-next="#ss_next_sidebar" data-cycle-slides="div.big_thumb_container" id="ds_customer_who_bought" ><div class="big_thumb_container" data-catnums="HAF008"> <div class="img_container"> <a href="/products/igg-antibody_haf008" title="Western blot shows detection of Rabbit Anti-Human/Mouse/Rat HSP60 Polyclonal Antibody (AF1800) using a 1:1000 dilution of HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="cust_bought" > <img loading="lazy" src="https://images.novusbio.com/images2/IgG_HAF008_Western_Blot_19080.jpg" title="Western blot shows detection of Rabbit Anti-Human/Mouse/Rat HSP60 Polyclonal Antibody (AF1800) using a 1:1000 dilution of HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows detection of Rabbit Anti-Human/Mouse/Rat HSP60 Polyclonal Antibody (AF1800) using a 1:1000 dilution of HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" /> </a> </div> <div class="caption_container"><a href="/products/igg-antibody_haf008">Goat anti-Rabbit IgG Secondary Antibody [HRP]</a> <br />HAF008</div> <div style="text-align: right"><button class="greybutton" id="novusviewdatasheet" name="novusviewdatasheet" onclick="window.location='/products/igg-antibody_haf008';" >View Datasheet</button> </div> </div><div class="big_thumb_container" data-catnums="NB7160"> <div class="img_container"> <a href="/products/igg-h-l-antibody_nb7160" title="Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242)." class="cust_bought" > <img loading="lazy" src="https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg" title="Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242)." alt="Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). 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Goat anti-rabbit FITC (Novus, 20302) was used as secondary." class="big_thumb" /> </a> </div> <div class="caption_container"><a href="/products/igg-isotype-control_nbp2-24891">Rabbit IgG Isotype Control</a> <br />NBP2-24891</div> <div style="text-align: right"><button class="greybutton" id="novusviewdatasheet" name="novusviewdatasheet" onclick="window.location='/products/igg-isotype-control_nbp2-24891';" >View Datasheet</button> </div> </div></div></div></div><div class="clear"></div><a id="jump_distributor"></a> <h2 class="greyHead2">Contact Information</h2> <div class="ds_info"> <div id="distributors"></div> </div> <div id="et_reload_trigger" style="display:none;">reload</div><h2 class="greyHead2">Product PDFs</h2><div class="silverHeadShort"> <span class="file"><a href="//aero.bio-techne.com/sds/pdf/nbp1-88861" target="_blank" class="sprite_dsPDF"><span class="pdfSmaller"> </span></a></span> <span class="download-file-text" ><a href="//aero.bio-techne.com/sds/pdf/nbp1-88861" target="_blank" class="datasheet-download sdsFile">SDS</a></span> </div><div class="silverHeadShort"> <span class="file"><a href="//www.novusbio.com/PDFs/NBP1-88861.pdf" target="_blank" class="sprite_dsPDF"><span class="pdfSmaller"> </span></a></span> <span class="download-file-text" ><a href="//www.novusbio.com/PDFs/NBP1-88861.pdf" target="_blank" class="datasheet-download">PDF Datasheet</a></span> </div><div class="silverHeadShort"> <span class="file"><a href="mailto:novus@novusbio.com?subject=Request%20CofA%20for%20NBP1-88861" class="sprite_newDocument"><span class="downloadBlank"> </span></a></span> <span class="download-file-text"><a href="mailto:nb-technical@bio-techne.com?subject=Request%20CofA%20for%20NBP1-88861&body=Specific%20catalog%20number(if multiple sizes):%0ALot%20Number:%0AAny%20additional%20products:">Request CofA</a></span> </div><h2 class="greyHead2">Calculators</h2> <div class="calc_wrapper"> <div class="dsSprite_calc"></div> <div class="fake_link" id="calc_reconstitution_link">Concentration Calculator</div> <div id="calc_reconstitution_wrapper" class="calc_function_wrapper"> <div class="calc_popup"> <div class="calc_close"> <div class="dsSprite_calc_close"></div> </div> <div class="calc_header">Concentration Calculator</div> <div id="reconstitution_calculator" class="calculator"> <div class="reconstitution_calculator_text" id="reconstitution_calculator_instructions"> <p>The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. 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</div> </div> <h2 class="greyHead2">Recent Reviews</h2><div class="review_summary"> <div class="review_summary_left"> <div class="review_large_number">4</div> <div class="dsSprite_stars4"></div> </div> <div class="review_summary_right"> <table> <tbody><tr><td><div class="dsSprite_emptyStar"></div><div class="review_summary_star_number">5</div></td><td style="position:relative;width:155px;"><div class="review_bar star5" style="width:0%;"></div><div class="review_bar_value">0</div></td></tr><tr><td><div class="dsSprite_emptyStar"></div><div class="review_summary_star_number">4</div></td><td style="position:relative;width:155px;"><div class="review_bar star4" style="width:100%;"></div><div class="review_bar_value">2</div></td></tr><tr><td><div class="dsSprite_emptyStar"></div><div class="review_summary_star_number">3</div></td><td style="position:relative;width:155px;"><div class="review_bar star3" style="width:0%;"></div><div class="review_bar_value">0</div></td></tr><tr><td><div 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/> <br/><span class="enlarge" href="#"><div class="dsSprite_magGlass"></div> Enlarge</span></a></div> <div class="review_summary_right"><table> <tbody> <tr> <td><div class="dsSprite_stars4" content="4"></div></td> <td>enke baldini<br />04/29/2014</td> </tr> <tr> <td class="review_summary_title">Application: </td> <td>IF<td> </tr> <tr> <td class="review_summary_title">Species: </td> <td>Human</td> </tr> </tbody> </table> <div class="text_right" style="margin-bottom:-8px;"><a href="#ReviewsSection">read this review</a></div></div></div> <hr /><div class="single_review"><div class="review_summary_image"><a href="//images.novusbio.com/reviews/review_image_22976_5631_1383782858.jpg" title="Immunocytochemistry image for NBP1-88861" class="colorbox" title="Immunocytochemistry" > <img loading="lazy" src="//images.novusbio.com/reviews/review_image_22976_5631_1383782858.jpg" class="reviews_imageBorder" width="75" alt="Immunocytochemistry PARD3/Par3 NBP1-88861" /> <br/><span class="enlarge" href="#"><div class="dsSprite_magGlass"></div> Enlarge</span></a></div> <div class="review_summary_right"><table> <tbody> <tr> <td><div class="dsSprite_stars4" content="4"></div></td> <td>Andrew Cosgrove<br />11/07/2013</td> </tr> <tr> <td class="review_summary_title">Application: </td> <td>ICC<td> </tr> <tr> <td class="review_summary_title">Species: </td> <td>Mouse</td> </tr> </tbody> </table> <div class="text_right" style="margin-bottom:-8px;"><a href="#ReviewsSection">read this review</a></div></div></div> <hr /><div class="align-right" style="margin-right:10px;"> <a href="#ReviewsSection"> <button class="greybutton btn_width">read all the reviews</button> </a> </div> </div><h2 class="greyHead2">Bioinformatics</h2> <div class="ds_info"> <a id="tab1bioinformatics"></a> <div> <table class="ds_list_right wide top"> <tr class="lined"> <td><strong>Gene Symbol</strong></td> <td><a href="/gene-name/PARD3">PARD3</a></td> </tr></table> </div> </div> </div> </div><div class="clear"></div> 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