mRNA Synthesis | GENEWIZ from Azenta

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Whole Genome Sequencing</a></li><li><a href='/en/Public/Services/Clinical-Services/Regulatory' style='color:#585F69;font-size:14px'>GLP-Compliant Overview</a></li><li><a href='/en/Public/Services/Clinical-Services/GLP-compliant-Confirmatory-Sequencing' style='color:#585F69;font-size:14px'>GLP Confirmatory Sequencing</a></li><li><a href='/en/Public/Services/Clinical-Services/GLP-compliant-SNP-Mutation-Analysis' style='color:#585F69;font-size:14px'>GLP SNP Mutation Analysis</a></li><li><a href='/en/Public/Services/Clinical-Services/GLP-compliant-Plasmid-Prep' style='color:#585F69;font-size:14px'>GLP Plasmid Prep</a></li><li><a href='/en/Public/Services/Clinical-Services/Nucleic-Acid-Extraction' style='color:#585F69;font-size:14px'>GLP Nucleic Acid Extraction</a></li><li><a href='/en/Public/Services/Clinical-Services/Biofluid-Processing-Overview' style='color:#585F69;font-size:14px'>Biofluid Processing</a></li><li><a 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industry-leading project completion time, a wide range of deliverables, and best-in-class quality.</p> <p>With the development of in vitro transcription (IVT) methods, messenger ribonucleotides (mRNA) synthesis has become an emerging class of gene therapy. Serving as the molecular bridge between DNA and proteins, mRNAs and their in vitro transcription technology enable the concept of nucleic acid-encoded proteins for research and therapeutic purposes. The transient nature of mRNA activity also offers an alternative approach to gene editing and gene therapy for delivering genetic information into cells.</p> <p>GENEWIZ Multiomics and Synthesis Solutions from Azenta Life Sciences equips researchers with a range of flexible and tailored solutions that save valuable time and accelerate innovation.</p> <p> </p> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://web.genewiz.com/gene-synth-inquiry?utm_source=custom-mrna-synthesis" target="_blank"><button>Contact an Expert</button></a> </div> <div class="oligo-content-box" id="oligo-content-box-right"> <iframe width="550" height="350" src="https://www.youtube.com/embed/3tI2QdXZxxU" title="Custom mRNA Production via Gene Synthesis and In Vitro Transcription" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture"></iframe> </div> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> .question { width: 1170px; display: inline-block; } .question p { width: 90%; text-align: left; } .question ul { width: 90%; text-align: left; } @media screen and (max-width: 1170px) { .question { width: auto; } } </style> <div class="question"> <h2>What are the steps of <span style="text-transform: none;">m</span>RNA synthesis?</h2> <p>Messenger RNA (mRNA) synthesis begins with codon optimization of this sequence for optimal transcription. The gene is synthesized, cloned into a vector, and verified by Sanger sequencing. The plasmid DNA is then amplified, target mRNA sequence linearized, and used as a template for in vitro transcription.</p> <p> </p> </div> </div> <script type="text/javascript" src="/assets/plugins/counter/jquery.counterup.min.js"></script> <script language="javascript" type="text/javascript"> jQuery(document).ready(function ($) { $(".row .category").find("img").addClass("img-responsive"); }); </script> <div class='Component-Frame pull-center Multi-column-TextBullet-Layout Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10'> <div class="container"> <div class="headline-center"> <h2>Custom <span style="text-transform: none;">m</span>RNA Synthesis Applications</h2> </div> <div class="headline-left"> </div> <div class="row category"> <div class="col-md-6 col-sm-6"> <div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> <strong>Therapeutic Development:</strong> Treat diseases of protein expression and cellular function without gene editing </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> <strong>Cancer Research:</strong> Express tumor antigens or chimeric antigen receptors (CARs) to investigate cancer treatment and response. </div> </div> </div> <div class="col-md-6 col-sm-6"> <div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> <strong>CRISPR/Gene Editing:</strong> Create targeting mRNA sequences for delivery of CRISPR/Cas9 and other gene editing platforms. </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> <strong>Antibody Development:</strong> Translate antibodies <em>in vivo</em> from encoded mRNA instead of from monoclonal production. </div> </div> </div> </div> </div> </div><div class="headline-center Rich-Text-Edit-Area"> <link href="https://fonts.googleapis.com/css?family=Roboto:300,400,500,700" rel="stylesheet" /> <style type="text/css"> .covid-banner { width: 1170px; height: auto; margin: 0 auto; background-image: url("/-/media/Canva_OMICS_Webinar5_WebBanFull_Medium_BACKGROUND.ashx"); background-size: cover; background-position: center; text-align: left; padding: 0px 0 0px 0; } .covid-content { text-align: center; } .covid-box h3 { color: #ffffff; text-transform: none; letter-spacing: 1px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; line-height: 110%; font-size: 45px; margin: 0; padding-bottom: 15px; padding-top: 25px; } .covid-box h4 { color: #ffffff; text-transform: none; letter-spacing: 1px; line-height: 25px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 20px; margin: 0; margin-bottom: 5px; } .covid-box p { color: #000000; text-transform: none; letter-spacing: 1px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 15px; margin: 0; margin-bottom: 5px; } .covid-box button { padding: 8px 19px; color: #826ec3; letter-spacing: 1px; background: #ffffff; border: 2px solid #ffffff; border-radius: 0px; transition: all .2s ease-in-out; font-size: 15px } .covid-box button:hover { cursor: pointer; transform: scale(1.1); } .covid-box button:focus { outline: 0; } @media only screen and (max-width: 1170px) { .covid-banner { width: 100%; height: auto; } .covid-content { width: 90%; margin: 0 auto; } } </style> <br /> <div class="covid-banner"> <div class="covid-content"> <div class="covid-box"> <br /> <h4>OMICS TECHNOLOGY SPOTLIGHT WEBINAR</h4> <h3>Engineering the Future: Unlocking the Potential of<br /> Functional RNAs in Biology, Medicine, and Innovation</h3> <h4>Thursday, July 25, 2024 | 11:00AM EDT</h4> <a href="https://hubs.ly/Q02FV0q90" target="_blank"> <br /> <button> SAVE MY SPOT</button> <br /> </a> <p> </p> </div> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <div class="data-container"> <div class="container"> <h2>How do you synthesize <span style="text-transform: none;">m</span>RNA?</h2> <p>Synthetic mRNA comes from a template nucleotide sequence of DNA. The process starts with <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://www.genewiz.com/Public/Services/Gene-Synthesis/codon-optimization" target="_blank">codon optimization</a> of this sequence for optimal transcription. The gene is synthesized, cloned into a vector, and verified by Sanger sequencing. The plasmid DNA is then amplified, target mRNA sequence linearized, and used as a template for <em>in vitro</em> transcription. </p> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <span id="mRNA-Transcription" class="anchor"></span> <style type="text/css"> .workflow-container { padding-top: 30px; background: #fffffff; } .workflow { text-align: left; width: 1170px; display: inline-block } .workflow-step { vertical-align: top; width: 19%; padding: 10px; margin-bottom: 20px; display: inline-block; } .workflow-step img { width: 120px; margin: 0 auto; margin-bottom: 20px; } .workflow-step h2 { width: 80%; text-align: center; } .workflow-step h3 { text-align: center; font-size: 18px; font-family: sans-serif; border-bottom: none; } .workflow-step p { width: 80%; margin: 0 auto; text-align: left; } .workflow-step p1 { width: 80%; margin: 0 auto; text-align: left; font-size:15px; } .workflow-icon { text-align: center; } @media only screen and (max-width: 1170px) { .workflow-container { width: auto; } .workflow { text-align: left; width: auto; } .workflow-step { width: 100%; } } </style> <div class="workflow-container"> <div class="workflow"> <h2 style="text-align: center; margin-bottom: 30px;"><span style="text-transform: none;">m</span>RNA TranScription Service</h2>   <div class="workflow-step"> <div class="workflow-icon"> <img alt="Clinical-Icon" src="/-/media/12035-D-mRNA_Construct-Design_V2.ashx?la=en&hash=D379A17338D3A9FC2405E11074AFB218E39AFE0B" /></div> <div class="workflow-icon"><strong> </strong> <p1><strong>1. Construct Design</strong></p1> <br /> <p><strong><br /> Codon Optimization:</strong> <br /> Maximize protein expression and experimental performance with <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://www.genewiz.com/Public/Services/Gene-Synthesis/Codon-Optimization/" target="_blank">Azenta's propriety codon optimization tool</a></p> </div> </div> <div class="workflow-step"> <div class="workflow-icon"> <img alt="Clinical-Icon" src="/-/media/12035-D-mRNA_DNA-Synthesis_V2.ashx?la=en&hash=CE51D9A1BCCF8416262240B8A158C8C3041FCE4D" /></div> <div class="workflow-icon"><strong> <p1>2. DNA Synthesis</p1> </strong> <p><strong> </strong></p> <p><strong> Oligo and Gene Synthesis:</strong> 99.9% reliability in project delivery rate from a team with over 20 years of <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://www.genewiz.com/Public/Services/Gene-Synthesis" target="_blank">gene synthesis experience</a></p> </div> </div> <div class="workflow-step"> <div class="workflow-icon"> <img alt="Antibody-Icon" src="/-/media/12035-D-mRNA_Cloning-and-Template-Plasmid-Prep_V2.ashx?la=en&hash=B70AC159DB0FF55AAC58425241A002A9C02004EE" /></div> <div class="workflow-icon"><strong> <p1>3. Cloning and Plasmid Prep</p1> </strong> <br /> <p><strong><br /> Template Plasmid:  <br /> </strong>Performed by our experienced molecular biologists to <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://www.genewiz.com/Public/Services/Gene-Synthesis/DNA-Cloning" target="_blank">quickly generate plasmid DNA</a> for IVT based on desired mRNA yield</p> </div> </div> <div class="workflow-step"> <div class="workflow-icon"> <img alt="Antibody-Icon" src="/-/media/12035-D-mRNA_In-Vitro-Transcription_V2.ashx?la=en&hash=C1393835B8D8112D032D7BB504E6F56BE5D2D531" /></div> <div class="workflow-icon"><strong> <p1>4. <em>In Vitro</em> Transcription</p1> </strong> <br /> <p><strong><br /> Scalable Transcription:</strong>    <br /> 100 ug to 10 mg quantities of mRNA available to grow with your research program</p> </div> </div> <div class="workflow-step"> <div class="workflow-icon"> <img alt="Antibody-Icon" src="/-/media/12035-D-mRNA_mRNA-Purification_V2.ashx?la=en&hash=4846501436944B624D17FC5EDF85AB66C83BDE51" /></div> <div class="workflow-icon"><strong> <p1>5. mRNA Purification and QC</p1> </strong> <br /> <p><strong><br /> High-Quality Ribonucleotides: <br /> </strong>RNA product generated from 100% Sanger sequence-verified template with size visualization and certificate of analysis (CoA)</p> </div> </div> </div> </div> <style type="text/css"> .protocol { width: 1170px; display: inline-block; text-align: center; } .protocol img { width: 98%; display: block; margin-left: auto; margin-right: auto; } @media screen and (max-width: 1170px) { .protocol { width: auto; } } </style> <div class="protocol"> <div class="data-container"> <div class="container"> <img alt="" src="/-/media/mRNA-Workflow-Arrow.ashx?w=1170px&la=en&hash=40915FB8A547CDCD8DFB3AE5964A98D19EA782CF" width="1170px" /> </div> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <span id="Featured-Application" class="anchor"></span> <style type="text/css"> body { margin: 0; padding: 0; } .fg-new-container { background: #ffffff; padding: 30px 0; } .fg-new-container h2 { margin: 0; color: #000000; text-align: center; } .fg-new-content p { color: #000000; } .fg-new-content { width: 1170px; margin: 0 auto; } .fg-content-box { display: inline-block; vertical-align: middle; /*width: 50%;*/ } #fg-content-box-left { width: 35%; text-align: left; padding: 30px 0; vertical-align: top; } #fg-content-box-right { width: 49%; text-align: center; } .fg-content-box img { width: 500px; } @media only screen and (max-width: 768px) { .headline-center { padding-top: 0; } .fg-new-container { text-align: center; margin-top: 0; } .fg-new-content { width: 90%; margin: 0 auto; } .fg-new-content { display: inline-block; } #fg-content-box-right, #fg-content-box-left { width: 100%; text-align: center; } #fg-content-box-right { margin-top: 20px; } } @media only screen and (max-width: 1170px) { .fg-new-content { width: 90%; margin: 0 auto; } .fg-content-box img { width: 350px; } } </style> <div class="fg-new-container"> <div class="fg-new-content"> <h2 style="text-align: center;">Featured Application: <span style="text-transform: none;">circ</span>RNA Therapeutic Development</h2> <p> </p> <div class="fg-content-box" id="fg-content-box-left"> <div> </div> <p style="text-align:left;">The circularization of coding RNAs into circRNAs has generated considerable interest as an approach to extend the duration of protein translation, thanks to their covalently closed structures and increased stability inside the cells. In the context of RNA-based therapeutics development, this has the potential to unveil better targets or gene delivery vectors with high pharmaceutical stability and biostability relative to current standard-of-care linear mRNA. Azenta has developed a proprietary and first-in-market workflow to produce high-quality circular RNA. Based on a group I intron self-splicing system, our process leads to improved circularization efficiency for high-yield circRNA products with favorable characteristics for therapeutic development.</p> </div> <div class="fg-content-box" id="fg-content-box-right"> <img alt="FragmentGENE" src="/-/media/mRNA-to-circRNA-Figure4.ashx?h=500&w=300&la=en&hash=A3ECB590BD6842399ED684331A4814423EC5EB14" style="width: 300px; height: 500px;" /> </div> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> .body { text-align: center; display: inline-block; } .banner-background { width: 1170px; background-image: url('/-/media/77005-D_RNA-Therapeutics_WebBanFull-Med_Background.ashx'); background-size: cover; text-align: center; display: flex; padding: 0px; } .banner img { text-align: center; display: block; margin-left: auto; margin-right: auto; } #banner-left { width: 30%; padding: 10px; } #banner-right { width: 69%; padding-top: 20px; padding-right: 0px; padding-left: 100px; padding-bottom: 20px; } #banner-right h1 { text-transform: none; letter-spacing: 1px; line-height: 60px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 55px; margin: 0; margin-bottom: 30px; color: #FFFFFF; } #banner-right h3 { text-transform: none; letter-spacing: 1px; line-height: 35px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 25px; margin: 0; margin-bottom: 20px; color: #FFFFFF; } #banner-right button { padding: 8px 19px; color: #826ec3; letter-spacing: 1px; background: #ffffff; border: 2px solid #ffffff; border-radius: 3px; transition: all .2s ease-in-out; font-size: 15px font-weight: bold; margin-bottom: 10px; } #banner-right button:hover { cursor: pointer; transform: scale(1.1); } #banner-right button:focus { outline: 0; } @media only screen and (max-width: 1170px) { .banner { width: 95%; height: auto; background-image: url('/-/media/77005-D_RNA-Therapeutics_WebBanFull-Med_Background.ashx'); display: inline-block; } #banner-left { text-align: center; width: 100%; } #banner-right { text-align: center; width: 100%; } #banner-right h1 { text-transform: none; letter-spacing: 1px; line-height: 50px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 30px; margin: 0; margin-bottom: 15px; color: #FFFFFF; } #banner-right-1 h3 { text-transform: none; letter-spacing: 1px; line-height: 50px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 10px; margin: 0; margin-bottom: 15px; color: #FFFFFF; } #banner-left img { width: auto; height: auto; } #banner-right img { width: 90%; height: auto; } @media only screen and (max-width: 700px) { #banner-left img { width: 60%; } #banner-right button { font-size: 14px; } } </style> <div class="body"> <div class="banner-background"> <div id="banner-left"> </div> <div id="banner-right"> <div id="banner-right h1"> <h1>Deliver on the promise of<br /> RNA therapeutics</h1> </div> <div id="banner-right h3"> <h3>Explore innovative solutions to support your<br /> RNA therapeutic development research</h3> </div> <div id="banner-right button"> <a rel="noopener noreferrer" href="https://hubs.ly/Q01GDYW60"><button>EXPLORE SOLUTIONS</button></a> </div> </div> </div> </div> </div> <script type="text/javascript" src="/assets/plugins/counter/jquery.counterup.min.js"></script> <script language="javascript" type="text/javascript"> jQuery(document).ready(function ($) { $(".row .category").find("img").addClass("img-responsive"); }); </script> <div class='Component-Frame pull-center Multi-column-TextBullet-Layout Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10'> <div class="container"> <div class="headline-center"> <h2><span style="text-transform: none;">m</span>RNA Synthesis Service Features and Benefits</h2> </div> <div class="headline-left"> </div> <div class="row category"> <div class="col-md-6 col-sm-6"> <div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> <strong>Rapid Project Completion </strong> – Gene synthesis to mRNA IVT in as fast as 3 weeks to meet your project deadlines </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> <strong>High Quality mRNA</strong> - Stringent quality control of IVT template with guaranteed 100% Sanger sequencing accuracy </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> <strong>Ph.D.–Level Customer Support </strong> - Our dedicated project management team provides reliable consultation and support for your project from start to finish </div> </div> </div> <div class="col-md-6 col-sm-6"> <div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> <strong>Effortless Project Management </strong> – Work with a single research partner for everything from construct design to delivery of purified mRNA </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> <strong>Flexible mRNA Delivery </strong> – Suit the needs of your project with a wide range of mRNA types, sequence lengths, and yield of final product </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> <strong>Circular RNA </strong> – Proprietary first-in-market commercial workflow to produce high-quality circular RNA (circRNA) for superior pharmaceutical biostability </div> </div> </div> </div> </div> </div><div class="headline-center Rich-Text-Edit-Area"> <div class="container text-left"> <div class="panel-heading"> </div> <h2 style="text-align: center;"><span style="text-transform: none;">m</span>RNA Synthesis Deliverables</h2> <p> </p> <p>mRNA and circRNA products are provided as dry powder, fully lyophilized and shipped at room temperature. Below is a summary of what to expect from your custom mRNA or circRNA synthesis order.</p> <p> </p> <table class="table table-bordered" style="text-align: center;"> <tbody> <tr> <td style="padding:20px; background-color: #826ec3; color: #FFFFFF;"><strong>mRNA Synthetic Motifs</strong></td> <td style="padding:20px; background-color: #826ec3; color: #FFFFFF;"><strong>Purified Synthetic Yield</strong></td> <td style="padding:20px; background-color: #826ec3; color: #FFFFFF;"><strong>Synthetic Length & Estimated IVT Completion Time</strong></td> <td style="padding:20px; background-color: #826ec3; color: #FFFFFF;"><strong>Quality Control</strong></td> </tr> <tr> <td style="padding:20px; background-color: #826ec3; color: #FFFFFF;">ncRNA: Non-coding/non-capped RNA</td> <td style="padding:70px 20px; background-color: #f0f0f0; color: #000000;" rowspan="4">100 ug<br /> 500 ug<br /> 1 mg<br /> 5 mg<br /> 10 mg<br /> Custom higher yields available</td> <td style="padding:70px 20px; background-color: #f0f0f0; color: #000000;" rowspan="4">< 4000 nt in as few as 1-2 weeks<br /> 4001-6000 nt in as few as 2-3 weeks<br /> > 6001nt, please inquire (custom project)</td> <td style="padding:70px 20px; background-color: #f0f0f0; color: #000000;" rowspan="4">- 100% sequence accuracy from Sanger sequencing of template<br /> - Certificate of analysis (CoA)<br /> - Gel electrophoresis size visualization<br /> - Agilent Bioanalyzer verification (optional)<sup>2</sup></td> </tr> <tr> <td style="padding:20px; background-color: #826ec3; color: #FFFFFF;">Cap1 mRNA: m7GpppNm-capped mRNA with polyA tail</td> </tr> <tr> <td style="padding:20px; background-color: #826ec3; color: #FFFFFF;">Cap0 mRNA: m7Gppp-capped mRNA with polyA tail</td> </tr> <tr> <td style="padding:20px; background-color: #826ec3; color: #FFFFFF;">Pseudouridine modification (optional)<sup>1</sup></td> </tr> <tr> <td style="padding:70px; background-color: #826ec3; color: #FFFFFF;">circRNA</td> <td style="padding:50px 20px; background-color: #f0f0f0; color: #000000;">20 ug<br /> 50 ug<br /> 100 ug<br /> 500 ug<br /> 1 mg<br /> Custom higher yields available</td> <td style="padding:50px 20px; background-color: #f0f0f0; color: #000000;">< 2000 nt in as few as 2-3 weeks<br /> 2001-4000 nt in as few as 3-4 weeks<br /> > 4001nt, please inquire (custom project)</td> <td style="padding:50px 20px; background-color: #f0f0f0; color: #000000;">- 100% sequence accuracy from Sanger sequencing of template<br /> - RNaseR tolerance test<br /> - RT-PCR of circularization junction<br /> - Certificate of analysis (CoA)<br /> - Gel electrophoresis size visualization<br /> - Agilent Bioanalyzer verification (optional)<sup>2</sup></td> </tr> </tbody> </table> <p> </p> <p>Need mRNA fast for your pilot study? Below is a summary of what to expect from your off-the-shelf GFP mRNA order.</p> <p> </p> <table class="table table-bordered" style="text-align: center;"> <tbody> <tr> <td style="padding:20px; background-color: #826ec3; color: #FFFFFF;"><strong>coGFP<sup>3</sup> control RNAs</strong></td> <td style="padding:20px; background-color: #826ec3; color: #FFFFFF;"><strong>Scale</strong></td> <td style="padding:20px; background-color: #826ec3; color: #FFFFFF;"><strong>Estimated Completion Time</strong></td> </tr> <tr> <td style="padding:20px; background-color: #f0f0f0; color: #000000;">Linear with 5’ cap 1 and a ~100A tail</td> <td style="padding:20px; background-color: #f0f0f0; color: #000000;">100 ug</td> <td style="padding:20px; background-color: #f0f0f0; color: #000000;">As fast as 1 week</td> </tr> <tr> <td style="padding:20px; background-color: #f0f0f0; color: #000000;">Circular RNA</td> <td style="padding:20px; background-color: #f0f0f0; color: #000000;">20 ug</td> <td style="padding:20px; background-color: #f0f0f0; color: #000000;">In as few as 2 weeks</td> </tr> </tbody> </table> <p> </p> <p>1. Pseudouridine is a common RNA modification to replace uridine in IVT mRNA. It has been demonstrated to enhance RNA stability and decrease anti-RNA immune response.</p> <p>2. Agilent Bioanalyzer utilizes LabChip technology to automate the measurement of both the concentration and the integrity/purity of mRNA samples.</p> <p>3. Green fluorescent protein (GFP) 2 from Pontellina plumata, also known as ppluGFP2 (<a href="https://pubmed.ncbi.nlm.nih.gov/14963095/">Shagin</a> et al., 2004).</p> <p>If you have custom requests or require any pre-project consultation, please contact our expert <a href="https://share.hsforms.com/1YfPhh73qSVmOtDuJzn1VTg22k3u?__hstc=251210969.74b01e7869fb129981e59ab9ced73ab3.1682339160185.1682416294139.1682427567706.6&__hssc=251210969.147.1682427567706&__hsfp=1574144387">project management team</a>.</p> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> body { margin: 0; } .tech-resources-container { width: 1170px; margin: 0 auto; padding: 20px; } .tech-resources-container h2 { text-align: center; border-bottom: none; } .tech-resource { display: flex; align-items: center; flex-direction: row; margin-bottom: 35px; } .resource { flex-grow: 1; } .resource-right h3, .resource-right p { width: 90%; margin: 0 auto; border-bottom: none; text-align: left; } .resource button { padding: 5px 15px; border: none; border-radius: 4px; background: #826ec3; color: #fff; font-size: 15px; font-weight: lighter; letter-spacing: 1px; margin-top: 15px; -webkit-transition: ease-in-out 0.2s; -moz-transition: ease-in-out 0.2s; -o-transition: ease-in-out 0.2s; transition: ease-in-out 0.2s; } .resource button:hover { background: #6959a1; } @media only screen and (max-width: 800px) { .tech-resource { flex-direction: column; text-align: center; } .resource-right h3, .resource-right p { text-align: center; } } @media only screen and (max-width: 1170px) { .tech-resources-container { width: 80%; margin: 0 auto; } } </style> <div style="width: 100%; background-color: #f0f0f0; padding: 20px; margin: 0;margin-top: -20px;"> <h2>Custom mRNA Synthesis Technical Resources</h2> <div class="tech-resources-container"> <div class="tech-resource"> <div class="resource resource-left"> <img alt="" src="/-/media/12050-D_mRNA-Plasmid-ATN_TechResource-Thumb.ashx?la=en&hash=EFC188F83B25D24E3A7B5A907C1546084E7198C5" /> </div> <div class="resource resource-right"> <h3>Tech Note | High-Fidelity Production of <em>In Vitro</em> Transcription Plasmids with Long Poly(A) Sequences</h3> <p>Poly(A) tail sequences can impact the integrity of your mRNA plasmids for <em>in vitro</em> transcription. While longer poly(A) tails have been shown to increase their stability, tails greater than 100 bases are susceptible to truncations. Learn how the proprietary mRNA plasmid preparation protocol from Azenta can help you generate higher yields and preserve poly(A) tails of greater lengths compared to standard protocols for high-fidelity templates in our tech note. <br /> <a href="https://web.genewiz.com/tech-note/high-fidelity-production-in-vitro-transcription-plasmids" target="_blank"> <button>Download Now</button> </a> </p> </div> </div> <div class="tech-resource"> <div class="resource resource-left"> <img alt="" src="/-/media/12035-D_mRNA-TechNote_TechResource-Thumb.ashx?la=en&hash=7374D900A8377D1ED9AEB60A77370F86EDA2DF8E" /> </div> <div class="resource resource-right"> <h3>Tech Note | mRNA Production via Integrated Gene Synthesis and <em>In Vitro</em> Transcription</h3> <p><em>In vitro</em> transcription (IVT) is a powerful technique to generate messenger RNA (mRNA) for a wide array of research and therapeutic applications. Transfection of mRNA enables very high protein expression, comparable to viral systems, with rapid onset. Read our tech note to learn how IVT, coupled with gene synthesis, can be used to synthesize mRNA of virtually any sequence quickly and efficiently. <br /> <a href="https://web.genewiz.com/tech-note/mrna-production" target="_blank"> <button>Download Now</button> </a> </p> </div> </div> <div class="tech-resource"> <div class="resource resource-left"> <img alt="" src="/-/media/72007-D_mRNA-Poster_TechResource-Thumb.ashx?la=en&hash=A09DB22698F1D99526680DEF4A33D84C06772FBE" /> </div> <div class="resource resource-right"> <h3>Poster | Optimizing mRNA Vaccines and Virus-Mediated CAR T-Cell Therapy for Immuno-Oncology</h3> <p>Lentiviral vectors have proven effective at delivery of genetic material or gene editing technology for <em>ex vivo</em> processing, but the benefits and promise of Adeno-associated virus (AAV) and mRNA tools for <em>in vivo</em> immunotherapy have garnered recent interest. In this poster, we present streamlined approaches for lentiviral, AAV, and mRNA production that improves data quality through innovative synthesis and sequencing methodologies as compared to current standards <br /> <a href="https://web.genewiz.com/poster/mrna-vaccines-virus-car-t-cell-therapy-immuno-oncology" target="_blank"> <button>Download Now</button> </a> </p> </div> </div> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> .body { text-align: center; display: inline-block; } .banner3 { width: 1170px; background-image: url('/-/media/12054-D_Gene-DIscovery-Solutions_WebBanFull-Med_BG.ashx'); background-size: cover; text-align: left; display: flex; padding: 10px; } .banner3 img { text-align: center; display: block; margin-left: auto; margin-right: auto; } #banner3-left { width: 50%; padding: 20px; } #banner3-right { width: 49%; padding: 10px; } #banner3-right h2 { text-transform: none; letter-spacing: 1px; line-height: 50px; 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