GENEWIZ | Sanger Sequencing Universal Primers

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These free universal primers are being updated to reflect the needs of our customers. Users in our new CLIMS Online Ordering and Data Management System have access to the Updated Azenta Universal Primer list (see below). If you are unsure which CLIMS system you are using, please contact our Technical Support team at 877, ext. 2. </p> <br> <p style="font-size: 18px; font-weight: bold;">Primer not listed? <a href="https://www.genewiz.com/en/Public/Services/Oligo">Learn more</a> about Azenta Oligos.</p> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> .body { text-align: center; display: inline-block; } .banner { width: 1170px; background-image: url('/-/media/11034-D_Low-Conc-Plasmids_WebBanFull-Med_Background.ashx'); background-size: cover; text-align: center; display: flex; padding: 20px; } .banner img { text-align: center; display: block; margin-left: auto; margin-right: auto; } #banner-left { width: 0%; padding: 10px; } #banner-right { width: 99%; padding: 0px; } #banner-right h2 { text-transform: none; letter-spacing: 1px; line-height: 40px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 40px; margin: 0; margin-bottom: 10px; color: #FFFFFF } #banner-right h3 { text-transform: none; letter-spacing: 1px; line-height: 20px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 20px; margin: 0; margin-bottom: 15px; color: #FFFFFF } #banner-right button { padding: 8px 19px; color: #826ec3; letter-spacing: 1px; background: #FFFFFF; border: 2px solid #FFFFFF; border-radius: 3px; transition: all .2s ease-in-out; font-size: 15px; font-weight: regular; } #banner-right button:hover { cursor: pointer; transform: scale(1.1); } #banner-right button:focus { outline: 0; } @media only screen and (max-width: 1170px) { .banner { width: 95%; height: auto; background: #6959a1; display: inline-block; } #banner-left { text-align: center; width: 100%; } #banner-right { text-align: center; width: 100%; } #banner-right h2 { text-transform: none; letter-spacing: 1px; line-height: 40px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 45px; margin: 0; margin-bottom: 10px; color: #FFFFFF } #banner-right h3 { text-transform: none; letter-spacing: 1px; line-height: 20px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 20px; margin: 0; margin-bottom: 15px; color: #FFFFFF } #banner-left img { width: auto; height: auto; } #banner-right img { width: 90%; height: auto; } @media only screen and (max-width: 700px) { #banner-left img { width: 60%; } #banner-right button { font-size: 14px; } } </style> <div class="body"> <div class="banner"> <div id="banner-left"> </div> <div id="banner-right"> <div id="banner-right h3"> <h3>Sanger sequencing for low yield plasmid samples</h3> <div id="banner-right h2"> <h2>Low Concentration? No Problem.</h2> <p> </p> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://hubs.ly/Q02kXXzr0" target="_blank"><button>LEARN MORE</button></a> </div> </div> </div> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <style> body { font-family: 'Open Sans', sans-serif; } h1 { color: #0067B1; font-size: 14px; letter-spacing: 1px; text-align: center; } p { text-align: center; font-size: 13px; } .tables-container { width: 100%; margin-bottom: 60px; } .tablesMain { width: 1170px; margin: 0 auto; } .tableBox { display: inline-block; width: 90%; vertical-align: top; } .inlineTable { margin: 0 auto; width: 90%; } table { border: 1px solid #000; border-collapse: collapse; } th { text-align: center; color: #826ec3; background-color: #fff; } tr { border: 1px solid #000; } td { height: 25px; border: 1px solid #000; line-height: 25px word-wrap:break-word; } .table-header { background-color: #0067B1; } .table-header th { padding: 9px; color: #fff; letter-spacing: 1px; font-weight: 200; } .table-name { width: 22%; border-right: none; } .table-length { width: 15%; } .col-extra p { text-align: left; } .red { color: #ec4b35; font-weight: 200; } .green { color: #62b864; font-weight: 200; } .blue { color: #42b2dd; font-weight: 200; } @media only screen and (max-width: 1130px) { .tableBox { display: block; width: 100%; margin: 0 auto; padding-bottom: 20px; } } @media only screen and (max-width: 1300px) { p { font-size: 12px; } } </style> <div class="tables-container" style="overflow-x: auto;"> <div class="tablesMain"> <div class="tableBox"> <table class="inlineTable"> <tbody> <tr class="table-header"> <th colspan="4" style="background-color: #826ec3;">Updated Azenta Universal Primers</th> </tr> <tr> <th scope="col">Name</th> <th scope="col">Length(nt)</th> <th scope="col">Sequences(5'-3')</th> </tr> <tr> <td class="table-name"> <p>T7</p> </td> <td class="table-length"> <p>20</p> </td> <td> <p>TAATACGACTCACTATAGGG</p> </td> </tr> <tr> <td class="table-name"> <p>T7 Term</p> </td> <td class="table-length"> <p>19</p> </td> <td> <p>GCTAGTTATTGCTCAGCGG</p> </td> </tr> <tr> <td class="table-name"> <p>T3</p> </td> <td class="table-length"> <p>20</p> </td> <td> <p>ATTAACCCTCACTAAAGGGA</p> </td> </tr> <tr> <td class="table-name"> <p>SP6</p> </td> <td class="table-length"> <p>19</p> </td> <td> <p>GATTTAGGTGACACTATAG</p> </td> </tr> <tr> <td class="table-name"> <p>U6</p> </td> <td class="table-length"> <p>20</p> </td> <td> <p>GACTATCATATGCTTACCGT</p> </td> </tr> <tr> <td class="table-name"> <p>BGHR</p> </td> <td class="table-length"> <p>18</p> </td> <td> <p>TAGAAGGCACAGTCGAGG</p> </td> </tr> <tr> <td class="table-name"> <p>M13F</p> </td> <td class="table-length"> <p>16</p> </td> <td> <p>GTAAAACGACGGCCAG</p> </td> </tr> <tr> <td class="table-name"> <p>M13R</p> </td> <td class="table-length"> <p>17</p> </td> <td> <p>CAGGAAACAGCTATGAC</p> </td> </tr> <tr> <td class="table-name"> <p>M13-40FOR</p> </td> <td class="table-length"> <p>17</p> </td> <td> <p>GTTTTCCCAGTCACGAC</p> </td> </tr> <tr> <td class="table-name"> <p>M13-48REV</p> </td> <td class="table-length"> <p>20</p> </td> <td> <p>CGGATAACAATTTCACACAG</p> </td> </tr> <tr> <td class="table-name"> <p>CMV-Forward</p> </td> <td class="table-length"> <p>21</p> </td> <td> <p>CGCAAATGGGCGGTAGGCGTG</p> </td> </tr> <tr> <td class="table-name"> <p>5GEX</p> </td> <td class="table-length"> <p>23</p> </td> <td> <p>GGGCTGGCAAGCCACGTTTGGTG</p> </td> </tr> <tr> <td class="table-name"> <p>3GEX</p> </td> <td class="table-length"> <p>23</p> </td> <td> <p>CCGGGAGCTGCATGTGTCAGAGG</p> </td> </tr> <tr> <td class="table-name"> <p>pFastBacF</p> </td> <td class="table-length"> <p>20</p> </td> <td> <p>GGATTATTCATACCGTCCCA</p> </td> </tr> <tr> <td class="table-name"> <p>pFastBacR</p> </td> <td class="table-length"> <p>20</p> </td> <td> <p>CAAATGTGGTATGGCTGATT</p> </td> </tr> <tr> <td class="table-name"> <p>pBAD Forward</p> </td> <td class="table-length"> <p>20</p> </td> <td> <p>ATGCCATAGCATTTTTATCC</p> </td> </tr> <tr> <td class="table-name"> <p>pBAD Reverse</p> </td> <td class="table-length"> <p>18</p> </td> <td> <p>GATTTAATCTGTATCAGG</p> </td> </tr> <tr> <td class="table-name"> <p>EGFP-C-For</p> </td> <td class="table-length"> <p>22</p> </td> <td> <p>CATGGTCCTGCTGGAGTTCGTG</p> </td> </tr> <tr> <td class="table-name"> <p>EGFP-C-REV</p> </td> <td class="table-length"> <p>17</p> </td> <td> <p>GTTCAGGGGGAGGTGTG</p> </td> </tr> <tr> <td class="table-name"> <p>EGFP-N</p> </td> <td class="table-length"> <p>21</p> </td> <td> <p>CGTCGCCGTCCAGCTCGACCA</p> </td> </tr> <tr> <td class="table-name"> <p>SV40pA-R</p> </td> <td class="table-length"> <p>20</p> </td> <td> <p>GAAATTTGTGATGCTATTGC</p> </td> </tr> <tr> <td class="table-name"> <p>ATTB1</p> </td> <td class="table-length"> <p>20</p> </td> <td> <p>GTTTGTACAAAAAAGCAGGC</p> </td> </tr> <tr> <td class="table-name"> <p>ATTB2</p> </td> <td class="table-length"> <p>24</p> </td> <td> <p>CCACTTTGTACAAGAAAGCTGGGT</p> </td> </tr> <tr> <td class="table-name"> <p>ATTL1</p> </td> <td class="table-length"> <p>24</p> </td> <td> <p>CGCGTTAACGCTAGCATGGATCTC</p> </td> </tr> <tr> <td class="table-name"> <p>ATTL2</p> </td> <td class="table-length"> <p>20</p> </td> <td> <p>CATCAGAGATTTTGAGACAC</p> </td> </tr> <tr> <td class="table-name"> <p>ITS1</p> </td> <td class="table-length"> <p>19</p> </td> <td> <p>TCCGTAGGTGAACCTGCGG</p> </td> </tr> <tr> <td class="table-name"> <p>ITS4</p> </td> <td class="table-length"> <p>20</p> </td> <td> <p>TCCTCCGCTTATTGATATGC</p> </td> </tr> <tr> <td class="table-name"> <p>PJET1-2F</p> </td> <td class="table-length"> <p>23</p> </td> <td> <p>CGACTCACTATAGGGAGAGCGGC</p> </td> </tr> <tr> <td class="table-name"> <p>PJET1-2R</p> </td> <td class="table-length"> <p>24</p> </td> <td> <p>AAGAACATCGATTTTCCATGGCAG</p> </td> </tr> </tbody> </table> </div> </div> </div> </div> <link rel="stylesheet" href="/assets/css/footers/footer-v1.css?v=1"> <div class="footer-v1"> <div class="footer"> <div 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