GENEWIZ from Azenta

<!DOCTYPE html>    <html lang="en">  <head> <title>GENEWIZ from Azenta | AAV-ITR</title>  <meta charset="utf-8"> <meta http-equiv="X-UA-Compatible" content="IE=edge,chrome=1"> <meta name="viewport" content="width=device-width, initial-scale=1.0"> <meta name="description" content="GENEWIZ AAV-ITR Sanger Sequencing sequences difficult inverted terminal repeat (ITR) regions of adeno-associated virus (AAV), to expedite screening and validation of leads."> <meta name="keywords" content="genewiz, azenta life sciences, aav-itr sanger sequencing, adeno-associated virus, inverted terminal repeat regions, itr region, sequence whole AAV vectors, viral vector testing, drug discovery pipeline"> <meta name="author" content="">  <link rel="shortcut icon" href="favicon.png">   <link rel="stylesheet" href="/assets/plugins/bootstrap/css/bootstrap.min.css"> <link rel="stylesheet" href="/assets/css/style.css?v=1"> <link rel="stylesheet" href="/assets/css/custom.css?v=1"> <link rel="canonical" href="https://www.genewiz.com/Public/Services/Sanger-Sequencing/AAV-ITR/" /> <link rel="alternate" hreflang="x-default" href="https://www.genewiz.com/Public/Services/Sanger-Sequencing/AAV-ITR" /> <link rel="alternate" hreflang="en-us" href="https://www.genewiz.com/Public/Services/Sanger-Sequencing/AAV-ITR" /> <link rel="alternate" hreflang="en-us" href="https://www.genewiz.com/en/Public/Services/Sanger-Sequencing/AAV-ITR" /> <link rel="alternate" hreflang="en-GB" href="https://www.genewiz.com/en-GB/Public/Services/Sanger-Sequencing/AAV-ITR" /> <script type="text/javascript" src="/sitecore/shell/controls/lib/prototype/prototype.js"></script> <script type="text/javascript" src="/assets/plugins/jquery/jquery.min.js"></script> <script type="text/javascript"> var jQuery = $; //if (window.location.search.indexOf("sc_mode=edit") > 0) { jQuery.noConflict(); //} </script> <script type="text/javascript" src="/assets/plugins/bootstrap/js/bootstrap.min.js"></script> <script type="text/javascript" src="/assets/plugins/back-to-top.js"></script> <script type="text/javascript" src="/assets/js/app.js"></script>   <script> (function (w, d, s, l, i) { w[l] = w[l] || []; w[l].push( { 'gtm.start': new Date().getTime(), event: 'gtm.js' } ); var f = d.getElementsByTagName(s)[0], j = d.createElement(s), dl = l != 'dataLayer' ? 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href='/en/Public/Services/Next-Generation-Sequencing/RNA-Seq' style='color:#585F69;font-size:14px'>RNA-Seq</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Proteomics' style='color:#585F69;font-size:14px'>Proteomics</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/High-Throughput-Gene-Expression-Screening' style='color:#585F69;font-size:14px'>High-Throughput Gene Expression Screening <sup style="color:#826ec3; font-weight: bold;">NEW</sup></a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Single-Cell-Sequencing' style='color:#585F69;font-size:14px'>Single-Cell Sequencing</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Exome-Sequencing' style='color:#585F69;font-size:14px'>Exome Sequencing</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Amplicon-Sequencing-Services' style='color:#585F69;font-size:14px'>Amplicon Sequencing Services</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Standalone-NGS-Solutions' style='color:#585F69;font-size:14px'>Sequencing Only<br></a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Whole-Genome-Sequencing' style='color:#585F69;font-size:14px'>Whole Genome Sequencing</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/High-Throughput-Genotyping' style='color:#585F69;font-size:14px'>CRISPR Validation</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Targeted-Resequencing-Panels' style='color:#585F69;font-size:14px'>Targeted Sequencing</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Metagenomics-Solutions' style='color:#585F69;font-size:14px'>Metagenomics </a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Epigenomics' style='color:#585F69;font-size:14px'>Epigenomics</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Antibody-Discovery' style='color:#585F69;font-size:14px'>Antibody Discovery <sup style="color:#826ec3; font-weight: bold;">NEW</sup></a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Immunogenomics' style='color:#585F69;font-size:14px'>Immunogenomics</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Digital-Spatial-Profiling' style='color:#585F69;font-size:14px'>Digital Spatial Profiling</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/AAV-Genome-Sequencing' style='color:#585F69;font-size:14px'>AAV Genome Sequencing</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Whole-Plasmid-Sequencing-Plasmid-EZ' style='color:#585F69;font-size:14px'>Whole Plasmid Sequencing: Plasmid-EZ <sup style="color:#826ec3; font-weight: bold;">NEW</sup></a></li></ul></div><div class='col-md-3 '><ul class='list-unstyled'><li><a class='GColor' href='/en/Public/Services/Sanger-Sequencing' style='color:#826ec3;font-size:17px'>Sanger Sequencing</a></li><li><a href='/en/Public/Services/Sanger-Sequencing/Sanger-EZ' style='color:#585F69;font-size:14px'>Sanger-EZ <sup style="color:#826ec3; font-weight: bold;">NEW</sup></a></li><li><a href='/en/Public/Services/Sanger-Sequencing/AAV-ITR' style='color:#585F69;font-size:14px'>AAV-ITR Sanger Sequencing</a></li><li><a href='/en/Public/Services/Sanger-Sequencing/Purified-Templates' style='color:#585F69;font-size:14px'>Purified Templates</a></li><li><a href='/en/Public/Services/Sanger-Sequencing/Difficult-Template-Sequencing' style='color:#585F69;font-size:14px'>Difficult Template Sequencing</a></li><li><a href='/en/Public/Services/Sanger-Sequencing/Direct-Colony-Sequencing' style='color:#585F69;font-size:14px'>Direct Colony Sequencing</a></li><li><a href='/en/Public/Services/Sanger-Sequencing/PCR-Purification' style='color:#585F69;font-size:14px'>PCR Purification</a></li><li><a href='/en/Public/Services/Sanger-Sequencing/Primer-Walking' style='color:#585F69;font-size:14px'>Primer Walking</a></li><br><li><a class='GColor' href='/en/Public/Services/Molecular-Genetics' style='color:#826ec3;font-size:17px'>PCR + Sanger Services</a></li><li><a href='/en/Public/Services/Molecular-Genetics/Bacterial-and-Fungal-Identification' style='color:#585F69;font-size:14px'>Bacterial and Fungal Identification</a></li><li><a href='/en/Public/Services/Molecular-Genetics/Mutation-Analysis' style='color:#585F69;font-size:14px'>Mutation Analysis</a></li><li><a href='/en/Public/Services/Molecular-Genetics/SNP-Genotyping' style='color:#585F69;font-size:14px'>SNP Genotyping</a></li><li><a href='/en/Public/Services/Molecular-Genetics/PCR-Plus-Sequencing-CRISPR-Analysis-Package' style='color:#585F69;font-size:14px'>PCR Plus Sequencing CRISPR Analysis Package</a></li><li><a href='/en/Public/Services/Molecular-Genetics/cDNA-Verification' style='color:#585F69;font-size:14px'>Confirmatory Sequencing & Cell Bank Characterization</a></li><li><a href='/en/Public/Services/Molecular-Genetics/Yeast-Colony-Sequencing' style='color:#585F69;font-size:14px'>Yeast Colony Sequencing</a></li><li><a href='/en/Public/Services/Molecular-Genetics/Fragment-Analysis' style='color:#585F69;font-size:14px'>Fragment Analysis</a></li><li><a href='/en/Public/Services/Molecular-Genetics/PCR-Solutions' style='color:#585F69;font-size:14px'>Quantitative PCR & Digital PCR <sup style="color:#826ec3;"><strong>NEW </strong></sup></a></li><li><a href='/en/Public/Services/Molecular-Genetics/Nucleic-Acid-Extraction' style='color:#585F69;font-size:14px'>Nucleic Acid Extraction</a></li></ul></div><div class='col-md-3 '><ul class='list-unstyled'><li><a class='GColor' href='/en/Public/Services/Gene-Synthesis' style='color:#826ec3;font-size:17px'>DNA & RNA Synthesis</a></li><li><a href='/en/Public/Services/Gene-Synthesis/Gene-Synthesis' style='color:#585F69;font-size:14px'>Gene Synthesis</a></li><li><a href='/en/Public/Services/Gene-Synthesis/Antibody-DNA-Synthesis' style='color:#585F69;font-size:14px'>Antibody Synthesis</a></li><li><a href='/en/Public/Services/Gene-Synthesis/AAV-Plasmid-Synthesis' style='color:#585F69;font-size:14px'>AAV Plasmid Synthesis</a></li><li><a href='/en/Public/Services/Gene-Synthesis/Gene-Fragments' style='color:#585F69;font-size:14px'>Gene Fragments</a></li><li><a href='/en/Public/Services/Gene-Synthesis/CRISPR-Construct-Synthesis' style='color:#585F69;font-size:14px'>CRISPR Construct Synthesis</a></li><li><a href='/en/Public/Services/Gene-Synthesis/Synthetic-DNA-Libraries' style='color:#585F69;font-size:14px'>Synthetic DNA Libraries</a></li><li><a href='/en/Public/Services/Gene-Synthesis/Single-Stranded-DNA-Synthesis' style='color:#585F69;font-size:14px'>ssDNA Synthesis</a></li><li><a href='/en/Public/Services/Gene-Synthesis/DNA-Cloning' style='color:#585F69;font-size:14px'>DNA Cloning</a></li><li><a href='/en/Public/Services/Gene-Synthesis/Site-Directed-Mutagenesis' style='color:#585F69;font-size:14px'>Site-Directed Mutagenesis<br></a></li><li><a href='/en/Public/Services/Gene-Synthesis/Viral-Package' style='color:#585F69;font-size:14px'>Viral Packaging <sup style="color:#826ec3;"><strong>NEW </strong></sup></a></li><li><a href='/en/Public/Services/Gene-Synthesis/Recombinant-Antibody-Production' style='color:#585F69;font-size:14px'>Recombinant Antibody Production <sup style="color:#826ec3;"><strong>NEW </strong></sup></a></li><li><a href='/en/Public/Services/Gene-Synthesis/Custom-mRNA-Synthesis' style='color:#585F69;font-size:14px'>Custom mRNA Synthesis <sup style="color:#826ec3; font-weight: bold;">NEW</sup></a></li><br><li><a class='GColor' href='/en/Public/Services/Oligo' style='color:#826ec3;font-size:17px'>Oligo Synthesis Services</a></li><li><a href='/en/Public/Services/Oligo/Overnight-DNA-Oligos' style='color:#585F69;font-size:14px'>Overnight DNA Oligos</a></li><li><a href='/en/Public/Services/Oligo/Custom-DNA-and-RNA-Oligos' style='color:#585F69;font-size:14px'>Custom DNA and RNA Oligos</a></li><li><a href='/en/Public/Services/Oligo/Oligo-Retained-for-Sanger-Sequencing' style='color:#585F69;font-size:14px'>Oligo Retained for Sanger Sequencing</a></li></ul></div><div class='col-md-3 '><ul class='list-unstyled'><li><a class='GColor' href='/en/Public/Services/Plasmid-DNA-Prep' style='color:#826ec3;font-size:17px'>Plasmid DNA Preparation</a></li><li><a href='/en/Public/Services/Plasmid-DNA-Prep/Standard' style='color:#585F69;font-size:14px'>Standard Plasmid DNA Prep</a></li><li><a href='/en/Public/Services/Plasmid-DNA-Prep/Targeted-Yield' style='color:#585F69;font-size:14px'>Targeted Yield Plasmid DNA Prep</a></li><li><a href='/en/Public/Services/Plasmid-DNA-Prep/AAV-Plasmid-Preparation' style='color:#585F69;font-size:14px'>AAV Plasmid Preparation</a></li><br><li><a class='GColor' href='/en/Public/Services/Clinical-Services' style='color:#826ec3;font-size:17px'>PreClinical and Clinical Services</a></li><li><a href='/en/Public/Services/Clinical-Services/Clinical-Services-Overview' style='color:#585F69;font-size:14px'>Clinical Services</a></li><li><a href='/en/Public/Services/Clinical-Services/Microarray-Solutions' style='color:#585F69;font-size:14px'>Microarray Solutions <sup style="color:#826ec3; font-weight: bold;">NEW</sup></a></li><li><a href='/en/Public/Services/Clinical-Services/CLIA-Variant-Confirmation' style='color:#585F69;font-size:14px'>CLIA Variant Confirmation (PCR + Sanger)</a></li><li><a href='/en/Public/Services/Clinical-Services/CLIA-Sanger-Sequencing' style='color:#585F69;font-size:14px'>CLIA Sanger Sequencing</a></li><li><a href='/en/Public/Services/Clinical-Services/CLIA-Whole-Exome-Sequencing' style='color:#585F69;font-size:14px'>CLIA Whole Exome Sequencing</a></li><li><a href='/en/Public/Services/Clinical-Services/CLIA-Whole-Genome-Sequencing' style='color:#585F69;font-size:14px'>CLIA Whole Genome Sequencing</a></li><li><a href='/en/Public/Services/Clinical-Services/Regulatory' style='color:#585F69;font-size:14px'>GLP-Compliant Overview</a></li><li><a href='/en/Public/Services/Clinical-Services/GLP-compliant-Confirmatory-Sequencing' style='color:#585F69;font-size:14px'>GLP Confirmatory Sequencing</a></li><li><a href='/en/Public/Services/Clinical-Services/GLP-compliant-SNP-Mutation-Analysis' style='color:#585F69;font-size:14px'>GLP SNP Mutation Analysis</a></li><li><a href='/en/Public/Services/Clinical-Services/GLP-compliant-Plasmid-Prep' style='color:#585F69;font-size:14px'>GLP Plasmid Prep</a></li><li><a href='/en/Public/Services/Clinical-Services/Nucleic-Acid-Extraction' style='color:#585F69;font-size:14px'>GLP Nucleic Acid Extraction</a></li><li><a href='/en/Public/Services/Clinical-Services/Biofluid-Processing-Overview' style='color:#585F69;font-size:14px'>Biofluid Processing</a></li><li><a href='/en/Public/Services/Clinical-Services/CLIA-Integration-Site-Analysis' style='color:#585F69;font-size:14px'>CLIA Integration Site Analysis <sup style="color:#826ec3; font-weight: bold;">NEW</sup></a></li></ul></div></div></div></li></ul></li><li class='dropdown'><a href='/en/Public/Research-Areas' data-toggle='dropdown' class='dropdown-toggle' style='color:white;height:52px;top:47px;padding-top:0;background-color:transparent;font-size:17px;font-weight:bold;'>RESEARCH AREAS</a><ul class='dropdown-menu'><li class='dropdown'><a class='GColor' href='/en/Public/Research-Areas/AAV' style='font-size:17px'>AAV Services</a><ul class='dropdown-menu'></ul></li><li class='dropdown'><a class='GColor' href='/en/Public/Research-Areas/Agriculture-Food-Science' style='font-size:17px'>Agriculture and Food Science</a><ul class='dropdown-menu'></ul></li><li class='dropdown'><a class='GColor' href='https://web.azenta.com/cell-and-gene-therapy' style='font-size:17px'>Cell and Gene Therapy Solutions 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} .ngs-content-box button { border: none; border-radius: 4px; background: #826ec3; padding: 10px 20px; color: #fff; font-size: 17px; letter-spacing: 1px; } .ngs-content-box button:hover { background: #6965a1; } @media only screen and (max-width: 768px) { .headline-center { padding-top: 0; } .ngs-new-container { text-align: center; margin-top: 0; } .ngs-new-content { width: 90%; margin: 0 auto; } .ngs-new-content { display: inline-block; } #ngs-content-box-right, #ngs-content-box-left { width: 100%; text-align: center; } #ngs-content-box-right { margin-top: 20px; } } @media only screen and (max-width: 1170px) { .ngs-new-content { width: 90%; margin: 0 auto; } .ngs-content-box img { width: 350px; } } </style> <div class="ngs-new-container"> <div class="ngs-new-content"> <div class="ngs-content-box" id="ngs-content-box-left"> <h1>AAV-ITR Sanger Sequencing</h1> <div> </div> <p>Azenta Life Sciences, formerly GENEWIZ’s new proprietary Sanger sequencing method sequences through difficult inverted terminal repeat (ITR) regions of adeno-associated virus (AAV), to expedite screening and validation of leads for your cell and gene therapy research. <br /> <br /> This newly-developed protocol prevents abrupt reduction in the sequencing signal at the start of the ITR hairpin and reads through the full length of the ITR region. AAV-ITR sequencing can be combined with our <a href="/en/Public/Services/Sanger-Sequencing/Primer-Walking">Primer Walking</a> service to sequence whole AAV vectors.</p> <div> </div> <p> </p> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://clims4.genewiz.com/SangerSequencing?lobId=1&serviceId=121" target="_blank"><button>Place an Order</button></a> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://web.genewiz.com/tech-note/aav-itr-sanger-sequencing" target="_blank"><button>AAV-ITR Tech note</button></a> </div> <div class="ngs-content-box" id="ngs-content-box-right"> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://web.genewiz.com/itr50" target="_blank"><img alt="" src="/-/media/Images/AAV_ITR_Banner_Right_Ad_V3.ashx?la=en&hash=FF5B41BCE90C0F5EEC08736D6707B4D58AF4725C" /></a> </div> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <link href="https://fonts.googleapis.com/css?family=Roboto:300,400,500,700" rel="stylesheet" /> <style type="text/css"> .covid-banner { width: 1170px; height: auto; margin: 0 auto; background-image: url("/-/media/11038-D_PEZ-DirectColonySeq_WebBanFull-Med_Background.ashx"); background-size: cover; background-position: center; text-align: left; padding: 0px 0 0px 0; } .covid-content { text-align: center; } .covid-box h3 { color: #ffffff; text-transform: none; letter-spacing: 1px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; line-height: 115%; font-size: 40px; margin: 0; padding-bottom: 10px; padding-top: 15px; } .covid-box h4 { color: #ffffff; text-transform: none; letter-spacing: 1px; line-height: 30px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 35px; margin: 0; margin-bottom: 5px; } .covid-box p { color: #000000; text-transform: none; letter-spacing: 1px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 15px; margin: 0; margin-bottom: 5px; } .covid-box button { padding: 8px 19px; color: #826ec3; letter-spacing: 1px; background: #ffffff; border: 2px solid #ffffff; border-radius: 0px; transition: all .2s ease-in-out; font-size: 15px } .covid-box button:hover { cursor: pointer; transform: scale(1.1); } .covid-box button:focus { outline: 0; } @media only screen and (max-width: 1170px) { .covid-banner { width: 100%; height: auto; } .covid-content { width: 90%; margin: 0 auto; } } </style> <br /> <div class="covid-banner"> <div class="covid-content"> <div class="covid-box"> <br /> <h3><span style="color: #55c8d2;">NEW</span> Direct Colony to<br /> Whole Plasmid Sequencing</h3> <a href="https://hubs.ly/Q02FKv_f0" target="_blank"> <br /> <button> LEARN MORE</button> <br /> </a> <p> </p> </div> </div> </div> <p> </p> </div> <div class="headline-center Rich-Text-Edit-Area"> <div class="container"> <h2>Azenta AAV-ITR Sequencing Provides a Breakthrough Solution</h2> <p>Adeno-associated virus (AAV) is one of the most actively investigated gene therapy vehicles. Azenta’s robust AAV-ITR Sequencing Protocol sequences through its difficult inverted terminal repeat (ITR) regions to better support your viral vector testing and drug discovery pipeline.</p> </div> <br> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> .protocol { width: 1170px; display: inline-block; text-align: center; } .protocol img { width: 98%; height: auto; display: block; margin-left: auto; margin-right: auto; } @media screen and (max-width: 1170px) { .protocol { width: auto; } } </style> <div class="protocol"> <h3 style="text-align: center;"> <strong>Standard Protocol</strong></h3> <a rel="noopener noreferrer" href="https://web.azenta.com/optimizing-aav-plasmid-preparation-and-itr-sequencing" target="_blank"><img alt="GENEWIZ AAV-ITR Protocol" src="/-/media/Images/Graph1.ashx?la=en&hash=FE68D1FC78E00B975E5B35014A5BA26D5C59B9E4" /></a> <br /> <br /> <br /> <h3 style="text-align: center;"> <strong>AAV-ITR Sequencing – GENEWIZ Proprietary Protocol</strong></h3> <a rel="noopener noreferrer" href="https://web.azenta.com/optimizing-aav-plasmid-preparation-and-itr-sequencing" target="_blank"><img alt="GENEWIZ AAV-ITR Protocol" src="/-/media/Images/Graph2.ashx?la=en&hash=2E7FC275D14802557FD682E6FF967052A167ED28" /></a> </div> </div> <script type="text/javascript" src="/assets/plugins/counter/jquery.counterup.min.js"></script> <script language="javascript" type="text/javascript"> jQuery(document).ready(function ($) { $(".row .category").find("img").addClass("img-responsive"); }); </script> <div class='Component-Frame pull-center Multi-column-TextBullet-Layout Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10'> <div class="container"> <div class="headline-center"> <h2> FEATURES & BENEFITS</h2> </div> <div class="headline-left"> </div> <div class="row category"> <div class="col-md-6 col-sm-6"> <div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><em class="BlueCheck icon-custom icon-bg-light-grey icon-4x fa fa-check" style="font-size: 50px;"></em></div> <div class="content-boxes-in-v3"> <strong>Qualitative assessment </strong>of the integrity of wild-type and partially-truncated ITR regions in AAV plasmids <br /> </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><em class="BlueCheck icon-custom icon-bg-light-grey icon-4x fa fa-check" style="font-size: 50px;"></em></div> <div class="content-boxes-in-v3"> <strong>Ph.D.-level </strong><b>customer support</b> throughout the project </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><em class="BlueCheck icon-custom icon-bg-light-grey icon-4x fa fa-check" style="font-size: 50px;"></em></div> <div class="content-boxes-in-v3"> <strong>Turnaround time </strong>starting at just 5 business days </div> </div> </div> <div class="col-md-6 col-sm-6"> <div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><em class="BlueCheck icon-custom icon-bg-light-grey icon-4x fa fa-check" style="font-size: 50px;"></em></div> <div class="content-boxes-in-v3"> <strong>Increased read lengths </strong>and improved data quality for early detection of point mutations </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><em class="BlueCheck icon-custom icon-bg-light-grey icon-4x fa fa-check" style="font-size: 50px;"></em></div> <div class="content-boxes-in-v3"> <strong>Advanced network </strong>of dropboxes and couriers for convenient sample submission </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><em class="BlueCheck icon-custom icon-bg-light-grey icon-4x fa fa-check" style="font-size: 50px;"></em></div> <div class="content-boxes-in-v3"> <strong>Online ordering </strong>system allows real-time order management </div> </div> </div> </div> </div> </div><div class="headline-center Rich-Text-Edit-Area"> <link href="https://fonts.googleapis.com/css?family=Roboto:300,400,500,700" rel="stylesheet" /> <style type="text/css"> .covid-banner2 { width: 1170px; height: auto; margin: 0 auto; background-image: url("/-/media/77007-D-0724-Workshops_Medium_Background.ashx"); background-size: cover; background-position: center; text-align: left; padding: 0px 0 0px 0; } .covid-content2 { text-align: center; } .covid-box2 h3 { color: #ffffff; text-transform: none; letter-spacing: 1px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; line-height: 115%; font-size: 40px; margin: 0; padding-bottom: 10px; padding-top: 90px; } .covid-box2 h4 { color: #ffffff; text-transform: none; letter-spacing: 1px; line-height: 30px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 35px; margin: 0; margin-bottom: 5px; } .covid-box2 p { color: #ffffff; text-transform: none; letter-spacing: 1px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 25px; margin: 0; margin-bottom: 5px; } .covid-box2 button { padding: 8px 19px; color: #826ec3; letter-spacing: 1px; background: #ffffff; border: 2px solid #ffffff; border-radius: 0px; transition: all .2s ease-in-out; font-size: 15px } .covid-box2 button:hover { cursor: pointer; transform: scale(1.1); } .covid-box2 button:focus { outline: 0; } @media only screen and (max-width: 1170px) { .covid-banner { width: 100%; height: auto; background-image: url("/-/media/77007-D-0724-Workshops_Mobile_Background3.ashx"); background-size: cover; background-position: center; text-align: center; padding: 2px 2 2px 2; } .covid-content { width: 90%; margin: 0px 0 0px 0; } .covid-box h3 { color: #ffffff; text-transform: none; letter-spacing: 1px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 350; line-height: 115%; font-size: 35px; margin: 0; padding-bottom: 10px; padding-top: 90px; } .covid-box p { color: #ffffff; text-transform: none; letter-spacing: 1px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 250; font-size: 15px; margin: 0; margin-bottom: 5px; } } </style> <br /> <div class="covid-banner2"> <div class="covid-content2"> <div class="covid-box2"> <h3>Enhancing Gene Transfer Efficiency:<br /> Viral vs. Non-Viral Methods</h3> <p>Friday, September 20, 2024 | 11:00AM EDT</p> <a href="https://hubs.ly/Q02JnzXg0" target="_blank"> <button><strong> REGISTER NOW</strong></button> </a> <p> </p> </div> </div> </div> <p> </p> </div> <script language="javascript" type="text/javascript"> jQuery(document).ready(function ($) { $(".SingleIcon").find("img").addClass("img-responsive center-block"); }); </script> <div class="process-v1 icon-nav Component-BackGround-Gray margin-top-0 margin-bottom-0 padding-top-10 padding-bottom-0"> <div class="container"> <div class="headline-center"> <h2>Technical Resources</h2> <p> </p> </div> <ul class="list-unstyled row "> <li class='col-sm-6'> <a href='https://www.genewiz.com/Public/Resources/Sample-Submission-Guidelines/Sanger-Sequencing-Sample-Submission-Guidelines/Bacteria-Phage-and-bacs#sanger-sequence' target=''> <div class='SingleIcon headline-center Component-BackGround-Default margin-top-0 margin-bottom-0 padding-top-10 padding-bottom-0'> <div class="row"> <img src="/-/media/Images/Home/icons/Purple-Icons/Prepare_Samples_Web-Icon-Workflow_Purple.ashx?h=91&la=en&w=91&hash=46CD2A5C3BAF8BA51A6B26A29368BBB9171BDA5C" alt="Building" width="91" height="91" /> </div> <div class="row"> <h3>Sample Submission Guidelines</h3> </div> <div class="row">   </div> </div> </a> </li>  <li class='col-sm-6'> <a href='https://www.genewiz.com/en/Public/Resources/FAQs/FAQs-DNA-Sequencing' target=''> <div class='SingleIcon headline-center Component-BackGround-Default margin-top-0 margin-bottom-0 padding-top-10 padding-bottom-0'> <div class="row"> <img src="/-/media/Images/Home/icons/Purple-Icons/chat-bubble_Purple.ashx?h=110&la=en&w=210&hash=20D4E093CF40A1D72E7CE3721F0FDEAC239B298C" alt="" width="210" height="110" /> </div> <div class="row"> <h3>Frequently Asked Questions</h3> </div> <div class="row">   </div> </div> </a> </li>  </ul> <div class="row headline-center">   </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> body { margin: 0; } .tech-resources-container { width: 1170px; margin: 0 auto; padding: 0 10 10 10; } .tech-resources-container h2 { text-align: center; border-bottom: none; } .tech-resource { display: flex; align-items: center; flex-direction: row; margin-bottom: 35px; } .resource { flex-grow: 1; } .resource-right h3, .resource-right p { width: 90%; margin: 0 auto; border-bottom: none; text-align: left; } .resource button { padding: 5px 15px; border: none; border-radius: 4px; background: #826ec3; color: #fff; font-size: 15px; font-weight: lighter; letter-spacing: 1px; margin-top: 15px; -webkit-transition: ease-in-out 0.2s; -moz-transition: ease-in-out 0.2s; -o-transition: ease-in-out 0.2s; transition: ease-in-out 0.2s; } .resource button:hover { background: #6959a1; } @media only screen and (max-width: 800px) { .tech-resource { flex-direction: column; text-align: center; } .resource-right h3, .resource-right p { text-align: center; } } @media only screen and (max-width: 1170px) { .tech-resources-container { width: 80%; margin: 0 auto; } } </style> <div style="width: 100%; background-color: #f1f0f0; padding: 10px; padding-top: -20px; margin: 0;margin-top: -20px;"> <div class="tech-resources-container"> <div class="tech-resource"> <div class="resource resource-left"> <img alt="Tech Note: An Efficient and Reliable Approach to AAV Packaging" src="/-/media/12051-D_AAV-Packaging_TechResource-Thumb.ashx?h=160&w=220&la=en&hash=B13220BDC7AA1F511A6C578121266C42359E7B9E" /> </div> <div class="resource resource-right"> <h3>Tech Note: An Efficient and Reliable Approach to AAV Packaging</h3> <p> </p> <p>While AAV is a powerful vehicle for gene delivery, unstable ITRs threaten the fidelity of rAAV genomes. Loss of ITR integrity diminishes the packaging efficiency of rAAV particles and thus viral titer. In this tech note, discover the effect of partial and full ITR deletions on AAV packaging and how to reliably identify and effectively correct them.<br /> <a href="https://web.genewiz.com/tech-note/aav-packaging" target="_blank"> <button>Download Now</button> </a> </p> </div> </div> <div class="tech-resource"> <div class="resource resource-left"> <img alt="Webinar: Effective and High-Quality Solutions for AAV-Based Therapies" src="/-/media/Images/Services/AAV/Webinar_AAV_Web-Resource_thumb-July16_2020.ashx?h=160&w=220&la=en&hash=8C59C36121A2809BBD055B819955CF3AF880216F" /> </div> <div class="resource resource-right"> <h3>Webinar: Effective and High-Quality Solutions for AAV-Based Therapies</h3> <p> </p> <p>In this webinar, Azenta’s Dr. Eric Zhao will present our unique AAV-ITR Sanger sequencing and AAV plasmid preparation capabilities that maintain ITR integrity for downstream virus production, as well as our next generation sequencing-enabled QC of packaged rAAV vectors and host genomes using long-read and short-read sequencing technologies. <br /> <a href="https://web.genewiz.com/solutions-for-aav-based-therapies-webinar" target="_blank"> <button>Download</button> </a> </p> </div> </div> <div class="tech-resource"> <div class="resource resource-left"> <img alt="AAV ITR Sequencing Case Study" src="/-/media/Images/Resources/Educational-Resources/AAV_ITR_Sequencing_Thumbnail.ashx?h=160&w=220&la=en&hash=8AA81CF4A2635593134B1E6A71BF3D3F9153BDBB" /> </div> <div class="resource resource-right"> <h3>Tech Note: Reading Through the Inverted Terminal Repeats (ITRs) of Adeno-associated Virus (AAV)</h3> <p> </p> <p>GENEWIZ has developed a high-quality, direct Sanger sequencing method that reads through both intact and commonly mutated inverted terminal repeat (ITR) regions of adeno-associated virus (AAV). This method is an effective tool for assessing the integrity of ITRs in AAV plasmids.<br /> <a href="https://web.genewiz.com/tech-note/aav-itr-sanger-sequencing" target="_blank"> <button>Download</button> </a> </p> </div> </div> <div class="tech-resource"> <div class="resource resource-left"> <img height="160" alt="AAV ITR Sequencing Case Study" width="220" src="/-/media/Images/AAV-ITR-Sequencing-Thumbnail-Genewiz-Page.ashx?la=en&hash=A57F1BE9B9559EB20CDC590C61C9E64230942789" /> </div> <div class="resource resource-right"> <h3>Article: Optimizing AAV Plasmid Preparation and ITR Sequencing</h3> <p> </p> <p>The adeno-associated virus (AAV) is a powerful vehicle for gene therapy; however, working with AAV vectors can be challenging. Spontaneous mutations in the inverted terminal repeat (ITR) regions can accumulate during plasmid propagation in bacteria. Find out how our scientists have optimized the AAV plasmid preparation process to deliver 100% accurate constructs. <br /> <a href="https://blog.genewiz.com/optimizing-aav-plasmid-preparation-and-itr-sequencing" target="_blank"> <button>Read Article</button> </a> </p> </div> </div> </div> </div> </div> <script language="javascript" type="text/javascript"> jQuery(document).ready(function ($) { $(".SingleIcon").find("img").addClass("img-responsive center-block"); }); </script> <div class="process-v1 icon-nav Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10"> <div class="container"> <div class="headline-center"> <h2>Related Services</h2> <p> </p> </div> <ul class="list-unstyled row "> <li class='col-sm-3'> <a href='https://www.genewiz.com/en/Public/Services/Plasmid-DNA-Prep/AAV-Plasmid-Preparation' target='_blank'> <div class='SingleIcon headline-center Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10'> <div class="row"> <img src="/-/media/Images/Home/icons/Purple-Icons/GA_AAV_Plasmid_Prep_Webpage_icon_Purple.ashx?h=110&la=en&w=210&hash=475A314BEFF62DEDA1EACB471B03C2066B02B3DB" alt="" width="210" height="110" /> </div> <div class="row"> <br> <strong>AAV Plasmid Preparation</strong> <br> </div> <div class="row"> </div> </div> </a> </li>  <li class='col-sm-3'> <a href='https://web.genewiz.com/aav-plasmid-synthesis' target='_blank'> <div class='SingleIcon headline-center Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10'> <div class="row"> <img src="/-/media/Images/Home/icons/Purple-Icons/GA_AAV_Plasmid_Synthesis_Webpage_Icon_Purple.ashx?h=110&la=en&w=210&hash=893181F3A90931F35F05E4DCA11E1DD3ED957697" alt="" width="210" height="110" /> </div> <div class="row"> <br /> <strong>AAV Plasmid Synthesis</strong> <br /> </div> <div class="row"> </div> </div> </a> </li>  <li class='col-sm-3'> <a href='https://www.genewiz.com/Public/Services/Sanger-Sequencing/Difficult-Template-Sequencing' target=''> <div class='SingleIcon headline-center Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10'> <div class="row"> <img src="/-/media/Images/Home/icons/Purple-Icons/difficult-template-sequencing-110x210_Purple.ashx?h=110&la=en&w=210&hash=64970B95F8298F09414305D67166D7AE00F6D845" alt="" width="210" height="110" /> </div> <div class="row"> <br /> <strong>Difficult Template Sequencing</strong> </div> <div class="row">   </div> </div> </a> </li>  <li class='col-sm-3'> <a href='https://www.genewiz.com/en/Public/Services/Sanger-Sequencing/Premium-Services' target=''> <div class='SingleIcon headline-center Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10'> <div class="row"> <img 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