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(PDF) 3D Hybrid Bioprinting of Macrovascular Structures | Devrim Gozuacik - Academia.edu
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The most common treatments for cardiovascular health diseases are autografts and blood vessel transplantations which has limitations due to lack of donors and the patient's conditions. Although there are several scaffold based studies about vascular tissue engineering, scaffold-based vascular grafts have some side effects including chronic inflammation, thrombosis and rejection after in-vivo implantation. Additionally, there are some problems with cell to cell interaction, the assembly and alignment of ECM components and the host response to scaffolds. Therefore, vascular tissue engineering studies tend towards scaffold-free techniques. In this paper, novel computer aided algorithms and methods are developed for 3D printing of scaffold-free macrovascular structures. An example aorta model is generated using imaging and segmentation software. The developed algorithms are implemented using Rhinoscript. In order to support printed cell aggregates, support structures with 'Cake' and 'Zigzag' patterns are developed and 3D printed.","publication_date":"2013,,","publication_name":"Procedia Engineering","grobid_abstract_attachment_id":"42704315"},"document_type":"paper","pre_hit_view_count_baseline":null,"quality":"high","language":"en","title":"3D Hybrid Bioprinting of Macrovascular Structures","broadcastable":true,"draft":null,"has_indexable_attachment":true,"indexable":true}}["work"]; window.loswp.workCoauthors = [1754489]; window.loswp.locale = "en"; window.loswp.countryCode = "SG"; window.loswp.cwvAbTestBucket = ""; window.loswp.designVariant = "ds_vanilla"; window.loswp.fullPageMobileSutdModalVariant = "control"; window.loswp.useOptimizedScribd4genScript = false; window.loswp.appleClientId = 'edu.academia.applesignon';</script><script defer="" src="https://accounts.google.com/gsi/client"></script><div class="ds-loswp-container"><div class="ds-work-card--grid-container"><div class="ds-work-card--container js-loswp-work-card"><div class="ds-work-card--cover"><div class="ds-work-cover--wrapper"><div class="ds-work-cover--container"><button class="ds-work-cover--clickable js-swp-download-button" data-signup-modal="{"location":"swp-splash-paper-cover","attachmentId":42704315,"attachmentType":"pdf"}"><img alt="First page of “3D Hybrid Bioprinting of Macrovascular Structures”" class="ds-work-cover--cover-thumbnail" src="https://0.academia-photos.com/attachment_thumbnails/42704315/mini_magick20190217-8351-lduvu4.png?1550414885" /><img alt="PDF Icon" class="ds-work-cover--file-icon" src="//a.academia-assets.com/assets/single_work_splash/adobe.icon-574afd46eb6b03a77a153a647fb47e30546f9215c0ee6a25df597a779717f9ef.svg" /><div class="ds-work-cover--hover-container"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">download</span><p>Download Free PDF</p></div><div class="ds-work-cover--ribbon-container">Download Free PDF</div><div class="ds-work-cover--ribbon-triangle"></div></button></div></div></div><div class="ds-work-card--work-information"><h1 class="ds-work-card--work-title">3D Hybrid Bioprinting of Macrovascular Structures</h1><div class="ds-work-card--work-authors ds-work-card--detail"><a class="ds-work-card--author js-wsj-grid-card-author ds2-5-body-md ds2-5-body-link" data-author-id="1754489" href="https://sabanciuniv.academia.edu/devrimgozuacik"><img alt="Profile image of Devrim Gozuacik" class="ds-work-card--author-avatar" src="https://0.academia-photos.com/1754489/2193984/2571019/s65_devrim.gozuacik.jpg" />Devrim Gozuacik</a></div><div class="ds-work-card--detail"><p class="ds-work-card--detail ds2-5-body-sm">2013, Procedia Engineering</p><div class="ds-work-card--work-metadata"><div class="ds-work-card--work-metadata__stat"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">visibility</span><p class="ds2-5-body-sm" id="work-metadata-view-count">…</p></div><div class="ds-work-card--work-metadata__stat"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">description</span><p class="ds2-5-body-sm">10 pages</p></div><div class="ds-work-card--work-metadata__stat"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">link</span><p class="ds2-5-body-sm">1 file</p></div></div><script>(async () => { const workId = 22002515; 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Vascular diseases are mostly treated with autografts and blood vessel transplantations. However, traditional grafting methods have several problems including lack of suitable harvest sites, additional surgical costs for harvesting procedure, pain, infection, lack of donors, and even no substitutes at all. Recently, tissue engineering and regenerative medicine approaches are used to regenerate damaged or diseased tissues. Most of the tissue engineering investigations have been based on the cell seeding into scaffolds by providing a suitable environment for cell attachment, proliferation, and differentiation. Because of the challenges such as difficulties in seeding cells spatially, rejection, and inflammation of biomaterials used, the recent tissue engineering studies focus on scaffold-free techniques. In this paper, the development of novel computer aided algorithms and methods are developed for 3D bioprinting of scaffold-free biomimetic macrovascular structures. Computer model mimicking a real human aorta is generated using imaging techniques and the proposed computational algorithms. An optimized three-dimensional bioprinting path planning are developed with the proposed self-supported model. Mouse embryonic fibroblast (MEF) cell aggregates and support structures (hydrogels) are 3D bioprinted layer-by-layer according to the proposed self-supported method to form an aortic tissue construct.</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"3D Bioprinting of Biomimetic Aortic Vascular Constructs With Self-Supporting Cells","attachmentId":40565058,"attachmentType":"pdf","work_url":"https://www.academia.edu/19338486/3D_Bioprinting_of_Biomimetic_Aortic_Vascular_Constructs_With_Self_Supporting_Cells","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/19338486/3D_Bioprinting_of_Biomimetic_Aortic_Vascular_Constructs_With_Self_Supporting_Cells"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="1" data-entity-id="104474089" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/104474089/3D_Bioprinting_for_Vascularization">3D Bioprinting for Vascularization</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="201065409" href="https://independent.academia.edu/KrupaSudheendra">Krupa Sudheendra</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Bioengineering</p><p class="ds-related-work--abstract ds2-5-body-sm">In the world of clinic treatments, 3D-printed tissue constructs have emerged as a less invasive treatment method for various ailments. Printing processes, scaffold and scaffold free materials, cells used, and imaging for analysis are all factors that must be observed in order to develop successful 3D tissue constructs for clinical applications. However, current research in 3D bioprinting model development lacks diverse methods of successful vascularization as a result of issues with scaling, size, and variations in printing method. This study analyzes the methods of printing, bioinks used, and analysis techniques in 3D bioprinting for vascularization. These methods are discussed and evaluated to determine the most optimal strategies of 3D bioprinting for successful vascularization. Integrating stem and endothelial cells in prints, selecting the type of bioink according to its physical properties, and choosing a printing method according to physical properties of the desired printed ...</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"3D Bioprinting for Vascularization","attachmentId":104194091,"attachmentType":"pdf","work_url":"https://www.academia.edu/104474089/3D_Bioprinting_for_Vascularization","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/104474089/3D_Bioprinting_for_Vascularization"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="2" data-entity-id="12117200" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/12117200/Scaffold_free_vascular_tissue_engineering_using_bioprinting">Scaffold-free vascular tissue engineering using bioprinting</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="30338537" href="https://missouri.academia.edu/GaborForgacs">Gabor Forgacs</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Biomaterials, 2009</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Scaffold-free vascular tissue engineering using bioprinting","attachmentId":46345096,"attachmentType":"pdf","work_url":"https://www.academia.edu/12117200/Scaffold_free_vascular_tissue_engineering_using_bioprinting","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/12117200/Scaffold_free_vascular_tissue_engineering_using_bioprinting"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="3" data-entity-id="125110188" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/125110188/Scaffold_Free_Tubular_Tissues_Created_by_a_Bio_3D_Printer_Undergo_Remodeling_and_Endothelialization_when_Implanted_in_Rat_Aortae">Scaffold-Free Tubular Tissues Created by a Bio-3D Printer Undergo Remodeling and Endothelialization when Implanted in Rat Aortae</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="280332615" href="https://independent.academia.edu/ShigekiMorita">Shigeki Morita</a></div><p class="ds-related-work--metadata ds2-5-body-xs">PloS one, 2015</p><p class="ds-related-work--abstract ds2-5-body-sm">Small caliber vascular prostheses are not clinically available because synthetic vascular prostheses lack endothelial cells which modulate platelet activation, leukocyte adhesion, thrombosis, and the regulation of vasomotor tone by the production of vasoactive substances. We developed a novel method to create scaffold-free tubular tissue from multicellular spheroids (MCS) using a &quot;Bio-3D printer&quot;-based system. This system enables the creation of pre-designed three-dimensional structures using a computer controlled robotics system. With this system, we created a tubular structure and studied its biological features. Using a &quot;Bio-3D printer,&quot; we made scaffold-free tubular tissues (inner diameter of 1.5 mm) from a total of 500 MCSs (2.5× 104 cells per one MCS) composed of human umbilical vein endothelial cells (40%), human aortic smooth muscle cells (10%), and normal human dermal fibroblasts (50%). The tubular tissues were cultured in a perfusion system and implante...</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Scaffold-Free Tubular Tissues Created by a Bio-3D Printer Undergo Remodeling and Endothelialization when Implanted in Rat Aortae","attachmentId":119212145,"attachmentType":"pdf","work_url":"https://www.academia.edu/125110188/Scaffold_Free_Tubular_Tissues_Created_by_a_Bio_3D_Printer_Undergo_Remodeling_and_Endothelialization_when_Implanted_in_Rat_Aortae","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/125110188/Scaffold_Free_Tubular_Tissues_Created_by_a_Bio_3D_Printer_Undergo_Remodeling_and_Endothelialization_when_Implanted_in_Rat_Aortae"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="4" data-entity-id="110395347" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/110395347/Generating_vascular_conduits_from_tissue_engineering_to_three_dimensional_bioprinting">Generating vascular conduits: from tissue engineering to three-dimensional bioprinting</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="40941572" href="https://theconsultationcenter.academia.edu/DavidMulligan">David Mulligan</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Innovative Surgical Science, 2018</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Generating vascular conduits: from tissue engineering to three-dimensional bioprinting","attachmentId":108224996,"attachmentType":"pdf","work_url":"https://www.academia.edu/110395347/Generating_vascular_conduits_from_tissue_engineering_to_three_dimensional_bioprinting","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/110395347/Generating_vascular_conduits_from_tissue_engineering_to_three_dimensional_bioprinting"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="5" data-entity-id="51766218" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/51766218/Mini_review_advances_in_3D_bioprinting_of_vascularized_constructs">Mini-review: advances in 3D bioprinting of vascularized constructs</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="41772878" href="https://ucla.academia.edu/FBilli">Fabrizio Billi</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Biology Direct</p><p class="ds-related-work--abstract ds2-5-body-sm">3D in vitro constructs have gained more and more relevance in tissue engineering and in cancer-modeling. In recent years, with the development of thicker and more physiologically relevant tissue patches, the integration of a vascular network has become pivotal, both for sustaining the construct in vitro and to help the integration with the host tissue once implanted. Since 3D bioprinting is rising to be one of the most versatile methods to create vascularized constructs, we here briefly review the most promising advances in bioprinting techniques.</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Mini-review: advances in 3D bioprinting of vascularized constructs","attachmentId":69343990,"attachmentType":"pdf","work_url":"https://www.academia.edu/51766218/Mini_review_advances_in_3D_bioprinting_of_vascularized_constructs","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/51766218/Mini_review_advances_in_3D_bioprinting_of_vascularized_constructs"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="6" data-entity-id="94264897" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/94264897/Trends_of_3D_bioprinting_in_vascular_surgery">Trends of 3D bioprinting in vascular surgery</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="251881112" href="https://independent.academia.edu/PaulaLealAnaya">Paula Leal Anaya</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Revista Mexicana de Angiolog�a, 2019</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Trends of 3D bioprinting in vascular surgery","attachmentId":96770252,"attachmentType":"pdf","work_url":"https://www.academia.edu/94264897/Trends_of_3D_bioprinting_in_vascular_surgery","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/94264897/Trends_of_3D_bioprinting_in_vascular_surgery"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="7" data-entity-id="104729583" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/104729583/3D_Bioprinting_for_Vascularized_Tissue_Fabrication">3D Bioprinting for Vascularized Tissue Fabrication</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="38841160" href="https://musc.academia.edu/MichaelYost">Michael Yost</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Annals of biomedical engineering, 2016</p><p class="ds-related-work--abstract ds2-5-body-sm">3D bioprinting holds remarkable promise for rapid fabrication of 3D tissue engineering constructs. Given its scalability, reproducibility, and precise multi-dimensional control that traditional fabrication methods do not provide, 3D bioprinting provides a powerful means to address one of the major challenges in tissue engineering: vascularization. Moderate success of current tissue engineering strategies have been attributed to the current inability to fabricate thick tissue engineering constructs that contain endogenous, engineered vasculature or nutrient channels that can integrate with the host tissue. Successful fabrication of a vascularized tissue construct requires synergy between high throughput, high-resolution bioprinting of larger perfusable channels and instructive bioink that promotes angiogenic sprouting and neovascularization. This review aims to cover the recent progress in the field of 3D bioprinting of vascularized tissues. It will cover the methods of bioprinting v...</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"3D Bioprinting for Vascularized Tissue Fabrication","attachmentId":104380918,"attachmentType":"pdf","work_url":"https://www.academia.edu/104729583/3D_Bioprinting_for_Vascularized_Tissue_Fabrication","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/104729583/3D_Bioprinting_for_Vascularized_Tissue_Fabrication"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="8" data-entity-id="61300272" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/61300272/Bioprinting_of_artificial_blood_vessels_current_approaches_towards_a_demanding_goal">Bioprinting of artificial blood vessels: current approaches towards a demanding goal</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="163232618" href="https://independent.academia.edu/KirstenBorchers">Kirsten Borchers</a></div><p class="ds-related-work--metadata ds2-5-body-xs">European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery, 2014</p><p class="ds-related-work--abstract ds2-5-body-sm">Free-form fabrication techniques, often referred to as &#39;3D printing&#39;, are currently tested with regard to the processing of biological and biocompatible materials in general and for fabrication of vessel-like structures in particular. Such computer-controlled methods assemble 3D objects by layer-wise deposition or layer-wise cross-linking of materials. They use, for example, nozzle-based deposition of hydrogels and cells, drop-on-demand inkjet-printing of cell suspensions with subsequent cross-linking, layer-by-layer cross-linking of synthetic or biological polymers by selective irradiation with light and even laser-induced deposition of single cells. The need of vessel-like structures has become increasingly crucial for the supply of encapsulated cells for 3D tissue engineering, or even with regard to future application such as vascular grafts. The anticipated potential of providing tubes with tailored branching geometries made of biocompatible or biological materials pushe...</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Bioprinting of artificial blood vessels: current approaches towards a demanding goal","attachmentId":74371328,"attachmentType":"pdf","work_url":"https://www.academia.edu/61300272/Bioprinting_of_artificial_blood_vessels_current_approaches_towards_a_demanding_goal","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/61300272/Bioprinting_of_artificial_blood_vessels_current_approaches_towards_a_demanding_goal"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="9" data-entity-id="85784203" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/85784203/CAD_Modeling_for_3D_Bio_printing_of_Human_Coronary_Artery">CAD Modeling for 3D Bio-printing of Human Coronary Artery</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="215204341" href="https://independent.academia.edu/TienTungChung">Tien-Tung Chung</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Journal of Image and Graphics, 2017</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"CAD Modeling for 3D Bio-printing of Human Coronary Artery","attachmentId":90379679,"attachmentType":"pdf","work_url":"https://www.academia.edu/85784203/CAD_Modeling_for_3D_Bio_printing_of_Human_Coronary_Artery","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/85784203/CAD_Modeling_for_3D_Bio_printing_of_Human_Coronary_Artery"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div></div></div><div class="ds-sticky-ctas--wrapper js-loswp-sticky-ctas hidden"><div class="ds-sticky-ctas--grid-container"><div class="ds-sticky-ctas--container"><button class="ds2-5-button js-swp-download-button" data-signup-modal="{"location":"continue-reading-button--sticky-ctas","attachmentId":42704315,"attachmentType":"pdf","workUrl":null}">See full PDF</button><button class="ds2-5-button ds2-5-button--secondary js-swp-download-button" data-signup-modal="{"location":"download-pdf-button--sticky-ctas","attachmentId":42704315,"attachmentType":"pdf","workUrl":null}"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">download</span>Download PDF</button></div></div></div><div class="ds-below-fold--grid-container"><div class="ds-work--container js-loswp-embedded-document"><div class="attachment_preview" data-attachment="Attachment_42704315" style="display: none"><div class="js-scribd-document-container"><div class="scribd--document-loading js-scribd-document-loader" style="display: block;"><img alt="Loading..." src="//a.academia-assets.com/images/loaders/paper-load.gif" /><p>Loading Preview</p></div></div><div style="text-align: center;"><div class="scribd--no-preview-alert js-preview-unavailable"><p>Sorry, preview is currently unavailable. 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