微小RNA-128-3p通过调控锌指盒同源结合蛋白1抑制卵巢癌细胞上皮-间充质转化

<!DOCTYPE html> <html> <head> <META NAME="GENERATOR" CONTENT="北京玛格泰克科技发展有限公司"> <meta http-equiv="Content-Type" content="text/html; charset=utf-8"> <meta http-equiv="X-UA-Compatible" content="IE=edge"> <Meta http-equiv="Content-Language" Content="zh-CN">  <meta name="citation_issn" content="0529-1356" /> <meta name="citation_journal_title" xml:lang="zh" content="解剖学报" /> <meta name="citation_journal_title" xml:lang="en" content="Acta Anatomica Sinica" /> <meta name="citation_language" content="zh" /> <meta name="citation_volume" content="53" /> <meta name="citation_issue" content="6" /> <meta name="citation_firstpage" content="762" /> <meta name="citation_id" content="53/6/762" /> <meta name="citation_doi" content="10.16098/j.issn.0529-1356.2022.06.010" /> <meta name="citation_title" xml:lang="zh" content="微小RNA-128-3p通过调控锌指盒同源结合蛋白1抑制卵巢癌细胞上皮-间充质转化" /> <meta name="citation_title" xml:lang="en" content="MicroRNA-128-3p inhibiting epithelial-mesenchymal transition of ovarian cancer cells by regulating zince finger E-bonx binding homeobox 1" /> <meta name="citation_authors" xml:lang="zh" content="徐玉红张慧雅王运根陈君霞" /> <meta name="citation_authors" xml:lang="en" content="XU Yu-hong ZHANG Hui-ya WANG Yun-gen CHEN Jun-xia" /> <meta name="citation_publication_date" content="2022-12-06" /> <meta name="citation_online_date" content="2022-12-06" /> <meta name="citation_keywords" xml:lang="zh" content="微小RNA-128-3P" /> <meta name="citation_keywords" xml:lang="zh" content="锌指E盒同源结合蛋白1" /> <meta name="citation_keywords" xml:lang="zh" content="卵巢癌" /> <meta name="citation_keywords" xml:lang="zh" content="上皮-间充质转化" /> <meta name="citation_keywords" xml:lang="zh" content="实时定量聚合酶链反应" /> <meta name="citation_keywords" xml:lang="zh" content="人" /> <meta name="citation_keywords" xml:lang="en" content="MicroRNA-128-3P" /> <meta name="citation_keywords" xml:lang="en" content="Zincfinger E-box binding homobox 1" /> <meta name="citation_keywords" xml:lang="en" content="Ovarian cancer" /> <meta name="citation_keywords" xml:lang="en" content="Epithelial-mesenchymal transition" /> <meta name="citation_keywords" xml:lang="en" content="Real-time PCR" /> <meta name="citation_keywords" xml:lang="en" content="Human " /> <meta name="Description" xml:lang="zh" content=" 目的探讨微小RNA（miR）-128-3p对卵巢癌细..." /> <meta name="Description" xml:lang="en" content=" Objective To investigate the effect of mircoRNA-128-3p (miR-128-3p)..." /> <meta name="citation_abstract_html_url" xml:lang="zh" content="https://jpxb.bjmu.edu.cn/CN/10.16098/j.issn.0529-1356.2022.06.010" /> <meta name="citation_abstract_html_url" xml:lang="en" content="https://jpxb.bjmu.edu.cn/EN/10.16098/j.issn.0529-1356.2022.06.010" /> <meta name="citation_pdf_url" content="https://jpxb.bjmu.edu.cn/CN/article/downloadArticleFile.do?attachType=PDF&id=2547" /> <meta name="DC.Format" content="text/html" /> <meta name="DC.Language" content="zh" /> <meta name="DC.Title" content="微小RNA-128-3p通过调控锌指盒同源结合蛋白1抑制卵巢癌细胞上皮-间充质转化" /> <meta name="DC.Identifier" content="10.16098/j.issn.0529-1356.2022.06.010" /> <meta name="DC.Contributor" content="徐玉红" /> <meta name="DC.Contributor" content="张慧雅" /> <meta name="DC.Contributor" content="王运根" /> <meta name="DC.Contributor" content="陈君霞" /> <meta name="DC.Date" content="2022-12-06" /> <meta name="DC.Keywords" xml:lang="zh" content="微小RNA-128-3P,锌指E盒同源结合蛋白1,卵巢癌,上皮-间充质转化,实时定量聚合酶链反应,人,"/> <meta name="DC.Keywords" xml:lang="en" content="MicroRNA-128-3P,Zincfinger E-box binding homobox 1,Ovarian cancer,Epithelial-mesenchymal transition,Real-time PCR,Human ,"/> <meta name="robots" content="index" /> <title>微小RNA-128-3p通过调控锌指盒同源结合蛋白1抑制卵巢癌细胞上皮-间充质转化</title>  <link href="https://jpxb.bjmu.edu.cn/js/bootstrap/css/bootstrap.min.css" rel="stylesheet"> <link rel="stylesheet" href="https://jpxb.bjmu.edu.cn/images/0529-1356/css/css.css"> <link href="https://jpxb.bjmu.edu.cn/js/bootstrap/css/font-awesome.min.css" rel="stylesheet">  <script src="https://jpxb.bjmu.edu.cn/js/jquery/jquery.min.js"></script> <script src="https://jpxb.bjmu.edu.cn/js/bootstrap/js/bootstrap.min.js"></script>   <link rel="stylesheet" href="https://jpxb.bjmu.edu.cn/js/colorbox/colorbox.css"> <script src="https://jpxb.bjmu.edu.cn/js/colorbox/jquery.colorbox.js"></script> <script type="text/javascript"> //等比缩放图片 function DrawImage(ImgD,FitWidth,FitHeight){ var image=new Image(); 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</script> </span> </a> </span> </div>  <div class="col-xs-6 col-sm-4 col-md-2 col-lg-2 margin-bottom-10" id="knowledge_map_div" style="display:none;"> <span class="change-section" onmouseover="changeKnowledgeMapImg(2)" onmouseout="changeKnowledgeMapImg(1)"> <a href="../lexeme/showArticleByLexeme.do?articleID=2547" class="black-bg btn-menu"> <i class="fa text-muted"><img src="../../images/knowledge_map1.png" height="40" style="margin:0px;" width="36" id="knowledge_map_img"></i> <h2>可视化</h2> <span class="badge" id="knowledge_map_count">0</span> </a> </span> </div> </div>  </div>  </div> </div> </div> <div class="row" id="pin"> <div class="col-lg-11 col-md-10 col-sm-12 col-sm-12">  <div class="panel-group" id="accordion" role="tablist" aria-multiselectable="true"> <div class="panel panel-default"> <div class="panel-heading" role="tab" id="zhaiyao" onClick="xianshi(this)" style="cursor: pointer;"> <h4 class="panel-title"> <a id="abstract"> 摘要/Abstract </a> </h4> </div> <div id="collapseOne" class="panel-collapse collapse" role="tabpanel" aria-labelledby="headingOne" name="#abstract" style="display:block;"> <div class="panel-body line-height text-justify"> <p><strong>摘要： </strong><div style="text-align:justify;"> 目的探讨微小RNA（miR）-128-3p对卵巢癌细胞上皮-间充质转化（EMT）的影响及其对锌指E盒同源结合蛋白1（ZEB1）的调控机制。  方法通过Real-time PCR技术检测上皮性卵巢癌组织（EOC）及癌旁正常组织（各30例）中miR-128-3p的表达量并观察其是否存在差异；选取2种人卵巢癌细胞系SKOV3和A2780，分别转染miR-128-3p模拟物（miR-128-3p mimic）组和阴性对照模拟物（NC mimic）组，利用Real-time PCR技术检测4组miR-128-3p的表达量并验证干扰效果，用Transwell实验观察4组细胞的迁移及侵袭能力。通过双荧光素酶实验验证miR-128-3p与ZEB1的调控关系，用Western blotting检测过表达miR-128-3p后ZEB1蛋白的表达水平；在SKOV3和A2780细胞系中转染pcDNA-ZEB1使其过表达ZEB1，分为NC mimic组，miR-128-3p mimic组，miR-128-3p mimic+ pcDNA-ZEB1组，用Western blotting检测6组细胞中EMT相关蛋白E-钙黏蛋白(E-cadherin)及波形蛋白(vimentin)的表达水平。  结果 Real-time PCR结果显示，与癌旁组织相比，miR-128-3p在EOC组织中表达下降（<em>P</em><0.01）；miR-128-3p mimic组miR-128-3p相对表达量高于NC mimic组，而迁移细胞数量、侵袭细胞数量低于NC mimic组（<em>P</em><0.01）。双荧光素酶实验结果显示，miR-128-3p对ZEB1存在负向调控作用。SKOV3、A2780细胞中miR-128-3p mimic组的ZEB1蛋白相对表达量均低于NC mimic组，而E-cadhein的蛋白相对表达量均高于NC mimic组（<em>P</em><0.01）。MiR-128-3p mimic+ pcDNA-ZEB1组E-cadhein的蛋白相对表达量低于miR-128-3p mimic组（<em>P</em><0.01）。SKOV3、A2780细胞中miR-128-3p mimic组vimentin的蛋白相对表达量低于NC mimic组，miR-128-3p mimic+ pcDNA-ZEB1组vimentin的蛋白相对表达量高于miR-128-3p mimic组（<em>P</em><0.01）。  结论 MiR-128-3p在上皮性卵巢癌组织中表达下降，其原因可能是通过调控ZEB1影响EMT相关蛋白表达而参与卵巢癌细胞EMT过程。 </div></p> <form name="refForm" action="showCorrelativeArticle.do" method=post target=_blank> <input type="hidden" name="searchSQL" value="" /> <input type=hidden name="keyword" value="" id="keyword"> <input type=hidden name="author" value="" id="author"> <p><strong>关键词: </strong> <a href="#" onClick="searchKeyword('https://jpxb.bjmu.edu.cn','1','微小RNA-128-3P')">微小RNA-128-3P, </a> <a href="#" onClick="searchKeyword('https://jpxb.bjmu.edu.cn','1','锌指E盒同源结合蛋白1')">锌指E盒同源结合蛋白1, </a> <a href="#" onClick="searchKeyword('https://jpxb.bjmu.edu.cn','1','卵巢癌')">卵巢癌, </a> <a href="#" onClick="searchKeyword('https://jpxb.bjmu.edu.cn','1','上皮-间充质转化')">上皮-间充质转化, </a> <a href="#" onClick="searchKeyword('https://jpxb.bjmu.edu.cn','1','实时定量聚合酶链反应')">实时定量聚合酶链反应, </a> <a href="#" onClick="searchKeyword('https://jpxb.bjmu.edu.cn','1','人')">人</a> </p> <p><strong>Abstract: </strong><div style="text-align:justify;"> Objective To investigate the effect of mircoRNA-128-3p (miR-128-3p) on epithelial-mesenchymal transition (EMT) of ovarian cancer cells and its regulatory mechanism on zinc finger E-box binding homobox 1(ZEB1).   Methods Real-time PCR technology was used to detect the expression of miR-128-3p in epithelial ovarian cancer tissue (EOC) and adjacent normal tissue(30 cases each), and to observe whether there was a difference. Two human ovarian cancer cell lines, SKOV3 and A2780, were selected and transfected respectively. MiR-128-3p mimic (miR-128-3p mimic group) and negative control mimic (NC mimic group) were used to detect the expression of miR-128-3p in 4 groups by Real-time PCR to verify the interference effect. Transwell assay was used. The migration and invasion abilities of the four groups of cells were observed. The regulatory relationship between miR-128-3p and ZEB1 was verified by dual luciferase assay, and the expression level of ZEB1 protein after overexpression of miR-128-3p was detected by Western blotting; pcDNA-ZEB1 was transfected into SKOV3 and A2780 cell lines to make it overexpression of ZEB1 was divided into NC mimic group, miR-128-3p mimic group, and miR-128-3p mimic+pcDNA-ZEB1 group. Western blot  ting was used to detect the EMT-related protein E-cadherin in 6 groups of cells and the expression level of vimentin.   Results Real-time PCR result showed that the expression of miR-128-3p in EOC tissues decreased compared with that in adjacent tissues(<em>P</em><0.01); The relative expression of miR-128-3p in the miR-128-3p mimic group was higher than that in the NC mimic group, while the numbers of migrating cells and invasive cells were lower than those in the NC mimic group (P<0.01). The result  of dual luciferase experiments showed that miR-128-3p had a negative regulatory effect on ZEB1. In SKOV3 and A2780 cells, the relative expression of ZEB1 protein in the miR-128-3p mimic group was lower than that in the NC mimic group, while the relative protein expression of Ecadhein was higher than that in the NC mimic group (<em>P</em><0.01). The relative protein expression of E-cadhein in the miR-128-3p mimic+pcDNA-ZEB1 group was lower than that in the miR-128-3p mimic group (<em>P</em><0.01). In SKOV3 and A2780 cells, the relative protein expression of vimentin in the miR-128-3p mimic group was lower than that in the NC mimic group, and the relative p  rotein expression of vimentin in the miR-128-3p mimic+pcDNA-ZEB1 group was higher than that in the miR-128-3p mimic group (<em>P</em><0.01).   Conclusion The expression of miR-128-3p decreases in epithelial ovarian cancer tissues, which may be due to the regulation of ZEB1 to affect the expression of EMT-related proteins and participate in the EMT process of ovarian cancer cells. </div></p> <p><strong>Key words: </strong> <a href="#" onClick="searchEnKeyword('https://jpxb.bjmu.edu.cn','1','MicroRNA-128-3P')">MicroRNA-128-3P, </a> <a href="#" onClick="searchEnKeyword('https://jpxb.bjmu.edu.cn','1','Zincfinger E-box binding homobox 1')">Zincfinger E-box binding homobox 1, </a> <a href="#" onClick="searchEnKeyword('https://jpxb.bjmu.edu.cn','1','Ovarian cancer')">Ovarian cancer, </a> <a href="#" onClick="searchEnKeyword('https://jpxb.bjmu.edu.cn','1','Epithelial-mesenchymal transition')">Epithelial-mesenchymal transition, </a> <a href="#" onClick="searchEnKeyword('https://jpxb.bjmu.edu.cn','1','Real-time PCR')">Real-time PCR, </a> <a href="#" onClick="searchEnKeyword('https://jpxb.bjmu.edu.cn','1','Human ')">Human </a> </p> </form>  <form name="subjectSchemeForm" action="" method=post target=_blank> <input type="hidden" name="searchSQL" /> <input type="hidden" name="language" /> </form>  <p> <strong> 中图分类号:  </strong> </p> <ul class="list-unstyled pacs"> <li> <p> <a href="#" onclick="subjectScheme('https://jpxb.bjmu.edu.cn','R737.31','1');return false;" target="_blank"> R737.31 </a> </p> </li> </ul>  <div class="row hidden-xs hidden-sm"> <div class="col-xs-12 col-sm-2 col-md-2 col-lg-2 margin-bottom-10 text-center"> <div class="text-primary btn-menu"> <h4>引用本文</h4> </div> </div> <div class="col-xs-12 col-sm-10 col-md-10 col-lg-10 margin-bottom-10"> <div class="primary-border"> <p>徐玉红张慧雅王运根陈君霞. 微小RNA-128-3p通过调控锌指盒同源结合蛋白1抑制卵巢癌细胞上皮-间充质转化[J]. 解剖学报, 2022, 53(6): 762-768. </p> <p>XU Yu-hong ZHANG Hui-ya WANG Yun-gen CHEN Jun-xia. MicroRNA-128-3p inhibiting epithelial-mesenchymal transition of ovarian cancer cells by regulating zince finger E-bonx binding homeobox 1[J]. Acta Anatomica Sinica, 2022, 53(6): 762-768.</p> </div> </div> </div> <div class="row hidden-xs hidden-sm"> <div class="col-xs-12 col-sm-2 col-md-2 col-lg-2 margin-bottom-10 text-center"> <div class="btn-menu bs-callout-warning"> <h4>使用本文</h4> </div> </div> <div class="col-xs-12 col-sm-10 col-md-10 col-lg-10 margin-bottom-10"> <div class="primary-border"> <p> <b> <form name=mail action="https://jpxb.bjmu.edu.cn/CN/article/sendMail.jsp" method=post target=_blank> <div class="bshare-custom pull-left"><div class="bsPromo bsPromo2"></div><a title="分享到微信" class="bshare-weixin" href="javascript:void(0);"></a><a title="分享到新浪微博" class="bshare-sinaminiblog" href="javascript:void(0);"></a><a title="分享到QQ空间" class="bshare-qzone"></a><a title="分享到腾讯微博" class="bshare-qqmb"></a><a title="更多平台" class="bshare-more bshare-more-icon more-style-addthis"></a><span class="BSHARE_COUNT bshare-share-count" style="float: none;">0</span></div><script type="text/javascript" charset="utf-8" src="https://static.bshare.cn/b/buttonLite.js#style=-1&uuid=&pophcol=2&lang=zh"></script><script type="text/javascript" charset="utf-8" src="https://static.bshare.cn/b/bshareC0.js"></script>     /   <a href="#" id="collectArticle" class="shouc"></a> <span id="collectCount"></span> /   <a onclick="mail.submit()" href="javascript:void(null)" class="tuij">推荐</a> <input type="hidden" value='我在《解剖学报》上发现了关于“微小RNA-128-3p通过调控锌指盒同源结合蛋白1抑制卵巢癌细胞上皮-间充质转化”的文章，特向您推荐。请打开下面的网址：https://jpxb.bjmu.edu.cn/CN/abstract/abstract2547.shtml' name="neirong"> <input type="hidden" name="thishref" value="https://jpxb.bjmu.edu.cn/CN/abstract/abstract2547.shtml"> <input type="hidden" name="jname" value="解剖学报"> <input type="hidden" name="title" value='微小RNA-128-3p通过调控锌指盒同源结合蛋白1抑制卵巢癌细胞上皮-间充质转化'> <form> </b> </p> <p><b>导出引用管理器</b> <span class="daochu"><a href="https://jpxb.bjmu.edu.cn/CN/article/getTxtFile.do?fileType=EndNote&id=2547" id="ris_export">EndNote</a>|<a href="https://jpxb.bjmu.edu.cn/CN/article/getTxtFile.do?fileType=Ris&id=2547">Reference Manager</a>|<a href="https://jpxb.bjmu.edu.cn/CN/article/getTxtFile.do?fileType=ProCite&id=2547">ProCite</a>|<a id="bibtex_export" href="https://jpxb.bjmu.edu.cn/CN/article/getTxtFile.do?fileType=BibTeX&id=2547">BibTeX</a>|<a href="https://jpxb.bjmu.edu.cn/CN/article/getTxtFile.do?fileType=RefWorks&id=2547">RefWorks</a></span> </p> <p><strong>链接本文:</strong> <a href="https://jpxb.bjmu.edu.cn/CN/10.16098/j.issn.0529-1356.2022.06.010" class="txt_zhaiyao1">https://jpxb.bjmu.edu.cn/CN/10.16098/j.issn.0529-1356.2022.06.010</a> </p> <p> <strong> </strong>              <a href="https://jpxb.bjmu.edu.cn/CN/Y2022/V53/I6/762" class="txt_zhaiyao1"> https://jpxb.bjmu.edu.cn/CN/Y2022/V53/I6/762</a> </p> </div> </div> </div> </div> </div> </div> <div class="panel panel-default" > <div class="panel-heading" role="tab" id="cankaowenxian" onClick="xianshi(this)"> <a name="reference"></a> <h4 class="panel-title"> <a id="reference" class="collapsed" href="javascript:;"> 参考文献 </a> </h4> </div> <div id="collapseThree" class="panel-collapse collapse" role="tabpanel" aria-labelledby="headingThree" style="display: none;"> <div class="panel-body">  <table width="98%" border="0" align="center" cellpadding="0" cellspacing="8"> <tr> <td class="J_author"> <div style="text-align:justify;"> ［1］Gaona LP, Medina LA, Maga PK. 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Tumor suppressor miR-128-3p inhibits metastasis and epithelial-mesenchymal transition by targeting ZEB1 in esophageal squamous-cell cancer［J］. Acta Biochim Biophys Sin(Shanghai), 2018, 50(2): 171-180. </div> <div style="text-align:justify;"> ［9］Bai J, Zhang X, Shi DD, et al. Exosomal miR-128-3p promotes epithelial-to-mesenchymal transition in colorectal cancer cells by targeting FOXO4 via TGF-β/SMAD and JAK/STAT3 Signaling［J］. Front Cell Dev Biol, 2021,9:568738.  </div> <div style="text-align:justify;"> ［10］ Feng W, Guo RX, Zhang DY, et al. Circ-ABCB10 knockdown inhibits the malignant progression of cervical cancer through microRNA-128-3p/ZEB1 axis［J］. Biol Proced Online, 2021,23(1): 17. </div> <div style="text-align:justify;"> ［11］Zhu L, Wang A, Gao M, Duan X, Li Z. LncRNA MIR4435-2HG triggers ovarian cancer progression by regulating miR-1283p/CKD14 axis［J］. Cancer Cell Int, 2020, 20:145. </div> <div style="text-align:justify;"> ［12］Wang Q, Li XP, Zhou X, et al. A single-nucleotide polymorphism in lnc-LAMC2-1:1 interferes with its interaction with miR-128 to alter the expression of deleted in colorectal cancer and its effect on the survival rate of subjects with ovarian cancer［J］. J Cell Biochem, 2020, 129(1): 227-232. </div> <div style="text-align:justify;"> ［13］Shi Y, Yan FG, Wang FP, et al. MiR-128-3p suppresses tumor proliferation and metastasis via targeting CDC6 in hepatocellular carcinoma cells［J］. Tissue Cell, 2021,72:101534. </div> <div style="text-align:justify;"> ［14］Liu YY, Fu XM, Wang XY, et al. Long non-coding RNA OIP5-AS1 facilitates the progression of ovarian cancer via the miR-128-3p/CCNG1 axis.［J］. Mol Med Rep, 2021, 23(5):388-399. </div> <div style="text-align:justify;"> ［15］Elsayed AM, Amero P, Salama SA, et al. Back to the future: rethinking the great potential of lncRNA(S) for optimizing chemotherapeutic response in ovarian cancer［J］. Cancers(Basel), 2020,12(9):2406. </div> <div style="text-align:justify;"> ［16］Gregory PA, Bert AG, Paterson EL, et al. The miR-200 family and miR-205 regulate epithelial to mesenchymal transition by targeting ZEB1 and SIP1［J］. Nat Cell Biol, 2008,10(5):593-601. </div> <div style="text-align:justify;"> ［17］Frixa T, Sacconi A, Cioce M, et al. MicroRNA-128-3p-mediated depletion of Drosha promotes lung cancer cell migration［J］. 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