miRNAscope™ Assay Red for Detecting smRNA

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paragraph--type--hero-banner paragraph--view-mode--full paragraph-background-color--dark"> <div class="banner_background_image no-lazy-load" style="background: linear-gradient(to top, rgba(0, 0, 0, 0.0) 0%, rgba(0, 0, 0, 0.90) 100%), url('https://resources.bio-techne.com/bio-techne-assets/images/banners/spatial-biology-chromeogenic-header-banner-1500x340.png'); background-position: top center; background-repeat: no-repeat; background-size: cover;"> <div> <div class="paragraph paragraph--type--h1-2-2-text-to-media paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:h1_2_2_text_to_media:field_indv_h1"> <div><h1>miRNAscope™ Assay</h1></div> </div> <div class="field_block:paragraph:h1_2_2_text_to_media:field_lg_sngl_frmtd_text_global"> <div><p>For ASO, miRNA, and siRNA Detection</p></div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:h1_2_2_text_to_media:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/banners/sbd-hero-c1-adora2b-c2-cd73-480x480.png" alt="SBD hero foreground ADORA2B-C2 CD73" fetchpriority="high" > </div> </div> </div> </div> </div> </div> </div> </div> </div> </section> </div> </div> </header> </div> <div id="main" class="container-fluid container-wide"> <div class="row"> <div class="col-md-12"> <div> <div id="block-bio-techne-global-breadcrumbs" class="system_breadcrumb_block"> <nav role="navigation" aria-labelledby="system-breadcrumb"> <h2 id="system-breadcrumb" class="visually-hidden">Breadcrumb</h2> <ol> <li> <a href="/">Home</a> </li> <li> <a href="/reagents">Bio-Techne Reagents</a> </li> <li> <a href="/reagents/rnascope-ish-technology">RNAscope ISH Technology</a> </li> <li> MiRNAscope Assay </li> </ol> </nav> </div> </div> </div> </div> <main> <section class="section"> <div> <div id="block-bio-techne-global-content" class="system_main_block"> <article> <div> <div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><a href="#features">Product Features</a> | <a href="#technology">Technology Options</a> | <a href="#resources">Resources</a> | <a href="#testimonials">What Our Customers Are Saying</a> </p><p> </p><p>The highly specific and sensitive miRNAscope™ Assay has been developed by ACD, a Bio-Techne brand, to detect small RNAs with spatial and morphological context at single-cell resolution. Leveraging our <a href="/reagents/rnascope-ish-technology">RNAscope™</a> core technology, the miRNAscope Assay is designed to enable detection of small RNAs including, <a href="/reagents/rnascope-ish-technology/gene-therapy/aso-rnai">antisense oligonucleotides</a> (ASOs), microRNAs (miRNAs), small interfering RNAs (siRNAs), and other smaller RNAs of 17-50 bases.</p></div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><a name="features"></a></p><h2>Product Line Features</h2><ul><li>While microarrays and PCR both provide useful molecular profiles of diseases, important clinically relevant cell and tissue context information is lost along with the spatial variation of gene expression patterns which is maintained and captured using miRNAscope.</li><li>miRNAscope is used to detect and identify cellular subtypes, visualize gene regulation with morphology, validate miRNA <a href="/reagents/rnascope-ish-technology/biomarker-development">biomarkers</a> in intact tissues, assess small RNA therapeutic delivery mechanisms, or evaluate biodistribution and efficacy of therapies.</li></ul></div> </div> </div> <div> <div class="paragraph paragraph--type--header"> <div> <div><a name="technology"></a><h2>Technology Options</h2></div> </div> </div> </div> <div> <div class="paragraph paragraph--type--_-2-media-to-text mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_media_to_text:field_indv_media"> <div> <a href="https://www.bio-techne.com/reagents/rnascope-ish-technology/leica-automation-assays" title="Discover our Assays for the Leica BOND Rx System" > <img src="https://resources.bio-techne.com/bio-techne-assets/images/products/v2_mirnascope-mouse-embryo-mir-124-3p-staining.png" alt="ACD miRNAscope image - Mouse Embryo" loading="lazy" > </a> </div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_media_to_text:field_header_2_global"> <div><h3>miRNAscope Chromogenic Assays</h3></div> </div> <div class="field_block:paragraph:2_2_media_to_text:field_lg_sngl_frmtd_text_global"> <div><ul><li><a href="/p/in-situ-hybridization-assays/mirnascope-hd-reagent-kit-red_324500"><strong>miRNAscope HD Assay Red</strong></a><strong> </strong>enables the detection of miRNAscope, siRNAscope, and ASOs in a manual chromogenic workflow.</li><li><a href="/p/in-situ-hybridization-assays/mirnascope-ls-reagent-kit-red_324600"><strong>miRNAscope LS Reagent Kit Red</strong></a><strong> </strong>enables the detection of miRNAscope, siRNAscope, and ASOs on the <a href="https://www.leicabiosystems.com/us/ihc-ish/ihc-ish-instruments/bond-rx" target="_blank">Leica Biosystems BOND RX</a> research staining platform in an automated chromogenic workflow.</li></ul><p> </p><p> </p><p> </p><p> </p></div> </div> <div class="field_block:paragraph:2_2_media_to_text:field_call_to_action_global"> <a href="/reagents/rnascope-ish-technology/leica-automation-assays" class="btn btn-primary" type="link"> Discover our Assays for the Leica BOND Rx System </a> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--_-2-media-to-text mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_media_to_text:field_indv_media"> <div> <a href="https://www.bio-techne.com/reagents/rnascope-ish-technology/mirnascope/manual-ls" title="Learn More About RNAscope Plus" > <img src="https://resources.bio-techne.com/bio-techne-assets/images/reagents/v5_RNAscope-plus-infographic.png" alt="RNAscope Plus Infographic" loading="lazy" > </a> </div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_media_to_text:field_header_2_global"> <div><h3>RNAscope Plus</h3></div> </div> <div class="field_block:paragraph:2_2_media_to_text:field_lg_sngl_frmtd_text_global"> <div><p>The RNAscope Plus smRNA-RNA assays use a proprietary method of <em>in situ</em> hybridization (ISH) to simultaneously visualize one smRNA target, microRNAs (miRNAs), PIWI-interacting RNAs (piRNAs), short interfering RNAs (siRNAs), or antisense oligonucleotides (ASOs) along with three RNA targets.</p><ul><li><a href="/p/in-situ-hybridization-assays/rnascope-plus-smrna-rna-hd-reagents-kit_322785"><strong>RNAscope Plus smRNA-RNA Assay</strong></a><strong> </strong>enables miRNA, siRNA, and ASO detection in a manual fluorescent workflow.</li><li><a href="/p/in-situ-hybridization-assays/rnascope-plus-smrna-rna-ls-reagents-kit_322786"><strong>RNAscope Plus smRNA-RNA LS Assay</strong></a><strong> </strong>enables miRNA, siRNA, and ASO detection on the Leica Biosystems BOND RX research staining platform in an automated fluorescent workflow.</li></ul><p> </p><p> </p></div> </div> <div class="field_block:paragraph:2_2_media_to_text:field_call_to_action_global"> <a href="/reagents/rnascope-ish-technology/mirnascope/manual-ls" class="btn btn-primary" type="link"> Learn More About RNAscope Plus </a> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--header"> <div> <div><a name="resources"></a><h2>Featured Resources</h2></div> </div> </div> </div> <div> <div class="paragraph paragraph--type--card-paragraph paragraph--view-mode--default card-para-default"> <div class="row card_row"> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/resources/literature/mirnascope-rna-ish-assay-to-detect-aso-mirna-or-sirna-brochure" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: ; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/literature/mi-rna-scope_brochure.jpg" alt="mi-rna-scope_brochure" loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="/resources/literature/mirnascope-rna-ish-assay-to-detect-aso-mirna-or-sirna-brochure" > <h3 class="card_title_htag"> <div>RNA ISH Assay to Detect ASO, miRNA, or siRNA Brochure</div> </h3> </a> <div class="card_description flex-grow-1"> </div> <div class="card-bottom-link"> <div> <a href="/resources/literature/mirnascope-rna-ish-assay-to-detect-aso-mirna-or-sirna-brochure" > Download Brochure </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="https://resources.bio-techne.com/bio-techne-assets/docs/pdfs/flyers/sensitive-assay-for-aso-and-sirna-detection.pdf" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: ; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/literature/mi-rna-scope_flyer.jpg" alt="Sensitive assay for ASO and siRNA Detection" loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="https://resources.bio-techne.com/bio-techne-assets/docs/pdfs/flyers/sensitive-assay-for-aso-and-sirna-detection.pdf" > <h3 class="card_title_htag"> <div>Sensitive Assay for ASO and siRNA Detection Flyer</div> </h3> </a> <div class="card_description flex-grow-1"> </div> <div class="card-bottom-link"> <div> <a href="https://resources.bio-techne.com/bio-techne-assets/docs/pdfs/flyers/sensitive-assay-for-aso-and-sirna-detection.pdf" > Download Flyer </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/resources/literature/mirnascope-in-situ-hybridization-assay-for-robust-detection-of-microRNAs-and-other-small-rna-targets-app-note" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: ; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/literature/mirnascope_application-note.jpg" alt="Introducing the novel miRNAscope™ in situ hybridization assay for the robust detection of microRNAs and other small RNA targets with spatial resolution" loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="/resources/literature/mirnascope-in-situ-hybridization-assay-for-robust-detection-of-microRNAs-and-other-small-rna-targets-app-note" > <h3 class="card_title_htag"> <div>App Note: Introducing miRNAscope in situ hybridization assay</div> </h3> </a> <div class="card_description flex-grow-1"> </div> <div class="card-bottom-link"> <div> <a href="/resources/literature/mirnascope-in-situ-hybridization-assay-for-robust-detection-of-microRNAs-and-other-small-rna-targets-app-note" > Download Application Note </a> </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--card-paragraph paragraph--view-mode--default card-para-default"> <div class="row card_row"> <div class="col-md-6"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/resources/literature/mirnascope-a-novel-technology-for-detection-of-small-regulatory-rnas" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: ; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/literature/mirnascope-a-novel-in-situ-hybridization-technology-meeting-poster-thumbnail.png" alt="miRNAscope™ : a novel in situ hybridization technology for the detection of small regulatory RNAs with spatial and morphological context" loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="/resources/literature/mirnascope-a-novel-technology-for-detection-of-small-regulatory-rnas" > <h3 class="card_title_htag"> <div>Poster: miRNAscope - a novel in situ hybridization (ISH) technology</div> </h3> </a> <div class="card_description flex-grow-1"> </div> <div class="card-bottom-link"> <div> <a href="/resources/literature/mirnascope-a-novel-technology-for-detection-of-small-regulatory-rnas" > Download Poster </a> </div> </div> </div> </div> </div> <div class="col-md-6"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/resources/literature/expression-of-mature-micrornas-mir-1287-5p-and-mir-658-is-upregulated-in-diabetic-kidney-disease-app-note" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: ; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/literature/fl_micro_rna_kidney.jpg" alt="EXPRESSION OF MATURE microRNAS,miR-1287-5p AND miR-658 IS UPREGULATED IN DIABETIC KIDNEY DISEASE (DKD) TISSUES" loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="/resources/literature/expression-of-mature-micrornas-mir-1287-5p-and-mir-658-is-upregulated-in-diabetic-kidney-disease-app-note" > <h3 class="card_title_htag"> <div>App Note: Expression of mature microRNAs in diabetic kidney disease (DKD) tissue</div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>Expression of mature microRNAs, miR-1287-5p, and miR-658 is upregulated in diabetic kidney disease (DKD) tissues.</p></div> </div> <div class="card-bottom-link"> <div> <a href="/resources/literature/expression-of-mature-micrornas-mir-1287-5p-and-mir-658-is-upregulated-in-diabetic-kidney-disease-app-note" > Download Application Note </a> </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--header"> <div> <div><a name="testimonials"></a><h2>Customer Testimonials</h2></div> </div> </div> </div> <div> <div class="testimonial"> <div > <div class="row"> <div class="col-md-4"> <div class="left-title"> <h2>Customer Success 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With their expertise and through clear communication, we developed several assays to detect both RNA and short oligonucleotides in tissue. Compared to standard complementary probes, the signal we obtained using ACD Bio was evident and specific. After seeing the first images, we immediately knew we wanted to use these techniques more frequently."    </div> </div> </div> </div> </div> <div class="carousel-item">  <div class="paragraph paragraph--type--individual-testimonial individual-testimonial paragraph--view-mode--default"> <div class="testimonial-info-group"> <div class="row"> <div class="col-lg-3 offset-lg-1 col-sm-12"> <div class="text-center text-lg-left"> <div class="individual-testimonial__headshot"> <div class="blazy blazy--field blazy--field-indv-img blazy--field-indv-img--default" data-blazy=""> <div data-b-token="b-fe9783b881e" class="media media--blazy media--rendered media--resourcesbio-techne media--bundle--remote-image"><img src='https://resources.bio-techne.com/bio-techne-assets/images/icons/authors-testimonials.png' alt='Author Testimonial Icon'> </div> </div> </div> </div> </div> <div class="col-10 offset-1 col-sm-10 offset-sm-1 col-md-8 offset-md-2 col-lg-6 offset-lg-0"> <div class="testimonial-info"> <div class="individual-testimonial__info"> <span class="individual-testimonial__name"> Leading BioPharma company, NY, USA </span><br/> <span class="individual-testimonial__position"> </span><br/> </div> </div> </div> <div class="col-12 col-sm-12 col-md-12 col-lg-2 quote-column text-center"> <img class="quote" loading="lazy" src="/themes/custom/bio_techne_global/images/testimonials-quote.svg"/> </div> </div> </div> <div class="row"> <div class="col-10 offset-1 col-md-10 offset-md-1 col-sm-10"> <div class="individual-testimonial__testimonial"> "We had a great experience using miRNAscope™ in our compound screening efforts. The technique is incredibly specific and the results are very easy to understand. Also, working with ACD’s team has been extremely beneficial. They communicated with us during the process to ensure we can get the answers to our questions. They generated impeccable tissue stainings!"  </div> </div> </div> </div> </div> </div> </div> </div> <div class="row"> <div class="col-md-12"> <ol class="carousel-indicators"> <li data-target="#carouselIndicators" data-slide-to="0" class="active"> </li> <li data-target="#carouselIndicators" data-slide-to="1"> </li> </ol> </div> </div> <a class="carousel-control-prev" href="#carouselIndicators" role="button" data-slide="prev"> <span class="carousel-control-prev-icon" aria-hidden="true"></span> <span class="sr-only">Previous</span> </a> <a class="carousel-control-next" href="#carouselIndicators" role="button" data-slide="next"> <span class="carousel-control-next-icon" aria-hidden="true"></span> <span class="sr-only">Next</span> </a> </div> </div> </div> </div> </div> </div> <div> <section class="paragraph paragraph--type--full-width-banner-with-image mb-4 paragraph--view-mode--default paragraph-background-color--"> <div class="banner_background_image" data-bgimage="https://resources.bio-techne.com/bio-techne-assets/images/banners/v8_round-black-blue-gradient-banner-1110x350.png" style="background-repeat: no-repeat;"> <div> <div> <div> <div class="paragraph paragraph--type--_-2-text-to-media mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_header_2_global"> <div><h2>Get Started Today. 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miRNAscope™ Assay Red for Detecting smRNA