Secondary Antibodies

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Areas</a> </div> </div> </div> </div> </div> </li> <li class="nav-item dropdown"> <a class="nav-link dropdown-toggle" href="#" id="navbarDropdown-therapeutic-manufacturing" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false"> Therapeutic Manufacturing <span class="icon-bt-icon-caret-cropped menu-caret js-caret d-inline-block"></span> </a> <div class="dropdown-menu dropdown-menu-therapeutic-manufacturing" aria-labelledby="navbarDropdown-therapeutic-manufacturing"> <div class="row"> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Reagents & Kits<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/reagents/cell-culture-reagents/serum-free-animal-free" class="dropdown-item text-wrap" data-drupal-link-system-path="node/3576">Animal-Free Cell Culture</a> <a href="/reagents/elisa-kits" class="dropdown-item text-wrap" data-drupal-link-system-path="node/176">ELISA Kits</a> <a 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href="/gmp-products/ruo-to-gmp" class="dropdown-item text-wrap" data-drupal-link-system-path="node/3421">Transition to GMP </a> <a href="/gmp-products/loa-request" class="dropdown-item text-wrap" data-drupal-link-system-path="node/13326">DMF/LOA Request</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Instruments<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/brands/proteinsimple" class="dropdown-item text-wrap" data-drupal-link-system-path="node/3281">ProteinSimple Instruments</a> <a href="/instruments/ice" class="dropdown-item text-wrap" data-drupal-link-system-path="node/256">iCE Maurice</a> <a href="/instruments/imagers" class="dropdown-item text-wrap" data-drupal-link-system-path="node/536">Imagers</a> <a href="/instruments/luminex" class="dropdown-item text-wrap" data-drupal-link-system-path="node/241">Luminex</a> <a href="/instruments/micro-flow-imaging" class="dropdown-item text-wrap" data-drupal-link-system-path="node/526">Micro-Flow Imaging</a> <a href="/instruments/simple-plex" class="dropdown-item text-wrap" data-drupal-link-system-path="node/541">Simple Plex Ella</a> <a href="/instruments/simple-western" class="dropdown-item text-wrap" data-drupal-link-system-path="node/521">Simple Western</a> <a href="/instruments/single-cell-dispensers" class="dropdown-item text-wrap" data-drupal-link-system-path="node/6361">Single Cell Dispensers</a> <a href="/instruments/single-cell-western" class="dropdown-item text-wrap" data-drupal-link-system-path="node/531">Single Cell Western</a> <a href="/instruments" class="dropdown-item text-wrap" data-drupal-link-system-path="node/6731">View All Instruments</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Applications<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a 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aria-labelledby="navbarDropdown-diagnostics"> <div class="row"> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Controls & Reagents<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/diagnostics/clinical-quality-controls" class="dropdown-item text-wrap" data-drupal-link-system-path="node/7606">Clinical Quality Controls</a> <a href="/diagnostics/molecular-quality-controls" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5596">Molecular Controls</a> <a href="/diagnostics/controls-reagents/rnascope-ish-probe-high-risk-hpv" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5981">RNAscope™ ISH Probe High Risk HPV</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Kits<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/diagnostics/amplidex-genetic-testing-kits" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5581">Genetics</a> <a href="/diagnostics/quantidex-oncology-testing-kits" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5586">Oncology</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Technology<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/diagnostics/exosome" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5486">Exosome Platform</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> OEM Services<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/diagnostics/ivd-assay-development" class="dropdown-item text-wrap" data-drupal-link-system-path="node/10881">IVD Assay Development</a> <a href="/diagnostics/ivd-contract-manufacturing" class="dropdown-item text-wrap" data-drupal-link-system-path="node/7381">IVD Contract Manufacturing</a> <a href="/diagnostics/molecular-quality-controls/oem-and-commercial-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/6781">Molecular Diagnostic OEM Services</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Companion Diagnostics<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/diagnostics/companion-diagnostics" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5631">Companion Diagnostics Services</a> <a href="/diagnostics/companion-diagnostics/multiomics" class="dropdown-item text-wrap" data-drupal-link-system-path="node/10616">Multiomics Platform</a> </div> </div> </div> </div> </div> </li> <li class="nav-item dropdown"> <a class="nav-link dropdown-toggle" href="#" id="navbarDropdown-services" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false"> Services <span class="icon-bt-icon-caret-cropped menu-caret js-caret d-inline-block"></span> </a> <div class="dropdown-menu dropdown-menu-services" aria-labelledby="navbarDropdown-services"> <div class="row"> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Custom Quotes<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/services/bulk-quotes" class="dropdown-item text-wrap" data-drupal-link-system-path="services/bulk-quotes">Bulk Quote Discounts</a> <a href="/services/custom-formulation" class="dropdown-item text-wrap" data-drupal-link-system-path="services/custom-formulation">Custom Formulation Quotes</a> <a href="/reagents/rnascope-ish-technology/probes/custom" class="dropdown-item text-wrap" data-drupal-link-system-path="node/12571">RNAscope Custom Probe Design Quote</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Custom 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Bio-Techne" data-drupal-link-system-path="node/1136">Custom Gene Engineering Services</a> <a href="/services/custom-luminex-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/271">Custom Luminex Services</a> <a href="/services/custom-protein-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/1441">Custom Protein Services</a> <a href="/services/custom-simple-plex-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/6296">Custom Simple Plex Services</a> <a href="/services/custom-targeted-protein-degradation-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/861">Custom Targeted Protein Degradation Services</a> <a href="/services/professional-assay-services-rnascope" class="dropdown-item text-wrap" data-drupal-link-system-path="node/12306">RNAscope Professional Assay Services</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Instrument Services<span 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</div> </li> <li class="nav-item dropdown"> <a class="nav-link dropdown-toggle" href="#" id="navbarDropdown-resources" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false"> Resources <span class="icon-bt-icon-caret-cropped menu-caret js-caret d-inline-block"></span> </a> <div class="dropdown-menu dropdown-menu-resources" aria-labelledby="navbarDropdown-resources"> <div class="row"> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Product & Instrument Tools<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/resources/cofa-finder-tool" class="dropdown-item text-wrap" data-drupal-link-system-path="resources/cofa-finder-tool">Certificate of Analysis (COA) Finder Tool</a> <a href="/resources/calculators" class="dropdown-item text-wrap" data-drupal-link-system-path="node/7416">Calculators</a> <a href="/resources/flow-cytometry-panel-builder" class="dropdown-item text-wrap" 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class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:h1_2_2_text_to_media:field_indv_h1"> <div><h1>Secondary Antibodies</h1></div> </div> <div class="field_block:paragraph:h1_2_2_text_to_media:field_lg_sngl_frmtd_text_global"> <div><p>Secondary antibodies recognize and bind to primary antibodies. They are used in many different experimental methods to help increase effectiveness of the platform. Our portfolio includes over 900 secondary antibodies for 26 reactive species.</p></div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:h1_2_2_text_to_media:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/secondary-antibodies-foreground-142.png" alt="Secondary Antibodies Hero Foreground" fetchpriority="high" > </div> </div> </div> </div> </div> </div> </div> </div> </div> </section> </div> </div> </header> </div> <div id="main" class="container-fluid container-wide"> <div class="row"> <div class="col-md-12"> <div> <div id="block-bio-techne-global-breadcrumbs" class="system_breadcrumb_block"> <nav role="navigation" aria-labelledby="system-breadcrumb"> <h2 id="system-breadcrumb" class="visually-hidden">Breadcrumb</h2> <ol> <li> <a href="/">Home</a> </li> <li> <a href="/reagents">Bio-Techne Reagents</a> </li> <li> <a href="/reagents/antibodies">Antibodies</a> </li> <li> Secondary Antibodies </li> </ol> </nav> </div> </div> </div> </div> <main> <section class="section"> <div> <div id="block-bio-techne-global-content" class="system_main_block"> <article> <div> <div> <div> <div class="paragraph paragraph--type--header"> <div> <div><h2>What Are Secondary Antibodies?</h2></div> </div> </div> </div> <div> <div class="paragraph paragraph--type--_-3-media-to-text paragraph--view-mode--default mb-4 p-3"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--25-75"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:1_3_media_to_text:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/secondary-ab-binding-card.png" alt="Secondary antibodies bind to the immunoglobulin (Ig) domain of the primary antibody and are species and isotype specific." loading="lazy" > </div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:1_3_media_to_text:field_lg_sngl_frmtd_text_global"> <div><p>Secondary antibodies are useful tools that can help increase the effectiveness of a variety of experimental methods including <a href="/applications/western-blotting">Western blot</a>, <a href="/methods/immunohistochemistry">immunohistochemistry</a> (IHC), <a href="/applications/imaging/flow-cytometry">flow cytometry</a>, and <a href="/reagents/elisa-kits">ELISA</a>. They are generated by immunizing host animals with antibodies from a different species. The antibodies that are generated recognize and bind to the immunoglobulin (Ig) domain of the immunizing primary antibody and assist in purifying, sorting, and detecting target antigens. Secondary antibodies are typically conjugated to a label. The downstream application will determine the conjugation state and choice of label of the secondary antibody. </p></div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--header"> <div> <div><h2>Selecting Secondary Antibodies</h2></div> </div> </div> </div> <div> <div class="paragraph paragraph--type--_-1-text-to-media mb-4 paragraph--view-mode--default p-3"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--75-25"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:3_1_text_to_media:field_lg_sngl_frmtd_text_global"> <div><p>Secondary antibodies are selected based on several different factors. Since the secondary antibody needs to recognize and bind to the <a href="/search?keywords=antibodies&category_attr=Primary%20Antibodies">primary antibody</a>, the secondary antibody needs to be specific for the primary antibody’s host species and isotype (e.g., IgG or IgM). Additionally, the secondary antibody should be conjugated with the most appropriate label for the application it is being used in. For example, <a href="/applications/western-blotting">Western blot</a> and <a href="http://methods/immunohistochemistry">IHC</a> can use secondary antibodies conjugated to either chromogenic or fluorescent molecules, while flow cytometry generally uses fluorescently labeled secondary antibodies.</p></div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:3_1_text_to_media:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/antibodies-cd32_pecam-1-wb_card.jpg" alt="Bio-Techne primary and secondary antibodies were used to detect CD32/PECAM-1 in the bEND.3 mouse endothelioma cell line by WB." loading="lazy" > </div> </div> <div class="field_block:paragraph:3_1_text_to_media:field_image_caption"> <div>Detection of CD31/PECAM-1 in the bEND.3 mouse endothelioma cell line by Western blot.</div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--header"> <div> <div><h2>Search Bio-Techne Secondary Antibodies</h2></div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><h4>Search Secondary Antibodies by Reactivity</h4> <figure class="responsive-figure-table"><table class="table table-bordered"> <tbody> <tr> <td> <p><a href="/search/?keywords=antibodies&category_attr=Secondary%20Antibodies&species=Mouse">anti-Mouse</a></p> </td> <td> <p><a href="/t/igg/secondary-antibodies?species=Goat">anti-Goat</a></p> </td> <td> <p><a href="/t/igg/secondary-antibodies?species=Rabbit">anti-Rabbit</a></p> </td> <td> <p><a href="/t/igg/secondary-antibodies?species=Rat">anti-Rat</a></p> </td> </tr> <tr> <td> <p><a href="/t/igg/secondary-antibodies?species=Sheep">anti-Sheep</a></p> </td> <td> <p><a href="/t/igy/secondary-antibodies?species=Chicken">anti-Chicken</a></p> </td> <td> <p><a href="/t/igg/secondary-antibodies?species=Hamster">anti-Hamster</a></p> </td> <td> <p><a href="/t/igg/secondary-antibodies?species=Human">anti-Human</a></p> </td> </tr> </tbody> </table></figure> <h4>Search Secondary Antibodies by Isotype</h4> <figure class="responsive-figure-table"><table class="table table-bordered"> <tbody> <tr> <td> <p><a href="/t/igg?category_attr=Secondary%20Antibodies">IgG</a></p> </td> <td> <p><a href="/t/igm?category_attr=Secondary%20Antibodies">IgM</a></p> </td> <td> <p><a href="/t/igy?category_attr=Secondary%20Antibodies">IgY</a></p> </td> <td> <p><a href="/t/igg2a?category_attr=Secondary%20Antibodies">IgG2A</a></p> </td> <td> <p><a href="/t/igg2b?category_attr=Secondary%20Antibodies">IgG2B</a></p> </td> <td> <p><a href="/t/ige?category_attr=Secondary%20Antibodies">IgE</a></p> </td> </tr> </tbody> </table></figure> <h4>Search Secondary Antibodies by Conjugation</h4> <figure class="responsive-figure-table"><table class="table table-bordered"> <tbody> <tr> <td> <p><a href="/search/?keywords=antibodies&category_attr=Secondary%20Antibodies&conjugate_form=Biotin">Biotin</a></p> </td> <td> <p><a href="/search/?keywords=antibodies&category_attr=Secondary%20Antibodies&conjugate_form=Allophycocyanin">Allophycocyanin (APC)</a></p> </td> <td> <p><a href="/search/?keywords=antibodies&category_attr=Secondary%20Antibodies&conjugate_form=Phycoerythrin">Phycoerythrin (PE)</a></p> </td> <td> <p><a href="/search/?keywords=antibodies&category_attr=Secondary%20Antibodies&conjugate_form=HRP%20(Horseradish%20Peroxidase)">Horseradish Peroxidase (HRP)</a></p> </td> <td> <p><a href="/search/?keywords=antibodies&category_attr=Secondary%20Antibodies&conjugate_form=Fluorescein">Fluorescein</a></p> </td> </tr> <tr> <td> <p><a href="/search/?keywords=antibodies&category_attr=Secondary%20Antibodies&conjugate_form=NL493">NorthernLights<sup>TM</sup> 493 (NL493)</a></p> </td> <td> <p><a href="/search/?keywords=antibodies&category_attr=Secondary%20Antibodies&conjugate_form=NL557">NorthernLights 557 (NL557)</a></p> </td> <td> <p><a href="/search/?keywords=antibodies&category_attr=Secondary%20Antibodies&conjugate_form=NL637">NorthernLights 637 (NL637)</a></p> </td> <td> <p><a href="/search/?keywords=antibodies&category_attr=Secondary%20Antibodies&conjugate_form=PerCP%20(Peridinin-chlorophyll%20Protein%20Complex)">Peridinin-Chlorophyll Protein Complex (PerCP)</a></p> </td> <td> <p><a href="/search/?keywords=antibodies&category_attr=Secondary%20Antibodies&conjugate_form=Unconjugated">Unconjugated</a></p> </td> </tr> </tbody> </table></figure></div> </div> </div> <div> <div class="thin-banner banner_wrapper"> <div class="banner-dark"> <div> <div>Expedite Your Research. Partner with Us.</div> <a href="/services/antibodies" > <button type="button" class="btn btn-primary"> Request a Custom Antibody Services Quote </button> </a> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--header"> <div> <div><h2>Advantages of Using Secondary Antibodies</h2></div> </div> </div> </div> <div> <div class="paragraph paragraph--type--_-3-media-to-text paragraph--view-mode--default mb-4 p-3"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--25-75"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:1_3_media_to_text:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/graphs/rituximab-biosimilar-graph.jpg" alt="Bio-Techne primary and secondary antibodies were used to detect ACE-2 and CD14 in human PBMCs by flow cytometry." loading="lazy" > </div> </div> <div class="field_block:paragraph:1_3_media_to_text:field_image_caption"> <div>Detection of ACE-2 and CD14 in human PBMCs by flow cytometry.</div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:1_3_media_to_text:field_lg_sngl_frmtd_text_global"> <div><p>Indirect detection of target analytes using the combination of primary and secondary antibodies offers multiple advantages over directly detecting the proteins using only conjugated primary antibodies.</p> <p><strong>•  Amplifies the Signal</strong></p> <p>Multiple secondary antibodies can bind to a single <a href="/search?keywords=antibodies&category_attr=Primary%20Antibodies">primary antibody</a>. Each bound secondary antibody is conjugated to a reporter molecule. This results in an amplification of the signal and an increase in the sensitivity of the assay. For some assays, the signal can be increased further using a <a href="/search?keywords=antibodies&category_attr=Secondary%20Antibodies&conjugate_form=Biotin">biotinylated secondary antibody</a> and the streptavidin-conjugated reporter molecule.</p> <p><strong>• Allows for Multiplexing Experiments</strong></p> <p>Since secondary antibodies are species specific, experiments can be designed to detect multiple analytes using primary antibodies derived from different species. The different analytes are detected using secondary antibodies conjugated to fluorochromes that emit light at different wavelengths.</p> <p><strong>• Preserves the Paratope</strong></p> <p>Conjugating a primary antibody to a reporter molecule has the potential of compromising its antigen-binding site, also called its paratope. This would reduce the primary antibody’s ability to bind to its target antigen. Using a conjugated secondary antibody allows an experimenter to use an unconjugated primary antibody in their assay.</p></div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--card-paragraph paragraph--view-mode--default card-para-default"> <h2>Associated Products</h2> <div class="row card_row"> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/search?keywords=visucyte" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: ; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/antibodies-visucyte-hrp_card.jpg" alt="Bio-Techne offers R&D Systems secondary antibodies conjugated to the VisUCyte HRP Polymer for cleaner, faster IHC results." loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="/search?keywords=visucyte" > <h3 class="card_title_htag"> <div><h5>VisUCyte™ HRP Polymer</h5></div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>Bio-Techne’s VisUCyte HRP Polymer Antibodies eliminate the need to use Avidin-Biotin detection chemistry, thus providing cleaner and faster IHC staining of your tissue.</p></div> </div> <div class="card-bottom-link"> <div> <a href="/search?keywords=visucyte" > View VisUCyte HRP Polymer Antibodies & Kits </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/search?keywords=staining%20kits&category_attr=Staining%20Kits%20and%20Reagents" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: ; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/cell-tissue-staining-kit_card.jpg" alt="Bio-Techne offers R&D Systems Cell & Tissue Staining Kits were used to get optimized staining of GALNT10 in mouse testis tissue." loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="/search?keywords=staining%20kits&category_attr=Staining%20Kits%20and%20Reagents" > <h3 class="card_title_htag"> <div><h5>Cell & Tissue Staining Kits</h5></div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>Bio-Techne’s Cell & Tissue Staining Kits are ready-to-use kits that contain all the reagents needed for IHC staining of tissue sections and cytospin preparations.</p></div> </div> <div class="card-bottom-link"> <div> <a href="/search?keywords=staining%20kits&category_attr=Staining%20Kits%20and%20Reagents" > View Cell & Tissue Staining Kits </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/search?keywords=antibodies&category_attr=Isotype%20Controls" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: ; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/isotype-control-ab-card.png" alt="Bio-Techne offers a wide variety of isotype control options that cover multiple species, isoforms and conjugates to match primary antibodies." loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="/search?keywords=antibodies&category_attr=Isotype%20Controls" > <h3 class="card_title_htag"> <div><h5>Isotype Control Antibodies</h5></div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>Isotype control antibodies are negative controls that help researchers interpret their results. Bio-Techne offers a wide range of isotype control antibodies to match the species, isoform, and conjugation of your primary antibody.</p></div> </div> <div class="card-bottom-link"> <div> <a href="/search?keywords=antibodies&category_attr=Isotype%20Controls" > View Isotype Control Antibodies </a> </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--card-paragraph paragraph--view-mode--default card-para-default"> <h2>Additional Resources</h2> <div class="row card_row"> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/resources/literature/secondary-antibody-handbook" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: ; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/literature/secondary-ab_brochure.png" alt="Novus Biologicals Secondary Antibody Handbook provides valuable information to help you choose a secondary antibody for your experiments." loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="/resources/literature/secondary-antibody-handbook" > <h3 class="card_title_htag"> <div><h5>Secondary Antibodies Handbook</h5></div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>This handbook offers information you need for choosing the best secondary antibody for your experiment.</p></div> </div> <div class="card-bottom-link"> <div> <a href="/resources/literature/secondary-antibody-handbook" > Request the Secondary Antibodies Handbook </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="https://info.bio-techne.com/flow-cytometry-handbook.html" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: ; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/image-flow-cytometry-handbook_480x485_Feb2023Update.png" alt="View of the Flow cytometry handbook and a single cell as a background" loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="https://info.bio-techne.com/flow-cytometry-handbook.html" > <h3 class="card_title_htag"> <div><h5>Flow Cytometry Handbook</h5></div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>This easy-to-follow guide provides an overview of the flow cytometry technique, as well as step-by-step protocols and troubleshooting tips.</p></div> </div> <div class="card-bottom-link"> <div> <a href="https://info.bio-techne.com/flow-cytometry-handbook.html" > Download the Flow Cytometry Handbook </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/resources/literature/immunohistochemistry-ihc-handbook" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: ; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/ihc_brochure_new.png" alt="View of the Immunohistochemistry (IHC) Handbook from BioTechne" loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="/resources/literature/immunohistochemistry-ihc-handbook" > <h3 class="card_title_htag"> <div><h5>Immunohistochemistry (IHC) Handbook</h5></div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>This comprehensive guide provides an introduction to the techniques, protocols, and troubleshooting methods that lead to a successful IHC experiment.</p></div> </div> <div class="card-bottom-link"> <div> <a 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