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Micro-Flow Imaging Instruments | Bio-Techne
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data-drupal-link-system-path="node/5486">Exosome Platform</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> OEM Services<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/diagnostics/ivd-assay-development" class="dropdown-item text-wrap" data-drupal-link-system-path="node/10881">IVD Assay Development</a> <a href="/diagnostics/ivd-contract-manufacturing" class="dropdown-item text-wrap" data-drupal-link-system-path="node/7381">IVD Contract Manufacturing</a> <a href="/diagnostics/molecular-quality-controls/oem-and-commercial-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/6781">Molecular Diagnostic OEM Services</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Companion Diagnostics<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a 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data-drupal-link-system-path="services/bulk-quotes">Bulk Quote Discounts</a> <a href="/services/custom-formulation" class="dropdown-item text-wrap" data-drupal-link-system-path="services/custom-formulation">Custom Formulation Quotes</a> <a href="/reagents/rnascope-ish-technology/probes/custom" class="dropdown-item text-wrap" data-drupal-link-system-path="node/12571">RNAscope Custom Probe Design Quote</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Custom Services<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/services/custom-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/1891">All Custom Services</a> <a href="/services/custom-antibody-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/906">Custom Antibody Services</a> <a href="/services/custom-cell-and-gene-therapy-services" class="dropdown-item text-wrap" 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class="col sub-menu-wrapper"> <div class="sub-menu-header"> Product & Instrument Tools<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/resources/cofa-finder-tool" class="dropdown-item text-wrap" data-drupal-link-system-path="resources/cofa-finder-tool">Certificate of Analysis (COA) Finder Tool</a> <a href="/resources/calculators" class="dropdown-item text-wrap" data-drupal-link-system-path="node/7416">Calculators</a> <a href="/resources/flow-cytometry-panel-builder" class="dropdown-item text-wrap" data-drupal-link-system-path="node/8821">Flow Cytometry Panel Builder</a> <a href="/luminex-assay-customization-tool" class="dropdown-item text-wrap" data-drupal-link-system-path="luminex-assay-customization-tool">Luminex Assay Customization Tool</a> <a href="/resources/product-insert-lookup-tool" class="dropdown-item text-wrap" data-drupal-link-system-path="resources/product-insert-lookup-tool">Product Insert Lookup 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class="field_block:paragraph:h1_2_2_text_to_media:field_lg_sngl_frmtd_text_global"> <div><h2>Image-Based Particle Analysis for Biopharmaceuticals</h2></div> </div> <div class="field_block:paragraph:h1_2_2_text_to_media:field_call_to_action_global"> <a href="/p/instruments/micro-flow-imaging-mfi/request-quote" class="btn btn-primary" type="link"> Request Pricing </a> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:h1_2_2_text_to_media:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/microFlowSolo02.png" alt="Micro Flow" fetchpriority="high" > </div> </div> </div> </div> </div> </div> </div> </div> </div> </section> </div><!-- end hero-banner --> </div> </header> </div> <div id="main" class="container-fluid container-wide"> <div class="row"> <div class="col-md-12"> <div> <div id="block-bio-techne-global-breadcrumbs" class="system_breadcrumb_block"> <nav role="navigation" aria-labelledby="system-breadcrumb"> <h2 id="system-breadcrumb" class="visually-hidden">Breadcrumb</h2> <ol> <li> <a href="/">Home</a> </li> <li> <a href="/instruments">Instruments</a> </li> <li> Micro-Flow Imaging Instruments </li> </ol> </nav> </div> </div> </div> </div> <main> <section class="section"> <div> <div id="block-bio-techne-global-content" class="system_main_block"> <article> <div> <div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><a href="#instrument">MFI Instruments & Consumables</a> | <a href="#how">How Does it Work?</a> | <a href="#comparison">Compare Instruments</a> | <a href="#applications">Applications</a></p> <p> </p> <p>MFI™ gives you more insight into the world of sub-visible particles—way more than just size and count. Because it's image-based, you also get quantifiable morphological parameters for your analysis. Easily differentiate and characterize different particle populations in no time. Plus, MFI has an optic focal length that spans the entire fluidic path, so your sample data is robust and reproducible day in and day out. Add a Bot1 anytime to automate the whole process and kick your throughput up a notch. If you're looking for broader, deeper analysis of your biotherapeutic products, MFI is the particle analysis tool for you.</p> <p>ProteinSimple is now a brand in the Bio-Techne family of brands that includes R&D Systems, Novus Biologicals and Tocris. As a company, Bio-Techne has developed, manufactured and sold biotechnology products, clinical calibrators and controls, and consumables for protein analysis—and now analytical instrumentation and consumables through ProteinSimple. </p> <p><a name="instrument"></a></p></div> </div> </div> <div> <div class="paragraph paragraph--type--card-paragraph paragraph--view-mode--default card-para-default"> <h2>MFI Instruments</h2> <div class="row card_row"> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/p/micro-flow-imaging-mfi/mfi-5000-series_4000-011-001" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: ; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/instruments/proteinsimple-micro-flow-imaging-mfi-495.jpg" alt="Micro Flow Imaging (MFI) Instrument" loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="/p/micro-flow-imaging-mfi/mfi-5000-series_4000-011-001" > <h3 class="card_title_htag"> <div><h3>MFI 5000</h3></div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>Detect sub-visible particles</p></div> </div> <div class="card-bottom-link"> <div> <a href="/p/micro-flow-imaging-mfi/mfi-5000-series_4000-011-001" > Meet MFI </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/search?keywords=MFI&view=condensed&category_attr=Micro-flow%20Imaging%20%28MFI%29" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: ; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/instruments/proteinsimple-consumable-MFI-230x210.png" alt="MFI consumables by ProteinSimple a Bio-Techne brand" loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="/search?keywords=MFI&view=condensed&category_attr=Micro-flow%20Imaging%20%28MFI%29" > <h3 class="card_title_htag"> <div><h3>MFI Consumables</h3></div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>You can now order consumables for MFI online</p></div> </div> <div class="card-bottom-link"> <div> <a href="/search?keywords=MFI&view=condensed&category_attr=Micro-flow%20Imaging%20%28MFI%29" > Shop MFI Consumables </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="https://info.proteinsimple.com/Micro-Flow-Imaging-Virtual-Demo-Experience.html" > <div class="teaser-card-image-crop mb-3"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/instruments/teaser-mfi-virtual-demo-525x315.png" alt="MFI Virtual Demo" loading="lazy" > </div> </a> <a class="card_title_link" href="https://info.proteinsimple.com/Micro-Flow-Imaging-Virtual-Demo-Experience.html" > <h3 class="card_title_htag"> <div><h3>MFI Virtual Demo</h3></div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>See how MFI easily detects, quantitates, and provides morphological data on subvisible particles</p></div> </div> <div class="card-bottom-link"> <div> <a href="https://info.proteinsimple.com/Micro-Flow-Imaging-Virtual-Demo-Experience.html" > Explore MFI </a> </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--individual-button mb-4 paragraph--view-mode--default w-100"> <div class="row"> <div class="col"> <a href="/p/instruments/micro-flow-imaging/request-quote" class="btn btn-primary" type="link" > Request MFI Pricing </a> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><a name="how"></a></p></div> </div> </div> <div> <div class="paragraph paragraph--type--_-2-text-to-media mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_header_2_global"> <div><h2>How does MFI work?</h2></div> </div> <div class="field_block:paragraph:2_2_text_to_media:field_lg_sngl_frmtd_text_global"> <div><p>Micro-Flow Imaging combines the direct imaging capabilities of digital microscopy with the precise control of microfluidics. What does that get you? High-resolution images with 85% sampling efficiency, more precise counts and sizing with full morphological detail for all sub-visible particles in your sample and the complete confidence that you can accurately identify every possible type, from protein aggregates to air bubbles.</p> <p>Images of the sample are captured as it passes through the flow cell's sensing zone. Every particle in every image is then analyzed to create a database of particle count, size, transparency and morphology (or shape). And you'll have visual verification on the spot, as images are displayed in real-time. You can also display the results for many samples at once, which makes it easy to monitor stability and comparability. </p></div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/instruments/mfi-diagram.jpg" alt="MFI Diagram of the Micro-Flow Imaging Process Including Digital Camera, Optics, Samples, and Flow Cell" loading="lazy" > </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><h3><a name="comparison"></a>MFI Systems Comparison Chart</h3> <figure class="responsive-figure-table"><table class="table table-bordered"> <thead> <tr> <th> <p><strong>Description</strong></p> </th> <th> <p><strong>MFI 5100</strong></p> </th> <th> <p><strong>MFI 5200</strong></p> </th> </tr> </thead> <tbody> <tr> <td> <p><strong>Size Range</strong></p> </td> <td> <p>2 to 300 µm</p> </td> <td> <p>1 to 70 µm</p> </td> </tr> <tr> <td> <p><strong>Percent of Sample Analyzed</strong></p> </td> <td> <p>>85% - entire size range</p> </td> <td> <p>>85% - entire size range</p> </td> </tr> <tr> <td> <p><strong>Depth of Field (DOF)</strong></p> </td> <td> <p>400 µm</p> </td> <td> <p>100 µm</p> </td> </tr> <tr> <td> <p><strong>Flow Cell Depth</strong></p> </td> <td> <p>400 µm - DOF matched</p> </td> <td> <p>100 µm - DOF matched</p> </td> </tr> <tr> <td> <p><strong>Analysis Rate</strong></p> </td> <td> <p>200 µL/min</p> </td> <td> <p>150 µL/min</p> </td> </tr> <tr> <td> <p><strong>Maximum Concentration (@2.5 µm)</strong></p> </td> <td> <p>175,000 particles/mL</p> </td> <td> <p>900,000 particles/mL</p> </td> </tr> </tbody> </table></figure></div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><a name="applications"></a></p></div> </div> </div> <div> <div class="paragraph paragraph--type--card-paragraph paragraph--view-mode--default card-para-default"> <h2>MFI Applications</h2> <div class="row card_row"> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/methods/bioprocessing" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: ; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/applications/application-teaser-bioprocessing-3.jpg" alt="Bioprocessing" loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="/methods/bioprocessing" > <h3 class="card_title_htag"> <div>Bioprocessing</div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>Enhance the safety and efficacy of your formulation and final product by detecting, quantifying, and classifying different sub-visible particles such as protein aggregates, silicone oil, and other contaminants.</p></div> </div> <div class="card-bottom-link"> <div> <a href="/methods/bioprocessing" > Innovations in Bioprocess Workflow Solutions </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/resources/instrument-applications/vector-characterization#maurice" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: ; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/applications/teaser-cellgenetherapy-2.jpg" alt="Cell & Gene Therapy" loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="/resources/instrument-applications/vector-characterization#maurice" > <h3 class="card_title_htag"> <div>Cell & Gene Therapy</div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>Next-generation analytical instrumentation to help improve and accelerate your cell and gene therapy workflows.</p></div> </div> <div class="card-bottom-link"> <div> <a href="/resources/instrument-applications/vector-characterization#maurice" > Tools for Cell & Gene Therapy </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/p/micro-flow-imaging-mfi/mfi-5000-series_4000-011-001" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: ; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/applications/application2-teaser-proteinaggregation-2.jpg" alt="Protein Aggregation and Particle Analysis" loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="/p/micro-flow-imaging-mfi/mfi-5000-series_4000-011-001" > <h3 class="card_title_htag"> <div>Protein Aggregation & Particle Analysis</div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>Improve the safety of your therapeutic with accurate detection and characterization of translucent protein aggregates and other particulates.</p></div> </div> <div class="card-bottom-link"> <div> <a href="/p/micro-flow-imaging-mfi/mfi-5000-series_4000-011-001" > Particulate Analysis for Safer Therapeutics </a> </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--card-paragraph paragraph--view-mode--default card-para-default"> <div class="row card_row"> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/resources/instrument-applications/let-bio-techne-help-you-get-your-biosimilars-to-market-mfi-ice" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: ; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/applications/application-biosimilars-2.jpg" alt="Biosimilars" loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="/resources/instrument-applications/let-bio-techne-help-you-get-your-biosimilars-to-market-mfi-ice" > <h3 class="card_title_htag"> <div>Biosimilars</div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>Be assured of your biosimilar’s stability through the reproducible detection and quantitation of particulate matter and aggregates during development, formulation, and manufacturing.</p></div> </div> <div class="card-bottom-link"> <div> <a href="/resources/instrument-applications/let-bio-techne-help-you-get-your-biosimilars-to-market-mfi-ice" > Tools for Biosimilar Development </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/resources/instrument-applications/let-maurice-and-mfi-characterize-your-vaccine-products" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: ; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/applications/teaser-vaccinedevelopment-2.jpg" alt="Vaccine" loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="/resources/instrument-applications/let-maurice-and-mfi-characterize-your-vaccine-products" > <h3 class="card_title_htag"> <div>Vaccine Development</div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>Develop and manufacture safe vaccines by accurately visualizing and classifying different particle contaminants in your product, from development to delivery.</p></div> </div> <div class="card-bottom-link"> <div> <a href="/resources/instrument-applications/let-maurice-and-mfi-characterize-your-vaccine-products" > Accelerate your Vaccine Development </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--button-row paragraph--view-mode--default"> <div class="row"> <div class="col"> <div><h3>Additional Resources</h3></div> <div class="list-grid-wrap"> <div class="row"> <div class="col-md-4"> <a href="/GMP-Capabilities" class="btn btn-primary" type="link"> GMP Capabilities </a> </div> <div class="col-md-4"> <a href="/reagents/cell-culture-reagents" class="btn btn-primary" type="link"> Cell Culture Reagents </a> </div> <div class="col-md-4"> <a href="/resources/literature/micro-flow-imaging-brochure" class="btn btn-primary" type="link"> Download MFI Brochure </a> </div> </div> </div> </div> </div> </div> </div> </div> </div> </article> </div> </div> </section> 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