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What is Hybridoma? - Creative Biolabs

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Based on our extensive experience and advanced platform, we are proud to offer hybridoma services with different species for our customers all over the world. </p> <h3> Introduction of Hybridoma Technology </h3> <p> Hybridoma technology is a traditional method for the long-term production of large-scale mAb. Derived from the fusion of specific antibody-producing B lymphoblast with myeloma, the forming of the hybrid cell line is the key part of hybridoma technology. The general development of hybridoma cells starts with animal immunization and then the splenocytes would be harvested. The B cells that produce specific antibodies are isolated and in turn fused with myeloma. The obtained stable fusion cells are known as hybridoma cells, which can express antigen-specific mAb continuously. MAbs are of great value not only in basic research of biology and immunology but also in medical applications. In addition, equipped with other advanced techniques available in Creative Biolabs, such as antibody-drug conjugates (ADCs), <a href="/antibody-sequencing-service.html">hybridoma sequencing</a>, and bioconjugation the extended application of mAbs can further assist the development of either therapeutic or diagnostic agents. </p> <p class="ServiceShowPic"> <img src="static/img/Hybridoma-Services-1.png" width="600" alt="Hybridoma technology. (Saeed, et al., 2017)" />Fig.1 Hybridoma technology.<sup>1</sup> </p> <h3> One-stop MAb Production by Hybridoma </h3> <ul class="ullist disc"> <li> Antigen Preparation </li> </ul> <p> Our scientists have extensive experience in handling various types of antigen. We can work with the purified antigen or start with the provided in silico sequence. The soluble proteins and peptides can be generated through our advanced recombinant/synthesis system. In terms of the novel <a href="/Membrane-Protein-Production.html">Magic™ membrane protein production services</a>, Creative Biolabs can produce membrane protein with various formats to meet our clients' requirements. Comprehensive <a href="/immunogen-design-service.html">Immunogen Design Services</a> are available for you. </p> <ul class="ullist disc"> <li> Immunization </li> </ul> <p> To meet our clients' specific project demands, we can tailor <a href="/comprehensive-immunization-strategies-for-novel-antibody-discovery.html">comprehensive immunization strategies</a> which including but not limited to <a href="/antibody-production-through-DNA-immunization-genetic-immunization.html">DNA immunization</a>, <a href="/antibody-production-by-whole-cell-immunization.html">whole cell immunization</a>, and other <a href="/antibody-production-by-conventional-immunization.html">conventional immunization</a> strategies. </p> <ul class="ullist disc"> <li> Hybridoma Generation and Screening </li> </ul> <p> Our optimized fusion protocols enable the fusion of splenocytes with proper myeloma cells. Various protocols can be performed for the positive hybridoma screening, including immunoblotting, immunoprecipitation, ELISA, and <a href="/IHC-Positive-Hybridoma-Screening.html">IHC-positive hybridoma screening</a>. </p> <ul class="ullist disc"> <li> Antibody Production and Purification </li> </ul> <p> The antibody preparation (<em>in vivo</em> or <em>in vitro</em>) and purification will be conducted based on customer needs. High-quality antibodies will be delivered. </p> <div class="table-responsive"> <div class="hybridoma-services"> <div class="hunit"> <div class="linkfont"> <a href="function-guaranteed-antibody-development.html">Function-guaranteed Hybridoma Development</a> </div> </div> <div class="hunit"> <div class="linkfont"> <a href="precision-antibody-replication-service.html">Precision Antibody Replication Service</a> </div> </div> <div class="hunit"> <div class="linkfont"> <a href="hybridoma-isotype-identification-and-switching-service.html">Hybridoma Isotype Identification and Switching Service </a> </div> </div> <div class="hunit"> <div class="linkfont"> <a href="hybridoma-rescue-service.html">Hybridoma Rescue Service </a> </div> </div> <div class="hunit"> <div class="linkfont"> <a href="bulk-antibody-production-services.html">Bulk Antibody Production Service </a> </div> </div> <div class="hunit"> <div class="linkfont"> <a href="anti-small-molecules-hybridoma-development.html">Anti-Small Molecules Hybridoma Development </a> </div> </div> </div> </div> <h3> Features of Hybridoma Services from Creative Biolabs </h3> <ul class="ullist disc"> <li> High specificity, high affinity, and high accuracy </li> <li> Diverse species selection (<a href="/monoclonal-antibody-production-rat-mouse.html">mouse hybridoma</a>, <a href="/Rat-Hybridoma-Generation.html">rat hybridoma</a>, <a href="/Hamster-Hybridoma-Production.html">hamster hybridoma</a>, <a href="/Guinea-Pig-Hybridoma-Production.htm">guinea pig hybridoma</a>, and <a href="/Hybridoma-Generation-in-Gene-Inactivated-Mice.html">gene-inactivated mice hybridoma</a>) </li> <li> Enable the recognization of subtle changes (<a href="/Custom-antibody-services-for-post-translational-modification-specific-antibody-discovery.html">post-translational modifications</a>) </li> <li> Generation of large panels of bioactive antibodies </li> <li> Comprehensive functional testing is available </li> <li> Novel epitope recognition </li> <li> Excellent IHC results with formalin-fixed paraffin-embedded tissue </li> <li> Excellent results for difficult antigens </li> <li> High-throughput screening platform available </li> </ul> <br /> <p> <strong>Creative Biolabs</strong> has been a long-term expert in the field of mAb generation. Equipped with our advanced hybridoma platform, we are confident in offering various hybridoma-based services. If you are interested in our services, please do not hesitate to <a class="inquiry-button">inquire</a> us for more details. </p> <p class="Reference"> <strong>Reference</strong> </p> <ol class="ollist Reference"> <li> Saeed, Abdullah FUH, et al. "Antibody engineering for pursuing a healthier future."<em> Frontiers in microbiology</em> (2017): 495. </li> </ol><br> <p class="pronote"><i class="fa fa-exclamation-triangle"></i>All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.</p> <div class="related-sections"> <b>Related Sections</b> <b>Services</b> <ul class="related-sections-ul"> <li><a href="/magic-human-antibody-production-through-hybridoma-technology.html" >Hybridoma based Magic™ Human Antibody Discovery Service</a></li> <li><a href="/Hybridoma-preparation-and-anti-membrane-protein-mAbs-production-service.html" >Anti-Membrane Protein Antibody Discovery Using Hybridoma</a></li> <li><a href="/dna-immunization-based-hybridoma-production-service.html" >DNA Immunization based Hybridoma Production Service</a></li> <li><a href="/phage-display-service.html" >Phage Display based Binder Discovery Services</a></li> <li><a href="/Native-TM-Antibody-Discovery-Service.html" >Single B Cell Sorting based Native™ Antibody Discovery Services</a></li> <li><a href="/Custom-Antibody-Productions.html" >Custom Antibody Discovery Service</a></li> </ul> <b>Platforms</b> <ul class="related-sections-ul"> <li><a 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