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href="https://www.academia.edu/Documents/in/Environmental_microbiology">Environmental microbiology</a>, <script data-card-contents-for-ri="1444" type="text/json">{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="11801" rel="nofollow" href="https://www.academia.edu/Documents/in/Environmental_Monitoring">Environmental Monitoring</a>, <script data-card-contents-for-ri="11801" type="text/json">{"id":11801,"name":"Environmental Monitoring","url":"https://www.academia.edu/Documents/in/Environmental_Monitoring?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="28235" rel="nofollow" href="https://www.academia.edu/Documents/in/Multidisciplinary">Multidisciplinary</a>, <script data-card-contents-for-ri="28235" type="text/json">{"id":28235,"name":"Multidisciplinary","url":"https://www.academia.edu/Documents/in/Multidisciplinary?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="37894" rel="nofollow" href="https://www.academia.edu/Documents/in/Quantitative_PCR">Quantitative PCR</a><script data-card-contents-for-ri="37894" type="text/json">{"id":37894,"name":"Quantitative PCR","url":"https://www.academia.edu/Documents/in/Quantitative_PCR?f_ri=1444","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=6917633]'), work: {"id":6917633,"title":"Evaluation of the Biological Sampling Kit (BiSKit) for Large-Area Surface Sampling","created_at":"2014-04-30T00:40:59.064-07:00","url":"https://www.academia.edu/6917633/Evaluation_of_the_Biological_Sampling_Kit_BiSKit_for_Large_Area_Surface_Sampling?f_ri=1444","dom_id":"work_6917633","summary":null,"downloadable_attachments":[{"id":33595743,"asset_id":6917633,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":11649584,"first_name":"patricia","last_name":"cruz","domain_name":"independent","page_name":"patriciacruz7","display_name":"patricia cruz","profile_url":"https://independent.academia.edu/patriciacruz7?f_ri=1444","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true},{"id":11801,"name":"Environmental Monitoring","url":"https://www.academia.edu/Documents/in/Environmental_Monitoring?f_ri=1444","nofollow":true},{"id":28235,"name":"Multidisciplinary","url":"https://www.academia.edu/Documents/in/Multidisciplinary?f_ri=1444","nofollow":true},{"id":37894,"name":"Quantitative PCR","url":"https://www.academia.edu/Documents/in/Quantitative_PCR?f_ri=1444","nofollow":true},{"id":71578,"name":"Wood","url":"https://www.academia.edu/Documents/in/Wood?f_ri=1444"},{"id":73645,"name":"Sampling methods","url":"https://www.academia.edu/Documents/in/Sampling_methods?f_ri=1444"},{"id":96502,"name":"Applied","url":"https://www.academia.edu/Documents/in/Applied?f_ri=1444"},{"id":107664,"name":"Applied Environmental Microbiology","url":"https://www.academia.edu/Documents/in/Applied_Environmental_Microbiology?f_ri=1444"},{"id":112332,"name":"Bacillus","url":"https://www.academia.edu/Documents/in/Bacillus?f_ri=1444"},{"id":118339,"name":"Polymerase Chain Reaction","url":"https://www.academia.edu/Documents/in/Polymerase_Chain_Reaction?f_ri=1444"},{"id":255058,"name":"Metals","url":"https://www.academia.edu/Documents/in/Metals?f_ri=1444"},{"id":295244,"name":"Indoor air pollution","url":"https://www.academia.edu/Documents/in/Indoor_air_pollution?f_ri=1444"},{"id":386356,"name":"Surface Area","url":"https://www.academia.edu/Documents/in/Surface_Area?f_ri=1444"},{"id":407880,"name":"Sampling strategy","url":"https://www.academia.edu/Documents/in/Sampling_strategy?f_ri=1444"},{"id":440924,"name":"Surface Properties","url":"https://www.academia.edu/Documents/in/Surface_Properties?f_ri=1444"},{"id":511535,"name":"Immunoassay","url":"https://www.academia.edu/Documents/in/Immunoassay?f_ri=1444"},{"id":901876,"name":"Sensitivity and Specificity","url":"https://www.academia.edu/Documents/in/Sensitivity_and_Specificity?f_ri=1444"},{"id":957359,"name":"Culture Media","url":"https://www.academia.edu/Documents/in/Culture_Media?f_ri=1444"},{"id":1005336,"name":"Bacillus Anthracis","url":"https://www.academia.edu/Documents/in/Bacillus_Anthracis?f_ri=1444"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_20944309" data-work_id="20944309" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/20944309/Black_truffle_associated_bacterial_communities_during_the_development_and_maturation_of_T_uber_melanosporum_ascocarps_and_putative_functional_roles">Black truffle - associated bacterial communities during the development and maturation of T uber melanosporum ascocarps and putative functional roles</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Although truffles are cultivated since decades, their life cycle and the conditions stimulating ascocarp formation still remain mysterious. A role for bacteria in the development of several truffle species has been suggested but few is... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_20944309" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Although truffles are cultivated since decades, their life cycle and the conditions stimulating ascocarp formation still remain mysterious. A role for bacteria in the development of several truffle species has been suggested but few is known regarding the natural bacterial communities of Périgord Black truffle. Thus, the aim of this study was to decipher the structure and the functional potential of the bacterial communities associated to the Black truffle in the course of its life cycle and along truffle maturation. A polyphasic approach combining 454-pyrosequencing of 16S rRNA gene, TTGE, in situ hybridization and functional GeoChip 3.0 revealed that Black truffle ascocarps provide a habitat to complex bacterial communities that are clearly differentiated from those of the surrounding soil and the ectomycorrhizosphere. The composition of these communities is dynamic and evolves during the maturation of the ascocarps with an enrichment of specific taxa and a differentiation of the gleba and peridium-associated bacterial communities. Genes related to nitrogen and sulphur cycling were enriched in the ascocarps. Together, these data paint a new picture of the interactions existing between truffle and bacteria and of the potential role of these bacteria in truffle maturation.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/20944309" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="7770dc76d6fa920b8465488842da1e8c" rel="nofollow" data-download="{"attachment_id":41639768,"asset_id":20944309,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/41639768/download_file?st=MTc0MDU3NTMzMiw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="41545887" href="https://un-lincoln.academia.edu/SanjayAntonyBabu">Sanjay Antony-Babu</a><script data-card-contents-for-user="41545887" type="text/json">{"id":41545887,"first_name":"Sanjay","last_name":"Antony-Babu","domain_name":"un-lincoln","page_name":"SanjayAntonyBabu","display_name":"Sanjay Antony-Babu","profile_url":"https://un-lincoln.academia.edu/SanjayAntonyBabu?f_ri=1444","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_20944309 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="20944309"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 20944309, container: ".js-paper-rank-work_20944309", }); 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$(".js-view-count[data-work-id=20944309]").text(description); $(".js-view-count-work_20944309").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_20944309").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="20944309"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">8</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="159" rel="nofollow" href="https://www.academia.edu/Documents/in/Microbiology">Microbiology</a>, <script data-card-contents-for-ri="159" type="text/json">{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="1444" rel="nofollow" href="https://www.academia.edu/Documents/in/Environmental_microbiology">Environmental microbiology</a>, <script data-card-contents-for-ri="1444" type="text/json">{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="16646" rel="nofollow" href="https://www.academia.edu/Documents/in/Microbial_consortia">Microbial consortia</a>, <script data-card-contents-for-ri="16646" type="text/json">{"id":16646,"name":"Microbial consortia","url":"https://www.academia.edu/Documents/in/Microbial_consortia?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="113903" rel="nofollow" href="https://www.academia.edu/Documents/in/Bacteria">Bacteria</a><script data-card-contents-for-ri="113903" type="text/json">{"id":113903,"name":"Bacteria","url":"https://www.academia.edu/Documents/in/Bacteria?f_ri=1444","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=20944309]'), work: {"id":20944309,"title":"Black truffle - associated bacterial communities during the development and maturation of T uber melanosporum ascocarps and putative functional roles","created_at":"2016-01-27T07:03:45.135-08:00","url":"https://www.academia.edu/20944309/Black_truffle_associated_bacterial_communities_during_the_development_and_maturation_of_T_uber_melanosporum_ascocarps_and_putative_functional_roles?f_ri=1444","dom_id":"work_20944309","summary":"Although truffles are cultivated since decades, their life cycle and the conditions stimulating ascocarp formation still remain mysterious. A role for bacteria in the development of several truffle species has been suggested but few is known regarding the natural bacterial communities of Périgord Black truffle. Thus, the aim of this study was to decipher the structure and the functional potential of the bacterial communities associated to the Black truffle in the course of its life cycle and along truffle maturation. A polyphasic approach combining 454-pyrosequencing of 16S rRNA gene, TTGE, in situ hybridization and functional GeoChip 3.0 revealed that Black truffle ascocarps provide a habitat to complex bacterial communities that are clearly differentiated from those of the surrounding soil and the ectomycorrhizosphere. The composition of these communities is dynamic and evolves during the maturation of the ascocarps with an enrichment of specific taxa and a differentiation of the gleba and peridium-associated bacterial communities. Genes related to nitrogen and sulphur cycling were enriched in the ascocarps. Together, these data paint a new picture of the interactions existing between truffle and bacteria and of the potential role of these bacteria in truffle maturation.","downloadable_attachments":[{"id":41639768,"asset_id":20944309,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":41545887,"first_name":"Sanjay","last_name":"Antony-Babu","domain_name":"un-lincoln","page_name":"SanjayAntonyBabu","display_name":"Sanjay Antony-Babu","profile_url":"https://un-lincoln.academia.edu/SanjayAntonyBabu?f_ri=1444","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=1444","nofollow":true},{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true},{"id":16646,"name":"Microbial consortia","url":"https://www.academia.edu/Documents/in/Microbial_consortia?f_ri=1444","nofollow":true},{"id":113903,"name":"Bacteria","url":"https://www.academia.edu/Documents/in/Bacteria?f_ri=1444","nofollow":true},{"id":231547,"name":"Soil Microbiology","url":"https://www.academia.edu/Documents/in/Soil_Microbiology?f_ri=1444"},{"id":231549,"name":"Rhizosphere","url":"https://www.academia.edu/Documents/in/Rhizosphere?f_ri=1444"},{"id":373754,"name":"Ecosystem","url":"https://www.academia.edu/Documents/in/Ecosystem?f_ri=1444"},{"id":994106,"name":"Ascomycota","url":"https://www.academia.edu/Documents/in/Ascomycota?f_ri=1444"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_73933243" data-work_id="73933243" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/73933243/Indoor_fungal_diversity_in_primary_schools_may_differently_influence_allergic_sensitization_and_asthma_in_children">Indoor fungal diversity in primary schools may differently influence allergic sensitization and asthma in children</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Childhood exposure to microbiological agents may influence the development of allergic and respiratory diseases. Apart from home, children spend most of their time at school, which represents an environment of significant exposure to... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_73933243" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Childhood exposure to microbiological agents may influence the development of allergic and respiratory diseases. Apart from home, children spend most of their time at school, which represents an environment of significant exposure to indoor air microbes. Therefore, we aimed to assess how the prevalence of allergic sensitization and asthma in schoolchildren is affected by microbiological exposure within classrooms. Spirometry with bronchodilation, exhaled nitric oxide measurements and skin-prick tests data were retrieved from 858 children aged 8 to 10 years old attending 71 classrooms in 20 primary schools. Air samples were collected in all classrooms using a single-stage microbiological air impactor through agar plates. Gram-negative endotoxins were collected using flow control pumps and analysed by limulus amebocyte lysate assay. Diversity scores were established as the number of different fungal species found in each classroom. Classrooms with increased diversity scores showed a s...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/73933243" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="af79f973511e5d80775b8916f52b5928" rel="nofollow" data-download="{"attachment_id":82269853,"asset_id":73933243,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/82269853/download_file?st=MTc0MDU3NTMzMiw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="56242989" href="https://independent.academia.edu/Patr%C3%ADciaPadr%C3%A3o">Patrícia Padrão</a><script data-card-contents-for-user="56242989" type="text/json">{"id":56242989,"first_name":"Patrícia","last_name":"Padrão","domain_name":"independent","page_name":"PatríciaPadrão","display_name":"Patrícia Padrão","profile_url":"https://independent.academia.edu/Patr%C3%ADciaPadr%C3%A3o?f_ri=1444","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_73933243 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="73933243"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 73933243, container: ".js-paper-rank-work_73933243", }); });</script></li><li class="js-percentile-work_73933243 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 73933243; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_73933243"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_73933243 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="73933243"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 73933243; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=73933243]").text(description); $(".js-view-count-work_73933243").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_73933243").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="73933243"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">17</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="1290" rel="nofollow" href="https://www.academia.edu/Documents/in/Immunology">Immunology</a>, <script data-card-contents-for-ri="1290" type="text/json">{"id":1290,"name":"Immunology","url":"https://www.academia.edu/Documents/in/Immunology?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="1444" rel="nofollow" href="https://www.academia.edu/Documents/in/Environmental_microbiology">Environmental microbiology</a>, <script data-card-contents-for-ri="1444" type="text/json">{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="9968" rel="nofollow" href="https://www.academia.edu/Documents/in/Asthma">Asthma</a>, <script data-card-contents-for-ri="9968" type="text/json">{"id":9968,"name":"Asthma","url":"https://www.academia.edu/Documents/in/Asthma?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="14911" rel="nofollow" href="https://www.academia.edu/Documents/in/Asthma_In_Children">Asthma In Children</a><script data-card-contents-for-ri="14911" type="text/json">{"id":14911,"name":"Asthma In Children","url":"https://www.academia.edu/Documents/in/Asthma_In_Children?f_ri=1444","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=73933243]'), work: {"id":73933243,"title":"Indoor fungal diversity in primary schools may differently influence allergic sensitization and asthma in children","created_at":"2022-03-17T01:51:06.114-07:00","url":"https://www.academia.edu/73933243/Indoor_fungal_diversity_in_primary_schools_may_differently_influence_allergic_sensitization_and_asthma_in_children?f_ri=1444","dom_id":"work_73933243","summary":"Childhood exposure to microbiological agents may influence the development of allergic and respiratory diseases. Apart from home, children spend most of their time at school, which represents an environment of significant exposure to indoor air microbes. Therefore, we aimed to assess how the prevalence of allergic sensitization and asthma in schoolchildren is affected by microbiological exposure within classrooms. Spirometry with bronchodilation, exhaled nitric oxide measurements and skin-prick tests data were retrieved from 858 children aged 8 to 10 years old attending 71 classrooms in 20 primary schools. Air samples were collected in all classrooms using a single-stage microbiological air impactor through agar plates. Gram-negative endotoxins were collected using flow control pumps and analysed by limulus amebocyte lysate assay. Diversity scores were established as the number of different fungal species found in each classroom. Classrooms with increased diversity scores showed a s...","downloadable_attachments":[{"id":82269853,"asset_id":73933243,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":56242989,"first_name":"Patrícia","last_name":"Padrão","domain_name":"independent","page_name":"PatríciaPadrão","display_name":"Patrícia Padrão","profile_url":"https://independent.academia.edu/Patr%C3%ADciaPadr%C3%A3o?f_ri=1444","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":1290,"name":"Immunology","url":"https://www.academia.edu/Documents/in/Immunology?f_ri=1444","nofollow":true},{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true},{"id":9968,"name":"Asthma","url":"https://www.academia.edu/Documents/in/Asthma?f_ri=1444","nofollow":true},{"id":14911,"name":"Asthma In Children","url":"https://www.academia.edu/Documents/in/Asthma_In_Children?f_ri=1444","nofollow":true},{"id":17825,"name":"Biodiversity","url":"https://www.academia.edu/Documents/in/Biodiversity?f_ri=1444"},{"id":20212,"name":"Allergic asthma","url":"https://www.academia.edu/Documents/in/Allergic_asthma?f_ri=1444"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine?f_ri=1444"},{"id":44510,"name":"Portugal","url":"https://www.academia.edu/Documents/in/Portugal?f_ri=1444"},{"id":53948,"name":"Human Microbiome","url":"https://www.academia.edu/Documents/in/Human_Microbiome?f_ri=1444"},{"id":62896,"name":"Allergy","url":"https://www.academia.edu/Documents/in/Allergy?f_ri=1444"},{"id":64933,"name":"Child","url":"https://www.academia.edu/Documents/in/Child?f_ri=1444"},{"id":110914,"name":"Childhood asthma","url":"https://www.academia.edu/Documents/in/Childhood_asthma?f_ri=1444"},{"id":168977,"name":"Allergens","url":"https://www.academia.edu/Documents/in/Allergens?f_ri=1444"},{"id":187637,"name":"Bronchial Asthma Control in General Practice","url":"https://www.academia.edu/Documents/in/Bronchial_Asthma_Control_in_General_Practice?f_ri=1444"},{"id":320544,"name":"Penicillium","url":"https://www.academia.edu/Documents/in/Penicillium?f_ri=1444"},{"id":2578363,"name":"Mycoses","url":"https://www.academia.edu/Documents/in/Mycoses?f_ri=1444"},{"id":3789883,"name":"Paediatrics and reproductive medicine","url":"https://www.academia.edu/Documents/in/Paediatrics_and_reproductive_medicine?f_ri=1444"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_4498224" data-work_id="4498224" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/4498224/Comparative_analysis_of_antibiotic_resistance_immunofluorescent_colony_staining_and_a_transgenic_marker_bioluminescence_for_monitoring_the_environmental_fate_of_a_Rhizobacterium">Comparative analysis of antibiotic resistance, immunofluorescent colony staining, and a transgenic marker (bioluminescence) for monitoring the environmental fate of a Rhizobacterium</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Mycoplasma genitalium has been proposed as a suitable model for an in-depth understanding of the biology of a free-living organism. This paper reports that the expression of the aminoglycoside resistance gene aac(69)-aph(20), the only... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_4498224" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Mycoplasma genitalium has been proposed as a suitable model for an in-depth understanding of the biology of a free-living organism. This paper reports that the expression of the aminoglycoside resistance gene aac(69)-aph(20), the only selectable marker hitherto available for M. genitalium genetic studies, correlates with a growth impairment of the resistant strains. In light of this finding, a tetM438 construction based on the tetracycline resistance gene tetM was developed; it can be used efficiently in M. genitalium and confers multiple advantages when compared to aac(69)-aph(20). The use of tetM438 significantly improves transformation efficiency and generates visible colonies faster. Finally, the improvements in the pMTnTetM438 construction made it possible to obtain insertions in genes which have not been previously considered to be dispensable under laboratory growth conditions.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/4498224" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="bd06aff166741b37e67fc00144fda902" rel="nofollow" data-download="{"attachment_id":49821506,"asset_id":4498224,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/49821506/download_file?st=MTc0MDU3NTMzMyw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="5577513" href="https://usda-ars.academia.edu/WalterMahaffee">Walter Mahaffee</a><script data-card-contents-for-user="5577513" type="text/json">{"id":5577513,"first_name":"Walter","last_name":"Mahaffee","domain_name":"usda-ars","page_name":"WalterMahaffee","display_name":"Walter Mahaffee","profile_url":"https://usda-ars.academia.edu/WalterMahaffee?f_ri=1444","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_4498224 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="4498224"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 4498224, container: ".js-paper-rank-work_4498224", }); 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$(".js-view-count[data-work-id=4498224]").text(description); $(".js-view-count-work_4498224").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_4498224").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="4498224"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">15</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="159" rel="nofollow" href="https://www.academia.edu/Documents/in/Microbiology">Microbiology</a>, <script data-card-contents-for-ri="159" type="text/json">{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="1444" rel="nofollow" href="https://www.academia.edu/Documents/in/Environmental_microbiology">Environmental microbiology</a>, <script data-card-contents-for-ri="1444" type="text/json">{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="6947" rel="nofollow" href="https://www.academia.edu/Documents/in/Medical_Microbiology">Medical Microbiology</a>, <script data-card-contents-for-ri="6947" type="text/json">{"id":6947,"name":"Medical Microbiology","url":"https://www.academia.edu/Documents/in/Medical_Microbiology?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="17960" rel="nofollow" href="https://www.academia.edu/Documents/in/Infectious_Diseases">Infectious Diseases</a><script data-card-contents-for-ri="17960" type="text/json">{"id":17960,"name":"Infectious Diseases","url":"https://www.academia.edu/Documents/in/Infectious_Diseases?f_ri=1444","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=4498224]'), work: {"id":4498224,"title":"Comparative analysis of antibiotic resistance, immunofluorescent colony staining, and a transgenic marker (bioluminescence) for monitoring the environmental fate of a Rhizobacterium","created_at":"2013-09-16T02:14:48.324-07:00","url":"https://www.academia.edu/4498224/Comparative_analysis_of_antibiotic_resistance_immunofluorescent_colony_staining_and_a_transgenic_marker_bioluminescence_for_monitoring_the_environmental_fate_of_a_Rhizobacterium?f_ri=1444","dom_id":"work_4498224","summary":"Mycoplasma genitalium has been proposed as a suitable model for an in-depth understanding of the biology of a free-living organism. This paper reports that the expression of the aminoglycoside resistance gene aac(69)-aph(20), the only selectable marker hitherto available for M. genitalium genetic studies, correlates with a growth impairment of the resistant strains. In light of this finding, a tetM438 construction based on the tetracycline resistance gene tetM was developed; it can be used efficiently in M. genitalium and confers multiple advantages when compared to aac(69)-aph(20). The use of tetM438 significantly improves transformation efficiency and generates visible colonies faster. Finally, the improvements in the pMTnTetM438 construction made it possible to obtain insertions in genes which have not been previously considered to be dispensable under laboratory growth conditions.","downloadable_attachments":[{"id":49821506,"asset_id":4498224,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":5577513,"first_name":"Walter","last_name":"Mahaffee","domain_name":"usda-ars","page_name":"WalterMahaffee","display_name":"Walter Mahaffee","profile_url":"https://usda-ars.academia.edu/WalterMahaffee?f_ri=1444","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=1444","nofollow":true},{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true},{"id":6947,"name":"Medical Microbiology","url":"https://www.academia.edu/Documents/in/Medical_Microbiology?f_ri=1444","nofollow":true},{"id":17960,"name":"Infectious Diseases","url":"https://www.academia.edu/Documents/in/Infectious_Diseases?f_ri=1444","nofollow":true},{"id":28235,"name":"Multidisciplinary","url":"https://www.academia.edu/Documents/in/Multidisciplinary?f_ri=1444"},{"id":35620,"name":"Antibiotic Resistance","url":"https://www.academia.edu/Documents/in/Antibiotic_Resistance?f_ri=1444"},{"id":107664,"name":"Applied Environmental Microbiology","url":"https://www.academia.edu/Documents/in/Applied_Environmental_Microbiology?f_ri=1444"},{"id":115151,"name":"Microbial genetic and drug resistance","url":"https://www.academia.edu/Documents/in/Microbial_genetic_and_drug_resistance?f_ri=1444"},{"id":152553,"name":"Comparative Analysis","url":"https://www.academia.edu/Documents/in/Comparative_Analysis?f_ri=1444"},{"id":158914,"name":"Luminescence","url":"https://www.academia.edu/Documents/in/Luminescence?f_ri=1444"},{"id":313005,"name":"Mycoplasma Genitalium","url":"https://www.academia.edu/Documents/in/Mycoplasma_Genitalium?f_ri=1444"},{"id":323597,"name":"Fluorescent Antibody Technique","url":"https://www.academia.edu/Documents/in/Fluorescent_Antibody_Technique?f_ri=1444"},{"id":335984,"name":"Anti-Bacterial Agents","url":"https://www.academia.edu/Documents/in/Anti-Bacterial_Agents?f_ri=1444"},{"id":858129,"name":"Rhizobiaceae","url":"https://www.academia.edu/Documents/in/Rhizobiaceae?f_ri=1444"},{"id":1232430,"name":"Genetic Markers","url":"https://www.academia.edu/Documents/in/Genetic_Markers?f_ri=1444"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_10690830" data-work_id="10690830" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" rel="nofollow" href="https://www.academia.edu/10690830/Isolation_and_physiological_characterization_of_psychrophilic_denitrifying_bacteria_from_permanently_cold_Arctic_fjord_sediments_Svalbard_Norway_">Isolation and physiological characterization of psychrophilic denitrifying bacteria from permanently cold Arctic fjord sediments (Svalbard, Norway)</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/10690830" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="bdf4b73bc3cc61cf933cf85849f2b941" rel="nofollow" data-download="{"attachment_id":47201881,"asset_id":10690830,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/47201881/download_file?st=MTc0MDU3NTMzMyw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" 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class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/14000472/The_evaluation_of_a_PCR_based_method_for_identification_of_Salmonella_enterica_serotypes_from_environmental_samples_and_various_food_matrices">The evaluation of a PCR-based method for identification of Salmonella enterica serotypes from environmental samples and various food matrices</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">The most commonly used method for serotyping Salmonella spp. is based on the KaufmanneWhite scheme, and is composed of serological reactions using antibodies to LPS agglutinins. The multiplex PCR used in this investigation was established... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_14000472" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">The most commonly used method for serotyping Salmonella spp. is based on the KaufmanneWhite scheme, and is composed of serological reactions using antibodies to LPS agglutinins. The multiplex PCR used in this investigation was established by Kim et al. to serotype the 30 most common clinical Salmonella serotypes, as determined by CDC. The PCR assay consists of two five-plex reactions and a single two-plex PCR reaction, based on six genetic loci from Salmonella enterica serotype Typhimurium and four loci from S. enterica serotype Typhi. In this investigation, we further evaluated the method for serotyping Salmonella spp. using a reference collection, environmental samples collected from a Mid-Atlantic region tomato farm study, four food matrices spiked with different Salmonella serotypes and a proficiency test. The PCR assay was first evaluated using DNA isolated from pure cultures of isolates obtained from various clinical and environmental samples, and then DNA isolated from broth cultures of food matrices of "Red round" and Roma tomatoes, Romaine lettuce, green onions and Serrano peppers spiked with serotypes Newport, Typhimurium, Javiana and Saintpaul, respectively. The results showed that the PCR assay correctly serotyped Salmonella spp. from the clinical, environmental, spiked food matrices, and proficiency test samples. These findings are significant because the PCR assay was successful in the identification of Salmonella in the spiked samples in a broth culture containing other non-salmonella organism. This method may be a useful resource for the food safety community.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/14000472" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="28516c870b48d2c1d9d0c38f579eca63" rel="nofollow" data-download="{"attachment_id":44716253,"asset_id":14000472,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/44716253/download_file?st=MTc0MDU3NTMzMyw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="33037287" href="https://independent.academia.edu/HanesDarcy">Darcy Hanes</a><script data-card-contents-for-user="33037287" type="text/json">{"id":33037287,"first_name":"Darcy","last_name":"Hanes","domain_name":"independent","page_name":"HanesDarcy","display_name":"Darcy Hanes","profile_url":"https://independent.academia.edu/HanesDarcy?f_ri=1444","photo":"/images/s65_no_pic.png"}</script></span></span><span class="u-displayInlineBlock InlineList-item-text"> and <span class="u-textDecorationUnderline u-clickable InlineList-item-text js-work-more-authors-14000472">+1</span><div class="hidden js-additional-users-14000472"><div><span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a href="https://independent.academia.edu/DudleyKim">Kim Dudley</a></span></div></div></span><script>(function(){ var popoverSettings = { el: $('.js-work-more-authors-14000472'), placement: 'bottom', hide_delay: 200, html: true, content: function(){ return $('.js-additional-users-14000472').html(); 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The multiplex PCR used in this investigation was established by Kim et al. to serotype the 30 most common clinical Salmonella serotypes, as determined by CDC. The PCR assay consists of two five-plex reactions and a single two-plex PCR reaction, based on six genetic loci from Salmonella enterica serotype Typhimurium and four loci from S. enterica serotype Typhi. In this investigation, we further evaluated the method for serotyping Salmonella spp. using a reference collection, environmental samples collected from a Mid-Atlantic region tomato farm study, four food matrices spiked with different Salmonella serotypes and a proficiency test. The PCR assay was first evaluated using DNA isolated from pure cultures of isolates obtained from various clinical and environmental samples, and then DNA isolated from broth cultures of food matrices of \"Red round\" and Roma tomatoes, Romaine lettuce, green onions and Serrano peppers spiked with serotypes Newport, Typhimurium, Javiana and Saintpaul, respectively. The results showed that the PCR assay correctly serotyped Salmonella spp. from the clinical, environmental, spiked food matrices, and proficiency test samples. These findings are significant because the PCR assay was successful in the identification of Salmonella in the spiked samples in a broth culture containing other non-salmonella organism. This method may be a useful resource for the food safety community.","downloadable_attachments":[{"id":44716253,"asset_id":14000472,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":33037287,"first_name":"Darcy","last_name":"Hanes","domain_name":"independent","page_name":"HanesDarcy","display_name":"Darcy Hanes","profile_url":"https://independent.academia.edu/HanesDarcy?f_ri=1444","photo":"/images/s65_no_pic.png"},{"id":33069319,"first_name":"Kim","last_name":"Dudley","domain_name":"independent","page_name":"DudleyKim","display_name":"Kim Dudley","profile_url":"https://independent.academia.edu/DudleyKim?f_ri=1444","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=1444","nofollow":true},{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true},{"id":14907,"name":"Industrial Biotechnology","url":"https://www.academia.edu/Documents/in/Industrial_Biotechnology?f_ri=1444","nofollow":true},{"id":31632,"name":"Food Microbiology","url":"https://www.academia.edu/Documents/in/Food_Microbiology?f_ri=1444","nofollow":true},{"id":54433,"name":"Phylogeny","url":"https://www.academia.edu/Documents/in/Phylogeny?f_ri=1444"},{"id":118339,"name":"Polymerase Chain Reaction","url":"https://www.academia.edu/Documents/in/Polymerase_Chain_Reaction?f_ri=1444"},{"id":208544,"name":"Vegetables","url":"https://www.academia.edu/Documents/in/Vegetables?f_ri=1444"},{"id":573653,"name":"Food Sciences","url":"https://www.academia.edu/Documents/in/Food_Sciences?f_ri=1444"},{"id":2032368,"name":"Serotyping","url":"https://www.academia.edu/Documents/in/Serotyping?f_ri=1444"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_21040336" data-work_id="21040336" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/21040336/On_microbes_and_art_the_role_of_microbial_communities_in_the_degradation_and_protection_of_cultural_heritage_A_report_on_the_International_Conference_on_Microbiology_and_Conservation_ICMC_1999_Florence_16_19_June_1999">On microbes and art: the role of microbial communities in the degradation and protection of cultural heritage A report on the International Conference on Microbiology and Conservation (ICMC 1999), Florence,16-19 June 1999</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest">Bibliography A.A.V.V. (1999) Of microbes and art. The role of microbial communities in the degradation and protection of cultural heritage. In</div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/21040336" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="41885df92c97eccda2e69422a68168e9" rel="nofollow" data-download="{"attachment_id":41684681,"asset_id":21040336,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/41684681/download_file?st=MTc0MDU3NTMzMyw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="39367444" href="https://unime.academia.edu/ClaraUrzi">Clara Urzi</a><script data-card-contents-for-user="39367444" type="text/json">{"id":39367444,"first_name":"Clara","last_name":"Urzi","domain_name":"unime","page_name":"ClaraUrzi","display_name":"Clara Urzi","profile_url":"https://unime.academia.edu/ClaraUrzi?f_ri=1444","photo":"https://0.academia-photos.com/39367444/13737458/14855001/s65_clara.urzi.jpg"}</script></span></span></li><li class="js-paper-rank-work_21040336 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="21040336"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 21040336, container: ".js-paper-rank-work_21040336", }); });</script></li><li class="js-percentile-work_21040336 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 21040336; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_21040336"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_21040336 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="21040336"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 21040336; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=21040336]").text(description); $(".js-view-count-work_21040336").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_21040336").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="21040336"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">4</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="159" rel="nofollow" href="https://www.academia.edu/Documents/in/Microbiology">Microbiology</a>, <script data-card-contents-for-ri="159" type="text/json">{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="1444" rel="nofollow" href="https://www.academia.edu/Documents/in/Environmental_microbiology">Environmental microbiology</a>, <script data-card-contents-for-ri="1444" type="text/json">{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="2132" rel="nofollow" href="https://www.academia.edu/Documents/in/Cultural_Heritage">Cultural Heritage</a>, <script data-card-contents-for-ri="2132" type="text/json">{"id":2132,"name":"Cultural Heritage","url":"https://www.academia.edu/Documents/in/Cultural_Heritage?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="1627116" rel="nofollow" href="https://www.academia.edu/Documents/in/Microbial_community">Microbial community</a><script data-card-contents-for-ri="1627116" type="text/json">{"id":1627116,"name":"Microbial community","url":"https://www.academia.edu/Documents/in/Microbial_community?f_ri=1444","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=21040336]'), work: {"id":21040336,"title":"On microbes and art: the role of microbial communities in the degradation and protection of cultural heritage A report on the International Conference on Microbiology and Conservation (ICMC 1999), Florence,16-19 June 1999","created_at":"2016-01-28T04:36:04.091-08:00","url":"https://www.academia.edu/21040336/On_microbes_and_art_the_role_of_microbial_communities_in_the_degradation_and_protection_of_cultural_heritage_A_report_on_the_International_Conference_on_Microbiology_and_Conservation_ICMC_1999_Florence_16_19_June_1999?f_ri=1444","dom_id":"work_21040336","summary":"Bibliography A.A.V.V. (1999) Of microbes and art. The role of microbial communities in the degradation and protection of cultural heritage. In","downloadable_attachments":[{"id":41684681,"asset_id":21040336,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":39367444,"first_name":"Clara","last_name":"Urzi","domain_name":"unime","page_name":"ClaraUrzi","display_name":"Clara Urzi","profile_url":"https://unime.academia.edu/ClaraUrzi?f_ri=1444","photo":"https://0.academia-photos.com/39367444/13737458/14855001/s65_clara.urzi.jpg"}],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=1444","nofollow":true},{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true},{"id":2132,"name":"Cultural Heritage","url":"https://www.academia.edu/Documents/in/Cultural_Heritage?f_ri=1444","nofollow":true},{"id":1627116,"name":"Microbial community","url":"https://www.academia.edu/Documents/in/Microbial_community?f_ri=1444","nofollow":true}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_1556288" data-work_id="1556288" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/1556288/Removal_of_nutrients_in_denitrification_system_using_coconut_coir_fibre_for_the_biological_treatment_of_aquaculture_wastewater">Removal of nutrients in denitrification system using coconut coir fibre for the biological treatment of aquaculture wastewater</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Ideal bacterial support medium for fixed film denitrification processes/bioreactors must be inexpensive, durable and possess large surface area with sufficient porosity. The present study has been focussed on removing nitrate nitrogen at... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_1556288" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Ideal bacterial support medium for fixed film denitrification processes/bioreactors must be inexpensive, durable and possess large surface area with sufficient porosity. The present study has been focussed on removing nitrate nitrogen at two different nitrate nitrogen loading rates (60 (NLR I) and 120 (NLR II) mg l-1) from simulated aquaculture wastewater. Coconut coir fibre and a commercially available synthetic reticulated plastic media (Fujino Spirals) were used as packing medium in two independent upflow anaerobic packed bed column reactors. Removal of nitrate nitrogen was studied in correlation with other nutrients (COD, TKN, dissolved orthophosphate). Maximum removal of 97% at NLR-I and 99% at NLR – II of nitrate nitrogen was observed in with either media. Greater consistency in the case of COD removal of upto 81% was observed at NLR II where coconut coir was used as support medium compared to 72% COD removal by Fujino Spirals. The results observed indicate that the organic support medium is just as efficient in nitrate nitrogen removal as conventionally used synthetic support medium. The study is important as it specifically focuses on denitrification of aquaculture wastewater using cheaper organic support medium in anoxic bioreactors for the removal of nitrate nitrogen; which is seldom addressed as a significant problem.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/1556288" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="3308aed0279709ec5fa1af4c9aaa414c" rel="nofollow" data-download="{"attachment_id":30865179,"asset_id":1556288,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/30865179/download_file?st=MTc0MDU3NTMzMyw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="228658" href="https://veltechuniv.academia.edu/VRManoj">VR Manoj</a><script data-card-contents-for-user="228658" type="text/json">{"id":228658,"first_name":"VR","last_name":"Manoj","domain_name":"veltechuniv","page_name":"VRManoj","display_name":"VR Manoj","profile_url":"https://veltechuniv.academia.edu/VRManoj?f_ri=1444","photo":"https://0.academia-photos.com/228658/50851/12384279/s65_vr.manoj.jpg"}</script></span></span></li><li class="js-paper-rank-work_1556288 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="1556288"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 1556288, container: ".js-paper-rank-work_1556288", }); 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$(".js-view-count[data-work-id=1556288]").text(description); $(".js-view-count-work_1556288").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_1556288").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="1556288"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">17</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="48" rel="nofollow" href="https://www.academia.edu/Documents/in/Engineering">Engineering</a>, <script data-card-contents-for-ri="48" type="text/json">{"id":48,"name":"Engineering","url":"https://www.academia.edu/Documents/in/Engineering?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="55" rel="nofollow" href="https://www.academia.edu/Documents/in/Environmental_Engineering">Environmental Engineering</a>, <script data-card-contents-for-ri="55" type="text/json">{"id":55,"name":"Environmental Engineering","url":"https://www.academia.edu/Documents/in/Environmental_Engineering?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="72" rel="nofollow" href="https://www.academia.edu/Documents/in/Chemical_Engineering">Chemical Engineering</a>, <script data-card-contents-for-ri="72" type="text/json">{"id":72,"name":"Chemical Engineering","url":"https://www.academia.edu/Documents/in/Chemical_Engineering?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="1444" rel="nofollow" href="https://www.academia.edu/Documents/in/Environmental_microbiology">Environmental microbiology</a><script data-card-contents-for-ri="1444" type="text/json">{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=1556288]'), work: {"id":1556288,"title":"Removal of nutrients in denitrification system using coconut coir fibre for the biological treatment of aquaculture wastewater","created_at":"2012-05-13T15:29:51.771-07:00","url":"https://www.academia.edu/1556288/Removal_of_nutrients_in_denitrification_system_using_coconut_coir_fibre_for_the_biological_treatment_of_aquaculture_wastewater?f_ri=1444","dom_id":"work_1556288","summary":"Ideal bacterial support medium for fixed film denitrification processes/bioreactors must be inexpensive, durable and possess large surface area with sufficient porosity. The present study has been focussed on removing nitrate nitrogen at two different nitrate nitrogen loading rates (60 (NLR I) and 120 (NLR II) mg l-1) from simulated aquaculture wastewater. Coconut coir fibre and a commercially available synthetic reticulated plastic media (Fujino Spirals) were used as packing medium in two independent upflow anaerobic packed bed column reactors. Removal of nitrate nitrogen was studied in correlation with other nutrients (COD, TKN, dissolved orthophosphate). Maximum removal of 97% at NLR-I and 99% at NLR – II of nitrate nitrogen was observed in with either media. Greater consistency in the case of COD removal of upto 81% was observed at NLR II where coconut coir was used as support medium compared to 72% COD removal by Fujino Spirals. The results observed indicate that the organic support medium is just as efficient in nitrate nitrogen removal as conventionally used synthetic support medium. The study is important as it specifically focuses on denitrification of aquaculture wastewater using cheaper organic support medium in anoxic bioreactors for the removal of nitrate nitrogen; which is seldom addressed as a significant problem.","downloadable_attachments":[{"id":30865179,"asset_id":1556288,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":228658,"first_name":"VR","last_name":"Manoj","domain_name":"veltechuniv","page_name":"VRManoj","display_name":"VR Manoj","profile_url":"https://veltechuniv.academia.edu/VRManoj?f_ri=1444","photo":"https://0.academia-photos.com/228658/50851/12384279/s65_vr.manoj.jpg"}],"research_interests":[{"id":48,"name":"Engineering","url":"https://www.academia.edu/Documents/in/Engineering?f_ri=1444","nofollow":true},{"id":55,"name":"Environmental Engineering","url":"https://www.academia.edu/Documents/in/Environmental_Engineering?f_ri=1444","nofollow":true},{"id":72,"name":"Chemical Engineering","url":"https://www.academia.edu/Documents/in/Chemical_Engineering?f_ri=1444","nofollow":true},{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true},{"id":3964,"name":"Biochemical Engineering","url":"https://www.academia.edu/Documents/in/Biochemical_Engineering?f_ri=1444"},{"id":4523,"name":"Water quality","url":"https://www.academia.edu/Documents/in/Water_quality?f_ri=1444"},{"id":5413,"name":"Bioenergy","url":"https://www.academia.edu/Documents/in/Bioenergy?f_ri=1444"},{"id":9990,"name":"Water Treatment","url":"https://www.academia.edu/Documents/in/Water_Treatment?f_ri=1444"},{"id":9991,"name":"Wastewater Treatment","url":"https://www.academia.edu/Documents/in/Wastewater_Treatment?f_ri=1444"},{"id":18845,"name":"Environmental Sustainability","url":"https://www.academia.edu/Documents/in/Environmental_Sustainability?f_ri=1444"},{"id":20807,"name":"Nitrogen Cycle","url":"https://www.academia.edu/Documents/in/Nitrogen_Cycle?f_ri=1444"},{"id":23848,"name":"Aquaculture","url":"https://www.academia.edu/Documents/in/Aquaculture?f_ri=1444"},{"id":54182,"name":"Biofuels","url":"https://www.academia.edu/Documents/in/Biofuels?f_ri=1444"},{"id":112950,"name":"Denitrification","url":"https://www.academia.edu/Documents/in/Denitrification?f_ri=1444"},{"id":377915,"name":"Bioprocessing","url":"https://www.academia.edu/Documents/in/Bioprocessing?f_ri=1444"},{"id":931644,"name":"Bioproducts","url":"https://www.academia.edu/Documents/in/Bioproducts?f_ri=1444"},{"id":931645,"name":"Biomass Processing","url":"https://www.academia.edu/Documents/in/Biomass_Processing?f_ri=1444"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_15002923 coauthored" data-work_id="15002923" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/15002923/How_clean_is_clean_is_a_new_microbiology_standard_required">How clean is clean--is a new microbiology standard required?</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">The role of environment in the spread of nosocomial infection has been acknowledged. One way to control the spread of infection is to control and monitor patient environments to prevent transmission. Studies applying the suggested aerobic... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_15002923" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">The role of environment in the spread of nosocomial infection has been acknowledged. One way to control the spread of infection is to control and monitor patient environments to prevent transmission. Studies applying the suggested aerobic colony count standards to monitor environmental contamination were undertaken over an 18-month period at both a London pediatric hospital and in adult intensive care units. The resulting data demonstrate that a large proportion of sites screened for bacterial contamination would fail if using the criteria suggested by previous authorsdparticularly those sites closest to patientsdsuggesting a new standard might be required.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/15002923" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="b3de9dc9bb3b223f205d7ca65cafcab3" rel="nofollow" data-download="{"attachment_id":43662703,"asset_id":15002923,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/43662703/download_file?st=MTc0MDU3NTMzMyw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="34005764" href="https://independent.academia.edu/NikkiDArcy">Nikki D'Arcy</a><script data-card-contents-for-user="34005764" type="text/json">{"id":34005764,"first_name":"Nikki","last_name":"D'Arcy","domain_name":"independent","page_name":"NikkiDArcy","display_name":"Nikki D'Arcy","profile_url":"https://independent.academia.edu/NikkiDArcy?f_ri=1444","photo":"/images/s65_no_pic.png"}</script></span></span><span class="u-displayInlineBlock InlineList-item-text"> and <span class="u-textDecorationUnderline u-clickable InlineList-item-text js-work-more-authors-15002923">+1</span><div class="hidden js-additional-users-15002923"><div><span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a href="https://independent.academia.edu/ElaineCloutmangreen">Elaine Cloutman-green</a></span></div></div></span><script>(function(){ var popoverSettings = { el: $('.js-work-more-authors-15002923'), placement: 'bottom', hide_delay: 200, html: true, content: function(){ return $('.js-additional-users-15002923').html(); 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One way to control the spread of infection is to control and monitor patient environments to prevent transmission. Studies applying the suggested aerobic colony count standards to monitor environmental contamination were undertaken over an 18-month period at both a London pediatric hospital and in adult intensive care units. The resulting data demonstrate that a large proportion of sites screened for bacterial contamination would fail if using the criteria suggested by previous authorsdparticularly those sites closest to patientsdsuggesting a new standard might be required.","downloadable_attachments":[{"id":43662703,"asset_id":15002923,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":34005764,"first_name":"Nikki","last_name":"D'Arcy","domain_name":"independent","page_name":"NikkiDArcy","display_name":"Nikki D'Arcy","profile_url":"https://independent.academia.edu/NikkiDArcy?f_ri=1444","photo":"/images/s65_no_pic.png"},{"id":34099977,"first_name":"Elaine","last_name":"Cloutman-green","domain_name":"independent","page_name":"ElaineCloutmangreen","display_name":"Elaine Cloutman-green","profile_url":"https://independent.academia.edu/ElaineCloutmangreen?f_ri=1444","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":588,"name":"Nursing","url":"https://www.academia.edu/Documents/in/Nursing?f_ri=1444","nofollow":true},{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true},{"id":69637,"name":"Infection Control","url":"https://www.academia.edu/Documents/in/Infection_Control?f_ri=1444","nofollow":true},{"id":410370,"name":"Public health systems and services research","url":"https://www.academia.edu/Documents/in/Public_health_systems_and_services_research-1?f_ri=1444","nofollow":true},{"id":793339,"name":"Intensive Care Units","url":"https://www.academia.edu/Documents/in/Intensive_Care_Units?f_ri=1444"},{"id":1206482,"name":"Cross-infection","url":"https://www.academia.edu/Documents/in/Cross-infection?f_ri=1444"},{"id":1208706,"name":"Environment","url":"https://www.academia.edu/Documents/in/Environment?f_ri=1444"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_77101601" data-work_id="77101601" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/77101601/Identification_of_quorum_sensing_regulated_proteins_in_the_opportunistic_pathogen_Pseudomonas_aeruginosa_by_proteomics">Identification of quorum-sensing regulated proteins in the opportunistic pathogen Pseudomonas aeruginosa by proteomics</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">The Gram-negative bacterium Pseudomonas aeruginosa is an opportunistic human pathogen which is responsible for severe nosocomial infections in immunocompromised patients and is the major pathogen in cystic fibrosis. The bacterium utilizes... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_77101601" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">The Gram-negative bacterium Pseudomonas aeruginosa is an opportunistic human pathogen which is responsible for severe nosocomial infections in immunocompromised patients and is the major pathogen in cystic fibrosis. The bacterium utilizes two interrelated quorum-sensing (QS) systems, which rely on N -acyl-homoserine lactone (AHL) signal molecules, to control the expression of virulence factors and biofilm development. In this study, we compared the protein patterns of the intracellular, extracellular and surface protein fractions of the PAO1 parent strain with those of an isogenic lasI rhlI double mutant by means of two-dimensional gel electrophoresis (2-DE). This analysis showed that the intensities of 23.7% of all detected protein spots differed more than 2.5-fold between the two strains. We only considered those protein spots truly QS regulated that were changed in the mutant in the absence of signal molecules but were rescued to the wild-type situation when the medium was supplemented with AHLs. These protein spots were characterized by MALDI-TOF peptide mapping. Twenty-seven proteins were identified that were previously reported to be AHL controlled, among them several well-characterized virulence factors. For one of the identified proteins, the serine protease PrpL, a biochemical assay was established to verify that expression of this factor is indeed QS regulated. Furthermore, it is shown that the quorum-sensing blocker C-30 specifically interferes with the expression of 67% of the AHLcontrolled protein spots of the surface fraction, confirming the high specificity of the compound. Importantly, 20 novel QS-regulated proteins were identified, many of which are involved in iron utilization, suggesting a link between quorum sensing and the iron regulatory system. Two of these proteins, PhuR and HasAp, are components of the two distinct haem-uptake systems present in P. aeruginosa . In agreement with the finding that both proteins are positively regulated by the QS cascade, we show that the lasI rhlI double mutant grows poorly with haemoglobin as the only iron source when compared with the wild type. These results add haemoglobin utilization to the list of phenotypes controlled through QS in P. aeruginosa . The surprisingly high number of AHLregulated proteins relative to the number of regulated genes suggests that quorum-sensing control also operates via post-transcriptional mechanisms. To strengthen this hypothesis we investigated the role of quorum sensing in the post-translational modification of HasAp, an extracellular protein required for the uptake of free and haemoglobin-bound haem.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/77101601" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="af90d62afca54d92f75d68f9651fc03a" rel="nofollow" data-download="{"attachment_id":84580869,"asset_id":77101601,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/84580869/download_file?st=MTc0MDU3NTMzMyw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="221388749" href="https://independent.academia.edu/MHentzer">Morten Hentzer</a><script data-card-contents-for-user="221388749" type="text/json">{"id":221388749,"first_name":"Morten","last_name":"Hentzer","domain_name":"independent","page_name":"MHentzer","display_name":"Morten Hentzer","profile_url":"https://independent.academia.edu/MHentzer?f_ri=1444","photo":"https://0.academia-photos.com/221388749/79178504/67740444/s65_morten.hentzer.jpeg"}</script></span></span></li><li class="js-paper-rank-work_77101601 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="77101601"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 77101601, container: ".js-paper-rank-work_77101601", }); 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The bacterium utilizes two interrelated quorum-sensing (QS) systems, which rely on N -acyl-homoserine lactone (AHL) signal molecules, to control the expression of virulence factors and biofilm development. In this study, we compared the protein patterns of the intracellular, extracellular and surface protein fractions of the PAO1 parent strain with those of an isogenic lasI rhlI double mutant by means of two-dimensional gel electrophoresis (2-DE). This analysis showed that the intensities of 23.7% of all detected protein spots differed more than 2.5-fold between the two strains. We only considered those protein spots truly QS regulated that were changed in the mutant in the absence of signal molecules but were rescued to the wild-type situation when the medium was supplemented with AHLs. These protein spots were characterized by MALDI-TOF peptide mapping. Twenty-seven proteins were identified that were previously reported to be AHL controlled, among them several well-characterized virulence factors. For one of the identified proteins, the serine protease PrpL, a biochemical assay was established to verify that expression of this factor is indeed QS regulated. Furthermore, it is shown that the quorum-sensing blocker C-30 specifically interferes with the expression of 67% of the AHLcontrolled protein spots of the surface fraction, confirming the high specificity of the compound. Importantly, 20 novel QS-regulated proteins were identified, many of which are involved in iron utilization, suggesting a link between quorum sensing and the iron regulatory system. Two of these proteins, PhuR and HasAp, are components of the two distinct haem-uptake systems present in P. aeruginosa . In agreement with the finding that both proteins are positively regulated by the QS cascade, we show that the lasI rhlI double mutant grows poorly with haemoglobin as the only iron source when compared with the wild type. These results add haemoglobin utilization to the list of phenotypes controlled through QS in P. aeruginosa . The surprisingly high number of AHLregulated proteins relative to the number of regulated genes suggests that quorum-sensing control also operates via post-transcriptional mechanisms. To strengthen this hypothesis we investigated the role of quorum sensing in the post-translational modification of HasAp, an extracellular protein required for the uptake of free and haemoglobin-bound haem.","downloadable_attachments":[{"id":84580869,"asset_id":77101601,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":221388749,"first_name":"Morten","last_name":"Hentzer","domain_name":"independent","page_name":"MHentzer","display_name":"Morten Hentzer","profile_url":"https://independent.academia.edu/MHentzer?f_ri=1444","photo":"https://0.academia-photos.com/221388749/79178504/67740444/s65_morten.hentzer.jpeg"}],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=1444","nofollow":true},{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true},{"id":9786,"name":"Proteomics","url":"https://www.academia.edu/Documents/in/Proteomics?f_ri=1444","nofollow":true},{"id":23323,"name":"Transcription Factors","url":"https://www.academia.edu/Documents/in/Transcription_Factors?f_ri=1444","nofollow":true},{"id":74780,"name":"Mutation","url":"https://www.academia.edu/Documents/in/Mutation?f_ri=1444"},{"id":137610,"name":"Quorum Sensing","url":"https://www.academia.edu/Documents/in/Quorum_Sensing?f_ri=1444"},{"id":158597,"name":"Iron","url":"https://www.academia.edu/Documents/in/Iron?f_ri=1444"},{"id":204375,"name":"Heme","url":"https://www.academia.edu/Documents/in/Heme?f_ri=1444"},{"id":225499,"name":"Pseudomonas aeruginosa","url":"https://www.academia.edu/Documents/in/Pseudomonas_aeruginosa?f_ri=1444"},{"id":599392,"name":"Proteome analysis","url":"https://www.academia.edu/Documents/in/Proteome_analysis?f_ri=1444"},{"id":636395,"name":"Pseudomonas Aeruginosa","url":"https://www.academia.edu/Documents/in/Pseudomonas_Aeruginosa-1?f_ri=1444"},{"id":766010,"name":"Two-Dimensional Gel Electrophoresis","url":"https://www.academia.edu/Documents/in/Two-Dimensional_Gel_Electrophoresis?f_ri=1444"},{"id":923659,"name":"Virulence Factors","url":"https://www.academia.edu/Documents/in/Virulence_Factors?f_ri=1444"},{"id":1032118,"name":"Two dimensional Gel Electrophoresis","url":"https://www.academia.edu/Documents/in/Two_dimensional_Gel_Electrophoresis?f_ri=1444"},{"id":1133132,"name":"Environmental","url":"https://www.academia.edu/Documents/in/Environmental?f_ri=1444"},{"id":1472385,"name":"Butyrolactone","url":"https://www.academia.edu/Documents/in/Butyrolactone?f_ri=1444"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_76142990" data-work_id="76142990" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/76142990/Susceptibility_profile_of_clinical_and_environmental_isolates_ofCryptococcus_neoformansandCryptococcus_gattiiin_Uberaba_Minas_Gerais_Brazil">Susceptibility profile of clinical and environmental isolates ofCryptococcus neoformansandCryptococcus gattiiin Uberaba, Minas Gerais, Brazil</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">formerly considered to be restricted to tropical and subtropical regions, but several outbreaks of cryptococcosis involving both immunocompetent and immunocompromised individuals have been reported in Canada, the US Pacifi c Northwest,... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_76142990" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">formerly considered to be restricted to tropical and subtropical regions, but several outbreaks of cryptococcosis involving both immunocompetent and immunocompromised individuals have been reported in Canada, the US Pacifi c Northwest, Australia, and South America, indicating its geographic expansion [2,3]. In Brazil, C. neoformans is distributed throughout the country, whereas C. gattii is mainly found in the North and Northeast regions [4]. Due to the increase in the number of immunocompromised individuals over the past several decades, the prevalence of invasive fungal infections has substantially increased worldwide [5]. This has led to high rates of therapeutic and/or prophylactic use of azole derivates and other antifungals, causing selective epidemiological pressure, which may have contributed to the decreased susceptibility to these drugs [6].</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/76142990" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="00e7691b58ca42e886903b198ac9eab9" rel="nofollow" data-download="{"attachment_id":83809330,"asset_id":76142990,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/83809330/download_file?st=MTc0MDU3NTMzMyw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="35447045" href="https://ufsb.academia.edu/DelioMora">Delio J Mora</a><script data-card-contents-for-user="35447045" type="text/json">{"id":35447045,"first_name":"Delio","last_name":"Mora","domain_name":"ufsb","page_name":"DelioMora","display_name":"Delio J Mora","profile_url":"https://ufsb.academia.edu/DelioMora?f_ri=1444","photo":"https://0.academia-photos.com/35447045/21349938/20761024/s65_delio.mora.jpg"}</script></span></span></li><li class="js-paper-rank-work_76142990 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="76142990"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 76142990, container: ".js-paper-rank-work_76142990", }); 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$(".js-view-count[data-work-id=76142990]").text(description); $(".js-view-count-work_76142990").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_76142990").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="76142990"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">12</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="159" rel="nofollow" href="https://www.academia.edu/Documents/in/Microbiology">Microbiology</a>, <script data-card-contents-for-ri="159" type="text/json">{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="1444" rel="nofollow" href="https://www.academia.edu/Documents/in/Environmental_microbiology">Environmental microbiology</a>, <script data-card-contents-for-ri="1444" type="text/json">{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="6947" rel="nofollow" href="https://www.academia.edu/Documents/in/Medical_Microbiology">Medical Microbiology</a>, <script data-card-contents-for-ri="6947" type="text/json">{"id":6947,"name":"Medical Microbiology","url":"https://www.academia.edu/Documents/in/Medical_Microbiology?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="7710" rel="nofollow" href="https://www.academia.edu/Documents/in/Biology">Biology</a><script data-card-contents-for-ri="7710" type="text/json">{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology?f_ri=1444","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=76142990]'), work: {"id":76142990,"title":"Susceptibility profile of clinical and environmental isolates ofCryptococcus neoformansandCryptococcus gattiiin Uberaba, Minas Gerais, Brazil","created_at":"2022-04-11T10:59:34.124-07:00","url":"https://www.academia.edu/76142990/Susceptibility_profile_of_clinical_and_environmental_isolates_ofCryptococcus_neoformansandCryptococcus_gattiiin_Uberaba_Minas_Gerais_Brazil?f_ri=1444","dom_id":"work_76142990","summary":"formerly considered to be restricted to tropical and subtropical regions, but several outbreaks of cryptococcosis involving both immunocompetent and immunocompromised individuals have been reported in Canada, the US Pacifi c Northwest, Australia, and South America, indicating its geographic expansion [2,3]. In Brazil, C. neoformans is distributed throughout the country, whereas C. gattii is mainly found in the North and Northeast regions [4]. Due to the increase in the number of immunocompromised individuals over the past several decades, the prevalence of invasive fungal infections has substantially increased worldwide [5]. This has led to high rates of therapeutic and/or prophylactic use of azole derivates and other antifungals, causing selective epidemiological pressure, which may have contributed to the decreased susceptibility to these drugs [6].","downloadable_attachments":[{"id":83809330,"asset_id":76142990,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":35447045,"first_name":"Delio","last_name":"Mora","domain_name":"ufsb","page_name":"DelioMora","display_name":"Delio J Mora","profile_url":"https://ufsb.academia.edu/DelioMora?f_ri=1444","photo":"https://0.academia-photos.com/35447045/21349938/20761024/s65_delio.mora.jpg"}],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=1444","nofollow":true},{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true},{"id":6947,"name":"Medical Microbiology","url":"https://www.academia.edu/Documents/in/Medical_Microbiology?f_ri=1444","nofollow":true},{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology?f_ri=1444","nofollow":true},{"id":9821,"name":"Brazil","url":"https://www.academia.edu/Documents/in/Brazil?f_ri=1444"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine?f_ri=1444"},{"id":28594,"name":"Medical Mycology","url":"https://www.academia.edu/Documents/in/Medical_Mycology?f_ri=1444"},{"id":28596,"name":"Cryptococcus gattii","url":"https://www.academia.edu/Documents/in/Cryptococcus_gattii?f_ri=1444"},{"id":95046,"name":"Cryptococcus Neoformans","url":"https://www.academia.edu/Documents/in/Cryptococcus_Neoformans?f_ri=1444"},{"id":552209,"name":"Antifungal Agents","url":"https://www.academia.edu/Documents/in/Antifungal_Agents?f_ri=1444"},{"id":561014,"name":"Microbial Sensitivity Tests","url":"https://www.academia.edu/Documents/in/Microbial_Sensitivity_Tests?f_ri=1444"},{"id":1395667,"name":"Cryptococcosis","url":"https://www.academia.edu/Documents/in/Cryptococcosis?f_ri=1444"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_64876166" data-work_id="64876166" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/64876166/Routes_of_phlogopite_weathering_by_three_fungal_strains">Routes of phlogopite weathering by three fungal strains</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Fungi dissolve soil minerals by acidifying their microenvironments, exuding chelating molecules, and by mechanical disruption of the crystal lattice. Dissolution may occur at two scales: microscale (surface of contact between fungus and... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_64876166" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Fungi dissolve soil minerals by acidifying their microenvironments, exuding chelating molecules, and by mechanical disruption of the crystal lattice. Dissolution may occur at two scales: microscale (surface of contact between fungus and mineral) and medium scale (affecting entire mineral grains). Mineral weathering by fungi and other microorganisms is being intensely investigated as thought to be a significant global contribution to weathering, perhaps also modifying weathering products, especially clay minerals. Here we report fungal dissolution of phlogopite (Mg-Fe-rich mica) in experiments with three fungal strains (Alternaria tenuissima, Cladosporium cladosporioides and Stilbella sp.) grown on solid medium for 30 days at 21 °C and 96-100% relative humidity. The focus was to investigate the chemical changes induced by the fungi on phlogopite, translocation of micronutrients to the mycelium and the possible differences between the three species. The study used variablepressure SEM-EDS equipped with both secondary electrons with charge contrast imaging and backscattered electrons. Statistical analysis of the results (principal component and discriminant analysis) discriminated between the weathering activities of the three fungal species, which increased from Stilbella to C. cladosporioides to A. tenuissima, in agreement with the respective decreasing pH values measured in the media (6.4, 5.8, 5.2 ± 0.03). Phlogopite weathering features were irregular and variable (contrast change, troughs, lateral dissolution, flake thinning, breakdown), apparently not caused by direct contact with fungal hyphae. EDS values indicated several weathering stages and two or more dissolution mechanisms, one of them suggesting cation rearrangement in the mica towards decreasing octahedral and interlayer cation contents that produced Al-rich smectite. Intimate fungusmineral interaction was observed as hyphal attachment to phlogopite surfaces, penetration between sheets at the edges (where phlogopite structure is more labile) and changes in the 3 contrast of the mica surface around attached hyphae. The lack of observable dissolution traces from such contact interaction is interpreted as the result of effacing by the more intense acid leaching operating at larger scale.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/64876166" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="7e4edb2b5a7e3f905972c9ffa6f8d937" rel="nofollow" data-download="{"attachment_id":76706674,"asset_id":64876166,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/76706674/download_file?st=MTc0MDU3NTMzMyw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="38753647" href="https://entecra.academia.edu/RosarioNapoli">Rosario Napoli</a><script data-card-contents-for-user="38753647" type="text/json">{"id":38753647,"first_name":"Rosario","last_name":"Napoli","domain_name":"entecra","page_name":"RosarioNapoli","display_name":"Rosario Napoli","profile_url":"https://entecra.academia.edu/RosarioNapoli?f_ri=1444","photo":"https://0.academia-photos.com/38753647/31807218/29033069/s65_rosario.napoli.jpg"}</script></span></span></li><li class="js-paper-rank-work_64876166 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="64876166"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 64876166, container: ".js-paper-rank-work_64876166", }); 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$(".js-view-count[data-work-id=64876166]").text(description); $(".js-view-count-work_64876166").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_64876166").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="64876166"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">11</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="159" rel="nofollow" href="https://www.academia.edu/Documents/in/Microbiology">Microbiology</a>, <script data-card-contents-for-ri="159" type="text/json">{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="1444" rel="nofollow" href="https://www.academia.edu/Documents/in/Environmental_microbiology">Environmental microbiology</a>, <script data-card-contents-for-ri="1444" type="text/json">{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="5541" rel="nofollow" href="https://www.academia.edu/Documents/in/Plant_Biology">Plant Biology</a>, <script data-card-contents-for-ri="5541" type="text/json">{"id":5541,"name":"Plant Biology","url":"https://www.academia.edu/Documents/in/Plant_Biology?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="7710" rel="nofollow" href="https://www.academia.edu/Documents/in/Biology">Biology</a><script data-card-contents-for-ri="7710" type="text/json">{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology?f_ri=1444","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=64876166]'), work: {"id":64876166,"title":"Routes of phlogopite weathering by three fungal strains","created_at":"2021-12-17T09:29:41.051-08:00","url":"https://www.academia.edu/64876166/Routes_of_phlogopite_weathering_by_three_fungal_strains?f_ri=1444","dom_id":"work_64876166","summary":"Fungi dissolve soil minerals by acidifying their microenvironments, exuding chelating molecules, and by mechanical disruption of the crystal lattice. Dissolution may occur at two scales: microscale (surface of contact between fungus and mineral) and medium scale (affecting entire mineral grains). Mineral weathering by fungi and other microorganisms is being intensely investigated as thought to be a significant global contribution to weathering, perhaps also modifying weathering products, especially clay minerals. Here we report fungal dissolution of phlogopite (Mg-Fe-rich mica) in experiments with three fungal strains (Alternaria tenuissima, Cladosporium cladosporioides and Stilbella sp.) grown on solid medium for 30 days at 21 °C and 96-100% relative humidity. The focus was to investigate the chemical changes induced by the fungi on phlogopite, translocation of micronutrients to the mycelium and the possible differences between the three species. The study used variablepressure SEM-EDS equipped with both secondary electrons with charge contrast imaging and backscattered electrons. Statistical analysis of the results (principal component and discriminant analysis) discriminated between the weathering activities of the three fungal species, which increased from Stilbella to C. cladosporioides to A. tenuissima, in agreement with the respective decreasing pH values measured in the media (6.4, 5.8, 5.2 ± 0.03). Phlogopite weathering features were irregular and variable (contrast change, troughs, lateral dissolution, flake thinning, breakdown), apparently not caused by direct contact with fungal hyphae. EDS values indicated several weathering stages and two or more dissolution mechanisms, one of them suggesting cation rearrangement in the mica towards decreasing octahedral and interlayer cation contents that produced Al-rich smectite. Intimate fungusmineral interaction was observed as hyphal attachment to phlogopite surfaces, penetration between sheets at the edges (where phlogopite structure is more labile) and changes in the 3 contrast of the mica surface around attached hyphae. The lack of observable dissolution traces from such contact interaction is interpreted as the result of effacing by the more intense acid leaching operating at larger scale.","downloadable_attachments":[{"id":76706674,"asset_id":64876166,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":38753647,"first_name":"Rosario","last_name":"Napoli","domain_name":"entecra","page_name":"RosarioNapoli","display_name":"Rosario Napoli","profile_url":"https://entecra.academia.edu/RosarioNapoli?f_ri=1444","photo":"https://0.academia-photos.com/38753647/31807218/29033069/s65_rosario.napoli.jpg"}],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=1444","nofollow":true},{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true},{"id":5541,"name":"Plant Biology","url":"https://www.academia.edu/Documents/in/Plant_Biology?f_ri=1444","nofollow":true},{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology?f_ri=1444","nofollow":true},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine?f_ri=1444"},{"id":133177,"name":"Temperature","url":"https://www.academia.edu/Documents/in/Temperature?f_ri=1444"},{"id":357551,"name":"Fungal Biology","url":"https://www.academia.edu/Documents/in/Fungal_Biology?f_ri=1444"},{"id":957359,"name":"Culture Media","url":"https://www.academia.edu/Documents/in/Culture_Media?f_ri=1444"},{"id":1137254,"name":"Hydrogen-Ion Concentration","url":"https://www.academia.edu/Documents/in/Hydrogen-Ion_Concentration?f_ri=1444"},{"id":1753806,"name":"Alternaria","url":"https://www.academia.edu/Documents/in/Alternaria?f_ri=1444"},{"id":1943522,"name":"Cladosporium","url":"https://www.academia.edu/Documents/in/Cladosporium?f_ri=1444"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_62656400" data-work_id="62656400" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/62656400/Biofilm_formation_by_Staphylococcus_aureus_from_food_contact_surfaces_in_a_meat_based_broth_and_sensitivity_to_sanitizers">Biofilm formation by Staphylococcus aureus from food contact surfaces in a meat-based broth and sensitivity to sanitizers</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">This study assessed the capacity of adhesion, the detachment kinetic and the biofilm formation by Staphylococcus aureus isolated from food services on stainless steel and polypropylene surfaces (2 x 2 cm) when cultivated in a meat-based... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_62656400" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">This study assessed the capacity of adhesion, the detachment kinetic and the biofilm formation by Staphylococcus aureus isolated from food services on stainless steel and polypropylene surfaces (2 x 2 cm) when cultivated in a meat-based broth at 28 and 7°C. It was also to study the efficacy of the sanitizers sodium hypochlorite (250 mg/L) and peracetic acid (30 mg/L) in inactivating the bacterial cells in the preformed biofilm. S. aureus strains adhered in high numbers regardless the assayed surface kind and incubation temperature over 72 h. Cells detachment of surfaces revealed high persistence over the incubation period. Number of cells needed for biofilm formation was noted at all experimental systems already after 3 days. Peracetic acid and sodium hypochlorite were not efficient in completely removing the cells of S. aureus adhered on polypropylene and stainless steel surfaces. From these results, the assayed strains revealed high capacity to adhere and form biofilm on polypropylene and stainless steel surfaces under different growth conditions. Moreover, the cells in biofilm matrix were resistant for total removal when submitted to the exposure to sanitizers.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/62656400" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="f6173e68f09f1bbede3f0afe90ae139f" rel="nofollow" data-download="{"attachment_id":75346093,"asset_id":62656400,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/75346093/download_file?st=MTc0MDU3NTMzMyw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="107890458" href="https://ufcg.academia.edu/AnaAthayde">Ana Athayde</a><script data-card-contents-for-user="107890458" type="text/json">{"id":107890458,"first_name":"Ana","last_name":"Athayde","domain_name":"ufcg","page_name":"AnaAthayde","display_name":"Ana Athayde","profile_url":"https://ufcg.academia.edu/AnaAthayde?f_ri=1444","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_62656400 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="62656400"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 62656400, container: ".js-paper-rank-work_62656400", }); 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$(".js-view-count[data-work-id=62656400]").text(description); $(".js-view-count-work_62656400").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_62656400").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="62656400"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">13</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="159" rel="nofollow" href="https://www.academia.edu/Documents/in/Microbiology">Microbiology</a>, <script data-card-contents-for-ri="159" type="text/json">{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="1444" rel="nofollow" href="https://www.academia.edu/Documents/in/Environmental_microbiology">Environmental microbiology</a>, <script data-card-contents-for-ri="1444" type="text/json">{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="2187" rel="nofollow" href="https://www.academia.edu/Documents/in/Biofilms">Biofilms</a>, <script data-card-contents-for-ri="2187" type="text/json">{"id":2187,"name":"Biofilms","url":"https://www.academia.edu/Documents/in/Biofilms?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="6947" rel="nofollow" href="https://www.academia.edu/Documents/in/Medical_Microbiology">Medical Microbiology</a><script data-card-contents-for-ri="6947" type="text/json">{"id":6947,"name":"Medical Microbiology","url":"https://www.academia.edu/Documents/in/Medical_Microbiology?f_ri=1444","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=62656400]'), work: {"id":62656400,"title":"Biofilm formation by Staphylococcus aureus from food contact surfaces in a meat-based broth and sensitivity to sanitizers","created_at":"2021-11-28T15:57:55.501-08:00","url":"https://www.academia.edu/62656400/Biofilm_formation_by_Staphylococcus_aureus_from_food_contact_surfaces_in_a_meat_based_broth_and_sensitivity_to_sanitizers?f_ri=1444","dom_id":"work_62656400","summary":"This study assessed the capacity of adhesion, the detachment kinetic and the biofilm formation by Staphylococcus aureus isolated from food services on stainless steel and polypropylene surfaces (2 x 2 cm) when cultivated in a meat-based broth at 28 and 7°C. It was also to study the efficacy of the sanitizers sodium hypochlorite (250 mg/L) and peracetic acid (30 mg/L) in inactivating the bacterial cells in the preformed biofilm. S. aureus strains adhered in high numbers regardless the assayed surface kind and incubation temperature over 72 h. Cells detachment of surfaces revealed high persistence over the incubation period. Number of cells needed for biofilm formation was noted at all experimental systems already after 3 days. Peracetic acid and sodium hypochlorite were not efficient in completely removing the cells of S. aureus adhered on polypropylene and stainless steel surfaces. From these results, the assayed strains revealed high capacity to adhere and form biofilm on polypropylene and stainless steel surfaces under different growth conditions. Moreover, the cells in biofilm matrix were resistant for total removal when submitted to the exposure to sanitizers.","downloadable_attachments":[{"id":75346093,"asset_id":62656400,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":107890458,"first_name":"Ana","last_name":"Athayde","domain_name":"ufcg","page_name":"AnaAthayde","display_name":"Ana Athayde","profile_url":"https://ufcg.academia.edu/AnaAthayde?f_ri=1444","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=1444","nofollow":true},{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true},{"id":2187,"name":"Biofilms","url":"https://www.academia.edu/Documents/in/Biofilms?f_ri=1444","nofollow":true},{"id":6947,"name":"Medical Microbiology","url":"https://www.academia.edu/Documents/in/Medical_Microbiology?f_ri=1444","nofollow":true},{"id":116078,"name":"Staphylococcus aureus","url":"https://www.academia.edu/Documents/in/Staphylococcus_aureus?f_ri=1444"},{"id":133177,"name":"Temperature","url":"https://www.academia.edu/Documents/in/Temperature?f_ri=1444"},{"id":139817,"name":"Disinfectants","url":"https://www.academia.edu/Documents/in/Disinfectants?f_ri=1444"},{"id":342520,"name":"Sodium Hypochlorite","url":"https://www.academia.edu/Documents/in/Sodium_Hypochlorite?f_ri=1444"},{"id":413195,"name":"Time Factors","url":"https://www.academia.edu/Documents/in/Time_Factors?f_ri=1444"},{"id":561014,"name":"Microbial Sensitivity Tests","url":"https://www.academia.edu/Documents/in/Microbial_Sensitivity_Tests?f_ri=1444"},{"id":1109980,"name":"Bacterial Adhesion","url":"https://www.academia.edu/Documents/in/Bacterial_Adhesion?f_ri=1444"},{"id":1478014,"name":"Peracetic Acid","url":"https://www.academia.edu/Documents/in/Peracetic_Acid?f_ri=1444"},{"id":2700441,"name":"food handling","url":"https://www.academia.edu/Documents/in/food_handling?f_ri=1444"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_59722128" data-work_id="59722128" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/59722128/Working_relationships_of_infection_prevention_and_control_programs_and_environmental_services_and_associations_with_antibiotic_resistant_organisms_in_Canadian_acute_care_hospitals">Working relationships of infection prevention and control programs and environmental services and associations with antibiotic-resistant organisms in Canadian acute care hospitals</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Background: Environmental contamination in hospitals with antibiotic-resistant organisms (AROs) is associated with patient contraction of AROs. This study examined the working relationship of Infection Prevention and Control (IPAC) and... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_59722128" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Background: Environmental contamination in hospitals with antibiotic-resistant organisms (AROs) is associated with patient contraction of AROs. This study examined the working relationship of Infection Prevention and Control (IPAC) and Environmental Services and the impact of that relationship on ARO rates. Methods: Lead infection control professionals completed an online survey that assessed the IPAC and Environmental Services working relationship in their acute care hospital in 2011. The survey assessed cleaning collaborations, staff training, hospital cleanliness, and nosocomial methicillin-resistant Staphylococcus aureus (MRSA) infection, vancomycin-resistant Enterococcus (VRE) infection, and Clostridium difficile infection (CDI). Results: The survey was completed by 58.3% of hospitals (119 of 204). Two-thirds (65.8%; 77 of 117) of the respondents reported that their cleaners were adequately trained, and 62.4% (73 of 117) reported that their hospital was sufficiently clean. Greater cooperation between IPAC and Environmental Services was associated with lower rates of MRSA infection (r ¼ À0.22; P ¼ .02), and frequent collaboration regarding cleaning protocols was associated with lower rates of VRE infection (r ¼ À0.20; P ¼ .03) and CDI (r ¼ À0.31; P < .001). Conclusions: Canadian IPAC programs generally had collaborative working relationships with Environmental Services, and this was associated with lower rates of ARO. Deficits in the adequacy of cleaning staff training and hospital cleanliness were identified. The promotion of collaborative working relationships and additional training for Environmental Services workers would be expected to lower ARO rates.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/59722128" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="9db3a3d56333ec518cd2597b6fa043e4" rel="nofollow" data-download="{"attachment_id":73498639,"asset_id":59722128,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/73498639/download_file?st=MTc0MDU3NTMzMyw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="90706354" href="https://independent.academia.edu/DickZoutman">Dick Zoutman</a><script data-card-contents-for-user="90706354" type="text/json">{"id":90706354,"first_name":"Dick","last_name":"Zoutman","domain_name":"independent","page_name":"DickZoutman","display_name":"Dick Zoutman","profile_url":"https://independent.academia.edu/DickZoutman?f_ri=1444","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_59722128 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="59722128"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 59722128, container: ".js-paper-rank-work_59722128", }); 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$(".js-view-count[data-work-id=59722128]").text(description); $(".js-view-count-work_59722128").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_59722128").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="59722128"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">11</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="588" rel="nofollow" href="https://www.academia.edu/Documents/in/Nursing">Nursing</a>, <script data-card-contents-for-ri="588" type="text/json">{"id":588,"name":"Nursing","url":"https://www.academia.edu/Documents/in/Nursing?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="1444" rel="nofollow" href="https://www.academia.edu/Documents/in/Environmental_microbiology">Environmental microbiology</a>, <script data-card-contents-for-ri="1444" type="text/json">{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="16635" rel="nofollow" href="https://www.academia.edu/Documents/in/Health_Services_Research">Health Services Research</a>, <script data-card-contents-for-ri="16635" type="text/json">{"id":16635,"name":"Health Services Research","url":"https://www.academia.edu/Documents/in/Health_Services_Research?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="54114" rel="nofollow" href="https://www.academia.edu/Documents/in/Canada">Canada</a><script data-card-contents-for-ri="54114" type="text/json">{"id":54114,"name":"Canada","url":"https://www.academia.edu/Documents/in/Canada?f_ri=1444","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=59722128]'), work: {"id":59722128,"title":"Working relationships of infection prevention and control programs and environmental services and associations with antibiotic-resistant organisms in Canadian acute care hospitals","created_at":"2021-10-23T18:57:43.863-07:00","url":"https://www.academia.edu/59722128/Working_relationships_of_infection_prevention_and_control_programs_and_environmental_services_and_associations_with_antibiotic_resistant_organisms_in_Canadian_acute_care_hospitals?f_ri=1444","dom_id":"work_59722128","summary":"Background: Environmental contamination in hospitals with antibiotic-resistant organisms (AROs) is associated with patient contraction of AROs. This study examined the working relationship of Infection Prevention and Control (IPAC) and Environmental Services and the impact of that relationship on ARO rates. Methods: Lead infection control professionals completed an online survey that assessed the IPAC and Environmental Services working relationship in their acute care hospital in 2011. The survey assessed cleaning collaborations, staff training, hospital cleanliness, and nosocomial methicillin-resistant Staphylococcus aureus (MRSA) infection, vancomycin-resistant Enterococcus (VRE) infection, and Clostridium difficile infection (CDI). Results: The survey was completed by 58.3% of hospitals (119 of 204). Two-thirds (65.8%; 77 of 117) of the respondents reported that their cleaners were adequately trained, and 62.4% (73 of 117) reported that their hospital was sufficiently clean. Greater cooperation between IPAC and Environmental Services was associated with lower rates of MRSA infection (r ¼ À0.22; P ¼ .02), and frequent collaboration regarding cleaning protocols was associated with lower rates of VRE infection (r ¼ À0.20; P ¼ .03) and CDI (r ¼ À0.31; P \u003c .001). Conclusions: Canadian IPAC programs generally had collaborative working relationships with Environmental Services, and this was associated with lower rates of ARO. Deficits in the adequacy of cleaning staff training and hospital cleanliness were identified. The promotion of collaborative working relationships and additional training for Environmental Services workers would be expected to lower ARO rates.","downloadable_attachments":[{"id":73498639,"asset_id":59722128,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":90706354,"first_name":"Dick","last_name":"Zoutman","domain_name":"independent","page_name":"DickZoutman","display_name":"Dick Zoutman","profile_url":"https://independent.academia.edu/DickZoutman?f_ri=1444","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":588,"name":"Nursing","url":"https://www.academia.edu/Documents/in/Nursing?f_ri=1444","nofollow":true},{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true},{"id":16635,"name":"Health Services Research","url":"https://www.academia.edu/Documents/in/Health_Services_Research?f_ri=1444","nofollow":true},{"id":54114,"name":"Canada","url":"https://www.academia.edu/Documents/in/Canada?f_ri=1444","nofollow":true},{"id":55155,"name":"Emergency Medical Services","url":"https://www.academia.edu/Documents/in/Emergency_Medical_Services?f_ri=1444"},{"id":69637,"name":"Infection Control","url":"https://www.academia.edu/Documents/in/Infection_Control?f_ri=1444"},{"id":153168,"name":"Data Collection","url":"https://www.academia.edu/Documents/in/Data_Collection?f_ri=1444"},{"id":410370,"name":"Public health systems and services research","url":"https://www.academia.edu/Documents/in/Public_health_systems_and_services_research-1?f_ri=1444"},{"id":587528,"name":"Bacterial infections","url":"https://www.academia.edu/Documents/in/Bacterial_infections?f_ri=1444"},{"id":1153482,"name":"Cooperative Behavior","url":"https://www.academia.edu/Documents/in/Cooperative_Behavior?f_ri=1444"},{"id":3854113,"name":"Cross Infection","url":"https://www.academia.edu/Documents/in/Cross_Infection?f_ri=1444"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_1583648" data-work_id="1583648" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/1583648/Microbial_ecology_of_the_closed_artificial_ecosystem_MELiSSA_Micro_Ecological_Life_Support_System_Alternative_Reinventing_and_compartmentalizing_the_Earth_s_food_and_oxygen_regeneration_system_for_long_haul_space_exploration_missions">Microbial ecology of the closed artificial ecosystem MELiSSA (Micro-Ecological Life Support System Alternative): Reinventing and compartmentalizing the Earth’s food and oxygen regeneration system for long-haul space exploration missions</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">MELiSSA is a bioregenerative life support system designed by the European Space Agency (ESA) for the complete recycling of gas, liquid and solid wastes during long distance space exploration. The system uses the combined activity of... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_1583648" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">MELiSSA is a bioregenerative life support system designed by the European Space Agency (ESA) for the complete recycling of gas, liquid and solid wastes during long distance space exploration. The system uses the combined activity of different living organisms: microbial cultures in bioreactors, a plant compartment and a human crew. In this minireview, the development of a short-cut ecological system for the biotransformation of organic waste is discussed from a microorganism’s perspective. The artificial ecological model—still in full development—that is inspired by Earth’s own geomicrobiological ecosystem serves as an ideal study object on microbial ecology and will become an indispensable travel<br />companion in manned space exploration.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/1583648" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="ae0456a40fe195990e24093d17443267" rel="nofollow" data-download="{"attachment_id":14616786,"asset_id":1583648,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/14616786/download_file?st=MTc0MDU3NTMzMyw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="1802395" href="https://sckcen.academia.edu/PaulJanssen">Paul Janssen</a><script data-card-contents-for-user="1802395" type="text/json">{"id":1802395,"first_name":"Paul","last_name":"Janssen","domain_name":"sckcen","page_name":"PaulJanssen","display_name":"Paul Janssen","profile_url":"https://sckcen.academia.edu/PaulJanssen?f_ri=1444","photo":"https://0.academia-photos.com/1802395/615831/31334009/s65_paul.janssen.jpg"}</script></span></span></li><li class="js-paper-rank-work_1583648 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="1583648"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 1583648, container: ".js-paper-rank-work_1583648", }); 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$(".js-view-count[data-work-id=1583648]").text(description); $(".js-view-count-work_1583648").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_1583648").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="1583648"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">17</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="159" rel="nofollow" href="https://www.academia.edu/Documents/in/Microbiology">Microbiology</a>, <script data-card-contents-for-ri="159" type="text/json">{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="1444" rel="nofollow" href="https://www.academia.edu/Documents/in/Environmental_microbiology">Environmental microbiology</a>, <script data-card-contents-for-ri="1444" type="text/json">{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="6389" rel="nofollow" href="https://www.academia.edu/Documents/in/Microbial_Ecology">Microbial Ecology</a>, <script data-card-contents-for-ri="6389" type="text/json">{"id":6389,"name":"Microbial Ecology","url":"https://www.academia.edu/Documents/in/Microbial_Ecology?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="6947" rel="nofollow" href="https://www.academia.edu/Documents/in/Medical_Microbiology">Medical Microbiology</a><script data-card-contents-for-ri="6947" type="text/json">{"id":6947,"name":"Medical Microbiology","url":"https://www.academia.edu/Documents/in/Medical_Microbiology?f_ri=1444","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=1583648]'), work: {"id":1583648,"title":"Microbial ecology of the closed artificial ecosystem MELiSSA (Micro-Ecological Life Support System Alternative): Reinventing and compartmentalizing the Earth’s food and oxygen regeneration system for long-haul space exploration missions","created_at":"2012-05-22T20:54:10.401-07:00","url":"https://www.academia.edu/1583648/Microbial_ecology_of_the_closed_artificial_ecosystem_MELiSSA_Micro_Ecological_Life_Support_System_Alternative_Reinventing_and_compartmentalizing_the_Earth_s_food_and_oxygen_regeneration_system_for_long_haul_space_exploration_missions?f_ri=1444","dom_id":"work_1583648","summary":"MELiSSA is a bioregenerative life support system designed by the European Space Agency (ESA) for the complete recycling of gas, liquid and solid wastes during long distance space exploration. The system uses the combined activity of different living organisms: microbial cultures in bioreactors, a plant compartment and a human crew. In this minireview, the development of a short-cut ecological system for the biotransformation of organic waste is discussed from a microorganism’s perspective. The artificial ecological model—still in full development—that is inspired by Earth’s own geomicrobiological ecosystem serves as an ideal study object on microbial ecology and will become an indispensable travel\ncompanion in manned space exploration.","downloadable_attachments":[{"id":14616786,"asset_id":1583648,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":1802395,"first_name":"Paul","last_name":"Janssen","domain_name":"sckcen","page_name":"PaulJanssen","display_name":"Paul Janssen","profile_url":"https://sckcen.academia.edu/PaulJanssen?f_ri=1444","photo":"https://0.academia-photos.com/1802395/615831/31334009/s65_paul.janssen.jpg"}],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=1444","nofollow":true},{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true},{"id":6389,"name":"Microbial Ecology","url":"https://www.academia.edu/Documents/in/Microbial_Ecology?f_ri=1444","nofollow":true},{"id":6947,"name":"Medical Microbiology","url":"https://www.academia.edu/Documents/in/Medical_Microbiology?f_ri=1444","nofollow":true},{"id":7603,"name":"Food","url":"https://www.academia.edu/Documents/in/Food?f_ri=1444"},{"id":37884,"name":"Space Exploration","url":"https://www.academia.edu/Documents/in/Space_Exploration?f_ri=1444"},{"id":46379,"name":"Life Support Systems","url":"https://www.academia.edu/Documents/in/Life_Support_Systems?f_ri=1444"},{"id":66244,"name":"Space flight","url":"https://www.academia.edu/Documents/in/Space_flight?f_ri=1444"},{"id":110903,"name":"Solid waste","url":"https://www.academia.edu/Documents/in/Solid_waste?f_ri=1444"},{"id":113903,"name":"Bacteria","url":"https://www.academia.edu/Documents/in/Bacteria?f_ri=1444"},{"id":133873,"name":"Plants","url":"https://www.academia.edu/Documents/in/Plants?f_ri=1444"},{"id":297270,"name":"Ecological Model","url":"https://www.academia.edu/Documents/in/Ecological_Model?f_ri=1444"},{"id":380825,"name":"Oxygen","url":"https://www.academia.edu/Documents/in/Oxygen?f_ri=1444"},{"id":501201,"name":"Bioreactors","url":"https://www.academia.edu/Documents/in/Bioreactors?f_ri=1444"},{"id":751569,"name":"Organic Waste","url":"https://www.academia.edu/Documents/in/Organic_Waste?f_ri=1444"},{"id":1150983,"name":"Support System","url":"https://www.academia.edu/Documents/in/Support_System?f_ri=1444"},{"id":1714028,"name":"Long Distance","url":"https://www.academia.edu/Documents/in/Long_Distance?f_ri=1444"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_6917634" data-work_id="6917634" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" 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microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true},{"id":7125,"name":"Culture","url":"https://www.academia.edu/Documents/in/Culture?f_ri=1444","nofollow":true},{"id":7742,"name":"Glass","url":"https://www.academia.edu/Documents/in/Glass?f_ri=1444","nofollow":true},{"id":28235,"name":"Multidisciplinary","url":"https://www.academia.edu/Documents/in/Multidisciplinary?f_ri=1444","nofollow":true},{"id":37434,"name":"Quantitative analysis","url":"https://www.academia.edu/Documents/in/Quantitative_analysis?f_ri=1444"},{"id":37894,"name":"Quantitative PCR","url":"https://www.academia.edu/Documents/in/Quantitative_PCR?f_ri=1444"},{"id":59249,"name":"Computers","url":"https://www.academia.edu/Documents/in/Computers?f_ri=1444"},{"id":71578,"name":"Wood","url":"https://www.academia.edu/Documents/in/Wood?f_ri=1444"},{"id":73645,"name":"Sampling methods","url":"https://www.academia.edu/Documents/in/Sampling_methods?f_ri=1444"},{"id":96502,"name":"Applied","url":"https://www.academia.edu/Documents/in/Applied?f_ri=1444"},{"id":107083,"name":"Method","url":"https://www.academia.edu/Documents/in/Method?f_ri=1444"},{"id":107664,"name":"Applied Environmental Microbiology","url":"https://www.academia.edu/Documents/in/Applied_Environmental_Microbiology?f_ri=1444"},{"id":113903,"name":"Bacteria","url":"https://www.academia.edu/Documents/in/Bacteria?f_ri=1444"},{"id":118339,"name":"Polymerase Chain Reaction","url":"https://www.academia.edu/Documents/in/Polymerase_Chain_Reaction?f_ri=1444"},{"id":335965,"name":"Gram-negative bacteria","url":"https://www.academia.edu/Documents/in/Gram-negative_bacteria?f_ri=1444"},{"id":620243,"name":"Erwinia","url":"https://www.academia.edu/Documents/in/Erwinia?f_ri=1444"},{"id":641216,"name":"Quantitative Analysis","url":"https://www.academia.edu/Documents/in/Quantitative_Analysis-1?f_ri=1444"},{"id":872313,"name":"Collection Efficiency","url":"https://www.academia.edu/Documents/in/Collection_Efficiency?f_ri=1444"},{"id":901876,"name":"Sensitivity and Specificity","url":"https://www.academia.edu/Documents/in/Sensitivity_and_Specificity?f_ri=1444"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_28128580" data-work_id="28128580" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/28128580/Biofilm_formation_and_cellulose_expression_among_diverse_environmental_Pseudomonas_isolates">Biofilm formation and cellulose expression among diverse environmental Pseudomonas isolates</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">The ability to form biofilms is seen as an increasingly important colonization strategy among both pathogenic and environmental bacteria. A survey of 185 plant-associated, phytopathogenic, soil and river Pseudomonas isolates resulted in... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_28128580" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">The ability to form biofilms is seen as an increasingly important colonization strategy among both pathogenic and environmental bacteria. A survey of 185 plant-associated, phytopathogenic, soil and river Pseudomonas isolates resulted in 76% producing biofilms at the air-liquid (A-L) interface after selection in static microcosms. Considerable variation in biofilm phenotype was observed, including waxy aggregations, viscous and floccular masses, and physically cohesive biofilms with continuously varying strengths over 1500-fold. Calcofluor epifluorescent microscopy identified cellulose as the matrix component in biofilms produced by Pseudomonas asplenii, Pseudomonas corrugata , Pseudomonas fluorescens , Pseudomonas marginalis , Pseudomonas putida, Pseudomonas savastanoi and Pseudomonas syringae isolates. Cellulose expression and biofilm formation could be induced by the constitutively active WspR19 mutant of the cyclic-di-GMP-associated, GGDEF domain-containing response regulator involved in the P. fluorescens SBW25 wrinkly spreader phenotype and cellular aggregation in Pseudomonas aeruginosa PA01. WspR19 could also induce P. putida KT2440, which otherwise did not produce a biofilm or express cellulose, as well as Escherichia coli K12 and Salmonella typhimurium LT2, both of which express cellulose yet lack WspR homologues. Statistical analysis of biofilm parameters suggest that biofilm development is a more complex process than that simply described by the production of attachment and matrix components and bacterial growth. This complexity was also seen in multivariate analysis as a species-ecological habitat effect, underscoring the fact that in vitro biofilms are abstractions of those surface and volume colonization processes used by bacteria in their natural environments.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/28128580" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="ec73ef4d6fa1bd85e2f7e55117d4f63f" rel="nofollow" data-download="{"attachment_id":48443981,"asset_id":28128580,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/48443981/download_file?st=MTc0MDU3NTMzMyw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="46302747" href="https://abertay.academia.edu/Spiers">Andrew J Spiers</a><script data-card-contents-for-user="46302747" type="text/json">{"id":46302747,"first_name":"Andrew","last_name":"Spiers","domain_name":"abertay","page_name":"Spiers","display_name":"Andrew J Spiers","profile_url":"https://abertay.academia.edu/Spiers?f_ri=1444","photo":"https://0.academia-photos.com/46302747/12253777/13644907/s65_ashton.p.jpg"}</script></span></span></li><li class="js-paper-rank-work_28128580 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="28128580"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 28128580, container: ".js-paper-rank-work_28128580", }); 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A survey of 185 plant-associated, phytopathogenic, soil and river Pseudomonas isolates resulted in 76% producing biofilms at the air-liquid (A-L) interface after selection in static microcosms. Considerable variation in biofilm phenotype was observed, including waxy aggregations, viscous and floccular masses, and physically cohesive biofilms with continuously varying strengths over 1500-fold. Calcofluor epifluorescent microscopy identified cellulose as the matrix component in biofilms produced by Pseudomonas asplenii, Pseudomonas corrugata , Pseudomonas fluorescens , Pseudomonas marginalis , Pseudomonas putida, Pseudomonas savastanoi and Pseudomonas syringae isolates. Cellulose expression and biofilm formation could be induced by the constitutively active WspR19 mutant of the cyclic-di-GMP-associated, GGDEF domain-containing response regulator involved in the P. fluorescens SBW25 wrinkly spreader phenotype and cellular aggregation in Pseudomonas aeruginosa PA01. WspR19 could also induce P. putida KT2440, which otherwise did not produce a biofilm or express cellulose, as well as Escherichia coli K12 and Salmonella typhimurium LT2, both of which express cellulose yet lack WspR homologues. Statistical analysis of biofilm parameters suggest that biofilm development is a more complex process than that simply described by the production of attachment and matrix components and bacterial growth. This complexity was also seen in multivariate analysis as a species-ecological habitat effect, underscoring the fact that in vitro biofilms are abstractions of those surface and volume colonization processes used by bacteria in their natural environments.","downloadable_attachments":[{"id":48443981,"asset_id":28128580,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":46302747,"first_name":"Andrew","last_name":"Spiers","domain_name":"abertay","page_name":"Spiers","display_name":"Andrew J Spiers","profile_url":"https://abertay.academia.edu/Spiers?f_ri=1444","photo":"https://0.academia-photos.com/46302747/12253777/13644907/s65_ashton.p.jpg"}],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=1444","nofollow":true},{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true},{"id":2187,"name":"Biofilms","url":"https://www.academia.edu/Documents/in/Biofilms?f_ri=1444","nofollow":true},{"id":37959,"name":"Cellulose","url":"https://www.academia.edu/Documents/in/Cellulose?f_ri=1444","nofollow":true},{"id":112324,"name":"Pseudomonas","url":"https://www.academia.edu/Documents/in/Pseudomonas?f_ri=1444"},{"id":133873,"name":"Plants","url":"https://www.academia.edu/Documents/in/Plants?f_ri=1444"},{"id":213897,"name":"Phenotype","url":"https://www.academia.edu/Documents/in/Phenotype?f_ri=1444"},{"id":231547,"name":"Soil Microbiology","url":"https://www.academia.edu/Documents/in/Soil_Microbiology?f_ri=1444"},{"id":373754,"name":"Ecosystem","url":"https://www.academia.edu/Documents/in/Ecosystem?f_ri=1444"},{"id":646441,"name":"Water Microbiology","url":"https://www.academia.edu/Documents/in/Water_Microbiology?f_ri=1444"},{"id":1109980,"name":"Bacterial Adhesion","url":"https://www.academia.edu/Documents/in/Bacterial_Adhesion?f_ri=1444"},{"id":1133132,"name":"Environmental","url":"https://www.academia.edu/Documents/in/Environmental?f_ri=1444"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_28041971" data-work_id="28041971" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/28041971/Rapid_Detection_and_Quantification_of_Lanthanide_Ions_Using_High_Speed_Polymerase_Chain_Reactions">Rapid Detection and Quantification of Lanthanide Ions Using High-Speed Polymerase Chain Reactions</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Recently, a novel approach to a highly sensitive and quantitative detection of rare earth element (REE) ions including La3+, Eu3+ and Tb3+, by the polymerase chain reaction (PCR) technique, has been reported. The detection of REE ions is... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_28041971" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Recently, a novel approach to a highly sensitive and quantitative detection of rare earth element (REE) ions including La3+, Eu3+ and Tb3+, by the polymerase chain reaction (PCR) technique, has been reported. The detection of REE ions is based on the catalytic nature of REE ions targeting the deoxyribonucleic acid (DNA), thus monitoring of the ions can be achieved by reading the level of intact DNA by PCR. Despite of its high sensitivity (at ppb to ppt levels), the conventional PCR-based REE detection protocol requires certain length of time (1-2 hours). In the present study, we modified the PCR-based REE detection protocols by employing the high-speed PCR, and performed the automated and rapid detection of La3+ in small-sized aqueous samples within 5min.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/28041971" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="f641549a6879432a1cb98ad461a7db86" rel="nofollow" data-download="{"attachment_id":48351204,"asset_id":28041971,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/48351204/download_file?st=MTc0MDU3NTMzMyw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="42266429" href="https://kitakyu-u.academia.edu/TomonoriKawano">Tomonori Kawano</a><script data-card-contents-for-user="42266429" type="text/json">{"id":42266429,"first_name":"Tomonori","last_name":"Kawano","domain_name":"kitakyu-u","page_name":"TomonoriKawano","display_name":"Tomonori Kawano","profile_url":"https://kitakyu-u.academia.edu/TomonoriKawano?f_ri=1444","photo":"https://0.academia-photos.com/42266429/11411154/15409292/s65_tomonori.kawano.png"}</script></span></span></li><li class="js-paper-rank-work_28041971 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="28041971"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 28041971, container: ".js-paper-rank-work_28041971", }); 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The detection of REE ions is based on the catalytic nature of REE ions targeting the deoxyribonucleic acid (DNA), thus monitoring of the ions can be achieved by reading the level of intact DNA by PCR. Despite of its high sensitivity (at ppb to ppt levels), the conventional PCR-based REE detection protocol requires certain length of time (1-2 hours). In the present study, we modified the PCR-based REE detection protocols by employing the high-speed PCR, and performed the automated and rapid detection of La3+ in small-sized aqueous samples within 5min.","downloadable_attachments":[{"id":48351204,"asset_id":28041971,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":42266429,"first_name":"Tomonori","last_name":"Kawano","domain_name":"kitakyu-u","page_name":"TomonoriKawano","display_name":"Tomonori Kawano","profile_url":"https://kitakyu-u.academia.edu/TomonoriKawano?f_ri=1444","photo":"https://0.academia-photos.com/42266429/11411154/15409292/s65_tomonori.kawano.png"}],"research_interests":[{"id":55,"name":"Environmental Engineering","url":"https://www.academia.edu/Documents/in/Environmental_Engineering?f_ri=1444","nofollow":true},{"id":145,"name":"Biochemistry","url":"https://www.academia.edu/Documents/in/Biochemistry?f_ri=1444","nofollow":true},{"id":158,"name":"Marine 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Chemistry","url":"https://www.academia.edu/Documents/in/Analytical_and_Environmental_Chemistry?f_ri=1444"},{"id":759336,"name":"Pollution De L'eau","url":"https://www.academia.edu/Documents/in/Pollution_De_Leau?f_ri=1444"},{"id":962638,"name":"High Sensitivity","url":"https://www.academia.edu/Documents/in/High_Sensitivity?f_ri=1444"},{"id":999797,"name":"Gene Expression by qRT PCR","url":"https://www.academia.edu/Documents/in/Gene_Expression_by_qRT_PCR?f_ri=1444"},{"id":1266840,"name":"PCR/RFLP","url":"https://www.academia.edu/Documents/in/PCR_RFLP-1?f_ri=1444"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_8060235" data-work_id="8060235" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/8060235/Patterns_of_Fecal_Coliform_Contamination_in_Day_Care_Centers">Patterns of Fecal Coliform Contamination in Day-Care Centers</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">During a six-month period, on four separate occasions, six licensed day-care centers had cultures taken from environmental surfaces as well as the hands of children and teachers. Fecal coliforms were recovered from 64 (9.5%) of the 675... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_8060235" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">During a six-month period, on four separate occasions, six licensed day-care centers had cultures taken from environmental surfaces as well as the hands of children and teachers. Fecal coliforms were recovered from 64 (9.5%) of the 675 surfaces sampled. Recovery rate was not influenced by a center's socioeconomic status, time of year, or presence of children who were not toilet trained. Recovery rates did differ significantly in different areas, with the kitchen showing a relatively high recovery rate (19%), and toys and toilets showing remarkably low rates (2% and 4%). Centers with formal hand-washing procedures had lower recovery rates than those without such practices. We also demonstrated a high recovery rate from hands of staff (16%); 6% of children had positive cultures. Contamination of hands and classroom objects is a potential source for the transmission of enteric diseases for children in day-care centers. A program directed at reducing contamination would be important in preventing the spread of diarrheal illness.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/8060235" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="253443017b22dee6231f601fb810fbc6" rel="nofollow" data-download="{"attachment_id":48227700,"asset_id":8060235,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/48227700/download_file?st=MTc0MDU3NTMzMyw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="15541176" href="https://clemson.academia.edu/BHoladay">Bonnie Holaday</a><script data-card-contents-for-user="15541176" type="text/json">{"id":15541176,"first_name":"Bonnie","last_name":"Holaday","domain_name":"clemson","page_name":"BHoladay","display_name":"Bonnie Holaday","profile_url":"https://clemson.academia.edu/BHoladay?f_ri=1444","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_8060235 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="8060235"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 8060235, container: ".js-paper-rank-work_8060235", }); 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$(".js-view-count[data-work-id=8060235]").text(description); $(".js-view-count-work_8060235").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_8060235").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="8060235"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">12</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="588" rel="nofollow" href="https://www.academia.edu/Documents/in/Nursing">Nursing</a>, <script data-card-contents-for-ri="588" type="text/json">{"id":588,"name":"Nursing","url":"https://www.academia.edu/Documents/in/Nursing?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="1444" rel="nofollow" href="https://www.academia.edu/Documents/in/Environmental_microbiology">Environmental microbiology</a>, <script data-card-contents-for-ri="1444" type="text/json">{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="11801" rel="nofollow" href="https://www.academia.edu/Documents/in/Environmental_Monitoring">Environmental Monitoring</a>, <script data-card-contents-for-ri="11801" type="text/json">{"id":11801,"name":"Environmental Monitoring","url":"https://www.academia.edu/Documents/in/Environmental_Monitoring?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="16288" rel="nofollow" href="https://www.academia.edu/Documents/in/Public_Health">Public Health</a><script data-card-contents-for-ri="16288" type="text/json">{"id":16288,"name":"Public Health","url":"https://www.academia.edu/Documents/in/Public_Health?f_ri=1444","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=8060235]'), work: {"id":8060235,"title":"Patterns of Fecal Coliform Contamination in Day-Care Centers","created_at":"2014-08-23T07:16:45.913-07:00","url":"https://www.academia.edu/8060235/Patterns_of_Fecal_Coliform_Contamination_in_Day_Care_Centers?f_ri=1444","dom_id":"work_8060235","summary":"During a six-month period, on four separate occasions, six licensed day-care centers had cultures taken from environmental surfaces as well as the hands of children and teachers. Fecal coliforms were recovered from 64 (9.5%) of the 675 surfaces sampled. Recovery rate was not influenced by a center's socioeconomic status, time of year, or presence of children who were not toilet trained. Recovery rates did differ significantly in different areas, with the kitchen showing a relatively high recovery rate (19%), and toys and toilets showing remarkably low rates (2% and 4%). Centers with formal hand-washing procedures had lower recovery rates than those without such practices. We also demonstrated a high recovery rate from hands of staff (16%); 6% of children had positive cultures. Contamination of hands and classroom objects is a potential source for the transmission of enteric diseases for children in day-care centers. A program directed at reducing contamination would be important in preventing the spread of diarrheal illness.","downloadable_attachments":[{"id":48227700,"asset_id":8060235,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":15541176,"first_name":"Bonnie","last_name":"Holaday","domain_name":"clemson","page_name":"BHoladay","display_name":"Bonnie Holaday","profile_url":"https://clemson.academia.edu/BHoladay?f_ri=1444","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":588,"name":"Nursing","url":"https://www.academia.edu/Documents/in/Nursing?f_ri=1444","nofollow":true},{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true},{"id":11801,"name":"Environmental Monitoring","url":"https://www.academia.edu/Documents/in/Environmental_Monitoring?f_ri=1444","nofollow":true},{"id":16288,"name":"Public Health","url":"https://www.academia.edu/Documents/in/Public_Health?f_ri=1444","nofollow":true},{"id":64933,"name":"Child","url":"https://www.academia.edu/Documents/in/Child?f_ri=1444"},{"id":112651,"name":"San Francisco","url":"https://www.academia.edu/Documents/in/San_Francisco?f_ri=1444"},{"id":134346,"name":"Infant","url":"https://www.academia.edu/Documents/in/Infant?f_ri=1444"},{"id":180470,"name":"Enterobacteriaceae","url":"https://www.academia.edu/Documents/in/Enterobacteriaceae?f_ri=1444"},{"id":203549,"name":"Public Health Nursing","url":"https://www.academia.edu/Documents/in/Public_Health_Nursing?f_ri=1444"},{"id":410370,"name":"Public health systems and services research","url":"https://www.academia.edu/Documents/in/Public_health_systems_and_services_research-1?f_ri=1444"},{"id":958594,"name":"Fecal Coliform","url":"https://www.academia.edu/Documents/in/Fecal_Coliform?f_ri=1444"},{"id":2489700,"name":"Child preschool","url":"https://www.academia.edu/Documents/in/Child_preschool?f_ri=1444"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_26365232" data-work_id="26365232" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/26365232/McpQ_is_a_specific_citrate_chemoreceptor_that_responds_preferentially_to_citrate_metal_ion_complexes">McpQ is a specific citrate chemoreceptor that responds preferentially to citrate/metal ion complexes</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Chemoreceptors are at the beginning of chemosensory pathways that mediate chemotaxis. Pseudomonas putida KT2440 is predicted to have 27 chemoreceptors, most of which uncharacterised. We have previously identified McpS as chemoreceptor for... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_26365232" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Chemoreceptors are at the beginning of chemosensory pathways that mediate chemotaxis. Pseudomonas putida KT2440 is predicted to have 27 chemoreceptors, most of which uncharacterised. We have previously identified McpS as chemoreceptor for Krebs cycle intermediates. Citrate is primarily present in the environment as metalcomplex, which, however, is not recognized by McpS. We show here that the McpS paralogue McpQ recognizes specifically citrate and citrate/metal 2+ complexes. The McpQ ligand binding domain (McpQ-LBD) binds citrate/metal 2+ complexes with higher affinity than citrate. McpQ-LBD is present in a monomer-dimer equilibrium and citrate and particularly citrate/Mg 2+ binding stabilize the dimer. The bacterium showed much stronger responses to citrate/Mg 2+ than to citrate and mcpQ inactivation caused a dramatic reduction in chemotaxis. Responses to Krebs cycle intermediates are thus mediated by the broad range McpS and McpQ that responds specifically to an intermediate not recognized by McpS. Interesting parallels exist to the paralogous amino acid chemoreceptors of P. aeruginosa and Bacillus subtilis. Whereas one paralogue recognizes most amino acids, the remaining paralogue binds specifically one of the few acids not recognized by the broad range receptors. Therefore, chemotaxis to compound families by the concerted action of broad and narrow range receptors may represent a general mechanism.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/26365232" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="0c421f0a8100702c6b209a3871a85f7e" rel="nofollow" data-download="{"attachment_id":46671621,"asset_id":26365232,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/46671621/download_file?st=MTc0MDU3NTMzMyw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="32857520" href="https://csic.academia.edu/TinoKrell">Tino Krell</a><script data-card-contents-for-user="32857520" type="text/json">{"id":32857520,"first_name":"Tino","last_name":"Krell","domain_name":"csic","page_name":"TinoKrell","display_name":"Tino Krell","profile_url":"https://csic.academia.edu/TinoKrell?f_ri=1444","photo":"https://0.academia-photos.com/32857520/13224633/14507152/s65_tino.krell.jpg"}</script></span></span></li><li class="js-paper-rank-work_26365232 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="26365232"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 26365232, container: ".js-paper-rank-work_26365232", }); });</script></li><li class="js-percentile-work_26365232 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 26365232; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_26365232"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_26365232 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="26365232"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 26365232; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=26365232]").text(description); $(".js-view-count-work_26365232").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_26365232").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="26365232"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">2</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="159" rel="nofollow" href="https://www.academia.edu/Documents/in/Microbiology">Microbiology</a>, <script data-card-contents-for-ri="159" type="text/json">{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="1444" rel="nofollow" href="https://www.academia.edu/Documents/in/Environmental_microbiology">Environmental microbiology</a><script data-card-contents-for-ri="1444" type="text/json">{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=26365232]'), work: {"id":26365232,"title":"McpQ is a specific citrate chemoreceptor that responds preferentially to citrate/metal ion complexes","created_at":"2016-06-21T04:33:21.068-07:00","url":"https://www.academia.edu/26365232/McpQ_is_a_specific_citrate_chemoreceptor_that_responds_preferentially_to_citrate_metal_ion_complexes?f_ri=1444","dom_id":"work_26365232","summary":"Chemoreceptors are at the beginning of chemosensory pathways that mediate chemotaxis. Pseudomonas putida KT2440 is predicted to have 27 chemoreceptors, most of which uncharacterised. We have previously identified McpS as chemoreceptor for Krebs cycle intermediates. Citrate is primarily present in the environment as metalcomplex, which, however, is not recognized by McpS. We show here that the McpS paralogue McpQ recognizes specifically citrate and citrate/metal 2+ complexes. The McpQ ligand binding domain (McpQ-LBD) binds citrate/metal 2+ complexes with higher affinity than citrate. McpQ-LBD is present in a monomer-dimer equilibrium and citrate and particularly citrate/Mg 2+ binding stabilize the dimer. The bacterium showed much stronger responses to citrate/Mg 2+ than to citrate and mcpQ inactivation caused a dramatic reduction in chemotaxis. Responses to Krebs cycle intermediates are thus mediated by the broad range McpS and McpQ that responds specifically to an intermediate not recognized by McpS. Interesting parallels exist to the paralogous amino acid chemoreceptors of P. aeruginosa and Bacillus subtilis. Whereas one paralogue recognizes most amino acids, the remaining paralogue binds specifically one of the few acids not recognized by the broad range receptors. Therefore, chemotaxis to compound families by the concerted action of broad and narrow range receptors may represent a general mechanism.","downloadable_attachments":[{"id":46671621,"asset_id":26365232,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":32857520,"first_name":"Tino","last_name":"Krell","domain_name":"csic","page_name":"TinoKrell","display_name":"Tino Krell","profile_url":"https://csic.academia.edu/TinoKrell?f_ri=1444","photo":"https://0.academia-photos.com/32857520/13224633/14507152/s65_tino.krell.jpg"}],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=1444","nofollow":true},{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_12758046 coauthored" data-work_id="12758046" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/12758046/Deep_sea_bacteria_enriched_by_oil_and_dispersant_from_the_Deepwater_Horizon_spill">Deep-sea bacteria enriched by oil and dispersant from the Deepwater Horizon spill</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">The Deepwater Horizon oil spill in the Gulf of Mexico resulted in a deep-sea hydrocarbon plume that caused a shift in the indigenous microbial community composition with unknown ecological consequences. Early in the spill history, a bloom... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_12758046" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">The Deepwater Horizon oil spill in the Gulf of Mexico resulted in a deep-sea hydrocarbon plume that caused a shift in the indigenous microbial community composition with unknown ecological consequences. Early in the spill history, a bloom of uncultured, thus uncharacterized, members of the Oceanospirillales was previously detected, but their role in oil disposition was unknown. Here our aim was to determine the functional role of the Oceanospirillales and other active members of the indigenous microbial community using deep sequencing of community DNA and RNA, as well as single-cell genomics. Shotgun metagenomic and metatranscriptomic sequencing revealed that genes for motility, chemotaxis and aliphatic hydrocarbon degradation were significantly enriched and expressed in the hydrocarbon plume samples compared with uncontaminated seawater collected from plume depth. In contrast, although genes coding for degradation of more recalcitrant compounds, such as benzene, toluene, ethylbenzene, total xylenes and polycyclic aromatic hydrocarbons, were identified in the metagenomes, they were expressed at low levels, or not at all based on analysis of the metatranscriptomes. Isolation and sequencing of two Oceanospirillales single cells revealed that both cells possessed genes coding for n-alkane and cycloalkane degradation. Specifically, the near-complete pathway for cyclohexane oxidation in the Oceanospirillales single cells was elucidated and supported by both metagenome and metatranscriptome data. The draft genome also included genes for chemotaxis, motility and nutrient acquisition strategies that were also identified in the metagenomes and metatranscriptomes. These data point towards a rapid response of members of the Oceanospirillales to aliphatic hydrocarbons in the deep sea.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/12758046" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="e7c92cfae229e388c5d651dbfe0986e2" rel="nofollow" data-download="{"attachment_id":45957708,"asset_id":12758046,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/45957708/download_file?st=MTc0MDU3NTMzMyw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="35356544" href="https://independent.academia.edu/MarcinZemla">Marcin Zemla</a><script data-card-contents-for-user="35356544" type="text/json">{"id":35356544,"first_name":"Marcin","last_name":"Zemla","domain_name":"independent","page_name":"MarcinZemla","display_name":"Marcin Zemla","profile_url":"https://independent.academia.edu/MarcinZemla?f_ri=1444","photo":"/images/s65_no_pic.png"}</script></span></span><span class="u-displayInlineBlock InlineList-item-text"> and <span class="u-textDecorationUnderline u-clickable InlineList-item-text js-work-more-authors-12758046">+4</span><div class="hidden js-additional-users-12758046"><div><span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a href="https://independent.academia.edu/SBorglin">S. Borglin</a></span></div><div><span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a href="https://lbl.academia.edu/MBill">M. Bill</a></span></div><div><span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a href="https://lbl.academia.edu/SusannahTringe">Susannah Tringe</a></span></div><div><span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a href="https://lbl.academia.edu/HoiYingHolman">Hoi-Ying Holman</a></span></div></div></span><script>(function(){ var popoverSettings = { el: $('.js-work-more-authors-12758046'), placement: 'bottom', hide_delay: 200, html: true, content: function(){ return $('.js-additional-users-12758046').html(); } } new HoverPopover(popoverSettings); })();</script></li><li class="js-paper-rank-work_12758046 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="12758046"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 12758046, container: ".js-paper-rank-work_12758046", }); });</script></li><li class="js-percentile-work_12758046 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 12758046; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_12758046"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_12758046 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="12758046"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 12758046; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=12758046]").text(description); $(".js-view-count-work_12758046").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_12758046").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="12758046"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">9</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="159" rel="nofollow" href="https://www.academia.edu/Documents/in/Microbiology">Microbiology</a>, <script data-card-contents-for-ri="159" type="text/json">{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="1444" rel="nofollow" href="https://www.academia.edu/Documents/in/Environmental_microbiology">Environmental microbiology</a>, <script data-card-contents-for-ri="1444" type="text/json">{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="5285" rel="nofollow" href="https://www.academia.edu/Documents/in/Petroleum">Petroleum</a>, <script data-card-contents-for-ri="5285" type="text/json">{"id":5285,"name":"Petroleum","url":"https://www.academia.edu/Documents/in/Petroleum?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="52055" rel="nofollow" href="https://www.academia.edu/Documents/in/Lipids">Lipids</a><script data-card-contents-for-ri="52055" type="text/json">{"id":52055,"name":"Lipids","url":"https://www.academia.edu/Documents/in/Lipids?f_ri=1444","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=12758046]'), work: {"id":12758046,"title":"Deep-sea bacteria enriched by oil and dispersant from the Deepwater Horizon spill","created_at":"2015-06-02T13:06:38.191-07:00","url":"https://www.academia.edu/12758046/Deep_sea_bacteria_enriched_by_oil_and_dispersant_from_the_Deepwater_Horizon_spill?f_ri=1444","dom_id":"work_12758046","summary":"The Deepwater Horizon oil spill in the Gulf of Mexico resulted in a deep-sea hydrocarbon plume that caused a shift in the indigenous microbial community composition with unknown ecological consequences. Early in the spill history, a bloom of uncultured, thus uncharacterized, members of the Oceanospirillales was previously detected, but their role in oil disposition was unknown. Here our aim was to determine the functional role of the Oceanospirillales and other active members of the indigenous microbial community using deep sequencing of community DNA and RNA, as well as single-cell genomics. Shotgun metagenomic and metatranscriptomic sequencing revealed that genes for motility, chemotaxis and aliphatic hydrocarbon degradation were significantly enriched and expressed in the hydrocarbon plume samples compared with uncontaminated seawater collected from plume depth. In contrast, although genes coding for degradation of more recalcitrant compounds, such as benzene, toluene, ethylbenzene, total xylenes and polycyclic aromatic hydrocarbons, were identified in the metagenomes, they were expressed at low levels, or not at all based on analysis of the metatranscriptomes. Isolation and sequencing of two Oceanospirillales single cells revealed that both cells possessed genes coding for n-alkane and cycloalkane degradation. Specifically, the near-complete pathway for cyclohexane oxidation in the Oceanospirillales single cells was elucidated and supported by both metagenome and metatranscriptome data. The draft genome also included genes for chemotaxis, motility and nutrient acquisition strategies that were also identified in the metagenomes and metatranscriptomes. These data point towards a rapid response of members of the Oceanospirillales to aliphatic hydrocarbons in the deep sea.","downloadable_attachments":[{"id":45957708,"asset_id":12758046,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":35356544,"first_name":"Marcin","last_name":"Zemla","domain_name":"independent","page_name":"MarcinZemla","display_name":"Marcin Zemla","profile_url":"https://independent.academia.edu/MarcinZemla?f_ri=1444","photo":"/images/s65_no_pic.png"},{"id":31845430,"first_name":"S.","last_name":"Borglin","domain_name":"independent","page_name":"SBorglin","display_name":"S. Borglin","profile_url":"https://independent.academia.edu/SBorglin?f_ri=1444","photo":"/images/s65_no_pic.png"},{"id":31803052,"first_name":"M.","last_name":"Bill","domain_name":"lbl","page_name":"MBill","display_name":"M. Bill","profile_url":"https://lbl.academia.edu/MBill?f_ri=1444","photo":"https://0.academia-photos.com/31803052/18659471/18619606/s65_m..bill.jpg"},{"id":31658155,"first_name":"Susannah","last_name":"Tringe","domain_name":"lbl","page_name":"SusannahTringe","display_name":"Susannah Tringe","profile_url":"https://lbl.academia.edu/SusannahTringe?f_ri=1444","photo":"/images/s65_no_pic.png"},{"id":3954039,"first_name":"Hoi-Ying","last_name":"Holman","domain_name":"lbl","page_name":"HoiYingHolman","display_name":"Hoi-Ying Holman","profile_url":"https://lbl.academia.edu/HoiYingHolman?f_ri=1444","photo":"https://0.academia-photos.com/3954039/115837626/105127302/s65_hoi-ying.holman.jpeg"}],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=1444","nofollow":true},{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true},{"id":5285,"name":"Petroleum","url":"https://www.academia.edu/Documents/in/Petroleum?f_ri=1444","nofollow":true},{"id":52055,"name":"Lipids","url":"https://www.academia.edu/Documents/in/Lipids?f_ri=1444","nofollow":true},{"id":113903,"name":"Bacteria","url":"https://www.academia.edu/Documents/in/Bacteria?f_ri=1444"},{"id":170384,"name":"Gulf War","url":"https://www.academia.edu/Documents/in/Gulf_War?f_ri=1444"},{"id":547547,"name":"Bacterial Load","url":"https://www.academia.edu/Documents/in/Bacterial_Load?f_ri=1444"},{"id":646441,"name":"Water Microbiology","url":"https://www.academia.edu/Documents/in/Water_Microbiology?f_ri=1444"},{"id":815324,"name":"Petroleum Pollution","url":"https://www.academia.edu/Documents/in/Petroleum_Pollution?f_ri=1444"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_14062647" data-work_id="14062647" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/14062647/Multiplex_PCR_for_Detection_of_Botulinum_Neurotoxin_Producing_Clostridia_in_Clinical_Food_and_Environmental_Samples">Multiplex PCR for Detection of Botulinum Neurotoxin-Producing Clostridia in Clinical, Food, and Environmental Samples</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Botulinum neurotoxin (BoNT), the most toxic substance known, is produced by the spore-forming bacterium Clostridium botulinum and, in rare cases, also by some strains of Clostridium butyricum and Clostridium baratii. The standard... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_14062647" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Botulinum neurotoxin (BoNT), the most toxic substance known, is produced by the spore-forming bacterium Clostridium botulinum and, in rare cases, also by some strains of Clostridium butyricum and Clostridium baratii. The standard procedure for definitive detection of BoNT-producing clostridia is a culture method combined with neurotoxin detection using a standard mouse bioassay (SMB). The SMB is highly sensitive and specific, but it is expensive and time-consuming and there are ethical concerns due to use of laboratory animals. PCR provides a rapid alternative for initial screening for BoNT-producing clostridia. In this study, a previously described multiplex PCR assay was modified to detect all type A, B, E, and F neurotoxin genes in isolated strains and in clinical, food, environmental samples. This assay includes an internal amplification control. The effectiveness of the multiplex PCR method for detecting clostridia possessing type A, B, E, and F neurotoxin genes was evaluated by direct comparison with the SMB. This method showed 100% inclusivity and 100% exclusivity when 182 BoNT-producing clostridia and 21 other bacterial strains were used. The relative accuracy of the multiplex PCR and SMB was evaluated using 532 clinical, food, and environmental samples and was estimated to be 99.2%. The multiplex PCR was also used to investigate 110 freshly collected food and environmental samples, and 4 of the 110 samples (3.6%) were positive for BoNT-encoding genes.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/14062647" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="370a38d79bf1e9ac685df755ae191357" rel="nofollow" data-download="{"attachment_id":44646203,"asset_id":14062647,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/44646203/download_file?st=MTc0MDU3NTMzMyw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="33080449" href="https://iss-it.academia.edu/DarioMedici">Dario Medici</a><script data-card-contents-for-user="33080449" type="text/json">{"id":33080449,"first_name":"Dario","last_name":"Medici","domain_name":"iss-it","page_name":"DarioMedici","display_name":"Dario Medici","profile_url":"https://iss-it.academia.edu/DarioMedici?f_ri=1444","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_14062647 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="14062647"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 14062647, container: ".js-paper-rank-work_14062647", }); 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The standard procedure for definitive detection of BoNT-producing clostridia is a culture method combined with neurotoxin detection using a standard mouse bioassay (SMB). The SMB is highly sensitive and specific, but it is expensive and time-consuming and there are ethical concerns due to use of laboratory animals. PCR provides a rapid alternative for initial screening for BoNT-producing clostridia. In this study, a previously described multiplex PCR assay was modified to detect all type A, B, E, and F neurotoxin genes in isolated strains and in clinical, food, environmental samples. This assay includes an internal amplification control. The effectiveness of the multiplex PCR method for detecting clostridia possessing type A, B, E, and F neurotoxin genes was evaluated by direct comparison with the SMB. This method showed 100% inclusivity and 100% exclusivity when 182 BoNT-producing clostridia and 21 other bacterial strains were used. The relative accuracy of the multiplex PCR and SMB was evaluated using 532 clinical, food, and environmental samples and was estimated to be 99.2%. The multiplex PCR was also used to investigate 110 freshly collected food and environmental samples, and 4 of the 110 samples (3.6%) were positive for BoNT-encoding genes.","downloadable_attachments":[{"id":44646203,"asset_id":14062647,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":33080449,"first_name":"Dario","last_name":"Medici","domain_name":"iss-it","page_name":"DarioMedici","display_name":"Dario Medici","profile_url":"https://iss-it.academia.edu/DarioMedici?f_ri=1444","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true},{"id":28235,"name":"Multidisciplinary","url":"https://www.academia.edu/Documents/in/Multidisciplinary?f_ri=1444","nofollow":true},{"id":31632,"name":"Food Microbiology","url":"https://www.academia.edu/Documents/in/Food_Microbiology?f_ri=1444","nofollow":true},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice?f_ri=1444","nofollow":true},{"id":96502,"name":"Applied","url":"https://www.academia.edu/Documents/in/Applied?f_ri=1444"},{"id":100290,"name":"Laboratory Animals","url":"https://www.academia.edu/Documents/in/Laboratory_Animals?f_ri=1444"},{"id":107664,"name":"Applied Environmental Microbiology","url":"https://www.academia.edu/Documents/in/Applied_Environmental_Microbiology?f_ri=1444"},{"id":118339,"name":"Polymerase Chain Reaction","url":"https://www.academia.edu/Documents/in/Polymerase_Chain_Reaction?f_ri=1444"},{"id":191520,"name":"multiplex PCR","url":"https://www.academia.edu/Documents/in/multiplex_PCR?f_ri=1444"},{"id":239480,"name":"Neurotoxins","url":"https://www.academia.edu/Documents/in/Neurotoxins?f_ri=1444"},{"id":809882,"name":"Base Sequence","url":"https://www.academia.edu/Documents/in/Base_Sequence?f_ri=1444"},{"id":901876,"name":"Sensitivity and Specificity","url":"https://www.academia.edu/Documents/in/Sensitivity_and_Specificity?f_ri=1444"},{"id":1109769,"name":"Biological Assay","url":"https://www.academia.edu/Documents/in/Biological_Assay?f_ri=1444"},{"id":1292672,"name":"Clostridium","url":"https://www.academia.edu/Documents/in/Clostridium?f_ri=1444"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_21368350" data-work_id="21368350" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/21368350/Songbirds_as_general_transmitters_but_selective_amplifiers_of_B_orrelia_burgdorferi_sensu_lato_genotypes_in_I_xodes_rinicus_ticks">Songbirds as general transmitters but selective amplifiers of B orrelia burgdorferi sensu lato genotypes in I xodes rinicus ticks</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Borrelia burgdorferi s.l. bacteria. Borrelia-naïve birds were recurrently exposed to Ixodes ricinus nymphs carrying a community of more than 34 5S-23S genotypes belonging to five genospecies (Borrelia garinii, Borrelia valaisiana,... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_21368350" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Borrelia burgdorferi s.l. bacteria. Borrelia-naïve birds were recurrently exposed to Ixodes ricinus nymphs carrying a community of more than 34 5S-23S genotypes belonging to five genospecies (Borrelia garinii, Borrelia valaisiana, Borrelia afzelii, B. burgdorferi s.s. and Borrelia spielmanii). Fed ticks were screened for Borrelia after moulting. We found evidence for co-feeding transmission of avian and possibly also mammalian genotypes. Throughout the course of infestations, the infection rate of B. garinii and B. valaisiana increased, indicating successful amplification and transmission, while the infection rate for B. afzelii, B. burgdorferi s.s and B. spielmanii tended to decrease. Within the B. garinii and B. valaisiana genotype communities, certain genotypes were transmitted more than others. Moreover, birds were able to host mixed infections of B. garinii and B. valaisiana, as well as mixed infections of genotypes of the same genospecies. We experimentally show that resident songbirds transmit a broad range of Borrelia genotypes, but selectively amplify certain genotypes, and that one bird can transmit simultaneously several genotypes. Our results highlight the need to explicitly consider the association between genotypes and hosts, which may offer opportunities to point out which hosts are most responsible for the Borrelia presence in questing ticks.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/21368350" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="b10c8d85919449f097e4847c75fe87bd" rel="nofollow" data-download="{"attachment_id":41837159,"asset_id":21368350,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/41837159/download_file?st=MTc0MDU3NTMzMyw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="42452068" href="https://independent.academia.edu/ManojFonville">Manoj Fonville</a><script data-card-contents-for-user="42452068" type="text/json">{"id":42452068,"first_name":"Manoj","last_name":"Fonville","domain_name":"independent","page_name":"ManojFonville","display_name":"Manoj Fonville","profile_url":"https://independent.academia.edu/ManojFonville?f_ri=1444","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_21368350 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="21368350"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 21368350, container: ".js-paper-rank-work_21368350", }); 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Borrelia-naïve birds were recurrently exposed to Ixodes ricinus nymphs carrying a community of more than 34 5S-23S genotypes belonging to five genospecies (Borrelia garinii, Borrelia valaisiana, Borrelia afzelii, B. burgdorferi s.s. and Borrelia spielmanii). Fed ticks were screened for Borrelia after moulting. We found evidence for co-feeding transmission of avian and possibly also mammalian genotypes. Throughout the course of infestations, the infection rate of B. garinii and B. valaisiana increased, indicating successful amplification and transmission, while the infection rate for B. afzelii, B. burgdorferi s.s and B. spielmanii tended to decrease. Within the B. garinii and B. valaisiana genotype communities, certain genotypes were transmitted more than others. Moreover, birds were able to host mixed infections of B. garinii and B. valaisiana, as well as mixed infections of genotypes of the same genospecies. We experimentally show that resident songbirds transmit a broad range of Borrelia genotypes, but selectively amplify certain genotypes, and that one bird can transmit simultaneously several genotypes. Our results highlight the need to explicitly consider the association between genotypes and hosts, which may offer opportunities to point out which hosts are most responsible for the Borrelia presence in questing ticks.","downloadable_attachments":[{"id":41837159,"asset_id":21368350,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":42452068,"first_name":"Manoj","last_name":"Fonville","domain_name":"independent","page_name":"ManojFonville","display_name":"Manoj Fonville","profile_url":"https://independent.academia.edu/ManojFonville?f_ri=1444","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=1444","nofollow":true},{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true},{"id":53198,"name":"Lyme disease","url":"https://www.academia.edu/Documents/in/Lyme_disease?f_ri=1444","nofollow":true},{"id":151693,"name":"Songbirds","url":"https://www.academia.edu/Documents/in/Songbirds?f_ri=1444","nofollow":true},{"id":372410,"name":"Genotype","url":"https://www.academia.edu/Documents/in/Genotype?f_ri=1444"},{"id":756265,"name":"Borrelia","url":"https://www.academia.edu/Documents/in/Borrelia?f_ri=1444"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_1466610" data-work_id="1466610" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/1466610/ALIMENTOS_EN_EL_CAMBIO_CLIMATICO_Efectos_en_la_Salud">ALIMENTOS EN EL CAMBIO CLIMATICO. 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This phage has deployed lytic activity against the Enterobacter cloacae complex, a... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_79010721" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Here, we present the complete genome sequence of Enterobacter phage vB_EcRAM-01, isolated from waters of the Río Abajo river, in Panama City, Panama. This phage has deployed lytic activity against the Enterobacter cloacae complex, a pathogen of clinical importance in intensive care units. It belongs to the Myoviridae family and has a double-stranded DNA genome that is 178,477 bp long and contains 293 open reading frames (ORFs).</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/79010721" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="181ad62df3d81c828c3ba034805d23f7" rel="nofollow" data-download="{"attachment_id":85875258,"asset_id":79010721,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/85875258/download_file?st=MTc0MDU3NTMzMyw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="41092113" href="https://uba.academia.edu/EdnnerEmmanuelVictoriaBlanco">Ednner Emmanuel Victoria-Blanco</a><script data-card-contents-for-user="41092113" type="text/json">{"id":41092113,"first_name":"Ednner Emmanuel","last_name":"Victoria-Blanco","domain_name":"uba","page_name":"EdnnerEmmanuelVictoriaBlanco","display_name":"Ednner Emmanuel Victoria-Blanco","profile_url":"https://uba.academia.edu/EdnnerEmmanuelVictoriaBlanco?f_ri=1444","photo":"https://0.academia-photos.com/41092113/12310440/13705869/s65_ednner.victoria_blanco.jpg_oh_8ce1216307e6c004f2519ce156ba9241_oe_5780ca42"}</script></span></span></li><li class="js-paper-rank-work_79010721 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="79010721"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 79010721, container: ".js-paper-rank-work_79010721", }); 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This phage has deployed lytic activity against the Enterobacter cloacae complex, a pathogen of clinical importance in intensive care units. It belongs to the Myoviridae family and has a double-stranded DNA genome that is 178,477 bp long and contains 293 open reading frames (ORFs).","downloadable_attachments":[{"id":85875258,"asset_id":79010721,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":41092113,"first_name":"Ednner Emmanuel","last_name":"Victoria-Blanco","domain_name":"uba","page_name":"EdnnerEmmanuelVictoriaBlanco","display_name":"Ednner Emmanuel Victoria-Blanco","profile_url":"https://uba.academia.edu/EdnnerEmmanuelVictoriaBlanco?f_ri=1444","photo":"https://0.academia-photos.com/41092113/12310440/13705869/s65_ednner.victoria_blanco.jpg_oh_8ce1216307e6c004f2519ce156ba9241_oe_5780ca42"}],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=1444","nofollow":true},{"id":170,"name":"Molecular Virology (Biology)","url":"https://www.academia.edu/Documents/in/Molecular_Virology_Biology_?f_ri=1444","nofollow":true},{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true},{"id":3216,"name":"Genomics","url":"https://www.academia.edu/Documents/in/Genomics?f_ri=1444","nofollow":true},{"id":8089,"name":"Virology","url":"https://www.academia.edu/Documents/in/Virology?f_ri=1444"},{"id":166418,"name":"Phages","url":"https://www.academia.edu/Documents/in/Phages?f_ri=1444"},{"id":183098,"name":"Phage Therapy","url":"https://www.academia.edu/Documents/in/Phage_Therapy?f_ri=1444"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_71752554" data-work_id="71752554" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a 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u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/68436740/Susceptibility_of_Microsporum_canis_arthrospores_to_a_mixture_of_chemically_defined_essential_oils_a_perspective_for_environmental_decontamination">Susceptibility of Microsporum canis arthrospores to a mixture of chemically defined essential oils: a perspective for environmental decontamination</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">The zoophilic dermatophyte Microsporum canis has cats as natural reservoir, but it is able to infect a wide range of hosts, including humans, where different clinical features of the so-called ringworm dermatophytosis have been described.... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_68436740" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">The zoophilic dermatophyte Microsporum canis has cats as natural reservoir, but it is able to infect a wide range of hosts, including humans, where different clinical features of the so-called ringworm dermatophytosis have been described. Human infections are increasingly been reported in Mediterranean countries. A reliable control program against M. canis infection in cats should include an antifungal treatment of both the infected animals and their living environment. In this article, a herbal mixture composed of chemically defined essential oils (EOs) of Litsea cubeba (1%), Illicium verum, Foeniculum vulgare, and Pelargonium graveolens (0.5% each) was formulated and its antifungal activity assessed against M. canis arthrospores which represent the infective environmental stage of M. canis. Single compounds present in higher amounts in the mixture were also separately tested in vitro. Litsea cubeba and P. graveolens EOs were most effective (minimum inhibitory concentration (MIC) 0...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/68436740" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="ffbab1e5f54dd7f52d980c4850434acb" rel="nofollow" data-download="{"attachment_id":78911356,"asset_id":68436740,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/78911356/download_file?st=MTc0MDU3NTMzMyw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="33681735" href="https://independent.academia.edu/LuisaPistelli">Luisa Pistelli</a><script data-card-contents-for-user="33681735" type="text/json">{"id":33681735,"first_name":"Luisa","last_name":"Pistelli","domain_name":"independent","page_name":"LuisaPistelli","display_name":"Luisa Pistelli","profile_url":"https://independent.academia.edu/LuisaPistelli?f_ri=1444","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_68436740 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="68436740"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 68436740, container: ".js-paper-rank-work_68436740", }); 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Human infections are increasingly been reported in Mediterranean countries. A reliable control program against M. canis infection in cats should include an antifungal treatment of both the infected animals and their living environment. In this article, a herbal mixture composed of chemically defined essential oils (EOs) of Litsea cubeba (1%), Illicium verum, Foeniculum vulgare, and Pelargonium graveolens (0.5% each) was formulated and its antifungal activity assessed against M. canis arthrospores which represent the infective environmental stage of M. canis. Single compounds present in higher amounts in the mixture were also separately tested in vitro. Litsea cubeba and P. graveolens EOs were most effective (minimum inhibitory concentration (MIC) 0...","downloadable_attachments":[{"id":78911356,"asset_id":68436740,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":33681735,"first_name":"Luisa","last_name":"Pistelli","domain_name":"independent","page_name":"LuisaPistelli","display_name":"Luisa Pistelli","profile_url":"https://independent.academia.edu/LuisaPistelli?f_ri=1444","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true},{"id":19080,"name":"Zoonoses","url":"https://www.academia.edu/Documents/in/Zoonoses?f_ri=1444","nofollow":true},{"id":41088,"name":"Cats","url":"https://www.academia.edu/Documents/in/Cats?f_ri=1444","nofollow":true},{"id":83996,"name":"Hair","url":"https://www.academia.edu/Documents/in/Hair?f_ri=1444","nofollow":true},{"id":373754,"name":"Ecosystem","url":"https://www.academia.edu/Documents/in/Ecosystem?f_ri=1444"},{"id":552209,"name":"Antifungal Agents","url":"https://www.academia.edu/Documents/in/Antifungal_Agents?f_ri=1444"},{"id":561014,"name":"Microbial Sensitivity Tests","url":"https://www.academia.edu/Documents/in/Microbial_Sensitivity_Tests?f_ri=1444"},{"id":957423,"name":"Decontamination","url":"https://www.academia.edu/Documents/in/Decontamination?f_ri=1444"},{"id":1441864,"name":"Microsporum","url":"https://www.academia.edu/Documents/in/Microsporum?f_ri=1444"},{"id":3789884,"name":"Pharmacology and pharmaceutical sciences","url":"https://www.academia.edu/Documents/in/Pharmacology_and_pharmaceutical_sciences?f_ri=1444"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_63306089" data-work_id="63306089" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/63306089/Bacteriophage_Lambda_A_Paradigm_Revisited">Bacteriophage Lambda – A Paradigm Revisited</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Bacteriophage lambda has an archetypal immunity system, which prevents superinfection of its E. coli lysogens. It is now known that superinfection at high frequency can occur with toxigenic lambda-like phages, and here we demonstrate that... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_63306089" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Bacteriophage lambda has an archetypal immunity system, which prevents superinfection of its E. coli lysogens. It is now known that superinfection at high frequency can occur with toxigenic lambda-like phages, and here we demonstrate that superinfection of a lambda lysogen can lead to acquisition of additional lambda genomes, confirmed by Southern hybridisation and quantitative PCR. As many as 8 integration events were observed, but at very low frequency (6.4 x 10(-4)) and always as multiple insertions at the established primary integration site in E. coli. Sequence analysis of the complete immunity region demonstrated that these multiply infected lysogens were not immunity mutants. In conclusion, although lambda superinfection immunity can be confounded, it is a rare event.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/63306089" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="6c39721559b5fdbb09eecbd06b7edfb0" rel="nofollow" data-download="{"attachment_id":75780126,"asset_id":63306089,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/75780126/download_file?st=MTc0MDU3NTMzMyw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="136525" href="https://liverpool.academia.edu/HeatherAllison">Heather Allison</a><script data-card-contents-for-user="136525" type="text/json">{"id":136525,"first_name":"Heather","last_name":"Allison","domain_name":"liverpool","page_name":"HeatherAllison","display_name":"Heather Allison","profile_url":"https://liverpool.academia.edu/HeatherAllison?f_ri=1444","photo":"https://0.academia-photos.com/136525/36223/299929/s65_heather.allison.jpg"}</script></span></span></li><li class="js-paper-rank-work_63306089 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="63306089"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 63306089, container: ".js-paper-rank-work_63306089", }); 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It is now known that superinfection at high frequency can occur with toxigenic lambda-like phages, and here we demonstrate that superinfection of a lambda lysogen can lead to acquisition of additional lambda genomes, confirmed by Southern hybridisation and quantitative PCR. As many as 8 integration events were observed, but at very low frequency (6.4 x 10(-4)) and always as multiple insertions at the established primary integration site in E. coli. Sequence analysis of the complete immunity region demonstrated that these multiply infected lysogens were not immunity mutants. In conclusion, although lambda superinfection immunity can be confounded, it is a rare event.","downloadable_attachments":[{"id":75780126,"asset_id":63306089,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":136525,"first_name":"Heather","last_name":"Allison","domain_name":"liverpool","page_name":"HeatherAllison","display_name":"Heather Allison","profile_url":"https://liverpool.academia.edu/HeatherAllison?f_ri=1444","photo":"https://0.academia-photos.com/136525/36223/299929/s65_heather.allison.jpg"}],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=1444","nofollow":true},{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true},{"id":2513,"name":"Molecular Biology","url":"https://www.academia.edu/Documents/in/Molecular_Biology?f_ri=1444","nofollow":true},{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology?f_ri=1444","nofollow":true},{"id":8089,"name":"Virology","url":"https://www.academia.edu/Documents/in/Virology?f_ri=1444"},{"id":8090,"name":"Bacteriophages","url":"https://www.academia.edu/Documents/in/Bacteriophages?f_ri=1444"},{"id":13744,"name":"Molecular Microbiology","url":"https://www.academia.edu/Documents/in/Molecular_Microbiology?f_ri=1444"},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=1444"},{"id":82145,"name":"Virus","url":"https://www.academia.edu/Documents/in/Virus?f_ri=1444"},{"id":83128,"name":"Escherichia coli","url":"https://www.academia.edu/Documents/in/Escherichia_coli?f_ri=1444"},{"id":118339,"name":"Polymerase Chain Reaction","url":"https://www.academia.edu/Documents/in/Polymerase_Chain_Reaction?f_ri=1444"},{"id":809881,"name":"Amino Acid Sequence","url":"https://www.academia.edu/Documents/in/Amino_Acid_Sequence?f_ri=1444"},{"id":809882,"name":"Base Sequence","url":"https://www.academia.edu/Documents/in/Base_Sequence?f_ri=1444"},{"id":2223053,"name":"Lysogeny","url":"https://www.academia.edu/Documents/in/Lysogeny?f_ri=1444"},{"id":2467566,"name":"Molecular Sequence Data","url":"https://www.academia.edu/Documents/in/Molecular_Sequence_Data?f_ri=1444"},{"id":2940274,"name":"virus integration","url":"https://www.academia.edu/Documents/in/virus_integration?f_ri=1444"},{"id":3763225,"name":"Medical and Health Sciences","url":"https://www.academia.edu/Documents/in/Medical_and_Health_Sciences?f_ri=1444"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_57067807" data-work_id="57067807" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/57067807/Efficacy_of_Commonly_Used_Disinfectants_for_Inactivation_of_Human_Noroviruses_and_Their_Surrogates">Efficacy of Commonly Used Disinfectants for Inactivation of Human Noroviruses and Their Surrogates</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Human noroviruses (HuNoVs) are the most common cause of acute viral gastroenteritis worldwide and are a leading cause of foodborne disease. Their environmental persistence and purported resistance to disinfection undoubtedly contribute to... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_57067807" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Human noroviruses (HuNoVs) are the most common cause of acute viral gastroenteritis worldwide and are a leading cause of foodborne disease. Their environmental persistence and purported resistance to disinfection undoubtedly contribute to their success as foodborne disease agents. The purpose of this study was to compare the efficacy of three commonly used disinfectant active ingredients against representative HuNoV strains and cultivable surrogates. Ethanol (50, 70, and 90%), sodium hypochlorite (5, 75, 250, 500, and 1,000 ppm), and a quaternary ammonium compound blend (at 0.1×, 1.0×, and 10× concentrations) were evaluated against two norovirus (NoV) genogroup II strains (GII.2 and GII.4) and two surrogates (feline calicivirus [FCV] and murine norovirus [MNV-1]). Virucidal suspension assays (30-s exposure) were conducted in accordance with ASTM International standard E-1052. Virus inactivation was quantified using reverse transcription quantitative PCR targeting the ORFI-ORFII junc...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/57067807" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="978a4a2c6a5d5e55810149960f34878e" rel="nofollow" data-download="{"attachment_id":72145045,"asset_id":57067807,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/72145045/download_file?st=MTc0MDU3NTMzMyw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="32730253" href="https://independent.academia.edu/BaldevGulati">Baldev Gulati</a><script data-card-contents-for-user="32730253" type="text/json">{"id":32730253,"first_name":"Baldev","last_name":"Gulati","domain_name":"independent","page_name":"BaldevGulati","display_name":"Baldev Gulati","profile_url":"https://independent.academia.edu/BaldevGulati?f_ri=1444","photo":"https://0.academia-photos.com/32730253/108228283/97456551/s65_baldev.gulati.jpeg"}</script></span></span></li><li class="js-paper-rank-work_57067807 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="57067807"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 57067807, container: ".js-paper-rank-work_57067807", }); });</script></li><li class="js-percentile-work_57067807 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 57067807; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_57067807"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_57067807 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="57067807"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 57067807; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=57067807]").text(description); $(".js-view-count-work_57067807").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_57067807").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="57067807"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">16</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="1081" rel="nofollow" href="https://www.academia.edu/Documents/in/Food_Safety">Food Safety</a>, <script data-card-contents-for-ri="1081" type="text/json">{"id":1081,"name":"Food Safety","url":"https://www.academia.edu/Documents/in/Food_Safety?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="1444" rel="nofollow" href="https://www.academia.edu/Documents/in/Environmental_microbiology">Environmental microbiology</a>, <script data-card-contents-for-ri="1444" type="text/json">{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="7603" rel="nofollow" href="https://www.academia.edu/Documents/in/Food">Food</a>, <script data-card-contents-for-ri="7603" type="text/json">{"id":7603,"name":"Food","url":"https://www.academia.edu/Documents/in/Food?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="12426" rel="nofollow" href="https://www.academia.edu/Documents/in/Treatment_Outcome">Treatment Outcome</a><script data-card-contents-for-ri="12426" type="text/json">{"id":12426,"name":"Treatment Outcome","url":"https://www.academia.edu/Documents/in/Treatment_Outcome?f_ri=1444","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=57067807]'), work: {"id":57067807,"title":"Efficacy of Commonly Used Disinfectants for Inactivation of Human Noroviruses and Their Surrogates","created_at":"2021-10-10T22:19:11.877-07:00","url":"https://www.academia.edu/57067807/Efficacy_of_Commonly_Used_Disinfectants_for_Inactivation_of_Human_Noroviruses_and_Their_Surrogates?f_ri=1444","dom_id":"work_57067807","summary":"Human noroviruses (HuNoVs) are the most common cause of acute viral gastroenteritis worldwide and are a leading cause of foodborne disease. Their environmental persistence and purported resistance to disinfection undoubtedly contribute to their success as foodborne disease agents. The purpose of this study was to compare the efficacy of three commonly used disinfectant active ingredients against representative HuNoV strains and cultivable surrogates. Ethanol (50, 70, and 90%), sodium hypochlorite (5, 75, 250, 500, and 1,000 ppm), and a quaternary ammonium compound blend (at 0.1×, 1.0×, and 10× concentrations) were evaluated against two norovirus (NoV) genogroup II strains (GII.2 and GII.4) and two surrogates (feline calicivirus [FCV] and murine norovirus [MNV-1]). Virucidal suspension assays (30-s exposure) were conducted in accordance with ASTM International standard E-1052. Virus inactivation was quantified using reverse transcription quantitative PCR targeting the ORFI-ORFII junc...","downloadable_attachments":[{"id":72145045,"asset_id":57067807,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":32730253,"first_name":"Baldev","last_name":"Gulati","domain_name":"independent","page_name":"BaldevGulati","display_name":"Baldev Gulati","profile_url":"https://independent.academia.edu/BaldevGulati?f_ri=1444","photo":"https://0.academia-photos.com/32730253/108228283/97456551/s65_baldev.gulati.jpeg"}],"research_interests":[{"id":1081,"name":"Food Safety","url":"https://www.academia.edu/Documents/in/Food_Safety?f_ri=1444","nofollow":true},{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true},{"id":7603,"name":"Food","url":"https://www.academia.edu/Documents/in/Food?f_ri=1444","nofollow":true},{"id":12426,"name":"Treatment Outcome","url":"https://www.academia.edu/Documents/in/Treatment_Outcome?f_ri=1444","nofollow":true},{"id":28235,"name":"Multidisciplinary","url":"https://www.academia.edu/Documents/in/Multidisciplinary?f_ri=1444"},{"id":78119,"name":"Norovirus","url":"https://www.academia.edu/Documents/in/Norovirus?f_ri=1444"},{"id":121705,"name":"Ethanol","url":"https://www.academia.edu/Documents/in/Ethanol?f_ri=1444"},{"id":122405,"name":"Lettuce","url":"https://www.academia.edu/Documents/in/Lettuce?f_ri=1444"},{"id":128189,"name":"Gastroenteritis","url":"https://www.academia.edu/Documents/in/Gastroenteritis?f_ri=1444"},{"id":139817,"name":"Disinfectants","url":"https://www.academia.edu/Documents/in/Disinfectants?f_ri=1444"},{"id":160814,"name":"Fruit","url":"https://www.academia.edu/Documents/in/Fruit?f_ri=1444"},{"id":221185,"name":"Disinfection","url":"https://www.academia.edu/Documents/in/Disinfection?f_ri=1444"},{"id":342520,"name":"Sodium Hypochlorite","url":"https://www.academia.edu/Documents/in/Sodium_Hypochlorite?f_ri=1444"},{"id":413195,"name":"Time Factors","url":"https://www.academia.edu/Documents/in/Time_Factors?f_ri=1444"},{"id":440924,"name":"Surface Properties","url":"https://www.academia.edu/Documents/in/Surface_Properties?f_ri=1444"},{"id":955937,"name":"Quaternary Ammonium Compounds","url":"https://www.academia.edu/Documents/in/Quaternary_Ammonium_Compounds?f_ri=1444"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_52987093" data-work_id="52987093" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/52987093/Assessment_of_REPLI_g_Multiple_Displacement_Whole_Genome_Amplification_WGA_Techniques_for_Metagenomic_Applications">Assessment of REPLI-g Multiple Displacement Whole Genome Amplification (WGA) Techniques for Metagenomic Applications</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Amplification of minute quantities of DNA is a fundamental challenge in low-biomass metagenomic and microbiome studies because of potential biases in coverage, guanine-cytosine (GC) content, and altered species abundances. Whole genome... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_52987093" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Amplification of minute quantities of DNA is a fundamental challenge in low-biomass metagenomic and microbiome studies because of potential biases in coverage, guanine-cytosine (GC) content, and altered species abundances. Whole genome amplification (WGA), although widely used, is notorious for introducing artifact sequences, either by amplifying laboratory contaminants or by nonrandom amplification of a sample's DNA. In this study, we investigate the effect of REPLI-g multiple displacement amplification (MDA; Qiagen, Valencia, CA, USA) on sequencing data quality and species abundance detection in 8 paired metagenomic samples and 1 titrated, mixed control sample. We extracted and sequenced genomic DNA (gDNA) from 8 environmental samples and compared the quality of the sequencing data for the MDA and their corresponding non-MDA samples. The degree of REPLI-g MDA bias was evaluated by sequence metrics, species composition, and crossvalidating observed species abundance and species diversity estimates using the One Codex and MetaPhlAn taxonomic classification tools. Here, we provide evidence of the overall efficacy of REPLI-g MDA on retaining sequencing data quality and species abundance measurements while providing increased yields of high-fidelity DNA. We find that species abundance estimates are largely consistent across samples, even with REPLI-g amplification, as demonstrated by the Spearman's rank order coefficient (R 2. 0.8). However, REPLI-g MDA often produced fewer classified reads at the species, genera, and family level, resulting in decreased species diversity. We also observed some areas with the PCR "jackpot effect," with varying input DNA values for the Metagenomics Research Group (MGRG) controls at specific genomic loci. We visualize this effect in whole genome coverage plots and with sequence composition analyses and note these caveats of the MDA method. Despite overall concordance of species abundance between the amplified and unamplified samples, these results demonstrate that amplification of DNA using the REPLI-g method has some limitations. These concerns could be addressed by future improvements in the enzymes or methods for REPLI-g to be considered a .99% robust method for increasing the amount of high-fidelity DNA from low-biomass samples or at the very least, accounted for during computational analysis of MDA samples.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/52987093" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="f8d07efb10bf0646b540e9316a8aa652" rel="nofollow" data-download="{"attachment_id":69984220,"asset_id":52987093,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/69984220/download_file?st=MTc0MDU3NTMzMyw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="40860722" href="https://independent.academia.edu/DanielaBezdan">Daniela Bezdan</a><script data-card-contents-for-user="40860722" type="text/json">{"id":40860722,"first_name":"Daniela","last_name":"Bezdan","domain_name":"independent","page_name":"DanielaBezdan","display_name":"Daniela Bezdan","profile_url":"https://independent.academia.edu/DanielaBezdan?f_ri=1444","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_52987093 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="52987093"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 52987093, container: ".js-paper-rank-work_52987093", }); });</script></li><li class="js-percentile-work_52987093 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 52987093; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_52987093"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_52987093 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="52987093"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 52987093; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=52987093]").text(description); $(".js-view-count-work_52987093").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_52987093").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="52987093"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">6</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="923" rel="nofollow" href="https://www.academia.edu/Documents/in/Technology">Technology</a>, <script data-card-contents-for-ri="923" type="text/json">{"id":923,"name":"Technology","url":"https://www.academia.edu/Documents/in/Technology?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="1444" rel="nofollow" href="https://www.academia.edu/Documents/in/Environmental_microbiology">Environmental microbiology</a>, <script data-card-contents-for-ri="1444" type="text/json">{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="21948" rel="nofollow" href="https://www.academia.edu/Documents/in/Metagenomics">Metagenomics</a>, <script data-card-contents-for-ri="21948" type="text/json">{"id":21948,"name":"Metagenomics","url":"https://www.academia.edu/Documents/in/Metagenomics?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="47884" rel="nofollow" href="https://www.academia.edu/Documents/in/Biological_Sciences">Biological Sciences</a><script data-card-contents-for-ri="47884" type="text/json">{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=1444","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=52987093]'), work: {"id":52987093,"title":"Assessment of REPLI-g Multiple Displacement Whole Genome Amplification (WGA) Techniques for Metagenomic Applications","created_at":"2021-09-20T05:46:21.355-07:00","url":"https://www.academia.edu/52987093/Assessment_of_REPLI_g_Multiple_Displacement_Whole_Genome_Amplification_WGA_Techniques_for_Metagenomic_Applications?f_ri=1444","dom_id":"work_52987093","summary":"Amplification of minute quantities of DNA is a fundamental challenge in low-biomass metagenomic and microbiome studies because of potential biases in coverage, guanine-cytosine (GC) content, and altered species abundances. Whole genome amplification (WGA), although widely used, is notorious for introducing artifact sequences, either by amplifying laboratory contaminants or by nonrandom amplification of a sample's DNA. In this study, we investigate the effect of REPLI-g multiple displacement amplification (MDA; Qiagen, Valencia, CA, USA) on sequencing data quality and species abundance detection in 8 paired metagenomic samples and 1 titrated, mixed control sample. We extracted and sequenced genomic DNA (gDNA) from 8 environmental samples and compared the quality of the sequencing data for the MDA and their corresponding non-MDA samples. The degree of REPLI-g MDA bias was evaluated by sequence metrics, species composition, and crossvalidating observed species abundance and species diversity estimates using the One Codex and MetaPhlAn taxonomic classification tools. Here, we provide evidence of the overall efficacy of REPLI-g MDA on retaining sequencing data quality and species abundance measurements while providing increased yields of high-fidelity DNA. We find that species abundance estimates are largely consistent across samples, even with REPLI-g amplification, as demonstrated by the Spearman's rank order coefficient (R 2. 0.8). However, REPLI-g MDA often produced fewer classified reads at the species, genera, and family level, resulting in decreased species diversity. We also observed some areas with the PCR \"jackpot effect,\" with varying input DNA values for the Metagenomics Research Group (MGRG) controls at specific genomic loci. We visualize this effect in whole genome coverage plots and with sequence composition analyses and note these caveats of the MDA method. Despite overall concordance of species abundance between the amplified and unamplified samples, these results demonstrate that amplification of DNA using the REPLI-g method has some limitations. These concerns could be addressed by future improvements in the enzymes or methods for REPLI-g to be considered a .99% robust method for increasing the amount of high-fidelity DNA from low-biomass samples or at the very least, accounted for during computational analysis of MDA samples.","downloadable_attachments":[{"id":69984220,"asset_id":52987093,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":40860722,"first_name":"Daniela","last_name":"Bezdan","domain_name":"independent","page_name":"DanielaBezdan","display_name":"Daniela Bezdan","profile_url":"https://independent.academia.edu/DanielaBezdan?f_ri=1444","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":923,"name":"Technology","url":"https://www.academia.edu/Documents/in/Technology?f_ri=1444","nofollow":true},{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true},{"id":21948,"name":"Metagenomics","url":"https://www.academia.edu/Documents/in/Metagenomics?f_ri=1444","nofollow":true},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=1444","nofollow":true},{"id":537754,"name":"Microbiota","url":"https://www.academia.edu/Documents/in/Microbiota?f_ri=1444"},{"id":3763225,"name":"Medical and Health Sciences","url":"https://www.academia.edu/Documents/in/Medical_and_Health_Sciences?f_ri=1444"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_10520826" data-work_id="10520826" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/10520826/Interplay_of_metagenomics_and_in_vitro_compartmentalization">Interplay of metagenomics and in vitro compartmentalization</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">In recent years, the application of approaches for harvesting DNA from the environment, the so-called, 'metagenomic approaches' has proven to be highly successful for the identification, isolation and generation of novel enzymes.... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_10520826" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">In recent years, the application of approaches for harvesting DNA from the environment, the so-called, 'metagenomic approaches' has proven to be highly successful for the identification, isolation and generation of novel enzymes. Functional screening for the desired catalytic activity is one of the key steps in mining metagenomic libraries, as it does not rely on sequence homology. In this mini-review, we survey high-throughput screening tools, originally developed for directed evolution experiments, which can be readily adapted for the screening of large libraries. In particular, we focus on the use of in vitro compartmentalization (IVC) approaches to address potential advantages and problems the merger of cultureindependent and IVC techniques might bring on the mining of enzyme activities in microbial communities.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/10520826" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="11c6f21021284151ef9529d0b831b356" rel="nofollow" data-download="{"attachment_id":47330874,"asset_id":10520826,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/47330874/download_file?st=MTc0MDU3NTMzMyw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="25786911" href="https://independent.academia.edu/ManuelGustavoFerrer">Manuel Gustavo Ferrer</a><script data-card-contents-for-user="25786911" type="text/json">{"id":25786911,"first_name":"Manuel Gustavo","last_name":"Ferrer","domain_name":"independent","page_name":"ManuelGustavoFerrer","display_name":"Manuel Gustavo Ferrer","profile_url":"https://independent.academia.edu/ManuelGustavoFerrer?f_ri=1444","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_10520826 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="10520826"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 10520826, container: ".js-paper-rank-work_10520826", }); 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$(".js-view-count[data-work-id=10520826]").text(description); $(".js-view-count-work_10520826").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_10520826").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="10520826"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">5</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="159" rel="nofollow" href="https://www.academia.edu/Documents/in/Microbiology">Microbiology</a>, <script data-card-contents-for-ri="159" type="text/json">{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="1444" rel="nofollow" href="https://www.academia.edu/Documents/in/Environmental_microbiology">Environmental microbiology</a>, <script data-card-contents-for-ri="1444" type="text/json">{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="16645" rel="nofollow" href="https://www.academia.edu/Documents/in/Microbial_biotechnology">Microbial biotechnology</a>, <script data-card-contents-for-ri="16645" type="text/json">{"id":16645,"name":"Microbial biotechnology","url":"https://www.academia.edu/Documents/in/Microbial_biotechnology?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="21948" rel="nofollow" href="https://www.academia.edu/Documents/in/Metagenomics">Metagenomics</a><script data-card-contents-for-ri="21948" type="text/json">{"id":21948,"name":"Metagenomics","url":"https://www.academia.edu/Documents/in/Metagenomics?f_ri=1444","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=10520826]'), work: {"id":10520826,"title":"Interplay of metagenomics and in vitro compartmentalization","created_at":"2015-02-04T15:08:01.050-08:00","url":"https://www.academia.edu/10520826/Interplay_of_metagenomics_and_in_vitro_compartmentalization?f_ri=1444","dom_id":"work_10520826","summary":"In recent years, the application of approaches for harvesting DNA from the environment, the so-called, 'metagenomic approaches' has proven to be highly successful for the identification, isolation and generation of novel enzymes. Functional screening for the desired catalytic activity is one of the key steps in mining metagenomic libraries, as it does not rely on sequence homology. In this mini-review, we survey high-throughput screening tools, originally developed for directed evolution experiments, which can be readily adapted for the screening of large libraries. In particular, we focus on the use of in vitro compartmentalization (IVC) approaches to address potential advantages and problems the merger of cultureindependent and IVC techniques might bring on the mining of enzyme activities in microbial communities.","downloadable_attachments":[{"id":47330874,"asset_id":10520826,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":25786911,"first_name":"Manuel Gustavo","last_name":"Ferrer","domain_name":"independent","page_name":"ManuelGustavoFerrer","display_name":"Manuel Gustavo Ferrer","profile_url":"https://independent.academia.edu/ManuelGustavoFerrer?f_ri=1444","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=1444","nofollow":true},{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true},{"id":16645,"name":"Microbial biotechnology","url":"https://www.academia.edu/Documents/in/Microbial_biotechnology?f_ri=1444","nofollow":true},{"id":21948,"name":"Metagenomics","url":"https://www.academia.edu/Documents/in/Metagenomics?f_ri=1444","nofollow":true},{"id":48579,"name":"Microbial Biotechnology","url":"https://www.academia.edu/Documents/in/Microbial_Biotechnology-1?f_ri=1444"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_26649327" data-work_id="26649327" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" rel="nofollow" href="https://www.academia.edu/26649327/Fluctuations_in_the_occurrence_of_Escherichia_coli_O157_H7_on_a_Norwegian_farm_">Fluctuations in the occurrence of Escherichia coli O157:H7 on a Norwegian farm*</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/26649327" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="5e2e6af820a663b7c077e99b73e3ea59" rel="nofollow" data-download="{"attachment_id":46937005,"asset_id":26649327,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/46937005/download_file?st=MTc0MDU3NTMzMyw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="50623373" rel="nofollow" href="https://independent.academia.edu/AnneUrdahl">Anne Urdahl</a><script data-card-contents-for-user="50623373" type="text/json">{"id":50623373,"first_name":"Anne","last_name":"Urdahl","domain_name":"independent","page_name":"AnneUrdahl","display_name":"Anne Urdahl","profile_url":"https://independent.academia.edu/AnneUrdahl?f_ri=1444","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_26649327 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" 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data-has-card-for-ri="1444" rel="nofollow" href="https://www.academia.edu/Documents/in/Environmental_microbiology">Environmental microbiology</a>, <script data-card-contents-for-ri="1444" type="text/json">{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="10225" rel="nofollow" href="https://www.academia.edu/Documents/in/Agriculture">Agriculture</a>, <script data-card-contents-for-ri="10225" type="text/json">{"id":10225,"name":"Agriculture","url":"https://www.academia.edu/Documents/in/Agriculture?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="28235" rel="nofollow" href="https://www.academia.edu/Documents/in/Multidisciplinary">Multidisciplinary</a>, <script data-card-contents-for-ri="28235" type="text/json">{"id":28235,"name":"Multidisciplinary","url":"https://www.academia.edu/Documents/in/Multidisciplinary?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="51175" rel="nofollow" href="https://www.academia.edu/Documents/in/Organic_Farming">Organic Farming</a><script data-card-contents-for-ri="51175" type="text/json">{"id":51175,"name":"Organic Farming","url":"https://www.academia.edu/Documents/in/Organic_Farming?f_ri=1444","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=26649327]'), work: {"id":26649327,"title":"Fluctuations in the occurrence of Escherichia coli O157:H7 on a Norwegian farm*","created_at":"2016-07-01T00:48:12.684-07:00","url":"https://www.academia.edu/26649327/Fluctuations_in_the_occurrence_of_Escherichia_coli_O157_H7_on_a_Norwegian_farm_?f_ri=1444","dom_id":"work_26649327","summary":null,"downloadable_attachments":[{"id":46937005,"asset_id":26649327,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":50623373,"first_name":"Anne","last_name":"Urdahl","domain_name":"independent","page_name":"AnneUrdahl","display_name":"Anne Urdahl","profile_url":"https://independent.academia.edu/AnneUrdahl?f_ri=1444","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true},{"id":10225,"name":"Agriculture","url":"https://www.academia.edu/Documents/in/Agriculture?f_ri=1444","nofollow":true},{"id":28235,"name":"Multidisciplinary","url":"https://www.academia.edu/Documents/in/Multidisciplinary?f_ri=1444","nofollow":true},{"id":51175,"name":"Organic Farming","url":"https://www.academia.edu/Documents/in/Organic_Farming?f_ri=1444","nofollow":true},{"id":51645,"name":"Norway","url":"https://www.academia.edu/Documents/in/Norway?f_ri=1444"},{"id":52438,"name":"Dogs","url":"https://www.academia.edu/Documents/in/Dogs?f_ri=1444"},{"id":83128,"name":"Escherichia coli","url":"https://www.academia.edu/Documents/in/Escherichia_coli?f_ri=1444"},{"id":84913,"name":"Sheep","url":"https://www.academia.edu/Documents/in/Sheep?f_ri=1444"},{"id":168196,"name":"Horses","url":"https://www.academia.edu/Documents/in/Horses?f_ri=1444"},{"id":260829,"name":"Cattle","url":"https://www.academia.edu/Documents/in/Cattle?f_ri=1444"},{"id":402759,"name":"Chickens","url":"https://www.academia.edu/Documents/in/Chickens?f_ri=1444"},{"id":466867,"name":"Manure","url":"https://www.academia.edu/Documents/in/Manure?f_ri=1444"},{"id":469102,"name":"Feces","url":"https://www.academia.edu/Documents/in/Feces?f_ri=1444"},{"id":515695,"name":"Pulsed field gel electrophoresis (PFGE)","url":"https://www.academia.edu/Documents/in/Pulsed_field_gel_electrophoresis_PFGE_?f_ri=1444"},{"id":809862,"name":"PFGE","url":"https://www.academia.edu/Documents/in/PFGE?f_ri=1444"},{"id":1684568,"name":"Escherichia Coli Infections","url":"https://www.academia.edu/Documents/in/Escherichia_Coli_Infections?f_ri=1444"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_15171994" data-work_id="15171994" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/15171994/Tomato_seed_and_root_exudate_sugars_composition_utilization_by_Pseudomonas_biocontrol_strains_and_role_in_rhizosphere_colonization">Tomato seed and root exudate sugars: composition, utilization by Pseudomonas biocontrol strains and role in rhizosphere colonization</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">The role of tomato seed and root exudate sugars as nutrients for Pseudomonas biocontrol bacteria was studied. To this end, the major exudate sugars of tomato seeds, seedlings and roots were identi®ed and quanti®ed using high-performance... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_15171994" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">The role of tomato seed and root exudate sugars as nutrients for Pseudomonas biocontrol bacteria was studied. To this end, the major exudate sugars of tomato seeds, seedlings and roots were identi®ed and quanti®ed using high-performance liquid chromatographic (HPLC) analysis. Glucose, fructose and maltose were present in all studied growth stages of the plant, but the ratios of these sugars were strongly dependent on the developmental stage. In order to study the putative role of exudate sugar utilization in rhizosphere colonization, two approaches were adopted. First, after co-inoculation on germinated tomato seeds, the root-colonizing ability of the ef®cient root-colonizing P.¯uorescens strain WCS365 in a gnotobiotic quartz sand±plant nutrient solution system was compared with that of other Pseudomonas biocontrol strains. No correlation was observed between the colonizing ability of a strain and its ability to use the major exudate sugars as the only carbon and energy source. Secondly, a Tn5lacZ mutant of P.¯uorescens strain WCS365, strain PCL1083, was isolated, which is impaired in its ability to grow on simple sugars, including those found in exudate. The mutation appeared to reside in zwf, which encodes glucose-6-phosphate dehydrogenase. The mutant grows as well as the parental strain on other media, including tomato root exudate. After inoculation of germinated sterile tomato seeds, the mutant cells reached the same population levels at the root tip as the wild-type strain, both alone and in competition, indicating that the ability to use exudate sugars does not play a major role in tomato root colonization, despite the fact that sugars have often been reported to represent the major exudate carbon source. This conclusion is supported by the observation that the growth of mutant PCL1083 in vitro is inhibited by glucose, a major exudate sugar, at a concentration of 0.001%, which indicates that the glucose concentration in the tomato rhizosphere is very low.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/15171994" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="df918c300f2cee1fde7b7cf9866b9b24" rel="nofollow" data-download="{"attachment_id":43502306,"asset_id":15171994,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/43502306/download_file?st=MTc0MDU3NTMzMyw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="34221591" href="https://independent.academia.edu/BenLugtenberg">Ben Lugtenberg</a><script data-card-contents-for-user="34221591" type="text/json">{"id":34221591,"first_name":"Ben","last_name":"Lugtenberg","domain_name":"independent","page_name":"BenLugtenberg","display_name":"Ben Lugtenberg","profile_url":"https://independent.academia.edu/BenLugtenberg?f_ri=1444","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_15171994 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="15171994"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 15171994, container: ".js-paper-rank-work_15171994", }); 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$(".js-view-count[data-work-id=15171994]").text(description); $(".js-view-count-work_15171994").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_15171994").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="15171994"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">13</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="159" rel="nofollow" href="https://www.academia.edu/Documents/in/Microbiology">Microbiology</a>, <script data-card-contents-for-ri="159" type="text/json">{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="1444" rel="nofollow" href="https://www.academia.edu/Documents/in/Environmental_microbiology">Environmental microbiology</a>, <script data-card-contents-for-ri="1444" type="text/json">{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="112324" rel="nofollow" href="https://www.academia.edu/Documents/in/Pseudomonas">Pseudomonas</a>, <script data-card-contents-for-ri="112324" type="text/json">{"id":112324,"name":"Pseudomonas","url":"https://www.academia.edu/Documents/in/Pseudomonas?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="188240" rel="nofollow" href="https://www.academia.edu/Documents/in/Tomato">Tomato</a><script data-card-contents-for-ri="188240" type="text/json">{"id":188240,"name":"Tomato","url":"https://www.academia.edu/Documents/in/Tomato?f_ri=1444","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=15171994]'), work: {"id":15171994,"title":"Tomato seed and root exudate sugars: composition, utilization by Pseudomonas biocontrol strains and role in rhizosphere colonization","created_at":"2015-08-25T08:07:21.074-07:00","url":"https://www.academia.edu/15171994/Tomato_seed_and_root_exudate_sugars_composition_utilization_by_Pseudomonas_biocontrol_strains_and_role_in_rhizosphere_colonization?f_ri=1444","dom_id":"work_15171994","summary":"The role of tomato seed and root exudate sugars as nutrients for Pseudomonas biocontrol bacteria was studied. To this end, the major exudate sugars of tomato seeds, seedlings and roots were identi®ed and quanti®ed using high-performance liquid chromatographic (HPLC) analysis. Glucose, fructose and maltose were present in all studied growth stages of the plant, but the ratios of these sugars were strongly dependent on the developmental stage. In order to study the putative role of exudate sugar utilization in rhizosphere colonization, two approaches were adopted. First, after co-inoculation on germinated tomato seeds, the root-colonizing ability of the ef®cient root-colonizing P.¯uorescens strain WCS365 in a gnotobiotic quartz sand±plant nutrient solution system was compared with that of other Pseudomonas biocontrol strains. No correlation was observed between the colonizing ability of a strain and its ability to use the major exudate sugars as the only carbon and energy source. Secondly, a Tn5lacZ mutant of P.¯uorescens strain WCS365, strain PCL1083, was isolated, which is impaired in its ability to grow on simple sugars, including those found in exudate. The mutation appeared to reside in zwf, which encodes glucose-6-phosphate dehydrogenase. The mutant grows as well as the parental strain on other media, including tomato root exudate. After inoculation of germinated sterile tomato seeds, the mutant cells reached the same population levels at the root tip as the wild-type strain, both alone and in competition, indicating that the ability to use exudate sugars does not play a major role in tomato root colonization, despite the fact that sugars have often been reported to represent the major exudate carbon source. This conclusion is supported by the observation that the growth of mutant PCL1083 in vitro is inhibited by glucose, a major exudate sugar, at a concentration of 0.001%, which indicates that the glucose concentration in the tomato rhizosphere is very low.","downloadable_attachments":[{"id":43502306,"asset_id":15171994,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":34221591,"first_name":"Ben","last_name":"Lugtenberg","domain_name":"independent","page_name":"BenLugtenberg","display_name":"Ben Lugtenberg","profile_url":"https://independent.academia.edu/BenLugtenberg?f_ri=1444","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=1444","nofollow":true},{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true},{"id":112324,"name":"Pseudomonas","url":"https://www.academia.edu/Documents/in/Pseudomonas?f_ri=1444","nofollow":true},{"id":188240,"name":"Tomato","url":"https://www.academia.edu/Documents/in/Tomato?f_ri=1444","nofollow":true},{"id":202399,"name":"Plant Roots","url":"https://www.academia.edu/Documents/in/Plant_Roots?f_ri=1444"},{"id":231547,"name":"Soil Microbiology","url":"https://www.academia.edu/Documents/in/Soil_Microbiology?f_ri=1444"},{"id":246560,"name":"High Pressure Liquid Chromatography","url":"https://www.academia.edu/Documents/in/High_Pressure_Liquid_Chromatography?f_ri=1444"},{"id":386438,"name":"Seeds","url":"https://www.academia.edu/Documents/in/Seeds?f_ri=1444"},{"id":409525,"name":"Carbohydrate metabolism","url":"https://www.academia.edu/Documents/in/Carbohydrate_metabolism?f_ri=1444"},{"id":609249,"name":"CARBOHYDRATES","url":"https://www.academia.edu/Documents/in/CARBOHYDRATES?f_ri=1444"},{"id":957359,"name":"Culture Media","url":"https://www.academia.edu/Documents/in/Culture_Media?f_ri=1444"},{"id":1133132,"name":"Environmental","url":"https://www.academia.edu/Documents/in/Environmental?f_ri=1444"},{"id":1640340,"name":"Lycopersicon esculentum","url":"https://www.academia.edu/Documents/in/Lycopersicon_esculentum?f_ri=1444"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_15691404" data-work_id="15691404" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/15691404/A_first_approximation_kinetic_model_to_predict_methane_generation_from_an_oil_sands_tailings_settling_basin">A first approximation kinetic model to predict methane generation from an oil sands tailings settling basin</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">A small fraction of the naphtha diluent used for oil sands processing escapes with tailings and supports methane (CH 4 ) biogenesis in large anaerobic settling basins such as Mildred Lake Settling Basin (MLSB) in northern Alberta, Canada.... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_15691404" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">A small fraction of the naphtha diluent used for oil sands processing escapes with tailings and supports methane (CH 4 ) biogenesis in large anaerobic settling basins such as Mildred Lake Settling Basin (MLSB) in northern Alberta, Canada. Based on the rate of naphtha metabolism in tailings incubated in laboratory microcosms, a kinetic model comprising lag phase, rate of hydrocarbon metabolism and conversion to CH 4 was developed to predict CH 4 biogenesis and flux from MLSB. Zero-and first-order kinetic models, respectively predicted generation of 5.4 and 5.1 mmol CH 4 in naphtha-amended microcosms compared to 5.3 (±0.2) mmol CH 4 measured in microcosms during 46 weeks of incubation. These kinetic models also predicted well the CH 4 produced by tailings amended with either naphtha-range n-alkanes or BTEX compounds at concentrations similar to those expected in MLSB. Considering 25% of MLSB's 200 million m 3 tailings volume to be methanogenic, the zero-and first-order kinetic models applied over a wide range of naphtha concentrations (0.01-1.0 wt%) predicted production of 8.9-400 million l CH 4 day À1 from MLSB, which exceeds the estimated production of 3-43 million l CH 4 day À1 . This discrepancy may result from heterogeneity and density of the tailings, presence of nutrients in the microcosms, and/or overestimation of the readily biodegradable fraction of the naphtha in MLSB tailings.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/15691404" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="cb4f9e80b0899b7ab0b2a0c7873853d9" rel="nofollow" data-download="{"attachment_id":42957228,"asset_id":15691404,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/42957228/download_file?st=MTc0MDU3NTMzMyw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="34879848" href="https://independent.academia.edu/PhillipFedorak">Phillip Fedorak</a><script data-card-contents-for-user="34879848" type="text/json">{"id":34879848,"first_name":"Phillip","last_name":"Fedorak","domain_name":"independent","page_name":"PhillipFedorak","display_name":"Phillip Fedorak","profile_url":"https://independent.academia.edu/PhillipFedorak?f_ri=1444","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_15691404 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="15691404"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 15691404, container: ".js-paper-rank-work_15691404", }); 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$(".js-view-count[data-work-id=15691404]").text(description); $(".js-view-count-work_15691404").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_15691404").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="15691404"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">14</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="1444" rel="nofollow" href="https://www.academia.edu/Documents/in/Environmental_microbiology">Environmental microbiology</a>, <script data-card-contents-for-ri="1444" type="text/json">{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="4987" rel="nofollow" href="https://www.academia.edu/Documents/in/Kinetics">Kinetics</a>, <script data-card-contents-for-ri="4987" type="text/json">{"id":4987,"name":"Kinetics","url":"https://www.academia.edu/Documents/in/Kinetics?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="5285" rel="nofollow" href="https://www.academia.edu/Documents/in/Petroleum">Petroleum</a>, <script data-card-contents-for-ri="5285" type="text/json">{"id":5285,"name":"Petroleum","url":"https://www.academia.edu/Documents/in/Petroleum?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="28235" rel="nofollow" href="https://www.academia.edu/Documents/in/Multidisciplinary">Multidisciplinary</a><script data-card-contents-for-ri="28235" type="text/json">{"id":28235,"name":"Multidisciplinary","url":"https://www.academia.edu/Documents/in/Multidisciplinary?f_ri=1444","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=15691404]'), work: {"id":15691404,"title":"A first approximation kinetic model to predict methane generation from an oil sands tailings settling basin","created_at":"2015-09-14T11:11:56.038-07:00","url":"https://www.academia.edu/15691404/A_first_approximation_kinetic_model_to_predict_methane_generation_from_an_oil_sands_tailings_settling_basin?f_ri=1444","dom_id":"work_15691404","summary":"A small fraction of the naphtha diluent used for oil sands processing escapes with tailings and supports methane (CH 4 ) biogenesis in large anaerobic settling basins such as Mildred Lake Settling Basin (MLSB) in northern Alberta, Canada. Based on the rate of naphtha metabolism in tailings incubated in laboratory microcosms, a kinetic model comprising lag phase, rate of hydrocarbon metabolism and conversion to CH 4 was developed to predict CH 4 biogenesis and flux from MLSB. Zero-and first-order kinetic models, respectively predicted generation of 5.4 and 5.1 mmol CH 4 in naphtha-amended microcosms compared to 5.3 (±0.2) mmol CH 4 measured in microcosms during 46 weeks of incubation. These kinetic models also predicted well the CH 4 produced by tailings amended with either naphtha-range n-alkanes or BTEX compounds at concentrations similar to those expected in MLSB. Considering 25% of MLSB's 200 million m 3 tailings volume to be methanogenic, the zero-and first-order kinetic models applied over a wide range of naphtha concentrations (0.01-1.0 wt%) predicted production of 8.9-400 million l CH 4 day À1 from MLSB, which exceeds the estimated production of 3-43 million l CH 4 day À1 . This discrepancy may result from heterogeneity and density of the tailings, presence of nutrients in the microcosms, and/or overestimation of the readily biodegradable fraction of the naphtha in MLSB tailings.","downloadable_attachments":[{"id":42957228,"asset_id":15691404,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":34879848,"first_name":"Phillip","last_name":"Fedorak","domain_name":"independent","page_name":"PhillipFedorak","display_name":"Phillip Fedorak","profile_url":"https://independent.academia.edu/PhillipFedorak?f_ri=1444","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true},{"id":4987,"name":"Kinetics","url":"https://www.academia.edu/Documents/in/Kinetics?f_ri=1444","nofollow":true},{"id":5285,"name":"Petroleum","url":"https://www.academia.edu/Documents/in/Petroleum?f_ri=1444","nofollow":true},{"id":28235,"name":"Multidisciplinary","url":"https://www.academia.edu/Documents/in/Multidisciplinary?f_ri=1444","nofollow":true},{"id":49088,"name":"Biodegradation","url":"https://www.academia.edu/Documents/in/Biodegradation?f_ri=1444"},{"id":156347,"name":"Methane","url":"https://www.academia.edu/Documents/in/Methane?f_ri=1444"},{"id":181847,"name":"First-Order Logic","url":"https://www.academia.edu/Documents/in/First-Order_Logic?f_ri=1444"},{"id":251654,"name":"Greenhouse Gas","url":"https://www.academia.edu/Documents/in/Greenhouse_Gas?f_ri=1444"},{"id":343667,"name":"Theoretical Models","url":"https://www.academia.edu/Documents/in/Theoretical_Models?f_ri=1444"},{"id":356669,"name":"Alberta","url":"https://www.academia.edu/Documents/in/Alberta?f_ri=1444"},{"id":397099,"name":"Biological treatment","url":"https://www.academia.edu/Documents/in/Biological_treatment?f_ri=1444"},{"id":410194,"name":"Waste Treatment","url":"https://www.academia.edu/Documents/in/Waste_Treatment?f_ri=1444"},{"id":835697,"name":"Lumping Kinetic Model","url":"https://www.academia.edu/Documents/in/Lumping_Kinetic_Model?f_ri=1444"},{"id":1145788,"name":"Alkanes","url":"https://www.academia.edu/Documents/in/Alkanes?f_ri=1444"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_20401334" data-work_id="20401334" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/20401334/Investigation_of_risk_factors_for_Salmonella_on_commercial_egg_laying_farms_in_Great_Britain_2004_2005">Investigation of risk factors for Salmonella on commercial egg-laying farms in Great Britain, 2004-2005</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">In 2004/05, all European Union member states were required to carry out standardised prevalence surveys to establish the baseline prevalence of Salmonella in commercial laying flocks. As part of the survey in Great Britain, additional... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_20401334" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">In 2004/05, all European Union member states were required to carry out standardised prevalence surveys to establish the baseline prevalence of Salmonella in commercial laying flocks. As part of the survey in Great Britain, additional data were collected from 380 of the enrolled laying hen holdings to investigate risk factors for Salmonella at farm level. Stratified, simple random sampling was used to select holdings from which dust and boot swab samples were collected and tested for Salmonella using a modification of ISO 6579:2002. Using a multivariable logistic model weighted to account for the survey design, several factors significantly associated with Salmonella and Salmonella Enteritidis status were identified. Larger holdings (≥30,000 birds) were found to be at higher risk of Salmonella odds ratio [OR] 4.79, P=0.025), while vaccination (OR 0.28, P=0.013), providing foot dips with brushes (OR 0.27, P=0.042), washing and disinfecting the house at depopulation (OR 0.19, P=0.003), having a clean car park away from house (OR 0.14, P=0.001), using an independent (OR 0.19, P=0.007) or other non-company (OR 0.40, P=0.049) source of feed, being over 1 km from the nearest neighbouring farm (OR 0.45, P=0.021) and the presence of cats and dogs on the farm (OR 0.26, P=0.002) or on contiguous farms (OR 0.44, P=0.030) reduced the risk of any Salmonella serovars being present. Factors found to be associated specifically with an increased risk of S Enteritidis infection included holding size (OR 14.88, P=0.001) and frequent sightings of rats (OR 8.17, P<0.001) or mice (OR 5.78, P=0.006). Non-caged systems (OR 0.14, P=0.002), vaccination (OR 0.08, P=0.001), the use of a non-company feed source (OR 0.11, P=0.003), running the site as all-in/all-out (OR 0.06, P<0.001) and the presence of cats and dogs on the farm (OR 0.14, P=0.002) were associated with a reduced risk.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/20401334" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="6ab16c01c20722ffda1ec483cfe65b39" rel="nofollow" data-download="{"attachment_id":41343439,"asset_id":20401334,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/41343439/download_file?st=MTc0MDU3NTMzMyw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="41783758" href="https://surrery.academia.edu/SuziAlexCook">Alasdair J C Cook</a><script data-card-contents-for-user="41783758" type="text/json">{"id":41783758,"first_name":"Alasdair","last_name":"Cook","domain_name":"surrery","page_name":"SuziAlexCook","display_name":"Alasdair J C Cook","profile_url":"https://surrery.academia.edu/SuziAlexCook?f_ri=1444","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_20401334 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="20401334"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 20401334, container: ".js-paper-rank-work_20401334", }); });</script></li><li class="js-percentile-work_20401334 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 20401334; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_20401334"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_20401334 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="20401334"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 20401334; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=20401334]").text(description); $(".js-view-count-work_20401334").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_20401334").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="20401334"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">16</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="1444" rel="nofollow" href="https://www.academia.edu/Documents/in/Environmental_microbiology">Environmental microbiology</a>, <script data-card-contents-for-ri="1444" type="text/json">{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="35414" rel="nofollow" href="https://www.academia.edu/Documents/in/Veterinary">Veterinary</a>, <script data-card-contents-for-ri="35414" type="text/json">{"id":35414,"name":"Veterinary","url":"https://www.academia.edu/Documents/in/Veterinary?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="37881" rel="nofollow" href="https://www.academia.edu/Documents/in/Animal_Husbandry">Animal Husbandry</a>, <script data-card-contents-for-ri="37881" type="text/json">{"id":37881,"name":"Animal Husbandry","url":"https://www.academia.edu/Documents/in/Animal_Husbandry?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="162645" rel="nofollow" href="https://www.academia.edu/Documents/in/Population_Density">Population Density</a><script data-card-contents-for-ri="162645" type="text/json">{"id":162645,"name":"Population Density","url":"https://www.academia.edu/Documents/in/Population_Density?f_ri=1444","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=20401334]'), work: {"id":20401334,"title":"Investigation of risk factors for Salmonella on commercial egg-laying farms in Great Britain, 2004-2005","created_at":"2016-01-20T01:09:01.004-08:00","url":"https://www.academia.edu/20401334/Investigation_of_risk_factors_for_Salmonella_on_commercial_egg_laying_farms_in_Great_Britain_2004_2005?f_ri=1444","dom_id":"work_20401334","summary":"In 2004/05, all European Union member states were required to carry out standardised prevalence surveys to establish the baseline prevalence of Salmonella in commercial laying flocks. As part of the survey in Great Britain, additional data were collected from 380 of the enrolled laying hen holdings to investigate risk factors for Salmonella at farm level. Stratified, simple random sampling was used to select holdings from which dust and boot swab samples were collected and tested for Salmonella using a modification of ISO 6579:2002. Using a multivariable logistic model weighted to account for the survey design, several factors significantly associated with Salmonella and Salmonella Enteritidis status were identified. Larger holdings (≥30,000 birds) were found to be at higher risk of Salmonella odds ratio [OR] 4.79, P=0.025), while vaccination (OR 0.28, P=0.013), providing foot dips with brushes (OR 0.27, P=0.042), washing and disinfecting the house at depopulation (OR 0.19, P=0.003), having a clean car park away from house (OR 0.14, P=0.001), using an independent (OR 0.19, P=0.007) or other non-company (OR 0.40, P=0.049) source of feed, being over 1 km from the nearest neighbouring farm (OR 0.45, P=0.021) and the presence of cats and dogs on the farm (OR 0.26, P=0.002) or on contiguous farms (OR 0.44, P=0.030) reduced the risk of any Salmonella serovars being present. Factors found to be associated specifically with an increased risk of S Enteritidis infection included holding size (OR 14.88, P=0.001) and frequent sightings of rats (OR 8.17, P\u003c0.001) or mice (OR 5.78, P=0.006). Non-caged systems (OR 0.14, P=0.002), vaccination (OR 0.08, P=0.001), the use of a non-company feed source (OR 0.11, P=0.003), running the site as all-in/all-out (OR 0.06, P\u003c0.001) and the presence of cats and dogs on the farm (OR 0.14, P=0.002) were associated with a reduced risk.","downloadable_attachments":[{"id":41343439,"asset_id":20401334,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":41783758,"first_name":"Alasdair","last_name":"Cook","domain_name":"surrery","page_name":"SuziAlexCook","display_name":"Alasdair J C Cook","profile_url":"https://surrery.academia.edu/SuziAlexCook?f_ri=1444","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true},{"id":35414,"name":"Veterinary","url":"https://www.academia.edu/Documents/in/Veterinary?f_ri=1444","nofollow":true},{"id":37881,"name":"Animal Husbandry","url":"https://www.academia.edu/Documents/in/Animal_Husbandry?f_ri=1444","nofollow":true},{"id":162645,"name":"Population Density","url":"https://www.academia.edu/Documents/in/Population_Density?f_ri=1444","nofollow":true},{"id":192721,"name":"Risk factors","url":"https://www.academia.edu/Documents/in/Risk_factors?f_ri=1444"},{"id":257897,"name":"Salmonella","url":"https://www.academia.edu/Documents/in/Salmonella?f_ri=1444"},{"id":289330,"name":"Prevalence","url":"https://www.academia.edu/Documents/in/Prevalence?f_ri=1444"},{"id":291274,"name":"Great Britain","url":"https://www.academia.edu/Documents/in/Great_Britain?f_ri=1444"},{"id":336260,"name":"Eggs","url":"https://www.academia.edu/Documents/in/Eggs?f_ri=1444"},{"id":366620,"name":"POULTRY DISEASES","url":"https://www.academia.edu/Documents/in/POULTRY_DISEASES?f_ri=1444"},{"id":402759,"name":"Chickens","url":"https://www.academia.edu/Documents/in/Chickens?f_ri=1444"},{"id":437772,"name":"Odds ratio","url":"https://www.academia.edu/Documents/in/Odds_ratio?f_ri=1444"},{"id":570860,"name":"Food Contamination","url":"https://www.academia.edu/Documents/in/Food_Contamination?f_ri=1444"},{"id":620049,"name":"Risk Factors","url":"https://www.academia.edu/Documents/in/Risk_Factors-1?f_ri=1444"},{"id":644860,"name":"Veterinary Sciences","url":"https://www.academia.edu/Documents/in/Veterinary_Sciences?f_ri=1444"},{"id":1294607,"name":"Logistic Models","url":"https://www.academia.edu/Documents/in/Logistic_Models?f_ri=1444"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_18630819" data-work_id="18630819" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/18630819/Denitrifying_bacteria_anaerobically_oxidize_methane_in_the_absence_of_Archaea">Denitrifying bacteria anaerobically oxidize methane in the absence of Archaea</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Recently, a microbial consortium was shown to couple the anaerobic oxidation of methane to denitrification, predominantly in the form of nitrite reduction to dinitrogen gas. This consortium was dominated by bacteria of an as yet... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_18630819" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Recently, a microbial consortium was shown to couple the anaerobic oxidation of methane to denitrification, predominantly in the form of nitrite reduction to dinitrogen gas. This consortium was dominated by bacteria of an as yet uncharacterized division and archaea of the order Methanosarcinales. The present manuscript reports on the upscaling of the enrichment culture, and addresses the role of the archaea in methane oxidation. The key gene of methanotrophic and methanogenic archaea, mcrA, was sequenced. The associated cofactor F 430 was shown to have a mass of 905 Da, the same as for methanogens and different from the heavier form (951 Da) found in methanotrophic archaea. After prolonged enrichment (> 1 year), no inhibition of anaerobic methane oxidation was observed in the presence of 20 mM bromoethane sulfonate, a specific inhibitor of MCR. Optimization of the cultivation conditions led to higher rates of methane oxidation and to the decline of the archaeal population, as shown by fluorescence in situ hybridization and quantitative MALDI-TOF analysis of F 430. Mass balancing showed that methane oxidation was still coupled to nitrite reduction in the total absence of oxygen. Together, our results show that bacteria can couple the anaerobic oxidation of methane to denitrification without the involvement of Archaea. Fig. 1. Continuous culture set-up with biomass retention in an external settler.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/18630819" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="24d79d2dbe7c4879ee3a4c2fdb41cd18" rel="nofollow" data-download="{"attachment_id":40168598,"asset_id":18630819,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/40168598/download_file?st=MTc0MDU3NTMzMyw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="38646890" href="https://independent.academia.edu/MarnixMedema">Marnix Medema</a><script data-card-contents-for-user="38646890" type="text/json">{"id":38646890,"first_name":"Marnix","last_name":"Medema","domain_name":"independent","page_name":"MarnixMedema","display_name":"Marnix Medema","profile_url":"https://independent.academia.edu/MarnixMedema?f_ri=1444","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_18630819 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="18630819"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 18630819, container: ".js-paper-rank-work_18630819", }); });</script></li><li class="js-percentile-work_18630819 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 18630819; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_18630819"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_18630819 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="18630819"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 18630819; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=18630819]").text(description); $(".js-view-count-work_18630819").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_18630819").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="18630819"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">7</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="159" rel="nofollow" href="https://www.academia.edu/Documents/in/Microbiology">Microbiology</a>, <script data-card-contents-for-ri="159" type="text/json">{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="1444" rel="nofollow" href="https://www.academia.edu/Documents/in/Environmental_microbiology">Environmental microbiology</a>, <script data-card-contents-for-ri="1444" type="text/json">{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="104196" rel="nofollow" href="https://www.academia.edu/Documents/in/Archaea">Archaea</a>, <script data-card-contents-for-ri="104196" type="text/json">{"id":104196,"name":"Archaea","url":"https://www.academia.edu/Documents/in/Archaea?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="151091" rel="nofollow" href="https://www.academia.edu/Documents/in/Nitrogen">Nitrogen</a><script data-card-contents-for-ri="151091" type="text/json">{"id":151091,"name":"Nitrogen","url":"https://www.academia.edu/Documents/in/Nitrogen?f_ri=1444","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=18630819]'), work: {"id":18630819,"title":"Denitrifying bacteria anaerobically oxidize methane in the absence of Archaea","created_at":"2015-11-19T01:51:21.959-08:00","url":"https://www.academia.edu/18630819/Denitrifying_bacteria_anaerobically_oxidize_methane_in_the_absence_of_Archaea?f_ri=1444","dom_id":"work_18630819","summary":"Recently, a microbial consortium was shown to couple the anaerobic oxidation of methane to denitrification, predominantly in the form of nitrite reduction to dinitrogen gas. This consortium was dominated by bacteria of an as yet uncharacterized division and archaea of the order Methanosarcinales. The present manuscript reports on the upscaling of the enrichment culture, and addresses the role of the archaea in methane oxidation. The key gene of methanotrophic and methanogenic archaea, mcrA, was sequenced. The associated cofactor F 430 was shown to have a mass of 905 Da, the same as for methanogens and different from the heavier form (951 Da) found in methanotrophic archaea. After prolonged enrichment (\u003e 1 year), no inhibition of anaerobic methane oxidation was observed in the presence of 20 mM bromoethane sulfonate, a specific inhibitor of MCR. Optimization of the cultivation conditions led to higher rates of methane oxidation and to the decline of the archaeal population, as shown by fluorescence in situ hybridization and quantitative MALDI-TOF analysis of F 430. Mass balancing showed that methane oxidation was still coupled to nitrite reduction in the total absence of oxygen. Together, our results show that bacteria can couple the anaerobic oxidation of methane to denitrification without the involvement of Archaea. Fig. 1. Continuous culture set-up with biomass retention in an external settler.","downloadable_attachments":[{"id":40168598,"asset_id":18630819,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":38646890,"first_name":"Marnix","last_name":"Medema","domain_name":"independent","page_name":"MarnixMedema","display_name":"Marnix Medema","profile_url":"https://independent.academia.edu/MarnixMedema?f_ri=1444","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=1444","nofollow":true},{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true},{"id":104196,"name":"Archaea","url":"https://www.academia.edu/Documents/in/Archaea?f_ri=1444","nofollow":true},{"id":151091,"name":"Nitrogen","url":"https://www.academia.edu/Documents/in/Nitrogen?f_ri=1444","nofollow":true},{"id":156347,"name":"Methane","url":"https://www.academia.edu/Documents/in/Methane?f_ri=1444"},{"id":1256747,"name":"Oxidation-Reduction","url":"https://www.academia.edu/Documents/in/Oxidation-Reduction?f_ri=1444"},{"id":1654024,"name":"Nitrites","url":"https://www.academia.edu/Documents/in/Nitrites?f_ri=1444"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_77800512" data-work_id="77800512" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/77800512/Phylodynamic_Analysis_of_Clinical_and_Environmental_Vibrio_cholerae_Isolates_from_Haiti_Reveals_Diversification_Driven_by_Positive_Selection">Phylodynamic Analysis of Clinical and Environmental Vibrio cholerae Isolates from Haiti Reveals Diversification Driven by Positive Selection</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Phylodynamic analysis of genome-wide single-nucleotide polymorphism (SNP) data is a powerful tool to investigate underlying evolutionary processes of bacterial epidemics. The method was applied to investigate a collection of 65 clinical... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_77800512" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Phylodynamic analysis of genome-wide single-nucleotide polymorphism (SNP) data is a powerful tool to investigate underlying evolutionary processes of bacterial epidemics. The method was applied to investigate a collection of 65 clinical and environmental isolates of Vibrio cholerae from Haiti collected between 2010 and 2012. Characterization of isolates recovered from environmental samples identified a total of four toxigenic V. cholerae O1 isolates, four non-O1/O139 isolates, and a novel nontoxigenic V. cholerae O1 isolate with the classical tcpA gene. Phylogenies of strains were inferred from genome-wide SNPs using coalescent-based demographic models within a Bayesian framework. A close phylogenetic relationship between clinical and environmental toxigenic V. cholerae O1 strains was observed. As cholera spread throughout Haiti between October 2010 and August 2012, the population size initially increased and then fluctuated over time. Selection analysis along internal branches of t...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/77800512" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="8f2c26c9de6fd7a6a90f369d10821f75" rel="nofollow" data-download="{"attachment_id":85067384,"asset_id":77800512,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/85067384/download_file?st=MTc0MDU3NTMzMyw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="67461644" href="https://independent.academia.edu/MeerAlam3">Meer Alam</a><script data-card-contents-for-user="67461644" type="text/json">{"id":67461644,"first_name":"Meer","last_name":"Alam","domain_name":"independent","page_name":"MeerAlam3","display_name":"Meer Alam","profile_url":"https://independent.academia.edu/MeerAlam3?f_ri=1444","photo":"https://0.academia-photos.com/67461644/30199848/28016152/s65_meer.alam.jpg"}</script></span></span></li><li class="js-paper-rank-work_77800512 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="77800512"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 77800512, container: ".js-paper-rank-work_77800512", }); });</script></li><li class="js-percentile-work_77800512 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 77800512; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_77800512"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_77800512 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="77800512"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 77800512; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=77800512]").text(description); $(".js-view-count-work_77800512").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_77800512").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="77800512"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">8</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="159" rel="nofollow" href="https://www.academia.edu/Documents/in/Microbiology">Microbiology</a>, <script data-card-contents-for-ri="159" type="text/json">{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="1444" rel="nofollow" href="https://www.academia.edu/Documents/in/Environmental_microbiology">Environmental microbiology</a>, <script data-card-contents-for-ri="1444" type="text/json">{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="4967" rel="nofollow" href="https://www.academia.edu/Documents/in/Molecular_Evolution">Molecular Evolution</a>, <script data-card-contents-for-ri="4967" type="text/json">{"id":4967,"name":"Molecular Evolution","url":"https://www.academia.edu/Documents/in/Molecular_Evolution?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="6726" rel="nofollow" href="https://www.academia.edu/Documents/in/Haiti">Haiti</a><script data-card-contents-for-ri="6726" type="text/json">{"id":6726,"name":"Haiti","url":"https://www.academia.edu/Documents/in/Haiti?f_ri=1444","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=77800512]'), work: {"id":77800512,"title":"Phylodynamic Analysis of Clinical and Environmental Vibrio cholerae Isolates from Haiti Reveals Diversification Driven by Positive Selection","created_at":"2022-04-27T10:36:27.626-07:00","url":"https://www.academia.edu/77800512/Phylodynamic_Analysis_of_Clinical_and_Environmental_Vibrio_cholerae_Isolates_from_Haiti_Reveals_Diversification_Driven_by_Positive_Selection?f_ri=1444","dom_id":"work_77800512","summary":"Phylodynamic analysis of genome-wide single-nucleotide polymorphism (SNP) data is a powerful tool to investigate underlying evolutionary processes of bacterial epidemics. The method was applied to investigate a collection of 65 clinical and environmental isolates of Vibrio cholerae from Haiti collected between 2010 and 2012. Characterization of isolates recovered from environmental samples identified a total of four toxigenic V. cholerae O1 isolates, four non-O1/O139 isolates, and a novel nontoxigenic V. cholerae O1 isolate with the classical tcpA gene. Phylogenies of strains were inferred from genome-wide SNPs using coalescent-based demographic models within a Bayesian framework. A close phylogenetic relationship between clinical and environmental toxigenic V. cholerae O1 strains was observed. As cholera spread throughout Haiti between October 2010 and August 2012, the population size initially increased and then fluctuated over time. Selection analysis along internal branches of t...","downloadable_attachments":[{"id":85067384,"asset_id":77800512,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":67461644,"first_name":"Meer","last_name":"Alam","domain_name":"independent","page_name":"MeerAlam3","display_name":"Meer Alam","profile_url":"https://independent.academia.edu/MeerAlam3?f_ri=1444","photo":"https://0.academia-photos.com/67461644/30199848/28016152/s65_meer.alam.jpg"}],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=1444","nofollow":true},{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true},{"id":4967,"name":"Molecular Evolution","url":"https://www.academia.edu/Documents/in/Molecular_Evolution?f_ri=1444","nofollow":true},{"id":6726,"name":"Haiti","url":"https://www.academia.edu/Documents/in/Haiti?f_ri=1444","nofollow":true},{"id":207823,"name":"Cholera","url":"https://www.academia.edu/Documents/in/Cholera?f_ri=1444"},{"id":403740,"name":"Single Nucleotide Polymorphism","url":"https://www.academia.edu/Documents/in/Single_Nucleotide_Polymorphism?f_ri=1444"},{"id":577933,"name":"Genetic variation","url":"https://www.academia.edu/Documents/in/Genetic_variation?f_ri=1444"},{"id":874509,"name":"Mbio","url":"https://www.academia.edu/Documents/in/Mbio?f_ri=1444"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_72219059" data-work_id="72219059" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" rel="nofollow" href="https://www.academia.edu/72219059/Effects_but_no_interactions_of_ubiquitous_pesticide_and_parasite_stressors_on_honey_bee_Apis_mellifera_lifespan_and_behaviour_in_a_colony_environment">Effects, but no interactions, of ubiquitous pesticide and parasite stressors on honey bee (Apis mellifera) lifespan and behaviour in a colony environment</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Interactions between pesticides and parasites are believed to be responsible for increased mortality of honey bee (Apis mellifera) colonies in the northern hemisphere. Previous efforts have employed experimental approaches using small... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_72219059" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Interactions between pesticides and parasites are believed to be responsible for increased mortality of honey bee (Apis mellifera) colonies in the northern hemisphere. Previous efforts have employed experimental approaches using small groups under laboratory conditions to investigate influence of these stressors on honey bee physiology and behaviour, although both the colony level and field conditions play a key role for eusocial honey bees. Here, we challenged honey bee workers under in vivo colony conditions with sublethal doses of the neonicotinoid thiacloprid, the miticide tau-fluvalinate and the endoparasite Nosema ceranae, to investigate potential effects on longevity and behaviour using observation hives. In contrast to previous laboratory studies, our results do not suggest interactions among stressors, but rather lone effects of pesticides and the parasite on mortality and behaviour, respectively. These effects appear to be weak due to different outcomes at the two study si...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/72219059" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="117578731" href="https://independent.academia.edu/PiusWiniger">Pius Winiger</a><script data-card-contents-for-user="117578731" type="text/json">{"id":117578731,"first_name":"Pius","last_name":"Winiger","domain_name":"independent","page_name":"PiusWiniger","display_name":"Pius Winiger","profile_url":"https://independent.academia.edu/PiusWiniger?f_ri=1444","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_72219059 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="72219059"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 72219059, container: ".js-paper-rank-work_72219059", }); });</script></li><li class="js-percentile-work_72219059 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 72219059; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_72219059"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_72219059 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="72219059"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 72219059; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=72219059]").text(description); $(".js-view-count-work_72219059").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_72219059").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="72219059"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">16</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="159" rel="nofollow" href="https://www.academia.edu/Documents/in/Microbiology">Microbiology</a>, <script data-card-contents-for-ri="159" type="text/json">{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="1444" rel="nofollow" href="https://www.academia.edu/Documents/in/Environmental_microbiology">Environmental microbiology</a>, <script data-card-contents-for-ri="1444" type="text/json">{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="4552" rel="nofollow" href="https://www.academia.edu/Documents/in/Ecotoxicology">Ecotoxicology</a>, <script data-card-contents-for-ri="4552" type="text/json">{"id":4552,"name":"Ecotoxicology","url":"https://www.academia.edu/Documents/in/Ecotoxicology?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="7710" rel="nofollow" href="https://www.academia.edu/Documents/in/Biology">Biology</a><script data-card-contents-for-ri="7710" type="text/json">{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology?f_ri=1444","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=72219059]'), work: {"id":72219059,"title":"Effects, but no interactions, of ubiquitous pesticide and parasite stressors on honey bee (Apis mellifera) lifespan and behaviour in a colony environment","created_at":"2022-02-22T05:09:01.910-08:00","url":"https://www.academia.edu/72219059/Effects_but_no_interactions_of_ubiquitous_pesticide_and_parasite_stressors_on_honey_bee_Apis_mellifera_lifespan_and_behaviour_in_a_colony_environment?f_ri=1444","dom_id":"work_72219059","summary":"Interactions between pesticides and parasites are believed to be responsible for increased mortality of honey bee (Apis mellifera) colonies in the northern hemisphere. Previous efforts have employed experimental approaches using small groups under laboratory conditions to investigate influence of these stressors on honey bee physiology and behaviour, although both the colony level and field conditions play a key role for eusocial honey bees. Here, we challenged honey bee workers under in vivo colony conditions with sublethal doses of the neonicotinoid thiacloprid, the miticide tau-fluvalinate and the endoparasite Nosema ceranae, to investigate potential effects on longevity and behaviour using observation hives. In contrast to previous laboratory studies, our results do not suggest interactions among stressors, but rather lone effects of pesticides and the parasite on mortality and behaviour, respectively. These effects appear to be weak due to different outcomes at the two study si...","downloadable_attachments":[],"ordered_authors":[{"id":117578731,"first_name":"Pius","last_name":"Winiger","domain_name":"independent","page_name":"PiusWiniger","display_name":"Pius Winiger","profile_url":"https://independent.academia.edu/PiusWiniger?f_ri=1444","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=1444","nofollow":true},{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true},{"id":4552,"name":"Ecotoxicology","url":"https://www.academia.edu/Documents/in/Ecotoxicology?f_ri=1444","nofollow":true},{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology?f_ri=1444","nofollow":true},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine?f_ri=1444"},{"id":52972,"name":"Insecticides","url":"https://www.academia.edu/Documents/in/Insecticides?f_ri=1444"},{"id":59690,"name":"Bees","url":"https://www.academia.edu/Documents/in/Bees?f_ri=1444"},{"id":64753,"name":"Apis mellifera","url":"https://www.academia.edu/Documents/in/Apis_mellifera?f_ri=1444"},{"id":83906,"name":"Nitriles","url":"https://www.academia.edu/Documents/in/Nitriles?f_ri=1444"},{"id":265148,"name":"Honey Bees","url":"https://www.academia.edu/Documents/in/Honey_Bees?f_ri=1444"},{"id":327811,"name":"Nosema","url":"https://www.academia.edu/Documents/in/Nosema?f_ri=1444"},{"id":490260,"name":"Pyridines","url":"https://www.academia.edu/Documents/in/Pyridines?f_ri=1444"},{"id":1349227,"name":"Thiazines","url":"https://www.academia.edu/Documents/in/Thiazines?f_ri=1444"},{"id":2491097,"name":"Nosema ceranae","url":"https://www.academia.edu/Documents/in/Nosema_ceranae?f_ri=1444"},{"id":2872479,"name":"Neonicotinoids","url":"https://www.academia.edu/Documents/in/Neonicotinoids?f_ri=1444"},{"id":2913845,"name":"Thiacloprid","url":"https://www.academia.edu/Documents/in/Thiacloprid?f_ri=1444"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_3790390" data-work_id="3790390" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/3790390/The_marine_fireworm_Hermodice_carunculata_is_a_winter_reservoir_and_spring_summer_vector_for_the_coral_bleaching_pathogen_Vibrio_shiloi">The marine fireworm Hermodice carunculata is a winter reservoir and spring-summer vector for the coral-bleaching pathogen Vibrio shiloi</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Vibrio shiloi , the causative agent of bleaching of the coral Oculina patagonica in the Mediterranean Sea, is present in all bleached O. patagonica corals in the summer (25-30 ∞ ∞ ∞ ∞ C), but can be not detected in the coral during the... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_3790390" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Vibrio shiloi , the causative agent of bleaching of the coral Oculina patagonica in the Mediterranean Sea, is present in all bleached O. patagonica corals in the summer (25-30 ∞ ∞ ∞ ∞ C), but can be not detected in the coral during the winter (16-20 ∞ ∞ ∞ ∞ C). Furthermore, the pathogen can not survive in O. patagonica at temperatures below 20 ∞ ∞ ∞ ∞ C. Using fluorescence in situ hybridization (FISH) with a V. shiloi -specific oligonucleotide probe, we found that the marine fireworm Hermodice caranculata is a winter reservoir for V. shiloi . Worms taken directly from the sea during the winter contained ~ 10 8 V. shiloi per worm by FISH analysis. However, colony-forming units (cfu) revealed only 4.1-18.3 ¥ ¥ ¥ ¥ 10 4 V. shiloi per worm, indicating that ~ 99.9% of them were in the viable-but-not-culturable (VBNC) state. When worms were infected with V. shiloi , most of the bacteria adhered to the worm within 24 h and then penetrated into epidermal cells. By 48 h, less than 10 ----4 of the intact V. shiloi in the worm gave rise to colonies, suggesting that they differentiated inside the worm into the VBNC state. When worms infected with V. shiloi were placed in aquaria containing O. patagonica , all of the corals showed small patches of bleached tissue in 7-10 days and total bleaching in 17 days. This is the first report of a reservoir and vector for a coral disease.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/3790390" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="31820a1f72278e286717ea59e3e3beca" rel="nofollow" data-download="{"attachment_id":50134050,"asset_id":3790390,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/50134050/download_file?st=MTc0MDU3NTMzMyw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="4659422" href="https://biu.academia.edu/MaozFine">Maoz Fine</a><script data-card-contents-for-user="4659422" type="text/json">{"id":4659422,"first_name":"Maoz","last_name":"Fine","domain_name":"biu","page_name":"MaozFine","display_name":"Maoz Fine","profile_url":"https://biu.academia.edu/MaozFine?f_ri=1444","photo":"https://0.academia-photos.com/4659422/15792081/16317117/s65_maoz.fine.jpg"}</script></span></span></li><li class="js-paper-rank-work_3790390 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="3790390"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 3790390, container: ".js-paper-rank-work_3790390", }); });</script></li><li class="js-percentile-work_3790390 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 3790390; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_3790390"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_3790390 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="3790390"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 3790390; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=3790390]").text(description); $(".js-view-count-work_3790390").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_3790390").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="3790390"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">9</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="159" rel="nofollow" href="https://www.academia.edu/Documents/in/Microbiology">Microbiology</a>, <script data-card-contents-for-ri="159" type="text/json">{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="1444" rel="nofollow" href="https://www.academia.edu/Documents/in/Environmental_microbiology">Environmental microbiology</a>, <script data-card-contents-for-ri="1444" type="text/json">{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="65882" rel="nofollow" href="https://www.academia.edu/Documents/in/Fluorescence_in_situ_hybridization">Fluorescence in situ hybridization</a>, <script data-card-contents-for-ri="65882" type="text/json">{"id":65882,"name":"Fluorescence in situ hybridization","url":"https://www.academia.edu/Documents/in/Fluorescence_in_situ_hybridization?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="72878" rel="nofollow" href="https://www.academia.edu/Documents/in/Coral_Bleaching">Coral Bleaching</a><script data-card-contents-for-ri="72878" type="text/json">{"id":72878,"name":"Coral Bleaching","url":"https://www.academia.edu/Documents/in/Coral_Bleaching?f_ri=1444","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=3790390]'), work: {"id":3790390,"title":"The marine fireworm Hermodice carunculata is a winter reservoir and spring-summer vector for the coral-bleaching pathogen Vibrio shiloi","created_at":"2013-06-25T18:36:29.478-07:00","url":"https://www.academia.edu/3790390/The_marine_fireworm_Hermodice_carunculata_is_a_winter_reservoir_and_spring_summer_vector_for_the_coral_bleaching_pathogen_Vibrio_shiloi?f_ri=1444","dom_id":"work_3790390","summary":"Vibrio shiloi , the causative agent of bleaching of the coral Oculina patagonica in the Mediterranean Sea, is present in all bleached O. patagonica corals in the summer (25-30 ∞ ∞ ∞ ∞ C), but can be not detected in the coral during the winter (16-20 ∞ ∞ ∞ ∞ C). Furthermore, the pathogen can not survive in O. patagonica at temperatures below 20 ∞ ∞ ∞ ∞ C. Using fluorescence in situ hybridization (FISH) with a V. shiloi -specific oligonucleotide probe, we found that the marine fireworm Hermodice caranculata is a winter reservoir for V. shiloi . Worms taken directly from the sea during the winter contained ~ 10 8 V. shiloi per worm by FISH analysis. However, colony-forming units (cfu) revealed only 4.1-18.3 ¥ ¥ ¥ ¥ 10 4 V. shiloi per worm, indicating that ~ 99.9% of them were in the viable-but-not-culturable (VBNC) state. When worms were infected with V. shiloi , most of the bacteria adhered to the worm within 24 h and then penetrated into epidermal cells. By 48 h, less than 10 ----4 of the intact V. shiloi in the worm gave rise to colonies, suggesting that they differentiated inside the worm into the VBNC state. When worms infected with V. shiloi were placed in aquaria containing O. patagonica , all of the corals showed small patches of bleached tissue in 7-10 days and total bleaching in 17 days. This is the first report of a reservoir and vector for a coral disease.","downloadable_attachments":[{"id":50134050,"asset_id":3790390,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":4659422,"first_name":"Maoz","last_name":"Fine","domain_name":"biu","page_name":"MaozFine","display_name":"Maoz Fine","profile_url":"https://biu.academia.edu/MaozFine?f_ri=1444","photo":"https://0.academia-photos.com/4659422/15792081/16317117/s65_maoz.fine.jpg"}],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=1444","nofollow":true},{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true},{"id":65882,"name":"Fluorescence in situ hybridization","url":"https://www.academia.edu/Documents/in/Fluorescence_in_situ_hybridization?f_ri=1444","nofollow":true},{"id":72878,"name":"Coral Bleaching","url":"https://www.academia.edu/Documents/in/Coral_Bleaching?f_ri=1444","nofollow":true},{"id":77753,"name":"Anthozoa","url":"https://www.academia.edu/Documents/in/Anthozoa?f_ri=1444"},{"id":93736,"name":"Polychaeta","url":"https://www.academia.edu/Documents/in/Polychaeta?f_ri=1444"},{"id":133177,"name":"Temperature","url":"https://www.academia.edu/Documents/in/Temperature?f_ri=1444"},{"id":379748,"name":"Vibrio","url":"https://www.academia.edu/Documents/in/Vibrio?f_ri=1444"},{"id":1133132,"name":"Environmental","url":"https://www.academia.edu/Documents/in/Environmental?f_ri=1444"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_67518586" data-work_id="67518586" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/67518586/Biofilm_building_capacity_of_Salmonella_enterica_strains_from_the_poultry_farm_environment">Biofilm building capacity of Salmonella enterica strains from the poultry farm environment</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">The Biofilm (BF) building capacity of different serotypes of Salmonella enterica derived from the poultry farm environment was investigated. Starting point for the investigation was the question if farm-isolated Salmonella serotypes with... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_67518586" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">The Biofilm (BF) building capacity of different serotypes of Salmonella enterica derived from the poultry farm environment was investigated. Starting point for the investigation was the question if farm-isolated Salmonella serotypes with high importance for poultry meat and egg production are capable of forming a BF under defined laboratory conditions. Several isolates from different stages of the production cycle were chosen and compared to laboratory grown strains of the same serotype. BF building capacity was analyzed in a 96-well format during a time period of 2 days. Pulse field gel electrophoresis was used to establish a relationship between different isolates. The BF building capacity of a monospecies BF was strongly dependent on the temperature used for incubation. Results indicated further that certain farm isolates were capable of forming BF under laboratory conditions, whereas laboratory grown strains were not. Considerable differences between different field serovars and within one serovar exist. In conclusion, the BF building capacity of poultry-derived isolates is a function of adaptation to their host environment. Thus, the control of BF as a reservoir for Salmonella in the farm environment is of crucial importance for the overall improvement of food safety. Mechanical and substance-based approaches for this control exist in several variations, but overall decontamination success is difficult to achieve and needs to be especially adapted to the farm environment.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/67518586" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="6884b7fc3a73d99d2327b5e498ff3921" rel="nofollow" data-download="{"attachment_id":78303675,"asset_id":67518586,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/78303675/download_file?st=MTc0MDU3NTMzMyw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="37813590" href="https://independent.academia.edu/HafezHafez1">Hafez Hafez</a><script data-card-contents-for-user="37813590" type="text/json">{"id":37813590,"first_name":"Hafez","last_name":"Hafez","domain_name":"independent","page_name":"HafezHafez1","display_name":"Hafez Hafez","profile_url":"https://independent.academia.edu/HafezHafez1?f_ri=1444","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_67518586 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="67518586"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 67518586, container: ".js-paper-rank-work_67518586", }); });</script></li><li class="js-percentile-work_67518586 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 67518586; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_67518586"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_67518586 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="67518586"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 67518586; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=67518586]").text(description); $(".js-view-count-work_67518586").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_67518586").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="67518586"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">13</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="1290" rel="nofollow" href="https://www.academia.edu/Documents/in/Immunology">Immunology</a>, <script data-card-contents-for-ri="1290" type="text/json">{"id":1290,"name":"Immunology","url":"https://www.academia.edu/Documents/in/Immunology?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="1444" rel="nofollow" href="https://www.academia.edu/Documents/in/Environmental_microbiology">Environmental microbiology</a>, <script data-card-contents-for-ri="1444" type="text/json">{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="2187" rel="nofollow" href="https://www.academia.edu/Documents/in/Biofilms">Biofilms</a>, <script data-card-contents-for-ri="2187" type="text/json">{"id":2187,"name":"Biofilms","url":"https://www.academia.edu/Documents/in/Biofilms?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="6947" rel="nofollow" href="https://www.academia.edu/Documents/in/Medical_Microbiology">Medical Microbiology</a><script data-card-contents-for-ri="6947" type="text/json">{"id":6947,"name":"Medical Microbiology","url":"https://www.academia.edu/Documents/in/Medical_Microbiology?f_ri=1444","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=67518586]'), work: {"id":67518586,"title":"Biofilm building capacity of Salmonella enterica strains from the poultry farm environment","created_at":"2022-01-07T05:07:20.901-08:00","url":"https://www.academia.edu/67518586/Biofilm_building_capacity_of_Salmonella_enterica_strains_from_the_poultry_farm_environment?f_ri=1444","dom_id":"work_67518586","summary":"The Biofilm (BF) building capacity of different serotypes of Salmonella enterica derived from the poultry farm environment was investigated. Starting point for the investigation was the question if farm-isolated Salmonella serotypes with high importance for poultry meat and egg production are capable of forming a BF under defined laboratory conditions. Several isolates from different stages of the production cycle were chosen and compared to laboratory grown strains of the same serotype. BF building capacity was analyzed in a 96-well format during a time period of 2 days. Pulse field gel electrophoresis was used to establish a relationship between different isolates. The BF building capacity of a monospecies BF was strongly dependent on the temperature used for incubation. Results indicated further that certain farm isolates were capable of forming BF under laboratory conditions, whereas laboratory grown strains were not. Considerable differences between different field serovars and within one serovar exist. In conclusion, the BF building capacity of poultry-derived isolates is a function of adaptation to their host environment. Thus, the control of BF as a reservoir for Salmonella in the farm environment is of crucial importance for the overall improvement of food safety. Mechanical and substance-based approaches for this control exist in several variations, but overall decontamination success is difficult to achieve and needs to be especially adapted to the farm environment.","downloadable_attachments":[{"id":78303675,"asset_id":67518586,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":37813590,"first_name":"Hafez","last_name":"Hafez","domain_name":"independent","page_name":"HafezHafez1","display_name":"Hafez Hafez","profile_url":"https://independent.academia.edu/HafezHafez1?f_ri=1444","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":1290,"name":"Immunology","url":"https://www.academia.edu/Documents/in/Immunology?f_ri=1444","nofollow":true},{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true},{"id":2187,"name":"Biofilms","url":"https://www.academia.edu/Documents/in/Biofilms?f_ri=1444","nofollow":true},{"id":6947,"name":"Medical Microbiology","url":"https://www.academia.edu/Documents/in/Medical_Microbiology?f_ri=1444","nofollow":true},{"id":61157,"name":"Molecular typing","url":"https://www.academia.edu/Documents/in/Molecular_typing?f_ri=1444"},{"id":65788,"name":"Biofilm","url":"https://www.academia.edu/Documents/in/Biofilm?f_ri=1444"},{"id":255899,"name":"Poultry","url":"https://www.academia.edu/Documents/in/Poultry?f_ri=1444"},{"id":372410,"name":"Genotype","url":"https://www.academia.edu/Documents/in/Genotype?f_ri=1444"},{"id":515695,"name":"Pulsed field gel electrophoresis (PFGE)","url":"https://www.academia.edu/Documents/in/Pulsed_field_gel_electrophoresis_PFGE_?f_ri=1444"},{"id":875567,"name":"Poultry Production","url":"https://www.academia.edu/Documents/in/Poultry_Production?f_ri=1444"},{"id":2032368,"name":"Serotyping","url":"https://www.academia.edu/Documents/in/Serotyping?f_ri=1444"},{"id":3088872,"name":"Salmonella enterica","url":"https://www.academia.edu/Documents/in/Salmonella_enterica?f_ri=1444"},{"id":3789879,"name":"Cardiovascular medicine and haematology","url":"https://www.academia.edu/Documents/in/Cardiovascular_medicine_and_haematology?f_ri=1444"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_50578827" data-work_id="50578827" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/50578827/Disturbance_opens_recruitment_sites_for_bacterial_colonization">Disturbance opens recruitment sites for bacterial colonization</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Little is known about the role of immigration in shaping bacterial communities or the factors that may dictate success or failure of colonization by bacteria from regional species pools. To address these knowledge gaps, the influence of... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_50578827" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Little is known about the role of immigration in shaping bacterial communities or the factors that may dictate success or failure of colonization by bacteria from regional species pools. To address these knowledge gaps, the influence of bacterial colonization into an ecosystem (activated sludge bioreactor) was measured through a disturbance gradient (successive decreases in the parameter solids retention time) relative to stable operational conditions. Through a DNA sequencing approach, we show that the most abundant bacteria within the immigrant community have a greater probability of colonizing the receiving ecosystem, but mostly as low abundance community members. Only during the disturbance do some of these bacterial populations significantly increase in abundance beyond background levels and in few cases become dominant community members post-disturbance. Two mechanisms facilitate the enhanced enrichment of immigrant populations during disturbance: 1) the availability of resour...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/50578827" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="d82c635ee9cd1501bb9c6e442846135b" rel="nofollow" data-download="{"attachment_id":68507334,"asset_id":50578827,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/68507334/download_file?st=MTc0MDU3NTMzMyw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="37025092" href="https://mines.academia.edu/JunkoMunakataMarr">Junko Munakata Marr</a><script data-card-contents-for-user="37025092" type="text/json">{"id":37025092,"first_name":"Junko","last_name":"Munakata Marr","domain_name":"mines","page_name":"JunkoMunakataMarr","display_name":"Junko Munakata Marr","profile_url":"https://mines.academia.edu/JunkoMunakataMarr?f_ri=1444","photo":"https://gravatar.com/avatar/f531fbdc7cbe0ba585791f571b51563b?s=65"}</script></span></span></li><li class="js-paper-rank-work_50578827 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="50578827"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 50578827, container: ".js-paper-rank-work_50578827", }); });</script></li><li class="js-percentile-work_50578827 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 50578827; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_50578827"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_50578827 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="50578827"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 50578827; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=50578827]").text(description); $(".js-view-count-work_50578827").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_50578827").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="50578827"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">7</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="159" rel="nofollow" href="https://www.academia.edu/Documents/in/Microbiology">Microbiology</a>, <script data-card-contents-for-ri="159" type="text/json">{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="1444" rel="nofollow" href="https://www.academia.edu/Documents/in/Environmental_microbiology">Environmental microbiology</a>, <script data-card-contents-for-ri="1444" type="text/json">{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="373754" rel="nofollow" href="https://www.academia.edu/Documents/in/Ecosystem">Ecosystem</a>, <script data-card-contents-for-ri="373754" type="text/json">{"id":373754,"name":"Ecosystem","url":"https://www.academia.edu/Documents/in/Ecosystem?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="410387" rel="nofollow" href="https://www.academia.edu/Documents/in/Sewage">Sewage</a><script data-card-contents-for-ri="410387" type="text/json">{"id":410387,"name":"Sewage","url":"https://www.academia.edu/Documents/in/Sewage?f_ri=1444","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=50578827]'), work: {"id":50578827,"title":"Disturbance opens recruitment sites for bacterial colonization","created_at":"2021-08-02T07:26:53.428-07:00","url":"https://www.academia.edu/50578827/Disturbance_opens_recruitment_sites_for_bacterial_colonization?f_ri=1444","dom_id":"work_50578827","summary":"Little is known about the role of immigration in shaping bacterial communities or the factors that may dictate success or failure of colonization by bacteria from regional species pools. To address these knowledge gaps, the influence of bacterial colonization into an ecosystem (activated sludge bioreactor) was measured through a disturbance gradient (successive decreases in the parameter solids retention time) relative to stable operational conditions. Through a DNA sequencing approach, we show that the most abundant bacteria within the immigrant community have a greater probability of colonizing the receiving ecosystem, but mostly as low abundance community members. Only during the disturbance do some of these bacterial populations significantly increase in abundance beyond background levels and in few cases become dominant community members post-disturbance. Two mechanisms facilitate the enhanced enrichment of immigrant populations during disturbance: 1) the availability of resour...","downloadable_attachments":[{"id":68507334,"asset_id":50578827,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":37025092,"first_name":"Junko","last_name":"Munakata Marr","domain_name":"mines","page_name":"JunkoMunakataMarr","display_name":"Junko Munakata Marr","profile_url":"https://mines.academia.edu/JunkoMunakataMarr?f_ri=1444","photo":"https://gravatar.com/avatar/f531fbdc7cbe0ba585791f571b51563b?s=65"}],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=1444","nofollow":true},{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true},{"id":373754,"name":"Ecosystem","url":"https://www.academia.edu/Documents/in/Ecosystem?f_ri=1444","nofollow":true},{"id":410387,"name":"Sewage","url":"https://www.academia.edu/Documents/in/Sewage?f_ri=1444","nofollow":true},{"id":501201,"name":"Bioreactors","url":"https://www.academia.edu/Documents/in/Bioreactors?f_ri=1444"},{"id":537754,"name":"Microbiota","url":"https://www.academia.edu/Documents/in/Microbiota?f_ri=1444"},{"id":2235564,"name":"Proteobacteria","url":"https://www.academia.edu/Documents/in/Proteobacteria?f_ri=1444"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_48312553" data-work_id="48312553" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/48312553/Detection_of_antibiotic_resistant_bacteria_inhabiting_the_sand_of_non_recreational_marine_beach">Detection of antibiotic resistant bacteria inhabiting the sand of non-recreational marine beach</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">The present study examined the antibiotic resistance of heterotrophic bacteria, which were isolated from the sand of the beach located in the National Park of the southern Baltic Sea coast. The bacteria demonstrated low levels of... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_48312553" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">The present study examined the antibiotic resistance of heterotrophic bacteria, which were isolated from the sand of the beach located in the National Park of the southern Baltic Sea coast. The bacteria demonstrated low levels of antibiotic resistance. These microorganisms were the most resistant to cefaclor and clindamycin and the most sensitive to clarithromycin, doxycycline, gentamycin and oxytetracycline. The majority of bacteria inhabiting the sand of the studied beach were resistant to only one antibiotic out of 18 tested antibiotics in this study. The bacteria inhabiting the middle part of the beach and the dune were more antibiotic resistant than bacteria isolated from the seawater and the shoreline-seawater contact zone. Generally, there was no significant difference in antibiotic resistance between bacteria isolated from the surface and the subsurface sand layers. The bacterial antibiotic resistance level depends on the chemical structure of antibiotics.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/48312553" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="4d920c4a0f2c9f4b5dea19c341fcae9b" rel="nofollow" data-download="{"attachment_id":66991727,"asset_id":48312553,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/66991727/download_file?st=MTc0MDU3NTMzMyw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="133364573" href="https://independent.academia.edu/ZMudryk">Zbigniew Mudryk</a><script data-card-contents-for-user="133364573" type="text/json">{"id":133364573,"first_name":"Zbigniew","last_name":"Mudryk","domain_name":"independent","page_name":"ZMudryk","display_name":"Zbigniew Mudryk","profile_url":"https://independent.academia.edu/ZMudryk?f_ri=1444","photo":"https://0.academia-photos.com/133364573/36593307/31279006/s65_zbigniew.mudryk.jpg"}</script></span></span></li><li class="js-paper-rank-work_48312553 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="48312553"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 48312553, container: ".js-paper-rank-work_48312553", }); });</script></li><li class="js-percentile-work_48312553 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 48312553; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_48312553"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_48312553 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="48312553"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 48312553; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=48312553]").text(description); $(".js-view-count-work_48312553").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_48312553").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="48312553"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">16</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="1444" rel="nofollow" href="https://www.academia.edu/Documents/in/Environmental_microbiology">Environmental microbiology</a>, <script data-card-contents-for-ri="1444" type="text/json">{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="21724" rel="nofollow" href="https://www.academia.edu/Documents/in/Water_Pollution">Water Pollution</a>, <script data-card-contents-for-ri="21724" type="text/json">{"id":21724,"name":"Water Pollution","url":"https://www.academia.edu/Documents/in/Water_Pollution?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="28235" rel="nofollow" href="https://www.academia.edu/Documents/in/Multidisciplinary">Multidisciplinary</a>, <script data-card-contents-for-ri="28235" type="text/json">{"id":28235,"name":"Multidisciplinary","url":"https://www.academia.edu/Documents/in/Multidisciplinary?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="35620" rel="nofollow" href="https://www.academia.edu/Documents/in/Antibiotic_Resistance">Antibiotic Resistance</a><script data-card-contents-for-ri="35620" type="text/json">{"id":35620,"name":"Antibiotic Resistance","url":"https://www.academia.edu/Documents/in/Antibiotic_Resistance?f_ri=1444","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=48312553]'), work: {"id":48312553,"title":"Detection of antibiotic resistant bacteria inhabiting the sand of non-recreational marine beach","created_at":"2021-05-04T13:18:18.561-07:00","url":"https://www.academia.edu/48312553/Detection_of_antibiotic_resistant_bacteria_inhabiting_the_sand_of_non_recreational_marine_beach?f_ri=1444","dom_id":"work_48312553","summary":"The present study examined the antibiotic resistance of heterotrophic bacteria, which were isolated from the sand of the beach located in the National Park of the southern Baltic Sea coast. The bacteria demonstrated low levels of antibiotic resistance. These microorganisms were the most resistant to cefaclor and clindamycin and the most sensitive to clarithromycin, doxycycline, gentamycin and oxytetracycline. The majority of bacteria inhabiting the sand of the studied beach were resistant to only one antibiotic out of 18 tested antibiotics in this study. The bacteria inhabiting the middle part of the beach and the dune were more antibiotic resistant than bacteria isolated from the seawater and the shoreline-seawater contact zone. Generally, there was no significant difference in antibiotic resistance between bacteria isolated from the surface and the subsurface sand layers. The bacterial antibiotic resistance level depends on the chemical structure of antibiotics.","downloadable_attachments":[{"id":66991727,"asset_id":48312553,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":133364573,"first_name":"Zbigniew","last_name":"Mudryk","domain_name":"independent","page_name":"ZMudryk","display_name":"Zbigniew Mudryk","profile_url":"https://independent.academia.edu/ZMudryk?f_ri=1444","photo":"https://0.academia-photos.com/133364573/36593307/31279006/s65_zbigniew.mudryk.jpg"}],"research_interests":[{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true},{"id":21724,"name":"Water Pollution","url":"https://www.academia.edu/Documents/in/Water_Pollution?f_ri=1444","nofollow":true},{"id":28235,"name":"Multidisciplinary","url":"https://www.academia.edu/Documents/in/Multidisciplinary?f_ri=1444","nofollow":true},{"id":35620,"name":"Antibiotic Resistance","url":"https://www.academia.edu/Documents/in/Antibiotic_Resistance?f_ri=1444","nofollow":true},{"id":113903,"name":"Bacteria","url":"https://www.academia.edu/Documents/in/Bacteria?f_ri=1444"},{"id":224515,"name":"ARENA","url":"https://www.academia.edu/Documents/in/ARENA?f_ri=1444"},{"id":284869,"name":"National Park","url":"https://www.academia.edu/Documents/in/National_Park?f_ri=1444"},{"id":335984,"name":"Anti-Bacterial Agents","url":"https://www.academia.edu/Documents/in/Anti-Bacterial_Agents?f_ri=1444"},{"id":340096,"name":"Contact Zone","url":"https://www.academia.edu/Documents/in/Contact_Zone?f_ri=1444"},{"id":350162,"name":"SAND","url":"https://www.academia.edu/Documents/in/SAND?f_ri=1444"},{"id":503268,"name":"Heterotrophy","url":"https://www.academia.edu/Documents/in/Heterotrophy?f_ri=1444"},{"id":1031069,"name":"Silicon Dioxide","url":"https://www.academia.edu/Documents/in/Silicon_Dioxide?f_ri=1444"},{"id":1431633,"name":"Chemical Structure","url":"https://www.academia.edu/Documents/in/Chemical_Structure?f_ri=1444"},{"id":1751767,"name":"Recreational Activity","url":"https://www.academia.edu/Documents/in/Recreational_Activity?f_ri=1444"},{"id":1833948,"name":"Playa","url":"https://www.academia.edu/Documents/in/Playa?f_ri=1444"},{"id":2563914,"name":"Heterotrophic Bacteria","url":"https://www.academia.edu/Documents/in/Heterotrophic_Bacteria?f_ri=1444"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_44375909" data-work_id="44375909" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/44375909/Chapter_14_Perspectives_in_desulfurization_of_coal_using_microbes">Chapter 14 — Perspectives in desulfurization of coal using microbes</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Coal is one of the most commonly utilized fossil fuel for energy production across the globe leading to higher amount of emission of sulfur dioxide (SO2) in the atmosphere. Combustion of coal containing higher contents of sulfur is a... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_44375909" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Coal is one of the most commonly utilized fossil fuel for energy production across the globe leading to higher amount of emission of sulfur dioxide (SO2) in the atmosphere. Combustion of coal containing higher contents of sulfur is a major factor responsible for acid rain and associated environmental degradation. The currently used physico-chemical approaches for removal of sulfur from coal are expensive, toxic in nature and inappropriate for different types of coal. The application of microbial resources for removal of sulfur from coal is an ecofriendly technique and could be successfully employed at industrial scale after laboratory scale process optimization. The process of sulfur removal by microbes is carried out by enzymes and is considerably influenced by different factors including pH, temperature, nutrient composition, shaking or standing condition, coal types and most importantly type of microorganisms used. Different bacteria and fungi isolated from coal contaminated site as well as non-contaminated site exhibiting sulfur removal potential can be used as next generation tool for development of clean coal technology. Furthermore, through the application of modern genetic manipulation techniques, enhanced expression of genes responsible for desulfurization can be performed in order to accelerate the process of sulfur removal from coal. The present chapter is prepared with an aim to discuss the diverse microbial population participating in desulfurization, their efficiency, mechanism of sulfur elimination, role of important environmental factors along with possible applications and challenges in industrial scale sulfur removal.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/44375909" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="01cab0737fcaeb9c2abd3588e104bf03" rel="nofollow" data-download="{"attachment_id":64776502,"asset_id":44375909,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/64776502/download_file?st=MTc0MDU3NTMzMyw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="3299240" href="https://jhusom.academia.edu/KinIsraelNotarte">Kin Israel Notarte</a><script data-card-contents-for-user="3299240" type="text/json">{"id":3299240,"first_name":"Kin Israel","last_name":"Notarte","domain_name":"jhusom","page_name":"KinIsraelNotarte","display_name":"Kin Israel Notarte","profile_url":"https://jhusom.academia.edu/KinIsraelNotarte?f_ri=1444","photo":"https://0.academia-photos.com/3299240/1097761/18750191/s65_kin_israel.notarte.jpg"}</script></span></span></li><li class="js-paper-rank-work_44375909 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="44375909"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 44375909, container: ".js-paper-rank-work_44375909", }); });</script></li><li class="js-percentile-work_44375909 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 44375909; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_44375909"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_44375909 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="44375909"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 44375909; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=44375909]").text(description); $(".js-view-count-work_44375909").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_44375909").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="44375909"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">4</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="1444" rel="nofollow" href="https://www.academia.edu/Documents/in/Environmental_microbiology">Environmental microbiology</a>, <script data-card-contents-for-ri="1444" type="text/json">{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="6333" rel="nofollow" href="https://www.academia.edu/Documents/in/Bioremediation">Bioremediation</a>, <script data-card-contents-for-ri="6333" type="text/json">{"id":6333,"name":"Bioremediation","url":"https://www.academia.edu/Documents/in/Bioremediation?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="10745" rel="nofollow" href="https://www.academia.edu/Documents/in/Clean_Coal_Technologies">Clean Coal Technologies</a>, <script data-card-contents-for-ri="10745" type="text/json">{"id":10745,"name":"Clean Coal Technologies","url":"https://www.academia.edu/Documents/in/Clean_Coal_Technologies?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="81231" rel="nofollow" href="https://www.academia.edu/Documents/in/Environmental_soil_and_water_pollution_prevention_and_monitoring">Environmental (soil and water) pollution prevention and monitoring.</a><script data-card-contents-for-ri="81231" type="text/json">{"id":81231,"name":"Environmental (soil and water) pollution prevention and monitoring.","url":"https://www.academia.edu/Documents/in/Environmental_soil_and_water_pollution_prevention_and_monitoring?f_ri=1444","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=44375909]'), work: {"id":44375909,"title":"Chapter 14 — Perspectives in desulfurization of coal using microbes","created_at":"2020-10-26T15:02:37.037-07:00","url":"https://www.academia.edu/44375909/Chapter_14_Perspectives_in_desulfurization_of_coal_using_microbes?f_ri=1444","dom_id":"work_44375909","summary":"Coal is one of the most commonly utilized fossil fuel for energy production across the globe leading to higher amount of emission of sulfur dioxide (SO2) in the atmosphere. Combustion of coal containing higher contents of sulfur is a major factor responsible for acid rain and associated environmental degradation. The currently used physico-chemical approaches for removal of sulfur from coal are expensive, toxic in nature and inappropriate for different types of coal. The application of microbial resources for removal of sulfur from coal is an ecofriendly technique and could be successfully employed at industrial scale after laboratory scale process optimization. The process of sulfur removal by microbes is carried out by enzymes and is considerably influenced by different factors including pH, temperature, nutrient composition, shaking or standing condition, coal types and most importantly type of microorganisms used. Different bacteria and fungi isolated from coal contaminated site as well as non-contaminated site exhibiting sulfur removal potential can be used as next generation tool for development of clean coal technology. Furthermore, through the application of modern genetic manipulation techniques, enhanced expression of genes responsible for desulfurization can be performed in order to accelerate the process of sulfur removal from coal. The present chapter is prepared with an aim to discuss the diverse microbial population participating in desulfurization, their efficiency, mechanism of sulfur elimination, role of important environmental factors along with possible applications and challenges in industrial scale sulfur removal.","downloadable_attachments":[{"id":64776502,"asset_id":44375909,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":3299240,"first_name":"Kin Israel","last_name":"Notarte","domain_name":"jhusom","page_name":"KinIsraelNotarte","display_name":"Kin Israel Notarte","profile_url":"https://jhusom.academia.edu/KinIsraelNotarte?f_ri=1444","photo":"https://0.academia-photos.com/3299240/1097761/18750191/s65_kin_israel.notarte.jpg"}],"research_interests":[{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true},{"id":6333,"name":"Bioremediation","url":"https://www.academia.edu/Documents/in/Bioremediation?f_ri=1444","nofollow":true},{"id":10745,"name":"Clean Coal Technologies","url":"https://www.academia.edu/Documents/in/Clean_Coal_Technologies?f_ri=1444","nofollow":true},{"id":81231,"name":"Environmental (soil and water) pollution prevention and monitoring.","url":"https://www.academia.edu/Documents/in/Environmental_soil_and_water_pollution_prevention_and_monitoring?f_ri=1444","nofollow":true}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_74105513" data-work_id="74105513" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/74105513/Purification_and_characterization_of_alcohol_oxidase_from_Paecilomyces_variotii_isolated_as_a_formaldehyde_resistant_fungus">Purification and characterization of alcohol oxidase from Paecilomyces variotii isolated as a formaldehyde-resistant fungus</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Paecilomyces variotii IRI017 was isolated as a formaldehyde-resistant fungus from wastewater containing formaldehyde. The fungus grew in a medium containing 0.5% formaldehyde and had consumed formaldehyde completely after 5 days. Alcohol... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_74105513" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Paecilomyces variotii IRI017 was isolated as a formaldehyde-resistant fungus from wastewater containing formaldehyde. The fungus grew in a medium containing 0.5% formaldehyde and had consumed formaldehyde completely after 5 days. Alcohol oxidase was purified from the fungus grown on methanol. A 20-fold purification was achieved with a yield of 44%. The molecular mass of the purified enzyme was estimated to be 73 and 450 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and gel filtration chromatography, respectively, suggesting that the enzyme consists of six identical subunits. The Nterminal amino acid sequence of the subunit was TIPDEV-DIII. The enzyme showed an absorption spectrum typical of a flavoprotein and had a noncovalently bound flavin different from FAD, FMN, and riboflavin. The pH optimum of the enzyme activity was pH 6-10. The enzyme was stable in the pH range of pH 5-10. The enzyme retained full activity after incubation at 50°C for 30 min. The enzyme oxidized not only methanol but also lower primary alcohols and formaldehyde. The K m values for methanol, ethanol, and formaldehyde were 1.9, 3.8, and 4.9 mmol l −1 , respectively.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/74105513" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="c082775d14f04270f151fec771ab837a" rel="nofollow" data-download="{"attachment_id":82377412,"asset_id":74105513,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/82377412/download_file?st=MTc0MDU3NTMzMyw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="34210695" href="https://independent.academia.edu/MykhailoGonchar">Mykhailo Gonchar</a><script data-card-contents-for-user="34210695" type="text/json">{"id":34210695,"first_name":"Mykhailo","last_name":"Gonchar","domain_name":"independent","page_name":"MykhailoGonchar","display_name":"Mykhailo Gonchar","profile_url":"https://independent.academia.edu/MykhailoGonchar?f_ri=1444","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_74105513 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="74105513"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 74105513, container: ".js-paper-rank-work_74105513", }); 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$(".js-view-count[data-work-id=74105513]").text(description); $(".js-view-count-work_74105513").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_74105513").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="74105513"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">20</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="1444" rel="nofollow" href="https://www.academia.edu/Documents/in/Environmental_microbiology">Environmental microbiology</a>, <script data-card-contents-for-ri="1444" type="text/json">{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="4987" rel="nofollow" href="https://www.academia.edu/Documents/in/Kinetics">Kinetics</a>, <script data-card-contents-for-ri="4987" type="text/json">{"id":4987,"name":"Kinetics","url":"https://www.academia.edu/Documents/in/Kinetics?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="28235" rel="nofollow" href="https://www.academia.edu/Documents/in/Multidisciplinary">Multidisciplinary</a>, <script data-card-contents-for-ri="28235" type="text/json">{"id":28235,"name":"Multidisciplinary","url":"https://www.academia.edu/Documents/in/Multidisciplinary?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="133177" rel="nofollow" href="https://www.academia.edu/Documents/in/Temperature">Temperature</a><script data-card-contents-for-ri="133177" type="text/json">{"id":133177,"name":"Temperature","url":"https://www.academia.edu/Documents/in/Temperature?f_ri=1444","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=74105513]'), work: {"id":74105513,"title":"Purification and characterization of alcohol oxidase from Paecilomyces variotii isolated as a formaldehyde-resistant fungus","created_at":"2022-03-19T21:51:14.324-07:00","url":"https://www.academia.edu/74105513/Purification_and_characterization_of_alcohol_oxidase_from_Paecilomyces_variotii_isolated_as_a_formaldehyde_resistant_fungus?f_ri=1444","dom_id":"work_74105513","summary":"Paecilomyces variotii IRI017 was isolated as a formaldehyde-resistant fungus from wastewater containing formaldehyde. The fungus grew in a medium containing 0.5% formaldehyde and had consumed formaldehyde completely after 5 days. Alcohol oxidase was purified from the fungus grown on methanol. A 20-fold purification was achieved with a yield of 44%. The molecular mass of the purified enzyme was estimated to be 73 and 450 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and gel filtration chromatography, respectively, suggesting that the enzyme consists of six identical subunits. The Nterminal amino acid sequence of the subunit was TIPDEV-DIII. The enzyme showed an absorption spectrum typical of a flavoprotein and had a noncovalently bound flavin different from FAD, FMN, and riboflavin. The pH optimum of the enzyme activity was pH 6-10. The enzyme was stable in the pH range of pH 5-10. The enzyme retained full activity after incubation at 50°C for 30 min. The enzyme oxidized not only methanol but also lower primary alcohols and formaldehyde. The K m values for methanol, ethanol, and formaldehyde were 1.9, 3.8, and 4.9 mmol l −1 , respectively.","downloadable_attachments":[{"id":82377412,"asset_id":74105513,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":34210695,"first_name":"Mykhailo","last_name":"Gonchar","domain_name":"independent","page_name":"MykhailoGonchar","display_name":"Mykhailo Gonchar","profile_url":"https://independent.academia.edu/MykhailoGonchar?f_ri=1444","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true},{"id":4987,"name":"Kinetics","url":"https://www.academia.edu/Documents/in/Kinetics?f_ri=1444","nofollow":true},{"id":28235,"name":"Multidisciplinary","url":"https://www.academia.edu/Documents/in/Multidisciplinary?f_ri=1444","nofollow":true},{"id":133177,"name":"Temperature","url":"https://www.academia.edu/Documents/in/Temperature?f_ri=1444","nofollow":true},{"id":178351,"name":"Spectrophotometry","url":"https://www.academia.edu/Documents/in/Spectrophotometry?f_ri=1444"},{"id":193464,"name":"Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis","url":"https://www.academia.edu/Documents/in/Sodium_Dodecyl_Sulfate-Polyacrylamide_Gel_Electrophoresis?f_ri=1444"},{"id":231661,"name":"Enzyme","url":"https://www.academia.edu/Documents/in/Enzyme?f_ri=1444"},{"id":269171,"name":"Applied Microbiology and Biotechnology","url":"https://www.academia.edu/Documents/in/Applied_Microbiology_and_Biotechnology?f_ri=1444"},{"id":300829,"name":"Protein Sequence Analysis","url":"https://www.academia.edu/Documents/in/Protein_Sequence_Analysis?f_ri=1444"},{"id":321836,"name":"Spectrum","url":"https://www.academia.edu/Documents/in/Spectrum?f_ri=1444"},{"id":432129,"name":"Substrate Specificity","url":"https://www.academia.edu/Documents/in/Substrate_Specificity?f_ri=1444"},{"id":583568,"name":"Enzyme activity","url":"https://www.academia.edu/Documents/in/Enzyme_activity?f_ri=1444"},{"id":585595,"name":"Molecular Mass","url":"https://www.academia.edu/Documents/in/Molecular_Mass?f_ri=1444"},{"id":695018,"name":"Molecular weight","url":"https://www.academia.edu/Documents/in/Molecular_weight?f_ri=1444"},{"id":809881,"name":"Amino Acid Sequence","url":"https://www.academia.edu/Documents/in/Amino_Acid_Sequence?f_ri=1444"},{"id":819116,"name":"Formaldehyde","url":"https://www.academia.edu/Documents/in/Formaldehyde?f_ri=1444"},{"id":975992,"name":"Polyacrylamide Gel Electrophoresis","url":"https://www.academia.edu/Documents/in/Polyacrylamide_Gel_Electrophoresis?f_ri=1444"},{"id":1268925,"name":"Flavins","url":"https://www.academia.edu/Documents/in/Flavins?f_ri=1444"},{"id":1294622,"name":"Alcohols","url":"https://www.academia.edu/Documents/in/Alcohols?f_ri=1444"},{"id":2622517,"name":"Sodium Dodecyl Sulfate","url":"https://www.academia.edu/Documents/in/Sodium_Dodecyl_Sulfate?f_ri=1444"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_40721523" data-work_id="40721523" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/40721523/Microorganisms_in_Stormwater_and_Treatment_Options_in_Developing_Countries">Microorganisms in Stormwater and Treatment Options in Developing Countries</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Microorganisms are present in stormwater. Their numbers and diversity is influenced by the microbial flora of that environment and they can come from different sources namely the air, soil and through human activity. Stormwater runoffs... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_40721523" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Microorganisms are present in stormwater. Their numbers and diversity is influenced by the microbial flora of that environment and they can come from different sources namely the air, soil and through human activity. Stormwater runoffs carry pollutants including microorganisms. Stormwater runoffs that contain organic materials and some pathogenic microorganisms when discharged into bodies of water can lead to outbreaks of infections if such receiving bodies of water are not treated to remove the microbial contaminants. Another effect of microbial presence in a stormwater runoff discharged into a body of water without treatment is the possibility of creating anoxic condition in the receiving water body. This happens when the aerobic heterotrophic bacteria in the water body multiplies thereby utilizing the dissolved oxygen in the water. The end result is the death of fish and other aquatic organisms that require oxygen for survival. Stormwater runoffs can be treated biologically through activated sludge process, trickling filter or through the oxidation pond method to reduce the organic and microbial load. The effluents resulting from such treatment can then be disinfected to destroy the remaining microorganisms and organic matter in the water. <br /><br />Keywords: stormwater, microorganisms, runoff, pollution</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/40721523" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="0367394a37ab9331efb0b628ff037f29" rel="nofollow" data-download="{"attachment_id":61007775,"asset_id":40721523,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/61007775/download_file?st=MTc0MDU3NTMzMyw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="325830" href="https://mmu.academia.edu/ErnestNnadi">Ernest Nnadi</a><script data-card-contents-for-user="325830" type="text/json">{"id":325830,"first_name":"Ernest","last_name":"Nnadi","domain_name":"mmu","page_name":"ErnestNnadi","display_name":"Ernest Nnadi","profile_url":"https://mmu.academia.edu/ErnestNnadi?f_ri=1444","photo":"https://0.academia-photos.com/325830/91631/43673482/s65_ernest.nnadi.jpg"}</script></span></span></li><li class="js-paper-rank-work_40721523 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="40721523"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 40721523, container: ".js-paper-rank-work_40721523", }); 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$(".js-view-count[data-work-id=40721523]").text(description); $(".js-view-count-work_40721523").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_40721523").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="40721523"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">16</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="55" rel="nofollow" href="https://www.academia.edu/Documents/in/Environmental_Engineering">Environmental Engineering</a>, <script data-card-contents-for-ri="55" type="text/json">{"id":55,"name":"Environmental Engineering","url":"https://www.academia.edu/Documents/in/Environmental_Engineering?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="73" rel="nofollow" href="https://www.academia.edu/Documents/in/Civil_Engineering">Civil Engineering</a>, <script data-card-contents-for-ri="73" type="text/json">{"id":73,"name":"Civil Engineering","url":"https://www.academia.edu/Documents/in/Civil_Engineering?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="402" rel="nofollow" href="https://www.academia.edu/Documents/in/Environmental_Science">Environmental Science</a>, <script data-card-contents-for-ri="402" type="text/json">{"id":402,"name":"Environmental Science","url":"https://www.academia.edu/Documents/in/Environmental_Science?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="1444" rel="nofollow" href="https://www.academia.edu/Documents/in/Environmental_microbiology">Environmental microbiology</a><script data-card-contents-for-ri="1444" type="text/json">{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=40721523]'), work: {"id":40721523,"title":"Microorganisms in Stormwater and Treatment Options in Developing Countries","created_at":"2019-10-24T11:51:58.365-07:00","url":"https://www.academia.edu/40721523/Microorganisms_in_Stormwater_and_Treatment_Options_in_Developing_Countries?f_ri=1444","dom_id":"work_40721523","summary":"\nMicroorganisms are present in stormwater. Their numbers and diversity is influenced by the microbial flora of that environment and they can come from different sources namely the air, soil and through human activity. Stormwater runoffs carry pollutants including microorganisms. Stormwater runoffs that contain organic materials and some pathogenic microorganisms when discharged into bodies of water can lead to outbreaks of infections if such receiving bodies of water are not treated to remove the microbial contaminants. Another effect of microbial presence in a stormwater runoff discharged into a body of water without treatment is the possibility of creating anoxic condition in the receiving water body. This happens when the aerobic heterotrophic bacteria in the water body multiplies thereby utilizing the dissolved oxygen in the water. The end result is the death of fish and other aquatic organisms that require oxygen for survival. Stormwater runoffs can be treated biologically through activated sludge process, trickling filter or through the oxidation pond method to reduce the organic and microbial load. The effluents resulting from such treatment can then be disinfected to destroy the remaining microorganisms and organic matter in the water. \n\nKeywords: stormwater, microorganisms, runoff, pollution\n","downloadable_attachments":[{"id":61007775,"asset_id":40721523,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":325830,"first_name":"Ernest","last_name":"Nnadi","domain_name":"mmu","page_name":"ErnestNnadi","display_name":"Ernest Nnadi","profile_url":"https://mmu.academia.edu/ErnestNnadi?f_ri=1444","photo":"https://0.academia-photos.com/325830/91631/43673482/s65_ernest.nnadi.jpg"}],"research_interests":[{"id":55,"name":"Environmental Engineering","url":"https://www.academia.edu/Documents/in/Environmental_Engineering?f_ri=1444","nofollow":true},{"id":73,"name":"Civil Engineering","url":"https://www.academia.edu/Documents/in/Civil_Engineering?f_ri=1444","nofollow":true},{"id":402,"name":"Environmental Science","url":"https://www.academia.edu/Documents/in/Environmental_Science?f_ri=1444","nofollow":true},{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true},{"id":3407,"name":"Environmental Studies","url":"https://www.academia.edu/Documents/in/Environmental_Studies?f_ri=1444"},{"id":7609,"name":"Environmental Management","url":"https://www.academia.edu/Documents/in/Environmental_Management?f_ri=1444"},{"id":9991,"name":"Wastewater Treatment","url":"https://www.academia.edu/Documents/in/Wastewater_Treatment?f_ri=1444"},{"id":18845,"name":"Environmental Sustainability","url":"https://www.academia.edu/Documents/in/Environmental_Sustainability?f_ri=1444"},{"id":21724,"name":"Water Pollution","url":"https://www.academia.edu/Documents/in/Water_Pollution?f_ri=1444"},{"id":25445,"name":"Environmental Pollution","url":"https://www.academia.edu/Documents/in/Environmental_Pollution?f_ri=1444"},{"id":37344,"name":"Earth and Environmental Sciences","url":"https://www.academia.edu/Documents/in/Earth_and_Environmental_Sciences?f_ri=1444"},{"id":50978,"name":"Irrigation \u0026 Drainage","url":"https://www.academia.edu/Documents/in/Irrigation_and_Drainage?f_ri=1444"},{"id":180994,"name":"sustainable urban drainage systems (SUDS)","url":"https://www.academia.edu/Documents/in/sustainable_urban_drainage_systems_SUDS_?f_ri=1444"},{"id":186461,"name":"Stormwater management","url":"https://www.academia.edu/Documents/in/Stormwater_management?f_ri=1444"},{"id":465094,"name":"Civil \u0026 Environmental Engineering","url":"https://www.academia.edu/Documents/in/Civil_and_Environmental_Engineering?f_ri=1444"},{"id":678575,"name":"Civil and Environmental Engineering","url":"https://www.academia.edu/Documents/in/Civil_and_Environmental_Engineering-1?f_ri=1444"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_389456 coauthored" data-work_id="389456" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/389456/Tn5090_like_Class_1_Integron_Carrying_BlaVIM_2_In_a_Pseudomonas_Putida_Strain_From_Portugal">Tn5090‐like Class 1 Integron Carrying BlaVIM‐2 In a Pseudomonas Putida Strain From Portugal</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/389456" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span 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class="u-tcGrayDark u-fw700" data-has-card-for-user="295602" href="https://aveiro.academia.edu/SoniaMendo">Sonia Mendo</a><script data-card-contents-for-user="295602" type="text/json">{"id":295602,"first_name":"Sonia","last_name":"Mendo","domain_name":"aveiro","page_name":"SoniaMendo","display_name":"Sonia Mendo","profile_url":"https://aveiro.academia.edu/SoniaMendo?f_ri=1444","photo":"https://0.academia-photos.com/295602/72898/79692/s65_sonia.mendo.jpg"}</script></span></span><span class="u-displayInlineBlock InlineList-item-text"> and <span class="u-textDecorationUnderline u-clickable InlineList-item-text js-work-more-authors-389456">+1</span><div class="hidden js-additional-users-389456"><div><span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a href="https://aveiro.academia.edu/C%C3%A1tiaSantos">Cátia Santos</a></span></div></div></span><script>(function(){ var popoverSettings = { el: $('.js-work-more-authors-389456'), placement: 'bottom', 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class="u-positionAbsolute" data-has-card-for-ri-list="389456"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">12</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="1444" rel="nofollow" href="https://www.academia.edu/Documents/in/Environmental_microbiology">Environmental microbiology</a>, <script data-card-contents-for-ri="1444" type="text/json">{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="35620" rel="nofollow" href="https://www.academia.edu/Documents/in/Antibiotic_Resistance">Antibiotic Resistance</a>, <script data-card-contents-for-ri="35620" type="text/json">{"id":35620,"name":"Antibiotic Resistance","url":"https://www.academia.edu/Documents/in/Antibiotic_Resistance?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="37772" rel="nofollow" href="https://www.academia.edu/Documents/in/Hospitals">Hospitals</a>, <script data-card-contents-for-ri="37772" type="text/json">{"id":37772,"name":"Hospitals","url":"https://www.academia.edu/Documents/in/Hospitals?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="43666" rel="nofollow" href="https://www.academia.edu/Documents/in/Clinical_Microbiology">Clinical Microbiology</a><script data-card-contents-for-ri="43666" type="text/json">{"id":43666,"name":"Clinical Microbiology","url":"https://www.academia.edu/Documents/in/Clinical_Microbiology?f_ri=1444","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=389456]'), work: {"id":389456,"title":"Tn5090‐like Class 1 Integron Carrying BlaVIM‐2 In a Pseudomonas Putida Strain From Portugal","created_at":"2010-12-13T17:48:59.969-08:00","url":"https://www.academia.edu/389456/Tn5090_like_Class_1_Integron_Carrying_BlaVIM_2_In_a_Pseudomonas_Putida_Strain_From_Portugal?f_ri=1444","dom_id":"work_389456","summary":null,"downloadable_attachments":[{"id":51427386,"asset_id":389456,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":295602,"first_name":"Sonia","last_name":"Mendo","domain_name":"aveiro","page_name":"SoniaMendo","display_name":"Sonia Mendo","profile_url":"https://aveiro.academia.edu/SoniaMendo?f_ri=1444","photo":"https://0.academia-photos.com/295602/72898/79692/s65_sonia.mendo.jpg"},{"id":8617998,"first_name":"Cátia","last_name":"Santos","domain_name":"aveiro","page_name":"CátiaSantos","display_name":"Cátia Santos","profile_url":"https://aveiro.academia.edu/C%C3%A1tiaSantos?f_ri=1444","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true},{"id":35620,"name":"Antibiotic Resistance","url":"https://www.academia.edu/Documents/in/Antibiotic_Resistance?f_ri=1444","nofollow":true},{"id":37772,"name":"Hospitals","url":"https://www.academia.edu/Documents/in/Hospitals?f_ri=1444","nofollow":true},{"id":43666,"name":"Clinical Microbiology","url":"https://www.academia.edu/Documents/in/Clinical_Microbiology?f_ri=1444","nofollow":true},{"id":44510,"name":"Portugal","url":"https://www.academia.edu/Documents/in/Portugal?f_ri=1444"},{"id":129391,"name":"Gene Order","url":"https://www.academia.edu/Documents/in/Gene_Order?f_ri=1444"},{"id":139889,"name":"Clinical microbiology and infection control","url":"https://www.academia.edu/Documents/in/Clinical_microbiology_and_infection_control?f_ri=1444"},{"id":183736,"name":"Pseudomonas putida","url":"https://www.academia.edu/Documents/in/Pseudomonas_putida?f_ri=1444"},{"id":244814,"name":"Clinical Sciences","url":"https://www.academia.edu/Documents/in/Clinical_Sciences?f_ri=1444"},{"id":295954,"name":"Integrons","url":"https://www.academia.edu/Documents/in/Integrons?f_ri=1444"},{"id":1770857,"name":"Beta Lactamases","url":"https://www.academia.edu/Documents/in/Beta_Lactamases?f_ri=1444"},{"id":2467566,"name":"Molecular Sequence Data","url":"https://www.academia.edu/Documents/in/Molecular_Sequence_Data?f_ri=1444"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_10479932" data-work_id="10479932" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/10479932/Effects_of_disinfectant_fogging_procedure_on_dust_ammonia_concentration_aerobic_bacteria_and_fungal_spores_in_a_farrowing_weaning_room">Effects of disinfectant fogging procedure on dust, ammonia concentration, aerobic bacteria and fungal spores in a farrowing-weaning room</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">M. Effects of disinfectant fogging procedure on dust, ammonia concentration, aerobic bacteria and fungal spores in a farrowing-weaning room. Abstract Introduction and Objective. In the last decades, large-scale swine production has led to... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_10479932" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">M. Effects of disinfectant fogging procedure on dust, ammonia concentration, aerobic bacteria and fungal spores in a farrowing-weaning room. Abstract Introduction and Objective. In the last decades, large-scale swine production has led to intensive rearing systems in which air quality can be easily degraded by aerial contaminants that can pose a health risk to the pigs and farm workers. This study evaluated the effects of fogging disinfectant procedure on productive performance, ammonia and dust concentration, aerobic bacteria and fungal spores spreading in the farrowing-weaning room. Materials and Method. This trial was conducted in 2 identical farrowing-weaning rooms of a piggery. In both rooms, 30 pregnant sows were lodged in individual cages. At 75 days of age, the piglets were moved to the fattening room. In the treated room, with the birth of the first suckling-pig, the fogging disinfection with diluted Virkon S was applied once a day in the experimental room per 15 minutes at 11:00. The fogging disinfectant treatment was switched between rooms at the end of the first trial period. Temperature, relative humidity, dust (TSP-RF fractions and number of particles), ammonia concentration and aerial contaminants (enterococci, Micrococcaeae and fungal spores) were monitored in both rooms. Results. Ammonia concentration reduction induced by fogging disinfection was estimated 18%, total suspended particles and the respirable fraction were significantly lower in the experimental room. Fungal spores resulted in a significant reduction by the fogging procedure, together with dust respirable fraction and fine particulate matter abatement. Conclusions. The fogging disinfection procedure improved air quality in the piggery, thereby enhancing workers and animals health.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/10479932" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="930f05ed88c8dfd04decacb15e6d73b9" rel="nofollow" data-download="{"attachment_id":47361489,"asset_id":10479932,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/47361489/download_file?st=MTc0MDU3NTMzMyw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="25712336" href="https://independent.academia.edu/AnnamariaCosta">Annamaria Costa</a><script data-card-contents-for-user="25712336" type="text/json">{"id":25712336,"first_name":"Annamaria","last_name":"Costa","domain_name":"independent","page_name":"AnnamariaCosta","display_name":"Annamaria Costa","profile_url":"https://independent.academia.edu/AnnamariaCosta?f_ri=1444","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_10479932 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="10479932"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 10479932, container: ".js-paper-rank-work_10479932", }); 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$(".js-view-count[data-work-id=10479932]").text(description); $(".js-view-count-work_10479932").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_10479932").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="10479932"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">13</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="1444" rel="nofollow" href="https://www.academia.edu/Documents/in/Environmental_microbiology">Environmental microbiology</a>, <script data-card-contents-for-ri="1444" type="text/json">{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="11801" rel="nofollow" href="https://www.academia.edu/Documents/in/Environmental_Monitoring">Environmental Monitoring</a>, <script data-card-contents-for-ri="11801" type="text/json">{"id":11801,"name":"Environmental Monitoring","url":"https://www.academia.edu/Documents/in/Environmental_Monitoring?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="17272" rel="nofollow" href="https://www.academia.edu/Documents/in/Fungi">Fungi</a>, <script data-card-contents-for-ri="17272" type="text/json">{"id":17272,"name":"Fungi","url":"https://www.academia.edu/Documents/in/Fungi?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="37881" rel="nofollow" href="https://www.academia.edu/Documents/in/Animal_Husbandry">Animal Husbandry</a><script data-card-contents-for-ri="37881" type="text/json">{"id":37881,"name":"Animal Husbandry","url":"https://www.academia.edu/Documents/in/Animal_Husbandry?f_ri=1444","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=10479932]'), work: {"id":10479932,"title":"Effects of disinfectant fogging procedure on dust, ammonia concentration, aerobic bacteria and fungal spores in a farrowing-weaning room","created_at":"2015-02-03T05:37:16.416-08:00","url":"https://www.academia.edu/10479932/Effects_of_disinfectant_fogging_procedure_on_dust_ammonia_concentration_aerobic_bacteria_and_fungal_spores_in_a_farrowing_weaning_room?f_ri=1444","dom_id":"work_10479932","summary":"M. Effects of disinfectant fogging procedure on dust, ammonia concentration, aerobic bacteria and fungal spores in a farrowing-weaning room. Abstract Introduction and Objective. In the last decades, large-scale swine production has led to intensive rearing systems in which air quality can be easily degraded by aerial contaminants that can pose a health risk to the pigs and farm workers. This study evaluated the effects of fogging disinfectant procedure on productive performance, ammonia and dust concentration, aerobic bacteria and fungal spores spreading in the farrowing-weaning room. Materials and Method. This trial was conducted in 2 identical farrowing-weaning rooms of a piggery. In both rooms, 30 pregnant sows were lodged in individual cages. At 75 days of age, the piglets were moved to the fattening room. In the treated room, with the birth of the first suckling-pig, the fogging disinfection with diluted Virkon S was applied once a day in the experimental room per 15 minutes at 11:00. The fogging disinfectant treatment was switched between rooms at the end of the first trial period. Temperature, relative humidity, dust (TSP-RF fractions and number of particles), ammonia concentration and aerial contaminants (enterococci, Micrococcaeae and fungal spores) were monitored in both rooms. Results. Ammonia concentration reduction induced by fogging disinfection was estimated 18%, total suspended particles and the respirable fraction were significantly lower in the experimental room. Fungal spores resulted in a significant reduction by the fogging procedure, together with dust respirable fraction and fine particulate matter abatement. Conclusions. The fogging disinfection procedure improved air quality in the piggery, thereby enhancing workers and animals health.","downloadable_attachments":[{"id":47361489,"asset_id":10479932,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":25712336,"first_name":"Annamaria","last_name":"Costa","domain_name":"independent","page_name":"AnnamariaCosta","display_name":"Annamaria Costa","profile_url":"https://independent.academia.edu/AnnamariaCosta?f_ri=1444","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true},{"id":11801,"name":"Environmental Monitoring","url":"https://www.academia.edu/Documents/in/Environmental_Monitoring?f_ri=1444","nofollow":true},{"id":17272,"name":"Fungi","url":"https://www.academia.edu/Documents/in/Fungi?f_ri=1444","nofollow":true},{"id":37881,"name":"Animal Husbandry","url":"https://www.academia.edu/Documents/in/Animal_Husbandry?f_ri=1444","nofollow":true},{"id":45213,"name":"Italy","url":"https://www.academia.edu/Documents/in/Italy?f_ri=1444"},{"id":58054,"name":"Environmental Sciences","url":"https://www.academia.edu/Documents/in/Environmental_Sciences?f_ri=1444"},{"id":62550,"name":"Pregnancy","url":"https://www.academia.edu/Documents/in/Pregnancy?f_ri=1444"},{"id":139817,"name":"Disinfectants","url":"https://www.academia.edu/Documents/in/Disinfectants?f_ri=1444"},{"id":219724,"name":"Ammonia","url":"https://www.academia.edu/Documents/in/Ammonia?f_ri=1444"},{"id":221185,"name":"Disinfection","url":"https://www.academia.edu/Documents/in/Disinfection?f_ri=1444"},{"id":295244,"name":"Indoor air pollution","url":"https://www.academia.edu/Documents/in/Indoor_air_pollution?f_ri=1444"},{"id":735883,"name":"Dust","url":"https://www.academia.edu/Documents/in/Dust?f_ri=1444"},{"id":993152,"name":"Sus Scrofa","url":"https://www.academia.edu/Documents/in/Sus_Scrofa?f_ri=1444"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_14301144" data-work_id="14301144" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/14301144/Ecology_and_Transmission_of_Buruli_Ulcer_Disease_A_Systematic_Review">Ecology and Transmission of Buruli Ulcer Disease: A Systematic Review</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Buruli ulcer is a neglected emerging disease that has recently been reported in some countries as the second most frequent mycobacterial disease in humans after tuberculosis. Cases have been reported from at least 32 countries in Africa... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_14301144" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Buruli ulcer is a neglected emerging disease that has recently been reported in some countries as the second most frequent mycobacterial disease in humans after tuberculosis. Cases have been reported from at least 32 countries in Africa (mainly west), Australia, Southeast Asia, China, Central and South America, and the Western Pacific. Large lesions often result in scarring, contractual deformities, amputations, and disabilities, and in Africa, most cases of the disease occur in children between the ages of 4-15 years. This environmental mycobacterium, Mycobacterium ulcerans, is found in communities associated with rivers, swamps, wetlands, and human-linked changes in the aquatic environment, particularly those created as a result of environmental disturbance such as deforestation, dam construction, and agriculture. Buruli ulcer disease is often referred to as the ''mysterious disease'' because the mode of transmission remains unclear, although several hypotheses have been proposed. The above review reveals that various routes of transmission may occur, varying amongst epidemiological setting and geographic region, and that there may be some role for living agents as reservoirs and as vectors of M. ulcerans, in particular aquatic insects, adult mosquitoes or other biting arthropods. We discuss traditional and non-traditional methods for indicting the roles of living agents as biologically significant reservoirs and/or vectors of pathogens, and suggest an intellectual framework for establishing criteria for transmission. The application of these criteria to the transmission of M. ulcerans presents a significant challenge.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/14301144" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="dfb5c0c356b8add5499d2b3932dc0949" rel="nofollow" data-download="{"attachment_id":44343021,"asset_id":14301144,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/44343021/download_file?st=MTc0MDU3NTMzMyw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="33259212" href="https://utk.academia.edu/PamelaSmall">Pamela Small</a><script data-card-contents-for-user="33259212" type="text/json">{"id":33259212,"first_name":"Pamela","last_name":"Small","domain_name":"utk","page_name":"PamelaSmall","display_name":"Pamela Small","profile_url":"https://utk.academia.edu/PamelaSmall?f_ri=1444","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_14301144 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="14301144"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 14301144, container: ".js-paper-rank-work_14301144", }); });</script></li><li class="js-percentile-work_14301144 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 14301144; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_14301144"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_14301144 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="14301144"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 14301144; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=14301144]").text(description); $(".js-view-count-work_14301144").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_14301144").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="14301144"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">12</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="1444" rel="nofollow" href="https://www.academia.edu/Documents/in/Environmental_microbiology">Environmental microbiology</a>, <script data-card-contents-for-ri="1444" type="text/json">{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="6746" rel="nofollow" href="https://www.academia.edu/Documents/in/Southeast_Asia">Southeast Asia</a>, <script data-card-contents-for-ri="6746" type="text/json">{"id":6746,"name":"Southeast Asia","url":"https://www.academia.edu/Documents/in/Southeast_Asia?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="47884" rel="nofollow" href="https://www.academia.edu/Documents/in/Biological_Sciences">Biological Sciences</a>, <script data-card-contents-for-ri="47884" type="text/json">{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="53653" rel="nofollow" href="https://www.academia.edu/Documents/in/Aquatic_Insects">Aquatic Insects</a><script data-card-contents-for-ri="53653" type="text/json">{"id":53653,"name":"Aquatic Insects","url":"https://www.academia.edu/Documents/in/Aquatic_Insects?f_ri=1444","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=14301144]'), work: {"id":14301144,"title":"Ecology and Transmission of Buruli Ulcer Disease: A Systematic Review","created_at":"2015-07-22T10:26:18.301-07:00","url":"https://www.academia.edu/14301144/Ecology_and_Transmission_of_Buruli_Ulcer_Disease_A_Systematic_Review?f_ri=1444","dom_id":"work_14301144","summary":"Buruli ulcer is a neglected emerging disease that has recently been reported in some countries as the second most frequent mycobacterial disease in humans after tuberculosis. Cases have been reported from at least 32 countries in Africa (mainly west), Australia, Southeast Asia, China, Central and South America, and the Western Pacific. Large lesions often result in scarring, contractual deformities, amputations, and disabilities, and in Africa, most cases of the disease occur in children between the ages of 4-15 years. This environmental mycobacterium, Mycobacterium ulcerans, is found in communities associated with rivers, swamps, wetlands, and human-linked changes in the aquatic environment, particularly those created as a result of environmental disturbance such as deforestation, dam construction, and agriculture. Buruli ulcer disease is often referred to as the ''mysterious disease'' because the mode of transmission remains unclear, although several hypotheses have been proposed. The above review reveals that various routes of transmission may occur, varying amongst epidemiological setting and geographic region, and that there may be some role for living agents as reservoirs and as vectors of M. ulcerans, in particular aquatic insects, adult mosquitoes or other biting arthropods. We discuss traditional and non-traditional methods for indicting the roles of living agents as biologically significant reservoirs and/or vectors of pathogens, and suggest an intellectual framework for establishing criteria for transmission. The application of these criteria to the transmission of M. ulcerans presents a significant challenge.","downloadable_attachments":[{"id":44343021,"asset_id":14301144,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":33259212,"first_name":"Pamela","last_name":"Small","domain_name":"utk","page_name":"PamelaSmall","display_name":"Pamela Small","profile_url":"https://utk.academia.edu/PamelaSmall?f_ri=1444","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true},{"id":6746,"name":"Southeast Asia","url":"https://www.academia.edu/Documents/in/Southeast_Asia?f_ri=1444","nofollow":true},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=1444","nofollow":true},{"id":53653,"name":"Aquatic Insects","url":"https://www.academia.edu/Documents/in/Aquatic_Insects?f_ri=1444","nofollow":true},{"id":62235,"name":"Tuberculosis","url":"https://www.academia.edu/Documents/in/Tuberculosis?f_ri=1444"},{"id":176798,"name":"South America","url":"https://www.academia.edu/Documents/in/South_America?f_ri=1444"},{"id":211171,"name":"Buruli Ulcer","url":"https://www.academia.edu/Documents/in/Buruli_Ulcer?f_ri=1444"},{"id":221411,"name":"Systematic review","url":"https://www.academia.edu/Documents/in/Systematic_review?f_ri=1444"},{"id":373754,"name":"Ecosystem","url":"https://www.academia.edu/Documents/in/Ecosystem?f_ri=1444"},{"id":488176,"name":"Emerging disease","url":"https://www.academia.edu/Documents/in/Emerging_disease?f_ri=1444"},{"id":546419,"name":"Age Factors","url":"https://www.academia.edu/Documents/in/Age_Factors?f_ri=1444"},{"id":801605,"name":"Mycobacterium Ulcerans","url":"https://www.academia.edu/Documents/in/Mycobacterium_Ulcerans?f_ri=1444"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_16026495" data-work_id="16026495" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/16026495/Heat_stable_toxin_production_by_strains_of_Bacillus_cereus_Bacillus_firmus_Bacillus_megaterium_Bacillus_simplex_and_Bacillus_licheniformis">Heat-stable toxin production by strains of Bacillus cereus , Bacillus firmus , Bacillus megaterium , Bacillus simplex and Bacillus licheniformis</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Strains of Bacillus cereus can produce a heat-stable toxin (cereulide). In this study, 101 Bacillus strains representing 7 Bacillus species were tested for production of heat-stable toxins. Strains of B. megaterium, B. firmus and B.... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_16026495" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Strains of Bacillus cereus can produce a heat-stable toxin (cereulide). In this study, 101 Bacillus strains representing 7 Bacillus species were tested for production of heat-stable toxins. Strains of B. megaterium, B. firmus and B. simplex were found to produce novel heat-stable toxins, which showed varying levels of toxicity. B. cereus strains (18 out of 54) were positive for toxin production. Thirteen were of serovar H1, and it was of interest that some were of clinical origin. Two were of serovars 17B and 20, which are not usually implicated in the emetic syndrome. Partial purification of the novel B. megaterium, B. simplex and B. firmus toxins showed they had similar physical characteristics to the B. cereus emetic toxin, cereulide.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/16026495" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="f042b047b3665b85949cf6484b7cbdec" rel="nofollow" data-download="{"attachment_id":42781185,"asset_id":16026495,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/42781185/download_file?st=MTc0MDU3NTMzMyw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="35166993" href="https://independent.academia.edu/KofiAidoo">Kofi Aidoo</a><script data-card-contents-for-user="35166993" type="text/json">{"id":35166993,"first_name":"Kofi","last_name":"Aidoo","domain_name":"independent","page_name":"KofiAidoo","display_name":"Kofi Aidoo","profile_url":"https://independent.academia.edu/KofiAidoo?f_ri=1444","photo":"https://gravatar.com/avatar/9f5c8bf6aabe9e4ddf6c2aae1bf7d11b?s=65"}</script></span></span></li><li class="js-paper-rank-work_16026495 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="16026495"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 16026495, container: ".js-paper-rank-work_16026495", }); });</script></li><li class="js-percentile-work_16026495 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 16026495; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_16026495"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_16026495 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="16026495"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 16026495; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=16026495]").text(description); $(".js-view-count-work_16026495").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_16026495").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="16026495"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">12</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="1444" rel="nofollow" href="https://www.academia.edu/Documents/in/Environmental_microbiology">Environmental microbiology</a>, <script data-card-contents-for-ri="1444" type="text/json">{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="31632" rel="nofollow" href="https://www.academia.edu/Documents/in/Food_Microbiology">Food Microbiology</a>, <script data-card-contents-for-ri="31632" type="text/json">{"id":31632,"name":"Food Microbiology","url":"https://www.academia.edu/Documents/in/Food_Microbiology?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="47884" rel="nofollow" href="https://www.academia.edu/Documents/in/Biological_Sciences">Biological Sciences</a>, <script data-card-contents-for-ri="47884" type="text/json">{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=1444","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="67405" rel="nofollow" href="https://www.academia.edu/Documents/in/Toxicity">Toxicity</a><script data-card-contents-for-ri="67405" type="text/json">{"id":67405,"name":"Toxicity","url":"https://www.academia.edu/Documents/in/Toxicity?f_ri=1444","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=16026495]'), work: {"id":16026495,"title":"Heat-stable toxin production by strains of Bacillus cereus , Bacillus firmus , Bacillus megaterium , Bacillus simplex and Bacillus licheniformis","created_at":"2015-09-22T06:50:35.669-07:00","url":"https://www.academia.edu/16026495/Heat_stable_toxin_production_by_strains_of_Bacillus_cereus_Bacillus_firmus_Bacillus_megaterium_Bacillus_simplex_and_Bacillus_licheniformis?f_ri=1444","dom_id":"work_16026495","summary":"Strains of Bacillus cereus can produce a heat-stable toxin (cereulide). In this study, 101 Bacillus strains representing 7 Bacillus species were tested for production of heat-stable toxins. Strains of B. megaterium, B. firmus and B. simplex were found to produce novel heat-stable toxins, which showed varying levels of toxicity. B. cereus strains (18 out of 54) were positive for toxin production. Thirteen were of serovar H1, and it was of interest that some were of clinical origin. Two were of serovars 17B and 20, which are not usually implicated in the emetic syndrome. Partial purification of the novel B. megaterium, B. simplex and B. firmus toxins showed they had similar physical characteristics to the B. cereus emetic toxin, cereulide.","downloadable_attachments":[{"id":42781185,"asset_id":16026495,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":35166993,"first_name":"Kofi","last_name":"Aidoo","domain_name":"independent","page_name":"KofiAidoo","display_name":"Kofi Aidoo","profile_url":"https://independent.academia.edu/KofiAidoo?f_ri=1444","photo":"https://gravatar.com/avatar/9f5c8bf6aabe9e4ddf6c2aae1bf7d11b?s=65"}],"research_interests":[{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true},{"id":31632,"name":"Food Microbiology","url":"https://www.academia.edu/Documents/in/Food_Microbiology?f_ri=1444","nofollow":true},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=1444","nofollow":true},{"id":67405,"name":"Toxicity","url":"https://www.academia.edu/Documents/in/Toxicity?f_ri=1444","nofollow":true},{"id":81067,"name":"Bacterial Toxins","url":"https://www.academia.edu/Documents/in/Bacterial_Toxins?f_ri=1444"},{"id":112332,"name":"Bacillus","url":"https://www.academia.edu/Documents/in/Bacillus?f_ri=1444"},{"id":113903,"name":"Bacteria","url":"https://www.academia.edu/Documents/in/Bacteria?f_ri=1444"},{"id":483714,"name":"Bacillus megaterium","url":"https://www.academia.edu/Documents/in/Bacillus_megaterium?f_ri=1444"},{"id":568572,"name":"Bacillus Cereus","url":"https://www.academia.edu/Documents/in/Bacillus_Cereus?f_ri=1444"},{"id":1135814,"name":"Drug Stability","url":"https://www.academia.edu/Documents/in/Drug_Stability?f_ri=1444"},{"id":1181265,"name":"Bacillus licheniformis","url":"https://www.academia.edu/Documents/in/Bacillus_licheniformis?f_ri=1444"},{"id":1491514,"name":"*Hot Temperature","url":"https://www.academia.edu/Documents/in/_Hot_Temperature?f_ri=1444"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_21144103" data-work_id="21144103" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/21144103/Succession_in_a_microbial_mat_community_A_gaian_perspective">Succession in a microbial mat community: A gaian perspective</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">The Gaia hypothesis originally proposed by James E. Lovelock states that the composition, oxidation-reduction state and temperature of the troposphere are actively regulated by the activities of the biota. The gaian concept has been... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_21144103" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">The Gaia hypothesis originally proposed by James E. Lovelock states that the composition, oxidation-reduction state and temperature of the troposphere are actively regulated by the activities of the biota. The gaian concept has been extrapolated to include the composition of surface sediments through the process of biomlneralization. The stratified microbial community dominated by the cyanobacterium Microcoleus chthonopZustes is actively involved in the deposition of laminated sediments at Laguna Figueroa, Baja California, Mexico. Unusually heavy rains in the winters of 1979 and 1980 flooded the evaporite flat with up to 3 meters of meteoric water and deposited 5 -10 cm of allocthonous sediment. The composition of the microbial community changed as a succession of dominating microbial species ensued, ultimately leading to the recolonization of the surface sediment by the original Microcoleusdominated community. The resiliency of bacterial communities is suggested to be an important mechanism of gaian control systems.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/21144103" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="c66d261ad0681fa2272fe925939b6a10" rel="nofollow" data-download="{"attachment_id":41734667,"asset_id":21144103,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/41734667/download_file?st=MTc0MDU3NTMzMyw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="12704540" href="https://duq.academia.edu/JohnStolz">John Stolz</a><script data-card-contents-for-user="12704540" type="text/json">{"id":12704540,"first_name":"John","last_name":"Stolz","domain_name":"duq","page_name":"JohnStolz","display_name":"John Stolz","profile_url":"https://duq.academia.edu/JohnStolz?f_ri=1444","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_21144103 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="21144103"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 21144103, container: ".js-paper-rank-work_21144103", }); 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Lovelock states that the composition, oxidation-reduction state and temperature of the troposphere are actively regulated by the activities of the biota. The gaian concept has been extrapolated to include the composition of surface sediments through the process of biomlneralization. The stratified microbial community dominated by the cyanobacterium Microcoleus chthonopZustes is actively involved in the deposition of laminated sediments at Laguna Figueroa, Baja California, Mexico. Unusually heavy rains in the winters of 1979 and 1980 flooded the evaporite flat with up to 3 meters of meteoric water and deposited 5 -10 cm of allocthonous sediment. The composition of the microbial community changed as a succession of dominating microbial species ensued, ultimately leading to the recolonization of the surface sediment by the original Microcoleusdominated community. The resiliency of bacterial communities is suggested to be an important mechanism of gaian control systems.","downloadable_attachments":[{"id":41734667,"asset_id":21144103,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":12704540,"first_name":"John","last_name":"Stolz","domain_name":"duq","page_name":"JohnStolz","display_name":"John Stolz","profile_url":"https://duq.academia.edu/JohnStolz?f_ri=1444","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":60,"name":"Mechanical Engineering","url":"https://www.academia.edu/Documents/in/Mechanical_Engineering?f_ri=1444","nofollow":true},{"id":88,"name":"Aerospace Engineering","url":"https://www.academia.edu/Documents/in/Aerospace_Engineering?f_ri=1444","nofollow":true},{"id":158,"name":"Marine Biology","url":"https://www.academia.edu/Documents/in/Marine_Biology?f_ri=1444","nofollow":true},{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=1444","nofollow":true},{"id":9533,"name":"Minerals","url":"https://www.academia.edu/Documents/in/Minerals?f_ri=1444"},{"id":9752,"name":"Cyanobacteria","url":"https://www.academia.edu/Documents/in/Cyanobacteria?f_ri=1444"},{"id":22088,"name":"Energy Conversion","url":"https://www.academia.edu/Documents/in/Energy_Conversion?f_ri=1444"},{"id":29487,"name":"Space","url":"https://www.academia.edu/Documents/in/Space?f_ri=1444"},{"id":35399,"name":"Control system","url":"https://www.academia.edu/Documents/in/Control_system?f_ri=1444"},{"id":118671,"name":"Microbial Mat","url":"https://www.academia.edu/Documents/in/Microbial_Mat?f_ri=1444"},{"id":373754,"name":"Ecosystem","url":"https://www.academia.edu/Documents/in/Ecosystem?f_ri=1444"},{"id":646441,"name":"Water Microbiology","url":"https://www.academia.edu/Documents/in/Water_Microbiology?f_ri=1444"},{"id":1256666,"name":"Geologic 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