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style="width: 100%;"><div class="u-taCenter"></div><div class="profile--tab_content_container js-tab-pane tab-pane active" id="all"><div class="profile--tab_heading_container js-section-heading" data-section="Papers" id="Papers"><h3 class="profile--tab_heading_container">Papers by aliaa elborai</h3></div><div class="js-work-strip profile--work_container" data-work-id="90406323"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/90406323/Optimization_Of_Lead_Bioremediation_By_Marine_Halomonas_Sp_Es015_Using_Statistical_Experimental_Methods"><img alt="Research paper thumbnail of Optimization Of Lead Bioremediation By Marine Halomonas Sp. Es015 Using Statistical Experimental Methods" class="work-thumbnail" src="https://attachments.academia-assets.com/93978182/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/90406323/Optimization_Of_Lead_Bioremediation_By_Marine_Halomonas_Sp_Es015_Using_Statistical_Experimental_Methods">Optimization Of Lead Bioremediation By Marine Halomonas Sp. Es015 Using Statistical Experimental Methods</a></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Bioremediation technology is now used for treatment instead of traditional metal removal methods....</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Bioremediation technology is now used for treatment instead of traditional metal removal methods. A strain was isolated from Marsa Alam, Red sea, Egypt showed high resistance to high lead concentration and was identified by the 16S rRNA gene sequencing technique as &lt;em&gt;Halomonas&lt;/em&gt; sp. ES015. Medium optimization was carried out using Plackett-Burman design, and the most significant factors were yeast extract, casamino acid and inoculums size. The optimized media obtained by the statistical design raised the removal efficiency from 84% to 99% from initial concentration 250 ppm of lead. Moreover, Box-Behnken experimental design was applied to study the relationship between yeast extract concentration, casamino acid concentration and inoculums size. The optimized medium increased removal efficiency to 97% from initial concentration 500 ppm of lead. Immobilized &lt;em&gt;Halomonas&lt;/em&gt; sp. ES015 cells on sponge cubes, using optimized medium in loop bioremediation col...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="3bf7827e4e2eb87d7841d9483f3b80a2" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":93978182,"asset_id":90406323,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/93978182/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="90406323"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="90406323"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 90406323; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=90406323]").text(description); $(".js-view-count[data-work-id=90406323]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 90406323; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='90406323']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "3bf7827e4e2eb87d7841d9483f3b80a2" } } $('.js-work-strip[data-work-id=90406323]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":90406323,"title":"Optimization Of Lead Bioremediation By Marine Halomonas Sp. 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The hydrocarbons in cru...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Crude oil is the original mixture of a variety of petroleum hydrocarbons. The hydrocarbons in crude oil are mostly alkanes, cycloalkanes, and various aromatic hydrocarbons, while the other organic compounds contain nitrogen, oxygen, and sulfur, plus trace amounts of metals such as iron, nickel, copper, and vanadium. Polycyclic aromatic hydrocarbons (PAH)-especially the high molecular weight ones (HMWPAH)-are the most dangerous due to their xenobiotic and carcinogenic nature [1].</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="124590b70d54e1d1d4448d543eb4a3c7" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":93978137,"asset_id":90406322,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/93978137/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="90406322"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="90406322"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 90406322; 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="90406321"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/90406321/Optimization_of_medium_composition_for_extra_cellular_alginate_lyases_of_a_marine_bacterium"><img alt="Research paper thumbnail of Optimization of medium composition for extra cellular alginate lyases of a marine bacterium" class="work-thumbnail" src="https://attachments.academia-assets.com/93978181/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/90406321/Optimization_of_medium_composition_for_extra_cellular_alginate_lyases_of_a_marine_bacterium">Optimization of medium composition for extra cellular alginate lyases of a marine bacterium</a></div><div class="wp-workCard_item"><span>African Journal of Microbiology Research</span><span>, 2012</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">In this study, initial minimum inhibitory concentration (MIC) of Pb(II) ions was analysed to chec...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">In this study, initial minimum inhibitory concentration (MIC) of Pb(II) ions was analysed to check optimum concentration of Pb(II) ions at which the growth of sulphate-reducing consortium (SRC) was found to be maximum. 80 ppm of Pb(II) ions was investigated as minimum inhibitory concentration for SRC. Influence of electron donors such as lactose, sucrose, glucose and sodium lactate was examined to investigate best carbon source for growth and activity of sulphate-reducing bacteria. Sodium lactate was found to be the prime carbon source for SRC. Later optimization of various parameters was executed using Box-Behnken design model of response surface methodology to explore the effectiveness of three independent operating variables, namely, pH (5.0-9.0), temperature (32-42掳C) and time (5.0-9.0 days), on dependent variables, i.e. protein content, precipitation of Pb(II) ions, and removal of COD by SRC biomass. Maximum removal of COD and Pb(II) was observed to be 91 and 98 %, respectively, at pH 7.0 and temperature 37掳C and incubation time 7 days. According to response surface analysis and analysis of variance, the experimental data were perfectly fitted to the quadratic model, and the interactive influence of pH, temperature and time on Pb(II) and COD removal was highly significant. A high regression coefficient between the variables and response (r 2 = 0.9974) corroborate eminent evaluation of experimental data by second-order polynomial regression model. SEM and Fourier transform infrared analysis was performed to investigate morphology of PbS precipitates, sorption mechanism and involved functional groups in metal-free and metal-loaded biomass of SRC for Pb(II) binding.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="da7cf77dfde6a0dae8e3ea690d71ab80" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":93978181,"asset_id":90406321,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/93978181/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="90406321"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="90406321"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 90406321; 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> </div><div class="profile--tab_content_container js-tab-pane tab-pane" data-section-id="16327824" id="papers"><div class="js-work-strip profile--work_container" data-work-id="90406323"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/90406323/Optimization_Of_Lead_Bioremediation_By_Marine_Halomonas_Sp_Es015_Using_Statistical_Experimental_Methods"><img alt="Research paper thumbnail of Optimization Of Lead Bioremediation By Marine Halomonas Sp. Es015 Using Statistical Experimental Methods" class="work-thumbnail" src="https://attachments.academia-assets.com/93978182/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/90406323/Optimization_Of_Lead_Bioremediation_By_Marine_Halomonas_Sp_Es015_Using_Statistical_Experimental_Methods">Optimization Of Lead Bioremediation By Marine Halomonas Sp. Es015 Using Statistical Experimental Methods</a></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Bioremediation technology is now used for treatment instead of traditional metal removal methods....</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Bioremediation technology is now used for treatment instead of traditional metal removal methods. A strain was isolated from Marsa Alam, Red sea, Egypt showed high resistance to high lead concentration and was identified by the 16S rRNA gene sequencing technique as &lt;em&gt;Halomonas&lt;/em&gt; sp. ES015. Medium optimization was carried out using Plackett-Burman design, and the most significant factors were yeast extract, casamino acid and inoculums size. The optimized media obtained by the statistical design raised the removal efficiency from 84% to 99% from initial concentration 250 ppm of lead. Moreover, Box-Behnken experimental design was applied to study the relationship between yeast extract concentration, casamino acid concentration and inoculums size. The optimized medium increased removal efficiency to 97% from initial concentration 500 ppm of lead. Immobilized &lt;em&gt;Halomonas&lt;/em&gt; sp. ES015 cells on sponge cubes, using optimized medium in loop bioremediation col...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="3bf7827e4e2eb87d7841d9483f3b80a2" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":93978182,"asset_id":90406323,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/93978182/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="90406323"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="90406323"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 90406323; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=90406323]").text(description); $(".js-view-count[data-work-id=90406323]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 90406323; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='90406323']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "3bf7827e4e2eb87d7841d9483f3b80a2" } } $('.js-work-strip[data-work-id=90406323]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":90406323,"title":"Optimization Of Lead Bioremediation By Marine Halomonas Sp. 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The hydrocarbons in cru...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Crude oil is the original mixture of a variety of petroleum hydrocarbons. The hydrocarbons in crude oil are mostly alkanes, cycloalkanes, and various aromatic hydrocarbons, while the other organic compounds contain nitrogen, oxygen, and sulfur, plus trace amounts of metals such as iron, nickel, copper, and vanadium. Polycyclic aromatic hydrocarbons (PAH)-especially the high molecular weight ones (HMWPAH)-are the most dangerous due to their xenobiotic and carcinogenic nature [1].</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="124590b70d54e1d1d4448d543eb4a3c7" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":93978137,"asset_id":90406322,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/93978137/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="90406322"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="90406322"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 90406322; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=90406322]").text(description); $(".js-view-count[data-work-id=90406322]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 90406322; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='90406322']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="90406321"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/90406321/Optimization_of_medium_composition_for_extra_cellular_alginate_lyases_of_a_marine_bacterium"><img alt="Research paper thumbnail of Optimization of medium composition for extra cellular alginate lyases of a marine bacterium" class="work-thumbnail" src="https://attachments.academia-assets.com/93978181/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/90406321/Optimization_of_medium_composition_for_extra_cellular_alginate_lyases_of_a_marine_bacterium">Optimization of medium composition for extra cellular alginate lyases of a marine bacterium</a></div><div class="wp-workCard_item"><span>African Journal of Microbiology Research</span><span>, 2012</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">In this study, initial minimum inhibitory concentration (MIC) of Pb(II) ions was analysed to chec...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">In this study, initial minimum inhibitory concentration (MIC) of Pb(II) ions was analysed to check optimum concentration of Pb(II) ions at which the growth of sulphate-reducing consortium (SRC) was found to be maximum. 80 ppm of Pb(II) ions was investigated as minimum inhibitory concentration for SRC. Influence of electron donors such as lactose, sucrose, glucose and sodium lactate was examined to investigate best carbon source for growth and activity of sulphate-reducing bacteria. Sodium lactate was found to be the prime carbon source for SRC. Later optimization of various parameters was executed using Box-Behnken design model of response surface methodology to explore the effectiveness of three independent operating variables, namely, pH (5.0-9.0), temperature (32-42掳C) and time (5.0-9.0 days), on dependent variables, i.e. protein content, precipitation of Pb(II) ions, and removal of COD by SRC biomass. Maximum removal of COD and Pb(II) was observed to be 91 and 98 %, respectively, at pH 7.0 and temperature 37掳C and incubation time 7 days. According to response surface analysis and analysis of variance, the experimental data were perfectly fitted to the quadratic model, and the interactive influence of pH, temperature and time on Pb(II) and COD removal was highly significant. A high regression coefficient between the variables and response (r 2 = 0.9974) corroborate eminent evaluation of experimental data by second-order polynomial regression model. SEM and Fourier transform infrared analysis was performed to investigate morphology of PbS precipitates, sorption mechanism and involved functional groups in metal-free and metal-loaded biomass of SRC for Pb(II) binding.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="da7cf77dfde6a0dae8e3ea690d71ab80" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":93978181,"asset_id":90406321,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/93978181/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="90406321"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="90406321"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 90406321; 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