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li{display:table-cell;float:none!important;width:10%;padding:0 15px;border-right:1px solid rgba(0,0,0,0.05)}.mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li:last-child{border-right-width:0}.mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li.hide-border{border-right-width:0}.mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li a{border-bottom-width:0;padding:9px 15px;line-height:120%}.mm-vertical #Top_bar .menu > li > ul.mfn-megamenu a.mfn-megamenu-title{font-weight:700}.rtl .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li:first-child{border-right-width:0}.rtl .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li:last-child{border-right-width:1px}#Header_creative #Top_bar .menu > li > ul.mfn-megamenu{width:980px!important;margin:0}.header-plain:not(.menuo-right) #Header .top_bar_left{width:auto!important}.header-stack.header-center #Top_bar #menu{display:inline-block!important}.header-simple #Top_bar #menu{display:none;height:auto;width:300px;bottom:auto;top:100%;right:1px;position:absolute;margin:0}.header-simple #Header a.responsive-menu-toggle{display:block;right:10px}.header-simple #Top_bar #menu > ul{width:100%;float:left}.header-simple #Top_bar #menu ul li{width:100%;padding-bottom:0;border-right:0;position:relative}.header-simple #Top_bar #menu ul li a{padding:0 20px;margin:0;display:block;height:auto;line-height:normal;border:none}.header-simple #Top_bar #menu ul li a:after{display:none}.header-simple #Top_bar #menu ul li a span{border:none;line-height:44px;display:inline;padding:0}.header-simple #Top_bar #menu ul li.submenu .menu-toggle{display:block;position:absolute;right:0;top:0;width:44px;height:44px;line-height:44px;font-size:30px;font-weight:300;text-align:center;cursor:pointer;color:#444;opacity:0.33}.header-simple #Top_bar #menu ul li.submenu .menu-toggle:after{content:"+"}.header-simple #Top_bar #menu ul li.hover > .menu-toggle:after{content:"-"}.header-simple #Top_bar #menu ul li.hover a{border-bottom:0}.header-simple #Top_bar #menu ul.mfn-megamenu li .menu-toggle{display:none}.header-simple #Top_bar #menu ul li ul{position:relative!important;left:0!important;top:0;padding:0;margin:0!important;width:auto!important;background-image:none}.header-simple #Top_bar #menu ul li ul li{width:100%!important;display:block;padding:0}.header-simple #Top_bar #menu ul li ul li a{padding:0 20px 0 30px}.header-simple #Top_bar #menu ul li ul li a .menu-arrow{display:none}.header-simple #Top_bar #menu ul li ul li a span{padding:0}.header-simple #Top_bar #menu ul li ul li a span:after{display:none!important}.header-simple #Top_bar .menu > li > ul.mfn-megamenu a.mfn-megamenu-title{text-transform:uppercase;font-weight:400}.header-simple #Top_bar .menu > li > ul.mfn-megamenu > li > ul{display:block!important;position:inherit;left:auto;top:auto}.header-simple #Top_bar #menu ul li ul li ul{border-left:0!important;padding:0;top:0}.header-simple #Top_bar #menu ul li ul li ul li a{padding:0 20px 0 40px}.rtl.header-simple #Top_bar #menu{left:1px;right:auto}.rtl.header-simple #Top_bar a.responsive-menu-toggle{left:10px;right:auto}.rtl.header-simple #Top_bar #menu ul li.submenu .menu-toggle{left:0;right:auto}.rtl.header-simple #Top_bar #menu ul li ul{left:auto!important;right:0!important}.rtl.header-simple #Top_bar #menu ul li ul li a{padding:0 30px 0 20px}.rtl.header-simple #Top_bar #menu ul li ul li ul li a{padding:0 40px 0 20px}.menu-highlight #Top_bar .menu > li{margin:0 2px}.menu-highlight:not(.header-creative) #Top_bar .menu > li > a{margin:20px 0;padding:0;-webkit-border-radius:5px;border-radius:5px}.menu-highlight #Top_bar .menu > li > a:after{display:none}.menu-highlight #Top_bar .menu > li > a span:not(.description){line-height:50px}.menu-highlight #Top_bar .menu > li > a span.description{display:none}.menu-highlight.header-stack #Top_bar .menu > li > a{margin:10px 0!important}.menu-highlight.header-stack #Top_bar .menu > li > a span:not(.description){line-height:40px}.menu-highlight.header-transparent #Top_bar .menu > li > a{margin:5px 0}.menu-highlight.header-simple #Top_bar #menu ul li,.menu-highlight.header-creative #Top_bar #menu ul li{margin:0}.menu-highlight.header-simple #Top_bar #menu ul li > a,.menu-highlight.header-creative #Top_bar #menu ul li > a{-webkit-border-radius:0;border-radius:0}.menu-highlight:not(.header-fixed):not(.header-simple) #Top_bar.is-sticky .menu > li > a{margin:10px 0!important;padding:5px 0!important}.menu-highlight:not(.header-fixed):not(.header-simple) #Top_bar.is-sticky .menu > li > a span{line-height:30px!important}.header-modern.menu-highlight.menuo-right .menu_wrapper{margin-right:20px}.menu-line-below #Top_bar .menu > li > a:after{top:auto;bottom:-4px}.menu-line-below #Top_bar.is-sticky .menu > li > a:after{top:auto;bottom:-4px}.menu-line-below-80 #Top_bar:not(.is-sticky) .menu > li > a:after{height:4px;left:10%;top:50%;margin-top:20px;width:80%}.menu-line-below-80-1 #Top_bar:not(.is-sticky) .menu > li > a:after{height:1px;left:10%;top:50%;margin-top:20px;width:80%}.menu-link-color #Top_bar .menu > li > a:after{display:none!important}.menu-arrow-top #Top_bar .menu > li > a:after{background:none repeat scroll 0 0 rgba(0,0,0,0)!important;border-color:#ccc transparent transparent;border-style:solid;border-width:7px 7px 0;display:block;height:0;left:50%;margin-left:-7px;top:0!important;width:0}.menu-arrow-top.header-transparent #Top_bar .menu > li > a:after,.menu-arrow-top.header-plain #Top_bar .menu > li > a:after{display:none}.menu-arrow-top #Top_bar.is-sticky .menu > li > a:after{top:0!important}.menu-arrow-bottom #Top_bar .menu > li > a:after{background:none!important;border-color:transparent transparent #ccc;border-style:solid;border-width:0 7px 7px;display:block;height:0;left:50%;margin-left:-7px;top:auto;bottom:0;width:0}.menu-arrow-bottom.header-transparent #Top_bar .menu > li > a:after,.menu-arrow-bottom.header-plain #Top_bar .menu > li > a:after{display:none}.menu-arrow-bottom #Top_bar.is-sticky .menu > li > a:after{top:auto;bottom:0}.menuo-no-borders #Top_bar .menu > li > a span:not(.description){border-right-width:0}.menuo-no-borders #Header_creative #Top_bar .menu > li > a span{border-bottom-width:0}body.header-creative{padding-left:50px}body.header-creative.header-open{padding-left:250px}body.error404,body.under-construction,body.template-blank{padding-left:0!important}.header-creative.footer-fixed #Footer,.header-creative.footer-sliding #Footer,.header-creative.footer-stick #Footer.is-sticky{box-sizing:border-box;padding-left:50px}.header-open.footer-fixed #Footer,.header-open.footer-sliding #Footer,.header-creative.footer-stick #Footer.is-sticky{padding-left:250px}.header-rtl.header-creative.footer-fixed #Footer,.header-rtl.header-creative.footer-sliding #Footer,.header-rtl.header-creative.footer-stick #Footer.is-sticky{padding-left:0;padding-right:50px}.header-rtl.header-open.footer-fixed #Footer,.header-rtl.header-open.footer-sliding #Footer,.header-rtl.header-creative.footer-stick #Footer.is-sticky{padding-right:250px}#Header_creative{background:#fff;position:fixed;width:250px;height:100%;left:-200px;top:0;z-index:9002;-webkit-box-shadow:2px 0 4px 2px rgba(0,0,0,.15);box-shadow:2px 0 4px 2px rgba(0,0,0,.15)}#Header_creative .container{width:100%}#Header_creative .creative-wrapper{opacity:0;margin-right:50px}#Header_creative a.creative-menu-toggle{display:block;width:34px;height:34px;line-height:34px;font-size:22px;text-align:center;position:absolute;top:10px;right:8px;border-radius:3px}.admin-bar #Header_creative a.creative-menu-toggle{top:42px}#Header_creative #Top_bar{position:static;width:100%}#Header_creative #Top_bar .top_bar_left{width:100%!important;float:none}#Header_creative #Top_bar .top_bar_right{width:100%!important;float:none;height:auto;margin-bottom:35px;text-align:center;padding:0 20px;top:0;-webkit-box-sizing:border-box;-moz-box-sizing:border-box;box-sizing:border-box}#Header_creative #Top_bar .top_bar_right:before{display:none}#Header_creative #Top_bar .top_bar_right_wrapper{top:0}#Header_creative #Top_bar .logo{float:none;text-align:center;margin:15px 0}#Header_creative #Top_bar .menu_wrapper{float:none;margin:0 0 30px}#Header_creative #Top_bar .menu > li{width:100%;float:none;position:relative}#Header_creative #Top_bar .menu > li > a{padding:0;text-align:center}#Header_creative #Top_bar .menu > li > a:after{display:none}#Header_creative #Top_bar .menu > li > a span{border-right:0;border-bottom-width:1px;line-height:38px}#Header_creative #Top_bar .menu li ul{left:100%;right:auto;top:0;box-shadow:2px 2px 2px 0 rgba(0,0,0,0.03);-webkit-box-shadow:2px 2px 2px 0 rgba(0,0,0,0.03)}#Header_creative #Top_bar .menu > li > ul.mfn-megamenu{width:700px!important}#Header_creative #Top_bar .menu > li > ul.mfn-megamenu > li > ul{left:0}#Header_creative #Top_bar .menu li ul li a{padding-top:9px;padding-bottom:8px}#Header_creative #Top_bar .menu li ul li ul{top:0!important}#Header_creative #Top_bar .menu > li > a span.description{display:block;font-size:13px;line-height:28px!important;clear:both}#Header_creative #Top_bar .search_wrapper{left:100%;top:auto;bottom:0}#Header_creative #Top_bar a#header_cart{display:inline-block;float:none;top:3px}#Header_creative #Top_bar a#search_button{display:inline-block;float:none;top:3px}#Header_creative #Top_bar .wpml-languages{display:inline-block;float:none;top:0}#Header_creative #Top_bar .wpml-languages.enabled:hover a.active{padding-bottom:9px}#Header_creative #Top_bar a.button.action_button{display:inline-block;float:none;top:16px;margin:0}#Header_creative #Top_bar .banner_wrapper{display:block;text-align:center}#Header_creative #Top_bar .banner_wrapper img{max-width:100%;height:auto;display:inline-block}#Header_creative #Action_bar{display:none;position:absolute;bottom:0;top:auto;clear:both;padding:0 20px;box-sizing:border-box}#Header_creative #Action_bar .social{float:none;text-align:center;padding:5px 0 15px}#Header_creative #Action_bar .social li{margin-bottom:2px}#Header_creative .social li a{color:rgba(0,0,0,.5)}#Header_creative .social li a:hover{color:#000}#Header_creative .creative-social{position:absolute;bottom:10px;right:0;width:50px}#Header_creative .creative-social li{display:block;float:none;width:100%;text-align:center;margin-bottom:5px}.header-creative .fixed-nav.fixed-nav-prev{margin-left:50px}.header-creative.header-open .fixed-nav.fixed-nav-prev{margin-left:250px}.menuo-last #Header_creative #Top_bar .menu li.last ul{top:auto;bottom:0}.header-open #Header_creative{left:0}.header-open #Header_creative .creative-wrapper{opacity:1;margin:0!important}.header-open #Header_creative .creative-menu-toggle,.header-open #Header_creative .creative-social{display:none}body.header-rtl.header-creative{padding-left:0;padding-right:50px}.header-rtl #Header_creative{left:auto;right:-200px}.header-rtl.nice-scroll #Header_creative{margin-right:10px}.header-rtl #Header_creative .creative-wrapper{margin-left:50px;margin-right:0}.header-rtl #Header_creative a.creative-menu-toggle{left:8px;right:auto}.header-rtl #Header_creative .creative-social{left:0;right:auto}.header-rtl #Footer #back_to_top.sticky{right:125px}.header-rtl #popup_contact{right:70px}.header-rtl #Header_creative #Top_bar .menu li ul{left:auto;right:100%}.header-rtl #Header_creative #Top_bar .search_wrapper{left:auto;right:100%}.header-rtl .fixed-nav.fixed-nav-prev{margin-left:0!important}.header-rtl .fixed-nav.fixed-nav-next{margin-right:50px}body.header-rtl.header-creative.header-open{padding-left:0;padding-right:250px!important}.header-rtl.header-open #Header_creative{left:auto;right:0}.header-rtl.header-open #Footer #back_to_top.sticky{right:325px}.header-rtl.header-open #popup_contact{right:270px}.header-rtl.header-open .fixed-nav.fixed-nav-next{margin-right:250px}#Header_creative.active{left:-1px}.header-rtl #Header_creative.active{left:auto;right:-1px}#Header_creative.active .creative-wrapper{opacity:1;margin:0}.header-creative .vc_row[data-vc-full-width]{padding-left:50px}.header-creative.header-open .vc_row[data-vc-full-width]{padding-left:250px}.header-open .vc_parallax .vc_parallax-inner{left:auto;width:calc(100% - 250px)}.header-open.header-rtl .vc_parallax .vc_parallax-inner{left:0;right:auto}#Header_creative.scroll{height:100%;overflow-y:auto}#Header_creative.scroll:not(.dropdown) .menu li ul{display:none!important}#Header_creative.scroll #Action_bar{position:static}#Header_creative.dropdown{outline:none}#Header_creative.dropdown #Top_bar .menu_wrapper{float:left}#Header_creative.dropdown #Top_bar #menu ul li{position:relative;float:left}#Header_creative.dropdown #Top_bar #menu ul li a:after{display:none}#Header_creative.dropdown #Top_bar #menu ul li a span{line-height:38px;padding:0}#Header_creative.dropdown #Top_bar #menu ul li.submenu .menu-toggle{display:block;position:absolute;right:0;top:0;width:38px;height:38px;line-height:38px;font-size:26px;font-weight:300;text-align:center;cursor:pointer;color:#444;opacity:0.33}#Header_creative.dropdown #Top_bar #menu ul li.submenu .menu-toggle:after{content:"+"}#Header_creative.dropdown #Top_bar #menu ul li.hover > .menu-toggle:after{content:"-"}#Header_creative.dropdown #Top_bar #menu ul li.hover a{border-bottom:0}#Header_creative.dropdown #Top_bar #menu ul.mfn-megamenu li .menu-toggle{display:none}#Header_creative.dropdown #Top_bar #menu ul li ul{position:relative!important;left:0!important;top:0;padding:0;margin-left:0!important;width:auto!important;background-image:none}#Header_creative.dropdown #Top_bar #menu ul li ul li{width:100%!important}#Header_creative.dropdown #Top_bar #menu ul li ul li a{padding:0 10px;text-align:center}#Header_creative.dropdown #Top_bar #menu ul li ul li a .menu-arrow{display:none}#Header_creative.dropdown #Top_bar #menu ul li ul li a span{padding:0}#Header_creative.dropdown #Top_bar #menu ul li ul li a span:after{display:none!important}#Header_creative.dropdown #Top_bar .menu > li > ul.mfn-megamenu a.mfn-megamenu-title{text-transform:uppercase;font-weight:400}#Header_creative.dropdown #Top_bar .menu > li > ul.mfn-megamenu > li > ul{display:block!important;position:inherit;left:auto;top:auto}#Header_creative.dropdown #Top_bar #menu ul li ul li ul{border-left:0!important;padding:0;top:0}#Header_creative{transition:left .5s ease-in-out,right .5s ease-in-out}#Header_creative .creative-wrapper{transition:opacity .5s ease-in-out,margin 0s ease-in-out .5s}#Header_creative.active .creative-wrapper{transition:opacity .5s ease-in-out,margin 0s ease-in-out}}@media only screen and (min-width:1130px){#Top_bar.is-sticky{position:fixed!important;width:100%;left:0;top:-60px;height:60px;z-index:701;background:#fff;opacity:.97;filter:alpha(opacity = 97);-webkit-box-shadow:0 2px 5px 0 rgba(0,0,0,0.1);-moz-box-shadow:0 2px 5px 0 rgba(0,0,0,0.1);box-shadow:0 2px 5px 0 rgba(0,0,0,0.1)}.layout-boxed.header-boxed #Top_bar.is-sticky{max-width:1130px;left:50%;-webkit-transform:translateX(-50%);transform:translateX(-50%)}.layout-boxed.header-boxed.nice-scroll #Top_bar.is-sticky{margin-left:-5px}#Top_bar.is-sticky .top_bar_left,#Top_bar.is-sticky .top_bar_right,#Top_bar.is-sticky .top_bar_right:before{background:none}#Top_bar.is-sticky .top_bar_right{top:-4px;height:auto}#Top_bar.is-sticky .top_bar_right_wrapper{top:15px}.header-plain #Top_bar.is-sticky .top_bar_right_wrapper{top:0}#Top_bar.is-sticky .logo{width:auto;margin:0 30px 0 20px;padding:0}#Top_bar.is-sticky #logo{padding:5px 0!important;height:50px!important;line-height:50px!important}.logo-no-sticky-padding #Top_bar.is-sticky #logo{height:60px!important;line-height:60px!important}#Top_bar.is-sticky #logo img.logo-main{display:none}#Top_bar.is-sticky #logo img.logo-sticky{display:inline;max-height:35px}#Top_bar.is-sticky .menu_wrapper{clear:none}#Top_bar.is-sticky .menu_wrapper .menu > li > a{padding:15px 0}#Top_bar.is-sticky .menu > li > a,#Top_bar.is-sticky .menu > li > a span{line-height:30px}#Top_bar.is-sticky .menu > li > a:after{top:auto;bottom:-4px}#Top_bar.is-sticky .menu > li > a span.description{display:none}#Top_bar.is-sticky .secondary_menu_wrapper,#Top_bar.is-sticky .banner_wrapper{display:none}.header-overlay #Top_bar.is-sticky{display:none}.sticky-dark #Top_bar.is-sticky{background:rgba(0,0,0,.8)}.sticky-dark #Top_bar.is-sticky #menu{background:rgba(0,0,0,.8)}.sticky-dark #Top_bar.is-sticky .menu > li > a{color:#fff}.sticky-dark #Top_bar.is-sticky .top_bar_right a{color:rgba(255,255,255,.5)}.sticky-dark #Top_bar.is-sticky .wpml-languages a.active,.sticky-dark #Top_bar.is-sticky .wpml-languages ul.wpml-lang-dropdown{background:rgba(0,0,0,0.3);border-color:rgba(0,0,0,0.1)}}@media only screen and (min-width:768px) and (max-width:1130px){.header_placeholder{height:0!important}}@media only screen and (max-width:1129px){#Top_bar #menu{display:none;height:auto;width:100%;bottom:auto;top:100%;right:1px;position:absolute;margin:0}#Top_bar a.responsive-menu-toggle{display:block}#Top_bar #menu > ul{width:100%;float:left}#Top_bar #menu ul li{width:100%;padding-bottom:0;border-right:0;position:relative}#Top_bar #menu ul li a{padding:0 25px;margin:0;display:block;height:auto;line-height:normal;border:none}#Top_bar #menu ul li a:after{display:none}#Top_bar #menu ul li a span{border:none;line-height:44px;display:inline;padding:0}#Top_bar #menu ul li a span.description{margin:0 0 0 5px}#Top_bar #menu ul li.submenu .menu-toggle{display:block;position:absolute;right:15px;top:0;width:44px;height:44px;line-height:44px;font-size:30px;font-weight:300;text-align:center;cursor:pointer;color:#444;opacity:0.33}#Top_bar #menu ul li.submenu .menu-toggle:after{content:"+"}#Top_bar #menu ul li.hover > 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class="ubermenu-target-description ubermenu-target-text">Compact 1 to 8 sample batch processing</span></a></li><li id="menu-item-26911" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26911 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-3" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/ml230-focused-ultrasonicator-500656"><span class="ubermenu-target-title ubermenu-target-text">ML230</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">Parallel sample processing, up to 8 samples</span></a></li><li id="menu-item-26912" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26912 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-3" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/e220evolution-focused-ultrasonicator-500429"><span class="ubermenu-target-title ubermenu-target-text">E220<i>evolution</i></span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">High power, 1 to 8 sample batch processing</span></a></li><li id="menu-item-26913" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26913 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-3" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/e220-focused-ultrasonicator-500239"><span class="ubermenu-target-title ubermenu-target-text">E220</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">High power, 1 to 96 sample batch preparation system</span></a></li></ul></li><li id="menu-item-26914" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-has-children ubermenu-item-26914 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2 ubermenu-has-submenu-drop ubermenu-has-submenu-flyout" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/instruments/high-throughput-focused-ultrasonicators"><span class="ubermenu-target-title ubermenu-target-text">High-throughput Focused-ultrasonicators</span><i class='ubermenu-sub-indicator fas fa-angle-down'></i></a><ul class="ubermenu-submenu ubermenu-submenu-id-26914 ubermenu-submenu-type-flyout ubermenu-submenu-drop ubermenu-submenu-align-left_edge_item ubermenu-submenu-retractor-top" ><li class="ubermenu-retractor ubermenu-retractor-mobile"><i class="fas fa-times"></i> Close</li><li id="menu-item-26915" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26915 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-3" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/le220-plus-focused-ultrasonicator-500569"><span class="ubermenu-target-title ubermenu-target-text">LE220-plus</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">High-throughput, 1 to 96 tube capacity</span></a></li><li id="menu-item-26916" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26916 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-3" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/le220r-plus-focused-ultrasonicator-500578"><span class="ubermenu-target-title ubermenu-target-text">LE220R-plus</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">Robotic version of the LE220-plus</span></a></li><li id="menu-item-30065" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-30065 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-3" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="https://www.covaris.com/le220rsc-focused-ultrasonicator"><span class="ubermenu-target-title ubermenu-target-text">LE220Rsc</span></a></li></ul></li><li id="menu-item-26917" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-has-children ubermenu-item-26917 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2 ubermenu-has-submenu-drop ubermenu-has-submenu-flyout" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/instruments/on-deck-automation-focused-ultrasonicators"><span class="ubermenu-target-title ubermenu-target-text">On-Deck Automation Focused-ultrasonicators</span><i class='ubermenu-sub-indicator fas fa-angle-down'></i></a><ul class="ubermenu-submenu ubermenu-submenu-id-26917 ubermenu-submenu-type-flyout ubermenu-submenu-drop ubermenu-submenu-align-left_edge_item ubermenu-submenu-retractor-top" ><li class="ubermenu-retractor ubermenu-retractor-mobile"><i class="fas fa-times"></i> Close</li><li id="menu-item-26918" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26918 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-3" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/r230-focused-ultrasonicator"><span class="ubermenu-target-title ubermenu-target-text">R230</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">On-deck, next generation Focused-ultrasonicator</span></a></li></ul></li><li id="menu-item-26919" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-has-children ubermenu-item-26919 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2 ubermenu-has-submenu-drop ubermenu-has-submenu-flyout" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/instruments/cryoprep"><span class="ubermenu-target-title ubermenu-target-text">cryoPREP<sup>®</sup> Extraction Systems</span><i class='ubermenu-sub-indicator fas fa-angle-down'></i></a><ul class="ubermenu-submenu ubermenu-submenu-id-26919 ubermenu-submenu-type-flyout ubermenu-submenu-drop ubermenu-submenu-align-left_edge_item ubermenu-submenu-retractor-top" ><li class="ubermenu-retractor ubermenu-retractor-mobile"><i class="fas fa-times"></i> Close</li><li id="menu-item-26920" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26920 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-3" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/cp01-cryoprep-manual-dry-pulverizer-500230"><span class="ubermenu-target-title ubermenu-target-text">CP01 Manual Pulverizer</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">For Tissues 15 mg to 2 g</span></a></li><li id="menu-item-26921" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26921 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-3" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/cp02-cryoprep-automated-dry-pulverizer-110v-500001"><span class="ubermenu-target-title ubermenu-target-text">CP02 Automated Dry Pulverizer</span></a></li><li id="menu-item-26922" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26922 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-3" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/cp03-cryoprep-manual-dry-pulverizer-500049"><span class="ubermenu-target-title ubermenu-target-text">CP03 Manual Pulverizer</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">For Tissues 2 to 5 g</span></a></li></ul></li><li id="menu-item-26923" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26923 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/instruments/sonolab-software"><span class="ubermenu-target-title ubermenu-target-text">SonoLab Software</span></a></li></ul></li><li id="menu-item-26927" class="scroll-menu-top ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-has-children ubermenu-item-26927 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-1 ubermenu-has-submenu-drop ubermenu-has-submenu-flyout" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/kits-reagents"><span class="ubermenu-target-title ubermenu-target-text">Kits & Reagents</span><i class='ubermenu-sub-indicator fas fa-angle-down'></i></a><ul class="ubermenu-submenu ubermenu-submenu-id-26927 ubermenu-submenu-type-flyout ubermenu-submenu-drop ubermenu-submenu-align-left_edge_item ubermenu-submenu-retractor-top" ><li class="ubermenu-retractor ubermenu-retractor-mobile"><i class="fas fa-times"></i> Close</li><li id="menu-item-41083" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-41083 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="https://www.covaris.com/truprep-protein/"><span class="ubermenu-target-title ubermenu-target-text">truPREP® Protein Kits</span></a></li><li id="menu-item-35108" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-35108 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="https://www.covaris.com/truxtrac-ffpe-na-auto-96-kits"><span class="ubermenu-target-title ubermenu-target-text">truXTRAC® FFPE SMART Solutions</span></a></li><li id="menu-item-26931" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26931 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/kits-reagents/cryoprep-0"><span class="ubermenu-target-title ubermenu-target-text">cryoPREP Starter Kits</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">cryoPREP Sample Tubes & Holders</span></a></li><li id="menu-item-26933" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26933 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/kits-reagents/nucleic-acids-shearing-kits-and-buffers"><span class="ubermenu-target-title ubermenu-target-text">Nucleic Acids Shearing Kits and Buffers</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">truSHEAR, truPOP, DNA Verification Kits</span></a></li><li id="menu-item-26929" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26929 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/kits-reagents/truxtrac-ffpe-kits"><span class="ubermenu-target-title ubermenu-target-text">Other truXTRAC<sup>®</sup> FFPE Kits</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">FFPE Biomolecule Extraction Kits</span></a></li><li id="menu-item-26934" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26934 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/kits-reagents/truxtrac-other"><span class="ubermenu-target-title ubermenu-target-text">Other truXTRAC<sup>®</sup> Kits</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">cfDNA, DBS, MALDI-TOF</span></a></li><li id="menu-item-26935" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26935 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/kits-reagents/protein-extraction-buffers"><span class="ubermenu-target-title ubermenu-target-text">Protein Extraction Buffers</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">Native, Total Protein, & Detergent-free Buffers</span></a></li><li id="menu-item-26930" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26930 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/kits-reagents/truchip"><span class="ubermenu-target-title ubermenu-target-text">truChIP<sup>®</sup></span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">Chromatin Shearing Kits</span></a></li></ul></li><li id="menu-item-26936" class="scroll-menu-top ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-has-children ubermenu-item-26936 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-1 ubermenu-has-submenu-drop ubermenu-has-submenu-flyout" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/consumables"><span class="ubermenu-target-title ubermenu-target-text">Consumables – Tubes and Vials</span><i class='ubermenu-sub-indicator fas fa-angle-down'></i></a><ul class="ubermenu-submenu ubermenu-submenu-id-26936 ubermenu-submenu-type-flyout ubermenu-submenu-drop ubermenu-submenu-align-left_edge_item ubermenu-submenu-retractor-top" ><li class="ubermenu-retractor ubermenu-retractor-mobile"><i class="fas fa-times"></i> Close</li><li id="menu-item-26938" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26938 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/consumables/afa-tube"><span class="ubermenu-target-title ubermenu-target-text">AFA-TUBE</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">Laser etched polymer vessel for high-throughput workflows</span></a></li><li id="menu-item-26939" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26939 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/consumables/microtube"><span class="ubermenu-target-title ubermenu-target-text">microTUBE</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">Small sample volumes, 130 µl or less</span></a></li><li id="menu-item-26940" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26940 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/consumables/millitube"><span class="ubermenu-target-title ubermenu-target-text">milliTUBE</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">1 ml or 2 ml sample volume. Good for chromatin shearing</span></a></li><li id="menu-item-26942" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26942 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/consumables/other-afa-tubes"><span class="ubermenu-target-title ubermenu-target-text">Other AFA-TUBEs</span></a></li><li id="menu-item-26943" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26943 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/afa-process-vessels/"><span class="ubermenu-target-title ubermenu-target-text">AFA Process Vessels</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">High power, low temperature processing</span></a></li><li id="menu-item-26944" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26944 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/consumables/g-tube"><span class="ubermenu-target-title ubermenu-target-text">g-TUBE</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">DNA shearing fragments 6 to 20 kb</span></a></li></ul></li><li id="menu-item-26945" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-has-children ubermenu-item-26945 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-1 ubermenu-has-submenu-drop ubermenu-has-submenu-flyout" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/cryoprep-consumables"><span class="ubermenu-target-title ubermenu-target-text">cryoPREP Consumables</span><i class='ubermenu-sub-indicator fas fa-angle-down'></i></a><ul class="ubermenu-submenu ubermenu-submenu-id-26945 ubermenu-submenu-type-flyout ubermenu-submenu-drop ubermenu-submenu-align-left_edge_item ubermenu-submenu-retractor-top" ><li class="ubermenu-retractor ubermenu-retractor-mobile"><i class="fas fa-times"></i> Close</li><li id="menu-item-26947" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26947 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/cryoprep-consumables/tissuetube"><span class="ubermenu-target-title ubermenu-target-text">tissueTUBE</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">Tissue processing devices</span></a></li></ul></li><li id="menu-item-26949" class="scroll-menu-top ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-has-children ubermenu-item-26949 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-1 ubermenu-has-submenu-drop ubermenu-has-submenu-flyout" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/accessories"><span class="ubermenu-target-title ubermenu-target-text">Accessories</span><i class='ubermenu-sub-indicator fas fa-angle-down'></i></a><ul class="ubermenu-submenu ubermenu-submenu-id-26949 ubermenu-submenu-type-flyout ubermenu-submenu-drop ubermenu-submenu-align-left_edge_item ubermenu-submenu-retractor-top" ><li class="ubermenu-retractor ubermenu-retractor-mobile"><i class="fas fa-times"></i> Close</li><li id="menu-item-26952" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26952 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/accessories/automation-accessories"><span class="ubermenu-target-title ubermenu-target-text">Automation Accessories</span></a></li><li id="menu-item-41270" class="ubermenu-item ubermenu-item-type-post_type ubermenu-item-object-page ubermenu-item-41270 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="https://www.covaris.com/r230-accessories"><span class="ubermenu-target-title ubermenu-target-text">R230 Accessories</span></a></li><li id="menu-item-26953" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26953 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/accessories/m220-accessories"><span class="ubermenu-target-title ubermenu-target-text">M220 Accessories</span></a></li><li id="menu-item-26954" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26954 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/accessories/me220-accessories"><span class="ubermenu-target-title ubermenu-target-text">ME220 Accessories</span></a></li><li id="menu-item-30718" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-30718 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/accessories/ml230-accessories"><span class="ubermenu-target-title ubermenu-target-text">ML230 Accessories</span></a></li><li id="menu-item-26955" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26955 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/accessories/s220-accessories"><span class="ubermenu-target-title ubermenu-target-text">S220 Accessories</span></a></li><li id="menu-item-26956" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26956 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/accessories/e220-evolution-accessories"><span class="ubermenu-target-title ubermenu-target-text">E220<i>evolution</i> Accessories</span></a></li><li id="menu-item-26957" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26957 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default 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Practices</span></a></li><li id="menu-item-27005" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-27005 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-3" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/application-technical-notes?filter_tag=Dried%20Blood%20Spots"><span class="ubermenu-target-title ubermenu-target-text">Dried Blood Spot (DBS)</span></a></li><li id="menu-item-27008" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-27008 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-3" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/application-technical-notes?filter_tag=Epigenetic%20Workflows"><span class="ubermenu-target-title ubermenu-target-text">Epigenetic Workflows</span></a></li><li id="menu-item-27007" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom 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class="vc_column-inner vc_custom_1584967111220"><div class="wpb_wrapper"> <div class="wpb_text_column wpb_content_element" > <div class="wpb_wrapper"> <p><img src="https://www.covaris.com/wp/wp-content/uploads/2020/03/AdobeStock_70595496-scaled.jpeg" /></p> </div> </div> </div></div></div><div class="wpb_column vc_column_container vc_col-sm-8"><div class="vc_column-inner"><div class="wpb_wrapper"><h4 style="color: #015cb8;text-align: left" class="vc_custom_heading vc_do_custom_heading vc_custom_1656513251434" >Automated Clinical Rapid Whole Exome Sequencing with the Covaris R230 Focused-ultrasonicator</h4><h6 style="color: #020202;text-align: left" class="vc_custom_heading vc_do_custom_heading" >This application note presents a case study with Radboudumc using our next generation of plate-based Focused-ultrasonicator, the R230, and a streamlined approach to high-throughput NGS library preparation.</h6><div class="vc_btn3-container vc_btn3-left vc_custom_1656513352104 vc_do_btn" ><a style="color:#015cb8;" class="vc_general vc_btn3 vc_btn3-size-md vc_btn3-shape-rounded vc_btn3-style-custom" href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/M020151.pdf" title="" target="_blank">READ MORE</a></div></div></div></div></div><div class="vc_row wpb_row vc_inner vc_row-fluid resources_active vc_custom_1589629025682 vc_row-has-fill vc_row-o-equal-height vc_row-flex"><div class="protocol_heading wpb_column vc_column_container vc_col-sm-6"><div class="vc_column-inner vc_custom_1584710703959"><div class="wpb_wrapper"><h6 style="color: #293e53;text-align: left" class="vc_custom_heading vc_do_custom_heading vc_custom_1589874122999" >Application Notes</h6></div></div></div><div class="protocol_filter wpb_column vc_column_container vc_col-sm-6"><div class="vc_column-inner vc_custom_1589629120385"><div class="wpb_wrapper"><p>Sort By</p><select class="covaris_resources_filter"><option selected="selected" value="popularity">Popularity</option><option value="select">Name</option></select> <div class="wpb_text_column wpb_content_element vc_custom_1589629015671" > <div class="wpb_wrapper"> </div> </div> </div></div></div></div> <div class="wpb_raw_code wpb_raw_html wpb_content_element vc_custom_1587802501912" > <div class="wpb_wrapper"> <div class="protocol_filter_by"> <p>Filtered By:</p> <ul></ul> </div> </div> </div> <div class="wpb_text_column wpb_content_element vc_custom_1585832860777" > <div class="wpb_wrapper"> <div class="protocol_item"> <div class="protocol_filter_count"></div> <p>Items</p> </div> </div> </div> <div class="covaris_resource_content"> <li> <h4>DNA Shearing for Next Generation Sequencing (NGS) with the M220 Focused-ultrasonicator</h4> <h6>Application Notes</h6><h5>DNA Shearing for NGS with the ME220 Focused-ultrasonicator. </h5><a href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/M020013.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19125" rel="nofollow" >Download PDF</a> </li> <li> <h4>Lysis and High Molecular Weight DNA Extraction from Eukaryotic and Prokaryotic Cells Powered by AFA-energetics®</h4> <h6>Application Notes</h6><h5>This application note describes an automatable approach to cell lysis and extraction of high molecular weight DNA from microbial samples, which can be sequenced with long-read sequencing technology. </h5><a href="https://www.covaris.com/wp/wp-content/uploads/m020105.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19150" rel="nofollow" >Download PDF</a> </li> <li> <h4>Lysis and Extraction of Biomolecules from Eukaryotic and Prokaryotic Cells Powered by AFA-energetics®</h4> <h6>Application Notes</h6><h5>Covaris developed a novel automatable approach for the extraction of biomolecules from microbial samples for analysis with mass spectrometry technologies using Adaptive Focused Acoustics® technology. </h5><a href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/M020114.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19246" rel="nofollow" >Download PDF</a> </li> <li> <h4>Automated Sample Preparation Combining Adaptive Focused Acoustics® (AFA®) and Single Pot Solid Phase Sample Preparation (SP3)</h4> <h6>Application Notes</h6><h5> </h5><a href="/wp/wp-content/uploads/resources_pdf/M020152.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="32330" rel="nofollow" >Download PDF</a> </li> <li> <h4>Automated Protein Extraction from Clinical Samples with the Covaris ML230 Focused-ultrasonicator</h4> <h6>Application Notes</h6><h5> </h5><a href="/wp/wp-content/uploads/resources_pdf/m020155.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="34167" rel="nofollow" >Download PDF</a> </li> <li> <h4>Hands-off Sample Preparation Workflow for Laser Capture Microdissection (LCM) Samples using Adaptive Focused Acoustics (AFA)</h4> <h6>Application Notes</h6><h5> </h5><a href="/wp/wp-content/uploads/resources_pdf/M020153.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="32322" rel="nofollow" >Download PDF</a> </li> <li> <h4>Active Extraction of Native Proteins from Yeast using Covaris Adaptive Focused Acoustics® (AFA®)</h4> <h6>Application Notes</h6><h5>Native protein extraction reagents were tested for total protein yield and preservation of enzymatic activity using a “passive extraction” or an AFA-based “active extraction” method, both increased. </h5><a href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/M020021.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19129" rel="nofollow" >Download PDF</a> </li> <li> <h4>Automated Clinical Rapid Whole Exome Sequencing with the Covaris R230 Focused-ultrasonicator</h4> <h6>Application Notes</h6><h5><div class="sec_register_right"> <div class="register_form"> <div class="main_form"><strong>Covaris Technical Note with Radboudumc</strong></div> </div> </div> <div class="sec_register_left"> <div class="sec_detail"> <div class="sec_text" data-id="52941"> DNA fragmentation is a critical step in the preparation of good quality libraries for next-generation sequencing (NGS). Covaris Adaptive Focused Acoustics<sup>®</sup> (AFA<sup>®</sup>) technology remains the gold standard in reproducible and robust fragmentation, especially relevant while working with precious clinical samples. </div> </div> </div> </h5><a href="/wp/wp-content/uploads/resources_pdf/M020151.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="32336" rel="nofollow" >Download PDF</a> </li> <li> <h4>AFA-sonication Followed by Modified Protein Aggregation Capture (APAC) Enables Direct, Reproducible and Non-toxic Sample Preparation of FFPE Tissue for Mass Spectrometry-based Proteomics</h4> <h6>Application Notes</h6><h5>The preparation of formalin-fixed and paraffin-embedded (FFPE) tissue for mass spectrometry-based (MS) proteomics relies on efficient removal of paraffin, which commonly involves toxic xylol as gold standard. </h5><a href="https://www.covaris.com/wp/wp-content/uploads/2020/06/M020141.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="27670" rel="nofollow" >Download PDF</a> </li> <li> <h4>Covaris DNA shearing guide for the Agilent SureSelect Target Enrichment System®</h4> <h6>Application Notes</h6><h5>DNA Shearing </h5><a href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/M020060.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19242" rel="nofollow" >Download PDF</a> </li> <li> <h4>Variability in DNA Fragment Size and Distribution Analysis Across Various Fragment Analyzers</h4> <h6>Application Notes</h6><h5>DNA Fragmentation </h5><a href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/M020131.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19156" rel="nofollow" >Download PDF</a> </li> <li> <h4>Optimizing Sample Fixation and Chromatin Shearing for Improved Sensitivity and Reproducibility of Chromatin Immunoprecipitation</h4> <h6>Application Notes</h6><h5>Adaptive Focused Acoustic (AFA) technology provides precise control over mechanical shearing and thermal control during processing to deliver high quality chromatin for sensitive results. </h5><a href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/M020016.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19127" rel="nofollow" >Download PDF</a> </li> <li> <h4>Robust Sample Preparation for Label-free or Tandem Mass Tag (TMT) LC-MS with Adaptive Focused Acoustics® (AFA®)</h4> <h6>Application Notes</h6><h5> </h5><a href="/wp/wp-content/uploads/resources_pdf/M020154.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="32351" rel="nofollow" >Download PDF</a> </li> <li> <h4>Clinical Tumor Tissue Evaluated with Illumina® TruSight™ Oncology 500 (NGS) Assay and Sheared on the Covaris ML230 Focused-ultrasonicator</h4> <h6>Application Notes</h6><h5>Abstract: DNA fragmentation is a critical step in the preparation of high-quality next generation sequencing (NGS) libraries. Covaris Adaptive Focused Acoustics® (AFA®) technology guarantees highly reproducible fragmentation of nucleic acids, which is especially relevant while working with valuable clinical samples for pan-cancer NGS assays such as the Illumina®’s TruSight™ Oncology 500 (TSO 500) panel. This panel was developed jointly by Illumina® and Covaris to provide high quality sequencing results. The DNA fragmentation protocol was previously released for the Covaris E220evolution, LE220-plus, and ME220 Focused-ultrasonicators [1]. In this application note, we present the ML230 and microTUBE-50 protocol to shear DNA to a fragment distribution optimized for the TSO 500 panel. </h5><a href="https://www.covaris.com/wp/wp-content/uploads/m020157.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="34258" rel="nofollow" >Download PDF</a> </li> <li> <h4>Protein Extraction from Yeast: Comparison of the Covaris Adaptive Focused Acoustics®(AFA) Process to Conventional Bead Beating and Probe Sonication</h4> <h6>Application Notes</h6><h5>The efficiency of several mechanical-based lysis and extraction techniques, such as Adaptive Focused Acoustics (AFA), probe sonication, and bead beating from yeast isolates was compared. </h5><a href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/M020003.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19124" rel="nofollow" >Download PDF</a> </li> <li> <h4>truXTRAC® FFPE RNA Extraction and Purification - Quality Metrics for Clinical Applications Powered by Adaptive Focused Acoustics® (AFA®)</h4> <h6>Application Notes</h6><h5>FFPE RNA Extraction </h5><a href="/wp/wp-content/uploads/M020127.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19155" rel="nofollow" >Download PDF</a> </li> <li> <h4>A Streamlined Workflow to Perform ChIP from FFPE Samples</h4> <h6>Application Notes</h6><h5>We demonstrate how AFA® technology combined with truXTRAC® and truChIP® effectively recovers chromatin suitable for studying histone modifications and transcription factor binding interaction events. </h5><a href="/wp/wp-content/uploads/resources_pdf/M020087.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19147" rel="nofollow" >Download PDF</a> </li> <li> <h4>High Quality DNA and RNA Extraction and Purification from FFPE Samples with Covaris truXTRAC® FFPE tNA Ultra Kit – Magnetic Beads</h4> <h6>Application Notes</h6><h5> </h5><a href="/wp/wp-content/uploads/M020142.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="29732" rel="nofollow" >Download PDF</a> </li> <li> <h4>High-Throughput, Low Volume gDNA Extraction from Whole Blood Enabled by Covaris Adaptive Focused Acoustics® (AFA®) and AFA-TUBE® TPX</h4> <h6>Application Notes</h6><h5>Extraction of gDNA from whole blood is the first step in multiple translational research and molecular diagnostics applications, such as next-generation sequencing, multiplex PCR, qPCR, and ddPCR. </h5><a href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/M020116.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19153" rel="nofollow" >Download PDF</a> </li> <li> <h4>Nuclei EXtraction by SONication - NEXSON</h4> <h6>Application Notes</h6><h5>NEXSON </h5><a href="https://www.covaris.com/wp/wp-content/uploads/M020096.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19161" rel="nofollow" >Download PDF</a> </li> <li> <h4>Optimizing a Dual Fixation Protocol to Study Protein Complexes Binding to Chromatin in vivo</h4> <h6>Application Notes</h6><h5>This application note provides shearing profiles, evaluates epitope integrity each shearing time and ChIP-qPCR results obtained using single step formaldehyde fixation and dual crosslinking protocol. </h5><a href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/M020090.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19149" rel="nofollow" >Download PDF</a> </li> <li> <h4>A Robust and Standardized Workflow to Analyze C. albicans Differential Protein Expression using Tandem Mass Tag (TMT)</h4> <h6>Application Notes</h6><h5> </h5><a href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/m020156.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="34165" rel="nofollow" >Download PDF</a> </li> <li> <h4>Nucleic Acid Extraction from Laser-Capture Microdissected (LCM) FFPE Tissues</h4> <h6>Application Notes</h6><h5>LCM </h5><a href="https://www.covaris.com/wp/wp-content/uploads/M020102.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19163" rel="nofollow" >Download PDF</a> </li> <li> <h4>Tumor Tissue Evaluation with the Illumina® TruSight™ Oncology 500 (NGS) Assay using the Covaris R230 Focused-ultrasonicator for Clinical Research Studies</h4> <h6>Application Notes</h6><h5><strong>Abstract:</strong> Next-generation sequencing (NGS) library preparation workflows comprise DNA fragmentation as an essential step. Covaris’ Adaptive Focused Acoustics® (AFA®) Technology enables precise and high-quality data from NGS workflows by ensuring efficient, reliable, and reproducible nucleic acid fragmentation. Efficient DNA shearing is particularly important when working with precious clinical research samples for Comprehensive Cancer Profiling (CCP) using pan-cancer NGS assays such as Illumina’s TruSight Oncology 500 (TSO 500) panel. The DNA fragmentation protocol for the TSO 500 panel was initially released for the Covaris Focused-ultrasonicators ME220, E220evolution, LE220, and ML230 (1,2). In this application note, we present a DNA fragmentation protocol with the R230 Focused-ultrasonicator using 96 microTUBE plates to achieve the optimal fragment size for the TSO 500 panel. A comparative sequencing analysis between samples sheared with the Illumina-validated protocol on the LE220 focused-ultrasonicator and those sheared using the Covaris developed protocol on the R230 showed equivalent or better results for samples sheared on the R230, while decreasing the sample processing time by ~30%. The results in this application note highlight that the automatable R230 enables efficient, high-throughput workflows with Covaris AFA Technology resulting in the robust, reproducible, and confident fragmentation needed for comprehensive pan-cancer panels such as Illumina´s TruSight Oncology 500 assay. </h5><a href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/m020168.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="38638" rel="nofollow" >Download PDF</a> </li> <li> <h4>Streamlined Ultra Low Sample Input and Processing Volume Chromatin Shearing Protocols for Fly Embryos and Mammalian Cell Lines</h4> <h6>Application Notes</h6><h5>These protocols have been optimized for both mammalian cell lines and fly embryos and reliably fragment chromatin in low volumes from down to 10,000 mammalian cells and 5 stage-17 Drosophila embryos. </h5><a href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/M020055.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19140" rel="nofollow" >Download PDF</a> </li> <li> <h4>Optimized Protocol for Robust Chromatin Shearing and Immunoprecipitation of Human Pancreatic Islets using the Covaris® Focused-ultrasonicator</h4> <h6>Application Notes</h6><h5>We demonstrate how the Covaris S220 Focused-ultrasonicator provides highly reproducible chromatin shearing, resulting in improved signal-to-noise ratios and sensitivity for ChIP-seq or ChIP-qPCR. </h5><a href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/M020070.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19144" rel="nofollow" >Download PDF</a> </li> <li> <h4>Simplifying ChIP from Primary Cells and Clinical Samples for High-throughput Epigenetic Applications</h4> <h6>Application Notes</h6><h5>Large-scale analysis of histone marks and transcription factor interactions uses high-quality chromatin, use of validated ChIP-grade antibodies, and optimized reagents to produce meaningful results. </h5><a href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/M020117.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19154" rel="nofollow" >Download PDF</a> </li> <li> <h4>Covaris cryoPREP® with AFA: Enabling High-resolution Diagnostic Metagenomics</h4> <h6>Application Notes</h6><h5>cryoPREP Applications </h5><a href="https://www.covaris.com/wp/wp-content/uploads/M020119.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19166" rel="nofollow" >Download PDF</a> </li> <li> <h4>Shallow Whole Genome Sequencing Workflow – sWGS</h4> <h6>Application Notes</h6><h5>sWGS </h5><a href="/wp/wp-content/uploads/M020099.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19162" rel="nofollow" >Download PDF</a> </li> <li> <h4>Pipetting Best Practices for Covaris 96 microTUBE Plate and 8 microTUBE Strip in Automated Liquid Handlers</h4> <h6>Application Notes</h6><h5>Covaris labware has multi-well formats, like the 96 microTUBE Plate and 8 microTUBE Strip. The hardware and methods described here maximize the robustness of robot pipetting in Covaris consumables. </h5><a href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/M020085.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19244" rel="nofollow" >Download PDF</a> </li> <li> <h4>Chromatin Isolation by RNA Purification - ChIRP</h4> <h6>Application Notes</h6><h5>ChIRP </h5><a href="https://www.covaris.com/wp/wp-content/uploads/M020092.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19158" rel="nofollow" >Download PDF</a> </li> <li> <h4>Shearing DNA with Confidence: A Robust Method for Generating 3 kb DNA</h4> <h6>Application Notes</h6><h5> </h5><a href="/wp/wp-content/uploads/resources_pdf/M020161.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="36663" rel="nofollow" >Download PDF</a> </li> <li> <h4>Preparation of whole body Caenorhabditis elegans extracts for chromatin immunoprecipitation using the Covaris® S220 Focused-ultrasonicator</h4> <h6>Application Notes</h6><h5>This application note provides a sample preparation protocol using the Covaris S220 that can be used by investigators looking to process whole body C. elegans extracts for ChIP-based applications. </h5><a href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/M020069.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19143" rel="nofollow" >Download PDF</a> </li> <li> <h4>Magnetic Bead-based Clean-up Using AFA-energetics®</h4> <h6>Application Notes</h6><h5>When precisely tuned, this stream of Covaris Adaptive Focused Acoustic energy enables DNA shearing precisely and reliably to desired fragment sizes, in addition to gentle contact-free sample mixing. </h5><a href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/M020088.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19245" rel="nofollow" >Download PDF</a> </li> <li> <h4>Evaluation of Next Generation Library Preparation using DNA Extracted from Dried Blood Spots using truXTRAC® DBS DNA kit</h4> <h6>Application Notes</h6><h5>The truXTRAC DBS DNA kit efficiently extracts NGS-grade DNA. AFA enables rehydration and provides cell lysis and mechanical DNA shearing, resulting in NGS library preparation ready DNA. </h5><a href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/M020057.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19141" rel="nofollow" >Download PDF</a> </li> <li> <h4>HiC Chromatin Immunoprecipitation - Hi-ChIP</h4> <h6>Application Notes</h6><h5>Hi-ChIP </h5><a href="https://www.covaris.com/wp/wp-content/uploads/M020097.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19167" rel="nofollow" >Download PDF</a> </li> <li> <h4>Cross-linked Yeast Chromatin Shearing on the Covaris Focused-ultrasonicator</h4> <h6>Application Notes</h6><h5>The Broad Institute developed a cross-linked chromatin shearing protocol for yeast samples that utilizes the Covaris E210 Focused-ultrasonicator, to be used prior to immunoprecipitation. </h5><a href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/M020077.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19146" rel="nofollow" >Download PDF</a> </li> <li> <h4>Cross-linked Yeast Chromatin shearing on the Covaris E210</h4> <h6>Application Notes</h6><h5>Shearing cross-linked yeast samples on the Covaris E210. </h5><a href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/M020040.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19133" rel="nofollow" >Download PDF</a> </li> <li> <h4>Automated NGS Library Construction with Eppendorf epMotion® 5075t/TMX and Covaris® Focused-ultrasonicators</h4> <h6>Application Notes</h6><h5>This guide provides users with a complete set of protocols that encompass all Covaris AFA® instruments for DNA shearing to be readily compatible with the Agilent SureSelect Target Enrichment System. </h5><a href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/M020064.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19243" rel="nofollow" >Download PDF</a> </li> <li> <h4>Affinity-based Enrichment Analyses of DNA Methylation MCIp, MeDIP, hMeDIP</h4> <h6>Application Notes</h6><h5>MeDIP </h5><a href="https://www.covaris.com/wp/wp-content/uploads/M020091.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19157" rel="nofollow" >Download PDF</a> </li> <li> <h4>An Effect of a Shearing Process on the Re-Sequencing of the Arabidopsis thaliana Genome</h4> <h6>Application Notes</h6><h5>Shearing Process of Arabedopis </h5><a href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/M020041.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19176" rel="nofollow" >Download PDF</a> </li> <li> <h4>Single Cell/Nuclei Isolation from Fresh Frozen Tissue</h4> <h6>Application Notes</h6><h5> </h5><a href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/M020143.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="30491" rel="nofollow" >Download PDF</a> </li> <li> <h4>Cytosine Conversion-based, Genome-wide and Targeted DNA Methylation Analysis</h4> <h6>Application Notes</h6><h5>Targeted DNA Methylation Analysis </h5><a href="/wp/wp-content/uploads/resources_pdf/M020167.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="37202" rel="nofollow" >Download PDF</a> </li> <li> <h4>High-resolution Chromosome Conformation Capture: Hi-C</h4> <h6>Application Notes</h6><h5>Hi-C </h5><a href="https://www.covaris.com/wp/wp-content/uploads/M020094.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19159" rel="nofollow" >Download PDF</a> </li> <li> <h4>Measuring Sodium Dodecyl Sulfate Carryover In Protein Hydrolysates Prepared via Protein Aggregation Capture</h4> <h6>Application Notes</h6><h5>The Covaris PAC workflow is a method for extracting and purifying nucleic acids and liquid chromatography mass spectrometry (LC-MS) ready peptide digests from cultured cells and human tissue. The PAC process was originally designed to function as a protein isolation and purification technique that could be performed in a single vessel. The workflow utilizes paramagnetic beads to indiscriminately bind proteins and to enable the removal of harsh chemicals which are often essential for lysis and solubilization. PAC is highly compatible with a wide range of sample inputs as well as detergents, chaotropes, salts, and solvents [3]. Proteolytic cleavage is performed on-bead, and allows for highly efficient and unbiased sample recovery. The Covaris PAC workflow requires minimal liquid transfer; the workflow starting from cell culture and deparaffinized FFPE tissue can be performed in a single plate format and is fully automatable. The extraction process is facilitated through the use of lysis buffers which contain chaotropic agents or detergents like sodium dodecyl sulfate. While PAC is reputed to generate samples with a high degree of purity, carryover of SDS into the final peptide lysate remains a possible concern due to its suppressive effects on analyte ion signal and fouling of equipment during LC-MS [4]. A method was published describing the colorimetric quantitation of SDS in biological samples [5]. The reported approach relies on measuring the interaction of SDS with the extraction efficiency of methylene blue into chloroform. This application note describes the quantitation of SDS carryover in final tryptic digests of the Covaris PAC workflow to determine if they contain any significant degree of contaminating detergent. </h5><a href="https://www.covaris.com/wp/wp-content/uploads/m020169.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="39857" rel="nofollow" >Download PDF</a> </li> <li> <h4>Chromatin Interaction Analysis by Paired-End Tag Sequencing - ChIA-PET</h4> <h6>Application Notes</h6><h5>ChIA-PET </h5><a href="https://www.covaris.com/wp/wp-content/uploads/M020095.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19160" rel="nofollow" >Download PDF</a> </li> <li> <h4>Formaldehyde-Assisted Isolation of Regulatory Elements - FAIRE</h4> <h6>Application Notes</h6><h5>FAIRE </h5><a href="https://www.covaris.com/wp/wp-content/uploads/M020109.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19165" rel="nofollow" >Download PDF</a> </li> <li> <h4>Mechanical Fragmentation of RNA using the Covaris Adaptive Focused Acoustics® (AFA®) Technology for Development of mRNA-based Vaccine and Gene Therapy Platforms</h4> <h6>Application Notes</h6><h5><h5>Mechanical Fragmentation of RNA using the Covaris Adaptive Focused Acoustics® (AFA®) Technology for Development of mRNA-based Vaccine and Gene Therapy Platforms</h5> Introduction The emergence of Next-Generation Sequencing (NGS) technologies has greatly contributed to targeted gene discovery for the generation of new mRNA-based vaccines and therapeutics [1,2]. With the advancement in the world of NGS technologies, RNA sequencing (RNA-Seq) has rapidly become the method of choice for analyzing the transcriptomes of disease states [3], of biological processes, and across a wide range of clinical study designs. Synthetic mRNA has been considered an emerging biotherapeutic agent for decades [4]. However, the outbreak of the COVID-19 pandemic promoted the application of mRNA technologies in development of SARS-CoV-2 vaccines, and there has been a huge increase in interest in the research and development of mRNA-based vaccines [5]. Despite the tremendous improvement in RNA-based NGS technologies, the mRNA-based sequencing methods still present some unique challenges in production, characterization, and quality controls, especially when compared to their traditional protein-based counterparts. One critical challenge is obtaining products that are of homogeneous sequence. In this regard, NGS has proved to be a key tool to probe and address this challenge. This study, conducted in collaboration with GreenLight Biosciences, presents a mechanical mRNA fragmentation methodology that uses Covaris’ Adaptive Focused Acoustics (AFA) Technology to generate the right-sized population pivotal for successful Illumina® library preparation and reproducible sequencing data. </h5><a href="https://www.covaris.com/wp/wp-content/uploads/m020171.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="39615" rel="nofollow" >Download PDF</a> </li> <li> <h4>High-Throughput, Low Volume gDNA Extraction from Whole Blood Enabled by Covaris Adaptive Focused Acoustics® (AFA®) and AFA-TUBE™ TPX</h4> <h6>Application Notes</h6><h5> </h5><a href="https://covaris.com/wp/wp-content/uploads/resources_pdf/M020116.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="36659" rel="nofollow" >Download PDF</a> </li> <li> <h4>High-throughput RNA-Seq of Low Input and Clinically Derived FFPE Samples using Covaris Adaptive Focused Acoustics® (AFA®) Technology</h4> <h6>Application Notes</h6><h5>RNA-Seq data not only reveal gene expression levels but also provide insight into RNA processing patterns and allele-specific expression. However, due to the variable quality and quantity of RNA obtained from FFPE and single-cell samples, the library preparation process often presents challenges and requires significant improvements. To address these challenges, Covaris Adaptive Focused Acoustics (AFA) Technology offers a flexible solution for customers with varying throughput, batch, and automation needs. This technology enables the routine implementation of large-scale RNA sequencing for degraded samples by using a novel method of RNA fragmentation combined with reduced sample volumes for library preparation. </h5><a href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/M020183-High-throughput-RNA-seq-Rev-A.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="41900" rel="nofollow" >Download PDF</a> </li> <li> <h4>Optimization of Insert Sizes for Illumina’s WGS with a Combination of Covaris AFA-based Shearing and Bead-based Size Selection</h4> <h6>Application Notes</h6><h5>In this study, we provide a short technical note on optimization of insert size using Covaris Adaptive Focused Acoustics® (AFA®) technology in conjunction with bead-based size selection for more robust and reproducible library prep to improve overall NGS results. </h5><a href="https://www.covaris.com/wp/wp-content/uploads/M020179.pdf" target="_blank" onclick="post_count_vistors(this)" 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