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data-section="Papers" id="Papers"><h3 class="profile--tab_heading_container">Papers by Julio Carrero</h3></div><div class="js-work-strip profile--work_container" data-work-id="127950220"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/127950220/Characterization_of_Tau95_led_to_the_identification_of_a_four_subunit_TFIIIC_complex_in_trypanosomatid_parasites"><img alt="Research paper thumbnail of Characterization of Tau95 led to the identification of a four-subunit TFIIIC complex in trypanosomatid parasites" class="work-thumbnail" src="https://attachments.academia-assets.com/121605894/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/127950220/Characterization_of_Tau95_led_to_the_identification_of_a_four_subunit_TFIIIC_complex_in_trypanosomatid_parasites">Characterization of Tau95 led to the identification of a four-subunit TFIIIC complex in trypanosomatid parasites</a></div><div class="wp-workCard_item"><span>Applied Microbiology and Biotechnology</span><span>, Jan 9, 2024</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">RNA polymerase III (RNAP III) synthetizes small essential non-coding RNA molecules such as tRNAs ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">RNA polymerase III (RNAP III) synthetizes small essential non-coding RNA molecules such as tRNAs and 5S rRNA. In yeast and vertebrates, RNAP III needs general transcription factors TFIIIA, TFIIIB, and TFIIIC to initiate transcription. TFIIIC, composed of six subunits, binds to internal promoter elements in RNAP III-dependent genes. Limited information is available about RNAP III transcription in the trypanosomatid protozoa Trypanosoma brucei and Leishmania major, which diverged early from the eukaryotic lineage. Analyses of the first published draft of the trypanosomatid genome sequences failed to recognize orthologs of any of the TFIIIC subunits, suggesting that this transcription factor is absent in these parasites. However, a putative TFIIIC subunit was recently annotated in the databases. Here we characterize this subunit in T. brucei and L. major and demonstrate that it corresponds to Tau95. In silico analyses showed that both proteins possess the typical Tau95 sequences: the DNA binding region and the dimerization domain. As anticipated for a transcription factor, Tau95 localized to the nucleus in insect forms of both parasites. Chromatin immunoprecipitation (ChIP) assays demonstrated that Tau95 binds to tRNA and U2 snRNA genes in T. brucei. Remarkably, by performing tandem affinity purifications we identified orthologs of TFIIIC subunits Tau55, Tau131, and Tau138 in T. brucei and L. major. Thus, contrary to what was assumed, trypanosomatid parasites do possess a TFIIIC complex. Other putative interacting partners of Tau95 were identified in T. brucei and L. major. • A four-subunit TFIIIC complex is present in T. brucei and L. major • TbTau95 associates with tRNA and U2 snRNA genes • Putative interacting partners of Tau95 might include some RNAP II regulators</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="bbcd90dbbe60229b758d4595fd2ae867" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":121605894,"asset_id":127950220,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/121605894/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="127950220"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="127950220"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 127950220; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=127950220]").text(description); $(".js-view-count[data-work-id=127950220]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 127950220; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='127950220']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "bbcd90dbbe60229b758d4595fd2ae867" } } $('.js-work-strip[data-work-id=127950220]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":127950220,"title":"Characterization of Tau95 led to the identification of a four-subunit TFIIIC complex in trypanosomatid parasites","translated_title":"","metadata":{"publisher":"Springer Science+Business Media","grobid_abstract":"RNA polymerase III (RNAP III) synthetizes small essential non-coding RNA molecules such as tRNAs and 5S rRNA. 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As anticipated for a transcription factor, Tau95 localized to the nucleus in insect forms of both parasites. Chromatin immunoprecipitation (ChIP) assays demonstrated that Tau95 binds to tRNA and U2 snRNA genes in T. brucei. Remarkably, by performing tandem affinity purifications we identified orthologs of TFIIIC subunits Tau55, Tau131, and Tau138 in T. brucei and L. major. Thus, contrary to what was assumed, trypanosomatid parasites do possess a TFIIIC complex. Other putative interacting partners of Tau95 were identified in T. brucei and L. major. • A four-subunit TFIIIC complex is present in T. brucei and L. major • TbTau95 associates with tRNA and U2 snRNA genes • Putative interacting partners of Tau95 might include some RNAP II regulators","publication_date":{"day":9,"month":1,"year":2024,"errors":{}},"publication_name":"Applied Microbiology and Biotechnology","grobid_abstract_attachment_id":121605893},"translated_abstract":null,"internal_url":"https://www.academia.edu/127950220/Characterization_of_Tau95_led_to_the_identification_of_a_four_subunit_TFIIIC_complex_in_trypanosomatid_parasites","translated_internal_url":"","created_at":"2025-03-01T12:14:11.824-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":36697270,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":121605894,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/121605894/thumbnails/1.jpg","file_name":"s00253-023-12903-8.pdf","download_url":"https://www.academia.edu/attachments/121605894/download_file","bulk_download_file_name":"Characterization_of_Tau95_led_to_the_ide.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/121605894/s00253-023-12903-8-libre.pdf?1740861333=\u0026response-content-disposition=attachment%3B+filename%3DCharacterization_of_Tau95_led_to_the_ide.pdf\u0026Expires=1743230062\u0026Signature=Nj-F4ZnZfOfFuUrgz0Pz9F5HP9cPynQTILsIefhhi9XH8PCs-xTs-hGTdkrg~FBjFC-ViqB5zMKTQiXyG9EWfwMmKTefGIGzBkrRdab1tBZ0OQmt8zF~hiFVIqftOe-dEesv8XVnlZd05uhrt5vYH9fkrAM-4p~28kK4dS6MBc0AtZPLxB5HTiVLXMu4VKLbPmUZoR95YblCaMmNxvdCb4SxmyMN1-sSKlu4NQsi76PA77q03JB4~4dPq3I10OactGRmd3Dy0L8JsGnUbvRm0LzomEbVMWLbr9jKIuZ8IOgwx-KzsB78kLThqNWljkw5J~7d1sAKh8YsL3tX46TwIQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Characterization_of_Tau95_led_to_the_identification_of_a_four_subunit_TFIIIC_complex_in_trypanosomatid_parasites","translated_slug":"","page_count":18,"language":"en","content_type":"Work","summary":"RNA polymerase III (RNAP III) synthetizes small essential non-coding RNA molecules such as tRNAs and 5S rRNA. In yeast and vertebrates, RNAP III needs general transcription factors TFIIIA, TFIIIB, and TFIIIC to initiate transcription. TFIIIC, composed of six subunits, binds to internal promoter elements in RNAP III-dependent genes. Limited information is available about RNAP III transcription in the trypanosomatid protozoa Trypanosoma brucei and Leishmania major, which diverged early from the eukaryotic lineage. Analyses of the first published draft of the trypanosomatid genome sequences failed to recognize orthologs of any of the TFIIIC subunits, suggesting that this transcription factor is absent in these parasites. However, a putative TFIIIC subunit was recently annotated in the databases. Here we characterize this subunit in T. brucei and L. major and demonstrate that it corresponds to Tau95. In silico analyses showed that both proteins possess the typical Tau95 sequences: the DNA binding region and the dimerization domain. As anticipated for a transcription factor, Tau95 localized to the nucleus in insect forms of both parasites. Chromatin immunoprecipitation (ChIP) assays demonstrated that Tau95 binds to tRNA and U2 snRNA genes in T. brucei. Remarkably, by performing tandem affinity purifications we identified orthologs of TFIIIC subunits Tau55, Tau131, and Tau138 in T. brucei and L. major. Thus, contrary to what was assumed, trypanosomatid parasites do possess a TFIIIC complex. Other putative interacting partners of Tau95 were identified in T. brucei and L. major. • A four-subunit TFIIIC complex is present in T. brucei and L. major • TbTau95 associates with tRNA and U2 snRNA genes • Putative interacting partners of Tau95 might include some RNAP II regulators","owner":{"id":36697270,"first_name":"Julio","middle_initials":null,"last_name":"Carrero","page_name":"JulioCarrero1","domain_name":"independent","created_at":"2015-10-21T12:09:01.815-07:00","display_name":"Julio Carrero","url":"https://independent.academia.edu/JulioCarrero1"},"attachments":[{"id":121605894,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/121605894/thumbnails/1.jpg","file_name":"s00253-023-12903-8.pdf","download_url":"https://www.academia.edu/attachments/121605894/download_file","bulk_download_file_name":"Characterization_of_Tau95_led_to_the_ide.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/121605894/s00253-023-12903-8-libre.pdf?1740861333=\u0026response-content-disposition=attachment%3B+filename%3DCharacterization_of_Tau95_led_to_the_ide.pdf\u0026Expires=1743230062\u0026Signature=Nj-F4ZnZfOfFuUrgz0Pz9F5HP9cPynQTILsIefhhi9XH8PCs-xTs-hGTdkrg~FBjFC-ViqB5zMKTQiXyG9EWfwMmKTefGIGzBkrRdab1tBZ0OQmt8zF~hiFVIqftOe-dEesv8XVnlZd05uhrt5vYH9fkrAM-4p~28kK4dS6MBc0AtZPLxB5HTiVLXMu4VKLbPmUZoR95YblCaMmNxvdCb4SxmyMN1-sSKlu4NQsi76PA77q03JB4~4dPq3I10OactGRmd3Dy0L8JsGnUbvRm0LzomEbVMWLbr9jKIuZ8IOgwx-KzsB78kLThqNWljkw5J~7d1sAKh8YsL3tX46TwIQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"},{"id":121605893,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/121605893/thumbnails/1.jpg","file_name":"s00253-023-12903-8.pdf","download_url":"https://www.academia.edu/attachments/121605893/download_file","bulk_download_file_name":"Characterization_of_Tau95_led_to_the_ide.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/121605893/s00253-023-12903-8-libre.pdf?1740861339=\u0026response-content-disposition=attachment%3B+filename%3DCharacterization_of_Tau95_led_to_the_ide.pdf\u0026Expires=1743230062\u0026Signature=UnbZws9wiY10n7c9jVMqyxJXsykB-pww99xevRsx0dbQVcg5TQC-h9VDuqzmrVZBXvQbiFelKzWTFZDhRcfM2poQTXD3JaszctHZ7NA698O0XsAoD8R0SNAJEhNgqh3bbLNnm2VTqRMEPMKGGPk3sQORq5JK1nKjDQ7-W08DYl-RrLnYQ2IV-AeMj1pYls34llaW-YJuHouXB0dD6S8YJ~YOxO0pRr24spU3ixi06vNlbhRl4ywVFf0OnifeL9f3HGa4FTfqPViw~Zm6AxXsHyQodkHVZ4R~oV87CWOSpfMq7ZnTG9IjtsBZDD8-XMoV-MpXRwO8jIZFI3DddXuZqw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":156,"name":"Genetics","url":"https://www.academia.edu/Documents/in/Genetics"},{"id":803,"name":"Philosophy","url":"https://www.academia.edu/Documents/in/Philosophy"},{"id":3701,"name":"RNA","url":"https://www.academia.edu/Documents/in/RNA"},{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":13827,"name":"Cell Biology","url":"https://www.academia.edu/Documents/in/Cell_Biology"},{"id":15674,"name":"Linguistics","url":"https://www.academia.edu/Documents/in/Linguistics"},{"id":20283,"name":"RNA polymerase","url":"https://www.academia.edu/Documents/in/RNA_polymerase"},{"id":27784,"name":"Gene expression","url":"https://www.academia.edu/Documents/in/Gene_expression"},{"id":121673,"name":"Promoter","url":"https://www.academia.edu/Documents/in/Promoter"},{"id":181936,"name":"Gene","url":"https://www.academia.edu/Documents/in/Gene"},{"id":213901,"name":"Transcription Factor","url":"https://www.academia.edu/Documents/in/Transcription_Factor"},{"id":238813,"name":"Chromatin","url":"https://www.academia.edu/Documents/in/Chromatin"},{"id":269171,"name":"Applied Microbiology and Biotechnology","url":"https://www.academia.edu/Documents/in/Applied_Microbiology_and_Biotechnology"},{"id":389130,"name":"Trypanosoma brucei","url":"https://www.academia.edu/Documents/in/Trypanosoma_brucei"},{"id":440820,"name":"Chromatin Immunoprecipitation","url":"https://www.academia.edu/Documents/in/Chromatin_Immunoprecipitation"},{"id":865697,"name":"In Silico","url":"https://www.academia.edu/Documents/in/In_Silico"},{"id":4181301,"name":"Transfer-RNA","url":"https://www.academia.edu/Documents/in/Transfer_RNA"}],"urls":[{"id":47023489,"url":"https://link.springer.com/content/pdf/10.1007/s00253-023-12903-8.pdf"}]}, dispatcherData: dispatcherData }); 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Lactoferricins (Lfcins) are peptides derived from the N-terminus of Lf. Lf avoids the iron availability to parasites in the body fluids due to its high avidity for iron, maintaining together with transferrin the free-iron concentration in about 10 −18 M, which is too low to support the pathogenic invader survival. Intestinal parasitic diseases affect people worldwide, mainly in developing countries with poor hygienic conditions; for example, parasites such as Entamoeba histolytica, Giardia intestinalis, and Cryptosporidium parvum infect the human intestine when are orally ingested as cysts. Human and bovine Lf have been found parasiticidal in experiments in vitro and in animal models. Interestingly, Lf synergizes with metronidazole, the main drug used against E. histolytica and G. intestinalis. The aim of this chapter is to show the benefits of using Lf and Lfcins against intestinal parasitic diseases.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="5c0a9bf2e287182b8221aff73600919c" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":110480514,"asset_id":113549622,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/110480514/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="113549622"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="113549622"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 113549622; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=113549622]").text(description); $(".js-view-count[data-work-id=113549622]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 113549622; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='113549622']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "5c0a9bf2e287182b8221aff73600919c" } } $('.js-work-strip[data-work-id=113549622]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":113549622,"title":"Lactoferrin in the battle against intestinal parasites","translated_title":"","metadata":{"ai_title_tag":"Lactoferrin's Role Against Intestinal Parasites","grobid_abstract":"Lactoferrin is an iron-binding glycoprotein of the innate immune system, which is present in some mammalian fluids and secreted into the mucosae; it is also produced by the secondary granules of the polymorphonuclear neutrophils and secreted at infection sites. 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Lactoferricins (Lfcins) are peptides derived from the N-terminus of Lf. Lf avoids the iron availability to parasites in the body fluids due to its high avidity for iron, maintaining together with transferrin the free-iron concentration in about 10 −18 M, which is too low to support the pathogenic invader survival. Intestinal parasitic diseases affect people worldwide, mainly in developing countries with poor hygienic conditions; for example, parasites such as Entamoeba histolytica, Giardia intestinalis, and Cryptosporidium parvum infect the human intestine when are orally ingested as cysts. Human and bovine Lf have been found parasiticidal in experiments in vitro and in animal models. Interestingly, Lf synergizes with metronidazole, the main drug used against E. histolytica and G. intestinalis. The aim of this chapter is to show the benefits of using Lf and Lfcins against intestinal parasitic diseases.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="c7a3d544d890578d87c9806b6fe36604" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":110480516,"asset_id":113549621,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/110480516/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="113549621"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="113549621"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 113549621; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=113549621]").text(description); $(".js-view-count[data-work-id=113549621]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 113549621; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='113549621']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "c7a3d544d890578d87c9806b6fe36604" } } $('.js-work-strip[data-work-id=113549621]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":113549621,"title":"Lactoferrin in the Battle against Intestinal Parasites: A Review","translated_title":"","metadata":{"publisher":"InTech","grobid_abstract":"Lactoferrin is an iron-binding glycoprotein of the innate immune system, which is present in some mammalian fluids and secreted into the mucosae; it is also produced by the secondary granules of the polymorphonuclear neutrophils and secreted at infection sites. Lactoferricins (Lfcins) are peptides derived from the N-terminus of Lf. Lf avoids the iron availability to parasites in the body fluids due to its high avidity for iron, maintaining together with transferrin the free-iron concentration in about 10 −18 M, which is too low to support the pathogenic invader survival. Intestinal parasitic diseases affect people worldwide, mainly in developing countries with poor hygienic conditions; for example, parasites such as Entamoeba histolytica, Giardia intestinalis, and Cryptosporidium parvum infect the human intestine when are orally ingested as cysts. Human and bovine Lf have been found parasiticidal in experiments in vitro and in animal models. Interestingly, Lf synergizes with metronidazole, the main drug used against E. histolytica and G. intestinalis. The aim of this chapter is to show the benefits of using Lf and Lfcins against intestinal parasitic diseases.","publication_date":{"day":12,"month":7,"year":2017,"errors":{}},"publication_name":"InTech eBooks","grobid_abstract_attachment_id":110480516},"translated_abstract":null,"internal_url":"https://www.academia.edu/113549621/Lactoferrin_in_the_Battle_against_Intestinal_Parasites_A_Review","translated_internal_url":"","created_at":"2024-01-15T12:51:34.370-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":36697270,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":110480516,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/110480516/thumbnails/1.jpg","file_name":"53563.pdf","download_url":"https://www.academia.edu/attachments/110480516/download_file","bulk_download_file_name":"Lactoferrin_in_the_Battle_against_Intest.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/110480516/53563-libre.pdf?1705355553=\u0026response-content-disposition=attachment%3B+filename%3DLactoferrin_in_the_Battle_against_Intest.pdf\u0026Expires=1743230062\u0026Signature=bohsgEXNf3mZioQzIbe9ga~YGhsjTCxAqp9aRxmZ1mKThebe68vuoRbaLrXz4eoWFcInQ1J1EvRcvJu9aw7W9-n6Yxq8dSrI~BLWsfuvCoqaNij6Bf1O59XaQH7Sk6oCL98faGI6w36LOBxmxSiEUbDWbYTDp5-lkLpG6XkeQnD4PojGRDFQ-lSFKvCGBNa9TXb8F5US0G2gwItVyUcu-NWN0GNDSY65CGDphWffLYSwc5yQQO6wruYkNpp3YPgBfdqyd0brGs4inoEcrHqiB6m8JFQfp8g6vRn87vDp1EPS8qvetf18Djgr6QmsjYsNpxSU~6af5uKJZwa2ERWR0w__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Lactoferrin_in_the_Battle_against_Intestinal_Parasites_A_Review","translated_slug":"","page_count":29,"language":"en","content_type":"Work","summary":"Lactoferrin is an iron-binding glycoprotein of the innate immune system, which is present in some mammalian fluids and secreted into the mucosae; it is also produced by the secondary granules of the polymorphonuclear neutrophils and secreted at infection sites. Lactoferricins (Lfcins) are peptides derived from the N-terminus of Lf. Lf avoids the iron availability to parasites in the body fluids due to its high avidity for iron, maintaining together with transferrin the free-iron concentration in about 10 −18 M, which is too low to support the pathogenic invader survival. Intestinal parasitic diseases affect people worldwide, mainly in developing countries with poor hygienic conditions; for example, parasites such as Entamoeba histolytica, Giardia intestinalis, and Cryptosporidium parvum infect the human intestine when are orally ingested as cysts. Human and bovine Lf have been found parasiticidal in experiments in vitro and in animal models. Interestingly, Lf synergizes with metronidazole, the main drug used against E. histolytica and G. intestinalis. The aim of this chapter is to show the benefits of using Lf and Lfcins against intestinal parasitic diseases.","owner":{"id":36697270,"first_name":"Julio","middle_initials":null,"last_name":"Carrero","page_name":"JulioCarrero1","domain_name":"independent","created_at":"2015-10-21T12:09:01.815-07:00","display_name":"Julio Carrero","url":"https://independent.academia.edu/JulioCarrero1"},"attachments":[{"id":110480516,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/110480516/thumbnails/1.jpg","file_name":"53563.pdf","download_url":"https://www.academia.edu/attachments/110480516/download_file","bulk_download_file_name":"Lactoferrin_in_the_Battle_against_Intest.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/110480516/53563-libre.pdf?1705355553=\u0026response-content-disposition=attachment%3B+filename%3DLactoferrin_in_the_Battle_against_Intest.pdf\u0026Expires=1743230062\u0026Signature=bohsgEXNf3mZioQzIbe9ga~YGhsjTCxAqp9aRxmZ1mKThebe68vuoRbaLrXz4eoWFcInQ1J1EvRcvJu9aw7W9-n6Yxq8dSrI~BLWsfuvCoqaNij6Bf1O59XaQH7Sk6oCL98faGI6w36LOBxmxSiEUbDWbYTDp5-lkLpG6XkeQnD4PojGRDFQ-lSFKvCGBNa9TXb8F5US0G2gwItVyUcu-NWN0GNDSY65CGDphWffLYSwc5yQQO6wruYkNpp3YPgBfdqyd0brGs4inoEcrHqiB6m8JFQfp8g6vRn87vDp1EPS8qvetf18Djgr6QmsjYsNpxSU~6af5uKJZwa2ERWR0w__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology"},{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":132887,"name":"Transferrin","url":"https://www.academia.edu/Documents/in/Transferrin"},{"id":571342,"name":"Entamoeba histolytica","url":"https://www.academia.edu/Documents/in/Entamoeba_histolytica"},{"id":1476273,"name":"Giardia","url":"https://www.academia.edu/Documents/in/Giardia"},{"id":1938317,"name":"Innate Immune System","url":"https://www.academia.edu/Documents/in/Innate_Immune_System"},{"id":2558223,"name":"Cryptosporidium parvum","url":"https://www.academia.edu/Documents/in/Cryptosporidium_parvum"},{"id":2636453,"name":"Lactoferrin","url":"https://www.academia.edu/Documents/in/Lactoferrin"},{"id":3042534,"name":"battle","url":"https://www.academia.edu/Documents/in/battle"}],"urls":[{"id":38604497,"url":"https://doi.org/10.5772/66819"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") if (false) { Aedu.setUpFigureCarousel('profile-work-113549621-figures'); } }); </script> <div class="js-work-strip profile--work_container" data-work-id="113549620"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/113549620/Molecular_characterization_functional_expression_tissue_localization_and_protective_potential_of_a_Taenia_solium_fatty_acid_binding_protein"><img alt="Research paper thumbnail of Molecular characterization, functional expression, tissue localization and protective potential of a Taenia solium fatty acid-binding protein" class="work-thumbnail" src="https://attachments.academia-assets.com/110480513/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/113549620/Molecular_characterization_functional_expression_tissue_localization_and_protective_potential_of_a_Taenia_solium_fatty_acid_binding_protein">Molecular characterization, functional expression, tissue localization and protective potential of a Taenia solium fatty acid-binding protein</a></div><div class="wp-workCard_item"><span>Molecular and Biochemical Parasitology</span><span>, Dec 1, 2012</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The fatty acid-binding proteins (FABPs) comprise a family of proteins that are widely expressed i...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The fatty acid-binding proteins (FABPs) comprise a family of proteins that are widely expressed in animal cells and perform a variety of vital functions. Here, we report the identification, characterization, recombinant expression, tissue localization and protective potential of a Taenia solium FABP (TsFABP1). The TsFABP1 primary structure showed all the conserved residues characteristic of the subfamily iv of the intracellular Lipid-Binding Proteins (iLBPs), including those involved in the binding stabilization of the fatty acid molecule. Through a competitive binding assay we found that TsFABP1 is able to bind at least six different fatty acids with preference toward palmitic and stearic acid, suggesting that TsFABP1 is a member of the iLBP subfamily iv. Immunolocalization assays carried out on larval and adult tissues of four species of taeniids using anti-TsFABP1 hyperimmune sera produced in mice and rabbit, showed intense labeling in the tegument of the spiral canal and in subtegumental cytons of the larvae. These findings suggest that the spiral canal might be a major place for FA uptake in the developing scolex. In contrast, only subtegumental cytons in the adult worms stained positive. We propose that TsFABP1 is involved in the mechanism to mobilize fatty acids between compartments in the extensive syncytial tissue of taeniids. Protection assays carried out in a murine model of cysticercosis showed that subcutaneous immunization with TsFABP1 resulted in about 45% reduction of parasite load against an intraperitoneal challenge with Taenia crassiceps cysts. This reduction in parasite load correlated with the level of cellular and humoral immune responses against TsFABP1, as determined in spleen lymphocyte proliferation and ELISA testing.</span></div><div class="wp-workCard_item"><div class="carousel-container carousel-container--sm" id="profile-work-113549620-figures"><div class="prev-slide-container js-prev-button-container"><button aria-label="Previous" class="carousel-navigation-button js-profile-work-113549620-figures-prev"><span class="material-symbols-outlined" style="font-size: 24px" translate="no">arrow_back_ios</span></button></div><div class="slides-container js-slides-container"><figure class="figure-slide-container"><a href="https://www.academia.edu/figures/53628430/figure-2-recombinant-expression-and-immune-recognition-of"><img alt="Fig. 2. Recombinant expression and immune recognition of TsFABP1.Lanes (A-C): SDS-PAGE of (A) molecular weight markers, (B) crude extract of BL21PLys trans- formed cells and (C) purified TsFABP1 after nickel affinity chromatography; lanes D and E: Western blots of (D) crude antigenic extract (15 wg) of T. solium cysticerci reacted with a hyperimmune anti-TsFABP1 mice serum and (E) crude antigenic extract (15 wg) of T. crassiceps cysticerci reacted against a hyperimmune anti- TsFABP1 rabbit serum. Horseradish peroxidase-goat anti-mice IgG or anti-rabbit IgG were used as second antibodies. 4-Chloro-1-Naphtol was used as developer. " class="figure-slide-image" src="https://figures.academia-assets.com/110480513/figure_001.jpg" /></a></figure><figure class="figure-slide-container"><a href="https://www.academia.edu/figures/53628442/figure-4-humoral-immune-response-of-mice-after-immunization"><img alt="Fig. 4. Humoral immune response of mice after immunization with TsFABP1. Mice were immunized with 50 or 100 wg of TsFABP1, and bled at days 0, 14 and 28. At day 42, mice were challenged by intraperitoneal injection of 10 T. crassiceps cysts and sacrificed at day 94. Results from three independent experiments. " class="figure-slide-image" src="https://figures.academia-assets.com/110480513/figure_002.jpg" /></a></figure><figure class="figure-slide-container"><a href="https://www.academia.edu/figures/53628454/figure-3-tissue-immunolocalization-of-tsfabp-tissue-sections"><img alt="Fig. 3. Tissue immunolocalization of TsFABP1. Tissue sections from cysticerci of T. solium (A, D), T. pisiformis (B, E), T. crassiceps ORF (C, F) and WFU (G, J) strains, and adult worms of T. saginata (H, K) and T. solium (1, L). In A, B, C, G, H and I, the tissue sections were reacted with anti-TsFABP1 hyperimmune sera, whereas in D, E, F, J, K and L, were treated with preimmune sera. The corresponding second antibody was conjugated to horseradish peroxidase. Abbreviations: subtegumental cytons (sc), tegument (tg), bladder wall (bw), parenchyma (pa) and eggs (eg) are indicated by arrows. Reactions were developed with diamino benzidine. All sections were counterstained with hematoxylin and eosin. " class="figure-slide-image" src="https://figures.academia-assets.com/110480513/figure_003.jpg" /></a></figure><figure class="figure-slide-container"><a href="https://www.academia.edu/figures/53628462/figure-5-proliferation-assays-of-mice-spleen-lymphocytes"><img alt="Fig. 5. Proliferation assays of mice spleen lymphocytes after immunization with TsFABP1. Groups of mice were injected with 50 wg of TsFABP1 (immunized) or adju- vant alone (Adjuvant). One group of Adjuvant and one group of immunized mice were challenged with 10 cysts at day 42 and sacrificed at day 94. The other two groups were just sacrificed at day 42. Spleen lymphocytes were obtained after sac- rifice and stimulated with TsFABP1 or with a T. crassiceps crude antigenic extract. No significant differences were found when comparing groups 100 wg vs TSFABP1) (p>0.05). " class="figure-slide-image" src="https://figures.academia-assets.com/110480513/figure_004.jpg" /></a></figure><figure class="figure-slide-container"><a href="https://www.academia.edu/figures/53628470/table-1-tsfabp-binding-affinities-ka-values-in-nm-reported"><img alt="TsFABP1 binding affinities. 4 Ka values in nM, reported as an average of 3 experiments + SD. Table 1 " class="figure-slide-image" src="https://figures.academia-assets.com/110480513/table_001.jpg" /></a></figure><figure class="figure-slide-container"><a href="https://www.academia.edu/figures/53628482/figure-1-molecular-characterization-of-tsfabp-gene-structure"><img alt="Fig. 1. Molecular characterization of TsFABP1. (A) Gene structure of Tsfabp1. The 402 bp coding sequence in mRNA is translated into the 133 amino acid residues (~15 KDa) product; (B) Alignment of selected FABPs amino acid sequences. Abbreviations (GenBank accession numbers): T. solium TsFABP1 (JQ929049), TsFABP2 (JX470484), E. gra- nulosus EgFABP1 (AAK12096) and EgFABP2 (AAK12094), F. gigantica FgFABP (AAD23998), F. hepatica FASHE3 (Q9U1G6), S. mansoni Sm14 (AAT39384), S. japonicum SjFABPc (AAA64425), human FABP8 (NP_002668). In the sequence alignment, identities with TsFABP1 are shown as blank and gaps are shown as dashes. Residues involved in the stabilization of the ligand carboxyl and hydrophobic termini, are shown in dark and light gray, respectively. " class="figure-slide-image" src="https://figures.academia-assets.com/110480513/table_002.jpg" /></a></figure><figure class="figure-slide-container"><a href="https://www.academia.edu/figures/53628489/table-2-was-determined-for-the-immunized-mice-by-comparing"><img alt="* p was determined for the immunized mice by comparing with the adjuvant-only group.’ Results from 3 independent experiments. Protection studies in the murine model of T. crassiceps. Table 2 " class="figure-slide-image" src="https://figures.academia-assets.com/110480513/table_003.jpg" /></a></figure></div><div class="next-slide-container js-next-button-container"><button aria-label="Next" class="carousel-navigation-button js-profile-work-113549620-figures-next"><span class="material-symbols-outlined" style="font-size: 24px" translate="no">arrow_forward_ios</span></button></div></div></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="7d3a5a5913c9ac2503db030700f21584" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":110480513,"asset_id":113549620,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/110480513/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="113549620"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="113549620"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 113549620; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=113549620]").text(description); $(".js-view-count[data-work-id=113549620]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 113549620; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='113549620']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "7d3a5a5913c9ac2503db030700f21584" } } $('.js-work-strip[data-work-id=113549620]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":113549620,"title":"Molecular characterization, functional expression, tissue localization and protective potential of a Taenia solium fatty acid-binding protein","translated_title":"","metadata":{"publisher":"Elsevier BV","grobid_abstract":"The fatty acid-binding proteins (FABPs) comprise a family of proteins that are widely expressed in animal cells and perform a variety of vital functions. Here, we report the identification, characterization, recombinant expression, tissue localization and protective potential of a Taenia solium FABP (TsFABP1). The TsFABP1 primary structure showed all the conserved residues characteristic of the subfamily iv of the intracellular Lipid-Binding Proteins (iLBPs), including those involved in the binding stabilization of the fatty acid molecule. Through a competitive binding assay we found that TsFABP1 is able to bind at least six different fatty acids with preference toward palmitic and stearic acid, suggesting that TsFABP1 is a member of the iLBP subfamily iv. Immunolocalization assays carried out on larval and adult tissues of four species of taeniids using anti-TsFABP1 hyperimmune sera produced in mice and rabbit, showed intense labeling in the tegument of the spiral canal and in subtegumental cytons of the larvae. These findings suggest that the spiral canal might be a major place for FA uptake in the developing scolex. In contrast, only subtegumental cytons in the adult worms stained positive. We propose that TsFABP1 is involved in the mechanism to mobilize fatty acids between compartments in the extensive syncytial tissue of taeniids. Protection assays carried out in a murine model of cysticercosis showed that subcutaneous immunization with TsFABP1 resulted in about 45% reduction of parasite load against an intraperitoneal challenge with Taenia crassiceps cysts. This reduction in parasite load correlated with the level of cellular and humoral immune responses against TsFABP1, as determined in spleen lymphocyte proliferation and ELISA testing.","publication_date":{"day":1,"month":12,"year":2012,"errors":{}},"publication_name":"Molecular and Biochemical Parasitology","grobid_abstract_attachment_id":110480513},"translated_abstract":null,"internal_url":"https://www.academia.edu/113549620/Molecular_characterization_functional_expression_tissue_localization_and_protective_potential_of_a_Taenia_solium_fatty_acid_binding_protein","translated_internal_url":"","created_at":"2024-01-15T12:51:34.177-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":36697270,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":110480513,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/110480513/thumbnails/1.jpg","file_name":"j.molbiopara.2012.10.00220240115-1-3gb6fm.pdf","download_url":"https://www.academia.edu/attachments/110480513/download_file","bulk_download_file_name":"Molecular_characterization_functional_ex.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/110480513/j.molbiopara.2012.10.00220240115-1-3gb6fm-libre.pdf?1705355553=\u0026response-content-disposition=attachment%3B+filename%3DMolecular_characterization_functional_ex.pdf\u0026Expires=1743230062\u0026Signature=dScZ~48P6TirXJwkm4~ck7rBnAgSaj4ySTRWurRaR6PAX1dBd1tSX21zi3VMpRXN76ETvY8N1M25-4hdUvJSqS1ZsUXM3~4uJ6pRlnnh9DBN~Z7meISaqZepmQ60J48IGhwJnnm5IfJGpaIuuqyvLLb3hW~oRRzKTdBDx3QqpBGqCvceUZutn692S4QM5gSDrv1c~W2D6LSj92NMJPclUheeH0E2olUXN6uGBV94Om537w8n6F~0-4E7qgwJbSpdq3FA9garPv-Qsurb2AoGU~pYzNh8ey-FX8xiTjaLufHu3NgWd7Gx-d~kM2ldvrUIs3Ol965PUWIcPol6FCYL-A__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Molecular_characterization_functional_expression_tissue_localization_and_protective_potential_of_a_Taenia_solium_fatty_acid_binding_protein","translated_slug":"","page_count":9,"language":"en","content_type":"Work","summary":"The fatty acid-binding proteins (FABPs) comprise a family of proteins that are widely expressed in animal cells and perform a variety of vital functions. Here, we report the identification, characterization, recombinant expression, tissue localization and protective potential of a Taenia solium FABP (TsFABP1). The TsFABP1 primary structure showed all the conserved residues characteristic of the subfamily iv of the intracellular Lipid-Binding Proteins (iLBPs), including those involved in the binding stabilization of the fatty acid molecule. Through a competitive binding assay we found that TsFABP1 is able to bind at least six different fatty acids with preference toward palmitic and stearic acid, suggesting that TsFABP1 is a member of the iLBP subfamily iv. Immunolocalization assays carried out on larval and adult tissues of four species of taeniids using anti-TsFABP1 hyperimmune sera produced in mice and rabbit, showed intense labeling in the tegument of the spiral canal and in subtegumental cytons of the larvae. These findings suggest that the spiral canal might be a major place for FA uptake in the developing scolex. In contrast, only subtegumental cytons in the adult worms stained positive. We propose that TsFABP1 is involved in the mechanism to mobilize fatty acids between compartments in the extensive syncytial tissue of taeniids. Protection assays carried out in a murine model of cysticercosis showed that subcutaneous immunization with TsFABP1 resulted in about 45% reduction of parasite load against an intraperitoneal challenge with Taenia crassiceps cysts. This reduction in parasite load correlated with the level of cellular and humoral immune responses against TsFABP1, as determined in spleen lymphocyte proliferation and ELISA testing.","owner":{"id":36697270,"first_name":"Julio","middle_initials":null,"last_name":"Carrero","page_name":"JulioCarrero1","domain_name":"independent","created_at":"2015-10-21T12:09:01.815-07:00","display_name":"Julio Carrero","url":"https://independent.academia.edu/JulioCarrero1"},"attachments":[{"id":110480513,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/110480513/thumbnails/1.jpg","file_name":"j.molbiopara.2012.10.00220240115-1-3gb6fm.pdf","download_url":"https://www.academia.edu/attachments/110480513/download_file","bulk_download_file_name":"Molecular_characterization_functional_ex.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/110480513/j.molbiopara.2012.10.00220240115-1-3gb6fm-libre.pdf?1705355553=\u0026response-content-disposition=attachment%3B+filename%3DMolecular_characterization_functional_ex.pdf\u0026Expires=1743230062\u0026Signature=dScZ~48P6TirXJwkm4~ck7rBnAgSaj4ySTRWurRaR6PAX1dBd1tSX21zi3VMpRXN76ETvY8N1M25-4hdUvJSqS1ZsUXM3~4uJ6pRlnnh9DBN~Z7meISaqZepmQ60J48IGhwJnnm5IfJGpaIuuqyvLLb3hW~oRRzKTdBDx3QqpBGqCvceUZutn692S4QM5gSDrv1c~W2D6LSj92NMJPclUheeH0E2olUXN6uGBV94Om537w8n6F~0-4E7qgwJbSpdq3FA9garPv-Qsurb2AoGU~pYzNh8ey-FX8xiTjaLufHu3NgWd7Gx-d~kM2ldvrUIs3Ol965PUWIcPol6FCYL-A__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":145,"name":"Biochemistry","url":"https://www.academia.edu/Documents/in/Biochemistry"},{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences"},{"id":67484,"name":"Sequence alignment","url":"https://www.academia.edu/Documents/in/Sequence_alignment"},{"id":72314,"name":"Fatty acids","url":"https://www.academia.edu/Documents/in/Fatty_acids"},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice"},{"id":129391,"name":"Gene Order","url":"https://www.academia.edu/Documents/in/Gene_Order"},{"id":136892,"name":"Immunization","url":"https://www.academia.edu/Documents/in/Immunization"},{"id":809881,"name":"Amino Acid Sequence","url":"https://www.academia.edu/Documents/in/Amino_Acid_Sequence"},{"id":990417,"name":"Recombinant Proteins","url":"https://www.academia.edu/Documents/in/Recombinant_Proteins"},{"id":1010725,"name":"Protein Binding","url":"https://www.academia.edu/Documents/in/Protein_Binding"},{"id":1458519,"name":"Cysticercosis","url":"https://www.academia.edu/Documents/in/Cysticercosis"},{"id":1509323,"name":"Fatty Acid Binding Protein","url":"https://www.academia.edu/Documents/in/Fatty_Acid_Binding_Protein"},{"id":2060319,"name":"Taenia solium","url":"https://www.academia.edu/Documents/in/Taenia_solium"},{"id":2467566,"name":"Molecular Sequence Data","url":"https://www.academia.edu/Documents/in/Molecular_Sequence_Data"},{"id":3763225,"name":"Medical and Health Sciences","url":"https://www.academia.edu/Documents/in/Medical_and_Health_Sciences"}],"urls":[{"id":38604496,"url":"https://doi.org/10.1016/j.molbiopara.2012.10.002"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") if (true) { Aedu.setUpFigureCarousel('profile-work-113549620-figures'); } }); </script> <div class="js-work-strip profile--work_container" data-work-id="113549619"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/113549619/Upstream_regulatory_sequences_required_for_expression_of_the_Trichomonas_vaginalis_%CE%B1_succinyl_CoA_synthetase_gene"><img alt="Research paper thumbnail of Upstream regulatory sequences required for expression of the Trichomonas vaginalis α-succinyl CoA synthetase gene" class="work-thumbnail" src="https://attachments.academia-assets.com/110480519/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/113549619/Upstream_regulatory_sequences_required_for_expression_of_the_Trichomonas_vaginalis_%CE%B1_succinyl_CoA_synthetase_gene">Upstream regulatory sequences required for expression of the Trichomonas vaginalis α-succinyl CoA synthetase gene</a></div><div class="wp-workCard_item"><span>Molecular and Biochemical Parasitology</span><span>, Nov 1, 1999</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="2469b745ed44ba477910164395b2ae52" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":110480519,"asset_id":113549619,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/110480519/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="113549619"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="113549619"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 113549619; 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dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "2469b745ed44ba477910164395b2ae52" } } $('.js-work-strip[data-work-id=113549619]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":113549619,"title":"Upstream regulatory sequences required for expression of the Trichomonas vaginalis α-succinyl CoA synthetase gene","translated_title":"","metadata":{"publisher":"Elsevier BV","ai_abstract":"This research investigates the upstream regulatory sequences required for the expression of the Trichomonas vaginalis α-succinyl CoA synthetase (aSCS) gene. Focused on defining the core promoter characteristics, the study identifies significant deletion and mutation effects on transcriptional activity. The findings contribute to a deeper understanding of transcriptional regulation in early-branching eukaryotes and highlight parallels with higher eukaryotic systems.","publication_date":{"day":1,"month":11,"year":1999,"errors":{}},"publication_name":"Molecular and Biochemical Parasitology"},"translated_abstract":null,"internal_url":"https://www.academia.edu/113549619/Upstream_regulatory_sequences_required_for_expression_of_the_Trichomonas_vaginalis_%CE%B1_succinyl_CoA_synthetase_gene","translated_internal_url":"","created_at":"2024-01-15T12:51:34.043-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":36697270,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":110480519,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/110480519/thumbnails/1.jpg","file_name":"s0166-685128992900137-120240115-1-s4o4yh.pdf","download_url":"https://www.academia.edu/attachments/110480519/download_file","bulk_download_file_name":"Upstream_regulatory_sequences_required_f.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/110480519/s0166-685128992900137-120240115-1-s4o4yh-libre.pdf?1705355546=\u0026response-content-disposition=attachment%3B+filename%3DUpstream_regulatory_sequences_required_f.pdf\u0026Expires=1743230062\u0026Signature=Cb5it~~eFinKTKCYwesBGSwWtuZgqqUwe5~dkkL~Q3ul0640P6rNXjq2DcVTLp2dGSoWpCCLZsu1wt3N046IswnqQ8tdPuv90rrdBoTnZ3jvUR4kc8G5t2oPQm2PNFR-f381Tg2oGnbY8Dk6B7g8xawBr8uY9qIjqsMW6Yq~8uz-irLNdKszgaYZbFyAGc~tic7JtZH5ZrG2ulMijTxhaFkhG2AR3Vvmz~cxS4-3Z5lI69AsLPYLzBaBR0HzypCLCnXxMZdh4docvdH3qRtp-ZvQvIofBK56RcfUHVJb6LObHiPEUWtT7oVOx7ZtjEQiLE9ojG3L9ZdOQ7qyftdNBA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Upstream_regulatory_sequences_required_for_expression_of_the_Trichomonas_vaginalis_α_succinyl_CoA_synthetase_gene","translated_slug":"","page_count":7,"language":"en","content_type":"Work","summary":null,"owner":{"id":36697270,"first_name":"Julio","middle_initials":null,"last_name":"Carrero","page_name":"JulioCarrero1","domain_name":"independent","created_at":"2015-10-21T12:09:01.815-07:00","display_name":"Julio Carrero","url":"https://independent.academia.edu/JulioCarrero1"},"attachments":[{"id":110480519,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/110480519/thumbnails/1.jpg","file_name":"s0166-685128992900137-120240115-1-s4o4yh.pdf","download_url":"https://www.academia.edu/attachments/110480519/download_file","bulk_download_file_name":"Upstream_regulatory_sequences_required_f.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/110480519/s0166-685128992900137-120240115-1-s4o4yh-libre.pdf?1705355546=\u0026response-content-disposition=attachment%3B+filename%3DUpstream_regulatory_sequences_required_f.pdf\u0026Expires=1743230062\u0026Signature=Cb5it~~eFinKTKCYwesBGSwWtuZgqqUwe5~dkkL~Q3ul0640P6rNXjq2DcVTLp2dGSoWpCCLZsu1wt3N046IswnqQ8tdPuv90rrdBoTnZ3jvUR4kc8G5t2oPQm2PNFR-f381Tg2oGnbY8Dk6B7g8xawBr8uY9qIjqsMW6Yq~8uz-irLNdKszgaYZbFyAGc~tic7JtZH5ZrG2ulMijTxhaFkhG2AR3Vvmz~cxS4-3Z5lI69AsLPYLzBaBR0HzypCLCnXxMZdh4docvdH3qRtp-ZvQvIofBK56RcfUHVJb6LObHiPEUWtT7oVOx7ZtjEQiLE9ojG3L9ZdOQ7qyftdNBA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":156,"name":"Genetics","url":"https://www.academia.edu/Documents/in/Genetics"},{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences"},{"id":74780,"name":"Mutation","url":"https://www.academia.edu/Documents/in/Mutation"},{"id":131572,"name":"Trichomonas vaginalis","url":"https://www.academia.edu/Documents/in/Trichomonas_vaginalis"},{"id":181936,"name":"Gene","url":"https://www.academia.edu/Documents/in/Gene"},{"id":809882,"name":"Base Sequence","url":"https://www.academia.edu/Documents/in/Base_Sequence"},{"id":1763968,"name":"Gene Expression Regulation","url":"https://www.academia.edu/Documents/in/Gene_Expression_Regulation"},{"id":2467566,"name":"Molecular Sequence Data","url":"https://www.academia.edu/Documents/in/Molecular_Sequence_Data"},{"id":3763225,"name":"Medical and Health Sciences","url":"https://www.academia.edu/Documents/in/Medical_and_Health_Sciences"}],"urls":[{"id":38604495,"url":"https://doi.org/10.1016/s0166-6851(99)00137-1"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") if (false) { Aedu.setUpFigureCarousel('profile-work-113549619-figures'); } }); </script> <div class="js-work-strip profile--work_container" data-work-id="113549618"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/113549618/Intestinal_amoebiasis_160_years_of_its_first_detection_and_still_remains_as_a_health_problem_in_developing_countries"><img alt="Research paper thumbnail of Intestinal amoebiasis: 160 years of its first detection and still remains as a health problem in developing countries" class="work-thumbnail" src="https://attachments.academia-assets.com/110480515/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/113549618/Intestinal_amoebiasis_160_years_of_its_first_detection_and_still_remains_as_a_health_problem_in_developing_countries">Intestinal amoebiasis: 160 years of its first detection and still remains as a health problem in developing countries</a></div><div class="wp-workCard_item"><span>International Journal of Medical Microbiology</span><span>, 2020</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Amoebiasis is a parasitic disease caused by Entamoeba histolytica (E. histolytica), an extracellu...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Amoebiasis is a parasitic disease caused by Entamoeba histolytica (E. histolytica), an extracellular enteric protozoan. This infection mainly affects people from developing countries with limited hygiene conditions, where it is endemic. Infective cysts are transmitted by the fecal-oral route, excysting in the terminal ileum and producing invasive trophozoites (amoebae). E. histolytica mainly lives in the large intestine without causing symptoms; however, possibly as a result of so far unknown signals, the amoebae invade the mucosa and epithelium causing intestinal amoebiasis. E. histolytica possesses different mechanisms of pathogenicity for the adherence to the intestinal epithelium and for degrading extracellular matrix proteins, producing tissue lesions that progress to abscesses and a host acute inflammatory response. Much information has been obtained regarding the virulence factors, metabolism, mechanisms of pathogenicity, and the host immune response against this parasite; in addition, alternative treatments to metronidazole are continually emerging. An accesible and low-cost diagnostic method that can distinguish E. histolytica from the most nonpathogenic amoebae and an effective vaccine are necessary for protecting against amoebiasis. However, research about the disease and its prevention has been a challenge due to the relationship between E. histolytica and the host during the distinct stages of the disease is multifaceted. In this review, we analyze the interaction between the parasite, the human host, and the colon microbiota or pathogenic microorganisms, which together give rise to intestinal amoebiasis.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="baf03ac408715d395ca2404d0cd0d61a" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":110480515,"asset_id":113549618,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/110480515/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="113549618"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="113549618"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 113549618; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=113549618]").text(description); $(".js-view-count[data-work-id=113549618]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 113549618; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='113549618']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "baf03ac408715d395ca2404d0cd0d61a" } } $('.js-work-strip[data-work-id=113549618]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":113549618,"title":"Intestinal amoebiasis: 160 years of its first detection and still remains as a health problem in developing countries","translated_title":"","metadata":{"publisher":"Elsevier BV","ai_title_tag":"Amoebiasis: 160 Years of Challenges in Developing Nations","grobid_abstract":"Amoebiasis is a parasitic disease caused by Entamoeba histolytica (E. histolytica), an extracellular enteric protozoan. This infection mainly affects people from developing countries with limited hygiene conditions, where it is endemic. Infective cysts are transmitted by the fecal-oral route, excysting in the terminal ileum and producing invasive trophozoites (amoebae). E. histolytica mainly lives in the large intestine without causing symptoms; however, possibly as a result of so far unknown signals, the amoebae invade the mucosa and epithelium causing intestinal amoebiasis. E. histolytica possesses different mechanisms of pathogenicity for the adherence to the intestinal epithelium and for degrading extracellular matrix proteins, producing tissue lesions that progress to abscesses and a host acute inflammatory response. Much information has been obtained regarding the virulence factors, metabolism, mechanisms of pathogenicity, and the host immune response against this parasite; in addition, alternative treatments to metronidazole are continually emerging. An accesible and low-cost diagnostic method that can distinguish E. histolytica from the most nonpathogenic amoebae and an effective vaccine are necessary for protecting against amoebiasis. However, research about the disease and its prevention has been a challenge due to the relationship between E. histolytica and the host during the distinct stages of the disease is multifaceted. 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This infection mainly affects people from developing countries with limited hygiene conditions, where it is endemic. Infective cysts are transmitted by the fecal-oral route, excysting in the terminal ileum and producing invasive trophozoites (amoebae). E. histolytica mainly lives in the large intestine without causing symptoms; however, possibly as a result of so far unknown signals, the amoebae invade the mucosa and epithelium causing intestinal amoebiasis. E. histolytica possesses different mechanisms of pathogenicity for the adherence to the intestinal epithelium and for degrading extracellular matrix proteins, producing tissue lesions that progress to abscesses and a host acute inflammatory response. Much information has been obtained regarding the virulence factors, metabolism, mechanisms of pathogenicity, and the host immune response against this parasite; in addition, alternative treatments to metronidazole are continually emerging. An accesible and low-cost diagnostic method that can distinguish E. histolytica from the most nonpathogenic amoebae and an effective vaccine are necessary for protecting against amoebiasis. However, research about the disease and its prevention has been a challenge due to the relationship between E. histolytica and the host during the distinct stages of the disease is multifaceted. In this review, we analyze the interaction between the parasite, the human host, and the colon microbiota or pathogenic microorganisms, which together give rise to intestinal amoebiasis.","owner":{"id":36697270,"first_name":"Julio","middle_initials":null,"last_name":"Carrero","page_name":"JulioCarrero1","domain_name":"independent","created_at":"2015-10-21T12:09:01.815-07:00","display_name":"Julio Carrero","url":"https://independent.academia.edu/JulioCarrero1"},"attachments":[{"id":110480515,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/110480515/thumbnails/1.jpg","file_name":"j.ijmm.2019.15135820240115-1-855ev5.pdf","download_url":"https://www.academia.edu/attachments/110480515/download_file","bulk_download_file_name":"Intestinal_amoebiasis_160_years_of_its_f.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/110480515/j.ijmm.2019.15135820240115-1-855ev5-libre.pdf?1705355548=\u0026response-content-disposition=attachment%3B+filename%3DIntestinal_amoebiasis_160_years_of_its_f.pdf\u0026Expires=1743230062\u0026Signature=dyigC-r1CjHVWtKRY6v96uHSE7ShhnNHoxDhkl~YOlJaOa5d34ZtCAnXk47q0J92RozrxId-vP7X5Gjae5My1~L7KddBTHPq-yXODrBzsXgUAp9J~SGx6S4bYXJlQjS~cBMRLbbNVx0s50csDohLH8Kg3PG1unn90g-jpS30iDaDjy2Z0Bbw2wkk7zxRW4hz-EjmpuQc5~9AQCoZoxk1Eh4h~DNNoi0N~hAfsOOIf9KXKtNZJLMp8rqONxrqroXMTgHO3Vdp2sPRrf-UY9G567oX8WCfJQKwoeAueqzpOf~8Q~BMvSOpfAFnB-fDcP-T~qBUqBYXFLNJyUGQ-3-AiA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology"},{"id":6947,"name":"Medical Microbiology","url":"https://www.academia.edu/Documents/in/Medical_Microbiology"},{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":52873,"name":"Virulence","url":"https://www.academia.edu/Documents/in/Virulence"},{"id":133215,"name":"Amoebiasis","url":"https://www.academia.edu/Documents/in/Amoebiasis"},{"id":324154,"name":"Immune system","url":"https://www.academia.edu/Documents/in/Immune_system"},{"id":571342,"name":"Entamoeba histolytica","url":"https://www.academia.edu/Documents/in/Entamoeba_histolytica"},{"id":1942969,"name":"Parasitic Disease","url":"https://www.academia.edu/Documents/in/Parasitic_Disease"}],"urls":[{"id":38604494,"url":"https://doi.org/10.1016/j.ijmm.2019.151358"}]}, dispatcherData: dispatcherData }); 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") if (false) { Aedu.setUpFigureCarousel('profile-work-113549617-figures'); } }); </script> <div class="js-work-strip profile--work_container" data-work-id="113549616"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/113549616/Cloning_sequencing_and_functional_expression_of_cytosolic_malate_dehydrogenase_from_Taenia_solium_Purification_and_characterization_of_the_recombinant_enzyme"><img alt="Research paper thumbnail of Cloning, sequencing and functional expression of cytosolic malate dehydrogenase from Taenia solium: Purification and characterization of the recombinant enzyme" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title">Cloning, sequencing and functional expression of cytosolic malate dehydrogenase from Taenia solium: Purification and characterization of the recombinant enzyme</div><div class="wp-workCard_item"><span>Experimental Parasitology</span><span>, Jul 1, 2011</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">We report herein the complete coding sequence of a Taenia solium cytosolic malate dehydrogenase (...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">We report herein the complete coding sequence of a Taenia solium cytosolic malate dehydrogenase (TscMDH). The cDNA fragment, identified from the T. solium genome project database, encodes a protein of 332 amino acid residues with an estimated molecular weight of 36517Da. For recombinant expression, the full length coding sequence was cloned into pET23a. After successful expression and enzyme purification, isoelectrofocusing gel electrophoresis allowed to confirm the calculated pI value at 8.1, as deduced from the amino acid sequence. The recombinant protein (r-TscMDH) showed MDH activity of 409U/mg in the reduction of oxaloacetate, with neither lactate dehydrogenase activity nor NADPH selectivity. Optimum pH for enzyme activity was 7.6 for oxaloacetate reduction and 9.6 for malate oxidation. K(cat) values for oxaloacetate, malate, NAD, and NADH were 665, 47, 385, and 962s(-1), respectively. Additionally, a partial characterization of TsMDH gene structure after analysis of a 1.56Kb genomic contig assembly is also reported.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="113549616"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="113549616"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 113549616; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=113549616]").text(description); $(".js-view-count[data-work-id=113549616]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 113549616; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='113549616']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=113549616]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":113549616,"title":"Cloning, sequencing and functional expression of cytosolic malate dehydrogenase from Taenia solium: Purification and characterization of the recombinant enzyme","translated_title":"","metadata":{"abstract":"We report herein the complete coding sequence of a Taenia solium cytosolic malate dehydrogenase (TscMDH). 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Additionally, a partial characterization of TsMDH gene structure after analysis of a 1.56Kb genomic contig assembly is also reported.","publisher":"Elsevier BV","publication_date":{"day":1,"month":7,"year":2011,"errors":{}},"publication_name":"Experimental Parasitology"},"translated_abstract":"We report herein the complete coding sequence of a Taenia solium cytosolic malate dehydrogenase (TscMDH). The cDNA fragment, identified from the T. solium genome project database, encodes a protein of 332 amino acid residues with an estimated molecular weight of 36517Da. For recombinant expression, the full length coding sequence was cloned into pET23a. After successful expression and enzyme purification, isoelectrofocusing gel electrophoresis allowed to confirm the calculated pI value at 8.1, as deduced from the amino acid sequence. The recombinant protein (r-TscMDH) showed MDH activity of 409U/mg in the reduction of oxaloacetate, with neither lactate dehydrogenase activity nor NADPH selectivity. Optimum pH for enzyme activity was 7.6 for oxaloacetate reduction and 9.6 for malate oxidation. K(cat) values for oxaloacetate, malate, NAD, and NADH were 665, 47, 385, and 962s(-1), respectively. Additionally, a partial characterization of TsMDH gene structure after analysis of a 1.56Kb genomic contig assembly is also reported.","internal_url":"https://www.academia.edu/113549616/Cloning_sequencing_and_functional_expression_of_cytosolic_malate_dehydrogenase_from_Taenia_solium_Purification_and_characterization_of_the_recombinant_enzyme","translated_internal_url":"","created_at":"2024-01-15T12:51:33.660-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":36697270,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Cloning_sequencing_and_functional_expression_of_cytosolic_malate_dehydrogenase_from_Taenia_solium_Purification_and_characterization_of_the_recombinant_enzyme","translated_slug":"","page_count":null,"language":"en","content_type":"Work","summary":"We report herein the complete coding sequence of a Taenia solium cytosolic malate dehydrogenase (TscMDH). The cDNA fragment, identified from the T. solium genome project database, encodes a protein of 332 amino acid residues with an estimated molecular weight of 36517Da. For recombinant expression, the full length coding sequence was cloned into pET23a. After successful expression and enzyme purification, isoelectrofocusing gel electrophoresis allowed to confirm the calculated pI value at 8.1, as deduced from the amino acid sequence. The recombinant protein (r-TscMDH) showed MDH activity of 409U/mg in the reduction of oxaloacetate, with neither lactate dehydrogenase activity nor NADPH selectivity. Optimum pH for enzyme activity was 7.6 for oxaloacetate reduction and 9.6 for malate oxidation. K(cat) values for oxaloacetate, malate, NAD, and NADH were 665, 47, 385, and 962s(-1), respectively. Additionally, a partial characterization of TsMDH gene structure after analysis of a 1.56Kb genomic contig assembly is also reported.","owner":{"id":36697270,"first_name":"Julio","middle_initials":null,"last_name":"Carrero","page_name":"JulioCarrero1","domain_name":"independent","created_at":"2015-10-21T12:09:01.815-07:00","display_name":"Julio Carrero","url":"https://independent.academia.edu/JulioCarrero1"},"attachments":[],"research_interests":[{"id":145,"name":"Biochemistry","url":"https://www.academia.edu/Documents/in/Biochemistry"},{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology"},{"id":2513,"name":"Molecular Biology","url":"https://www.academia.edu/Documents/in/Molecular_Biology"},{"id":4987,"name":"Kinetics","url":"https://www.academia.edu/Documents/in/Kinetics"},{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":53307,"name":"Experimental parasitology","url":"https://www.academia.edu/Documents/in/Experimental_parasitology"},{"id":76407,"name":"Circular Dichroism","url":"https://www.academia.edu/Documents/in/Circular_Dichroism"},{"id":224866,"name":"Gene Structure","url":"https://www.academia.edu/Documents/in/Gene_Structure"},{"id":231661,"name":"Enzyme","url":"https://www.academia.edu/Documents/in/Enzyme"},{"id":583568,"name":"Enzyme activity","url":"https://www.academia.edu/Documents/in/Enzyme_activity"},{"id":695018,"name":"Molecular weight","url":"https://www.academia.edu/Documents/in/Molecular_weight"},{"id":759403,"name":"Gel electrophoresis","url":"https://www.academia.edu/Documents/in/Gel_electrophoresis"},{"id":809881,"name":"Amino Acid Sequence","url":"https://www.academia.edu/Documents/in/Amino_Acid_Sequence"},{"id":809882,"name":"Base Sequence","url":"https://www.academia.edu/Documents/in/Base_Sequence"},{"id":828507,"name":"NAD","url":"https://www.academia.edu/Documents/in/NAD"},{"id":836013,"name":"Lactate dehydrogenase","url":"https://www.academia.edu/Documents/in/Lactate_dehydrogenase"},{"id":1416887,"name":"Citrate Synthase","url":"https://www.academia.edu/Documents/in/Citrate_Synthase"},{"id":2467566,"name":"Molecular Sequence Data","url":"https://www.academia.edu/Documents/in/Molecular_Sequence_Data"}],"urls":[{"id":38604492,"url":"https://doi.org/10.1016/j.exppara.2011.03.008"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") if (false) { Aedu.setUpFigureCarousel('profile-work-113549616-figures'); } }); </script> <div class="js-work-strip profile--work_container" data-work-id="113549615"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/113549615/Host_Immune_Responses_Against_Intestinal_Unicellular_Parasites_and_Their_Role_in_Pathogenesis_and_Protection"><img alt="Research paper thumbnail of Host Immune Responses Against Intestinal Unicellular Parasites and Their Role in Pathogenesis and Protection" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title">Host Immune Responses Against Intestinal Unicellular Parasites and Their Role in Pathogenesis and Protection</div><div class="wp-workCard_item"><span>Elsevier eBooks</span><span>, 2022</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="113549615"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="113549615"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 113549615; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=113549615]").text(description); $(".js-view-count[data-work-id=113549615]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 113549615; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='113549615']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") if (false) { Aedu.setUpFigureCarousel('profile-work-113549615-figures'); } }); </script> <div class="js-work-strip profile--work_container" data-work-id="113549614"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/113549614/Effects_of_solvent_free_amine_functionalization_of_graphene_oxide_and_nanodiamond_on_bacterial_growth"><img alt="Research paper thumbnail of Effects of solvent-free amine functionalization of graphene oxide and nanodiamond on bacterial growth" class="work-thumbnail" src="https://attachments.academia-assets.com/110480511/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/113549614/Effects_of_solvent_free_amine_functionalization_of_graphene_oxide_and_nanodiamond_on_bacterial_growth">Effects of solvent-free amine functionalization of graphene oxide and nanodiamond on bacterial growth</a></div><div class="wp-workCard_item"><span>Fullerenes Nanotubes and Carbon Nanostructures</span><span>, Aug 24, 2020</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">We studied the effect of covalent functionalization of graphene oxide (GO) and nanodiamond (ND) w...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">We studied the effect of covalent functionalization of graphene oxide (GO) and nanodiamond (ND) with octadecylamine (ODA) on bacterial growth (a series of experiments was performed also with pristine single-walled carbon nanotubes [SWNTs] for comparison). The bacteria tested were Escherichia coli and Staphylococcus aureus, which represent Gram-positive and Gram-negative types, respectively, and are of importance for the environment and human health. We found that pristine GO is the most toxic nanomaterial in both bacteria species, which exhibits a dose-dependent behavior. SWNTs show toxicity only against S. aureus at the higher concentrations of 1.0 and 10 mg/mL. Pristine ND, as expected, was found to be the least toxic against both species of bacteria, and in the experiments with S. aureus it even showed a viability amplifier activity at 10 mg/ mL concentration. The use of ODA-functionalized nanomaterials generally changed the toxicity behavior, neutralizing the antibacterial effect of GO (for both S. aureus and E. coli), but making ODA-functionalized ND more toxic as compared to pristine material (with respect to S. aureus).</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="d9d734aa368e1a08f97877827a89b04b" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":110480511,"asset_id":113549614,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/110480511/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="113549614"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="113549614"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 113549614; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=113549614]").text(description); $(".js-view-count[data-work-id=113549614]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 113549614; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='113549614']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "d9d734aa368e1a08f97877827a89b04b" } } $('.js-work-strip[data-work-id=113549614]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":113549614,"title":"Effects of solvent-free amine functionalization of graphene oxide and nanodiamond on bacterial growth","translated_title":"","metadata":{"publisher":"Taylor \u0026 Francis","ai_title_tag":"Amine Functionalization of Graphene Oxide Effects","grobid_abstract":"We studied the effect of covalent functionalization of graphene oxide (GO) and nanodiamond (ND) with octadecylamine (ODA) on bacterial growth (a series of experiments was performed also with pristine single-walled carbon nanotubes [SWNTs] for comparison). The bacteria tested were Escherichia coli and Staphylococcus aureus, which represent Gram-positive and Gram-negative types, respectively, and are of importance for the environment and human health. We found that pristine GO is the most toxic nanomaterial in both bacteria species, which exhibits a dose-dependent behavior. SWNTs show toxicity only against S. aureus at the higher concentrations of 1.0 and 10 mg/mL. Pristine ND, as expected, was found to be the least toxic against both species of bacteria, and in the experiments with S. aureus it even showed a viability amplifier activity at 10 mg/ mL concentration. The use of ODA-functionalized nanomaterials generally changed the toxicity behavior, neutralizing the antibacterial effect of GO (for both S. aureus and E. coli), but making ODA-functionalized ND more toxic as compared to pristine material (with respect to S. aureus).","publication_date":{"day":24,"month":8,"year":2020,"errors":{}},"publication_name":"Fullerenes Nanotubes and Carbon Nanostructures","grobid_abstract_attachment_id":110480511},"translated_abstract":null,"internal_url":"https://www.academia.edu/113549614/Effects_of_solvent_free_amine_functionalization_of_graphene_oxide_and_nanodiamond_on_bacterial_growth","translated_internal_url":"","created_at":"2024-01-15T12:51:33.398-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":36697270,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":110480511,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/110480511/thumbnails/1.jpg","file_name":"1536383X.2020.181123520240115-1-z64ulg.pdf","download_url":"https://www.academia.edu/attachments/110480511/download_file","bulk_download_file_name":"Effects_of_solvent_free_amine_functional.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/110480511/1536383X.2020.181123520240115-1-z64ulg-libre.pdf?1705355552=\u0026response-content-disposition=attachment%3B+filename%3DEffects_of_solvent_free_amine_functional.pdf\u0026Expires=1743230063\u0026Signature=hOVxEPf4y-Y3M9dg26uEo2N7ALYNc2TO6gL1KxhzzYS8BMnxkejIS2n30Cm9-RZ-13sMVLYDTr6LFFwjEaArFYArNHTwUYTBBB7Eiih3prGFO7Fg3M2rnJwiISgu-jXA4K0v2BXlAVJKtb1IU~TV0XbTgz1E4sS-gVK-KR6noXXrjm3JGdTTXBMCYo6v2DdtDYxmFJKoyIumK7i0pQEOX850bwPPTAKro~xOfL8FbLPVn4loGgyStZxhyIuV628jAfOHtYAeMlHdbtOhPa3TxC11f6oiRRF4ddZXRcN4h3g9mRvt0z-OtNiQ3U93qvLjPH7t8N4KbLys8f-EBbJQbw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Effects_of_solvent_free_amine_functionalization_of_graphene_oxide_and_nanodiamond_on_bacterial_growth","translated_slug":"","page_count":10,"language":"en","content_type":"Work","summary":"We studied the effect of covalent functionalization of graphene oxide (GO) and nanodiamond (ND) with octadecylamine (ODA) on bacterial growth (a series of experiments was performed also with pristine single-walled carbon nanotubes [SWNTs] for comparison). The bacteria tested were Escherichia coli and Staphylococcus aureus, which represent Gram-positive and Gram-negative types, respectively, and are of importance for the environment and human health. We found that pristine GO is the most toxic nanomaterial in both bacteria species, which exhibits a dose-dependent behavior. SWNTs show toxicity only against S. aureus at the higher concentrations of 1.0 and 10 mg/mL. Pristine ND, as expected, was found to be the least toxic against both species of bacteria, and in the experiments with S. aureus it even showed a viability amplifier activity at 10 mg/ mL concentration. The use of ODA-functionalized nanomaterials generally changed the toxicity behavior, neutralizing the antibacterial effect of GO (for both S. aureus and E. coli), but making ODA-functionalized ND more toxic as compared to pristine material (with respect to S. aureus).","owner":{"id":36697270,"first_name":"Julio","middle_initials":null,"last_name":"Carrero","page_name":"JulioCarrero1","domain_name":"independent","created_at":"2015-10-21T12:09:01.815-07:00","display_name":"Julio Carrero","url":"https://independent.academia.edu/JulioCarrero1"},"attachments":[{"id":110480511,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/110480511/thumbnails/1.jpg","file_name":"1536383X.2020.181123520240115-1-z64ulg.pdf","download_url":"https://www.academia.edu/attachments/110480511/download_file","bulk_download_file_name":"Effects_of_solvent_free_amine_functional.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/110480511/1536383X.2020.181123520240115-1-z64ulg-libre.pdf?1705355552=\u0026response-content-disposition=attachment%3B+filename%3DEffects_of_solvent_free_amine_functional.pdf\u0026Expires=1743230063\u0026Signature=hOVxEPf4y-Y3M9dg26uEo2N7ALYNc2TO6gL1KxhzzYS8BMnxkejIS2n30Cm9-RZ-13sMVLYDTr6LFFwjEaArFYArNHTwUYTBBB7Eiih3prGFO7Fg3M2rnJwiISgu-jXA4K0v2BXlAVJKtb1IU~TV0XbTgz1E4sS-gVK-KR6noXXrjm3JGdTTXBMCYo6v2DdtDYxmFJKoyIumK7i0pQEOX850bwPPTAKro~xOfL8FbLPVn4loGgyStZxhyIuV628jAfOHtYAeMlHdbtOhPa3TxC11f6oiRRF4ddZXRcN4h3g9mRvt0z-OtNiQ3U93qvLjPH7t8N4KbLys8f-EBbJQbw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":511,"name":"Materials Science","url":"https://www.academia.edu/Documents/in/Materials_Science"},{"id":523,"name":"Chemistry","url":"https://www.academia.edu/Documents/in/Chemistry"},{"id":11541,"name":"Graphene","url":"https://www.academia.edu/Documents/in/Graphene"},{"id":11973,"name":"Nanomaterials","url":"https://www.academia.edu/Documents/in/Nanomaterials"},{"id":17733,"name":"Nanotechnology","url":"https://www.academia.edu/Documents/in/Nanotechnology"},{"id":79275,"name":"Surface modification","url":"https://www.academia.edu/Documents/in/Surface_modification"},{"id":113903,"name":"Bacteria","url":"https://www.academia.edu/Documents/in/Bacteria"},{"id":116078,"name":"Staphylococcus aureus","url":"https://www.academia.edu/Documents/in/Staphylococcus_aureus"},{"id":290432,"name":"Antibacterial activity","url":"https://www.academia.edu/Documents/in/Antibacterial_activity"},{"id":3276289,"name":"Nanodiamond","url":"https://www.academia.edu/Documents/in/Nanodiamond"}],"urls":[{"id":38604490,"url":"https://doi.org/10.1080/1536383x.2020.1811235"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") if (false) { Aedu.setUpFigureCarousel('profile-work-113549614-figures'); } }); </script> <div class="js-work-strip profile--work_container" data-work-id="113549613"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/113549613/Field_study_of_parasitic_contamination_of_fruits_vegetables_and_leafy_greens_in_the_Ecuadorian_Andes"><img alt="Research paper thumbnail of Field study of parasitic contamination of fruits, vegetables and leafy greens in the Ecuadorian Andes" class="work-thumbnail" src="https://attachments.academia-assets.com/110480458/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/113549613/Field_study_of_parasitic_contamination_of_fruits_vegetables_and_leafy_greens_in_the_Ecuadorian_Andes">Field study of parasitic contamination of fruits, vegetables and leafy greens in the Ecuadorian Andes</a></div><div class="wp-workCard_item"><span>F1000Research</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Background: Raw vegetables have been considered vehicles of enteroparasites. South American count...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Background: Raw vegetables have been considered vehicles of enteroparasites. South American countries are among the most important exporters of fresh vegetables; Ecuador has tropical climates and soils rich in organic matter that allow it to harvest throughout the year for sale to different countries. The aim of the study was to assess the occurrence of the parasitic contamination of fruits, vegetables and leafy greens grown in an agricultural area of the Ecuadorian Andes. Methods: A field study, cross-sectional, snowball sampling was conducted on 1,416 samples (516 fruits, 488 vegetables, and 412 leafy greens). Each sample were washed with water, and the resulting solution after removing the vegetables, was subjected to 24-hour sedimentation. The concentrated sediment underwent microscopic analysis. Results: Parasites were detected in 63.4% of the samples, leafy greens were the most contaminated (76.9%) (P&lt;0.0001), (vegetables 67.8% and fruit 48.4%), of these, cabbage (100%), on...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="8da4e6669d8ed056cda05c39d3288082" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":110480458,"asset_id":113549613,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/110480458/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="113549613"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="113549613"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 113549613; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=113549613]").text(description); $(".js-view-count[data-work-id=113549613]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 113549613; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='113549613']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "8da4e6669d8ed056cda05c39d3288082" } } $('.js-work-strip[data-work-id=113549613]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":113549613,"title":"Field study of parasitic contamination of fruits, vegetables and leafy greens in the Ecuadorian Andes","translated_title":"","metadata":{"abstract":"Background: Raw vegetables have been considered vehicles of enteroparasites. South American countries are among the most important exporters of fresh vegetables; Ecuador has tropical climates and soils rich in organic matter that allow it to harvest throughout the year for sale to different countries. The aim of the study was to assess the occurrence of the parasitic contamination of fruits, vegetables and leafy greens grown in an agricultural area of the Ecuadorian Andes. Methods: A field study, cross-sectional, snowball sampling was conducted on 1,416 samples (516 fruits, 488 vegetables, and 412 leafy greens). Each sample were washed with water, and the resulting solution after removing the vegetables, was subjected to 24-hour sedimentation. The concentrated sediment underwent microscopic analysis. Results: Parasites were detected in 63.4% of the samples, leafy greens were the most contaminated (76.9%) (P\u0026lt;0.0001), (vegetables 67.8% and fruit 48.4%), of these, cabbage (100%), on...","publisher":"F1000 Research Ltd","publication_name":"F1000Research"},"translated_abstract":"Background: Raw vegetables have been considered vehicles of enteroparasites. South American countries are among the most important exporters of fresh vegetables; Ecuador has tropical climates and soils rich in organic matter that allow it to harvest throughout the year for sale to different countries. The aim of the study was to assess the occurrence of the parasitic contamination of fruits, vegetables and leafy greens grown in an agricultural area of the Ecuadorian Andes. Methods: A field study, cross-sectional, snowball sampling was conducted on 1,416 samples (516 fruits, 488 vegetables, and 412 leafy greens). Each sample were washed with water, and the resulting solution after removing the vegetables, was subjected to 24-hour sedimentation. The concentrated sediment underwent microscopic analysis. Results: Parasites were detected in 63.4% of the samples, leafy greens were the most contaminated (76.9%) (P\u0026lt;0.0001), (vegetables 67.8% and fruit 48.4%), of these, cabbage (100%), on...","internal_url":"https://www.academia.edu/113549613/Field_study_of_parasitic_contamination_of_fruits_vegetables_and_leafy_greens_in_the_Ecuadorian_Andes","translated_internal_url":"","created_at":"2024-01-15T12:51:33.222-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":36697270,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":110480458,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/110480458/thumbnails/1.jpg","file_name":"pdf.pdf","download_url":"https://www.academia.edu/attachments/110480458/download_file","bulk_download_file_name":"Field_study_of_parasitic_contamination_o.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/110480458/pdf-libre.pdf?1705355557=\u0026response-content-disposition=attachment%3B+filename%3DField_study_of_parasitic_contamination_o.pdf\u0026Expires=1743230063\u0026Signature=F7feIGt5yB7W8AvzhzhBr0qgNw5pRrxbjhdhc4EubTrTtwlPZPBnu0Vg~se5IFo-eUZU3ymNFu9ZS4fMvp~iHSimVberjr4zPhIRj8Sc-YvC-8k~lQkL-lORkC1GY1DjyuG4-Lg6efkYMldkWKdqZrER0yqtiRNJ4BYavYVps71N0uId-3nUba8-CCqCIROEDDqckVMwd06DTFNY-xzDeQNYl9gHh5WLk6DQp4c1gciKjX3IHPZgKtjVuAaDGmbFs2GtJWxBza7PLZWehZQxXrbWXh2qkWPS0o~DNtRM6O9RcHYUXMS8ejqJ5itLxv8p3c11l-CykK9wv6sfMKp34g__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Field_study_of_parasitic_contamination_of_fruits_vegetables_and_leafy_greens_in_the_Ecuadorian_Andes","translated_slug":"","page_count":16,"language":"en","content_type":"Work","summary":"Background: Raw vegetables have been considered vehicles of enteroparasites. South American countries are among the most important exporters of fresh vegetables; Ecuador has tropical climates and soils rich in organic matter that allow it to harvest throughout the year for sale to different countries. The aim of the study was to assess the occurrence of the parasitic contamination of fruits, vegetables and leafy greens grown in an agricultural area of the Ecuadorian Andes. Methods: A field study, cross-sectional, snowball sampling was conducted on 1,416 samples (516 fruits, 488 vegetables, and 412 leafy greens). Each sample were washed with water, and the resulting solution after removing the vegetables, was subjected to 24-hour sedimentation. The concentrated sediment underwent microscopic analysis. Results: Parasites were detected in 63.4% of the samples, leafy greens were the most contaminated (76.9%) (P\u0026lt;0.0001), (vegetables 67.8% and fruit 48.4%), of these, cabbage (100%), on...","owner":{"id":36697270,"first_name":"Julio","middle_initials":null,"last_name":"Carrero","page_name":"JulioCarrero1","domain_name":"independent","created_at":"2015-10-21T12:09:01.815-07:00","display_name":"Julio Carrero","url":"https://independent.academia.edu/JulioCarrero1"},"attachments":[{"id":110480458,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/110480458/thumbnails/1.jpg","file_name":"pdf.pdf","download_url":"https://www.academia.edu/attachments/110480458/download_file","bulk_download_file_name":"Field_study_of_parasitic_contamination_o.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/110480458/pdf-libre.pdf?1705355557=\u0026response-content-disposition=attachment%3B+filename%3DField_study_of_parasitic_contamination_o.pdf\u0026Expires=1743230063\u0026Signature=F7feIGt5yB7W8AvzhzhBr0qgNw5pRrxbjhdhc4EubTrTtwlPZPBnu0Vg~se5IFo-eUZU3ymNFu9ZS4fMvp~iHSimVberjr4zPhIRj8Sc-YvC-8k~lQkL-lORkC1GY1DjyuG4-Lg6efkYMldkWKdqZrER0yqtiRNJ4BYavYVps71N0uId-3nUba8-CCqCIROEDDqckVMwd06DTFNY-xzDeQNYl9gHh5WLk6DQp4c1gciKjX3IHPZgKtjVuAaDGmbFs2GtJWxBza7PLZWehZQxXrbWXh2qkWPS0o~DNtRM6O9RcHYUXMS8ejqJ5itLxv8p3c11l-CykK9wv6sfMKp34g__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"},{"id":110480459,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/110480459/thumbnails/1.jpg","file_name":"pdf.pdf","download_url":"https://www.academia.edu/attachments/110480459/download_file","bulk_download_file_name":"Field_study_of_parasitic_contamination_o.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/110480459/pdf-libre.pdf?1705355554=\u0026response-content-disposition=attachment%3B+filename%3DField_study_of_parasitic_contamination_o.pdf\u0026Expires=1743230063\u0026Signature=Vv8pPVDpcgOxt-lK-UwzuRRts84EUKBAPFysqh5QCZeuuwP3Rrp49LgdF8pv27FV-xJJJOaPJwfI5YvcTpPKp1-eFgXpS5I~JUSiZbEniboUnms19AfLSoPufzU0VrL8p8Av44saE8robQ~YMffystUR3dV2f4U8u5qmq4hy64sGiQHORHUYH-iGtT7O7YF2IonCaWxJmf3JT0JmjBTGnBnkIPfYafE73kQnPBvZ8yqOC4bW~8OicFy1kIJFglSxlwzkHE0VPWwRS1YYqu4ySnj7Y0zwz-kMiY4G2u-y5dwnQq8Q1SKkiSEJ~iRp5SxK1aUmrIaxBO-utBXFoA~0Hw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":24173,"name":"F Mri Research","url":"https://www.academia.edu/Documents/in/F_Mri_Research"}],"urls":[{"id":38604489,"url":"https://f1000research.com/articles/12-532/v1/pdf"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") if (false) { Aedu.setUpFigureCarousel('profile-work-113549613-figures'); } }); </script> <div class="js-work-strip profile--work_container" data-work-id="113549612"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/113549612/Towards_the_development_of_an_epitope_focused_vaccine_for_SARS_CoV_2"><img alt="Research paper thumbnail of Towards the development of an epitope-focused vaccine for SARS-CoV-2" class="work-thumbnail" src="https://attachments.academia-assets.com/110480518/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/113549612/Towards_the_development_of_an_epitope_focused_vaccine_for_SARS_CoV_2">Towards the development of an epitope-focused vaccine for SARS-CoV-2</a></div><div class="wp-workCard_item"><span>Vaccine</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="f3a345f277f9bdc25d9e3379a8dfd122" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":110480518,"asset_id":113549612,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/110480518/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="113549612"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="113549612"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 113549612; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=113549612]").text(description); $(".js-view-count[data-work-id=113549612]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 113549612; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='113549612']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); 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The peptide P5 was demonstrated to be safe and immunogenic, inducing specific IgG antibodies in animal models. Although neutralizing antibody titers were low, findings support P5's potential in designing a vaccine, with plans to enhance immunogenicity by incorporating more epitopes.","publication_name":"Vaccine"},"translated_abstract":null,"internal_url":"https://www.academia.edu/113549612/Towards_the_development_of_an_epitope_focused_vaccine_for_SARS_CoV_2","translated_internal_url":"","created_at":"2024-01-15T12:51:33.036-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":36697270,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":110480518,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/110480518/thumbnails/1.jpg","file_name":"pdf.pdf","download_url":"https://www.academia.edu/attachments/110480518/download_file","bulk_download_file_name":"Towards_the_development_of_an_epitope_fo.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/110480518/pdf-libre.pdf?1705355548=\u0026response-content-disposition=attachment%3B+filename%3DTowards_the_development_of_an_epitope_fo.pdf\u0026Expires=1743230063\u0026Signature=G89ZN5ovaEEUbj8kFHtI~a9gYqsAmSchVvx4gzuSGNdTOOd23PqYdPoSWxql5s8Q0oqmuSsW6Z~eXpC85c--ZQqjVr8CRibEH-aWWsGjHI3dRjJmzEna9Ctc1otIfjXtSyuuEZumZaG9F6iW048SOhhC-h-8Q6sBFOSPeK2bWCbGk35TyhOg4ryQwzAJKbsle2BnYWrY5Ogfi4aOvOW10WI2YO-ul4-FwlXF0Bru6bn4ajqeq8Q9apGiEH0n28E-U0r0h3CX9ADiA~zwcTddjPshfgXaTJP--27lY24K9a7sU03wqdUgDgiuyDeTfnGckSxQ1Dtpz35HyHdOReuGJw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Towards_the_development_of_an_epitope_focused_vaccine_for_SARS_CoV_2","translated_slug":"","page_count":11,"language":"en","content_type":"Work","summary":null,"owner":{"id":36697270,"first_name":"Julio","middle_initials":null,"last_name":"Carrero","page_name":"JulioCarrero1","domain_name":"independent","created_at":"2015-10-21T12:09:01.815-07:00","display_name":"Julio Carrero","url":"https://independent.academia.edu/JulioCarrero1"},"attachments":[{"id":110480518,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/110480518/thumbnails/1.jpg","file_name":"pdf.pdf","download_url":"https://www.academia.edu/attachments/110480518/download_file","bulk_download_file_name":"Towards_the_development_of_an_epitope_fo.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/110480518/pdf-libre.pdf?1705355548=\u0026response-content-disposition=attachment%3B+filename%3DTowards_the_development_of_an_epitope_fo.pdf\u0026Expires=1743230063\u0026Signature=G89ZN5ovaEEUbj8kFHtI~a9gYqsAmSchVvx4gzuSGNdTOOd23PqYdPoSWxql5s8Q0oqmuSsW6Z~eXpC85c--ZQqjVr8CRibEH-aWWsGjHI3dRjJmzEna9Ctc1otIfjXtSyuuEZumZaG9F6iW048SOhhC-h-8Q6sBFOSPeK2bWCbGk35TyhOg4ryQwzAJKbsle2BnYWrY5Ogfi4aOvOW10WI2YO-ul4-FwlXF0Bru6bn4ajqeq8Q9apGiEH0n28E-U0r0h3CX9ADiA~zwcTddjPshfgXaTJP--27lY24K9a7sU03wqdUgDgiuyDeTfnGckSxQ1Dtpz35HyHdOReuGJw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":8089,"name":"Virology","url":"https://www.academia.edu/Documents/in/Virology"},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences"},{"id":150847,"name":"Vaccine","url":"https://www.academia.edu/Documents/in/Vaccine"},{"id":357811,"name":"Antibody","url":"https://www.academia.edu/Documents/in/Antibody"},{"id":1314192,"name":"Immunogenicity","url":"https://www.academia.edu/Documents/in/Immunogenicity"},{"id":1426713,"name":"Antigen","url":"https://www.academia.edu/Documents/in/Antigen"},{"id":3398462,"name":"epitope","url":"https://www.academia.edu/Documents/in/epitope"},{"id":3763225,"name":"Medical and Health Sciences","url":"https://www.academia.edu/Documents/in/Medical_and_Health_Sciences"}],"urls":[{"id":38604488,"url":"https://api.elsevier.com/content/article/PII:S0264410X22011689?httpAccept=text/xml"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") if (false) { Aedu.setUpFigureCarousel('profile-work-113549612-figures'); } }); </script> <div class="js-work-strip profile--work_container" data-work-id="113549611"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/113549611/Adsorption_of_Phosphate_and_Nitrate_Ions_on_Oxidic_Substrates_Prepared_with_a_Variable_Charge_Lithological_Material"><img alt="Research paper thumbnail of Adsorption of Phosphate and Nitrate Ions on Oxidic Substrates Prepared with a Variable-Charge Lithological Material" class="work-thumbnail" src="https://attachments.academia-assets.com/110480457/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/113549611/Adsorption_of_Phosphate_and_Nitrate_Ions_on_Oxidic_Substrates_Prepared_with_a_Variable_Charge_Lithological_Material">Adsorption of Phosphate and Nitrate Ions on Oxidic Substrates Prepared with a Variable-Charge Lithological Material</a></div><div class="wp-workCard_item"><span>Water</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">This work evaluates phosphate and nitrate ion adsorption from aqueous solutions on calcined adsor...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">This work evaluates phosphate and nitrate ion adsorption from aqueous solutions on calcined adsorbent substrates of variable charge, prepared from three granulometric fractions of an oxidic lithological material. The adsorbent material was chemically characterized, and N2 gas adsorption (BET), X-ray diffraction, and DTA techniques were applied. The experimental conditions included the protonation of the beds with HCl and H2SO4 and the study of adsorption isotherms and kinetics. The lithological material was moderately acidic (pH 5) with very little solubility (electrical conductivity 0.013 dS m−1) and a low cation exchange capacity (53.67 cmol (+) kg−1). The protonation reaction was more efficient with HCl averaging 0.745 mmol versus 0.306 mmol with H2SO4. Likewise, the HCl-treated bed showed a better adsorption of PO4−3 ions (3.296 mg/100 g bed) compared to the H2SO4-treated bed (2.579 mg/100 g bed). The isotherms showed great affinity of the PO4−3 ions with the oxide surface, and ...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="67f311868a9c6d4211ca4d4d3dcca3f0" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":110480457,"asset_id":113549611,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/110480457/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="113549611"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="113549611"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 113549611; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=113549611]").text(description); $(".js-view-count[data-work-id=113549611]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 113549611; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='113549611']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "67f311868a9c6d4211ca4d4d3dcca3f0" } } $('.js-work-strip[data-work-id=113549611]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":113549611,"title":"Adsorption of Phosphate and Nitrate Ions on Oxidic Substrates Prepared with a Variable-Charge Lithological Material","translated_title":"","metadata":{"abstract":"This work evaluates phosphate and nitrate ion adsorption from aqueous solutions on calcined adsorbent substrates of variable charge, prepared from three granulometric fractions of an oxidic lithological material. 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The isotherms showed great affinity of the PO4−3 ions with the oxide surface, and ...","publisher":"MDPI AG","publication_name":"Water"},"translated_abstract":"This work evaluates phosphate and nitrate ion adsorption from aqueous solutions on calcined adsorbent substrates of variable charge, prepared from three granulometric fractions of an oxidic lithological material. The adsorbent material was chemically characterized, and N2 gas adsorption (BET), X-ray diffraction, and DTA techniques were applied. The experimental conditions included the protonation of the beds with HCl and H2SO4 and the study of adsorption isotherms and kinetics. The lithological material was moderately acidic (pH 5) with very little solubility (electrical conductivity 0.013 dS m−1) and a low cation exchange capacity (53.67 cmol (+) kg−1). The protonation reaction was more efficient with HCl averaging 0.745 mmol versus 0.306 mmol with H2SO4. Likewise, the HCl-treated bed showed a better adsorption of PO4−3 ions (3.296 mg/100 g bed) compared to the H2SO4-treated bed (2.579 mg/100 g bed). 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") if (false) { Aedu.setUpFigureCarousel('profile-work-113549611-figures'); } }); </script> <div class="js-work-strip profile--work_container" data-work-id="113549610"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/113549610/The_Genomes_of_Two_Strains_of_Taenia_crassiceps_the_Animal_Model_for_the_Study_of_Human_Cysticercosis"><img alt="Research paper thumbnail of The Genomes of Two Strains of Taenia crassiceps the Animal Model for the Study of Human Cysticercosis" class="work-thumbnail" src="https://attachments.academia-assets.com/110480520/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/113549610/The_Genomes_of_Two_Strains_of_Taenia_crassiceps_the_Animal_Model_for_the_Study_of_Human_Cysticercosis">The Genomes of Two Strains of Taenia crassiceps the Animal Model for the Study of Human Cysticercosis</a></div><div class="wp-workCard_item"><span>Frontiers in Cellular and Infection Microbiology</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Human cysticercosis by Taenia solium is the major cause of neurological illness in countries of A...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Human cysticercosis by Taenia solium is the major cause of neurological illness in countries of Africa, Southeast Asia, and the Americas. Publication of four cestode genomes (T. solium, Echinococcus multilocularis, E. granulosus and Hymenolepis microstoma) in the last decade, marked the advent of novel approaches on the study of the host-parasite molecular crosstalk for cestode parasites of importance for human and animal health. Taenia crassiceps is another cestode parasite, closely related to T. solium, which has been used in numerous studies as an animal model for human cysticercosis. Therefore, characterization of the T. crassiceps genome will also contribute to the understanding of the human infection. Here, we report the genome of T. crassiceps WFU strain, reconstructed to a noncontiguous finished resolution and performed a genomic and differential expression comparison analysis against ORF strain. Both strain genomes were sequenced using Oxford Nanopore (MinION) and Illumina ...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="11ab74b0fcb795ac45f86de706fc3da6" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":110480520,"asset_id":113549610,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/110480520/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="113549610"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="113549610"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 113549610; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=113549610]").text(description); $(".js-view-count[data-work-id=113549610]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 113549610; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='113549610']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "11ab74b0fcb795ac45f86de706fc3da6" } } $('.js-work-strip[data-work-id=113549610]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":113549610,"title":"The Genomes of Two Strains of Taenia crassiceps the Animal Model for the Study of Human Cysticercosis","translated_title":"","metadata":{"abstract":"Human cysticercosis by Taenia solium is the major cause of neurological illness in countries of Africa, Southeast Asia, and the Americas. Publication of four cestode genomes (T. solium, Echinococcus multilocularis, E. granulosus and Hymenolepis microstoma) in the last decade, marked the advent of novel approaches on the study of the host-parasite molecular crosstalk for cestode parasites of importance for human and animal health. Taenia crassiceps is another cestode parasite, closely related to T. solium, which has been used in numerous studies as an animal model for human cysticercosis. Therefore, characterization of the T. crassiceps genome will also contribute to the understanding of the human infection. Here, we report the genome of T. crassiceps WFU strain, reconstructed to a noncontiguous finished resolution and performed a genomic and differential expression comparison analysis against ORF strain. Both strain genomes were sequenced using Oxford Nanopore (MinION) and Illumina ...","publisher":"Frontiers Media SA","ai_title_tag":"Genome Analysis of Taenia crassiceps Strains","publication_name":"Frontiers in Cellular and Infection Microbiology"},"translated_abstract":"Human cysticercosis by Taenia solium is the major cause of neurological illness in countries of Africa, Southeast Asia, and the Americas. Publication of four cestode genomes (T. solium, Echinococcus multilocularis, E. granulosus and Hymenolepis microstoma) in the last decade, marked the advent of novel approaches on the study of the host-parasite molecular crosstalk for cestode parasites of importance for human and animal health. Taenia crassiceps is another cestode parasite, closely related to T. solium, which has been used in numerous studies as an animal model for human cysticercosis. Therefore, characterization of the T. crassiceps genome will also contribute to the understanding of the human infection. Here, we report the genome of T. crassiceps WFU strain, reconstructed to a noncontiguous finished resolution and performed a genomic and differential expression comparison analysis against ORF strain. Both strain genomes were sequenced using Oxford Nanopore (MinION) and Illumina ...","internal_url":"https://www.academia.edu/113549610/The_Genomes_of_Two_Strains_of_Taenia_crassiceps_the_Animal_Model_for_the_Study_of_Human_Cysticercosis","translated_internal_url":"","created_at":"2024-01-15T12:51:32.779-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":36697270,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":110480520,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/110480520/thumbnails/1.jpg","file_name":"pdf.pdf","download_url":"https://www.academia.edu/attachments/110480520/download_file","bulk_download_file_name":"The_Genomes_of_Two_Strains_of_Taenia_cra.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/110480520/pdf-libre.pdf?1705355551=\u0026response-content-disposition=attachment%3B+filename%3DThe_Genomes_of_Two_Strains_of_Taenia_cra.pdf\u0026Expires=1743230063\u0026Signature=OR6uGi7KdO30BCljSus-0BdYDf3saPTJmem2ZmaoK~n4FjBADoFv7iVa~itMnTek18S1d5H0I~b0DY2yp4XQKdVfoiLyTltpVMSEc72GNlUbilhWweHEJq9JQJhZTF~6lt0py7k3kklJMHG0KQ-U8~jxi9A2SjcbYz14sNoel9amBsvONWjP5JbgORMuToRTplLxlpmETMOQ2xKqbXZe8lFIe3Ovg94Z4VvcBlFB7GAN5V7lTcFNiks45uZ5FKNbRYrJ16Kjpq3Sr-F0afh77s4qyH0zYZQyvPEgWtS-Bzv8e9MAJi63anWS~p7s8xVehm1zxMD0auZHG~uYQRir8w__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"The_Genomes_of_Two_Strains_of_Taenia_crassiceps_the_Animal_Model_for_the_Study_of_Human_Cysticercosis","translated_slug":"","page_count":12,"language":"en","content_type":"Work","summary":"Human cysticercosis by Taenia solium is the major cause of neurological illness in countries of Africa, Southeast Asia, and the Americas. Publication of four cestode genomes (T. solium, Echinococcus multilocularis, E. granulosus and Hymenolepis microstoma) in the last decade, marked the advent of novel approaches on the study of the host-parasite molecular crosstalk for cestode parasites of importance for human and animal health. Taenia crassiceps is another cestode parasite, closely related to T. solium, which has been used in numerous studies as an animal model for human cysticercosis. Therefore, characterization of the T. crassiceps genome will also contribute to the understanding of the human infection. Here, we report the genome of T. crassiceps WFU strain, reconstructed to a noncontiguous finished resolution and performed a genomic and differential expression comparison analysis against ORF strain. Both strain genomes were sequenced using Oxford Nanopore (MinION) and Illumina ...","owner":{"id":36697270,"first_name":"Julio","middle_initials":null,"last_name":"Carrero","page_name":"JulioCarrero1","domain_name":"independent","created_at":"2015-10-21T12:09:01.815-07:00","display_name":"Julio Carrero","url":"https://independent.academia.edu/JulioCarrero1"},"attachments":[{"id":110480520,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/110480520/thumbnails/1.jpg","file_name":"pdf.pdf","download_url":"https://www.academia.edu/attachments/110480520/download_file","bulk_download_file_name":"The_Genomes_of_Two_Strains_of_Taenia_cra.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/110480520/pdf-libre.pdf?1705355551=\u0026response-content-disposition=attachment%3B+filename%3DThe_Genomes_of_Two_Strains_of_Taenia_cra.pdf\u0026Expires=1743230063\u0026Signature=OR6uGi7KdO30BCljSus-0BdYDf3saPTJmem2ZmaoK~n4FjBADoFv7iVa~itMnTek18S1d5H0I~b0DY2yp4XQKdVfoiLyTltpVMSEc72GNlUbilhWweHEJq9JQJhZTF~6lt0py7k3kklJMHG0KQ-U8~jxi9A2SjcbYz14sNoel9amBsvONWjP5JbgORMuToRTplLxlpmETMOQ2xKqbXZe8lFIe3Ovg94Z4VvcBlFB7GAN5V7lTcFNiks45uZ5FKNbRYrJ16Kjpq3Sr-F0afh77s4qyH0zYZQyvPEgWtS-Bzv8e9MAJi63anWS~p7s8xVehm1zxMD0auZHG~uYQRir8w__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":176486,"name":"Genome","url":"https://www.academia.edu/Documents/in/Genome"},{"id":609113,"name":"Cestoda","url":"https://www.academia.edu/Documents/in/Cestoda"},{"id":1458519,"name":"Cysticercosis","url":"https://www.academia.edu/Documents/in/Cysticercosis"},{"id":2060319,"name":"Taenia solium","url":"https://www.academia.edu/Documents/in/Taenia_solium"}],"urls":[{"id":38604486,"url":"https://www.frontiersin.org/articles/10.3389/fcimb.2022.876839/full"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") if (false) { Aedu.setUpFigureCarousel('profile-work-113549610-figures'); } }); </script> <div class="js-work-strip profile--work_container" data-work-id="113549609"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/113549609/Potent_Anti_amoebic_Effects_of_Ibogaine_Voacangine_and_the_Root_Bark_Alkaloid_Fraction_of_Tabernaemontana_arborea"><img alt="Research paper thumbnail of Potent Anti-amoebic Effects of Ibogaine, Voacangine and the Root Bark Alkaloid Fraction of Tabernaemontana arborea" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title">Potent Anti-amoebic Effects of Ibogaine, Voacangine and the Root Bark Alkaloid Fraction of Tabernaemontana arborea</div><div class="wp-workCard_item"><span>Planta Medica</span><span>, 2022</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Plants of Tabernaemontana species have several pharmacological activities including antimicrobial...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Plants of Tabernaemontana species have several pharmacological activities including antimicrobial effects. Amoebiasis continues to be a public health problem, with increasing evidence of resistance to metronidazole. In this study, we assessed the effect of the alkaloid fraction of T. arborea root bark and the alkaloids ibogaine and voacangine on the viability and infectivity of Entamoeba histolytica trophozoites. Cultures were exposed to 0.1 – 10 µg/mL for 24, 48 and 72 h, and viability was then determined using a tetrazolium dye reduction assay and type of cellular death analyzed by flow cytometry. Results showed that the alkaloid fraction, but mainly ibogaine and voacangine alkaloids, exhibited potent dose-dependent anti-amoebic activity at 24 h post-exposure (IC50 4.5 and 8.1 µM, respectively), comparable to metronidazole (IC50 6.8 µM). However, the effect decreased after 48 and 72 h of exposure to concentrations below 10 µg/mL, suggesting that the alkaloids probably were catabol...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="113549609"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="113549609"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 113549609; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=113549609]").text(description); $(".js-view-count[data-work-id=113549609]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 113549609; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='113549609']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=113549609]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":113549609,"title":"Potent Anti-amoebic Effects of Ibogaine, Voacangine and the Root Bark Alkaloid Fraction of Tabernaemontana arborea","translated_title":"","metadata":{"abstract":"Plants of Tabernaemontana species have several pharmacological activities including antimicrobial effects. Amoebiasis continues to be a public health problem, with increasing evidence of resistance to metronidazole. In this study, we assessed the effect of the alkaloid fraction of T. arborea root bark and the alkaloids ibogaine and voacangine on the viability and infectivity of Entamoeba histolytica trophozoites. Cultures were exposed to 0.1 – 10 µg/mL for 24, 48 and 72 h, and viability was then determined using a tetrazolium dye reduction assay and type of cellular death analyzed by flow cytometry. Results showed that the alkaloid fraction, but mainly ibogaine and voacangine alkaloids, exhibited potent dose-dependent anti-amoebic activity at 24 h post-exposure (IC50 4.5 and 8.1 µM, respectively), comparable to metronidazole (IC50 6.8 µM). However, the effect decreased after 48 and 72 h of exposure to concentrations below 10 µg/mL, suggesting that the alkaloids probably were catabol...","publisher":"Georg Thieme Verlag KG","publication_date":{"day":null,"month":null,"year":2022,"errors":{}},"publication_name":"Planta Medica"},"translated_abstract":"Plants of Tabernaemontana species have several pharmacological activities including antimicrobial effects. Amoebiasis continues to be a public health problem, with increasing evidence of resistance to metronidazole. In this study, we assessed the effect of the alkaloid fraction of T. arborea root bark and the alkaloids ibogaine and voacangine on the viability and infectivity of Entamoeba histolytica trophozoites. Cultures were exposed to 0.1 – 10 µg/mL for 24, 48 and 72 h, and viability was then determined using a tetrazolium dye reduction assay and type of cellular death analyzed by flow cytometry. Results showed that the alkaloid fraction, but mainly ibogaine and voacangine alkaloids, exhibited potent dose-dependent anti-amoebic activity at 24 h post-exposure (IC50 4.5 and 8.1 µM, respectively), comparable to metronidazole (IC50 6.8 µM). However, the effect decreased after 48 and 72 h of exposure to concentrations below 10 µg/mL, suggesting that the alkaloids probably were catabol...","internal_url":"https://www.academia.edu/113549609/Potent_Anti_amoebic_Effects_of_Ibogaine_Voacangine_and_the_Root_Bark_Alkaloid_Fraction_of_Tabernaemontana_arborea","translated_internal_url":"","created_at":"2024-01-15T12:51:32.650-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":36697270,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Potent_Anti_amoebic_Effects_of_Ibogaine_Voacangine_and_the_Root_Bark_Alkaloid_Fraction_of_Tabernaemontana_arborea","translated_slug":"","page_count":null,"language":"en","content_type":"Work","summary":"Plants of Tabernaemontana species have several pharmacological activities including antimicrobial effects. Amoebiasis continues to be a public health problem, with increasing evidence of resistance to metronidazole. In this study, we assessed the effect of the alkaloid fraction of T. arborea root bark and the alkaloids ibogaine and voacangine on the viability and infectivity of Entamoeba histolytica trophozoites. Cultures were exposed to 0.1 – 10 µg/mL for 24, 48 and 72 h, and viability was then determined using a tetrazolium dye reduction assay and type of cellular death analyzed by flow cytometry. Results showed that the alkaloid fraction, but mainly ibogaine and voacangine alkaloids, exhibited potent dose-dependent anti-amoebic activity at 24 h post-exposure (IC50 4.5 and 8.1 µM, respectively), comparable to metronidazole (IC50 6.8 µM). However, the effect decreased after 48 and 72 h of exposure to concentrations below 10 µg/mL, suggesting that the alkaloids probably were catabol...","owner":{"id":36697270,"first_name":"Julio","middle_initials":null,"last_name":"Carrero","page_name":"JulioCarrero1","domain_name":"independent","created_at":"2015-10-21T12:09:01.815-07:00","display_name":"Julio Carrero","url":"https://independent.academia.edu/JulioCarrero1"},"attachments":[],"research_interests":[{"id":4083,"name":"Complementary and Alternative Medicine","url":"https://www.academia.edu/Documents/in/Complementary_and_Alternative_Medicine"},{"id":4086,"name":"Traditional Medicine","url":"https://www.academia.edu/Documents/in/Traditional_Medicine"},{"id":5541,"name":"Plant Biology","url":"https://www.academia.edu/Documents/in/Plant_Biology"},{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":133215,"name":"Amoebiasis","url":"https://www.academia.edu/Documents/in/Amoebiasis"},{"id":571342,"name":"Entamoeba histolytica","url":"https://www.academia.edu/Documents/in/Entamoeba_histolytica"},{"id":597910,"name":"Alkaloid","url":"https://www.academia.edu/Documents/in/Alkaloid"},{"id":3789884,"name":"Pharmacology and pharmaceutical sciences","url":"https://www.academia.edu/Documents/in/Pharmacology_and_pharmaceutical_sciences"}],"urls":[{"id":38604485,"url":"http://www.thieme-connect.de/products/ejournals/pdf/10.1055/a-1809-1157.pdf"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") if (false) { Aedu.setUpFigureCarousel('profile-work-113549609-figures'); } }); </script> <div class="js-work-strip profile--work_container" data-work-id="113549608"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/113549608/Oxidative_Stress_and_Heat_Stress_in_Experimental_Amoebic_Liver_Abscess"><img alt="Research paper thumbnail of Oxidative Stress and Heat Stress in Experimental Amoebic Liver Abscess" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title">Oxidative Stress and Heat Stress in Experimental Amoebic Liver Abscess</div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Several species belonging to the Genus Entamoeba can colonize the mouth or the human gut; however...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Several species belonging to the Genus Entamoeba can colonize the mouth or the human gut; however, only Entamoeba histolytica is pathogenic to the host, causing amoebiasis. This illness leads to one hundred thousand deaths per year worldwide, affecting mainly underdeveloped countries in areas with poor sanitary conditions. Throughout its life cycle or during the invasion of human tissues, the parasite is constantly subjected to stress conditions. In in vitro culture, this microaerophilic parasite can tolerate up to 50 μM oxygen concentrations; however, during invasion the parasite has to cope with the higher oxygen content found in the blood and well perfused tissues (60–130 μM) and with reactive oxygen and nitrogen species (ROS and NOS, respectively) derived from both the host (as a first line of defense against the infection) and from the oxygen and nitrogen detoxification systems within the parasite. Furthermore, notwithstanding that host body temperatures (36.5–39 ℃) observed du...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="113549608"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="113549608"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 113549608; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=113549608]").text(description); $(".js-view-count[data-work-id=113549608]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 113549608; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='113549608']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=113549608]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":113549608,"title":"Oxidative Stress and Heat Stress in Experimental Amoebic Liver Abscess","translated_title":"","metadata":{"abstract":"Several species belonging to the Genus Entamoeba can colonize the mouth or the human gut; however, only Entamoeba histolytica is pathogenic to the host, causing amoebiasis. This illness leads to one hundred thousand deaths per year worldwide, affecting mainly underdeveloped countries in areas with poor sanitary conditions. Throughout its life cycle or during the invasion of human tissues, the parasite is constantly subjected to stress conditions. In in vitro culture, this microaerophilic parasite can tolerate up to 50 μM oxygen concentrations; however, during invasion the parasite has to cope with the higher oxygen content found in the blood and well perfused tissues (60–130 μM) and with reactive oxygen and nitrogen species (ROS and NOS, respectively) derived from both the host (as a first line of defense against the infection) and from the oxygen and nitrogen detoxification systems within the parasite. Furthermore, notwithstanding that host body temperatures (36.5–39 ℃) observed du...","publication_date":{"day":null,"month":null,"year":2020,"errors":{}}},"translated_abstract":"Several species belonging to the Genus Entamoeba can colonize the mouth or the human gut; however, only Entamoeba histolytica is pathogenic to the host, causing amoebiasis. This illness leads to one hundred thousand deaths per year worldwide, affecting mainly underdeveloped countries in areas with poor sanitary conditions. Throughout its life cycle or during the invasion of human tissues, the parasite is constantly subjected to stress conditions. In in vitro culture, this microaerophilic parasite can tolerate up to 50 μM oxygen concentrations; however, during invasion the parasite has to cope with the higher oxygen content found in the blood and well perfused tissues (60–130 μM) and with reactive oxygen and nitrogen species (ROS and NOS, respectively) derived from both the host (as a first line of defense against the infection) and from the oxygen and nitrogen detoxification systems within the parasite. Furthermore, notwithstanding that host body temperatures (36.5–39 ℃) observed du...","internal_url":"https://www.academia.edu/113549608/Oxidative_Stress_and_Heat_Stress_in_Experimental_Amoebic_Liver_Abscess","translated_internal_url":"","created_at":"2024-01-15T12:51:32.540-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":36697270,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Oxidative_Stress_and_Heat_Stress_in_Experimental_Amoebic_Liver_Abscess","translated_slug":"","page_count":null,"language":"en","content_type":"Work","summary":"Several species belonging to the Genus Entamoeba can colonize the mouth or the human gut; however, only Entamoeba histolytica is pathogenic to the host, causing amoebiasis. This illness leads to one hundred thousand deaths per year worldwide, affecting mainly underdeveloped countries in areas with poor sanitary conditions. Throughout its life cycle or during the invasion of human tissues, the parasite is constantly subjected to stress conditions. In in vitro culture, this microaerophilic parasite can tolerate up to 50 μM oxygen concentrations; however, during invasion the parasite has to cope with the higher oxygen content found in the blood and well perfused tissues (60–130 μM) and with reactive oxygen and nitrogen species (ROS and NOS, respectively) derived from both the host (as a first line of defense against the infection) and from the oxygen and nitrogen detoxification systems within the parasite. Furthermore, notwithstanding that host body temperatures (36.5–39 ℃) observed du...","owner":{"id":36697270,"first_name":"Julio","middle_initials":null,"last_name":"Carrero","page_name":"JulioCarrero1","domain_name":"independent","created_at":"2015-10-21T12:09:01.815-07:00","display_name":"Julio Carrero","url":"https://independent.academia.edu/JulioCarrero1"},"attachments":[],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology"},{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":14292,"name":"Oxidative Stress","url":"https://www.academia.edu/Documents/in/Oxidative_Stress"},{"id":82978,"name":"Reactive Oxygen Species","url":"https://www.academia.edu/Documents/in/Reactive_Oxygen_Species"},{"id":383248,"name":"Heat stress","url":"https://www.academia.edu/Documents/in/Heat_stress"},{"id":571342,"name":"Entamoeba histolytica","url":"https://www.academia.edu/Documents/in/Entamoeba_histolytica"},{"id":892623,"name":"Amoebic Liver Abscess","url":"https://www.academia.edu/Documents/in/Amoebic_Liver_Abscess"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") if (false) { Aedu.setUpFigureCarousel('profile-work-113549608-figures'); } }); </script> <div class="js-work-strip profile--work_container" data-work-id="113549607"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/113549607/Role_of_Extracellular_Traps_Promoted_by_Intestinal_Parasites_Relationship_with_Virulence"><img alt="Research paper thumbnail of Role of Extracellular Traps Promoted by Intestinal Parasites. Relationship with Virulence" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title">Role of Extracellular Traps Promoted by Intestinal Parasites. Relationship with Virulence</div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">NETosis is a form of programmed cell death in neutrophils characterized by the release of extrace...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">NETosis is a form of programmed cell death in neutrophils characterized by the release of extracellular DNA traps (NET) composed of DNA associated with histones, granule enzymes and antimicrobial peptides. This mechanism of innate immunity has been linked to cell defense against different pathogens. In case of bacteria, NETosis has been directly associated with virulence, suggesting that its formation could depend on the pathogen’s ability to cause tissue damage. Moreover, NET formation can contribute to tissue destruction by promoting local inflammation and coagulation when exacerbated.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="113549607"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="113549607"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 113549607; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=113549607]").text(description); $(".js-view-count[data-work-id=113549607]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 113549607; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='113549607']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=113549607]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":113549607,"title":"Role of Extracellular Traps Promoted by Intestinal Parasites. Relationship with Virulence","translated_title":"","metadata":{"abstract":"NETosis is a form of programmed cell death in neutrophils characterized by the release of extracellular DNA traps (NET) composed of DNA associated with histones, granule enzymes and antimicrobial peptides. This mechanism of innate immunity has been linked to cell defense against different pathogens. In case of bacteria, NETosis has been directly associated with virulence, suggesting that its formation could depend on the pathogen’s ability to cause tissue damage. Moreover, NET formation can contribute to tissue destruction by promoting local inflammation and coagulation when exacerbated.","publication_date":{"day":null,"month":null,"year":2020,"errors":{}}},"translated_abstract":"NETosis is a form of programmed cell death in neutrophils characterized by the release of extracellular DNA traps (NET) composed of DNA associated with histones, granule enzymes and antimicrobial peptides. This mechanism of innate immunity has been linked to cell defense against different pathogens. In case of bacteria, NETosis has been directly associated with virulence, suggesting that its formation could depend on the pathogen’s ability to cause tissue damage. Moreover, NET formation can contribute to tissue destruction by promoting local inflammation and coagulation when exacerbated.","internal_url":"https://www.academia.edu/113549607/Role_of_Extracellular_Traps_Promoted_by_Intestinal_Parasites_Relationship_with_Virulence","translated_internal_url":"","created_at":"2024-01-15T12:51:32.454-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":36697270,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Role_of_Extracellular_Traps_Promoted_by_Intestinal_Parasites_Relationship_with_Virulence","translated_slug":"","page_count":null,"language":"en","content_type":"Work","summary":"NETosis is a form of programmed cell death in neutrophils characterized by the release of extracellular DNA traps (NET) composed of DNA associated with histones, granule enzymes and antimicrobial peptides. This mechanism of innate immunity has been linked to cell defense against different pathogens. In case of bacteria, NETosis has been directly associated with virulence, suggesting that its formation could depend on the pathogen’s ability to cause tissue damage. Moreover, NET formation can contribute to tissue destruction by promoting local inflammation and coagulation when exacerbated.","owner":{"id":36697270,"first_name":"Julio","middle_initials":null,"last_name":"Carrero","page_name":"JulioCarrero1","domain_name":"independent","created_at":"2015-10-21T12:09:01.815-07:00","display_name":"Julio Carrero","url":"https://independent.academia.edu/JulioCarrero1"},"attachments":[],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology"},{"id":523,"name":"Chemistry","url":"https://www.academia.edu/Documents/in/Chemistry"},{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":9334,"name":"Inflammation","url":"https://www.academia.edu/Documents/in/Inflammation"},{"id":52873,"name":"Virulence","url":"https://www.academia.edu/Documents/in/Virulence"},{"id":164268,"name":"Pathogen","url":"https://www.academia.edu/Documents/in/Pathogen"},{"id":175490,"name":"Programmed cell death","url":"https://www.academia.edu/Documents/in/Programmed_cell_death"},{"id":1274621,"name":"Extracellular","url":"https://www.academia.edu/Documents/in/Extracellular"},{"id":1938317,"name":"Innate Immune System","url":"https://www.academia.edu/Documents/in/Innate_Immune_System"},{"id":2207504,"name":"Neutrophil extracellular traps","url":"https://www.academia.edu/Documents/in/Neutrophil_extracellular_traps"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") if (false) { Aedu.setUpFigureCarousel('profile-work-113549607-figures'); } }); </script> <div class="js-work-strip profile--work_container" data-work-id="113549606"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/113549606/Interferon_gamma_activated_macrophages_infected_with_Burkholderia_cenocepacia_process_and_present_bacterial_antigens_to_T_cells_by_class_I_and_II_major_histocompatibility_complex_molecules"><img alt="Research paper thumbnail of Interferon-gamma-activated macrophages infected with Burkholderia cenocepacia process and present bacterial antigens to T-cells by class I and II major histocompatibility complex molecules" class="work-thumbnail" src="https://attachments.academia-assets.com/110480454/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/113549606/Interferon_gamma_activated_macrophages_infected_with_Burkholderia_cenocepacia_process_and_present_bacterial_antigens_to_T_cells_by_class_I_and_II_major_histocompatibility_complex_molecules">Interferon-gamma-activated macrophages infected with Burkholderia cenocepacia process and present bacterial antigens to T-cells by class I and II major histocompatibility complex molecules</a></div><div class="wp-workCard_item"><span>Emerging Microbes &amp; Infections</span><span>, 2020</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Burkholderia cenocepacia is an emerging opportunistic pathogen for people with cystic fibrosis an...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Burkholderia cenocepacia is an emerging opportunistic pathogen for people with cystic fibrosis and chronic granulomatous disease. Intracellular survival in macrophages within a membrane-bound vacuole (BcCV) that delays acidification and maturation into lysosomes is a hallmark of B. cenocepacia infection. Intracellular B. cenocepacia induce an inflammatory response leading to macrophage cell death by pyroptosis through the secretion of a bacterial deamidase that results in the activation of the pyrin inflammasome. However, how or whether infected macrophages can process and present B. cenocepacia antigens to activate T-cells has not been explored. Engulfed bacterial protein antigens are cleaved into small peptides in the late endosomal major histocompatibility class II complex (MHC) compartment (MIIC). Here, we demonstrate that BcCVs and MIICs have overlapping features and that interferongamma-activated macrophages infected with B. cenocepacia can process bacterial antigens for presentation by class II MHC molecules to CD4 + T-cells and by class I MHC molecules to CD8 + T-cells. Infected macrophages also release processed bacterial peptides into the extracellular medium, stabilizing empty class I MHC molecules of bystander cells. Together, we conclude that BcCVs acquire MIIC characteristics, supporting the notion that macrophages infected with B. cenocepacia contribute to establishing an adaptive immune response against the pathogen.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="cb12ed5de77311187a3ef402f0eaa270" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":110480454,"asset_id":113549606,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/110480454/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="113549606"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="113549606"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 113549606; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=113549606]").text(description); $(".js-view-count[data-work-id=113549606]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 113549606; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='113549606']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "cb12ed5de77311187a3ef402f0eaa270" } } $('.js-work-strip[data-work-id=113549606]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":113549606,"title":"Interferon-gamma-activated macrophages infected with Burkholderia cenocepacia process and present bacterial antigens to T-cells by class I and II major histocompatibility complex molecules","translated_title":"","metadata":{"publisher":"Informa UK Limited","grobid_abstract":"Burkholderia cenocepacia is an emerging opportunistic pathogen for people with cystic fibrosis and chronic granulomatous disease. Intracellular survival in macrophages within a membrane-bound vacuole (BcCV) that delays acidification and maturation into lysosomes is a hallmark of B. cenocepacia infection. Intracellular B. cenocepacia induce an inflammatory response leading to macrophage cell death by pyroptosis through the secretion of a bacterial deamidase that results in the activation of the pyrin inflammasome. However, how or whether infected macrophages can process and present B. cenocepacia antigens to activate T-cells has not been explored. Engulfed bacterial protein antigens are cleaved into small peptides in the late endosomal major histocompatibility class II complex (MHC) compartment (MIIC). Here, we demonstrate that BcCVs and MIICs have overlapping features and that interferongamma-activated macrophages infected with B. cenocepacia can process bacterial antigens for presentation by class II MHC molecules to CD4 + T-cells and by class I MHC molecules to CD8 + T-cells. Infected macrophages also release processed bacterial peptides into the extracellular medium, stabilizing empty class I MHC molecules of bystander cells. Together, we conclude that BcCVs acquire MIIC characteristics, supporting the notion that macrophages infected with B. cenocepacia contribute to establishing an adaptive immune response against the pathogen.","publication_date":{"day":null,"month":null,"year":2020,"errors":{}},"publication_name":"Emerging Microbes \u0026amp; Infections","grobid_abstract_attachment_id":110480455},"translated_abstract":null,"internal_url":"https://www.academia.edu/113549606/Interferon_gamma_activated_macrophages_infected_with_Burkholderia_cenocepacia_process_and_present_bacterial_antigens_to_T_cells_by_class_I_and_II_major_histocompatibility_complex_molecules","translated_internal_url":"","created_at":"2024-01-15T12:51:32.322-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":36697270,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":110480454,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/110480454/thumbnails/1.jpg","file_name":"22221751.2020.pdf","download_url":"https://www.academia.edu/attachments/110480454/download_file","bulk_download_file_name":"Interferon_gamma_activated_macrophages_i.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/110480454/22221751.2020-libre.pdf?1705355561=\u0026response-content-disposition=attachment%3B+filename%3DInterferon_gamma_activated_macrophages_i.pdf\u0026Expires=1743230063\u0026Signature=d0ErqDPfM6vRkbSzfnn2wBEUGpmjkbiiaGKzt1sKCYsH6Ly9Wq9hX~jL2skDXShKjLdQjb2BczOE7Pv~HFCg1~MczLcQ4oMJGYHO-2s-u6atM-I6zS7vxGOVIxdiRBFvdmR9A92IelTyCsls97znKgY9b9zPJgDxtO9p6aiE7Vs6mQI3HNu31wooG~zTRkNR1Q5woef07Q1jTJjxYdEvvKnsRU2Z6cOO0nOYYZGdW9CoifKldGS57FBOlTfX-RCeAZEYq-ZP2hZrD6jRF~eOHGAWvTNtCu-Y2MsR0Pn-kas4-RzAhcFF4IASlXmWxjIV7plNbRE~MX1s94eqEhSKaA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Interferon_gamma_activated_macrophages_infected_with_Burkholderia_cenocepacia_process_and_present_bacterial_antigens_to_T_cells_by_class_I_and_II_major_histocompatibility_complex_molecules","translated_slug":"","page_count":14,"language":"en","content_type":"Work","summary":"Burkholderia cenocepacia is an emerging opportunistic pathogen for people with cystic fibrosis and chronic granulomatous disease. Intracellular survival in macrophages within a membrane-bound vacuole (BcCV) that delays acidification and maturation into lysosomes is a hallmark of B. cenocepacia infection. Intracellular B. cenocepacia induce an inflammatory response leading to macrophage cell death by pyroptosis through the secretion of a bacterial deamidase that results in the activation of the pyrin inflammasome. However, how or whether infected macrophages can process and present B. cenocepacia antigens to activate T-cells has not been explored. Engulfed bacterial protein antigens are cleaved into small peptides in the late endosomal major histocompatibility class II complex (MHC) compartment (MIIC). Here, we demonstrate that BcCVs and MIICs have overlapping features and that interferongamma-activated macrophages infected with B. cenocepacia can process bacterial antigens for presentation by class II MHC molecules to CD4 + T-cells and by class I MHC molecules to CD8 + T-cells. Infected macrophages also release processed bacterial peptides into the extracellular medium, stabilizing empty class I MHC molecules of bystander cells. Together, we conclude that BcCVs acquire MIIC characteristics, supporting the notion that macrophages infected with B. cenocepacia contribute to establishing an adaptive immune response against the pathogen.","owner":{"id":36697270,"first_name":"Julio","middle_initials":null,"last_name":"Carrero","page_name":"JulioCarrero1","domain_name":"independent","created_at":"2015-10-21T12:09:01.815-07:00","display_name":"Julio Carrero","url":"https://independent.academia.edu/JulioCarrero1"},"attachments":[{"id":110480454,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/110480454/thumbnails/1.jpg","file_name":"22221751.2020.pdf","download_url":"https://www.academia.edu/attachments/110480454/download_file","bulk_download_file_name":"Interferon_gamma_activated_macrophages_i.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/110480454/22221751.2020-libre.pdf?1705355561=\u0026response-content-disposition=attachment%3B+filename%3DInterferon_gamma_activated_macrophages_i.pdf\u0026Expires=1743230063\u0026Signature=d0ErqDPfM6vRkbSzfnn2wBEUGpmjkbiiaGKzt1sKCYsH6Ly9Wq9hX~jL2skDXShKjLdQjb2BczOE7Pv~HFCg1~MczLcQ4oMJGYHO-2s-u6atM-I6zS7vxGOVIxdiRBFvdmR9A92IelTyCsls97znKgY9b9zPJgDxtO9p6aiE7Vs6mQI3HNu31wooG~zTRkNR1Q5woef07Q1jTJjxYdEvvKnsRU2Z6cOO0nOYYZGdW9CoifKldGS57FBOlTfX-RCeAZEYq-ZP2hZrD6jRF~eOHGAWvTNtCu-Y2MsR0Pn-kas4-RzAhcFF4IASlXmWxjIV7plNbRE~MX1s94eqEhSKaA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"},{"id":110480455,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/110480455/thumbnails/1.jpg","file_name":"22221751.2020.pdf","download_url":"https://www.academia.edu/attachments/110480455/download_file","bulk_download_file_name":"Interferon_gamma_activated_macrophages_i.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/110480455/22221751.2020-libre.pdf?1705355557=\u0026response-content-disposition=attachment%3B+filename%3DInterferon_gamma_activated_macrophages_i.pdf\u0026Expires=1743230063\u0026Signature=M5nXhSHXxnZjFXHTbc81TqHHxr2XFdZsWd-iPJYX45h7WBYfvJqFQZzbOa7P5y86FWrcNTU0eG2qWOgl6ncyu7xaLcbjDEow9jWPRL6A2jU-W4v9Tlb83syYsVxF9yiHP-7owV9JoCxabgULjxg38iIWslpCgliX6wO7XFhUXMxuDRij1vNQEl7dXpBBAdF2Pxo0vlJN3mnX8xMvjJavabIofASbMF7i505CUGeE68qA2Rqx39CPa0zflpOQeg0i0mFw3-5nixOzHLc6uQDevesWD5g27iW3QzHqHhKn8A5I6cuJyNwTiiq~1CqJr~5yH5Vr1ETGqZn5NHC5s9ne3g__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology"},{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":135250,"name":"Antigen Presentation","url":"https://www.academia.edu/Documents/in/Antigen_Presentation"},{"id":251290,"name":"MHC class II","url":"https://www.academia.edu/Documents/in/MHC_class_II"},{"id":435727,"name":"Major histocompatibility complex","url":"https://www.academia.edu/Documents/in/Major_histocompatibility_complex"}],"urls":[{"id":38604484,"url":"https://www.tandfonline.com/doi/pdf/10.1080/22221751.2020.1818632"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") if (false) { Aedu.setUpFigureCarousel('profile-work-113549606-figures'); } }); </script> </div><div class="profile--tab_content_container js-tab-pane tab-pane" data-section-id="3809780" id="papers"><div class="js-work-strip profile--work_container" data-work-id="127950220"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/127950220/Characterization_of_Tau95_led_to_the_identification_of_a_four_subunit_TFIIIC_complex_in_trypanosomatid_parasites"><img alt="Research paper thumbnail of Characterization of Tau95 led to the identification of a four-subunit TFIIIC complex in trypanosomatid parasites" class="work-thumbnail" src="https://attachments.academia-assets.com/121605894/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/127950220/Characterization_of_Tau95_led_to_the_identification_of_a_four_subunit_TFIIIC_complex_in_trypanosomatid_parasites">Characterization of Tau95 led to the identification of a four-subunit TFIIIC complex in trypanosomatid parasites</a></div><div class="wp-workCard_item"><span>Applied Microbiology and Biotechnology</span><span>, Jan 9, 2024</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">RNA polymerase III (RNAP III) synthetizes small essential non-coding RNA molecules such as tRNAs ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">RNA polymerase III (RNAP III) synthetizes small essential non-coding RNA molecules such as tRNAs and 5S rRNA. In yeast and vertebrates, RNAP III needs general transcription factors TFIIIA, TFIIIB, and TFIIIC to initiate transcription. TFIIIC, composed of six subunits, binds to internal promoter elements in RNAP III-dependent genes. Limited information is available about RNAP III transcription in the trypanosomatid protozoa Trypanosoma brucei and Leishmania major, which diverged early from the eukaryotic lineage. Analyses of the first published draft of the trypanosomatid genome sequences failed to recognize orthologs of any of the TFIIIC subunits, suggesting that this transcription factor is absent in these parasites. However, a putative TFIIIC subunit was recently annotated in the databases. Here we characterize this subunit in T. brucei and L. major and demonstrate that it corresponds to Tau95. In silico analyses showed that both proteins possess the typical Tau95 sequences: the DNA binding region and the dimerization domain. As anticipated for a transcription factor, Tau95 localized to the nucleus in insect forms of both parasites. Chromatin immunoprecipitation (ChIP) assays demonstrated that Tau95 binds to tRNA and U2 snRNA genes in T. brucei. Remarkably, by performing tandem affinity purifications we identified orthologs of TFIIIC subunits Tau55, Tau131, and Tau138 in T. brucei and L. major. Thus, contrary to what was assumed, trypanosomatid parasites do possess a TFIIIC complex. Other putative interacting partners of Tau95 were identified in T. brucei and L. major. • A four-subunit TFIIIC complex is present in T. brucei and L. major • TbTau95 associates with tRNA and U2 snRNA genes • Putative interacting partners of Tau95 might include some RNAP II regulators</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="bbcd90dbbe60229b758d4595fd2ae867" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":121605894,"asset_id":127950220,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/121605894/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="127950220"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="127950220"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 127950220; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=127950220]").text(description); $(".js-view-count[data-work-id=127950220]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 127950220; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='127950220']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "bbcd90dbbe60229b758d4595fd2ae867" } } $('.js-work-strip[data-work-id=127950220]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":127950220,"title":"Characterization of Tau95 led to the identification of a four-subunit TFIIIC complex in trypanosomatid parasites","translated_title":"","metadata":{"publisher":"Springer Science+Business Media","grobid_abstract":"RNA polymerase III (RNAP III) synthetizes small essential non-coding RNA molecules such as tRNAs and 5S rRNA. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") if (false) { Aedu.setUpFigureCarousel('profile-work-127950220-figures'); } }); </script> <div class="js-work-strip profile--work_container" data-work-id="113549624"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/113549624/IDENTIFICACI%C3%93N_DE_ANT%C3%8DGENOS_ESPEC%C3%8DFICOS_PARA_EL_DESARROLLO_DE_UNA_PRUEBA_DIAGN%C3%93STICA_PARA_LA_CISTICERCOSIS_POR_Taenia_solium"><img alt="Research paper thumbnail of IDENTIFICACIÓN DE ANTÍGENOS ESPECÍFICOS PARA EL DESARROLLO DE UNA PRUEBA DIAGNÓSTICA PARA LA CISTICERCOSIS POR Taenia solium" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title">IDENTIFICACIÓN DE ANTÍGENOS ESPECÍFICOS PARA EL DESARROLLO DE UNA PRUEBA DIAGNÓSTICA PARA LA CISTICERCOSIS POR Taenia solium</div><div class="wp-workCard_item"><span>8th Cuban Congress on Microbiology and Parasitology, 5th National Congress on Tropical Medicine and 5th International Symposium on HIV/aids infection in Cuba</span><span>, Sep 9, 2014</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="113549624"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="113549624"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 113549624; 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Lactoferricins (Lfcins) are peptides derived from the N-terminus of Lf. Lf avoids the iron availability to parasites in the body fluids due to its high avidity for iron, maintaining together with transferrin the free-iron concentration in about 10 −18 M, which is too low to support the pathogenic invader survival. Intestinal parasitic diseases affect people worldwide, mainly in developing countries with poor hygienic conditions; for example, parasites such as Entamoeba histolytica, Giardia intestinalis, and Cryptosporidium parvum infect the human intestine when are orally ingested as cysts. Human and bovine Lf have been found parasiticidal in experiments in vitro and in animal models. Interestingly, Lf synergizes with metronidazole, the main drug used against E. histolytica and G. intestinalis. The aim of this chapter is to show the benefits of using Lf and Lfcins against intestinal parasitic diseases.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="5c0a9bf2e287182b8221aff73600919c" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":110480514,"asset_id":113549622,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/110480514/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="113549622"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="113549622"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 113549622; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=113549622]").text(description); $(".js-view-count[data-work-id=113549622]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 113549622; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='113549622']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "5c0a9bf2e287182b8221aff73600919c" } } $('.js-work-strip[data-work-id=113549622]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":113549622,"title":"Lactoferrin in the battle against intestinal parasites","translated_title":"","metadata":{"ai_title_tag":"Lactoferrin's Role Against Intestinal Parasites","grobid_abstract":"Lactoferrin is an iron-binding glycoprotein of the innate immune system, which is present in some mammalian fluids and secreted into the mucosae; it is also produced by the secondary granules of the polymorphonuclear neutrophils and secreted at infection sites. 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The aim of this chapter is to show the benefits of using Lf and Lfcins against intestinal parasitic diseases.","owner":{"id":36697270,"first_name":"Julio","middle_initials":null,"last_name":"Carrero","page_name":"JulioCarrero1","domain_name":"independent","created_at":"2015-10-21T12:09:01.815-07:00","display_name":"Julio Carrero","url":"https://independent.academia.edu/JulioCarrero1"},"attachments":[{"id":110480514,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/110480514/thumbnails/1.jpg","file_name":"53563.pdf","download_url":"https://www.academia.edu/attachments/110480514/download_file","bulk_download_file_name":"Lactoferrin_in_the_battle_against_intest.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/110480514/53563-libre.pdf?1705355555=\u0026response-content-disposition=attachment%3B+filename%3DLactoferrin_in_the_battle_against_intest.pdf\u0026Expires=1743230062\u0026Signature=PZMHo-Y09zkJAPyHtvO0DqL62ngoTjuezHL6e8wkMj6ARcWVKg8Hsjno2Ftc8qvPmqItNptuRf8SQqxR5diKdwr81pSkGpkXVwYxm4eQjme-aW5eWLs3wbbkUJQeUxyySlBwmgTZkDnBA~LgsGISDxM3RUe5IRuwO99ahlZskG5DbCmE1WW8MsDr85Qg4Gnwkhza39cjmkzw4eb4t-LiKydbk9naTVLS7GN2qvCRvaT7SPPu3mdTEOfsovWhkIM5F1EKBigNmhecurFrbMPzpwQzM1Sgw-ZJKpsF~55PWe0Hd3o6LFzZfHE1gfuzyNXXplqQQJ~B1911gcb3zdFcwA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":2636453,"name":"Lactoferrin","url":"https://www.academia.edu/Documents/in/Lactoferrin"},{"id":3042534,"name":"battle","url":"https://www.academia.edu/Documents/in/battle"}],"urls":[{"id":38604498,"url":"https://www.intechopen.com/books//lactoferrin-in-the-battle-against-intestinal-parasites"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") if (false) { Aedu.setUpFigureCarousel('profile-work-113549622-figures'); } }); </script> <div class="js-work-strip profile--work_container" data-work-id="113549621"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/113549621/Lactoferrin_in_the_Battle_against_Intestinal_Parasites_A_Review"><img alt="Research paper thumbnail of Lactoferrin in the Battle against Intestinal Parasites: A Review" class="work-thumbnail" src="https://attachments.academia-assets.com/110480516/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/113549621/Lactoferrin_in_the_Battle_against_Intestinal_Parasites_A_Review">Lactoferrin in the Battle against Intestinal Parasites: A Review</a></div><div class="wp-workCard_item"><span>InTech eBooks</span><span>, Jul 12, 2017</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Lactoferrin is an iron-binding glycoprotein of the innate immune system, which is present in some...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Lactoferrin is an iron-binding glycoprotein of the innate immune system, which is present in some mammalian fluids and secreted into the mucosae; it is also produced by the secondary granules of the polymorphonuclear neutrophils and secreted at infection sites. Lactoferricins (Lfcins) are peptides derived from the N-terminus of Lf. Lf avoids the iron availability to parasites in the body fluids due to its high avidity for iron, maintaining together with transferrin the free-iron concentration in about 10 −18 M, which is too low to support the pathogenic invader survival. Intestinal parasitic diseases affect people worldwide, mainly in developing countries with poor hygienic conditions; for example, parasites such as Entamoeba histolytica, Giardia intestinalis, and Cryptosporidium parvum infect the human intestine when are orally ingested as cysts. Human and bovine Lf have been found parasiticidal in experiments in vitro and in animal models. Interestingly, Lf synergizes with metronidazole, the main drug used against E. histolytica and G. intestinalis. The aim of this chapter is to show the benefits of using Lf and Lfcins against intestinal parasitic diseases.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="c7a3d544d890578d87c9806b6fe36604" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":110480516,"asset_id":113549621,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/110480516/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="113549621"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="113549621"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 113549621; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=113549621]").text(description); $(".js-view-count[data-work-id=113549621]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 113549621; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='113549621']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "c7a3d544d890578d87c9806b6fe36604" } } $('.js-work-strip[data-work-id=113549621]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":113549621,"title":"Lactoferrin in the Battle against Intestinal Parasites: A Review","translated_title":"","metadata":{"publisher":"InTech","grobid_abstract":"Lactoferrin is an iron-binding glycoprotein of the innate immune system, which is present in some mammalian fluids and secreted into the mucosae; it is also produced by the secondary granules of the polymorphonuclear neutrophils and secreted at infection sites. Lactoferricins (Lfcins) are peptides derived from the N-terminus of Lf. Lf avoids the iron availability to parasites in the body fluids due to its high avidity for iron, maintaining together with transferrin the free-iron concentration in about 10 −18 M, which is too low to support the pathogenic invader survival. Intestinal parasitic diseases affect people worldwide, mainly in developing countries with poor hygienic conditions; for example, parasites such as Entamoeba histolytica, Giardia intestinalis, and Cryptosporidium parvum infect the human intestine when are orally ingested as cysts. Human and bovine Lf have been found parasiticidal in experiments in vitro and in animal models. Interestingly, Lf synergizes with metronidazole, the main drug used against E. histolytica and G. intestinalis. The aim of this chapter is to show the benefits of using Lf and Lfcins against intestinal parasitic diseases.","publication_date":{"day":12,"month":7,"year":2017,"errors":{}},"publication_name":"InTech eBooks","grobid_abstract_attachment_id":110480516},"translated_abstract":null,"internal_url":"https://www.academia.edu/113549621/Lactoferrin_in_the_Battle_against_Intestinal_Parasites_A_Review","translated_internal_url":"","created_at":"2024-01-15T12:51:34.370-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":36697270,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":110480516,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/110480516/thumbnails/1.jpg","file_name":"53563.pdf","download_url":"https://www.academia.edu/attachments/110480516/download_file","bulk_download_file_name":"Lactoferrin_in_the_Battle_against_Intest.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/110480516/53563-libre.pdf?1705355553=\u0026response-content-disposition=attachment%3B+filename%3DLactoferrin_in_the_Battle_against_Intest.pdf\u0026Expires=1743230062\u0026Signature=bohsgEXNf3mZioQzIbe9ga~YGhsjTCxAqp9aRxmZ1mKThebe68vuoRbaLrXz4eoWFcInQ1J1EvRcvJu9aw7W9-n6Yxq8dSrI~BLWsfuvCoqaNij6Bf1O59XaQH7Sk6oCL98faGI6w36LOBxmxSiEUbDWbYTDp5-lkLpG6XkeQnD4PojGRDFQ-lSFKvCGBNa9TXb8F5US0G2gwItVyUcu-NWN0GNDSY65CGDphWffLYSwc5yQQO6wruYkNpp3YPgBfdqyd0brGs4inoEcrHqiB6m8JFQfp8g6vRn87vDp1EPS8qvetf18Djgr6QmsjYsNpxSU~6af5uKJZwa2ERWR0w__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Lactoferrin_in_the_Battle_against_Intestinal_Parasites_A_Review","translated_slug":"","page_count":29,"language":"en","content_type":"Work","summary":"Lactoferrin is an iron-binding glycoprotein of the innate immune system, which is present in some mammalian fluids and secreted into the mucosae; it is also produced by the secondary granules of the polymorphonuclear neutrophils and secreted at infection sites. Lactoferricins (Lfcins) are peptides derived from the N-terminus of Lf. Lf avoids the iron availability to parasites in the body fluids due to its high avidity for iron, maintaining together with transferrin the free-iron concentration in about 10 −18 M, which is too low to support the pathogenic invader survival. Intestinal parasitic diseases affect people worldwide, mainly in developing countries with poor hygienic conditions; for example, parasites such as Entamoeba histolytica, Giardia intestinalis, and Cryptosporidium parvum infect the human intestine when are orally ingested as cysts. Human and bovine Lf have been found parasiticidal in experiments in vitro and in animal models. Interestingly, Lf synergizes with metronidazole, the main drug used against E. histolytica and G. intestinalis. The aim of this chapter is to show the benefits of using Lf and Lfcins against intestinal parasitic diseases.","owner":{"id":36697270,"first_name":"Julio","middle_initials":null,"last_name":"Carrero","page_name":"JulioCarrero1","domain_name":"independent","created_at":"2015-10-21T12:09:01.815-07:00","display_name":"Julio Carrero","url":"https://independent.academia.edu/JulioCarrero1"},"attachments":[{"id":110480516,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/110480516/thumbnails/1.jpg","file_name":"53563.pdf","download_url":"https://www.academia.edu/attachments/110480516/download_file","bulk_download_file_name":"Lactoferrin_in_the_Battle_against_Intest.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/110480516/53563-libre.pdf?1705355553=\u0026response-content-disposition=attachment%3B+filename%3DLactoferrin_in_the_Battle_against_Intest.pdf\u0026Expires=1743230062\u0026Signature=bohsgEXNf3mZioQzIbe9ga~YGhsjTCxAqp9aRxmZ1mKThebe68vuoRbaLrXz4eoWFcInQ1J1EvRcvJu9aw7W9-n6Yxq8dSrI~BLWsfuvCoqaNij6Bf1O59XaQH7Sk6oCL98faGI6w36LOBxmxSiEUbDWbYTDp5-lkLpG6XkeQnD4PojGRDFQ-lSFKvCGBNa9TXb8F5US0G2gwItVyUcu-NWN0GNDSY65CGDphWffLYSwc5yQQO6wruYkNpp3YPgBfdqyd0brGs4inoEcrHqiB6m8JFQfp8g6vRn87vDp1EPS8qvetf18Djgr6QmsjYsNpxSU~6af5uKJZwa2ERWR0w__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology"},{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":132887,"name":"Transferrin","url":"https://www.academia.edu/Documents/in/Transferrin"},{"id":571342,"name":"Entamoeba histolytica","url":"https://www.academia.edu/Documents/in/Entamoeba_histolytica"},{"id":1476273,"name":"Giardia","url":"https://www.academia.edu/Documents/in/Giardia"},{"id":1938317,"name":"Innate Immune System","url":"https://www.academia.edu/Documents/in/Innate_Immune_System"},{"id":2558223,"name":"Cryptosporidium parvum","url":"https://www.academia.edu/Documents/in/Cryptosporidium_parvum"},{"id":2636453,"name":"Lactoferrin","url":"https://www.academia.edu/Documents/in/Lactoferrin"},{"id":3042534,"name":"battle","url":"https://www.academia.edu/Documents/in/battle"}],"urls":[{"id":38604497,"url":"https://doi.org/10.5772/66819"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") if (false) { Aedu.setUpFigureCarousel('profile-work-113549621-figures'); } }); </script> <div class="js-work-strip profile--work_container" data-work-id="113549620"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/113549620/Molecular_characterization_functional_expression_tissue_localization_and_protective_potential_of_a_Taenia_solium_fatty_acid_binding_protein"><img alt="Research paper thumbnail of Molecular characterization, functional expression, tissue localization and protective potential of a Taenia solium fatty acid-binding protein" class="work-thumbnail" src="https://attachments.academia-assets.com/110480513/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/113549620/Molecular_characterization_functional_expression_tissue_localization_and_protective_potential_of_a_Taenia_solium_fatty_acid_binding_protein">Molecular characterization, functional expression, tissue localization and protective potential of a Taenia solium fatty acid-binding protein</a></div><div class="wp-workCard_item"><span>Molecular and Biochemical Parasitology</span><span>, Dec 1, 2012</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The fatty acid-binding proteins (FABPs) comprise a family of proteins that are widely expressed i...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The fatty acid-binding proteins (FABPs) comprise a family of proteins that are widely expressed in animal cells and perform a variety of vital functions. Here, we report the identification, characterization, recombinant expression, tissue localization and protective potential of a Taenia solium FABP (TsFABP1). The TsFABP1 primary structure showed all the conserved residues characteristic of the subfamily iv of the intracellular Lipid-Binding Proteins (iLBPs), including those involved in the binding stabilization of the fatty acid molecule. Through a competitive binding assay we found that TsFABP1 is able to bind at least six different fatty acids with preference toward palmitic and stearic acid, suggesting that TsFABP1 is a member of the iLBP subfamily iv. Immunolocalization assays carried out on larval and adult tissues of four species of taeniids using anti-TsFABP1 hyperimmune sera produced in mice and rabbit, showed intense labeling in the tegument of the spiral canal and in subtegumental cytons of the larvae. These findings suggest that the spiral canal might be a major place for FA uptake in the developing scolex. In contrast, only subtegumental cytons in the adult worms stained positive. We propose that TsFABP1 is involved in the mechanism to mobilize fatty acids between compartments in the extensive syncytial tissue of taeniids. Protection assays carried out in a murine model of cysticercosis showed that subcutaneous immunization with TsFABP1 resulted in about 45% reduction of parasite load against an intraperitoneal challenge with Taenia crassiceps cysts. This reduction in parasite load correlated with the level of cellular and humoral immune responses against TsFABP1, as determined in spleen lymphocyte proliferation and ELISA testing.</span></div><div class="wp-workCard_item"><div class="carousel-container carousel-container--sm" id="profile-work-113549620-figures"><div class="prev-slide-container js-prev-button-container"><button aria-label="Previous" class="carousel-navigation-button js-profile-work-113549620-figures-prev"><span class="material-symbols-outlined" style="font-size: 24px" translate="no">arrow_back_ios</span></button></div><div class="slides-container js-slides-container"><figure class="figure-slide-container"><a href="https://www.academia.edu/figures/53628430/figure-2-recombinant-expression-and-immune-recognition-of"><img alt="Fig. 2. Recombinant expression and immune recognition of TsFABP1.Lanes (A-C): SDS-PAGE of (A) molecular weight markers, (B) crude extract of BL21PLys trans- formed cells and (C) purified TsFABP1 after nickel affinity chromatography; lanes D and E: Western blots of (D) crude antigenic extract (15 wg) of T. solium cysticerci reacted with a hyperimmune anti-TsFABP1 mice serum and (E) crude antigenic extract (15 wg) of T. crassiceps cysticerci reacted against a hyperimmune anti- TsFABP1 rabbit serum. Horseradish peroxidase-goat anti-mice IgG or anti-rabbit IgG were used as second antibodies. 4-Chloro-1-Naphtol was used as developer. " class="figure-slide-image" src="https://figures.academia-assets.com/110480513/figure_001.jpg" /></a></figure><figure class="figure-slide-container"><a href="https://www.academia.edu/figures/53628442/figure-4-humoral-immune-response-of-mice-after-immunization"><img alt="Fig. 4. Humoral immune response of mice after immunization with TsFABP1. Mice were immunized with 50 or 100 wg of TsFABP1, and bled at days 0, 14 and 28. At day 42, mice were challenged by intraperitoneal injection of 10 T. crassiceps cysts and sacrificed at day 94. Results from three independent experiments. " class="figure-slide-image" src="https://figures.academia-assets.com/110480513/figure_002.jpg" /></a></figure><figure class="figure-slide-container"><a href="https://www.academia.edu/figures/53628454/figure-3-tissue-immunolocalization-of-tsfabp-tissue-sections"><img alt="Fig. 3. Tissue immunolocalization of TsFABP1. Tissue sections from cysticerci of T. solium (A, D), T. pisiformis (B, E), T. crassiceps ORF (C, F) and WFU (G, J) strains, and adult worms of T. saginata (H, K) and T. solium (1, L). In A, B, C, G, H and I, the tissue sections were reacted with anti-TsFABP1 hyperimmune sera, whereas in D, E, F, J, K and L, were treated with preimmune sera. The corresponding second antibody was conjugated to horseradish peroxidase. Abbreviations: subtegumental cytons (sc), tegument (tg), bladder wall (bw), parenchyma (pa) and eggs (eg) are indicated by arrows. Reactions were developed with diamino benzidine. All sections were counterstained with hematoxylin and eosin. " class="figure-slide-image" src="https://figures.academia-assets.com/110480513/figure_003.jpg" /></a></figure><figure class="figure-slide-container"><a href="https://www.academia.edu/figures/53628462/figure-5-proliferation-assays-of-mice-spleen-lymphocytes"><img alt="Fig. 5. Proliferation assays of mice spleen lymphocytes after immunization with TsFABP1. Groups of mice were injected with 50 wg of TsFABP1 (immunized) or adju- vant alone (Adjuvant). One group of Adjuvant and one group of immunized mice were challenged with 10 cysts at day 42 and sacrificed at day 94. The other two groups were just sacrificed at day 42. Spleen lymphocytes were obtained after sac- rifice and stimulated with TsFABP1 or with a T. crassiceps crude antigenic extract. No significant differences were found when comparing groups 100 wg vs TSFABP1) (p>0.05). " class="figure-slide-image" src="https://figures.academia-assets.com/110480513/figure_004.jpg" /></a></figure><figure class="figure-slide-container"><a href="https://www.academia.edu/figures/53628470/table-1-tsfabp-binding-affinities-ka-values-in-nm-reported"><img alt="TsFABP1 binding affinities. 4 Ka values in nM, reported as an average of 3 experiments + SD. Table 1 " class="figure-slide-image" src="https://figures.academia-assets.com/110480513/table_001.jpg" /></a></figure><figure class="figure-slide-container"><a href="https://www.academia.edu/figures/53628482/figure-1-molecular-characterization-of-tsfabp-gene-structure"><img alt="Fig. 1. Molecular characterization of TsFABP1. (A) Gene structure of Tsfabp1. The 402 bp coding sequence in mRNA is translated into the 133 amino acid residues (~15 KDa) product; (B) Alignment of selected FABPs amino acid sequences. Abbreviations (GenBank accession numbers): T. solium TsFABP1 (JQ929049), TsFABP2 (JX470484), E. gra- nulosus EgFABP1 (AAK12096) and EgFABP2 (AAK12094), F. gigantica FgFABP (AAD23998), F. hepatica FASHE3 (Q9U1G6), S. mansoni Sm14 (AAT39384), S. japonicum SjFABPc (AAA64425), human FABP8 (NP_002668). In the sequence alignment, identities with TsFABP1 are shown as blank and gaps are shown as dashes. Residues involved in the stabilization of the ligand carboxyl and hydrophobic termini, are shown in dark and light gray, respectively. " class="figure-slide-image" src="https://figures.academia-assets.com/110480513/table_002.jpg" /></a></figure><figure class="figure-slide-container"><a href="https://www.academia.edu/figures/53628489/table-2-was-determined-for-the-immunized-mice-by-comparing"><img alt="* p was determined for the immunized mice by comparing with the adjuvant-only group.’ Results from 3 independent experiments. Protection studies in the murine model of T. crassiceps. Table 2 " class="figure-slide-image" src="https://figures.academia-assets.com/110480513/table_003.jpg" /></a></figure></div><div class="next-slide-container js-next-button-container"><button aria-label="Next" class="carousel-navigation-button js-profile-work-113549620-figures-next"><span class="material-symbols-outlined" style="font-size: 24px" translate="no">arrow_forward_ios</span></button></div></div></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="7d3a5a5913c9ac2503db030700f21584" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":110480513,"asset_id":113549620,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/110480513/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="113549620"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="113549620"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 113549620; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=113549620]").text(description); $(".js-view-count[data-work-id=113549620]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 113549620; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='113549620']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "7d3a5a5913c9ac2503db030700f21584" } } $('.js-work-strip[data-work-id=113549620]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":113549620,"title":"Molecular characterization, functional expression, tissue localization and protective potential of a Taenia solium fatty acid-binding protein","translated_title":"","metadata":{"publisher":"Elsevier BV","grobid_abstract":"The fatty acid-binding proteins (FABPs) comprise a family of proteins that are widely expressed in animal cells and perform a variety of vital functions. Here, we report the identification, characterization, recombinant expression, tissue localization and protective potential of a Taenia solium FABP (TsFABP1). The TsFABP1 primary structure showed all the conserved residues characteristic of the subfamily iv of the intracellular Lipid-Binding Proteins (iLBPs), including those involved in the binding stabilization of the fatty acid molecule. Through a competitive binding assay we found that TsFABP1 is able to bind at least six different fatty acids with preference toward palmitic and stearic acid, suggesting that TsFABP1 is a member of the iLBP subfamily iv. Immunolocalization assays carried out on larval and adult tissues of four species of taeniids using anti-TsFABP1 hyperimmune sera produced in mice and rabbit, showed intense labeling in the tegument of the spiral canal and in subtegumental cytons of the larvae. These findings suggest that the spiral canal might be a major place for FA uptake in the developing scolex. In contrast, only subtegumental cytons in the adult worms stained positive. We propose that TsFABP1 is involved in the mechanism to mobilize fatty acids between compartments in the extensive syncytial tissue of taeniids. Protection assays carried out in a murine model of cysticercosis showed that subcutaneous immunization with TsFABP1 resulted in about 45% reduction of parasite load against an intraperitoneal challenge with Taenia crassiceps cysts. This reduction in parasite load correlated with the level of cellular and humoral immune responses against TsFABP1, as determined in spleen lymphocyte proliferation and ELISA testing.","publication_date":{"day":1,"month":12,"year":2012,"errors":{}},"publication_name":"Molecular and Biochemical Parasitology","grobid_abstract_attachment_id":110480513},"translated_abstract":null,"internal_url":"https://www.academia.edu/113549620/Molecular_characterization_functional_expression_tissue_localization_and_protective_potential_of_a_Taenia_solium_fatty_acid_binding_protein","translated_internal_url":"","created_at":"2024-01-15T12:51:34.177-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":36697270,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":110480513,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/110480513/thumbnails/1.jpg","file_name":"j.molbiopara.2012.10.00220240115-1-3gb6fm.pdf","download_url":"https://www.academia.edu/attachments/110480513/download_file","bulk_download_file_name":"Molecular_characterization_functional_ex.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/110480513/j.molbiopara.2012.10.00220240115-1-3gb6fm-libre.pdf?1705355553=\u0026response-content-disposition=attachment%3B+filename%3DMolecular_characterization_functional_ex.pdf\u0026Expires=1743230062\u0026Signature=dScZ~48P6TirXJwkm4~ck7rBnAgSaj4ySTRWurRaR6PAX1dBd1tSX21zi3VMpRXN76ETvY8N1M25-4hdUvJSqS1ZsUXM3~4uJ6pRlnnh9DBN~Z7meISaqZepmQ60J48IGhwJnnm5IfJGpaIuuqyvLLb3hW~oRRzKTdBDx3QqpBGqCvceUZutn692S4QM5gSDrv1c~W2D6LSj92NMJPclUheeH0E2olUXN6uGBV94Om537w8n6F~0-4E7qgwJbSpdq3FA9garPv-Qsurb2AoGU~pYzNh8ey-FX8xiTjaLufHu3NgWd7Gx-d~kM2ldvrUIs3Ol965PUWIcPol6FCYL-A__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Molecular_characterization_functional_expression_tissue_localization_and_protective_potential_of_a_Taenia_solium_fatty_acid_binding_protein","translated_slug":"","page_count":9,"language":"en","content_type":"Work","summary":"The fatty acid-binding proteins (FABPs) comprise a family of proteins that are widely expressed in animal cells and perform a variety of vital functions. Here, we report the identification, characterization, recombinant expression, tissue localization and protective potential of a Taenia solium FABP (TsFABP1). The TsFABP1 primary structure showed all the conserved residues characteristic of the subfamily iv of the intracellular Lipid-Binding Proteins (iLBPs), including those involved in the binding stabilization of the fatty acid molecule. Through a competitive binding assay we found that TsFABP1 is able to bind at least six different fatty acids with preference toward palmitic and stearic acid, suggesting that TsFABP1 is a member of the iLBP subfamily iv. Immunolocalization assays carried out on larval and adult tissues of four species of taeniids using anti-TsFABP1 hyperimmune sera produced in mice and rabbit, showed intense labeling in the tegument of the spiral canal and in subtegumental cytons of the larvae. These findings suggest that the spiral canal might be a major place for FA uptake in the developing scolex. In contrast, only subtegumental cytons in the adult worms stained positive. We propose that TsFABP1 is involved in the mechanism to mobilize fatty acids between compartments in the extensive syncytial tissue of taeniids. Protection assays carried out in a murine model of cysticercosis showed that subcutaneous immunization with TsFABP1 resulted in about 45% reduction of parasite load against an intraperitoneal challenge with Taenia crassiceps cysts. This reduction in parasite load correlated with the level of cellular and humoral immune responses against TsFABP1, as determined in spleen lymphocyte proliferation and ELISA testing.","owner":{"id":36697270,"first_name":"Julio","middle_initials":null,"last_name":"Carrero","page_name":"JulioCarrero1","domain_name":"independent","created_at":"2015-10-21T12:09:01.815-07:00","display_name":"Julio Carrero","url":"https://independent.academia.edu/JulioCarrero1"},"attachments":[{"id":110480513,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/110480513/thumbnails/1.jpg","file_name":"j.molbiopara.2012.10.00220240115-1-3gb6fm.pdf","download_url":"https://www.academia.edu/attachments/110480513/download_file","bulk_download_file_name":"Molecular_characterization_functional_ex.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/110480513/j.molbiopara.2012.10.00220240115-1-3gb6fm-libre.pdf?1705355553=\u0026response-content-disposition=attachment%3B+filename%3DMolecular_characterization_functional_ex.pdf\u0026Expires=1743230062\u0026Signature=dScZ~48P6TirXJwkm4~ck7rBnAgSaj4ySTRWurRaR6PAX1dBd1tSX21zi3VMpRXN76ETvY8N1M25-4hdUvJSqS1ZsUXM3~4uJ6pRlnnh9DBN~Z7meISaqZepmQ60J48IGhwJnnm5IfJGpaIuuqyvLLb3hW~oRRzKTdBDx3QqpBGqCvceUZutn692S4QM5gSDrv1c~W2D6LSj92NMJPclUheeH0E2olUXN6uGBV94Om537w8n6F~0-4E7qgwJbSpdq3FA9garPv-Qsurb2AoGU~pYzNh8ey-FX8xiTjaLufHu3NgWd7Gx-d~kM2ldvrUIs3Ol965PUWIcPol6FCYL-A__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":145,"name":"Biochemistry","url":"https://www.academia.edu/Documents/in/Biochemistry"},{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences"},{"id":67484,"name":"Sequence alignment","url":"https://www.academia.edu/Documents/in/Sequence_alignment"},{"id":72314,"name":"Fatty acids","url":"https://www.academia.edu/Documents/in/Fatty_acids"},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice"},{"id":129391,"name":"Gene Order","url":"https://www.academia.edu/Documents/in/Gene_Order"},{"id":136892,"name":"Immunization","url":"https://www.academia.edu/Documents/in/Immunization"},{"id":809881,"name":"Amino Acid Sequence","url":"https://www.academia.edu/Documents/in/Amino_Acid_Sequence"},{"id":990417,"name":"Recombinant Proteins","url":"https://www.academia.edu/Documents/in/Recombinant_Proteins"},{"id":1010725,"name":"Protein Binding","url":"https://www.academia.edu/Documents/in/Protein_Binding"},{"id":1458519,"name":"Cysticercosis","url":"https://www.academia.edu/Documents/in/Cysticercosis"},{"id":1509323,"name":"Fatty Acid Binding Protein","url":"https://www.academia.edu/Documents/in/Fatty_Acid_Binding_Protein"},{"id":2060319,"name":"Taenia solium","url":"https://www.academia.edu/Documents/in/Taenia_solium"},{"id":2467566,"name":"Molecular Sequence Data","url":"https://www.academia.edu/Documents/in/Molecular_Sequence_Data"},{"id":3763225,"name":"Medical and Health Sciences","url":"https://www.academia.edu/Documents/in/Medical_and_Health_Sciences"}],"urls":[{"id":38604496,"url":"https://doi.org/10.1016/j.molbiopara.2012.10.002"}]}, dispatcherData: dispatcherData }); 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Focused on defining the core promoter characteristics, the study identifies significant deletion and mutation effects on transcriptional activity. The findings contribute to a deeper understanding of transcriptional regulation in early-branching eukaryotes and highlight parallels with higher eukaryotic systems.","publication_date":{"day":1,"month":11,"year":1999,"errors":{}},"publication_name":"Molecular and Biochemical Parasitology"},"translated_abstract":null,"internal_url":"https://www.academia.edu/113549619/Upstream_regulatory_sequences_required_for_expression_of_the_Trichomonas_vaginalis_%CE%B1_succinyl_CoA_synthetase_gene","translated_internal_url":"","created_at":"2024-01-15T12:51:34.043-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":36697270,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":110480519,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/110480519/thumbnails/1.jpg","file_name":"s0166-685128992900137-120240115-1-s4o4yh.pdf","download_url":"https://www.academia.edu/attachments/110480519/download_file","bulk_download_file_name":"Upstream_regulatory_sequences_required_f.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/110480519/s0166-685128992900137-120240115-1-s4o4yh-libre.pdf?1705355546=\u0026response-content-disposition=attachment%3B+filename%3DUpstream_regulatory_sequences_required_f.pdf\u0026Expires=1743230062\u0026Signature=Cb5it~~eFinKTKCYwesBGSwWtuZgqqUwe5~dkkL~Q3ul0640P6rNXjq2DcVTLp2dGSoWpCCLZsu1wt3N046IswnqQ8tdPuv90rrdBoTnZ3jvUR4kc8G5t2oPQm2PNFR-f381Tg2oGnbY8Dk6B7g8xawBr8uY9qIjqsMW6Yq~8uz-irLNdKszgaYZbFyAGc~tic7JtZH5ZrG2ulMijTxhaFkhG2AR3Vvmz~cxS4-3Z5lI69AsLPYLzBaBR0HzypCLCnXxMZdh4docvdH3qRtp-ZvQvIofBK56RcfUHVJb6LObHiPEUWtT7oVOx7ZtjEQiLE9ojG3L9ZdOQ7qyftdNBA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Upstream_regulatory_sequences_required_for_expression_of_the_Trichomonas_vaginalis_α_succinyl_CoA_synthetase_gene","translated_slug":"","page_count":7,"language":"en","content_type":"Work","summary":null,"owner":{"id":36697270,"first_name":"Julio","middle_initials":null,"last_name":"Carrero","page_name":"JulioCarrero1","domain_name":"independent","created_at":"2015-10-21T12:09:01.815-07:00","display_name":"Julio Carrero","url":"https://independent.academia.edu/JulioCarrero1"},"attachments":[{"id":110480519,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/110480519/thumbnails/1.jpg","file_name":"s0166-685128992900137-120240115-1-s4o4yh.pdf","download_url":"https://www.academia.edu/attachments/110480519/download_file","bulk_download_file_name":"Upstream_regulatory_sequences_required_f.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/110480519/s0166-685128992900137-120240115-1-s4o4yh-libre.pdf?1705355546=\u0026response-content-disposition=attachment%3B+filename%3DUpstream_regulatory_sequences_required_f.pdf\u0026Expires=1743230062\u0026Signature=Cb5it~~eFinKTKCYwesBGSwWtuZgqqUwe5~dkkL~Q3ul0640P6rNXjq2DcVTLp2dGSoWpCCLZsu1wt3N046IswnqQ8tdPuv90rrdBoTnZ3jvUR4kc8G5t2oPQm2PNFR-f381Tg2oGnbY8Dk6B7g8xawBr8uY9qIjqsMW6Yq~8uz-irLNdKszgaYZbFyAGc~tic7JtZH5ZrG2ulMijTxhaFkhG2AR3Vvmz~cxS4-3Z5lI69AsLPYLzBaBR0HzypCLCnXxMZdh4docvdH3qRtp-ZvQvIofBK56RcfUHVJb6LObHiPEUWtT7oVOx7ZtjEQiLE9ojG3L9ZdOQ7qyftdNBA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":156,"name":"Genetics","url":"https://www.academia.edu/Documents/in/Genetics"},{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences"},{"id":74780,"name":"Mutation","url":"https://www.academia.edu/Documents/in/Mutation"},{"id":131572,"name":"Trichomonas vaginalis","url":"https://www.academia.edu/Documents/in/Trichomonas_vaginalis"},{"id":181936,"name":"Gene","url":"https://www.academia.edu/Documents/in/Gene"},{"id":809882,"name":"Base Sequence","url":"https://www.academia.edu/Documents/in/Base_Sequence"},{"id":1763968,"name":"Gene Expression Regulation","url":"https://www.academia.edu/Documents/in/Gene_Expression_Regulation"},{"id":2467566,"name":"Molecular Sequence Data","url":"https://www.academia.edu/Documents/in/Molecular_Sequence_Data"},{"id":3763225,"name":"Medical and Health Sciences","url":"https://www.academia.edu/Documents/in/Medical_and_Health_Sciences"}],"urls":[{"id":38604495,"url":"https://doi.org/10.1016/s0166-6851(99)00137-1"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") if (false) { Aedu.setUpFigureCarousel('profile-work-113549619-figures'); } }); </script> <div class="js-work-strip profile--work_container" data-work-id="113549618"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/113549618/Intestinal_amoebiasis_160_years_of_its_first_detection_and_still_remains_as_a_health_problem_in_developing_countries"><img alt="Research paper thumbnail of Intestinal amoebiasis: 160 years of its first detection and still remains as a health problem in developing countries" class="work-thumbnail" src="https://attachments.academia-assets.com/110480515/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/113549618/Intestinal_amoebiasis_160_years_of_its_first_detection_and_still_remains_as_a_health_problem_in_developing_countries">Intestinal amoebiasis: 160 years of its first detection and still remains as a health problem in developing countries</a></div><div class="wp-workCard_item"><span>International Journal of Medical Microbiology</span><span>, 2020</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Amoebiasis is a parasitic disease caused by Entamoeba histolytica (E. histolytica), an extracellu...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Amoebiasis is a parasitic disease caused by Entamoeba histolytica (E. histolytica), an extracellular enteric protozoan. This infection mainly affects people from developing countries with limited hygiene conditions, where it is endemic. Infective cysts are transmitted by the fecal-oral route, excysting in the terminal ileum and producing invasive trophozoites (amoebae). E. histolytica mainly lives in the large intestine without causing symptoms; however, possibly as a result of so far unknown signals, the amoebae invade the mucosa and epithelium causing intestinal amoebiasis. E. histolytica possesses different mechanisms of pathogenicity for the adherence to the intestinal epithelium and for degrading extracellular matrix proteins, producing tissue lesions that progress to abscesses and a host acute inflammatory response. Much information has been obtained regarding the virulence factors, metabolism, mechanisms of pathogenicity, and the host immune response against this parasite; in addition, alternative treatments to metronidazole are continually emerging. An accesible and low-cost diagnostic method that can distinguish E. histolytica from the most nonpathogenic amoebae and an effective vaccine are necessary for protecting against amoebiasis. However, research about the disease and its prevention has been a challenge due to the relationship between E. histolytica and the host during the distinct stages of the disease is multifaceted. In this review, we analyze the interaction between the parasite, the human host, and the colon microbiota or pathogenic microorganisms, which together give rise to intestinal amoebiasis.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="baf03ac408715d395ca2404d0cd0d61a" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":110480515,"asset_id":113549618,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/110480515/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="113549618"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="113549618"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 113549618; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=113549618]").text(description); $(".js-view-count[data-work-id=113549618]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 113549618; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='113549618']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "baf03ac408715d395ca2404d0cd0d61a" } } $('.js-work-strip[data-work-id=113549618]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":113549618,"title":"Intestinal amoebiasis: 160 years of its first detection and still remains as a health problem in developing countries","translated_title":"","metadata":{"publisher":"Elsevier BV","ai_title_tag":"Amoebiasis: 160 Years of Challenges in Developing Nations","grobid_abstract":"Amoebiasis is a parasitic disease caused by Entamoeba histolytica (E. histolytica), an extracellular enteric protozoan. 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An accesible and low-cost diagnostic method that can distinguish E. histolytica from the most nonpathogenic amoebae and an effective vaccine are necessary for protecting against amoebiasis. However, research about the disease and its prevention has been a challenge due to the relationship between E. histolytica and the host during the distinct stages of the disease is multifaceted. 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An accesible and low-cost diagnostic method that can distinguish E. histolytica from the most nonpathogenic amoebae and an effective vaccine are necessary for protecting against amoebiasis. However, research about the disease and its prevention has been a challenge due to the relationship between E. histolytica and the host during the distinct stages of the disease is multifaceted. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") if (false) { Aedu.setUpFigureCarousel('profile-work-113549617-figures'); } }); </script> <div class="js-work-strip profile--work_container" data-work-id="113549616"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/113549616/Cloning_sequencing_and_functional_expression_of_cytosolic_malate_dehydrogenase_from_Taenia_solium_Purification_and_characterization_of_the_recombinant_enzyme"><img alt="Research paper thumbnail of Cloning, sequencing and functional expression of cytosolic malate dehydrogenase from Taenia solium: Purification and characterization of the recombinant enzyme" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title">Cloning, sequencing and functional expression of cytosolic malate dehydrogenase from Taenia solium: Purification and characterization of the recombinant enzyme</div><div class="wp-workCard_item"><span>Experimental Parasitology</span><span>, Jul 1, 2011</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">We report herein the complete coding sequence of a Taenia solium cytosolic malate dehydrogenase (...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">We report herein the complete coding sequence of a Taenia solium cytosolic malate dehydrogenase (TscMDH). The cDNA fragment, identified from the T. solium genome project database, encodes a protein of 332 amino acid residues with an estimated molecular weight of 36517Da. For recombinant expression, the full length coding sequence was cloned into pET23a. After successful expression and enzyme purification, isoelectrofocusing gel electrophoresis allowed to confirm the calculated pI value at 8.1, as deduced from the amino acid sequence. The recombinant protein (r-TscMDH) showed MDH activity of 409U/mg in the reduction of oxaloacetate, with neither lactate dehydrogenase activity nor NADPH selectivity. Optimum pH for enzyme activity was 7.6 for oxaloacetate reduction and 9.6 for malate oxidation. K(cat) values for oxaloacetate, malate, NAD, and NADH were 665, 47, 385, and 962s(-1), respectively. Additionally, a partial characterization of TsMDH gene structure after analysis of a 1.56Kb genomic contig assembly is also reported.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="113549616"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="113549616"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 113549616; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=113549616]").text(description); $(".js-view-count[data-work-id=113549616]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 113549616; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='113549616']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=113549616]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":113549616,"title":"Cloning, sequencing and functional expression of cytosolic malate dehydrogenase from Taenia solium: Purification and characterization of the recombinant enzyme","translated_title":"","metadata":{"abstract":"We report herein the complete coding sequence of a Taenia solium cytosolic malate dehydrogenase (TscMDH). 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") if (false) { Aedu.setUpFigureCarousel('profile-work-113549616-figures'); } }); </script> <div class="js-work-strip profile--work_container" data-work-id="113549615"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/113549615/Host_Immune_Responses_Against_Intestinal_Unicellular_Parasites_and_Their_Role_in_Pathogenesis_and_Protection"><img alt="Research paper thumbnail of Host Immune Responses Against Intestinal Unicellular Parasites and Their Role in Pathogenesis and Protection" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title">Host Immune Responses Against Intestinal Unicellular Parasites and Their Role in Pathogenesis and Protection</div><div class="wp-workCard_item"><span>Elsevier eBooks</span><span>, 2022</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="113549615"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="113549615"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 113549615; 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") if (false) { Aedu.setUpFigureCarousel('profile-work-113549615-figures'); } }); </script> <div class="js-work-strip profile--work_container" data-work-id="113549614"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/113549614/Effects_of_solvent_free_amine_functionalization_of_graphene_oxide_and_nanodiamond_on_bacterial_growth"><img alt="Research paper thumbnail of Effects of solvent-free amine functionalization of graphene oxide and nanodiamond on bacterial growth" class="work-thumbnail" src="https://attachments.academia-assets.com/110480511/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/113549614/Effects_of_solvent_free_amine_functionalization_of_graphene_oxide_and_nanodiamond_on_bacterial_growth">Effects of solvent-free amine functionalization of graphene oxide and nanodiamond on bacterial growth</a></div><div class="wp-workCard_item"><span>Fullerenes Nanotubes and Carbon Nanostructures</span><span>, Aug 24, 2020</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">We studied the effect of covalent functionalization of graphene oxide (GO) and nanodiamond (ND) w...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">We studied the effect of covalent functionalization of graphene oxide (GO) and nanodiamond (ND) with octadecylamine (ODA) on bacterial growth (a series of experiments was performed also with pristine single-walled carbon nanotubes [SWNTs] for comparison). The bacteria tested were Escherichia coli and Staphylococcus aureus, which represent Gram-positive and Gram-negative types, respectively, and are of importance for the environment and human health. We found that pristine GO is the most toxic nanomaterial in both bacteria species, which exhibits a dose-dependent behavior. SWNTs show toxicity only against S. aureus at the higher concentrations of 1.0 and 10 mg/mL. Pristine ND, as expected, was found to be the least toxic against both species of bacteria, and in the experiments with S. aureus it even showed a viability amplifier activity at 10 mg/ mL concentration. The use of ODA-functionalized nanomaterials generally changed the toxicity behavior, neutralizing the antibacterial effect of GO (for both S. aureus and E. coli), but making ODA-functionalized ND more toxic as compared to pristine material (with respect to S. aureus).</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="d9d734aa368e1a08f97877827a89b04b" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":110480511,"asset_id":113549614,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/110480511/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="113549614"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="113549614"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 113549614; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=113549614]").text(description); $(".js-view-count[data-work-id=113549614]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 113549614; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='113549614']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "d9d734aa368e1a08f97877827a89b04b" } } $('.js-work-strip[data-work-id=113549614]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":113549614,"title":"Effects of solvent-free amine functionalization of graphene oxide and nanodiamond on bacterial growth","translated_title":"","metadata":{"publisher":"Taylor \u0026 Francis","ai_title_tag":"Amine Functionalization of Graphene Oxide Effects","grobid_abstract":"We studied the effect of covalent functionalization of graphene oxide (GO) and nanodiamond (ND) with octadecylamine (ODA) on bacterial growth (a series of experiments was performed also with pristine single-walled carbon nanotubes [SWNTs] for comparison). The bacteria tested were Escherichia coli and Staphylococcus aureus, which represent Gram-positive and Gram-negative types, respectively, and are of importance for the environment and human health. We found that pristine GO is the most toxic nanomaterial in both bacteria species, which exhibits a dose-dependent behavior. SWNTs show toxicity only against S. aureus at the higher concentrations of 1.0 and 10 mg/mL. Pristine ND, as expected, was found to be the least toxic against both species of bacteria, and in the experiments with S. aureus it even showed a viability amplifier activity at 10 mg/ mL concentration. 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The bacteria tested were Escherichia coli and Staphylococcus aureus, which represent Gram-positive and Gram-negative types, respectively, and are of importance for the environment and human health. We found that pristine GO is the most toxic nanomaterial in both bacteria species, which exhibits a dose-dependent behavior. SWNTs show toxicity only against S. aureus at the higher concentrations of 1.0 and 10 mg/mL. Pristine ND, as expected, was found to be the least toxic against both species of bacteria, and in the experiments with S. aureus it even showed a viability amplifier activity at 10 mg/ mL concentration. 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South American count...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Background: Raw vegetables have been considered vehicles of enteroparasites. South American countries are among the most important exporters of fresh vegetables; Ecuador has tropical climates and soils rich in organic matter that allow it to harvest throughout the year for sale to different countries. The aim of the study was to assess the occurrence of the parasitic contamination of fruits, vegetables and leafy greens grown in an agricultural area of the Ecuadorian Andes. Methods: A field study, cross-sectional, snowball sampling was conducted on 1,416 samples (516 fruits, 488 vegetables, and 412 leafy greens). Each sample were washed with water, and the resulting solution after removing the vegetables, was subjected to 24-hour sedimentation. The concentrated sediment underwent microscopic analysis. Results: Parasites were detected in 63.4% of the samples, leafy greens were the most contaminated (76.9%) (P&lt;0.0001), (vegetables 67.8% and fruit 48.4%), of these, cabbage (100%), on...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="8da4e6669d8ed056cda05c39d3288082" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":110480458,"asset_id":113549613,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/110480458/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="113549613"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="113549613"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 113549613; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=113549613]").text(description); $(".js-view-count[data-work-id=113549613]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 113549613; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='113549613']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "8da4e6669d8ed056cda05c39d3288082" } } $('.js-work-strip[data-work-id=113549613]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":113549613,"title":"Field study of parasitic contamination of fruits, vegetables and leafy greens in the Ecuadorian Andes","translated_title":"","metadata":{"abstract":"Background: Raw vegetables have been considered vehicles of enteroparasites. South American countries are among the most important exporters of fresh vegetables; Ecuador has tropical climates and soils rich in organic matter that allow it to harvest throughout the year for sale to different countries. The aim of the study was to assess the occurrence of the parasitic contamination of fruits, vegetables and leafy greens grown in an agricultural area of the Ecuadorian Andes. Methods: A field study, cross-sectional, snowball sampling was conducted on 1,416 samples (516 fruits, 488 vegetables, and 412 leafy greens). Each sample were washed with water, and the resulting solution after removing the vegetables, was subjected to 24-hour sedimentation. The concentrated sediment underwent microscopic analysis. Results: Parasites were detected in 63.4% of the samples, leafy greens were the most contaminated (76.9%) (P\u0026lt;0.0001), (vegetables 67.8% and fruit 48.4%), of these, cabbage (100%), on...","publisher":"F1000 Research Ltd","publication_name":"F1000Research"},"translated_abstract":"Background: Raw vegetables have been considered vehicles of enteroparasites. South American countries are among the most important exporters of fresh vegetables; Ecuador has tropical climates and soils rich in organic matter that allow it to harvest throughout the year for sale to different countries. The aim of the study was to assess the occurrence of the parasitic contamination of fruits, vegetables and leafy greens grown in an agricultural area of the Ecuadorian Andes. Methods: A field study, cross-sectional, snowball sampling was conducted on 1,416 samples (516 fruits, 488 vegetables, and 412 leafy greens). Each sample were washed with water, and the resulting solution after removing the vegetables, was subjected to 24-hour sedimentation. The concentrated sediment underwent microscopic analysis. Results: Parasites were detected in 63.4% of the samples, leafy greens were the most contaminated (76.9%) (P\u0026lt;0.0001), (vegetables 67.8% and fruit 48.4%), of these, cabbage (100%), on...","internal_url":"https://www.academia.edu/113549613/Field_study_of_parasitic_contamination_of_fruits_vegetables_and_leafy_greens_in_the_Ecuadorian_Andes","translated_internal_url":"","created_at":"2024-01-15T12:51:33.222-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":36697270,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":110480458,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/110480458/thumbnails/1.jpg","file_name":"pdf.pdf","download_url":"https://www.academia.edu/attachments/110480458/download_file","bulk_download_file_name":"Field_study_of_parasitic_contamination_o.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/110480458/pdf-libre.pdf?1705355557=\u0026response-content-disposition=attachment%3B+filename%3DField_study_of_parasitic_contamination_o.pdf\u0026Expires=1743230063\u0026Signature=F7feIGt5yB7W8AvzhzhBr0qgNw5pRrxbjhdhc4EubTrTtwlPZPBnu0Vg~se5IFo-eUZU3ymNFu9ZS4fMvp~iHSimVberjr4zPhIRj8Sc-YvC-8k~lQkL-lORkC1GY1DjyuG4-Lg6efkYMldkWKdqZrER0yqtiRNJ4BYavYVps71N0uId-3nUba8-CCqCIROEDDqckVMwd06DTFNY-xzDeQNYl9gHh5WLk6DQp4c1gciKjX3IHPZgKtjVuAaDGmbFs2GtJWxBza7PLZWehZQxXrbWXh2qkWPS0o~DNtRM6O9RcHYUXMS8ejqJ5itLxv8p3c11l-CykK9wv6sfMKp34g__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Field_study_of_parasitic_contamination_of_fruits_vegetables_and_leafy_greens_in_the_Ecuadorian_Andes","translated_slug":"","page_count":16,"language":"en","content_type":"Work","summary":"Background: Raw vegetables have been considered vehicles of enteroparasites. South American countries are among the most important exporters of fresh vegetables; Ecuador has tropical climates and soils rich in organic matter that allow it to harvest throughout the year for sale to different countries. The aim of the study was to assess the occurrence of the parasitic contamination of fruits, vegetables and leafy greens grown in an agricultural area of the Ecuadorian Andes. Methods: A field study, cross-sectional, snowball sampling was conducted on 1,416 samples (516 fruits, 488 vegetables, and 412 leafy greens). Each sample were washed with water, and the resulting solution after removing the vegetables, was subjected to 24-hour sedimentation. The concentrated sediment underwent microscopic analysis. Results: Parasites were detected in 63.4% of the samples, leafy greens were the most contaminated (76.9%) (P\u0026lt;0.0001), (vegetables 67.8% and fruit 48.4%), of these, cabbage (100%), on...","owner":{"id":36697270,"first_name":"Julio","middle_initials":null,"last_name":"Carrero","page_name":"JulioCarrero1","domain_name":"independent","created_at":"2015-10-21T12:09:01.815-07:00","display_name":"Julio Carrero","url":"https://independent.academia.edu/JulioCarrero1"},"attachments":[{"id":110480458,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/110480458/thumbnails/1.jpg","file_name":"pdf.pdf","download_url":"https://www.academia.edu/attachments/110480458/download_file","bulk_download_file_name":"Field_study_of_parasitic_contamination_o.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/110480458/pdf-libre.pdf?1705355557=\u0026response-content-disposition=attachment%3B+filename%3DField_study_of_parasitic_contamination_o.pdf\u0026Expires=1743230063\u0026Signature=F7feIGt5yB7W8AvzhzhBr0qgNw5pRrxbjhdhc4EubTrTtwlPZPBnu0Vg~se5IFo-eUZU3ymNFu9ZS4fMvp~iHSimVberjr4zPhIRj8Sc-YvC-8k~lQkL-lORkC1GY1DjyuG4-Lg6efkYMldkWKdqZrER0yqtiRNJ4BYavYVps71N0uId-3nUba8-CCqCIROEDDqckVMwd06DTFNY-xzDeQNYl9gHh5WLk6DQp4c1gciKjX3IHPZgKtjVuAaDGmbFs2GtJWxBza7PLZWehZQxXrbWXh2qkWPS0o~DNtRM6O9RcHYUXMS8ejqJ5itLxv8p3c11l-CykK9wv6sfMKp34g__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"},{"id":110480459,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/110480459/thumbnails/1.jpg","file_name":"pdf.pdf","download_url":"https://www.academia.edu/attachments/110480459/download_file","bulk_download_file_name":"Field_study_of_parasitic_contamination_o.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/110480459/pdf-libre.pdf?1705355554=\u0026response-content-disposition=attachment%3B+filename%3DField_study_of_parasitic_contamination_o.pdf\u0026Expires=1743230063\u0026Signature=Vv8pPVDpcgOxt-lK-UwzuRRts84EUKBAPFysqh5QCZeuuwP3Rrp49LgdF8pv27FV-xJJJOaPJwfI5YvcTpPKp1-eFgXpS5I~JUSiZbEniboUnms19AfLSoPufzU0VrL8p8Av44saE8robQ~YMffystUR3dV2f4U8u5qmq4hy64sGiQHORHUYH-iGtT7O7YF2IonCaWxJmf3JT0JmjBTGnBnkIPfYafE73kQnPBvZ8yqOC4bW~8OicFy1kIJFglSxlwzkHE0VPWwRS1YYqu4ySnj7Y0zwz-kMiY4G2u-y5dwnQq8Q1SKkiSEJ~iRp5SxK1aUmrIaxBO-utBXFoA~0Hw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":24173,"name":"F Mri Research","url":"https://www.academia.edu/Documents/in/F_Mri_Research"}],"urls":[{"id":38604489,"url":"https://f1000research.com/articles/12-532/v1/pdf"}]}, dispatcherData: dispatcherData }); 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The peptide P5 was demonstrated to be safe and immunogenic, inducing specific IgG antibodies in animal models. Although neutralizing antibody titers were low, findings support P5's potential in designing a vaccine, with plans to enhance immunogenicity by incorporating more epitopes.","publication_name":"Vaccine"},"translated_abstract":null,"internal_url":"https://www.academia.edu/113549612/Towards_the_development_of_an_epitope_focused_vaccine_for_SARS_CoV_2","translated_internal_url":"","created_at":"2024-01-15T12:51:33.036-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":36697270,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":110480518,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/110480518/thumbnails/1.jpg","file_name":"pdf.pdf","download_url":"https://www.academia.edu/attachments/110480518/download_file","bulk_download_file_name":"Towards_the_development_of_an_epitope_fo.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/110480518/pdf-libre.pdf?1705355548=\u0026response-content-disposition=attachment%3B+filename%3DTowards_the_development_of_an_epitope_fo.pdf\u0026Expires=1743230063\u0026Signature=G89ZN5ovaEEUbj8kFHtI~a9gYqsAmSchVvx4gzuSGNdTOOd23PqYdPoSWxql5s8Q0oqmuSsW6Z~eXpC85c--ZQqjVr8CRibEH-aWWsGjHI3dRjJmzEna9Ctc1otIfjXtSyuuEZumZaG9F6iW048SOhhC-h-8Q6sBFOSPeK2bWCbGk35TyhOg4ryQwzAJKbsle2BnYWrY5Ogfi4aOvOW10WI2YO-ul4-FwlXF0Bru6bn4ajqeq8Q9apGiEH0n28E-U0r0h3CX9ADiA~zwcTddjPshfgXaTJP--27lY24K9a7sU03wqdUgDgiuyDeTfnGckSxQ1Dtpz35HyHdOReuGJw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Towards_the_development_of_an_epitope_focused_vaccine_for_SARS_CoV_2","translated_slug":"","page_count":11,"language":"en","content_type":"Work","summary":null,"owner":{"id":36697270,"first_name":"Julio","middle_initials":null,"last_name":"Carrero","page_name":"JulioCarrero1","domain_name":"independent","created_at":"2015-10-21T12:09:01.815-07:00","display_name":"Julio Carrero","url":"https://independent.academia.edu/JulioCarrero1"},"attachments":[{"id":110480518,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/110480518/thumbnails/1.jpg","file_name":"pdf.pdf","download_url":"https://www.academia.edu/attachments/110480518/download_file","bulk_download_file_name":"Towards_the_development_of_an_epitope_fo.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/110480518/pdf-libre.pdf?1705355548=\u0026response-content-disposition=attachment%3B+filename%3DTowards_the_development_of_an_epitope_fo.pdf\u0026Expires=1743230063\u0026Signature=G89ZN5ovaEEUbj8kFHtI~a9gYqsAmSchVvx4gzuSGNdTOOd23PqYdPoSWxql5s8Q0oqmuSsW6Z~eXpC85c--ZQqjVr8CRibEH-aWWsGjHI3dRjJmzEna9Ctc1otIfjXtSyuuEZumZaG9F6iW048SOhhC-h-8Q6sBFOSPeK2bWCbGk35TyhOg4ryQwzAJKbsle2BnYWrY5Ogfi4aOvOW10WI2YO-ul4-FwlXF0Bru6bn4ajqeq8Q9apGiEH0n28E-U0r0h3CX9ADiA~zwcTddjPshfgXaTJP--27lY24K9a7sU03wqdUgDgiuyDeTfnGckSxQ1Dtpz35HyHdOReuGJw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":8089,"name":"Virology","url":"https://www.academia.edu/Documents/in/Virology"},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences"},{"id":150847,"name":"Vaccine","url":"https://www.academia.edu/Documents/in/Vaccine"},{"id":357811,"name":"Antibody","url":"https://www.academia.edu/Documents/in/Antibody"},{"id":1314192,"name":"Immunogenicity","url":"https://www.academia.edu/Documents/in/Immunogenicity"},{"id":1426713,"name":"Antigen","url":"https://www.academia.edu/Documents/in/Antigen"},{"id":3398462,"name":"epitope","url":"https://www.academia.edu/Documents/in/epitope"},{"id":3763225,"name":"Medical and Health Sciences","url":"https://www.academia.edu/Documents/in/Medical_and_Health_Sciences"}],"urls":[{"id":38604488,"url":"https://api.elsevier.com/content/article/PII:S0264410X22011689?httpAccept=text/xml"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") if (false) { Aedu.setUpFigureCarousel('profile-work-113549612-figures'); } }); </script> <div class="js-work-strip profile--work_container" data-work-id="113549611"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/113549611/Adsorption_of_Phosphate_and_Nitrate_Ions_on_Oxidic_Substrates_Prepared_with_a_Variable_Charge_Lithological_Material"><img alt="Research paper thumbnail of Adsorption of Phosphate and Nitrate Ions on Oxidic Substrates Prepared with a Variable-Charge Lithological Material" class="work-thumbnail" src="https://attachments.academia-assets.com/110480457/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/113549611/Adsorption_of_Phosphate_and_Nitrate_Ions_on_Oxidic_Substrates_Prepared_with_a_Variable_Charge_Lithological_Material">Adsorption of Phosphate and Nitrate Ions on Oxidic Substrates Prepared with a Variable-Charge Lithological Material</a></div><div class="wp-workCard_item"><span>Water</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">This work evaluates phosphate and nitrate ion adsorption from aqueous solutions on calcined adsor...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">This work evaluates phosphate and nitrate ion adsorption from aqueous solutions on calcined adsorbent substrates of variable charge, prepared from three granulometric fractions of an oxidic lithological material. The adsorbent material was chemically characterized, and N2 gas adsorption (BET), X-ray diffraction, and DTA techniques were applied. The experimental conditions included the protonation of the beds with HCl and H2SO4 and the study of adsorption isotherms and kinetics. The lithological material was moderately acidic (pH 5) with very little solubility (electrical conductivity 0.013 dS m−1) and a low cation exchange capacity (53.67 cmol (+) kg−1). The protonation reaction was more efficient with HCl averaging 0.745 mmol versus 0.306 mmol with H2SO4. Likewise, the HCl-treated bed showed a better adsorption of PO4−3 ions (3.296 mg/100 g bed) compared to the H2SO4-treated bed (2.579 mg/100 g bed). The isotherms showed great affinity of the PO4−3 ions with the oxide surface, and ...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="67f311868a9c6d4211ca4d4d3dcca3f0" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":110480457,"asset_id":113549611,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/110480457/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="113549611"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="113549611"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 113549611; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=113549611]").text(description); $(".js-view-count[data-work-id=113549611]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 113549611; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='113549611']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "67f311868a9c6d4211ca4d4d3dcca3f0" } } $('.js-work-strip[data-work-id=113549611]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":113549611,"title":"Adsorption of Phosphate and Nitrate Ions on Oxidic Substrates Prepared with a Variable-Charge Lithological Material","translated_title":"","metadata":{"abstract":"This work evaluates phosphate and nitrate ion adsorption from aqueous solutions on calcined adsorbent substrates of variable charge, prepared from three granulometric fractions of an oxidic lithological material. The adsorbent material was chemically characterized, and N2 gas adsorption (BET), X-ray diffraction, and DTA techniques were applied. The experimental conditions included the protonation of the beds with HCl and H2SO4 and the study of adsorption isotherms and kinetics. The lithological material was moderately acidic (pH 5) with very little solubility (electrical conductivity 0.013 dS m−1) and a low cation exchange capacity (53.67 cmol (+) kg−1). The protonation reaction was more efficient with HCl averaging 0.745 mmol versus 0.306 mmol with H2SO4. Likewise, the HCl-treated bed showed a better adsorption of PO4−3 ions (3.296 mg/100 g bed) compared to the H2SO4-treated bed (2.579 mg/100 g bed). The isotherms showed great affinity of the PO4−3 ions with the oxide surface, and ...","publisher":"MDPI AG","publication_name":"Water"},"translated_abstract":"This work evaluates phosphate and nitrate ion adsorption from aqueous solutions on calcined adsorbent substrates of variable charge, prepared from three granulometric fractions of an oxidic lithological material. The adsorbent material was chemically characterized, and N2 gas adsorption (BET), X-ray diffraction, and DTA techniques were applied. The experimental conditions included the protonation of the beds with HCl and H2SO4 and the study of adsorption isotherms and kinetics. The lithological material was moderately acidic (pH 5) with very little solubility (electrical conductivity 0.013 dS m−1) and a low cation exchange capacity (53.67 cmol (+) kg−1). The protonation reaction was more efficient with HCl averaging 0.745 mmol versus 0.306 mmol with H2SO4. Likewise, the HCl-treated bed showed a better adsorption of PO4−3 ions (3.296 mg/100 g bed) compared to the H2SO4-treated bed (2.579 mg/100 g bed). The isotherms showed great affinity of the PO4−3 ions with the oxide surface, and ...","internal_url":"https://www.academia.edu/113549611/Adsorption_of_Phosphate_and_Nitrate_Ions_on_Oxidic_Substrates_Prepared_with_a_Variable_Charge_Lithological_Material","translated_internal_url":"","created_at":"2024-01-15T12:51:32.909-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":36697270,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":110480457,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/110480457/thumbnails/1.jpg","file_name":"pdf.pdf","download_url":"https://www.academia.edu/attachments/110480457/download_file","bulk_download_file_name":"Adsorption_of_Phosphate_and_Nitrate_Ions.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/110480457/pdf-libre.pdf?1705355559=\u0026response-content-disposition=attachment%3B+filename%3DAdsorption_of_Phosphate_and_Nitrate_Ions.pdf\u0026Expires=1743230063\u0026Signature=Err4PQYlA31UZdOF4VFo-kP4OLZNFyHbkMtTj0y8NbWrINkCjYM7s5Xoxry7GUaCO3W2F-jh67e7LnJ58v1e-65vFjVlx1gOIfgMbjCvcU8Ep~4mYvErfhGlmwMTB5h9pyX2V85iA-nf60DxO3rOmvEBPmACYOTSoRj6APUgbRMeL0cDyk3x4rOvdoOB~-F4tYBH9XxfB4bUiuLMjpQSDmWhVlQdYo9V19OwVRkYNAyauGsR1O~g1lRLNTJYR8L-nwXSYQanSb3katbInybnT~N1jS-bfw97VAWQFRvdfvY6GwKrEhevb6eowqQz8Y1eoKQzZoQLuf4WBFqeeRIfbQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Adsorption_of_Phosphate_and_Nitrate_Ions_on_Oxidic_Substrates_Prepared_with_a_Variable_Charge_Lithological_Material","translated_slug":"","page_count":18,"language":"en","content_type":"Work","summary":"This work evaluates phosphate and nitrate ion adsorption from aqueous solutions on calcined adsorbent substrates of variable charge, prepared from three granulometric fractions of an oxidic lithological material. The adsorbent material was chemically characterized, and N2 gas adsorption (BET), X-ray diffraction, and DTA techniques were applied. The experimental conditions included the protonation of the beds with HCl and H2SO4 and the study of adsorption isotherms and kinetics. The lithological material was moderately acidic (pH 5) with very little solubility (electrical conductivity 0.013 dS m−1) and a low cation exchange capacity (53.67 cmol (+) kg−1). The protonation reaction was more efficient with HCl averaging 0.745 mmol versus 0.306 mmol with H2SO4. Likewise, the HCl-treated bed showed a better adsorption of PO4−3 ions (3.296 mg/100 g bed) compared to the H2SO4-treated bed (2.579 mg/100 g bed). The isotherms showed great affinity of the PO4−3 ions with the oxide surface, and ...","owner":{"id":36697270,"first_name":"Julio","middle_initials":null,"last_name":"Carrero","page_name":"JulioCarrero1","domain_name":"independent","created_at":"2015-10-21T12:09:01.815-07:00","display_name":"Julio Carrero","url":"https://independent.academia.edu/JulioCarrero1"},"attachments":[{"id":110480457,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/110480457/thumbnails/1.jpg","file_name":"pdf.pdf","download_url":"https://www.academia.edu/attachments/110480457/download_file","bulk_download_file_name":"Adsorption_of_Phosphate_and_Nitrate_Ions.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/110480457/pdf-libre.pdf?1705355559=\u0026response-content-disposition=attachment%3B+filename%3DAdsorption_of_Phosphate_and_Nitrate_Ions.pdf\u0026Expires=1743230063\u0026Signature=Err4PQYlA31UZdOF4VFo-kP4OLZNFyHbkMtTj0y8NbWrINkCjYM7s5Xoxry7GUaCO3W2F-jh67e7LnJ58v1e-65vFjVlx1gOIfgMbjCvcU8Ep~4mYvErfhGlmwMTB5h9pyX2V85iA-nf60DxO3rOmvEBPmACYOTSoRj6APUgbRMeL0cDyk3x4rOvdoOB~-F4tYBH9XxfB4bUiuLMjpQSDmWhVlQdYo9V19OwVRkYNAyauGsR1O~g1lRLNTJYR8L-nwXSYQanSb3katbInybnT~N1jS-bfw97VAWQFRvdfvY6GwKrEhevb6eowqQz8Y1eoKQzZoQLuf4WBFqeeRIfbQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"},{"id":110480456,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/110480456/thumbnails/1.jpg","file_name":"pdf.pdf","download_url":"https://www.academia.edu/attachments/110480456/download_file","bulk_download_file_name":"Adsorption_of_Phosphate_and_Nitrate_Ions.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/110480456/pdf-libre.pdf?1705355562=\u0026response-content-disposition=attachment%3B+filename%3DAdsorption_of_Phosphate_and_Nitrate_Ions.pdf\u0026Expires=1743230063\u0026Signature=hSw3cj6Q-UkerJ3eorQ456-hzTJRn9MAbItpwmmYLo8t-5yz9e8XwfXZF8~V5uiq-0XAOmKpc~5bFSbVz-tZ~cJJKLnT03y~bHa-2ctRCnsCRdMDvb2P2StMcG6tO81Fy~oESZM~V8x9Gk3DCZe9RBVLe8PNW6yHE0IRTluNl-VpMzMIhF7JWTK18OLyX7zn1H~n3kie-UiXS5F0TVM-XOfVMYXPYngxL3QmZnFbXGdDyfP3ef504bQLCzgIiiOkwomnjdJSVeE-IkHbTysHqI1pt~taIVxu~uPeKk0mFPu4czAyjHilDs95LH7Aye7A-8o9esBYYG7E~NdXuwwG~A__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":523,"name":"Chemistry","url":"https://www.academia.edu/Documents/in/Chemistry"},{"id":530,"name":"Inorganic Chemistry","url":"https://www.academia.edu/Documents/in/Inorganic_Chemistry"},{"id":2215,"name":"Water","url":"https://www.academia.edu/Documents/in/Water"},{"id":28235,"name":"Multidisciplinary","url":"https://www.academia.edu/Documents/in/Multidisciplinary"},{"id":39752,"name":"Adsorption","url":"https://www.academia.edu/Documents/in/Adsorption"},{"id":480226,"name":"Surface Charge","url":"https://www.academia.edu/Documents/in/Surface_Charge"},{"id":989646,"name":"Aqueous Solution","url":"https://www.academia.edu/Documents/in/Aqueous_Solution"},{"id":3306846,"name":"protonation","url":"https://www.academia.edu/Documents/in/protonation"}],"urls":[{"id":38604487,"url":"https://www.mdpi.com/2073-4441/14/16/2454/pdf"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") if (false) { Aedu.setUpFigureCarousel('profile-work-113549611-figures'); } }); </script> <div class="js-work-strip profile--work_container" data-work-id="113549610"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/113549610/The_Genomes_of_Two_Strains_of_Taenia_crassiceps_the_Animal_Model_for_the_Study_of_Human_Cysticercosis"><img alt="Research paper thumbnail of The Genomes of Two Strains of Taenia crassiceps the Animal Model for the Study of Human Cysticercosis" class="work-thumbnail" src="https://attachments.academia-assets.com/110480520/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/113549610/The_Genomes_of_Two_Strains_of_Taenia_crassiceps_the_Animal_Model_for_the_Study_of_Human_Cysticercosis">The Genomes of Two Strains of Taenia crassiceps the Animal Model for the Study of Human Cysticercosis</a></div><div class="wp-workCard_item"><span>Frontiers in Cellular and Infection Microbiology</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Human cysticercosis by Taenia solium is the major cause of neurological illness in countries of A...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Human cysticercosis by Taenia solium is the major cause of neurological illness in countries of Africa, Southeast Asia, and the Americas. Publication of four cestode genomes (T. solium, Echinococcus multilocularis, E. granulosus and Hymenolepis microstoma) in the last decade, marked the advent of novel approaches on the study of the host-parasite molecular crosstalk for cestode parasites of importance for human and animal health. Taenia crassiceps is another cestode parasite, closely related to T. solium, which has been used in numerous studies as an animal model for human cysticercosis. Therefore, characterization of the T. crassiceps genome will also contribute to the understanding of the human infection. Here, we report the genome of T. crassiceps WFU strain, reconstructed to a noncontiguous finished resolution and performed a genomic and differential expression comparison analysis against ORF strain. Both strain genomes were sequenced using Oxford Nanopore (MinION) and Illumina ...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="11ab74b0fcb795ac45f86de706fc3da6" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":110480520,"asset_id":113549610,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/110480520/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="113549610"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="113549610"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 113549610; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=113549610]").text(description); $(".js-view-count[data-work-id=113549610]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 113549610; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='113549610']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "11ab74b0fcb795ac45f86de706fc3da6" } } $('.js-work-strip[data-work-id=113549610]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":113549610,"title":"The Genomes of Two Strains of Taenia crassiceps the Animal Model for the Study of Human Cysticercosis","translated_title":"","metadata":{"abstract":"Human cysticercosis by Taenia solium is the major cause of neurological illness in countries of Africa, Southeast Asia, and the Americas. Publication of four cestode genomes (T. solium, Echinococcus multilocularis, E. granulosus and Hymenolepis microstoma) in the last decade, marked the advent of novel approaches on the study of the host-parasite molecular crosstalk for cestode parasites of importance for human and animal health. Taenia crassiceps is another cestode parasite, closely related to T. solium, which has been used in numerous studies as an animal model for human cysticercosis. Therefore, characterization of the T. crassiceps genome will also contribute to the understanding of the human infection. Here, we report the genome of T. crassiceps WFU strain, reconstructed to a noncontiguous finished resolution and performed a genomic and differential expression comparison analysis against ORF strain. Both strain genomes were sequenced using Oxford Nanopore (MinION) and Illumina ...","publisher":"Frontiers Media SA","ai_title_tag":"Genome Analysis of Taenia crassiceps Strains","publication_name":"Frontiers in Cellular and Infection Microbiology"},"translated_abstract":"Human cysticercosis by Taenia solium is the major cause of neurological illness in countries of Africa, Southeast Asia, and the Americas. Publication of four cestode genomes (T. solium, Echinococcus multilocularis, E. granulosus and Hymenolepis microstoma) in the last decade, marked the advent of novel approaches on the study of the host-parasite molecular crosstalk for cestode parasites of importance for human and animal health. Taenia crassiceps is another cestode parasite, closely related to T. solium, which has been used in numerous studies as an animal model for human cysticercosis. Therefore, characterization of the T. crassiceps genome will also contribute to the understanding of the human infection. Here, we report the genome of T. crassiceps WFU strain, reconstructed to a noncontiguous finished resolution and performed a genomic and differential expression comparison analysis against ORF strain. Both strain genomes were sequenced using Oxford Nanopore (MinION) and Illumina ...","internal_url":"https://www.academia.edu/113549610/The_Genomes_of_Two_Strains_of_Taenia_crassiceps_the_Animal_Model_for_the_Study_of_Human_Cysticercosis","translated_internal_url":"","created_at":"2024-01-15T12:51:32.779-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":36697270,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":110480520,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/110480520/thumbnails/1.jpg","file_name":"pdf.pdf","download_url":"https://www.academia.edu/attachments/110480520/download_file","bulk_download_file_name":"The_Genomes_of_Two_Strains_of_Taenia_cra.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/110480520/pdf-libre.pdf?1705355551=\u0026response-content-disposition=attachment%3B+filename%3DThe_Genomes_of_Two_Strains_of_Taenia_cra.pdf\u0026Expires=1743230063\u0026Signature=OR6uGi7KdO30BCljSus-0BdYDf3saPTJmem2ZmaoK~n4FjBADoFv7iVa~itMnTek18S1d5H0I~b0DY2yp4XQKdVfoiLyTltpVMSEc72GNlUbilhWweHEJq9JQJhZTF~6lt0py7k3kklJMHG0KQ-U8~jxi9A2SjcbYz14sNoel9amBsvONWjP5JbgORMuToRTplLxlpmETMOQ2xKqbXZe8lFIe3Ovg94Z4VvcBlFB7GAN5V7lTcFNiks45uZ5FKNbRYrJ16Kjpq3Sr-F0afh77s4qyH0zYZQyvPEgWtS-Bzv8e9MAJi63anWS~p7s8xVehm1zxMD0auZHG~uYQRir8w__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"The_Genomes_of_Two_Strains_of_Taenia_crassiceps_the_Animal_Model_for_the_Study_of_Human_Cysticercosis","translated_slug":"","page_count":12,"language":"en","content_type":"Work","summary":"Human cysticercosis by Taenia solium is the major cause of neurological illness in countries of Africa, Southeast Asia, and the Americas. 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Amoebiasis continues to be a public health problem, with increasing evidence of resistance to metronidazole. In this study, we assessed the effect of the alkaloid fraction of T. arborea root bark and the alkaloids ibogaine and voacangine on the viability and infectivity of Entamoeba histolytica trophozoites. Cultures were exposed to 0.1 – 10 µg/mL for 24, 48 and 72 h, and viability was then determined using a tetrazolium dye reduction assay and type of cellular death analyzed by flow cytometry. Results showed that the alkaloid fraction, but mainly ibogaine and voacangine alkaloids, exhibited potent dose-dependent anti-amoebic activity at 24 h post-exposure (IC50 4.5 and 8.1 µM, respectively), comparable to metronidazole (IC50 6.8 µM). 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This illness leads to one hundred thousand deaths per year worldwide, affecting mainly underdeveloped countries in areas with poor sanitary conditions. Throughout its life cycle or during the invasion of human tissues, the parasite is constantly subjected to stress conditions. In in vitro culture, this microaerophilic parasite can tolerate up to 50 μM oxygen concentrations; however, during invasion the parasite has to cope with the higher oxygen content found in the blood and well perfused tissues (60–130 μM) and with reactive oxygen and nitrogen species (ROS and NOS, respectively) derived from both the host (as a first line of defense against the infection) and from the oxygen and nitrogen detoxification systems within the parasite. Furthermore, notwithstanding that host body temperatures (36.5–39 ℃) observed du...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="113549608"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="113549608"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 113549608; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=113549608]").text(description); $(".js-view-count[data-work-id=113549608]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 113549608; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='113549608']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=113549608]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":113549608,"title":"Oxidative Stress and Heat Stress in Experimental Amoebic Liver Abscess","translated_title":"","metadata":{"abstract":"Several species belonging to the Genus Entamoeba can colonize the mouth or the human gut; however, only Entamoeba histolytica is pathogenic to the host, causing amoebiasis. 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Relationship with Virulence" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title">Role of Extracellular Traps Promoted by Intestinal Parasites. Relationship with Virulence</div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">NETosis is a form of programmed cell death in neutrophils characterized by the release of extrace...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">NETosis is a form of programmed cell death in neutrophils characterized by the release of extracellular DNA traps (NET) composed of DNA associated with histones, granule enzymes and antimicrobial peptides. This mechanism of innate immunity has been linked to cell defense against different pathogens. In case of bacteria, NETosis has been directly associated with virulence, suggesting that its formation could depend on the pathogen’s ability to cause tissue damage. 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Intracellular survival in macrophages within a membrane-bound vacuole (BcCV) that delays acidification and maturation into lysosomes is a hallmark of B. cenocepacia infection. Intracellular B. cenocepacia induce an inflammatory response leading to macrophage cell death by pyroptosis through the secretion of a bacterial deamidase that results in the activation of the pyrin inflammasome. However, how or whether infected macrophages can process and present B. cenocepacia antigens to activate T-cells has not been explored. Engulfed bacterial protein antigens are cleaved into small peptides in the late endosomal major histocompatibility class II complex (MHC) compartment (MIIC). Here, we demonstrate that BcCVs and MIICs have overlapping features and that interferongamma-activated macrophages infected with B. cenocepacia can process bacterial antigens for presentation by class II MHC molecules to CD4 + T-cells and by class I MHC molecules to CD8 + T-cells. Infected macrophages also release processed bacterial peptides into the extracellular medium, stabilizing empty class I MHC molecules of bystander cells. Together, we conclude that BcCVs acquire MIIC characteristics, supporting the notion that macrophages infected with B. cenocepacia contribute to establishing an adaptive immune response against the pathogen.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="cb12ed5de77311187a3ef402f0eaa270" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":110480454,"asset_id":113549606,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/110480454/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="113549606"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="113549606"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 113549606; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=113549606]").text(description); $(".js-view-count[data-work-id=113549606]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 113549606; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='113549606']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "cb12ed5de77311187a3ef402f0eaa270" } } $('.js-work-strip[data-work-id=113549606]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":113549606,"title":"Interferon-gamma-activated macrophages infected with Burkholderia cenocepacia process and present bacterial antigens to T-cells by class I and II major histocompatibility complex molecules","translated_title":"","metadata":{"publisher":"Informa UK Limited","grobid_abstract":"Burkholderia cenocepacia is an emerging opportunistic pathogen for people with cystic fibrosis and chronic granulomatous disease. 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Infected macrophages also release processed bacterial peptides into the extracellular medium, stabilizing empty class I MHC molecules of bystander cells. 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