GENEWIZ from Azenta | PCR Plus Sequencing CRISPR Analysis Package

<!DOCTYPE html>    <html lang="en">  <head> <title>GENEWIZ from Azenta | PCR Plus Sequencing CRISPR Analysis Package</title>  <meta charset="utf-8"> <meta http-equiv="X-UA-Compatible" content="IE=edge,chrome=1"> <meta name="viewport" content="width=device-width, initial-scale=1.0"> <meta name="description" content="GENEWIZ offers a PCR Sequencing CRISPR Analysis package to help genomic-level researchers verify the successful edit of their regions of interest."> <meta name="keywords" content="genewiz, azenta life sciences, CRISPR, CRISPR/Cas9, guide RNA, gRNA, sgRNA, single guide RNA, Genome engineering, Genome editing, Gene editing, Gene knockout, Gene KO, Gene knock-in, Gene therapy, transgenic animal generation, TALEN, CRISPR associated protein 9, Cas9 nuclease, Cas9-gRNA complex, including amplicon selection, snp discovery, snp discovery sequencing, mutation, pcr amplification, single nucleotide polymorphisms, mutation analysis, optimized assays, exon resequencing, assay optimization, purified genomic dna templates, high-throughput facility, throughput pcr amplification, capillary electrophoresis, amplified using optimized assays, extract genomic, pcr assays targeted, non-sense mutation, snp discovery assays, high throughput pcr amplification"> <meta name="author" content="">  <link rel="shortcut icon" href="favicon.png">   <link rel="stylesheet" href="/assets/plugins/bootstrap/css/bootstrap.min.css"> <link rel="stylesheet" href="/assets/css/style.css?v=1"> <link rel="stylesheet" href="/assets/css/custom.css?v=1"> <link rel="canonical" href="https://www.genewiz.com/Public/Services/Molecular-Genetics/PCR-Plus-Sequencing-CRISPR-Analysis-Package/" /> <link rel="alternate" hreflang="x-default" href="https://www.genewiz.com/Public/Services/Molecular-Genetics/PCR-Plus-Sequencing-CRISPR-Analysis-Package" /> <link rel="alternate" hreflang="en-us" href="https://www.genewiz.com/Public/Services/Molecular-Genetics/PCR-Plus-Sequencing-CRISPR-Analysis-Package" /> <link rel="alternate" hreflang="en-us" href="https://www.genewiz.com/en/Public/Services/Molecular-Genetics/PCR-Plus-Sequencing-CRISPR-Analysis-Package" /> <link rel="alternate" hreflang="en-GB" href="https://www.genewiz.com/en-GB/Public/Services/Molecular-Genetics/PCR-Plus-Sequencing-CRISPR-Analysis-Package" /> <script type="text/javascript" src="/sitecore/shell/controls/lib/prototype/prototype.js"></script> <script type="text/javascript" src="/assets/plugins/jquery/jquery.min.js"></script> <script type="text/javascript"> var jQuery = $; //if (window.location.search.indexOf("sc_mode=edit") > 0) { jQuery.noConflict(); //} </script> <script type="text/javascript" src="/assets/plugins/bootstrap/js/bootstrap.min.js"></script> <script type="text/javascript" src="/assets/plugins/back-to-top.js"></script> <script type="text/javascript" src="/assets/js/app.js"></script>   <script> (function (w, d, s, l, i) { w[l] = w[l] || []; w[l].push( { 'gtm.start': new Date().getTime(), event: 'gtm.js' } ); var f = d.getElementsByTagName(s)[0], j = d.createElement(s), dl = l != 'dataLayer' ? 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efficiency of CRISPR-mediated genome editing. Our CRISPR Sanger sequencing service is designed to verify the successful edit of your region of interest, starting with cells or genomic DNA.</p> <a name="solution"></a> <br /> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://clims4.genewiz.com/DynamicForm/CreateView?lobId=10&serviceId=1022" target="_blank"><button>Request Quote</button>   </a></div> <div class="ngs-content-box" id="ngs-content-box-right"><a rel="noopener noreferrer" href="https://cdn2.hubspot.net/hubfs/3478602/Sell Sheet Collateral Library/NGS/Sell Sheets + Brochures/NGS_Overview Sell Sheet_032219.pdf" target="_blank"></a><a rel="noopener noreferrer" href="https://web.genewiz.com/hubfs/NGS/NGS Content/NGS Brochure/NGS_Overview_Digital_Brochure_Feb26_2018_no_crops.pdf" target="_blank"> </a><a rel="noopener noreferrer" rel="noopener noreferrer" href="https://hubs.ly/Q01l9_fp0" target="_blank"><img src="/-/media/Images/12037-D_25-CRISPR_WebBanRight.ashx?la=en&hash=ACDD28323DF8B5139F2702DC82A99010ED1BD41C" alt="CRISPR Promo" /> </a> </div> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <link href="https://fonts.googleapis.com/css?family=Roboto:300,400,500,700" rel="stylesheet" /> <style type="text/css"> .covid-banner { width: 1170px; height: auto; margin: 0 auto; background-image: url("/-/media/11037-D_Sanger-Troubleshoot_WebBanFull-Med_Background.ashx"); background-size: cover; background-position: center; text-align: left; padding: 0px 0 0px 0; } .covid-content { text-align: center; } .covid-box h3 { color: #ffffff; text-transform: none; letter-spacing: 1px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 450; line-height: 125%; font-size: 35px; margin: 0; padding-bottom: 15px; padding-top: 25px; } .covid-box h4 { color: #ffffff; text-transform: none; letter-spacing: 1px; line-height: 30px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 35px; margin: 0; margin-bottom: 5px; } .covid-box p { color: #000000; text-transform: none; letter-spacing: 1px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 15px; margin: 0; margin-bottom: 5px; } .covid-box button { padding: 8px 19px; color: #826ec3; letter-spacing: 1px; background: #ffffff; border: 2px solid #ffffff; border-radius: 0px; transition: all .2s ease-in-out; font-size: 15px } .covid-box button:hover { cursor: pointer; transform: scale(1.1); } .covid-box button:focus { outline: 0; } @media only screen and (max-width: 1170px) { .covid-banner { width: 100%; height: auto; } .covid-content { width: 90%; margin: 0 auto; } } </style> <br /> <div class="covid-banner"> <div class="covid-content"> <div class="covid-box"> <br /> <h3>Troubleshooting DNA Templates<br /> with Sanger Sequencing</h3> <a href="https://www.azenta.com/learning-center/blog/troubleshooting-dna-templates-sanger-sequencing?utm_campaign=2024_05_GEN_SANGER%3A%20Trouble%20Shooting%20Sanger%20Sequencing%20Blog&utm_source=EU%20CRISPR%20Analysis%20Page&utm_medium=Full%20Banner%20Ad" target="_blank"> <br /> <button> READ BLOG</button> <br /> </a> <p> </p> </div> </div> </div> <p> </p> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> @import url('https://fonts.googleapis.com/css?family=Roboto:300,400,500,700'); body { margin: 0; border: 0; padding: 0; } .sanger-plasmid-prep-banner { width: 100%; height: 120px; background: #62b864; text-align: left; margin-top: -50px; } .sanger-plasmid-prep-banner-content { width: 1170px; margin: 0 auto; } .sanger-plasmid-prep-box { display: inline-block; vertical-align: middle; } .sanger-plasmid-prep-box h2 { font-family: 'Roboto', sans-serif; color: #fff; text-transform: none; letter-spacing: 1px; padding-top: 45px; border-bottom: none; font-size: 20px; font-weight: 700; } #sanger-plasmid-prep-box-image { width: 10%; text-align: right; } #sanger-plasmid-prep-box-left { width: 65%; margin-left: 15px; } #sanger-plasmid-prep-box-right { text-align: right; width: 15%; } #sanger-plasmid-prep-box-left p { font-family: 'Roboto', sans-serif; font-size: 14px; font-weight: 500; letter-spacing: 2px; color: #fff; } #sanger-plasmid-prep-box-right button { padding: 15px 25px 15px 25px; color: #0067B1; font-size: 14px; font-weight: bold; letter-spacing: 1px; background: #fff; border: none; transition: all .2s ease-in-out; } #sanger-plasmid-prep-box-right button:hover { cursor: pointer; transform: scale(1.1); } #sanger-plasmid-prep-box-right button:focus { outline: 0; } </style> <div class="container text-left margin-bottom-30"> <h2 style="text-align: center;">CRISPR MUTATION ANALYSIS</h2> <p>Our Mutation Analysis service includes PCR optimization, amplicon production and purification, and Sanger sequencing for your CRISPR regions of interest. Whether you are confirming that a mutation has occurred or precisely determining the genotype and specific allelic event, leveraging our expertise ensures that your genome editing functions efficiently.</p> <p>For CRISPR experiments, our experienced scientists can interrogate your specified on-target regions of interest. Azenta can also confirm off-target effects of the RNA-guided Cas9 nuclease<sup>1</sup>. Simply identify your target regions and let our Sanger experts handle the rest.</p> <p style="font-size: 11px;">1 Nucleic Acids Research (2014) doi: 10.1093/nar/gku402. CRISPR/Cas9 systems have off-target activity with insertions or deletions between target DNA and guide RNA sequences. Lin, Y, et. Al.  </p> </div> </div> <script type="text/javascript" src="/assets/plugins/counter/jquery.counterup.min.js"></script> <script language="javascript" type="text/javascript"> jQuery(document).ready(function ($) { $(".row .category").find("img").addClass("img-responsive"); }); </script> <div class='Component-Frame pull-center Multi-column-TextBullet-Layout Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10'> <div class="container"> <div class="headline-center"> <h2>GENEWIZ PCR PLUS SEQUENCING CRISPR ANALYSIS SERVICES</h2> </div> <div class="headline-left"> </div> <div class="row category"> <div class="col-md-6 col-sm-6"> <div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> Extraction of genomic DNA from single-isolate cell samples.<br><br> </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> Amplicon selection and primer design for on- and off-target regions. </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> Assay development and optimization. </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> PCR amplification of genomic DNA. </div> </div> </div> <div class="col-md-6 col-sm-6"> <div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> High-throughput PCR purification and bi-directional DNA sequencing. </div> </div><div 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genomic DNA templates or extract it from supplied sources. </div> </div> </div> <div class="col-md-6 col-sm-6"> <div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> <p><strong>High-Throughput Processing</strong>: Target regions are amplified using optimized assays; amplicons are purified and sequenced by capillary electrophoresis in our best-in-class facility. </p> </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> <strong>Accurate Data Analysis:</strong> Mutations are identified with both automated and manual analysis to ensure accuracy. </div> </div> </div> </div> </div> </div><div class="headline-center Rich-Text-Edit-Area"> <link href="https://fonts.googleapis.com/css?family=Roboto:300,400,500,700" 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