HIF-1 alpha/HIF1A: Antibodies

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Systems brands. Our HIF-1 alpha/HIF1A antibodies have been cited in 2491 publications with 63 5-star customer reviews. Browse our catalog for anti-HIF-1 alpha/HIF1A monoclonal, polyclonal, and recombinant monoclonal antibodies. These antibodies are reactive in target species including Human, Mouse, Rat, Canine, Bovine, and more. Our HIF-1 alpha/HIF1A antibodies have been validated for use in several research applications: Simple Western, Knockout Validated, Knockdown Validated, Dual RNAscope ISH-IHC, Immunohistochemistry, and more. Choose the anti-HIF-1 alpha/HIF1A antibody best suited for your research needs by viewing the technical details, images, citations, reviews, and more. Each HIF-1 alpha/HIF1A antibody is fully covered by Bio-Techne’s 100% guarantee. <br/><br/>Bio-Techne offers a comprehensive range of other <a href="https://www.bio-techne.com/t/hif-1-alpha-hif1a">HIF-1 alpha/HIF1A-related products</a> including <a href="https://www.bio-techne.com/t/hif-1-alpha-hif1a/elisa-kits">HIF-1 alpha/HIF1A ELISA Kits</a>, <a href="https://www.bio-techne.com/t/hif-1-alpha-hif1a/proteins-enzymes">HIF-1 alpha/HIF1A Proteins and Enzymes</a>, and more.</p>"> <div class="data-display"> <p>Bio-Techne offers high quality HIF-1 alpha/HIF1A antibodies from our Novus Biologicals and R&D Systems brands. Our HIF-1 alpha/HIF1A antibodies have been cited in 2491 publications with 63 5-star customer reviews. Browse our catalog for anti-HIF-1 alpha/HIF1A monoclonal, polyclonal, and recombinant monoclonal antibodies. These antibodies are reactive in target species including Human, Mouse, Rat, Canine, Bovine, and more. Our HIF-1 alpha/HIF1A antibodies have been validated for use in several research applications: Simple Western, Knockout Validated, Knockdown Validated, Dual RNAscope ISH-IHC, Immunohistochemistry, and more. Choose the anti-HIF-1 alpha/HIF1A antibody best suited for your research needs by viewing the technical details, images, citations, reviews, and more. Each HIF-1 alpha/HIF1A antibody is fully covered by Bio-Techne’s 100% guarantee. <br><br>Bio-Techne offers a comprehensive range of other <a href="https://www.bio-techne.com/t/hif-1-alpha-hif1a">HIF-1 alpha/HIF1A-related products</a> including <a href="https://www.bio-techne.com/t/hif-1-alpha-hif1a/elisa-kits">HIF-1 alpha/HIF1A ELISA Kits</a>, <a href="https://www.bio-techne.com/t/hif-1-alpha-hif1a/proteins-enzymes">HIF-1 alpha/HIF1A Proteins and Enzymes</a>, and more.</p> </div> <a href="#" class="show-more-btn">Show More</a> </div> <p> <span class="search_num_results" data-search_results="447">447 results</span> for "<span class="search_term_text">HIF-1 alpha/HIF1A Primary Antibodies</span>" in <strong id="search_category">Products</strong> </p> </div> <div class="row"> <div class="col-12 col-sm-6 text-sm-right my-2"> <button id="sort-filter" class="border rounded btn w-100 text-left" type="button">Done</button> </div> </div> </div> <div class="row"> <div class="col-md-12 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<span class="radio_circle_inner"></span> </span> <span data-facet="Monoclonal Antibodies (170)" data-facet_lower="monoclonal antibodies" class="search_facet_name text-break sub_category"> Monoclonal Antibodies <span class="facet_count">(170)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?sub_category=Polyclonal Antibodies" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="Polyclonal Antibodies (212)" data-facet_lower="polyclonal antibodies" class="search_facet_name text-break sub_category"> Polyclonal Antibodies <span class="facet_count">(212)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?sub_category=Recombinant Monoclonal Antibodies" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="Recombinant Monoclonal Antibodies (63)" data-facet_lower="recombinant monoclonal antibodies" class="search_facet_name text-break sub_category"> Recombinant Monoclonal Antibodies <span class="facet_count">(63)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?sub_category=Antibody Packs" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="Antibody Packs (2)" data-facet_lower="antibody packs" class="search_facet_name text-break sub_category"> Antibody Packs <span class="facet_count">(2)</span> </span> </a> </div> </div> </div> </div> <div class="search_facet_category_block"> <div class="dropdown-container position-relative"> <button class="btn btn-secondary dropdown-toggle horizontal-dropdown" type="button" id="dropdown-menu-button-species" data-toggle="dropdown" aria-haspopup="true" aria-expanded="false"> Species </button> <div class="dropdown-menu" aria-labelledby="dropdown-menu-button-species"> <div class="facet search_facet_search_category facet_list p-1"> <input type="text" class="form-control w-100 my-2 facet_category_search species" data-facet_title="species" placeholder="Search Species..."/> </div> <div class="facets_wrapper"> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?species=Human" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Human (447)" data-facet_lower="human" class="search_facet_name text-break species"> Human <span class="facet_count">(447)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?species=Mouse" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Mouse (238)" data-facet_lower="mouse" class="search_facet_name text-break species"> Mouse <span class="facet_count">(238)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?species=Rat" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Rat (179)" data-facet_lower="rat" class="search_facet_name text-break species"> Rat <span class="facet_count">(179)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?species=Canine" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Canine (147)" data-facet_lower="canine" class="search_facet_name text-break species"> Canine <span class="facet_count">(147)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?species=Primate" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Primate (120)" data-facet_lower="primate" class="search_facet_name text-break species"> Primate <span class="facet_count">(120)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?species=Bovine" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Bovine (141)" data-facet_lower="bovine" class="search_facet_name text-break species"> Bovine <span class="facet_count">(141)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?species=Rabbit" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Rabbit (119)" data-facet_lower="rabbit" class="search_facet_name text-break species"> Rabbit <span class="facet_count">(119)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?species=Porcine" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Porcine (119)" data-facet_lower="porcine" class="search_facet_name text-break species"> Porcine <span class="facet_count">(119)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?species=Zebrafish" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Zebrafish (62)" data-facet_lower="zebrafish" class="search_facet_name text-break species"> Zebrafish <span class="facet_count">(62)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?species=Monkey" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Monkey (59)" data-facet_lower="monkey" class="search_facet_name text-break species"> Monkey <span class="facet_count">(59)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?species=Xenopus" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Xenopus (58)" data-facet_lower="xenopus" class="search_facet_name text-break species"> Xenopus <span class="facet_count">(58)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?species=Ferret" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Ferret (56)" data-facet_lower="ferret" class="search_facet_name text-break species"> Ferret <span class="facet_count">(56)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?species=Sheep" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Sheep (56)" data-facet_lower="sheep" class="search_facet_name text-break species"> Sheep <span class="facet_count">(56)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?species=Goat" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Goat (34)" data-facet_lower="goat" class="search_facet_name text-break species"> Goat <span class="facet_count">(34)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?species=Fish - Danio rerio (Zebrafish)" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Fish - Danio rerio (Zebrafish) (2)" data-facet_lower="fish - danio rerio (zebrafish)" class="search_facet_name text-break species"> Fish - Danio rerio (Zebrafish) <span class="facet_count">(2)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?species=Frog - Xenopus (African Clawed Frog)" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Frog - Xenopus (African Clawed Frog) (2)" data-facet_lower="frog - xenopus (african clawed frog)" class="search_facet_name text-break species"> Frog - Xenopus (African Clawed Frog) <span class="facet_count">(2)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?species=Ovine" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Ovine (2)" data-facet_lower="ovine" class="search_facet_name text-break species"> Ovine <span class="facet_count">(2)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?species=Fish" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Fish (33)" data-facet_lower="fish" class="search_facet_name text-break species"> Fish <span class="facet_count">(33)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?species=Hamster" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Hamster (33)" data-facet_lower="hamster" class="search_facet_name text-break species"> Hamster <span class="facet_count">(33)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?species=Feline" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Feline (29)" data-facet_lower="feline" class="search_facet_name text-break species"> Feline <span class="facet_count">(29)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?species=Guinea Pig" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Guinea Pig (29)" data-facet_lower="guinea pig" class="search_facet_name text-break species"> Guinea Pig <span class="facet_count">(29)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?species=Avian" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Avian (27)" data-facet_lower="avian" class="search_facet_name text-break species"> Avian <span class="facet_count">(27)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?species=Avian - Chicken" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Avian - Chicken (1)" data-facet_lower="avian - chicken" class="search_facet_name text-break species"> Avian - Chicken <span class="facet_count">(1)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?species=Chicken" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Chicken (1)" data-facet_lower="chicken" class="search_facet_name text-break species"> Chicken <span class="facet_count">(1)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?species=Hamster - Cricetulus (Chinese Hamster)" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Hamster - Cricetulus (Chinese Hamster) (1)" data-facet_lower="hamster - cricetulus (chinese hamster)" class="search_facet_name text-break species"> Hamster - Cricetulus (Chinese Hamster) <span class="facet_count">(1)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?species=Moth - Trichoplusia ni (Cabbage Looper)" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Moth - Trichoplusia ni (Cabbage Looper) (1)" data-facet_lower="moth - trichoplusia ni (cabbage looper)" class="search_facet_name text-break species"> Moth - Trichoplusia ni (Cabbage Looper) <span class="facet_count">(1)</span> </span> </a> </div> </div> </div> </div> </div> <div class="search_facet_category_block"> <div class="dropdown-container position-relative"> <button class="btn btn-secondary dropdown-toggle horizontal-dropdown" type="button" id="dropdown-menu-button-applications" data-toggle="dropdown" aria-haspopup="true" aria-expanded="false"> Application </button> <div class="dropdown-menu" aria-labelledby="dropdown-menu-button-applications"> <div class="facet search_facet_search_category facet_list p-1"> <input type="text" class="form-control w-100 my-2 facet_category_search applications" data-facet_title="applications" placeholder="Search Application..."/> </div> <div class="facets_wrapper"> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?applications=Simple Western" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Simple Western (215)" data-facet_lower="simple western" class="search_facet_name text-break applications"> Simple Western <span class="facet_count">(215)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?applications=Flow Cytometry" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Flow Cytometry (146)" data-facet_lower="flow cytometry" class="search_facet_name text-break applications"> Flow Cytometry <span class="facet_count">(146)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?applications=Western Blot" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Western Blot (417)" data-facet_lower="western blot" class="search_facet_name text-break applications"> Western Blot <span class="facet_count">(417)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?applications=Immunocytochemistry/ Immunofluorescence" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Immunocytochemistry/ Immunofluorescence (242)" data-facet_lower="immunocytochemistry/ immunofluorescence" class="search_facet_name text-break applications"> Immunocytochemistry/ Immunofluorescence <span class="facet_count">(242)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?applications=Immunohistochemistry" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Immunohistochemistry (256)" data-facet_lower="immunohistochemistry" class="search_facet_name text-break applications"> Immunohistochemistry <span class="facet_count">(256)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?applications=Knockout Validated" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Knockout Validated (151)" data-facet_lower="knockout validated" class="search_facet_name text-break applications"> Knockout Validated <span class="facet_count">(151)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?applications=Immunoprecipitation" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Immunoprecipitation (180)" data-facet_lower="immunoprecipitation" class="search_facet_name text-break applications"> Immunoprecipitation <span class="facet_count">(180)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?applications=Chromatin Immunoprecipitation" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Chromatin Immunoprecipitation (121)" data-facet_lower="chromatin immunoprecipitation" class="search_facet_name text-break applications"> Chromatin Immunoprecipitation <span class="facet_count">(121)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?applications=Knockdown Validated" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Knockdown Validated (88)" data-facet_lower="knockdown validated" class="search_facet_name text-break applications"> Knockdown Validated <span class="facet_count">(88)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?applications=ELISA" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="ELISA (87)" data-facet_lower="elisa" class="search_facet_name text-break applications"> ELISA <span class="facet_count">(87)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?applications=Functional Assay" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Functional Assay (32)" data-facet_lower="functional assay" class="search_facet_name text-break applications"> Functional Assay <span class="facet_count">(32)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?applications=Gel Shift" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Gel Shift (114)" data-facet_lower="gel shift" class="search_facet_name text-break applications"> Gel Shift <span class="facet_count">(114)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?applications=Block/Neutralize" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Block/Neutralize (3)" data-facet_lower="block/neutralize" class="search_facet_name text-break applications"> Block/Neutralize <span class="facet_count">(3)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?applications=Dual RNAscope ISH-IHC" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Dual RNAscope ISH-IHC (29)" data-facet_lower="dual rnascope ish-ihc" class="search_facet_name text-break applications"> Dual RNAscope ISH-IHC <span class="facet_count">(29)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?applications=N/A" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="N/A (29)" data-facet_lower="n/a" class="search_facet_name text-break applications"> N/A <span class="facet_count">(29)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?applications=CyTOF-ready" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="CyTOF-ready (17)" data-facet_lower="cytof-ready" class="search_facet_name text-break applications"> CyTOF-ready <span class="facet_count">(17)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?applications=SDS-PAGE" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="SDS-PAGE (1)" data-facet_lower="sds-page" class="search_facet_name text-break applications"> SDS-PAGE <span class="facet_count">(1)</span> </span> </a> </div> </div> </div> </div> </div> <div class="search_facet_category_block"> <div class="dropdown-container position-relative"> <button class="btn btn-secondary dropdown-toggle horizontal-dropdown" type="button" id="dropdown-menu-button-conjugate_form" data-toggle="dropdown" aria-haspopup="true" aria-expanded="false"> Conjugates </button> <div class="dropdown-menu" aria-labelledby="dropdown-menu-button-conjugate_form"> <div class="facet search_facet_search_category facet_list p-1"> <input type="text" class="form-control w-100 my-2 facet_category_search conjugate_form" data-facet_title="conjugate_form" placeholder="Search Conjugates..."/> </div> <div class="facets_wrapper"> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?conjugate_form=Unconjugated" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="Unconjugated (29)" data-facet_lower="unconjugated" class="search_facet_name text-break conjugate_form"> Unconjugated <span class="facet_count">(29)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?conjugate_form=Alexa Fluor 350" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="Alexa Fluor 350 (15)" data-facet_lower="alexa fluor 350" class="search_facet_name text-break conjugate_form"> Alexa Fluor 350 <span class="facet_count">(15)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?conjugate_form=Alexa Fluor 405" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="Alexa Fluor 405 (15)" data-facet_lower="alexa fluor 405" class="search_facet_name text-break conjugate_form"> Alexa Fluor 405 <span class="facet_count">(15)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?conjugate_form=Alexa Fluor 488" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="Alexa Fluor 488 (15)" data-facet_lower="alexa fluor 488" class="search_facet_name text-break conjugate_form"> Alexa Fluor 488 <span class="facet_count">(15)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?conjugate_form=Alexa Fluor 594" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="Alexa Fluor 594 (15)" data-facet_lower="alexa fluor 594" class="search_facet_name text-break conjugate_form"> Alexa Fluor 594 <span class="facet_count">(15)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?conjugate_form=Alexa Fluor 647" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="Alexa Fluor 647 (15)" data-facet_lower="alexa fluor 647" class="search_facet_name text-break conjugate_form"> Alexa Fluor 647 <span class="facet_count">(15)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?conjugate_form=Alexa Fluor 700" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="Alexa Fluor 700 (15)" data-facet_lower="alexa fluor 700" class="search_facet_name text-break conjugate_form"> Alexa Fluor 700 <span class="facet_count">(15)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?conjugate_form=Alexa Fluor 750" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="Alexa Fluor 750 (15)" data-facet_lower="alexa fluor 750" class="search_facet_name text-break conjugate_form"> Alexa Fluor 750 <span class="facet_count">(15)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?conjugate_form=Allophycocyanin" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="Allophycocyanin (14)" data-facet_lower="allophycocyanin" class="search_facet_name text-break conjugate_form"> Allophycocyanin <span class="facet_count">(14)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?conjugate_form=Fluorescein" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="Fluorescein (1)" data-facet_lower="fluorescein" class="search_facet_name text-break conjugate_form"> Fluorescein <span class="facet_count">(1)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?conjugate_form=PerCP (Peridinin-chlorophyll Protein Complex)" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="PerCP (Peridinin-chlorophyll Protein Complex) (1)" data-facet_lower="percp (peridinin-chlorophyll protein complex)" class="search_facet_name text-break conjugate_form"> PerCP (Peridinin-chlorophyll Protein Complex) <span class="facet_count">(1)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?conjugate_form=Phycoerythrin" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="Phycoerythrin (1)" data-facet_lower="phycoerythrin" class="search_facet_name text-break conjugate_form"> Phycoerythrin <span class="facet_count">(1)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?conjugate_form=Alexa Fluor 532" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="Alexa Fluor 532 (15)" data-facet_lower="alexa fluor 532" class="search_facet_name text-break conjugate_form"> Alexa Fluor 532 <span class="facet_count">(15)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?conjugate_form=Biotin" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="Biotin (15)" data-facet_lower="biotin" class="search_facet_name text-break conjugate_form"> Biotin <span class="facet_count">(15)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?conjugate_form=DyLight 350" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="DyLight 350 (15)" data-facet_lower="dylight 350" class="search_facet_name text-break conjugate_form"> DyLight 350 <span class="facet_count">(15)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?conjugate_form=DyLight 405" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="DyLight 405 (15)" data-facet_lower="dylight 405" class="search_facet_name text-break conjugate_form"> DyLight 405 <span class="facet_count">(15)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?conjugate_form=DyLight 488" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="DyLight 488 (15)" data-facet_lower="dylight 488" class="search_facet_name text-break conjugate_form"> DyLight 488 <span class="facet_count">(15)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?conjugate_form=DyLight 550" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="DyLight 550 (15)" data-facet_lower="dylight 550" class="search_facet_name text-break conjugate_form"> DyLight 550 <span class="facet_count">(15)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?conjugate_form=DyLight 594" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="DyLight 594 (15)" data-facet_lower="dylight 594" class="search_facet_name text-break conjugate_form"> DyLight 594 <span class="facet_count">(15)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?conjugate_form=DyLight 650" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="DyLight 650 (15)" data-facet_lower="dylight 650" class="search_facet_name text-break conjugate_form"> DyLight 650 <span class="facet_count">(15)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?conjugate_form=DyLight 680" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="DyLight 680 (15)" data-facet_lower="dylight 680" class="search_facet_name text-break conjugate_form"> DyLight 680 <span class="facet_count">(15)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?conjugate_form=DyLight 755" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="DyLight 755 (15)" data-facet_lower="dylight 755" class="search_facet_name text-break conjugate_form"> DyLight 755 <span class="facet_count">(15)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?conjugate_form=HRP" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="HRP (15)" data-facet_lower="hrp" class="search_facet_name text-break conjugate_form"> HRP <span class="facet_count">(15)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?conjugate_form=mFluor Violet 450 SE" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="mFluor Violet 450 SE (15)" data-facet_lower="mfluor violet 450 se" class="search_facet_name text-break conjugate_form"> mFluor Violet 450 SE <span class="facet_count">(15)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?conjugate_form=mFluor Violet 500 SE" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="mFluor Violet 500 SE (15)" data-facet_lower="mfluor violet 500 se" class="search_facet_name text-break conjugate_form"> mFluor Violet 500 SE <span class="facet_count">(15)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?conjugate_form=mFluor Violet 610 SE" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="mFluor Violet 610 SE (15)" data-facet_lower="mfluor violet 610 se" class="search_facet_name text-break conjugate_form"> mFluor Violet 610 SE <span class="facet_count">(15)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?conjugate_form=CoraFluor 1" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="CoraFluor 1 (14)" data-facet_lower="corafluor 1" class="search_facet_name text-break conjugate_form"> CoraFluor 1 <span class="facet_count">(14)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?conjugate_form=Janelia Fluor 549" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="Janelia Fluor 549 (14)" data-facet_lower="janelia fluor 549" class="search_facet_name text-break conjugate_form"> Janelia Fluor 549 <span class="facet_count">(14)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?conjugate_form=Janelia Fluor 646" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="Janelia Fluor 646 (14)" data-facet_lower="janelia fluor 646" class="search_facet_name text-break conjugate_form"> Janelia Fluor 646 <span class="facet_count">(14)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?conjugate_form=PE" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="PE (13)" data-facet_lower="pe" class="search_facet_name text-break conjugate_form"> PE <span class="facet_count">(13)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?conjugate_form=PerCP" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="PerCP (13)" data-facet_lower="percp" class="search_facet_name text-break conjugate_form"> PerCP <span class="facet_count">(13)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?conjugate_form=FITC" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="FITC (9)" data-facet_lower="fitc" class="search_facet_name text-break conjugate_form"> FITC <span class="facet_count">(9)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?conjugate_form=Janelia Fluor 525" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="Janelia Fluor 525 (2)" data-facet_lower="janelia fluor 525" class="search_facet_name text-break conjugate_form"> Janelia Fluor 525 <span class="facet_count">(2)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?conjugate_form=Janelia Fluor 585" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="Janelia Fluor 585 (2)" data-facet_lower="janelia fluor 585" class="search_facet_name text-break conjugate_form"> Janelia Fluor 585 <span class="facet_count">(2)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?conjugate_form=Allophycocyanin/Cy7" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="Allophycocyanin/Cy7 (1)" data-facet_lower="allophycocyanin/cy7" class="search_facet_name text-break conjugate_form"> Allophycocyanin/Cy7 <span class="facet_count">(1)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?conjugate_form=Janelia Fluor 635" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="Janelia Fluor 635 (1)" data-facet_lower="janelia fluor 635" class="search_facet_name text-break conjugate_form"> Janelia Fluor 635 <span class="facet_count">(1)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?conjugate_form=PE/Atto594" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="PE/Atto594 (1)" data-facet_lower="pe/atto594" class="search_facet_name text-break conjugate_form"> PE/Atto594 <span class="facet_count">(1)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?conjugate_form=PE/Cy5.5" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="PE/Cy5.5 (1)" data-facet_lower="pe/cy5.5" class="search_facet_name text-break conjugate_form"> PE/Cy5.5 <span class="facet_count">(1)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?conjugate_form=PE/Cy7" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="PE/Cy7 (1)" data-facet_lower="pe/cy7" class="search_facet_name text-break conjugate_form"> PE/Cy7 <span class="facet_count">(1)</span> </span> </a> </div> </div> </div> </div> </div> <div class="search_facet_category_block"> <div class="dropdown-container position-relative"> <button class="btn btn-secondary dropdown-toggle horizontal-dropdown" type="button" id="dropdown-menu-button-clonality" data-toggle="dropdown" aria-haspopup="true" aria-expanded="false"> Clonality </button> <div class="dropdown-menu" aria-labelledby="dropdown-menu-button-clonality"> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?clonality=Monoclonal" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="Monoclonal (233)" data-facet_lower="monoclonal" class="search_facet_name text-break clonality"> Monoclonal <span class="facet_count">(233)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?clonality=Polyclonal" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="Polyclonal (212)" data-facet_lower="polyclonal" class="search_facet_name text-break clonality"> Polyclonal <span class="facet_count">(212)</span> </span> </a> </div> </div> </div> </div> <div class="search_facet_category_block"> <div class="dropdown-container position-relative"> <button class="btn btn-secondary dropdown-toggle horizontal-dropdown" type="button" id="dropdown-menu-button-host" data-toggle="dropdown" aria-haspopup="true" aria-expanded="false"> Host Species </button> <div class="dropdown-menu" aria-labelledby="dropdown-menu-button-host"> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?host=Mouse" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="Mouse (170)" data-facet_lower="mouse" class="search_facet_name text-break host"> Mouse <span class="facet_count">(170)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?host=Rabbit" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="Rabbit (274)" data-facet_lower="rabbit" class="search_facet_name text-break host"> Rabbit <span class="facet_count">(274)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?host=Goat" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="Goat (1)" data-facet_lower="goat" class="search_facet_name text-break host"> Goat <span class="facet_count">(1)</span> </span> </a> </div> </div> </div> </div> <div class="search_facet_category_block"> <div class="dropdown-container position-relative"> <button class="btn btn-secondary dropdown-toggle more-facet" type="button" id="dropdown-menu-button-isotype" data-toggle="dropdown" aria-haspopup="true" aria-expanded="false"> Isotype </button> <div class="dropdown-menu" aria-labelledby="dropdown-menu-button-isotype"> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?isotype=IgG1" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="IgG1 (56)" data-facet_lower="igg1" class="search_facet_name text-break isotype"> IgG1 <span class="facet_count">(56)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?isotype=IgG" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="IgG (273)" data-facet_lower="igg" class="search_facet_name text-break isotype"> IgG <span class="facet_count">(273)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?isotype=IgG2B" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="IgG2B (85)" data-facet_lower="igg2b" class="search_facet_name text-break isotype"> IgG2B <span class="facet_count">(85)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?isotype=IgG2 Alpha" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="IgG2 Alpha (29)" data-facet_lower="igg2 alpha" class="search_facet_name text-break isotype"> IgG2 Alpha <span class="facet_count">(29)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?isotype=IgG2b Kappa" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="IgG2b Kappa (1)" data-facet_lower="igg2b kappa" class="search_facet_name text-break isotype"> IgG2b Kappa <span class="facet_count">(1)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?isotype=Serum" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="Serum (1)" data-facet_lower="serum" class="search_facet_name text-break isotype"> Serum <span class="facet_count">(1)</span> </span> </a> </div> </div> </div> </div> <div class="search_facet_category_block"> <div class="dropdown-container position-relative"> <button class="btn btn-secondary dropdown-toggle more-facet" type="button" id="dropdown-menu-button-clone" data-toggle="dropdown" aria-haspopup="true" aria-expanded="false"> Clone </button> <div class="dropdown-menu" aria-labelledby="dropdown-menu-button-clone"> <div class="facets_wrapper"> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?clone=241812" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="241812 (28)" data-facet_lower="241812" class="search_facet_name text-break clone"> 241812 <span class="facet_count">(28)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?clone=H1alpha67" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="H1alpha67 (56)" data-facet_lower="h1alpha67" class="search_facet_name text-break clone"> H1alpha67 <span class="facet_count">(56)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?clone=2443B" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="2443B (31)" data-facet_lower="2443b" class="search_facet_name text-break clone"> 2443B <span class="facet_count">(31)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?clone=2443C" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="2443C (31)" data-facet_lower="2443c" class="search_facet_name text-break clone"> 2443C <span class="facet_count">(31)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?clone=ESEE122" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="ESEE122 (26)" data-facet_lower="esee122" class="search_facet_name text-break clone"> ESEE122 <span class="facet_count">(26)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?clone=241809" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="241809 (1)" data-facet_lower="241809" class="search_facet_name text-break clone"> 241809 <span class="facet_count">(1)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?clone=H1alpha 67-7" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="H1alpha 67-7 (29)" data-facet_lower="h1alpha 67-7" class="search_facet_name text-break clone"> H1alpha 67-7 <span class="facet_count">(29)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?clone=HA111" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="HA111 (29)" data-facet_lower="ha111" class="search_facet_name text-break clone"> HA111 <span class="facet_count">(29)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?clone=HIF1A/84" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="HIF1A/84 (1)" data-facet_lower="hif1a/84" class="search_facet_name text-break clone"> HIF1A/84 <span class="facet_count">(1)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?clone=JE75-33" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="JE75-33 (1)" data-facet_lower="je75-33" class="search_facet_name text-break clone"> JE75-33 <span class="facet_count">(1)</span> </span> </a> </div> </div> </div> </div> </div> <div class="search_facet_category_block"> <div class="dropdown-container position-relative"> <button class="btn btn-secondary dropdown-toggle more-facet" type="button" id="dropdown-menu-button-low_endotoxin" data-toggle="dropdown" aria-haspopup="true" aria-expanded="false"> Low Endotoxin </button> <div class="dropdown-menu" aria-labelledby="dropdown-menu-button-low_endotoxin"> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?low_endotoxin=False" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="False (16)" data-facet_lower="false" class="search_facet_name text-break low_endotoxin"> No <span class="facet_count">(16)</span> </span> </a> </div> </div> </div> </div> <div class="search_facet_category_block"> <div class="dropdown-container position-relative"> <button class="btn btn-secondary dropdown-toggle more-facet" type="button" id="dropdown-menu-button-has_sample_size" data-toggle="dropdown" aria-haspopup="true" aria-expanded="false"> Sample Size </button> <div class="dropdown-menu" aria-labelledby="dropdown-menu-button-has_sample_size"> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?has_sample_size=True" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="True (25)" data-facet_lower="true" class="search_facet_name text-break has_sample_size"> Yes <span class="facet_count">(25)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?has_sample_size=False" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="False (420)" data-facet_lower="false" class="search_facet_name text-break has_sample_size"> No <span class="facet_count">(420)</span> </span> </a> </div> </div> </div> </div> <div class="search_facet_category_block"> <div class="dropdown-container position-relative"> <button class="btn btn-secondary dropdown-toggle more-facet" type="button" id="dropdown-menu-button-stars" data-toggle="dropdown" aria-haspopup="true" aria-expanded="false"> Reviews </button> <div class="dropdown-menu" aria-labelledby="dropdown-menu-button-stars"> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?stars=5.0" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="5.0 (8)" data-facet_lower="5.0" class="search_facet_name text-break stars"> <span class="searchImage_star5"></span> 5 (8) </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?stars=4.0" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="4.0 (9)" data-facet_lower="4.0" class="search_facet_name text-break stars"> <span class="searchImage_star4"></span> 4 & up (17) </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?stars=3.0" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="3.0 (2)" data-facet_lower="3.0" class="search_facet_name text-break stars"> <span class="searchImage_star3"></span> 3 & up (19) </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?stars=1.0" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="1.0 (1)" data-facet_lower="1.0" class="search_facet_name text-break stars"> <span class="searchImage_star1"></span> 1 & up (20) </span> </a> </div> </div> </div> </div> <div class="search_facet_category_block"> <div class="dropdown-container position-relative"> <button class="btn btn-secondary dropdown-toggle more-facet" type="button" id="dropdown-menu-button-gene_symbol" data-toggle="dropdown" aria-haspopup="true" aria-expanded="false"> Gene Symbol </button> <div class="dropdown-menu" aria-labelledby="dropdown-menu-button-gene_symbol"> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?gene_symbol=HIF1A" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="HIF1A (445)" data-facet_lower="hif1a" class="search_facet_name text-break gene_symbol"> HIF1A <span class="facet_count">(445)</span> </span> </a> </div> </div> </div> </div> <div class="search_facet_category_block"> <div class="dropdown-container position-relative"> <button class="btn btn-secondary dropdown-toggle more-facet" type="button" id="dropdown-menu-button-brand" data-toggle="dropdown" aria-haspopup="true" aria-expanded="false"> Brand </button> <div class="dropdown-menu" aria-labelledby="dropdown-menu-button-brand"> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?brand=rnd" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="rnd (16)" data-facet_lower="rnd" class="search_facet_name text-break brand"> R&D Systems <span class="facet_count">(16)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/hif-1-alpha-hif1a/antibodies?brand=novus" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="novus (431)" data-facet_lower="novus" class="search_facet_name text-break brand"> Novus Biologicals <span class="facet_count">(431)</span> </span> </a> </div> </div> </div> </div> <div class="text-center mx-2 my-2"> <a href="#" id="show-more-less-facets" class="d-inline font-weight-bold h5">Show More</a> </div> </div> </div> </div> </div> <div class="row"> <div class="col-md-6 d-none d-lg-block"> <p> <span class="search_num_results" data-search_results="447">447 results</span> for "<span class="search_term_text">HIF-1 alpha/HIF1A Primary Antibodies</span>" in <strong id="search_category">Products</strong> </p> </div> <div class="col-md-6 text-lg-right"> <label for="sort_by_select">Sort by:</label> <select id="sort_by_select" class="sort_by_select rounded-0 p-0" aria-label="Sort by"> <option value="/t/hif-1-alpha-hif1a/antibodies?&sort=ranking,desc" selected="selected" >Best Match </option> <option value="/t/hif-1-alpha-hif1a/antibodies?&sort=title,asc" >Products (A-Z) </option> <option value="/t/hif-1-alpha-hif1a/antibodies?&sort=product_created_date,desc" >Newest </option> <option value="/t/hif-1-alpha-hif1a/antibodies?&sort=stars,desc" >Rating </option> </select> </div> </div> <div id="search_results" class="row mt-3" data-search_term="HIF-1 alpha/HIF1A Primary Antibodies"> <div class="col-lg-12"> <div class="d-none d-lg-block"> <p class="target_h1">HIF-1 alpha/HIF1A: Antibodies</p> <div class="show-more" data-text="<p>Bio-Techne offers high quality HIF-1 alpha/HIF1A antibodies from our Novus Biologicals and R&D Systems brands. Our HIF-1 alpha/HIF1A antibodies have been cited in 2491 publications with 63 5-star customer reviews. Browse our catalog for anti-HIF-1 alpha/HIF1A monoclonal, polyclonal, and recombinant monoclonal antibodies. These antibodies are reactive in target species including Human, Mouse, Rat, Canine, Bovine, and more. Our HIF-1 alpha/HIF1A antibodies have been validated for use in several research applications: Simple Western, Knockout Validated, Knockdown Validated, Dual RNAscope ISH-IHC, Immunohistochemistry, and more. Choose the anti-HIF-1 alpha/HIF1A antibody best suited for your research needs by viewing the technical details, images, citations, reviews, and more. Each HIF-1 alpha/HIF1A antibody is fully covered by Bio-Techne’s 100% guarantee. <br/><br/>Bio-Techne offers a comprehensive range of other <a href="https://www.bio-techne.com/t/hif-1-alpha-hif1a">HIF-1 alpha/HIF1A-related products</a> including <a href="https://www.bio-techne.com/t/hif-1-alpha-hif1a/elisa-kits">HIF-1 alpha/HIF1A ELISA Kits</a>, <a href="https://www.bio-techne.com/t/hif-1-alpha-hif1a/proteins-enzymes">HIF-1 alpha/HIF1A Proteins and Enzymes</a>, and more.</p>"> <div class="data-display"> <p>Bio-Techne offers high quality HIF-1 alpha/HIF1A antibodies from our Novus Biologicals and R&D Systems brands. Our HIF-1 alpha/HIF1A antibodies have been cited in 2491 publications with 63 5-star customer reviews. Browse our catalog for anti-HIF-1 alpha/HIF1A monoclonal, polyclonal, and recombinant monoclonal antibodies. These antibodies are reactive in target species including Human, Mouse, Rat, Canine, Bovine, and more. Our HIF-1 alpha/HIF1A antibodies have been validated for use in several research applications: Simple Western, Knockout Validated, Knockdown Validated, Dual RNAscope ISH-IHC, Immunohistochemistry, and more. Choose the anti-HIF-1 alpha/HIF1A antibody best suited for your research needs by viewing the technical details, images, citations, reviews, and more. Each HIF-1 alpha/HIF1A antibody is fully covered by Bio-Techne’s 100% guarantee. <br><br>Bio-Techne offers a comprehensive range of other <a href="https://www.bio-techne.com/t/hif-1-alpha-hif1a">HIF-1 alpha/HIF1A-related products</a> including <a href="https://www.bio-techne.com/t/hif-1-alpha-hif1a/elisa-kits">HIF-1 alpha/HIF1A ELISA Kits</a>, <a href="https://www.bio-techne.com/t/hif-1-alpha-hif1a/proteins-enzymes">HIF-1 alpha/HIF1A Proteins and Enzymes</a>, and more.</p> </div> <a href="#" class="show-more-btn">Show More</a> </div> </div> <div id="search_results_selected_facets" class="my-2"> <div id="search_results_selected_facet_legend" class="d-inline-block"> Filters: </div> <a class="search_results_selected_facet mx-1 py-1 px-2 clear_facet btn btn-default" href="/t/hif-1-alpha-hif1a/antibodies" rel="nofollow"> Primary Antibodies </a> <a href="/t/hif-1-alpha-hif1a/antibodies" id="clear_filters">Clear All <strong>X</strong></a> </div> <div id="search_results_block"> <div class="search_result atc-container pb-3" data-brand="novus" data-category="Primary Antibodies" data-id="NB100-105" data-name="HIF-1 alpha Antibody (H1alpha67)" data-position="0"> <div class="search_details border bg-white p-3 rounded"> <div class="row"> <div class="col-md-9"> <a href="/p/antibodies/hif-1-alpha-antibody-h1alpha67_nb100-105" title="HIF-1 alpha Antibody (H1alpha67)"> <h4>HIF-1 alpha Antibody (H1alpha67) </h4> </a> </div> <div class="search_result_brand_logo col-md-3 mb-1 d-flex justify-content-xl-end product-brand-logo"> <img alt="Novus Biologicals, a Bio-Techne brand" src="https://resources.bio-techne.com/bio-techne-assets/images/logos/bio-techne-novus-biologicals.svg" class="d-flex w-75"> </div> </div> <div class="row"> <div class="col-md-9"> <div class="mb-1 d-inline-flex"> <div class="align-middle my-1 mr-3 d-block d-md-inline"> Catalog #: <a href="/p/antibodies/hif-1-alpha-antibody-h1alpha67_nb100-105" title="HIF-1 alpha Antibody (H1alpha67)"> NB100-105 </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold"> <a href="/p/antibodies/hif-1-alpha-antibody-h1alpha67_nb100-105#reviews" class="searchImage_star4"> (44) </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold"> <a href="/p/antibodies/hif-1-alpha-antibody-h1alpha67_nb100-105#citations"><span class="icon-icon-product-citations"></span>Citations (1052)</a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold text-nowrap"> <a href="/p/antibodies/hif-1-alpha-antibody-h1alpha67_nb100-105#product-documents"><span class="icon-icon-pdf text-error-red"></span> Product Documents</a> </div> <div class="d-none d-md-inline my-1"> <a href="#" class="search_result_images"><span class="icon-icon-image-camera-plus"></span> Images (116)</a> </div> </div> <div class="d-none d-md-block"> <span class="search_result_applications"></span><span class="applications_title"><strong>Applications:</strong></span> IHC, WB, ELISA, ICC/IF, Flow, B/N, ChIP, Func, GS, IP, KD, KO, NULL, PAGE, Simple Western </div> <div class="d-none d-md-block"> <span class="species_title"><strong>Reactivity:</strong></span> Human, Mouse, Rat, Canine, Monkey, +10 More </div> </div> <div class="col-md-3"> <button class="py-1 btn-default btn-block atc-view-more collapsed" data-toggle="collapse" href="#collapseButton-0" role="button" aria-expanded="false" aria-controls="collapseButton">View More</button> </div> </div> <div class="commerce-atc-search pt-3 collapse" id="collapseButton-0"> <div class="row"> <div class="search_result_description col-xs-12 col-md-9"> <table class="tablesaw tablesaw-stack mb-2"> <tbody> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Reactivity:</strong></td> <td class="pb-1 w-75 text-break"> Human, Mouse, Rat, Canine, Monkey, +10 More </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Details:</strong></td> <td class="pb-1 w-75 text-break"> Mouse IgG2B Monoclonal Clone #H1alpha67 </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Applications:</strong></td> <td class="pb-1 w-75 text-break"> IHC, WB, ELISA, ICC/IF, Flow, +10 More </td> </tr> </tbody> </table> <div class="compare_tool_select form-inline mb-2" data-sku="nb100-105"></div> </div> <div class="search_result_image_wrapper col-xs-12 col-md-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="novus"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src=https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-H1alpha67-Western-Blot-NB100-105-img0007.jpg data-srcset=https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-H1alpha67-Western-Blot-NB100-105-img0007.jpg alt="Western Blot Analysis of HIF-1 alpha in Caki-1 Cell Lysates" title="Western Blot Analysis of HIF-1 alpha in Caki-1 Cell Lysates" data-caption="HIF-1 alpha induction by CoCl2 on Caki-1 cell lysate. WB image submitted by a verified customer review." data-badges="<span class="biological-validation"></span>" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> <span class="image-count-text align-middle">(116)</span> </div> </div> </div> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-H1alpha67-Knockout-Validated-NB100-105-img0027.jpg" data-alt="Knockout Validation of HIF-1 alpha Antibody in HeLa and HIF-1 Knockout HeLa Cells" title="Knockout Validation of HIF-1 alpha Antibody in HeLa and HIF-1 Knockout HeLa Cells" data-caption="HIF-1 alpha was detected in immersion fixed DFO treated Hela cells (left) but was not detected in HIF-1 knockout HeLa cells (right) using Mouse Anti-human HIF-1 alpha monoclonal antibody (Catalog # NB100-105) at 25 ug/mL for 3 hours at room temperature. Cells were stained using a NorthernLights (TM) 557-conjugated Donkey Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei." data-badges="<span class="knockout-validation"></span>" data-application="Knockout Validated" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-H1alpha67-Western-Blot-NB100-105-img0026.jpg" data-alt="Detection of HIF-1 alpha in WT and HIF-1 alpha Knockout Mouse Cells by Western Blot" title="Detection of HIF-1 alpha in WT and HIF-1 alpha Knockout Mouse Cells by Western Blot" data-caption="Naive CD4 T cells from WT, VHL-deficient (Vhl KO), or HIF-1 alpha-deficient (HIF-1 alpha KO) mice were differentiated under IL-22-skewing conditions for a total of 60 h. Some cells remained at normoxia for the duration of the culture (N); others were at normoxia for 35 h and then hypoxia (1% O2) for 24 h (H). At 60 h, nuclear extracts were harvested, and HIF-1 alpha and Lamin B1 levels were analyzed by Western blot. Image from verified customer review." data-badges="<span class="knockout-validation"></span><span class="biological-validation"></span>" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-H1alpha67-Flow-Cytometry-NB100-105-img0017.jpg" data-alt="Flow Cytometry of Myeloma Cells Stained with Alexa Fluor 488 Conjugated HIF-1 alpha Antibody" title="Flow Cytometry of Myeloma Cells Stained with Alexa Fluor 488 Conjugated HIF-1 alpha Antibody" data-caption="Analysis using the Alexa Fluor (R) 488 conjugate of NB100-105. Staining of HIF-1 alpha in multiple myeloma cells: H929 cells (0.5 x 10^6) were stained with Alexa Fluor 488(R) conjugated HIF-1 alpha antibody (NB100-105AF488). Image courtesy of Dr. Barbara Muz at Washington University in St. Louis School of Medicine." data-badges="" data-application="Flow Cytometry" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-H1alpha67-Immunohistochemistry-NB100-105-img0004.jpg" data-alt="Immunohistochemical Staining of HIF-1 alpha in Human Kidney" title="Immunohistochemical Staining of HIF-1 alpha in Human Kidney" data-caption="Staining of HIF-1 alpha in human kidney using NB100-105. Renal tubular epithelium showed moderate membranous, cytoplasmic and nuclear staining, and glomeruli showed faint to moderate nuclear staining." data-badges="" data-application="Immunohistochemistry" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-H1alpha67-Western-Blot-NB100-105-img0029.jpg" data-alt="Western Blot Analysis of HIF-1 alpha in Nuclear-Enriched and Whole Cell Extracts from Non-Diabetic and T2D Donor Islets" title="Western Blot Analysis of HIF-1 alpha in Nuclear-Enriched and Whole Cell Extracts from Non-Diabetic and T2D Donor Islets" data-caption="HIF1alpha/PFKFB3 is upregulated in beta-cells of HIP rats and humans with type 2 diabetes. Representative Western blot of PFKFB3 and HIF1alpha levels in nuclear-enriched- and whole cell extracts from non-diabetic (ND) and T2D donor islets. Data are presented as mean +/- SEM, n = 3 independent biological samples for each group. Statistical significance was analyzed by Student t-test (*p < 0.05, ***p < 0.001) Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41467-019-10444-1) licensed under a CC-BY license." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-H1alpha67-Immunohistochemistry-Paraffin-NB100-105-img0020.jpg" data-alt="Immunohistological Staining of HIF-1 alpha in Paraffin Embedded Human Glioblastoma Multiforme" title="Immunohistological Staining of HIF-1 alpha in Paraffin Embedded Human Glioblastoma Multiforme" data-caption="Analysis using the biotin conjugate of NB100-105. Staining of human glioblastoma multiforme." data-badges="" data-application="Immunohistochemistry-Paraffin" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-H1alpha67-Immunocytochemistry-Immunofluorescence-NB100-105-img0023.jpg" data-alt="Staining of HIF-1 alpha in DFOA Treated and non-Treated HeLa Cells" title="Staining of HIF-1 alpha in DFOA Treated and non-Treated HeLa Cells" data-caption="HIF-1 alpha was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line treated with DFOA using 1 ug/mL of mouse anti-HIF-1 alpha monoclonal antibody NB100-105. Cells were stained using a donkey anti-rabbit secondary antibody and counterstained with DAPI (blue)." data-badges="<span class="biological-validation"></span>" data-application="Immunocytochemistry/ Immunofluorescence" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-H1alpha67-Western-Blot-NB100-105-img0032.jpg" data-alt="Detection of HIF-1 alpha by Western Blot in Multiple Cells Lines with Various Treatments" title="Detection of HIF-1 alpha by Western Blot in Multiple Cells Lines with Various Treatments" data-caption="Upregulation of HIF-1alpha in human PCa. Protein expression of HIF-1alpha, VEGF, and GLUT4 were examined with western blot, in PC-3, DU145, and LNCaP cells after various treatments as indicated. Data are expressed as mean +/- SD of seven independent experiments. $p < 0.05 versus RWPE-1 or BPH1 cells or normal tissue. *p < 0.05 versus control group. #p < 0.05 versus si-HIF-1alpha or DDP group. Original blots are shown in Supplementary Figure 5. C: Ctrl; D: DDP; S: si-HIF-1alpha; D/S: DDP/si-HIF-1alpha. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41598-017-07973-4), licensed under a CC-BY license." data-badges="<span class="biological-validation"></span>" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-H1alpha67-Immunohistochemistry-Paraffin-NB100-105-img0034.jpg" data-alt="Staining of HIF-1 alpha in Paraffin Embedded Human Throat Cancer" title="Staining of HIF-1 alpha in Paraffin Embedded Human Throat Cancer" data-caption="Analysis using HIF-1 alpha Antibody on human throat cancer tissue. PFA fixed paraffin-embedded sections. Primary antibody dilution 1:100. Image from verified customer review." data-badges="" data-application="Immunohistochemistry-Paraffin" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-H1alpha67-Western-Blot-NB100-105-img0021.jpg" data-alt="Western Blot Analysis of HIF-1 alpha in Human Hepatocytes" title="Western Blot Analysis of HIF-1 alpha in Human Hepatocytes" data-caption="Analysis of HIF-1 alpha in human hepatocytes from cancer patient using anti-HIF-1 alpha antibody NB100-105. Western blot image submitted by a verified customer review." data-badges="<span class="biological-validation"></span>" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-H1alpha67-Western-Blot-NB100-105-img0022.jpg" data-alt="Use of HRP Conjugated HIF-1 alpha Antibody in Western Blotting of COS-7 Nuclear Extracts" title="Use of HRP Conjugated HIF-1 alpha Antibody in Western Blotting of COS-7 Nuclear Extracts" data-caption="Analysis using the HRP conjugate of NB100-105. Detection of 50 ug cobalt chloride induced COS-7 nuclear extracts (NB800-PC26) using NB100-105." data-badges="<span class="biological-validation"></span>" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-H1alpha67-Western-Blot-NB100-105-img0030.jpg" data-alt="Western Blot of HIF-1 alpha in CD11c+ Cells from C57BL/6 Mice and Densitometric Analysis" title="Western Blot of HIF-1 alpha in CD11c+ Cells from C57BL/6 Mice and Densitometric Analysis" data-caption="L. donovani infection induces HIF-1 alpha expression in CD11chi splenic DCs in an IRF-5 dependent manner. Mice were infected with 2 x 10^7 amastigotes intravenously. Immunoblot analysis of HIF-1 alpha expression in CD11c+ cells from C57BL/6 mice (upper panel) and densitometric analysis normalized to beta-actin expression and expressed as fold increase to results obtained with naive mice (lower panel). Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.ppat.1004938), licensed under a CC-BY license." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-H1alpha67-Western-Blot-NB100-105-img0031.jpg" data-alt="Western Blot Detection of HIF-1 alpha in Nonmalignant and PCa Cell Lines" title="Western Blot Detection of HIF-1 alpha in Nonmalignant and PCa Cell Lines" data-caption="Upregulation of HIF-1alpha in human Pca. HIF-1alpha protein was detected by western blot in nonmalignant (RWPE-1 and BPH1) and PCa cell lines (PC-3, DU145, LNCaP, and 22RV1) as indicated. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41598-017-07973-4), licensed under a CC-BY license." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-H1alpha67-Immunohistochemistry-NB100-105-img0033.jpg" data-alt="Immunohistological Detection of HIF-1 alpha in PDAC and Adjacent Normal Pancreatic Tissue" title="Immunohistological Detection of HIF-1 alpha in PDAC and Adjacent Normal Pancreatic Tissue" data-caption="HIF-1alpha expression in PDAC and adjacent normal pancreatic tissue. PDAC with weak (intensity 1) nuclear and cytoplasmic HIF-1alpha staining (left). PDAC with strong (intensity 3) nuclear and moderate (intensity 2) cytoplasmic HIF-1alpha staining (right). Image collected and cropped by CiteAb from the following publication (https://wjso.biomedcentral.com/articles/10.1186/s12957-018-1432-4), licensed under a CC-BY license." data-badges="" data-application="Immunohistochemistry" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-H1alpha67-Flow-Cytometry-NB100-105-img0016.jpg" data-alt="Flow Cytometry of H929 Cells Stained with Alexa Fluor 488 Conjugated HIF-1 alpha Antibody" title="Flow Cytometry of H929 Cells Stained with Alexa Fluor 488 Conjugated HIF-1 alpha Antibody" data-caption="Analysis using the Alexa Fluor(R) 488 conjugate of NB100-105. Staining of HIF-1 alpha in H929 cells using HIF-1 alpha antibody. Flow cytometry image submitted by a verified customer review." data-badges="" data-application="Flow Cytometry" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-271220231261949.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Effects of one- and five-dosage dry needling (1D, 5D) on iNOS, HIF-1 alpha, COX-2, and VEGF expressions in a needling-treated muscle. (a) Representative Western blot images. The quantification of the protein levels for (b) iNOS, (c) HIF-1 alpha, (d) COX-2, and (e) VEGF. Values are expressed as mean ± SD. *Indicates the significant difference (P < 0.05) between the sham groups (s1D and s5D). #Represents the significant difference (P < 0.05) between the 1D and 5D groups. DN: dry needling." data-badges="<span class="biological-validation"></span>" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-271220231261920.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Hypoxia is implicated in the adaptive response after short-term bevacizumab treatment. Expression of HIF-1 alpha in pulmonary tumor nodules of the four groups. (A) A representative western blot is shown. beta-actin was used as a loading control. (B) While most tumors showed little expression of HIF-1 alpha protein in PBS and cisplatin groups, mice that received bevacizumab and bevacizumab + cisplatin therapy showed a markedly increased level of HIF-1 alpha expression.. *P < 0.05, **P < 0.01." data-badges="<span class="biological-validation"></span>" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-271220231253311.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Effects of F2 on tumor-related biomarkers in vivoImmunohistochemical analysis of tumor-related proteins (HIF-1 alpha, VEGF, MMP-2, CD31 and KI67) in tumor sections from U87 xenograft mice treated with F2 (mg/kg i.g.). Red arrows indicate the related protein detected in the tissue samples. Values are means ± SEM of three experiments. * P" data-badges="<span class="biological-validation"></span>" data-application="Immunohistochemistry-Paraffin" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-271220231261914.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="AAM inhibits hypoxia-induced VM formation involving HIF-1 alpha and MMP2. The HCT-116 (A) and LoVo (B) cells were treated with different concentrations of AAM under hypoxia and normoxia; the protein expression of HIF-1 alpha and MMP2 was detected by western blotting. (C, D) The mRNA expression of HIF-1 alpha and MMP2 was measured by RT-PCR (*P < 0.05, **P < 0.01, ***P < 0.001)." data-badges="<span class="biological-validation"></span>" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-271220231285614.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Immunohistochemical analysis of STC1 expression in esophageal cancer. (A) Normal squamous epithelium was negative for STC1 expression. (B) Normal gastric glands showed positive staining for STC1, which was used as a positive control. (C) Positive staining for STC1 in esophageal squamous cell carcinoma (ESCC). (D) Negative staining for STC1 in ESCC. (E) Positive staining for HIF-1 alpha in ESCC. (F) Positive staining for p53 in ESCC. Magnification, ×100." data-badges="" data-application="Immunohistochemistry" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-30820241044555.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Simple Western: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Simple Western: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Effect of hypoxia on human airway epithelial cells (A549).A549 cells were subjected to hypoxia for 6, 24, and 48 hours. Cell lysates were collected and analyzed for the expression levels of various hypoxic markers by qPCR (n = 4). (A-C) The expression levels of HIF-1 alpha (A), GLUT-1 (B), and VEGFA (C) were measured. (D-G) Additionally, whole-lung lysate was used for Western blots to determine the protein expression of HIF-1 alpha, VEGFA, and FIH-1. After analyzing the data, we used the unpaired t test with Welch's correction to determine its statistical significance. *P < 0.05, ***P < 0.001, ****P < 0.0001. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/37737265 ), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Simple Western" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-21020242345240.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="The HIF-1 alpha-BNIP3 pathway was activated during hypoxia. (a) Analysis of mitophagy-related protein expression in Neuro-2a cells by immunoblotting. Neuro-2a cells were exposed to hypoxia and/or high glucose for 48 h. Total cellular extracts were analysed by immunoblotting with antibodies against HIF-1 alpha, BNIP3, PINK1 & TOMM20. (b) The ImageJ densitometric analysis is shown. “C” represented control conditions (25 mM glucose), “G” represented high glucose conditions (75 mM glucose). The results are expressed as the mean ± SEM from three independent experiments. Two-way ANOVA, **p " data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-310202415284537.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Inhibition of tumor growth in vivo by various treatments. (a) Macroscopic view of mouse tumors at the end of the study. (b) Tumor growth curves from day 0 to 40, with tumor sizes measured every 5 days, in various treatment groups as indicated. (c) Wet weight of tumors measured at the end of the study. (d) Protein expression of HIF-1 alpha examined by western blot in PC-3 xenografts exposed to various treatments. Data were presented as mean ± SD (n = 6). *p " data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-310202415371973.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - AAM inhibits hypoxia-induced VM formation involving HIF-1 alpha & MMP2. The HCT-116 (A) & LoVo (B) cells were treated with different concentrations of AAM under hypoxia & normoxia; the protein expression of HIF-1 alpha & MMP2 was detected by western blotting. (C, D) The mRNA expression of HIF-1 alpha & MMP2 was measured by RT-PCR (*P < 0.05, **P < 0.01, ***P < 0.001). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32499699), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-310202415371945.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Muscle repair following eccentric contraction–induced injury is concomitant with dynamic alterations of HIF2A & HIF1A expression in SCs.(A–C) Representative images of EDL myofibers from injured muscles at various time points (n >50 myofibers from 3 mice/group/time point) & stained for Pax7, DAPI, & EdU (A), HIF2A (B), or HIF1A (C). Scale bars: 20 μm. Arrowheads indicate SCs. (D) Number of Pax7+ SCs per myofiber at various time points. (E) Percentage of EdU+ SCs at various time points. (F) Percentage of HIF2A+ SCs at various time points. (G) Percentage of HIF1A+ SCs at various time points. Data represent the mean ± SEM. Image collected & cropped by CiteAb from the following publication (https://www.jci.org/articles/view/96208), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Immunocytochemistry/ Immunofluorescence" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-310202415371960.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - mTORC2 not mTORC1 regulates HIF‐2 alpha, GOT1 in PDAC under prolonged hypoxia. (A & B) HIF‐2 alpha mRNA & protein levels determined by qRT‐PCR (mRNA) & Western blot (protein) after treatment of Panc‐1 & Capan‐2 cells with mTORC1 inhibitor rapamycin for 48 hrs at 3% or 1% O2. beta‐Actin was used as loading control. (C & D) Panc‐1 & Capan‐2 cells were treated with mTORC1/mTORC2 inhibitor PP242 & cultured for 48 hrs at 20%, 3% or 1% O2, & HIF‐2 alpha, GOT1 mRNA & protein levels were determined by qRT‐PCR (mRNA) & Western blot (protein). beta‐Actin was used as loading control. Data are presented as mean ± S.D. from three independent experiments. *P < 0.05, **P < 0.01. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28544376), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-31020241539593.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - mTORC2 not mTORC1 regulates HIF‐2 alpha, GOT1 in PDAC under prolonged hypoxia. (A & B) HIF‐2 alpha mRNA & protein levels determined by qRT‐PCR (mRNA) & Western blot (protein) after treatment of Panc‐1 & Capan‐2 cells with mTORC1 inhibitor rapamycin for 48 hrs at 3% or 1% O2. beta‐Actin was used as loading control. (C & D) Panc‐1 & Capan‐2 cells were treated with mTORC1/mTORC2 inhibitor PP242 & cultured for 48 hrs at 20%, 3% or 1% O2, & HIF‐2 alpha, GOT1 mRNA & protein levels were determined by qRT‐PCR (mRNA) & Western blot (protein). beta‐Actin was used as loading control. Data are presented as mean ± S.D. from three independent experiments. *P < 0.05, **P < 0.01. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28544376), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-310202415371957.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Murine tumor cells express connexin 43 (Cx43) & hypoxic-induced factor-1 alpha (HIF-1 alpha). The expression of Cx43 & HIF-1 alpha was measured by Western blot analysis. beta-actin expression served as loading controls & total protein. Inserted values indicated relative protein expression in comparison with beta-actin. Image collected & cropped by CiteAb from the following publication (http://www.mdpi.com/1422-0067/16/1/439), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-310202415395927.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Representative microscopy images of staining for hypoxia markers in prostate tissues (MO, 400×). A) HIF-1 alpha - notice the granular cytoplasmic immunoreactivity of the malignant epithelial cells. In this case, more than 50% of the glands stained. B) LOX - strong & diffuse nuclear immunoreactivity of the epithelial cells. C) CAIX - note a focal apical cytoplasmic immunoreactivity in epithelial cells. D) VEGFR2 - moderate nuclear & weak cytoplasmic expression of the epithelial cells Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28143503), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Immunohistochemistry" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-310202415345343.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Effect of renal medullary transfection of PHD2 plasmids on the levels of PHD2 & HIF-1 alpha in the renal medulla. (A) PHD2 mRNA levels. (B) Representative ECL gel documents of Western blot analyses depicting the protein levels of HIF-1 alpha. (C) Summarized intensities of the HIF-1 alpha blots (normalized to LS). *P < 0.05 versus others (n = 7–8). LS = low salt, HS = high salt, Ctrl = control vectors, PHD2 = PHD2 expression vectors, CoCl2 = sample from cells treated with CoCl2 as positive control. Renal medullary tissues were obtained at the end of blood pressure recording after 3 week high salt challenge. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/22686466), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-310202415334940.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Immunohistochemical analysis of STC1 expression in esophageal cancer. (A) Normal squamous epithelium was negative for STC1 expression. (B) Normal gastric glands showed positive staining for STC1, which was used as a positive control. (C) Positive staining for STC1 in esophageal squamous cell carcinoma (ESCC). (D) Negative staining for STC1 in ESCC. (E) Positive staining for HIF-1 alpha in ESCC. (F) Positive staining for p53 in ESCC. Magnification, ×100. Image collected & cropped by CiteAb from the following publication (https://www.spandidos-publications.com/10.3892/or.2011.1607), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Immunohistochemistry" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-31020241535668.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - HIF-1alpha & CAIX expression in PDAC & adjacent normal pancreatic tissue. Adjacent normal exocrine pancreas with weak nuclear & cytoplasmic HIF-1alpha staining (a). Adjacent normal pancreatic tissue with weak membranous CAIX staining in the ducts & absent staining in exocrine pancreatic tissue (b). PDAC with weak (intensity 1) nuclear & cytoplasmic HIF-1alpha staining (c). PDAC with strong (intensity 3) nuclear & moderate (intensity 2) cytoplasmic HIF-1alpha staining (d). PDAC with weak to moderate (intensity 1–2) membranous CAIX staining (e). PDAC with strong (intensity 3) membranous CAIX staining (f) Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29973215), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Immunohistochemistry-Paraffin" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-31020241535620.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - HIF-1 alpha staining within injured muscle area (representative figure).The animal was ventilated with 100% O2. 450 minutes after trauma, the Musculus gastrocnemius of the traumatized right hind limb was harvested & section of the muscle specimen was analyzed for HIF-1 alpha expression within the injured area. No significant staining for HIF-1 alpha of the muscle cells. Scale bar: 150 µm. Inlet: Positive staining for HIF-1 alpha expression by myeloid cells (brown), invading to the traumatized muscle. Scale bar: 10 µm. Image collected & cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0111151), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Immunohistochemistry" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-31020241534367.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - HIF1 alpha is activated in VSMCs during Ang II-induced vascular remodeling.WT mice were infused with saline or 1000 ng/kg/min Ang II for 28 days. a Immunofluorescence analysis of representative cross-sections of mice aortas for HIF1 alpha (red) & alpha-SMA (green), nuclei was stained with DAPI. VSMCs were isolated form WT mice & treated with 1 μM Ang II for 24 h. bHif1a mRNA was measured by qPCR analysis. c HIF1 alpha protein was detected by western blot. **P " data-badges="" data-application="Immunocytochemistry/ Immunofluorescence" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-310202415171348.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - HIF-1 alpha pathway activation is crucial for CD24+ NP cells maintenance. a-b Western blot analysis of HIF-1 alpha expression in CD24+, CD24− & unsorted NP cells. c Immunofluorescence staining analysis of CD24 in WT & NP-specific HIF-1 alpha knock out mice, scale bars represent 100 μm. d-e Fluorescence activated cell sorting analysis of the percentage of CD24+ NP cells after Hif-1 alpha or Vhl knock down. f Immunofluorescence staining analysis of CD24 in NP cells after Hif-1 alpha or Vhl knock down, scale bars represent 25 μm. *P " data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-310202415392591.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - HIF‐2 alpha depletion counteracts tumour growth & non‐canonical glutamine metabolism in vivo. (A) Representative images of tumour‐bearing mice & tumours removed from the mice. (B) Tumour volumes were measured on the indicated days. (C) Tumour weights were shown as the means ± S.D. when the tumours were harvested. (D) Representative images (×200) of H&E & IHC staining of the tumours. Ki67 was used as proliferation index. (E & F) qRT‐PCR & Western blot analysed the expression of HIF‐2 alpha & GOT1 in tumour tissues from sh‐HIF‐2 alpha Panc‐1 group compared with control group, *P < 0.05, **P < 0.01. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28544376), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-310202415395944.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Hypoxia is implicated in the adaptive response after short-term bevacizumab treatment. Expression of HIF-1 alpha in pulmonary tumor nodules of the four groups. (A) A representative western blot is shown. beta-actin was used as a loading control. (B) While most tumors showed little expression of HIF-1 alpha protein in PBS & cisplatin groups, mice that received bevacizumab & bevacizumab + cisplatin therapy showed a markedly increased level of HIF-1 alpha expression.. *P < 0.05, **P < 0.01. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/22357313), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-310202415395930.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - HIF-1alpha & CAIX expression in PDAC & adjacent normal pancreatic tissue. Adjacent normal exocrine pancreas with weak nuclear & cytoplasmic HIF-1alpha staining (a). Adjacent normal pancreatic tissue with weak membranous CAIX staining in the ducts & absent staining in exocrine pancreatic tissue (b). PDAC with weak (intensity 1) nuclear & cytoplasmic HIF-1alpha staining (c). PDAC with strong (intensity 3) nuclear & moderate (intensity 2) cytoplasmic HIF-1alpha staining (d). PDAC with weak to moderate (intensity 1–2) membranous CAIX staining (e). PDAC with strong (intensity 3) membranous CAIX staining (f) Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29973215), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Immunohistochemistry-Paraffin" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-31020241535697.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - DC101, evofosfamide, & low dose doxorubicin multimodal treatment of KP sarcomasA. Tumor volume of KP sarcomas. Groups were treated by intraperitoneal injection of control IgG 20 mg/kg 3 times per week, DC101 20 mg/kg 3 times per week, evofosfamide (Evo) 50 mg/kg 5 times per week, and/or low dose doxorubicin (Dox) 1 mg/kg 3 times per week. Photos B. & graphs C. of PCNA immunohistochemistry for proliferation, TUNEL immunofluorescence for apoptosis, CD31 immunohistochemistry for microvessel density, CD31 & TUNEL immunofluorescence for endothelial cell (EC)-specific apoptosis, & nuclear HIF-1 alpha immunohistochemistry for HIF-1 alpha activity. Bars represent standard deviation. **p < 0.05 compared to all other groups. Image collected & cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.10212), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Immunohistochemistry" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-31020241538246.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Overexpression of HIF alpha proteins increases IL-1 alpha transcription in A549 & HeLa cells but not in WI-38 fibroblasts.(A) Western blot using anti-HIF-1 alpha or anti-HIF-2 alpha antibodies of nuclear fractions obtained from HEK-T293 cells transfected with the annotated vectors. WI-38 (B), A549 (C), or HeLa (D) cells were transfected with either control vector or with HIF-1 alpha (P402A/P564A) & HIF-2 alpha (P405A/P531A), & 24 h later were analyzed for IL-1 alpha & VEGF RQ of mRNA levels by real-time PCR. Graphs represent mean ± SEM of four independent experiments for each cell type. P values of HIF alpha-transfected cells vs. control (Ct) transfected are annotated in the graphs. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/23049530), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-310202415382424.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Effects of F2 on tumor-related biomarkers in vivoImmunohistochemical analysis of tumor-related proteins (HIF-1 alpha, VEGF, MMP-2, CD31 & KI67) in tumor sections from U87 xenograft mice treated with F2 (mg/kg i.g.). Red arrows indicate the related protein detected in the tissue samples. Values are means ± SEM of three experiments. * P" data-badges="" data-application="Immunohistochemistry-Paraffin" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-310202415284524.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Immunohistochemical labeling of normal brain & glioma tissueRepresentative images of normal temporal lobe, anaplastic astrocytoma grade II & grade III (AAII & AAIII), & three GBMs with low, medium, & high labeling of (A) MAOB, (B) HiF-1 alpha, & (C) GFAP are shown, along with (D) Sp1 & e) Sp3. The antibodies were detected with DAB & the nuclei were labeled with hematoxylin. Each image in a column was taken from a single slide, where each tissue slice was treated identically & imaged using the same microscope setting & with no electronic image manipulation. Arrows indicate nuclear HiF-1 alpha. Image collected & cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.6582), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Immunohistochemistry" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-31020241529190.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Expression of PACAP, VIP, VIPRs, HIF-1 alpha, HIF-2 alpha, & EGFR in glioblastoma multiforme (GBM). (A) Representative immunoblot of PACAP & VIP precursor peptides & PAC1R, VPAC1R, & VPAC2R expression on frozen glioblastoma sample. (B) Representative immunoblot & photomicrographs of signals detected by antibodies direct against HIF-1 alpha, HIF-2 alpha, & EGFR in a frozen glioblastoma sample. Image collected & cropped by CiteAb from the following publication (http://journal.frontiersin.org/Article/10.3389/fphar.2016.00139/abstract), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-310202415395911.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Regulation of cartilage degradation by HIF-1 alpha. (A) Amount of glycosaminoglycans released into the medium determined by the dimethylmethylene blue assay during 3 days culture of Hif1afl/fl (Cntl) & Col2a1-CreERT2;Hif1afl/fl (KO) femoral heads treated with or without 10 ng/mL IL-1 beta under the hypoxic condition (3% O2). Tamoxifen induction was performed three days before sacrifice. Bars show the mean ± SD of three samples per group. *P " data-badges="" data-application="Immunocytochemistry/ Immunofluorescence" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-310202415291982.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - HIF1 alpha/PFKFB3 is upregulated in beta-cells of HIP rats & humans with type 2 diabetes. Representative immunofluorescence images of islets from a WT & HIP rats & b non-diabetic (ND) & T2D patients stained for PFKFB3 (red), insulin (green), & nuclei (blue). c Frequency of PFKFB3 positive beta-cells in HIP versus WT rats (left panel) & T2D versus ND patients (right panel). d Representative Western blot of PFKFB3 & HIF1 alpha levels in nuclear-enriched- & whole cell extracts from non-diabetic (ND) & T2D donor islets. Data are presented as mean ± SEM, n = 3 independent biological samples for each group. Statistical significance was analyzed by Student t-test (*p " data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-31020241517526.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - HIF-1 alpha & HIF-2 alpha expression is differentially modulated by Hsp90 inhibition in CCRCC cells. UMRC2 & 786-O cells were treated for 20 h with the Hsp90 inhibitors 17-AAG (1 μM), EC154 (100 nM), the HDAC inhibitor LBH589 (100 nM), or DMSO vehicle control. Nuclear protein was analyzed for HIF-1 alpha & HIF-2 alpha & cytosolic protein was analyzed for RACK1 by SDS-PAGE & Western blot. Topoisomerase II & GAPDH were used as nuclear & cytosolic loading controls, respectively. Bands corresponding to HIF-1 alpha & HIF-2 alpha Western blots were quantified, normalized to TOPOII, & are expressed as a percentage of control samples. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/22172030), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-310202415392560.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Comparison of MAOB expression, HiF-1 alpha expression, & peroxide generation in response to a MAOB substrate in normal human astrocytes & glioma cellsHiF-1 alpha (i, red), MAOB (ii, green), & superimposed HiF-1 alpha/MAOB signals with blue DAPI labeling (iii) of NHAs (A) & GBM cells (B). (C) Relative expression of HiF-1 alpha (white bars) & MAOB (black bars) in NHAs & in three GBM low-passage cultures, n = 6, mean ± SD. (D) MAOB inhibited (gray bars), endogenous (white bars) & 4-FBA-stimulated (black bars) reactive oxygen species (hydrogen peroxide) generation, shown as DCF per cell, in NHA & the three GBM cultures, n = 16, mean ± SD. Image collected & cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.6582), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Immunocytochemistry/ Immunofluorescence" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-310202415304218.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - HIF1 alpha is activated in VSMCs during Ang II-induced vascular remodeling.WT mice were infused with saline or 1000 ng/kg/min Ang II for 28 days. a Immunofluorescence analysis of representative cross-sections of mice aortas for HIF1 alpha (red) & alpha-SMA (green), nuclei was stained with DAPI. VSMCs were isolated form WT mice & treated with 1 μM Ang II for 24 h. bHif1a mRNA was measured by qPCR analysis. c HIF1 alpha protein was detected by western blot. **P " data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-310202415175262.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Effects of one- & five-dosage dry needling (1D, 5D) on iNOS, HIF-1 alpha, COX-2, & VEGF expressions in a needling-treated muscle. (a) Representative Western blot images. The quantification of the protein levels for (b) iNOS, (c) HIF-1 alpha, (d) COX-2, & (e) VEGF. Values are expressed as mean ± SD. *Indicates the significant difference (P < 0.05) between the sham groups (s1D & s5D). #Represents the significant difference (P < 0.05) between the 1D & 5D groups. DN: dry needling. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/23346198), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-310202415345342.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Effect of ENMD-1198 on tumor vascularization & tumor cell proliferation. A) Immunohistochemical analysis of tumor vascularization (CD31-positive vessels). Densitometric analysis of images from CD31-stained tissue sections from all tumors showed that vessel area was significantly reduced in ENMD-1198-treated tumors (*P < 0.01). B) ENMD-1198 significantly decreased the number of proliferating (BrdUrd-positive) tumor cells in tissue sections (*P < 0.01). For both vessel area & cell proliferation, representative images are illustrated. Results shown are the mean ± SEM. C) Western blot analysis for HIF-1 alpha expression in HCC tumors showed a substantial reduction of HIF-1 alpha in ENMD-1198 treated tumors. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/18651980), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-310202415175253.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Enhanced expression of glycolytic genes in livers of DEN-Wwox deltaHep mice.a mRNA expression levels of HIF1 alpha glycolytic target genes in DEN-treated control & Wwox deltaHep (cKO) mice at the age of 1 a, 3 b, 6 c & 10 d months (n = 3 for each group). e Immunohistochemical staining of HIF1 alpha, PKM2, & GLUT1 in 6 & 10 months of DEN-treated Wwox deltaHep & control liver tissues. Images were taken at × 40 magnification (Bar = 100μm). f ChIP experiment with HIF1 alpha antibody on DEN-treated control & Wwox deltaHep (cKO) mice followed by qRT-PCR analysis of HIF1 alpha target genes. * P value " data-badges="" data-application="Immunohistochemistry-Paraffin" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-310202415363727.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - HIF-1alpha & CAIX expression in PDAC & adjacent normal pancreatic tissue. Adjacent normal exocrine pancreas with weak nuclear & cytoplasmic HIF-1alpha staining (a). Adjacent normal pancreatic tissue with weak membranous CAIX staining in the ducts & absent staining in exocrine pancreatic tissue (b). PDAC with weak (intensity 1) nuclear & cytoplasmic HIF-1alpha staining (c). PDAC with strong (intensity 3) nuclear & moderate (intensity 2) cytoplasmic HIF-1alpha staining (d). PDAC with weak to moderate (intensity 1–2) membranous CAIX staining (e). PDAC with strong (intensity 3) membranous CAIX staining (f) Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29973215), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Immunohistochemistry-Paraffin" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-310202415535186.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Chromatin Immunoprecipitation: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Chromatin Immunoprecipitation: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Involvement of CBP in BNIP3 expression regulated by HIF-1 alpha & FOXO3 under hypoxia. (A) UCB-hMSCs were incubated with hypoxia condition for 24 h. Co-immunoprecipitation of HIF-1 alpha & FOXO3 with IgG & CBP were shown in left panel. IgG was used as a negative control. The total protein expressions of HIF-1 alpha, FOXO3, CBP & beta-actin in lysate were shown in right panel. n = 3. (B) CBP (20 μM) was pretreated to UCB-hMSCs, & cells were incubated with hypoxia for 24 h. The BNIP3 mRNA expression level was analyzed by qPCR. n = 6. (C) BNIP3 & beta-actin protein expressions were analyzed by western blot. Data represent mean ± S.E. n = 4. (D, E) Sample DNA was immuno-precipitated with RNA polymerase, IgG, HIF-1 alpha & FOXO3 specific antibodies. CHIP (top panel) & lysate (bottom panel) samples were amplified with the primers of GAPDH & BNIP3 promoters. Quantitative CHIP data was analyzed by qPCR, & shown in the right panel. n = 4. Western blot data were normalized by beta-actin, & qPCR data were normalized by ACTB mRNA expression level. Quantitative data are presented as a mean ± S.E.M. All blot images are representative. *p < 0.05 versus control, #p < 0.05 versus hypoxia. Image collected & cropped by CiteAb from the following publication (https://linkinghub.elsevier.com/retrieve/pii/S2213231717303804), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Chromatin Immunoprecipitation" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-31020241554198.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Lack of HIF-P4H-1 increases the amount of p53. (a) Western blot analysis of p53 in wt & Hif-p4h-1−/− MEFs cultured in 21% or 1% O2 for 24 h. (b,c) Western blot analysis of p53 in Hif-p4h-1 & scrambled (Scrm) siRNA transfected HEK293 cells (b) & wt MEFs treated with 50 µM FG4497 for 24 h (c). (d) qPCR analysis of p53 mRNA in wt & Hif-p4h-1−/− MEFs cultured in 21% or 1% O2 for 24 h. (e) Analysis of p53 protein turnover rate. Hif-p4h-1−/− & wt MEFs were treated with 200 μg/ml of cycloheximide for the indicated time points & whole cell lysates were blotted for p53. (f) Western blot analysis of MDM2 in wt & Hif-p4h-1−/− MEFs cultured in 21% or 1% O2 for 24 h. (g) Western blot analysis of p53 & MDM2 in wt MEFs treated with 10 μM nutlin-3a for 24 h with or without overexpression of human HIF-P4H-1 (OE). (h,i) Western blot analysis of HIF1 alpha in wt & Hif-p4h-1−/− MEFs cultured in 21% or 1% O2 for 24 h (h) & in scrambled & Hif-p4h-1 siRNA transfected HEK293 cells (i). (j) Western blot analysis of ubiquitination of p53 in Hif-p4h-1 & scrambled siRNA transfected HEK293 cells. The cells were transfected with HA-ubiquitin & endogenous p53 was immunoprecipitated followed by Western blotting with anti-HA & anti-P53 antibodies. (k) Western blot analysis of p53 in Hif-p4h-1−/− & wt MEFs were treated with or without 10 μM MG132 for 5 h. Data are presented as representative Western blots & as mean ± s.d., n = at least 3 individual MEF isolates or experiments. *P " data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-310202415541955.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - HIF-1 alpha-mediated repression of ENT1 expression in Caki-1 cells under hypoxia. Comparison of HIF-1 alpha & HIF-2 alpha protein expression in human matched renal normal-tumor samples (n=11) (A) & 3 RCC cell lines (786-O, 769-P, Caki-1) under normoxia or hypoxia for 6 h (B). (C) Schematic diagram of two hypoxia response elements (HRE) in the promoter of ENT1. (D) CHIP-qPCR analysis of HIF-1 alpha at the promoter of ENT1 in Caki-1 cells, exposed under normoxia or hypoxia for 48 h. Two different HIF-1 alpha antibodies (610958, BD Biosciences; NB100-105, Novus) were used to pull down HIF-1 alpha. The protein expression level of HIF-1 alpha (E) & mRNA expression level of SLC29A1 (F) in Caki-1 under normoxia or hypoxia & HIF-1 alpha knockout-Caki-1 cells. (G) The expression level of ARNT1 (HIF-1 beta) was detected in Caki-1, transfected with ARNT1 specific siRNAs or NC. Student's t test (two-tailed) was used. (H, I) The expression level of ENT1 in Caki-1, transfected with ARNT1 specific siRNAs or NC, under normoxia or hypoxia. Oneway-ANOVA analysis was used. The results are expressed as mean ± SEM (n=3). ***P" data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-31020241553389.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - L. donovani infection induces HIF-1 alpha expression in CD11chi splenic DCs in an IRF-5 dependent manner.Mice were infected with 2x107 amastigotes intravenously. (A) Real-time PCR analysis of HIF-1 alpha mRNA expression in CD11c+ cells purified from C57BL/6 mice at various time points after infection. (B) Immunoblot analysis of HIF-1 alpha expression in CD11c+ cells from C57BL/6 mice (upper panel) & densitometric analysis normalized to beta-actin expression & expressed as fold increase to results obtained with naïve mice (lower panel). (C) Real-time PCR analysis of HIF-1 alpha expression in sorted CD11c+ cells from Irf-5flox/floxCre- & Irf-5flox/floxCMV-Cre+. (D) Immunoblot analysis of Hif-1 alpha expression in CD11c+ cells population of Irf5flox/floxCre- (left upper panel) & Irf-5flox/floxCMV-Cre+ (right upper panel), & densitometric analysis normalized to beta-actin expression & expressed as fold increase to results obtained with naïve mice (lower panels). (E) Real-time PCR analysis of Hif-1 alpha expression in CD11c- splenocytes from Irf-5flox/floxCre- & Irf-5flox/floxCMV-Cre+. (F) Real-time PCR analysis of HIF-1 alpha mRNA expression in BMDC from Irf-5flox/floxCMV-Cre+ & Cre- mice. All data represent mean ± SEM combined from 3 independent experiments. Image collected & cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.ppat.1004938), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-310202415532011.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Optical redox ratio (ORR) demonstrates reduced glycolytic phenotype following 24 hours of YC-1 treatment. ORR of A549 & A549RR cells before, 12 & 24 hours following 50 μM YC-1 treatment to inhibit HIF-1 (a,b). Immunoblotting for HIF-1 alpha (c) & PDK-1 (d) before & 24 hours following YC-1 treatment to verify in of HIF-1 alpha. Representative immunoblots were horizontally cropped at the molecular weight indicated (e). Uncropped exposures can be seen in Supplemental Fig. 1. Experiments were performed in triplicate across 3 independent experiments. *p " data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-310202415532019.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - A beta facilitates HIF1 alpha synthesis & autophagy inhibition via mTOR activation. (A) SK-N-MC cells exposed to A beta (5 μM) for 0–48 h. HIF1 alpha & beta-actin expression analyzed by WB. n = 3. (B) Cells pretreated w/ NAC (1 mM) for 30 min prior to A beta treatment for 24 h. HIF1 alpha & beta-actin expression analyzed by WB. n = 3. (C,E) Cells incubated w/ rapamycin (10 nM) for 30 min prior to A beta treatment for 24 h. Phosphorylation of 4EBP1 (Thr 37/46) & 4EBP1, phosphorylation of p70S6K1 (Thr 389), HIF1 alpha & beta-actin analyzed by WB. n = 6. (D) Protein samples immunoprecipitated by eukaryotic translation initiation factor 4E (eIF4E) antibody-conjugated protein A/G agarose beads. Samples blotted w/ 4EBP1 & eIF4E-specific antibodies. n = 3. (F) Cells exposed to PF4708671 (10 μM) for 30 min prior to A beta treatment for 24 h. HIF1 alpha & beta-actin expression detected by WB. n = 6. (G) Cells exposed to cycloheximide (4 μM) for 30 min prior to A beta treatment for 24 h. HIF1 alpha & beta-actin expressions detected by WB. n = 6. (H) Cells pretreated w/ rapamycin (10 nM) for 30 min, incubated w/ A beta for 24 h & analyzed by WB w/ LC3, p62 & beta-actin specific antibodies. n = 3–6. (I) LC3 puncta visualized by confocal microscopy. Presented results merged images. Green & red fluorescents indicate LC3 & PI respectively. Scale bars, 50 μm (magnification × 600). (J) Cells pretreated w/ trehalose (10 μM) for 30 min prior to A beta treatment for 24 h. Cytotoxicity measured by MTT assay at an absorbance of 545 nm using a microplate reader. Data present the mean ± SE. n = 6. (K) Cell viability measured by trypan blue exclusion assay. Data presented as a mean ± SE. n = 6. Each blot image presented as representative image. *p < 0.05 vs. control, #p < 0.05 vs. A beta treatment. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28790888), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-310202415525272.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - FDFT1 inhibits glycolysis through suppressing the AKT-mTOR-HIF1 alpha pathway in CRC.aFDFT1 overexpression reduced glucose uptake in CT26 cells. bFDFT1 overexpression decreased lactate production via glycolysis in CT26 cells. c ECAR reduced when FDFT1 overexpressed in CT26 cells. d OCR increased when FDFT1 overexpressed in CT26 cells. eFDFT1 knockdown increased glucose uptake in CT26 cells. fFDFT1 knockdown increased lactate production via glycolysis in CT26 cells. g ECAR increased when FDFT1 knocked down in CT26 cells. h OCR decreased when FDFT1 knocked down in CT26 cells. i, mFDFT1 overexpression inhibited the protein & mRNA expression of mTOR-targeted glycolytic enzymes, including GLUT1, HK2, PGK1, GPI, & LDHA, in CT26 cells. j, mFDFT1 knockdown increased the protein & mRNA expression of mTOR-targeted glycolytic enzymes, including GLUT1, HK2, PGK1, GPI, & LDHA, in CT26 cells. k, nFDFT1 overexpression decreased the protein & mRNA expression of AKT, mTOR, & HIF1 alpha. l, nFDFT1 knockdown increased protein & mRNA expression of AKT, mTOR, & HIF1 alpha. o Photograph of dissected tumors (the first line: normal diet, the second line: FMD + glucose, the third line: FMD, n = 5). p The tumor volumes measured every 2 days after 13th day. The FMD + glucose group can reverse the tumor growth inhibition induced by the FMD (n = 5; ns: P = 0.1838; P = 0.0001). q The protein level of FDFT1 & mTOR in dissected tumor samples from normal diet group, FMD group & FMD + glucose group measured by western blotting. r The glucose level in these three groups. Error bars, mean ± SD, data are from three independent experiments. Two-sided t tests. *P < 0.05, **P < 0.01, ***P < 0.001, compared w/ the control group (or normal diet group). #P < 0.05, ##P < 0.01. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32313017), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-310202415532069.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - FDFT1 inhibits glycolysis through suppressing AKT-mTOR-HIF1 alpha pathway in CRC.aFDFT1 overexpression reduced glucose uptake in CT26 cells. bFDFT1 overexpression decreased lactate production via glycolysis in CT26 cells. c ECAR reduced when FDFT1 overexpressed in CT26 cells. d OCR increased when FDFT1 overexpressed in CT26 cells. eFDFT1 knockdown increased glucose uptake in CT26 cells. fFDFT1 knockdown increased lactate production via glycolysis in CT26 cells. g ECAR increased when FDFT1 knocked down in CT26 cells. h OCR decreased when FDFT1 knocked down in CT26 cells. i, mFDFT1 overexpression inhibited protein & mRNA expression of mTOR-targeted glycolytic enzymes, including GLUT1, HK2, PGK1, GPI, & LDHA, in CT26 cells. j, mFDFT1 knockdown increased protein & mRNA expression of mTOR-targeted glycolytic enzymes, including GLUT1, HK2, PGK1, GPI, & LDHA, in CT26 cells. k, nFDFT1 overexpression decreased protein & mRNA expression of AKT, mTOR, & HIF1 alpha. l, nFDFT1 knockdown increased protein & mRNA expression of AKT, mTOR, & HIF1 alpha. o Photograph of dissected tumors (the first line: normal diet, second line: FMD + glucose, third line: FMD, n = 5). p The tumor volumes measured every 2 days after 13th day. The FMD + glucose group can reverse tumor growth inhibition induced by FMD (n = 5; ns: P = 0.1838; P = 0.0001). q The protein level of FDFT1 & mTOR in dissected tumor samples from normal diet group, FMD group & FMD + glucose group measured by western blotting. r The glucose level in these three groups. Error bars, mean ± SD, data are from three independent experiments. Two-sided t tests. *P < 0.05, **P < 0.01, ***P < 0.001, compared w/ control group (or normal diet group). #P < 0.05, ##P < 0.01. Image collected & cropped by CiteAb from following publication (https://pubmed.ncbi.nlm.nih.gov/32313017), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-310202415535145.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - L. donovani infection induces HIF-1 alpha expression in CD11chi splenic DCs in an IRF-5 dependent manner.Mice were infected with 2x107 amastigotes intravenously. (A) Real-time PCR analysis of HIF-1 alpha mRNA expression in CD11c+ cells purified from C57BL/6 mice at various time points after infection. (B) Immunoblot analysis of HIF-1 alpha expression in CD11c+ cells from C57BL/6 mice (upper panel) & densitometric analysis normalized to beta-actin expression & expressed as fold increase to results obtained with naïve mice (lower panel). (C) Real-time PCR analysis of HIF-1 alpha expression in sorted CD11c+ cells from Irf-5flox/floxCre- & Irf-5flox/floxCMV-Cre+. (D) Immunoblot analysis of Hif-1 alpha expression in CD11c+ cells population of Irf5flox/floxCre- (left upper panel) & Irf-5flox/floxCMV-Cre+ (right upper panel), & densitometric analysis normalized to beta-actin expression & expressed as fold increase to results obtained with naïve mice (lower panels). (E) Real-time PCR analysis of Hif-1 alpha expression in CD11c- splenocytes from Irf-5flox/floxCre- & Irf-5flox/floxCMV-Cre+. (F) Real-time PCR analysis of HIF-1 alpha mRNA expression in BMDC from Irf-5flox/floxCMV-Cre+ & Cre- mice. All data represent mean ± SEM combined from 3 independent experiments. Image collected & cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.ppat.1004938), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-31020241612666.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - WNT11 is induced by hypoxia or hypoxic mimetics in different cell types.(A) Increased Wnt11 mRNA in EMSC adipocytes (Day 12) after hypoxia-mimetic treatments. EMSC adipocytes were treated with CoCl2 (0.1 mM), DFO (0.1 mM) or DMOG (0.1 mM) for 24 hrs. Values were normalized to Tbp mRNA & are expressed relative to control (n = 3). (B,C) Increased Wnt11 mRNA by hypoxia in EMSC preadipocytes & adipocytes (Day 0–12 after differentiation) (B), & C2C12 myoblast & myocyte (Day 0 & 8 after differentiation) (C). Wnt11 mRNA was assessed by quantitative PCR in cells exposed to air (21% O2) or hypoxia (1% O2) for 24 hrs. (n = 4). Values were normalized to Tbp mRNA & are expressed relative to 21% O2 samples (left panel). (D) Immunoblot analyses of HeLa cells under normal air or hypoxia for 24 hrs. (E,F) Induction of Wnt11 by increasing concentrations of DMOG in MDA-MB-231 cells (E) & 4T1 cells (F). (G) EMSCs treated with 0.1 mM DMOG for the indicated times. Wnt11 & Vegf mRNA expression was measured by qPCR & normalized to Tbp mRNA (n = 4). (H) WNT11 protein levels after DMOG treatment normalized to alpha-Tubulin (upper panel; n = 4). Representative immunoblots of EMSCs treated with 0.1 mM DMOG for the indicated times (Lower panel). (I) Protein expression in MDA-MB-231 cells treated with 0.1 mM DMOG. (J) Induction of Wnt11 promoter activity by hypoxia or hypoxia mimetics. pGL3-Wnt11 promoter plasmid was transfected into C2C12 cells. Cells were incubated with DMOG (left panel, n = 4) or under 21% O2 or 1% O2 (right panel, n = 8) for 24 hrs. For panels (A–C,G,H,J), values are mean ± s.e.m. *p " data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-310202415525213.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - hIAPP upregulates the HIF1 alpha/PFKFB3 stress pathway. a Summary of the differentially expressed genes of interest after microarray analysis25 performed on RNA isolated from wild type (WT) & hIAPP overexpressing (HIP) rat islets (4.5 months) presented as a fold change over WT. Asterisk represents genes that were differentially expressed in one experiment. b LDHA & MCT1 mRNA levels in HIP versus WT as measured by qRT-PCR. c Lactate production rate (fold change) measured in isolated islets from HIP relative to WT. d Representative Western blot of PFKFB3 & HIF1 alpha protein levels in whole cell extracts & nuclear-enriched fractions of islets from 6 months old WT (3) & HIP (3) rats. GAPDH & PARP were used as loading controls for whole cell extract (WCE) and nuclear extracts, respectively. e Quantification of HIF1 alpha (upper panel) & PFKFB3 (lower panel) in cytoplasmic & nuclear fractions. Data are presented as mean ± SEM, n = 3 independent experiments for (b) & (d), & n = 4 independent experiments for (c). Statistical significance was analyzed by Student t-test (*p " data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-31020241554534.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Ccl7 is a HIF1 alpha direct target gene.a qPCR analysis of Ccl7 mRNA expression in Hif1afl/fl & Hif1a deltaSMC VSMCs treated with vehicle, CoCl2, normoxia, or hypoxia (2% O2) for 6, 12, & 24 h. *P " data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-310202415535166.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Hypoxic/necrotic core in GemOE tumors enhances Ac-HMGB1 secretion(A–C) Adjacent sections from a GemOE tumor IHC stained for geminin (A), c-Abl (B) & HMGB1 (C). (D) The level of circulating HMGB1 measured using specific ELISA assay performed on serum isolated from samples collected 7 weeks after mice were injected in mammary fat pads with naïve HME cells (n = 10, no tumors developed) or GemOE cells (n = 30, tumor-bearing, p = 0.00042). Two different sets (E, G, I & K) & (F, H, J & L) of adjacent sections from GemOE orthotopic mammary tumors stained with H & E (E & F), or IHC stained for geminin (G & H), HMGB1 (I & J) as well as HIF-1 alpha (K) or hypoxyprobe (L). N denotes necrosis within these tumors that are shown adjacent to the hypoxic cells as indicated by high HIF-1 alpha or hypoxyprobe staining. These cells are also expressing cytoplasmic HMGB1. (M) The levels of HMGB1 detected using specific ELISA assay released from iGem9, iG197, iG240 or iG257 cells grown under normoxic (N) or hypoxic (H) conditions. Experiments were done in triplicates 3 different times, **represents p < 0.001. (N) The level of acetylated HMGB1 passively diffused from naïve HME, iG197, iG240 or iG257 cells after repeated freeze & thaw cycles. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26989079), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Immunohistochemistry" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-31020241612678.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - A beta induces the expression of cell cycle regulatory proteins. (A) SK-N-MC cells were exposed to A beta (5 μM) for 24 h. The mRNA expression levels of CDK5, P35 & P39 were analyzed by real-time PCR. The mRNA expression level was normalized by beta-actin mRNA expression level. Data represent the mean ± SE. n = 4. (B)hif1 alpha specific- & non-targeting (NT) siRNA were transfected to the cells for 24 h prior to A beta treatment. Cyclin D1, CDK4, cyclin E, CDK2, HIF1 alpha & beta-actin was detected by western blot. n = 3. (C–F) Cells were pretreated with trehalose (10 μM), rapamycin (10 nM), PF4708671 (10 μM) & cycloheximide (4 μM) for 30 min prior to A beta treatment for 24 h. Cyclin D1, CDK4, cyclin E, CDK2 & beta-actin were detected by western blot. n = 3–6. (G) Mouse hippocampal neurons were transfected with hif1 alpha specific- & NT siRNAs for 24 h prior to A beta treatment for 24 h. Samples were blotted with Cyclin D1, CDK4, cyclin E, CDK2 & beta-actin specific antibodies. n = 3–6. (H) Mouse hippocampal neurons were pretreated with trehalose (10 μM) for 30 min & incubated with A beta for 24 h. cyclin D1, CDK4, cyclin E, CDK2, HIF1 alpha & beta-actin were analyzed by western blot. n = 3–6. Data are presented as a mean ± SE. *p < 0.05 vs. control, #p < 0.05 vs. A beta treatment. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28790888), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-3102024155525.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Hypoxia induces expression of WNT11 through VHL.(A,B) Higher basal levels of WNT11 protein in Vhl-deleted cells (lenti-Cre infected Vhlf/f). EMSCs isolated from Vhlf/f mouse were infected with lentivirus carrying either GFP gene (for control) or Cre recombinase (for knockout). Non-infected cells were also used as a control. Immunoblot analysis of control or Vhl KO EMSCs treated with 0.1 mM DMOG (A), & EMSCs exposed to air (21% O2) or hypoxia (1% O2) for 24 hrs (B). Laminin, alpha-tubulin, & lamin A/C were used as loading controls, WNT11 normalized to alpha-Tubulin was shown. (C,D) Inactivation of the Vhl gene results in increased Wnt11 mRNA. Wnt11 & Vegf mRNA levels in liver (C) or duodenum (D) were measured by qPCR in Liver-VhlcKO or duodenum-VhlcKO & control mice (n = 5 per group). Values normalized to Tbp mRNA are expressed relative to tissues from control mice. For panels (C,D), values are mean ± s.e.m. *p " data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-310202415525228.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - QSCs are hypoxic in the niche & express HIF2A, but not HIF1A.(A) Timeline of in vivo pimonidazole labeling in SC-INTACT mice & representative confocal images of uninjured/resting EDL myofibers (n >50 myofibers from n = 3 mice) showing that nmGFP+ QSCs were pimonidazole+. Scale bars: 50 μm & 10 μm (insets). Inset images show that pimonidazole signals were relatively enriched in the cytoplasm of QSCs. Arrowheads indicate a QSC; asterisks indicate a myonucleus. (B) Percentage of pimonidazole+ QSCs. (C) Timeline of in vivo CCI-103F labeling in C57BL/6 mice & representative images of uninjured/resting EDL myofibers (n >50 myofibers from 3 mice) showing that nmGFP+ QSCs were CCI-103F+. Arrowheads indicate a QSC; asterisks indicate a myonucleus. Scale bar: 20 μm. (D) Percentage of CCI-103F+ QSCs. (E & F) Representative images of uninjured/resting EDL myofibers from C57BL/6 mice (n >50 myofibers from 6 mice/group) showing that most QSCs were HIF2A+, but HIF1A–. Scale bars: 10 μm. (G) Percentage of HIF1A+ & HIF2A+ QSCs. Data represent the mean ± SEM. Image collected & cropped by CiteAb from the following publication (https://www.jci.org/articles/view/96208), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Immunocytochemistry/ Immunofluorescence" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-31020241612625.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - FDFT1 inhibits glycolysis through suppressing AKT-mTOR-HIF1 alpha pathway in CRC.aFDFT1 overexpression reduced glucose uptake in CT26 cells. bFDFT1 overexpression decreased lactate production via glycolysis in CT26 cells. c ECAR reduced when FDFT1 overexpressed in CT26 cells. d OCR increased when FDFT1 overexpressed in CT26 cells. eFDFT1 knockdown increased glucose uptake in CT26 cells. fFDFT1 knockdown increased lactate production via glycolysis in CT26 cells. g ECAR increased when FDFT1 knocked down in CT26 cells. h OCR decreased when FDFT1 knocked down in CT26 cells. i, mFDFT1 overexpression inhibited protein & mRNA expression of mTOR-targeted glycolytic enzymes, including GLUT1, HK2, PGK1, GPI, & LDHA, in CT26 cells. j, mFDFT1 knockdown increased protein & mRNA expression of mTOR-targeted glycolytic enzymes, including GLUT1, HK2, PGK1, GPI, & LDHA, in CT26 cells. k, nFDFT1 overexpression decreased protein & mRNA expression of AKT, mTOR, & HIF1 alpha. l, nFDFT1 knockdown increased protein & mRNA expression of AKT, mTOR, & HIF1 alpha. o Photograph of dissected tumors (the first line: normal diet, second line: FMD + glucose, third line: FMD, n = 5). p The tumor volumes measured every 2 days after 13th day. The FMD + glucose group can reverse tumor growth inhibition induced by FMD (n = 5; ns: P = 0.1838; P = 0.0001). q The protein level of FDFT1 & mTOR in dissected tumor samples from normal diet group, FMD group & FMD + glucose group measured by western blotting. r The glucose level in these three groups. Error bars, mean ± SD, data are from three independent experiments. Two-sided t tests. *P < 0.05, **P < 0.01, ***P < 0.001, compared w/ control group (or normal diet group). #P < 0.05, ##P < 0.01. Image collected & cropped by CiteAb from following publication (https://pubmed.ncbi.nlm.nih.gov/32313017), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-31020241612647.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Hypoxia induces expression of WNT11 through VHL.(A,B) Higher basal levels of WNT11 protein in Vhl-deleted cells (lenti-Cre infected Vhlf/f). EMSCs isolated from Vhlf/f mouse were infected with lentivirus carrying either GFP gene (for control) or Cre recombinase (for knockout). Non-infected cells were also used as a control. Immunoblot analysis of control or Vhl KO EMSCs treated with 0.1 mM DMOG (A), & EMSCs exposed to air (21% O2) or hypoxia (1% O2) for 24 hrs (B). Laminin, alpha-tubulin, & lamin A/C were used as loading controls, WNT11 normalized to alpha-Tubulin was shown. (C,D) Inactivation of the Vhl gene results in increased Wnt11 mRNA. Wnt11 & Vegf mRNA levels in liver (C) or duodenum (D) were measured by qPCR in Liver-VhlcKO or duodenum-VhlcKO & control mice (n = 5 per group). Values normalized to Tbp mRNA are expressed relative to tissues from control mice. For panels (C,D), values are mean ± s.e.m. *p " data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-310202415532090.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Role of HIF-1 alpha, FOXO3 in hypoxia-induced BNIP3 expression. (A) UCB-hMSCs were incubated with various durations of hypoxia (0–48 h). The protein expressions of HIF-1 alpha & beta-actin were detected by western blot. n = 4. (B) UCB-hMSCs were pretreated with NAC (5 mM) for 30 min prior to hypoxia incubation for 24 h. The protein expressions of HIF-1 alpha, lamin A/C & beta-tubulin in non-nuclear & nuclear fractionized cell samples were assessed by using western blot. n = 3. (C) UCB-hMSCs were immuno-stained with HIF-1 alpha & PI (magnification ×600). Scale bars, 37.5 µm. (D) HIF1A siRNA or NT siRNA was transfected to cells prior to hypoxia treatment for 24 h. The mRNA expression of BNIP3 was analyzed by qPCR. n = 6. (E) The protein expressions of BNIP3 & HIF-1 alpha were detected by western blot. n = 4. (F) NAC (5 mM) was pretreated to UCB-hMSCs prior to hypoxia treatment for 24 h. FOXO3, lamin A/C & beta-tubulin proteins expressions were assessed by western blot. n = 3. (G) FOXO3 siRNA transfected to UCB-hMSCs prior to hypoxia treatment for 24 h. The FOXO3 mRNA expression was measured by qPCR. n = 6. (H) BNIP3, FOXO3 & beta-actin expressions were detected by western blot. n = 3. Western blot data were normalized by beta-actin, & qPCR data were normalized by ACTB mRNA expression level. Lamin A/C & beta-tubulin were used as nuclear & non-nuclear protein controls, respectively. Quantitative data are presented as a mean ± S.E.M. All blots & confocal images are representative. *p < 0.05 versus control, #p < 0.05 versus hypoxia. Image collected & cropped by CiteAb from the following publication (https://linkinghub.elsevier.com/retrieve/pii/S2213231717303804), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-31020241552521.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - ROS overproduction mediated apoptosis induced by combined treatment of DDP & HIF-1 alpha in PC-3 xenografts & cell culture. (a) ROS monitored by DHE (red) & nuclei by DAPI (blue) staining in PCa xenografts (scale bars, 50 µm). (b) MDA formation of PCa xenografts was examined after various treatments (c,d). PC-3 cells were treated with DDP, si-HIF-1 alpha plasmid, or both, in the presence or absence of NAC (5 mM) or DHLA (0.25 mM) for 24 h. Total lysates (c) & culture media (d) were used to detect cellular H2O2 level. (e,f) Western analysis for HIF-1 alpha as well as cleaved caspase-3 & PARP in PC-3 cells following various treatments. Data were presented as mean ± SD of three independent experiments. *p " data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-310202415525236.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - miR-128 downmodulation regulates MAFG increase in hypoxic condition both in vitro & in vivo. C2C12 cells exposed to hypoxia (2% O2) for 4 h, & miR-128 levels (a) determined by RT-qPCR on total RNAs after normalization w/ the small RNU6. Data are expressed as relative to values obtained in untreated control which set equal to 1. Each column in the panels represents the mean ± SD of 3 independent experiments. ∗p < 0.05. Western blotting analysis (b) of HIF-1 alpha, MAFG, & HMOX-1 performed on total extracts from the same cells, as described in Materials & Methods. Tubulin used as a loading control. mRNA levels of HMOX-1 & x-CT genes (c) determined by RT-qPCR on total RNAs, & relative changes calculated by comparing treated cells versus control, after normalization w/ c-ABL. Data reported as relative to values obtained in normoxia, which set equal to 1. Each column in the panel represents the mean ± SD of 3 independent experiments. ∗p < 0.05. Representative laser Doppler analysis (d) of blood flow before & 4 hours after (post 4 h) hindlimb ischemia procedure (n = 3-4 mice/group). Representative Western blot analysis & densitometric analysis (e) of MAFG & HMOX-1 protein levels performed on hindlimb muscle lysates from littermates after sham procedure (sham) or not-ischemic limb (NIL) & ischemic limb (IL) 4 h after femoral artieriectomy. Tubulin protein levels used as loading controls (∗p < 0.05 versus sham; n = 3-4 hindlimb/group). miR-128 levels (f) determined by RT-qPCR on total RNAs after normalization w/ the small RNU6. The values obtained in not-ischemic limb (NIL) set equal to 1. The data are expressed as the mean ± standard error & are representative of 3 independent experiments. ∗p < 0.05. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29138682), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-310202415532060.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Effect of ENMD-1198 on HIF-1 alpha & VEGF expression in HCC cells. To determine effects of ENMD-1198 on nuclear HIF-1 alpha expression, HUH-7 cells were incubated for 16 h ± ENMD-1198 & subsequently exposed to hypoxia (1% O2, 4 h). A) Western blot analysis of whole protein showed that ENMD-1198 effectively blunted hypoxic induction of HIF-1 alpha protein. B) In addition, treatment with ENMD-1198 down-regulated constitutive VEGF-A mRNA levels in HCC cells (HepG2), as measured by real-time PCR (* P < 0.01) (n = 3/group). VEGF-A mRNA expression is normalized to beta-actin. C) ELISA analysis for VEGF in culture supernatants (HepG2). Hypoxia (20 h, 1% O2) markedly increased VEGF protein. ENMD-1198 treatment lowered VEGF secretion under hypoxic conditions (*P < 0.05). D) Western blot analysis for VEGF. Cells were incubated under either non-hypoxic, or hypoxic conditions ± ENMD-1198. Treatment with ENMD-1198 slightly lowered the hypoxic induction of VEGF, as determined by densitometry. Bars: SEM. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/18651980), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-31020241683923.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Effects of resveratrol on the expression of SIRT1, PGC-1 alpha, HIF-1 alpha & apoptosis proteins in vitro. (A) The expression of SIRT1, PGC-1 alpha, & Bcl-2 proteins were lower in iohexol treated HK-2 cells compared with control cells, which was reversed by treatment with resveratrol. The expression of HIF-1 alpha, Bax, cleaved caspase-3, & Cyt-C were markedly increased after iohexol injection, which was reversed by treatment with resveratrol. Furthermore, this effect was enhanced by HIF-1 alpha inhibitor, 2-MeOE2. (B–H) Relative densitometry of SIRT1–PGC-1 alpha–HIF-1 alpha signaling proteins expressed as mean ± standard error. *P < 0.05 vs. Cont; #P < 0.05 vs. PC–AKI. Cont, control; Res, resveratrol; PC–AKI, post-contrast acute kidney injury; SIRT1, silent information regulator l; HIF-1 alpha, hypoxia-inducible transcription factor-1 alpha; Cyt-C, cytochrome C; PGC-1 alpha, peroxisome proliferator-activated receptor gamma coactivator-1 alpha; Ex527, SIRT1 specific inhibitor; LG, low glucose. Groups: Res, HG+res+iohexol; Ex527, HG+Ex527+iohexol; PC–AKI, HG+iohexol; 2-MeOE2, HG+res+2-MeOE2+iohexol; Cont, LG. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31402864), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-31020241612610.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Upregulation of HIF-1 alpha in human PCa. (a) HIF-1 alpha protein was detected by western blot in nonmalignant (RWPE-1 & BPH1) & PCa cell lines (PC-3, DU145, LNCaP, & 22RV1) as indicated. (b–d) Total RNA extracted from RWPE-1, BPH1, PC-3, DU145, LNCaP, & 22RV1 cells was subjected to qRT-PCR for HIF-1 alpha (b), VEGF (c) & GLUT4 (d). (e) The HIF-1 alpha promoter-driven reporter (firefly luciferase) & a control vector (Renilla luciferase) were co-transfected into RWPE-1, BPH1, PC-3, DU145, LNCaP, & 22RV1 cells for measurement of luciferase activity. HIF-1 alpha promoter activity was calculated as a ratio of firefly to Renilla activity. (f) Human normal & malignant tissue (Gleason score 9) sections were probed with HIF-1 alpha antibody (scale bars, 100 µm). (g) Protein expression of HIF-1 alpha, VEGF, & GLUT4 were examined with western blot, in PC-3, DU145, & LNCaP cells after various treatments as indicated. Data are expressed as mean ± SD of seven independent experiments. $p " data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-31020241554522.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Cx43-overexpressing cells regulate the expression of HIF-1 alpha, vascular endothelial growth factor (VEGF), & the proliferation of endothelial cells. (a) The expression of Cx43 & HIF-1 alpha was measured in Cx43-overexpressing cells by Western blot analysis. beta-actin expression served as loading controls for & total protein. Inserted values indicated relative proteins expression in comparison with beta-actin; (b) Cx43-overexpressing cells were cotransfected with pCLNCX-6× HRELuc & pTCYLacZ plasmids. At 6 h post-transfection, their luciferase activities were determined & normalized with beta-gal activity. Data shown were the mean ± SD (n = 4); (c) The conditioned medium of Cx43-overexpressing cells was measured by ELISA. Data shown were the mean ± SD (n = 4); & (d) The conditioned medium of Cx43-overexpressing cells reduced the proliferation of endothelial cells. The HEMC-1 cells treated with conditioned medium of Cx43-overexpressing cells were examined the proliferation activity. Cell viability was assessed by the WST-1 assay. Data shown were the mean ± SD (n = 4). * p < 0.05; *** p < 0.001. Image collected & cropped by CiteAb from the following publication (http://www.mdpi.com/1422-0067/16/1/439), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-310202415523943.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Prolonged hypoxia increases glutamine metabolism in PDAC cells, & HIF‐2 alpha can promote non‐canonical glutamine metabolism in chronic hypoxic conditions. (A) Time course of glutamine consumption at 1%, 3% or 20% O2, each time data point is an average of triplicate experiments. (B) Panc‐1 & Capan‐2 were incubated for 48 hrs at 1%, 3% or 20% O2, GLS1, GOT1 & GOT2 protein were measured by Western blot. beta‐Actin was used as loading control. (C) Panc‐1 & Capan‐2 were incubated for 48 hrs at 1%, 3% or 20% O2, GLS1, GOT1 & GOT2 mRNA were measured by qRT‐PCR. Data are presented as mean ± S.D. from three independent experiments. (D & F) Si‐HIF‐2 alpha‐transfected Panc‐1 & Capan‐2 cultured at 1% or 3%O2 for 48 hrs. The level of HIF‐2 alpha & glutamine metabolism enzymes mRNA & protein were determined by qRT‐PCR (mRNA) & Western blot (protein), & beta‐actin was used as loading control. Data are presented as mean ± S.D. from three independent experiments. *P < 0.05, **P < 0.01. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28544376), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-310202415523937.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - HIF1 alpha & PFKFB3 are protective against hIAPP mediated beta-cell death. a Representative immunoblotting (left) & quantification (right) of the whole cell extract (WCE) from asynchronous INS 832/13 cells treated with either HIF1 alpha inhibitor KC7F2 (10 μM) or PFKFB3 siRNA (75 nM) for 56 h versus control. Cells were transduced with hIAPP adenovirus (75 MOI) or LacZ adenovirus as control (75 MOI) for last 32 h of culture. DMSO or CTRL siRNA, respectively were added to LacZ or hIAPP to equal DMSO or PFKFB3 siRNA used in experimental groups. GAPDH was used as a loading control. Data are presented as mean ± SEM, n = 4, *p " data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-31020241612694.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Immunohistochemical & Western blot analysis of HIF-1 alpha & VEGF expression in OVA-challenged mice.(A & B) Immunostaining for HIF-1 alpha & VEGF in nasal mucosa following the last challenge. Positive HIF-1 alpha signals are brown & are predominantly nuclear & VEGF is predominantly cytoplasmic (magnification 400×, scale bar = 20 µm). (C & D) Representative Western blot analysis showing HIF-1 alpha & VEGF (with beta-actin as a loading control) expression in the nasal mucosa 24 h after the last challenge. All densitometric analyses are presented as the relative ratio of each molecule to beta-actin & the ratio in negative control mice was set to 100. Values represent means±SEM (n = 6 mice). *Significantly different from negative control, p" data-badges="" data-application="Immunohistochemistry" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-31020241552399.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Quantification of MAOB, HiF-1 alpha, & GFAP levels in gliomas(A) Relative levels of MAOB in normal brain, five anaplastic astrocytomas (AA), a geministic astrocytoma (Gem), an astroblastoma (Ablast), & 20 GBM tumors, with the GBM tumors arranged from lowest to highest signal. The same left-right arrangement of GBM tumors is also used in (B) & (C). (B) Relative levels of HiF-1 alpha. (C) Relative levels of GFAP. (D) Correlation between MAOB & HiF-1 alpha in gliomas & GBMs. (E) Correlation between MAOB & GFAP in gliomas & GBMs. (F) Correlation between HiF-1 alpha & GFAP. The coefficient of determination, R2, & p values are shown on all correlation plots. All points are mean ± SD, with n = 4 for tumor samples & n = 12 for the control brain samples. Image collected & cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.6582), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Immunohistochemistry" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-310202415523933.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - DC101, evofosfamide, & HIF-1 alpha shRNA treatment of HT1080 sarcomasA. Western blot of HT1080 cells stably transduced with HIF-1 alpha shRNA (sh.HIF-1 alpha) or scrambled control shRNA (sh.Scr) & grown in 21% oxygen or 1% oxygen. beta-actin serves as loading control. B. Growth of HT1080 cells stably transduced with sh.HIF-1 alpha or sh.Scr in athymic nude mice. Groups were treated with control IgG 20 mg/kg 3 times per week, DC101 20 mg/kg 3 times per week, and/or evofosfamide (Evo) 50 mg/kg 5 times per week. C. Photos of H&E staining, cleaved caspase 3 IF, cleaved caspase 3 & CD31 IF, CD31 IHC, pimonidazole IHC, & HIF-1 alpha IHC. D. Graphs of microvessel density, pimondizale area, & nuclear HIF-1a expression. Bars represent standard deviation. **p < 0.05 compared to all other groups. Image collected & cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.10212), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Immunohistochemistry" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-310202415535163.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - WNT11 is induced by hypoxia or hypoxic mimetics in different cell types.(A) Increased Wnt11 mRNA in EMSC adipocytes (Day 12) after hypoxia-mimetic treatments. EMSC adipocytes were treated with CoCl2 (0.1 mM), DFO (0.1 mM) or DMOG (0.1 mM) for 24 hrs. Values were normalized to Tbp mRNA & are expressed relative to control (n = 3). (B,C) Increased Wnt11 mRNA by hypoxia in EMSC preadipocytes & adipocytes (Day 0–12 after differentiation) (B), & C2C12 myoblast & myocyte (Day 0 & 8 after differentiation) (C). Wnt11 mRNA was assessed by quantitative PCR in cells exposed to air (21% O2) or hypoxia (1% O2) for 24 hrs. (n = 4). Values were normalized to Tbp mRNA & are expressed relative to 21% O2 samples (left panel). (D) Immunoblot analyses of HeLa cells under normal air or hypoxia for 24 hrs. (E,F) Induction of Wnt11 by increasing concentrations of DMOG in MDA-MB-231 cells (E) & 4T1 cells (F). (G) EMSCs treated with 0.1 mM DMOG for the indicated times. Wnt11 & Vegf mRNA expression was measured by qPCR & normalized to Tbp mRNA (n = 4). (H) WNT11 protein levels after DMOG treatment normalized to alpha-Tubulin (upper panel; n = 4). Representative immunoblots of EMSCs treated with 0.1 mM DMOG for the indicated times (Lower panel). (I) Protein expression in MDA-MB-231 cells treated with 0.1 mM DMOG. (J) Induction of Wnt11 promoter activity by hypoxia or hypoxia mimetics. pGL3-Wnt11 promoter plasmid was transfected into C2C12 cells. Cells were incubated with DMOG (left panel, n = 4) or under 21% O2 or 1% O2 (right panel, n = 8) for 24 hrs. For panels (A–C,G,H,J), values are mean ± s.e.m. *p " data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-310202415541970.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - CAPE inhibits MCT-induced HIF-1 alpha expression & PDGF-BB production in rats. (A) Rats were treated with PBS or CAPE (5 or 10 mg/kg) from days 14–28 after MCT treatment 14 days (60 mg/kg). Preparation of lung tissues was analyzed by Western blot to determine the levels of HIF-1 alpha protein. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)was used as a loading control. (B) Serum levels of PDGF-BB in rat was assayed by an ELISA kit. (C) Representative images of immunohistochemical staining for HIF-1 alpha in lung sections from the rats. Scale bars, 50 μm. (D) Immunofluorescence staining of alpha-smooth muscle actin ( alpha-SMA) & HIF-1 alpha in lung sections from rats. Scale bars, 50 μm. Data in (A,B) are expressed as mean ± SEM of three independent experiments. *** p < 0.01, as compared with the PBS group. ### p < 0.01, as compared with the rats exposed to MCT alone. Results are representative of three rats per experimental group. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30909527), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-310202415532043.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - mTOR expression is inversely correlated with FDFT1 expression.aFDFT1 overexpression inhibited the protein level of mTOR, whereas FDFT1 knockdown increased the protein level of mTOR in CT26 & SW620 cells. bFDFT1 expression & mTOR expression were negatively correlated in CRC patient samples. Scale bar: 200 µm. c The mTOR silencing efficiency of the siRNA in CT26 & SW620 cells was validated by western blotting. d The effect of mTOR silencing on FDFT1 expression level in CT26 was evaluated by western blotting. e The protein level of mTOR when FDFT1 knockdown combined with or without mTOR inhibitor in CT26. f The protein level of pS6k, S6k, pS6, & S6 under the effect of fasting & FDFT1 overexpression in CRC cells. g, h CCK8 proliferation assays showed that the silencing of mTOR decreased the proliferation of CT26 & SW620 cells. k, n The silencing of mTOR reduced glucose uptake & lactate production in CT26 & SW620 cells. i, j, l, o The silencing of mTOR decreased the ECAR & increased the OCR in CT26 & SW620 cells. m The silencing of mTOR decreased the expression of AKT, HIF1 alpha, & proteins encoded by relevant glycolytic genes, such as GLUT1, HK2, LDHA, GPI, PGK1, in CT26 & SW620 cells. p–t Based on TCGA data set analysis, mTOR expression was positively correlated with AKT1, HIF1 alpha, GLUT1, HK2, & LDHA expression. Error bars, mean ± SD, the data are from three independent experiments. Two-sided t tests. *P " data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-310202415535192.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Chromatin Immunoprecipitation: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Chromatin Immunoprecipitation: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - HIF-1 alpha-mediated repression of ENT1 expression in Caki-1 cells under hypoxia. Comparison of HIF-1 alpha & HIF-2 alpha protein expression in human matched renal normal-tumor samples (n=11) (A) & 3 RCC cell lines (786-O, 769-P, Caki-1) under normoxia or hypoxia for 6 h (B). (C) Schematic diagram of two hypoxia response elements (HRE) in the promoter of ENT1. (D) CHIP-qPCR analysis of HIF-1 alpha at the promoter of ENT1 in Caki-1 cells, exposed under normoxia or hypoxia for 48 h. Two different HIF-1 alpha antibodies (610958, BD Biosciences; NB100-105, Novus) were used to pull down HIF-1 alpha. The protein expression level of HIF-1 alpha (E) & mRNA expression level of SLC29A1 (F) in Caki-1 under normoxia or hypoxia & HIF-1 alpha knockout-Caki-1 cells. (G) The expression level of ARNT1 (HIF-1 beta) was detected in Caki-1, transfected with ARNT1 specific siRNAs or NC. Student's t test (two-tailed) was used. (H, I) The expression level of ENT1 in Caki-1, transfected with ARNT1 specific siRNAs or NC, under normoxia or hypoxia. Oneway-ANOVA analysis was used. The results are expressed as mean ± SEM (n=3). ***P" data-badges="" data-application="Chromatin Immunoprecipitation" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-310202415525216.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Upregulation of HIF-1 alpha in human PCa. (a) HIF-1 alpha protein was detected by western blot in nonmalignant (RWPE-1 & BPH1) & PCa cell lines (PC-3, DU145, LNCaP, & 22RV1) as indicated. (b–d) Total RNA extracted from RWPE-1, BPH1, PC-3, DU145, LNCaP, & 22RV1 cells was subjected to qRT-PCR for HIF-1 alpha (b), VEGF (c) & GLUT4 (d). (e) The HIF-1 alpha promoter-driven reporter (firefly luciferase) & a control vector (Renilla luciferase) were co-transfected into RWPE-1, BPH1, PC-3, DU145, LNCaP, & 22RV1 cells for measurement of luciferase activity. HIF-1 alpha promoter activity was calculated as a ratio of firefly to Renilla activity. (f) Human normal & malignant tissue (Gleason score 9) sections were probed with HIF-1 alpha antibody (scale bars, 100 µm). (g) Protein expression of HIF-1 alpha, VEGF, & GLUT4 were examined with western blot, in PC-3, DU145, & LNCaP cells after various treatments as indicated. Data are expressed as mean ± SD of seven independent experiments. $p " data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-310202415532057.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Upregulation of HIF-1 alpha in the spleen restricts CD8+ T cell expansion.(A-B) Mice were infected with 2x107 amastigotes intravenously & euthanized at various time points after infection. HIF-1 alpha expression in splenocytes was assessed by real-time PCR (A) & immunoblot analysis (B). (C-F) 2x104 OT-I CD8+ T cells were adoptively transferred into recipient mice a day prior to infection with ovalbumin-transgenic (PINK) L. donovani amastigotes. (C) Graphs represent the average number of OT-I CD8+ T cells found in the spleen from Hif1aflox/flox & Hif1a+/-mice at d7 & d14 p.i.. (D) Percentage of OT-I CD8+ T cells at d7 & d14 p.i.. (E) Percentage of gated OT-I CD8+ expressing CD62Llo/int & negative for CD127. (F) Percentage of OT-I CD8+ T cells that were CD44+ CD127+CD62Lhi. All data represent mean ± SEM of one of 3 independent experiments, n = 5. * denotes p" data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-31020241682171.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Effect of 24 hr exposure to fatty acids on expression of proteins involved in energy metabolism.A) Representative western blots, B) Lactate dehydrogenase A (LDHA), C) Phosphoglycerate mutase 1 (PGAM1), D) Hypoxia inducible factor 1 alpha (HIF1 alpha), E) Isocitrate dehydrogenase (IDH), F) phospho Acetyl CoA carboxylase (ACC)/ACC protein expression. n = 5–6 Protein expression was measured in human bone marrow mesenchymal stem cells (BMMSCs) treated for 24 hr with indicated treatments. The BSA group was treated with media supplemented with 0.55 mM albumin. All fatty acid treated groups were also treated with media supplemented with 0.55 mM albumin in addition to the type & amount of fatty acid indicated in the figures. Values are shown as mean ± SEM. Image collected & cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0120257), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-310202415535135.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - A beta facilitates HIF1 alpha synthesis & autophagy inhibition via mTOR activation. (A) SK-N-MC cells exposed to A beta (5 μM) for 0–48 h. HIF1 alpha & beta-actin expression analyzed by WB. n = 3. (B) Cells pretreated w/ NAC (1 mM) for 30 min prior to A beta treatment for 24 h. HIF1 alpha & beta-actin expression analyzed by WB. n = 3. (C,E) Cells incubated w/ rapamycin (10 nM) for 30 min prior to A beta treatment for 24 h. Phosphorylation of 4EBP1 (Thr 37/46) & 4EBP1, phosphorylation of p70S6K1 (Thr 389), HIF1 alpha & beta-actin analyzed by WB. n = 6. (D) Protein samples immunoprecipitated by eukaryotic translation initiation factor 4E (eIF4E) antibody-conjugated protein A/G agarose beads. Samples blotted w/ 4EBP1 & eIF4E-specific antibodies. n = 3. (F) Cells exposed to PF4708671 (10 μM) for 30 min prior to A beta treatment for 24 h. HIF1 alpha & beta-actin expression detected by WB. n = 6. (G) Cells exposed to cycloheximide (4 μM) for 30 min prior to A beta treatment for 24 h. HIF1 alpha & beta-actin expressions detected by WB. n = 6. (H) Cells pretreated w/ rapamycin (10 nM) for 30 min, incubated w/ A beta for 24 h & analyzed by WB w/ LC3, p62 & beta-actin specific antibodies. n = 3–6. (I) LC3 puncta visualized by confocal microscopy. Presented results merged images. Green & red fluorescents indicate LC3 & PI respectively. Scale bars, 50 μm (magnification × 600). (J) Cells pretreated w/ trehalose (10 μM) for 30 min prior to A beta treatment for 24 h. Cytotoxicity measured by MTT assay at an absorbance of 545 nm using a microplate reader. Data present the mean ± SE. n = 6. (K) Cell viability measured by trypan blue exclusion assay. Data presented as a mean ± SE. n = 6. Each blot image presented as representative image. *p < 0.05 vs. control, #p < 0.05 vs. A beta treatment. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28790888), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-310202415541951.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Ccl7 is a HIF1 alpha direct target gene.a qPCR analysis of Ccl7 mRNA expression in Hif1afl/fl & Hif1a deltaSMC VSMCs treated with vehicle, CoCl2, normoxia, or hypoxia (2% O2) for 6, 12, & 24 h. *P " data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-310202415541926.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - HIF1 alpha drives the expression of PFKFB3 in beta-cells. a Luciferase assay showing the activation of PFKFB3 promoter containing 2 hypoxia elements. INS 832/13 overexpressing LacZ or hIAPP were transfected for 36 h with plasmid vectors containing: RenSP luciferase gene without a promoter (empty vector—EV) measuring the background signal or housekeeping gene promoter driving the expression of RenSP luciferase gene ( beta-actin) or PFKFB3 promoter with hypoxia elements driving the expression of RenSP luciferase gene (PFKFB3). b Representative Western blot of HIF1 alpha, PFKFB3, LDHA, Cdh1 in whole cell extracts of INS 832/13 overexpressing LacZ (CTRL) or hIAPP, silenced or not with short hairpin RNA for PFKFB3 (PFKFB3 shRNA) & HIF1 alpha (HIF1 alpha shRNA) for 36 h. CTRL shRNA is non-target shRNA control vector. beta-actin & GAPDH were used as loading control. Data are presented as mean ± SEM, n = 9 independent biological samples (in (a)). Statistical significance was analyzed by unpaired t-test (*p " data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-310202415525295.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - WNT11 is induced by hypoxia or hypoxic mimetics in different cell types.(A) Increased Wnt11 mRNA in EMSC adipocytes (Day 12) after hypoxia-mimetic treatments. EMSC adipocytes were treated with CoCl2 (0.1 mM), DFO (0.1 mM) or DMOG (0.1 mM) for 24 hrs. Values were normalized to Tbp mRNA & are expressed relative to control (n = 3). (B,C) Increased Wnt11 mRNA by hypoxia in EMSC preadipocytes & adipocytes (Day 0–12 after differentiation) (B), & C2C12 myoblast & myocyte (Day 0 & 8 after differentiation) (C). Wnt11 mRNA was assessed by quantitative PCR in cells exposed to air (21% O2) or hypoxia (1% O2) for 24 hrs. (n = 4). Values were normalized to Tbp mRNA & are expressed relative to 21% O2 samples (left panel). (D) Immunoblot analyses of HeLa cells under normal air or hypoxia for 24 hrs. (E,F) Induction of Wnt11 by increasing concentrations of DMOG in MDA-MB-231 cells (E) & 4T1 cells (F). (G) EMSCs treated with 0.1 mM DMOG for the indicated times. Wnt11 & Vegf mRNA expression was measured by qPCR & normalized to Tbp mRNA (n = 4). (H) WNT11 protein levels after DMOG treatment normalized to alpha-Tubulin (upper panel; n = 4). Representative immunoblots of EMSCs treated with 0.1 mM DMOG for the indicated times (Lower panel). (I) Protein expression in MDA-MB-231 cells treated with 0.1 mM DMOG. (J) Induction of Wnt11 promoter activity by hypoxia or hypoxia mimetics. pGL3-Wnt11 promoter plasmid was transfected into C2C12 cells. Cells were incubated with DMOG (left panel, n = 4) or under 21% O2 or 1% O2 (right panel, n = 8) for 24 hrs. For panels (A–C,G,H,J), values are mean ± s.e.m. *p " data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-3102024165710.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - CAPE inhibits MCT-induced HIF-1 alpha expression & PDGF-BB production in rats. (A) Rats were treated with PBS or CAPE (5 or 10 mg/kg) from days 14–28 after MCT treatment 14 days (60 mg/kg). Preparation of lung tissues was analyzed by Western blot to determine the levels of HIF-1 alpha protein. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)was used as a loading control. (B) Serum levels of PDGF-BB in rat was assayed by an ELISA kit. (C) Representative images of immunohistochemical staining for HIF-1 alpha in lung sections from the rats. Scale bars, 50 μm. (D) Immunofluorescence staining of alpha-smooth muscle actin ( alpha-SMA) & HIF-1 alpha in lung sections from rats. Scale bars, 50 μm. Data in (A,B) are expressed as mean ± SEM of three independent experiments. *** p < 0.01, as compared with the PBS group. ### p < 0.01, as compared with the rats exposed to MCT alone. Results are representative of three rats per experimental group. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30909527), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Immunocytochemistry/ Immunofluorescence" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-310202415523924.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Quantification of MAOB, HiF-1 alpha, & GFAP levels in gliomas(A) Relative levels of MAOB in normal brain, five anaplastic astrocytomas (AA), a geministic astrocytoma (Gem), an astroblastoma (Ablast), & 20 GBM tumors, with the GBM tumors arranged from lowest to highest signal. The same left-right arrangement of GBM tumors is also used in (B) & (C). (B) Relative levels of HiF-1 alpha. (C) Relative levels of GFAP. (D) Correlation between MAOB & HiF-1 alpha in gliomas & GBMs. (E) Correlation between MAOB & GFAP in gliomas & GBMs. (F) Correlation between HiF-1 alpha & GFAP. The coefficient of determination, R2, & p values are shown on all correlation plots. All points are mean ± SD, with n = 4 for tumor samples & n = 12 for the control brain samples. Image collected & cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.6582), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Immunohistochemistry" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-310202415535122.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Role of HIF-1 alpha, FOXO3 in hypoxia-induced BNIP3 expression. (A) UCB-hMSCs were incubated with various durations of hypoxia (0–48 h). The protein expressions of HIF-1 alpha & beta-actin were detected by western blot. n = 4. (B) UCB-hMSCs were pretreated with NAC (5 mM) for 30 min prior to hypoxia incubation for 24 h. The protein expressions of HIF-1 alpha, lamin A/C & beta-tubulin in non-nuclear & nuclear fractionized cell samples were assessed by using western blot. n = 3. (C) UCB-hMSCs were immuno-stained with HIF-1 alpha & PI (magnification ×600). Scale bars, 37.5 µm. (D) HIF1A siRNA or NT siRNA was transfected to cells prior to hypoxia treatment for 24 h. The mRNA expression of BNIP3 was analyzed by qPCR. n = 6. (E) The protein expressions of BNIP3 & HIF-1 alpha were detected by western blot. n = 4. (F) NAC (5 mM) was pretreated to UCB-hMSCs prior to hypoxia treatment for 24 h. FOXO3, lamin A/C & beta-tubulin proteins expressions were assessed by western blot. n = 3. (G) FOXO3 siRNA transfected to UCB-hMSCs prior to hypoxia treatment for 24 h. The FOXO3 mRNA expression was measured by qPCR. n = 6. (H) BNIP3, FOXO3 & beta-actin expressions were detected by western blot. n = 3. Western blot data were normalized by beta-actin, & qPCR data were normalized by ACTB mRNA expression level. Lamin A/C & beta-tubulin were used as nuclear & non-nuclear protein controls, respectively. Quantitative data are presented as a mean ± S.E.M. All blots & confocal images are representative. *p < 0.05 versus control, #p < 0.05 versus hypoxia. Image collected & cropped by CiteAb from the following publication (https://linkinghub.elsevier.com/retrieve/pii/S2213231717303804), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-310202415535175.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Cx43-knockdown cells regulate the expression of HIF-1 alpha, c-Src, VEGF & the proliferation of endothelial cells. (a) The expression of Cx43, c-Src, & HIF-1 alpha was measured in Cx43-knocknown cells by Western blot analysis. beta-actin expression served as loading controls for & total protein. Inserted values indicated relative protein expression in comparison with beta-actin; (b) Cx43-knockdown cells were cotransfected with pCLNCX-6× HRELuc & pTCYLacZ plasmids. Then, cells were treated with 100 ng/μL PP2 or DMSO for 1 h. At 6 h post-transfection, their luciferase activities were determined & normalized with beta-gal activity. Data shown were the mean ± SD (n = 4); (c) The conditioned medium of Cx43-knockdown cells was measured by ELISA. Data shown were the mean ± SD (n = 4); (d) The conditioned medium of Cx43-knockdown cells reduced the proliferation of endothelial cells. The HEMC-1 cells treated with conditioned medium of Cx43-knockdown cells were examined for proliferation activity. Cell viability was assessed by the WST-1 assay. Data shown were the mean ± SD (n = 4). * p < 0.05; ** p < 0.01; *** p < 0.001. Image collected & cropped by CiteAb from the following publication (http://www.mdpi.com/1422-0067/16/1/439), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-3102024155459.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Involvement of CBP in BNIP3 expression regulated by HIF-1 alpha & FOXO3 under hypoxia. (A) UCB-hMSCs were incubated with hypoxia condition for 24 h. Co-immunoprecipitation of HIF-1 alpha & FOXO3 with IgG & CBP were shown in left panel. IgG was used as a negative control. The total protein expressions of HIF-1 alpha, FOXO3, CBP & beta-actin in lysate were shown in right panel. n = 3. (B) CBP (20 μM) was pretreated to UCB-hMSCs, & cells were incubated with hypoxia for 24 h. The BNIP3 mRNA expression level was analyzed by qPCR. n = 6. (C) BNIP3 & beta-actin protein expressions were analyzed by western blot. Data represent mean ± S.E. n = 4. (D, E) Sample DNA was immuno-precipitated with RNA polymerase, IgG, HIF-1 alpha & FOXO3 specific antibodies. CHIP (top panel) & lysate (bottom panel) samples were amplified with the primers of GAPDH & BNIP3 promoters. Quantitative CHIP data was analyzed by qPCR, & shown in the right panel. n = 4. Western blot data were normalized by beta-actin, & qPCR data were normalized by ACTB mRNA expression level. Quantitative data are presented as a mean ± S.E.M. All blot images are representative. *p < 0.05 versus control, #p < 0.05 versus hypoxia. Image collected & cropped by CiteAb from the following publication (https://linkinghub.elsevier.com/retrieve/pii/S2213231717303804), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-3102024168213.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Precision quantitative image analysis workflow for the characterization of functional ITH.(a) Representative areas with positive immunohistochemical (IHC) staining for HIF-1 alpha, HIF-2 alpha, phospho-mTOR S2448, phospho-S6RP S235/236 & Ki-67. Only nuclear HIF-1 alpha & HIF-2 alpha expression was included in the analysis. Scale bar, 100 μm. (b) Overview of the quantitative IHC analysis process using a representative image of a whole-slide scanned pT1M1 tumour after phospho-mTOR S2448 staining. The image was overlaid with a virtual grid consisting of 1 mm2 squares that defines the tumour regions to be analysed. Non-cancerous tissue was subtracted manually (dark grey) & a positive pixel count was performed on the corrected area. Positivity per square was expressed as percentage of positive pixels. If a square contained non-tumorous tissue, the positivity was normalized to the corrected square area. Computed values were depicted as a heat map. Coloured squares represent tumour, whereas open squares indicate completely non-tumorous areas. Squares defined as peripheral tumour zone are marked by a black line. Scale bar, 1 cm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27291893), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Immunohistochemistry" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-3102024165739.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - ROS overproduction mediated apoptosis induced by combined treatment of DDP & HIF-1 alpha in PC-3 xenografts & cell culture. (a) ROS monitored by DHE (red) & nuclei by DAPI (blue) staining in PCa xenografts (scale bars, 50 µm). (b) MDA formation of PCa xenografts was examined after various treatments (c,d). PC-3 cells were treated with DDP, si-HIF-1 alpha plasmid, or both, in the presence or absence of NAC (5 mM) or DHLA (0.25 mM) for 24 h. Total lysates (c) & culture media (d) were used to detect cellular H2O2 level. (e,f) Western analysis for HIF-1 alpha as well as cleaved caspase-3 & PARP in PC-3 cells following various treatments. Data were presented as mean ± SD of three independent experiments. *p " data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-31020241683926.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Glucose uptake & mitochondrial clustering following RT & CT. Glucose uptake (a,b) & mitochondrial clustering (c,d) was evaluated in A549 & A549RR cells before, 12 & 24 hours following radiation alone (RT) or combined radiation & YC-1 treatment. Immunoblotting was performed to verify HIF-1 alpha & PDK-1 inhibition (e–g). Sample immunoblots were cropped horizontally at indicated molecular weight. Uncropped exposures can be seen in supplemental Fig. 1. Significant differences (p " data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-3102024155454.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Regulation of OA development by HIF-1 alpha. Plain radiographs of the entire bodies at 8 weeks of age (A), & knee joints 8 weeks after OA surgery (B) of Cntl & cKO littermates. Scale bars, 10 mm & 1 mm, respectively. (C) Safranin O & TUNEL staining’s of Cntl & KO knee joints at 8 weeks after OA surgery (n = 9 & 11, respectively). Tamoxifen induction was performed at 7 weeks. Inset boxes in top images indicate the regions of high magnification Safranin O & TUNEL images. Inset boxes in middle images indicate the regions of immunofluorescence in (G). White dotted lines in TUNEL images indicate the cartilage area. Scale bars, 400 µm & 100 µm, respectively. (D) OARSI (Osteoarthritis Research Society International) score of OA development. Lines show the mean ± SD. *P " data-badges="" data-application="Immunocytochemistry/ Immunofluorescence" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-31020241683940.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - A beta facilitates HIF1 alpha synthesis & autophagy inhibition via mTOR activation. (A) SK-N-MC cells exposed to A beta (5 μM) for 0–48 h. HIF1 alpha & beta-actin expression analyzed by WB. n = 3. (B) Cells pretreated w/ NAC (1 mM) for 30 min prior to A beta treatment for 24 h. HIF1 alpha & beta-actin expression analyzed by WB. n = 3. (C,E) Cells incubated w/ rapamycin (10 nM) for 30 min prior to A beta treatment for 24 h. Phosphorylation of 4EBP1 (Thr 37/46) & 4EBP1, phosphorylation of p70S6K1 (Thr 389), HIF1 alpha & beta-actin analyzed by WB. n = 6. (D) Protein samples immunoprecipitated by eukaryotic translation initiation factor 4E (eIF4E) antibody-conjugated protein A/G agarose beads. Samples blotted w/ 4EBP1 & eIF4E-specific antibodies. n = 3. (F) Cells exposed to PF4708671 (10 μM) for 30 min prior to A beta treatment for 24 h. HIF1 alpha & beta-actin expression detected by WB. n = 6. (G) Cells exposed to cycloheximide (4 μM) for 30 min prior to A beta treatment for 24 h. HIF1 alpha & beta-actin expressions detected by WB. n = 6. (H) Cells pretreated w/ rapamycin (10 nM) for 30 min, incubated w/ A beta for 24 h & analyzed by WB w/ LC3, p62 & beta-actin specific antibodies. n = 3–6. (I) LC3 puncta visualized by confocal microscopy. Presented results merged images. Green & red fluorescents indicate LC3 & PI respectively. Scale bars, 50 μm (magnification × 600). (J) Cells pretreated w/ trehalose (10 μM) for 30 min prior to A beta treatment for 24 h. Cytotoxicity measured by MTT assay at an absorbance of 545 nm using a microplate reader. Data present the mean ± SE. n = 6. (K) Cell viability measured by trypan blue exclusion assay. Data presented as a mean ± SE. n = 6. Each blot image presented as representative image. *p < 0.05 vs. control, #p < 0.05 vs. A beta treatment. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28790888), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-31020241555218.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - TSA reduces HIF-1 alpha & VEGF expression & up-regulates the expression of PEDF.Real-time PCR (A) & Western blot assays (B) were performed on RPE cells treated with 0–0.5 μM TSA & 150 μM CoCl2. (A) Cells were treated with 0–0.5 μM TSA for 14 h & then co-treated with 150 μM CoCl2 for 6 h for the analysis of gene expression by real-time PCR. Changes in HIF-1 alpha mRNA levels were not statistically significant. CoCl2 causes a fourfold enhancement of VEGF mRNA expression; but at 0.5 μM TSA, the mRNA level of VEGF reduces to less than half of that in cells treated with CoCl2 only. TSA induces a statistically significant increase in the mRNA level of PEDF. (B) Cells were treated with 0–0.5 μM TSA for 18 h & then co-treated with 150 μM CoCl2 for 6 h for Western blot analysis. TSA reduces the CoCl2-induced HIF-1 alpha & VEGF protein levels by 4.3-fold & 5.7-fold, respectively, & up-regulates PEDF protein level by threefold. (C) Densitometry data for Western blot of HIF-1 alpha, VEGF & PEDF. (*: t test p" data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-31020241554541.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Effects of resveratrol on the expression of SIRT1, PGC-1 alpha, HIF-1 alpha & apoptosis proteins in vivo. (A) Activation of the SIRT1–PGC-1 alpha–HIF-1 alpha signaling pathway in PC–AKI associated with DN in rabbits. Representative western blot images of proteins in the rabbits with DN treated with saline (Cont), resveratrol alone (Res), iohexol (PC–AKI), & co-treatment with resveratrol & iohexol (Res+PC–AKI). (B–H) Relative densitometry analysis of the ratios of SIRT1–PGC-1 alpha–HIF-1 alpha signaling proteins to beta-actin were expressed as mean ± standard error. *P < 0.05 vs. Cont; #P < 0.05 vs. PC–AKI. Cont, control; Res, resveratrol; PC–AKI, post-contrast acute kidney injury; SIRT1, silent information regulator l; HIF-1 alpha, hypoxia-inducible transcription factor-1 alpha; DN, diabetic nephropathy; PGC-1 alpha, peroxisome proliferator-activated receptor gamma coactivator-1 alpha. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31402864), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-310202415541924.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - A beta facilitates HIF1 alpha synthesis & autophagy inhibition via mTOR activation. (A) SK-N-MC cells exposed to A beta (5 μM) for 0–48 h. HIF1 alpha & beta-actin expression analyzed by WB. n = 3. (B) Cells pretreated w/ NAC (1 mM) for 30 min prior to A beta treatment for 24 h. HIF1 alpha & beta-actin expression analyzed by WB. n = 3. (C,E) Cells incubated w/ rapamycin (10 nM) for 30 min prior to A beta treatment for 24 h. Phosphorylation of 4EBP1 (Thr 37/46) & 4EBP1, phosphorylation of p70S6K1 (Thr 389), HIF1 alpha & beta-actin analyzed by WB. n = 6. (D) Protein samples immunoprecipitated by eukaryotic translation initiation factor 4E (eIF4E) antibody-conjugated protein A/G agarose beads. Samples blotted w/ 4EBP1 & eIF4E-specific antibodies. n = 3. (F) Cells exposed to PF4708671 (10 μM) for 30 min prior to A beta treatment for 24 h. HIF1 alpha & beta-actin expression detected by WB. n = 6. (G) Cells exposed to cycloheximide (4 μM) for 30 min prior to A beta treatment for 24 h. HIF1 alpha & beta-actin expressions detected by WB. n = 6. (H) Cells pretreated w/ rapamycin (10 nM) for 30 min, incubated w/ A beta for 24 h & analyzed by WB w/ LC3, p62 & beta-actin specific antibodies. n = 3–6. (I) LC3 puncta visualized by confocal microscopy. Presented results merged images. Green & red fluorescents indicate LC3 & PI respectively. Scale bars, 50 μm (magnification × 600). (J) Cells pretreated w/ trehalose (10 μM) for 30 min prior to A beta treatment for 24 h. Cytotoxicity measured by MTT assay at an absorbance of 545 nm using a microplate reader. Data present the mean ± SE. n = 6. (K) Cell viability measured by trypan blue exclusion assay. Data presented as a mean ± SE. n = 6. Each blot image presented as representative image. *p < 0.05 vs. control, #p < 0.05 vs. A beta treatment. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28790888), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-3102024155458.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Role of HIF-1 alpha, FOXO3 in hypoxia-induced BNIP3 expression. (A) UCB-hMSCs were incubated with various durations of hypoxia (0–48 h). The protein expressions of HIF-1 alpha & beta-actin were detected by western blot. n = 4. (B) UCB-hMSCs were pretreated with NAC (5 mM) for 30 min prior to hypoxia incubation for 24 h. The protein expressions of HIF-1 alpha, lamin A/C & beta-tubulin in non-nuclear & nuclear fractionized cell samples were assessed by using western blot. n = 3. (C) UCB-hMSCs were immuno-stained with HIF-1 alpha & PI (magnification ×600). Scale bars, 37.5 µm. (D) HIF1A siRNA or NT siRNA was transfected to cells prior to hypoxia treatment for 24 h. The mRNA expression of BNIP3 was analyzed by qPCR. n = 6. (E) The protein expressions of BNIP3 & HIF-1 alpha were detected by western blot. n = 4. (F) NAC (5 mM) was pretreated to UCB-hMSCs prior to hypoxia treatment for 24 h. FOXO3, lamin A/C & beta-tubulin proteins expressions were assessed by western blot. n = 3. (G) FOXO3 siRNA transfected to UCB-hMSCs prior to hypoxia treatment for 24 h. The FOXO3 mRNA expression was measured by qPCR. n = 6. (H) BNIP3, FOXO3 & beta-actin expressions were detected by western blot. n = 3. Western blot data were normalized by beta-actin, & qPCR data were normalized by ACTB mRNA expression level. Lamin A/C & beta-tubulin were used as nuclear & non-nuclear protein controls, respectively. Quantitative data are presented as a mean ± S.E.M. All blots & confocal images are representative. *p < 0.05 versus control, #p < 0.05 versus hypoxia. Image collected & cropped by CiteAb from the following publication (https://linkinghub.elsevier.com/retrieve/pii/S2213231717303804), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-31020241682192.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - HIF1 alpha protein is suppressed by ED71 but not by 1,25(OH)2D3.(A) Western analysis of Raw264.7 cells cultured in hypoxic conditions with or without 10−7 M of ED71 or 1,25(OH)2D3 (1,25D). (B) Hif1 alpha mRNA levels in Raw264.7 cells cultured in hypoxic conditions were analyzed by realtime PCR in the presence or absence of 10−7 M ED71 or 1,25(OH)2D3. Data represent mean Hif1 alpha expression relative to that of Actb ± SD (n = 5). (C) Levels of VDR transcripts in Raw264.7 cells transfected with shRNA targeting the VDR (shVDR) or control shRNA (Control) were determined by realtime PCR. Data represent mean VDR expression relative to that of Actb ± SD (n = 5). (D) Western analysis of control (shControl) or VDR-suppressed (shVDR#1 or shVDR#2) Raw264.7 transformants cultured in hypoxic conditions with ED71 or 1,25(OH)2D3 (1,25D), both at 10−7 M. (E) M-CSF-dependent Ctsk Cre/Hifflox/flox cells were cultured in normoxic conditions to suppress HIF1 alpha in the presence of M-CSF (50 ng/ml) plus RANKL (25 ng/ml) with either ED71 or 1,25(OH)2D3 (1,25D) both at 10−7M for 4 days. Expression of Ctsk & NFATc1 was then assessed by realtime PCR. Data represent mean Ctsk or NFATc1 expression relative to that of Actb ± SD (n = 5). *P" data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-310202415523934.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Effect of PACAP, PACAP6-38, VIP, & VIP antagonist on expression of HIF-1 alpha, HIF-2 alpha, & EGFR in U87-MG cells under normoxia & hypoxia. (A) Representative immunoblots of HIF-1 alpha, HIF-2 alpha, & EGFR expression on U87MG cells grown normoxia or exposed to hypoxia. (B) The bar graphs show quantitative analysis of signals obtained by immunoblots resulting from three independent experiments. Relative band densities were quantified by using ImageJ software. Protein levels are expressed as arbitrary units obtained after normalization to beta-tubulin which was used as loading control. Data represent means ± SEM (*p < 0.05, **p < 0.01, or ***p < 0.001 vs. Vhl under normoxia; ###p < 0.001 vs. Vhl under hypoxia $$p < 0.01 or $$$p < 0.001 vs. PACAP, as determined by one-way ANOVA followed by the Tukey post hoc test). Image collected & cropped by CiteAb from the following publication (http://journal.frontiersin.org/Article/10.3389/fphar.2016.00139/abstract), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-310202415541934.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Lack of HIF-P4H-1 increases the amount of p53. (a) Western blot analysis of p53 in wt & Hif-p4h-1−/− MEFs cultured in 21% or 1% O2 for 24 h. (b,c) Western blot analysis of p53 in Hif-p4h-1 & scrambled (Scrm) siRNA transfected HEK293 cells (b) & wt MEFs treated with 50 µM FG4497 for 24 h (c). (d) qPCR analysis of p53 mRNA in wt & Hif-p4h-1−/− MEFs cultured in 21% or 1% O2 for 24 h. (e) Analysis of p53 protein turnover rate. Hif-p4h-1−/− & wt MEFs were treated with 200 μg/ml of cycloheximide for the indicated time points & whole cell lysates were blotted for p53. (f) Western blot analysis of MDM2 in wt & Hif-p4h-1−/− MEFs cultured in 21% or 1% O2 for 24 h. (g) Western blot analysis of p53 & MDM2 in wt MEFs treated with 10 μM nutlin-3a for 24 h with or without overexpression of human HIF-P4H-1 (OE). (h,i) Western blot analysis of HIF1 alpha in wt & Hif-p4h-1−/− MEFs cultured in 21% or 1% O2 for 24 h (h) & in scrambled & Hif-p4h-1 siRNA transfected HEK293 cells (i). (j) Western blot analysis of ubiquitination of p53 in Hif-p4h-1 & scrambled siRNA transfected HEK293 cells. The cells were transfected with HA-ubiquitin & endogenous p53 was immunoprecipitated followed by Western blotting with anti-HA & anti-P53 antibodies. (k) Western blot analysis of p53 in Hif-p4h-1−/− & wt MEFs were treated with or without 10 μM MG132 for 5 h. Data are presented as representative Western blots & as mean ± s.d., n = at least 3 individual MEF isolates or experiments. *P " data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-31020241682138.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - NDRG1 was regulated by HIF‐1/2 alpha. (A‐E) The protein expression of the shown genes was detected by Western blot, respectively; beta‐actin was used as the loading control. (A) RCC4 & 786‐O cells (B) were infected with VHL vector (VHL) or empty vector (EV). (C) Caki‐1 cells were transfected with VHL shRNAs (shVHL) or non‐specific control (NC). (D) RCC4 cells were infected with HIF‐1 alpha siRNAs (siHIF‐1 alpha) or non‐specific control (NC). (E) 786‐O cells were transfected with HIF‐2 alpha shRNAs (shHIF‐2 alpha) or non‐specific control (NC). (F) Representative images of HIF‐1 alpha, HIF‐2 alpha & NDRG1 protein expression from the IHC staining of human clear cell renal cell cancer specimens. Patient I: The protein levels of HIF‐1 alpha, HIF‐2 alpha & NDRG1 were low. Patient II: The protein levels of HIF‐1 alpha, HIF‐2 alpha & NDRG1 were medium. Patient III: The protein levels of HIF‐1 alpha, HIF‐2 alpha & NDRG1 were high (scale bar, 50 μm). (G & H) The protein level of NDRG1 is significantly correlated with HIF‐1 alpha (G) & HIF‐2 alpha (H). The protein levels were evaluated according to IHC scores, representing very low (score 0‐2), low (score 3‐4), high (score 5‐8) & strong (score 9‐12). The subjects were divided into four groups according to the IHC scores of HIFs in the tumours. (I) The protein level of NDRG1 is significantly correlated with the average of HIF‐1 alpha & HIF‐2 alpha in ccRCC tissues. The protein levels are evaluated according to IHC scores, representing very low (score " data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-31020241553517.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - In-vivo lentiviral delivery of Cre-recombinase to renal tubular epithelium results in recombination of target genes.(a) Diagram of pCCIE lentiviral construct. Cre, Cre-recombinase; IRES, internal ribosome entry site; GFP, Green fluorescent protein. (b) Anti-VHL, HIF1a & GAPDH immunoblots of renal cortical protein lysates from Vhlwt/wt & Vhlfl/fl mice intrarenally injected with CCIE. Samples were collected 12 months post infection with each column representing an individual mouse. Blots were cropped to improve clarity, full-length blots are presented in Supplementary Fig. S3a. (c) Histological images of renal sections from Vhlwt/wt & Vhlfl/fl mice intrarenally injected with CCIE at 12 months post injection (stains & antibodies as indicated). Scale bars, 100 μm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26046460), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-310202415532037.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - WNT11 is induced by hypoxia or hypoxic mimetics in different cell types.(A) Increased Wnt11 mRNA in EMSC adipocytes (Day 12) after hypoxia-mimetic treatments. EMSC adipocytes were treated with CoCl2 (0.1 mM), DFO (0.1 mM) or DMOG (0.1 mM) for 24 hrs. Values were normalized to Tbp mRNA & are expressed relative to control (n = 3). (B,C) Increased Wnt11 mRNA by hypoxia in EMSC preadipocytes & adipocytes (Day 0–12 after differentiation) (B), & C2C12 myoblast & myocyte (Day 0 & 8 after differentiation) (C). Wnt11 mRNA was assessed by quantitative PCR in cells exposed to air (21% O2) or hypoxia (1% O2) for 24 hrs. (n = 4). Values were normalized to Tbp mRNA & are expressed relative to 21% O2 samples (left panel). (D) Immunoblot analyses of HeLa cells under normal air or hypoxia for 24 hrs. (E,F) Induction of Wnt11 by increasing concentrations of DMOG in MDA-MB-231 cells (E) & 4T1 cells (F). (G) EMSCs treated with 0.1 mM DMOG for the indicated times. Wnt11 & Vegf mRNA expression was measured by qPCR & normalized to Tbp mRNA (n = 4). (H) WNT11 protein levels after DMOG treatment normalized to alpha-Tubulin (upper panel; n = 4). Representative immunoblots of EMSCs treated with 0.1 mM DMOG for the indicated times (Lower panel). (I) Protein expression in MDA-MB-231 cells treated with 0.1 mM DMOG. (J) Induction of Wnt11 promoter activity by hypoxia or hypoxia mimetics. pGL3-Wnt11 promoter plasmid was transfected into C2C12 cells. Cells were incubated with DMOG (left panel, n = 4) or under 21% O2 or 1% O2 (right panel, n = 8) for 24 hrs. For panels (A–C,G,H,J), values are mean ± s.e.m. *p " data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-31020241554512.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - A beta facilitates HIF1 alpha synthesis & autophagy inhibition via mTOR activation. (A) SK-N-MC cells exposed to A beta (5 μM) for 0–48 h. HIF1 alpha & beta-actin expression analyzed by WB. n = 3. (B) Cells pretreated w/ NAC (1 mM) for 30 min prior to A beta treatment for 24 h. HIF1 alpha & beta-actin expression analyzed by WB. n = 3. (C,E) Cells incubated w/ rapamycin (10 nM) for 30 min prior to A beta treatment for 24 h. Phosphorylation of 4EBP1 (Thr 37/46) & 4EBP1, phosphorylation of p70S6K1 (Thr 389), HIF1 alpha & beta-actin analyzed by WB. n = 6. (D) Protein samples immunoprecipitated by eukaryotic translation initiation factor 4E (eIF4E) antibody-conjugated protein A/G agarose beads. Samples blotted w/ 4EBP1 & eIF4E-specific antibodies. n = 3. (F) Cells exposed to PF4708671 (10 μM) for 30 min prior to A beta treatment for 24 h. HIF1 alpha & beta-actin expression detected by WB. n = 6. (G) Cells exposed to cycloheximide (4 μM) for 30 min prior to A beta treatment for 24 h. HIF1 alpha & beta-actin expressions detected by WB. n = 6. (H) Cells pretreated w/ rapamycin (10 nM) for 30 min, incubated w/ A beta for 24 h & analyzed by WB w/ LC3, p62 & beta-actin specific antibodies. n = 3–6. (I) LC3 puncta visualized by confocal microscopy. Presented results merged images. Green & red fluorescents indicate LC3 & PI respectively. Scale bars, 50 μm (magnification × 600). (J) Cells pretreated w/ trehalose (10 μM) for 30 min prior to A beta treatment for 24 h. Cytotoxicity measured by MTT assay at an absorbance of 545 nm using a microplate reader. Data present the mean ± SE. n = 6. (K) Cell viability measured by trypan blue exclusion assay. Data presented as a mean ± SE. n = 6. Each blot image presented as representative image. *p < 0.05 vs. control, #p < 0.05 vs. A beta treatment. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28790888), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-105_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-31020241555220.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] -" data-caption="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Induction of HIF-1 alpha & a glycolytic response by VLX600.(a) GSEA plots of hypoxia, glycolysis, mitosis & p53 networks based on the gene expression profiles of HCT116 MCS or HCT116HIF-1 alpha−/− MCS exposed for 6 h to 6 μM VLX600. NES, normalized enrichment score; P values refer to the nominal P values generated after 1,000 permutations. (b) Monolayer HCT116 cells were exposed to 6 μM VLX600 & analysed for HIF-1 alpha expression by western blotting after 6 h. (c) Spheroids were exposed to VLX600 for different times, sectioned & stained for HIF-1 alpha. The spheroid core areas were lack of HIF-1 alpha induction. Scale bar, 250 μm (d) HCT116HIF-1 alpha−/− MCS show strong positivity for active caspase-3 in core areas. Spheroids were fixed & stained for active caspase-3 5 days after formation; scale bar, 250 μm. (e) HCT116 & HCT116HIF-1 alpha−/− MCS were exposed for 72 h to VLX600 (6 μM) followed by drug wash-out. Diameters of the MCS were recorded & converted to volumes. Volume determinations are inaccurate at 4–10 days since MCS disintegrate & dead cells stick to spheroid structures (the transient increase in the volume of VLX600-treated HCT116HIF-1 alpha−/− MCS is likely to be an artifact). Shown are means±s.d. (n=3). (f) HCT116, HCT116HIF-1 alpha−/− or HCT116p53−/− monolayer cells were exposed to VLX600 & viability was determined after 72 h using the fluorometric microculture cytotoxicity assay. Shown are means±s.e.m. (n=3). (g) Monolayer HCT116 cells were exposed to 6 μM VLX600 & analysed for p53 expression by immunoblotting at the times indicated. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24548894), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-105" data-width="250" data-height="250" class="none"></i> </div> </div> </div> </div> <div class="atc-block-search atc-block-collapsible" data-type="search" data-blockid="ProductCart-NB100-105" > <form action="/commerce/addtocart" method="post" class="px-3 pt-3 add-to-cart-block" data-productcode="NB100-105"> <div class="commerce-atc-full-header atc-header col-four row border-bottom pb-1 m-0"> <div class="commerce-atc-catnum atc-head col-4 col-md-4 font-weight-bold">Catalog #</div> <div class="commerce-atc-availability atc-head col-md-3 font-weight-bold d-none d-md-block">Availability </div> <div class="commerce-atc-price atc-head col-5 col-md-3 font-weight-bold">Size / Price</div> <div class="commerce-atc-quantity atc-head col-3 col-md-2 text-right font-weight-bold">Qty</div> </div> <div class="spinner-border spinner-border-sm mt-3 ml-3" role="status"> <span class="sr-only">Loading...</span> </div> <div class="d-none search-product-mask"> <div id="ProductCart-NB100-105" class="atc-lines"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="NB100-105"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> NB100-105 </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="NB100-105SS"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> NB100-105SS </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> </div> <div class="atc-control ProductCart-NB100-105-control text-right"> <div class="row d-flex justify-content-end mx-0 p-0"> <div class="order-md-last m___button-atc mx-1"> <button type="submit" value="Add To Cart" class="atc-add-button btn a___button-atc m-1 col-xs-4 order-last btn-md-block w-100"> Add To Cart </button> </div> <div class="m___button-atc mx-1"> <a href="/services/bulk-quotes?product_code=NB100-105" class="atc-bulk-button btn a___button-secondary m-1 col-xs-4 btn-md-block w-100" rel="nofollow">Bulk Order</a> </div> </div> </div> </div> </form> </div> </div> </div> </div> <div class="search_result atc-container pb-3" data-brand="novus" data-category="Primary Antibodies" data-id="NB100-479" data-name="HIF-1 alpha Antibody - BSA Free" data-position="1"> <div class="search_details border bg-white p-3 rounded"> <div class="row"> <div class="col-md-9"> <a href="/p/antibodies/hif-1-alpha-antibody_nb100-479" title="HIF-1 alpha Antibody - BSA Free"> <h4>HIF-1 alpha Antibody - BSA Free </h4> </a> </div> <div class="search_result_brand_logo col-md-3 mb-1 d-flex justify-content-xl-end product-brand-logo"> <img alt="Novus Biologicals, a Bio-Techne brand" src="https://resources.bio-techne.com/bio-techne-assets/images/logos/bio-techne-novus-biologicals.svg" class="d-flex w-75"> </div> </div> <div class="row"> <div class="col-md-9"> <div class="mb-1 d-inline-flex"> <div class="align-middle my-1 mr-3 d-block d-md-inline"> Catalog #: <a href="/p/antibodies/hif-1-alpha-antibody_nb100-479" title="HIF-1 alpha Antibody - BSA Free"> NB100-479 </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold"> <a href="/p/antibodies/hif-1-alpha-antibody_nb100-479#reviews" class="searchImage_star4"> (29) </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold"> <a href="/p/antibodies/hif-1-alpha-antibody_nb100-479#citations"><span class="icon-icon-product-citations"></span>Citations (518)</a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold text-nowrap"> <a href="/p/antibodies/hif-1-alpha-antibody_nb100-479#product-documents"><span class="icon-icon-pdf text-error-red"></span> Product Documents</a> </div> <div class="d-none d-md-inline my-1"> <a href="#" class="search_result_images"><span class="icon-icon-image-camera-plus"></span> Images (86)</a> </div> </div> <div class="d-none d-md-block"> <span class="search_result_applications"></span><span class="applications_title"><strong>Applications:</strong></span> IHC, WB, ICC/IF, Flow, IP, B/N, ChIP, Func, KD, KO, Simple Western </div> <div class="d-none d-md-block"> <span class="species_title"><strong>Reactivity:</strong></span> Human, Mouse, Rat, Canine, Primate, +8 More </div> </div> <div class="col-md-3"> <button class="py-1 btn-default btn-block atc-view-more collapsed" data-toggle="collapse" href="#collapseButton-1" role="button" aria-expanded="false" aria-controls="collapseButton">View More</button> </div> </div> <div class="commerce-atc-search pt-3 collapse" id="collapseButton-1"> <div class="row"> <div class="search_result_description col-xs-12 col-md-9"> <table class="tablesaw tablesaw-stack mb-2"> <tbody> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Reactivity:</strong></td> <td class="pb-1 w-75 text-break"> Human, Mouse, Rat, Canine, Primate, +8 More </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Details:</strong></td> <td class="pb-1 w-75 text-break"> Rabbit IgG Polyclonal </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Applications:</strong></td> <td class="pb-1 w-75 text-break"> IHC, WB, ICC/IF, Flow, IP, +6 More </td> </tr> </tbody> </table> <div class="compare_tool_select form-inline mb-2" data-sku="nb100-479"></div> </div> <div class="search_result_image_wrapper col-xs-12 col-md-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="novus"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src=https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-Chromatin-Immunoprecipitation-NB100-479-img0022.jpg data-srcset=https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-Chromatin-Immunoprecipitation-NB100-479-img0022.jpg alt="ChIP-qPCR Analysis of HIF-1 alpha in Treated Human MDA-MB-231 Breast Cancer Cells" title="ChIP-qPCR Analysis of HIF-1 alpha in Treated Human MDA-MB-231 Breast Cancer Cells" data-caption="ChIP-qPCR assay of HIF-1 binding to hypoxia response element (HRE) of the PKM2 gene in human MDA-MB-231 breast cancer cells treated with vehicle or DMOG (HIF inducer) for 8 hours. Negative control: immunoprecipitation was performed with IgG. Image courtesy of Gregg Semenza, Johns Hopkins University School of Medicine." data-badges="<span class="biological-validation"></span>" data-application="Chromatin Immunoprecipitation" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> <span class="image-count-text align-middle">(86)</span> </div> </div> </div> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-Western-Blot-NB100-479-img0025.jpg" data-alt="Western Blot Detection of Nuclear HIF-1 alpha in Multiple Cell Types at the Time of Irradiation" title="Western Blot Detection of Nuclear HIF-1 alpha in Multiple Cell Types at the Time of Irradiation" data-caption="Normoxic pre-treatment sensitizes glioma cells to radiation after graded chronic hypoxic (GCH) exposure. The results of anchorage-independent colony forming assays are shown for U87, U87-luc, GL261 glioma cells and 0308 GSCs after 5 Gy radiation exposure under varying O2 conditions. For easier comparisons among cell types, raw values are expressed as a % of the cell type's negative (non-irradiated) control and the means & SEMs are plotted. Each result represents at least 3 independent samples, plated in triplicate. Holm-Sidak comparisons for multiple groups were used for statistical comparisons of raw values (*p" data-badges="<span class="biological-validation"></span>" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-Immunohistochemistry-NB100-479-img0030.jpg" data-alt="Immunohistological Analysis of HIF-1 alpha in Wounded and non-Wounded Mouse Tissues" title="Immunohistological Analysis of HIF-1 alpha in Wounded and non-Wounded Mouse Tissues" data-caption="Immunofluorescence of wounded tissue and normal unwounded skin. Representative images for immunofluorescent staining of HIF-1 alpha on healed wounded skin and adjacent normal unwounded skin of wild type, heterozygous K14-Cre/homozygous floxed PHD-2, and heterozygous Col1 alpha2-Cre-ER/homozygous floxed PHD-2 mice at 400x magnification. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0093373), licensed under a CC-BY license." data-badges="" data-application="Immunohistochemistry" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-Immunohistochemistry-Paraffin-NB100-479-img0013.jpg" data-alt="Staining of HIF-1 alpha in Paraffin Embedded Mouse Kidney with and without Blocking Peptide" title="Staining of HIF-1 alpha in Paraffin Embedded Mouse Kidney with and without Blocking Peptide" data-caption="Staining in normal mouse kidney showing (A) staining with HIF-1 alpha antibody and (B) blocking peptide used at 10-fold excess concentration. Images provided by Dr Yves Heremans. Please see the product review of NBC1-18422 for additional details." data-badges="" data-application="Immunohistochemistry-Paraffin" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-Western-Blot-NB100-479-img0018.jpg" data-alt="Detection of HIF-1 alpha in Mouse Hepatocytes in Western Blot" title="Detection of HIF-1 alpha in Mouse Hepatocytes in Western Blot" data-caption="Analysis of HIF-1 alpha in mouse hepatocytes (left lane: normoxic and right lane: hypoxic) using HIF-1 alpha antibody. Western blot image submitted by a verified customer review." data-badges="<span class="biological-validation"></span>" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-Immunohistochemistry-Paraffin-NB100-479-img0012.jpg" data-alt="Immunohistological Staining of HIF-1 alpha in Paraffin Embedded Mouse Skin Wound" title="Immunohistological Staining of HIF-1 alpha in Paraffin Embedded Mouse Skin Wound" data-caption="Analysis in paraffin section of mouse skin wound using NB100-479 at 1:6000. Image courtesy of product review by Ian Darby of RMIT University." data-badges="" data-application="Immunohistochemistry-Paraffin" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NB100-479-img0027.jpg" data-alt="Immunofluorescence Labeling and Confocal Microscopy Analysis of the Distribution of HIF-1 alpha and Abeta in APPsw Cells" title="Immunofluorescence Labeling and Confocal Microscopy Analysis of the Distribution of HIF-1 alpha and Abeta in APPsw Cells" data-caption="Immunofluorescence (IF) labeling and confocal microscopy analysis of the distribution of HIF-1alpha (A1-D1) and Abeta (A2-D2) in APPsw cells. DFO treatment significantly increased HIF-1alpha IF (D1) and reduced the Abeta IF (D2) in APPsw cells compared with controls (A1,A2). (A3-D3) DAPI was used to detect the nucleus (blue). (A4-D4) Merged images from the double channels indicated that DFO has an inhibitory effect on Abeta formation, and is involved in HIF-1alpha stabilization in APPsw cells. Fe treatment (100 uM) decreased HIF-1alpha (B1) and increased the Abeta levels (B2). DFO treatment reversed the reduction of HIF-1alpha (C1) and the increase of Abeta (C2) in cultures pretreated with 100 uM Fe. Scale bar = 20 um. Image collected and cropped by CiteAb from the following publication (https://journal.frontiersin.org/Article/10.3389/fnagi.2015.00104/abstract) licensed under a CC-BY license." data-badges="<span class="biological-validation"></span>" data-application="Immunocytochemistry/ Immunofluorescence" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-Chromatin-Immunoprecipitation-NB100-479-img0029.jpg" data-alt="Chromatin Immunoprecipitation Using HIF-1 alpha Antibody in MDA-MB-231 Cells" title="Chromatin Immunoprecipitation Using HIF-1 alpha Antibody in MDA-MB-231 Cells" data-caption="MDA-MB-231 cells were exposed to 20% or 1% O2 for 16 hours, and chromatin immunoprecipitation (ChIP) was performed with the indicated antibody (Ab). Primers flanking the HIF binding site in the S100A10 gene were used for qPCR. ChIP image submitted by a verified customer review." data-badges="" data-application="Chromatin Immunoprecipitation" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-Western-Blot-NB100-479-img0016.jpg" data-alt="Western Blot Analysis of HIF-1 alpha in PC12 Cells at 20% and 1% Oxygen" title="Western Blot Analysis of HIF-1 alpha in PC12 Cells at 20% and 1% Oxygen" data-caption="Analysis in A. PC12 (rat) cells, 20% oxygen and B. PC12 (rat) cells, 1% oxygen." data-badges="<span class="biological-validation"></span>" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-Western-Blot-NB100-479-img0017.jpg" data-alt="Western Blotting of HIF-1 alpha in Treated and Untreated Cos-7 Nuclear Extract" title="Western Blotting of HIF-1 alpha in Treated and Untreated Cos-7 Nuclear Extract" data-caption="Analysis in a hypoxic sample. Lane 1: CoCl2 treated Cos-7 nuclear extract (50 ug, hypoxic). Lane 2: Untreated Cos-7 nuclear extract (50 ug, normoxic). 10 second ECL exposure." data-badges="<span class="biological-validation"></span>" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-Western-Blot-NB100-479-img0020.jpg" data-alt="Detection of HIF-1 alpha in U251 Cells by Western Blot" title="Detection of HIF-1 alpha in U251 Cells by Western Blot" data-caption="U251 cells exposed to 4 hour hypoxia (0.5% oxygen) incubation. Western blot image submitted by a verified customer review." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NB100-479-img0011.jpg" data-alt="Immunocytochemistry/Immunofluorescence Staining of HIF-1 alpha in RCC4 Cells" title="Immunocytochemistry/Immunofluorescence Staining of HIF-1 alpha in RCC4 Cells" data-caption="Detection of HIF-1 Alpha (green) in RCC4 cells using NB100-479. Nuclei (Blue) were counterstained using Hoechst 33258." data-badges="" data-application="Immunocytochemistry/ Immunofluorescence" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-Immunohistochemistry-Paraffin-NB100-479-img0015.jpg" data-alt="Immunohistological Staining of HIF-1 alpha in Paraffin Embedded Human Kidney Tumor" title="Immunohistological Staining of HIF-1 alpha in Paraffin Embedded Human Kidney Tumor" data-caption="Analysis of HIF-1 alpha in human kidney tumor." data-badges="" data-application="Immunohistochemistry-Paraffin" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-Immunohistochemistry-NB100-479-img0019.jpg" data-alt="Immunohistological Staining of HIF-1 alpha in Human Placenta" title="Immunohistological Staining of HIF-1 alpha in Human Placenta" data-caption="Staining of human placenta, villi." data-badges="" data-application="Immunohistochemistry" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-Immunohistochemistry-Paraffin-NB100-479-img0028.jpg" data-alt="Staining of HIF-1 alpha in i.v. CDDP and i.a. CDDP Administered Tumor Tissue" title="Staining of HIF-1 alpha in i.v. CDDP and i.a. CDDP Administered Tumor Tissue" data-caption="Effects of different routes of CDDP administration on remaining viable tumor tissue. Representative images of tumor tissue (i.v. CDDP, i.a. CDDP) stained for HIF-1alpha (20X). Scale bar corresponds to 100 um. Image collected and cropped by CiteAb from the following publication (https://jeccr.biomedcentral.com/articles/10.1186/s13046-016-0392-1), licensed under a CC-BY license." data-badges="" data-application="Immunohistochemistry-Paraffin" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-Immunohistochemistry-NB100-479-img0026.jpg" data-alt="Immunohistological Imaging of HIF-1 alpha in Preimplantation Embryos" title="Immunohistological Imaging of HIF-1 alpha in Preimplantation Embryos" data-caption="Hif1alpha expression in preimplantation embryos. Co-staining of OCT4 and Hif1alpha in inner cell mass of blastocyst (top) and negative control images without primary antibodies (bottom). Scale bar: 30um. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0154309) licensed under a CC-BY license." data-badges="" data-application="Immunohistochemistry" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-Simple-Western-NB100-479-img0032.jpg" data-alt="Simple Western Analysis of HIF-1 alpha in MSCs" title="Simple Western Analysis of HIF-1 alpha in MSCs" data-caption="Simple Western lane view shows lysates of MCF +/- DMOG, BioSpherix MSCs in hypoxic conditions, and BioSpherix MSCs in normoxic conditions loaded at 0.5 mg/ml. A band was detected for HIF-1 alpha at approximately 116 kDa (as indicated) using NB100-479 (1:100 dilution) followed by Anti-Rabbit Secondary Antibody (042-206, ProteinSimple). This experiment was conducted under standard assay conditions, and using the 12-230 kDa separation module (SW-W004). Non-specific interaction with the 230 kDa Simple Western standard seen with this antibody. Image from an internal validation." data-badges="" data-application="Simple Western" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-479_rabbit-polyclonal-hif-1-alpha-antibody-271220231253358.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] -" data-caption="Cofilin phosphorylation is HIF-1 alpha dependent.Wt, shC, and the HIF-1 alpha knock down cell clones c1 and c2 cells were lysed after 24 hrs of normoxia (20% O2) or hypoxia (1% O2). Cell extracts were analysed by Western blots. Note that p-cofilin levels are reduced in c2 and c2 cells compared to wt and shC cells at 20% O2 and 1% O2." data-badges="<span class="knockout-validation"></span>" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-479_rabbit-polyclonal-hif-1-alpha-antibody-310202415304234.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] -" data-caption="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] - Hypoxic markers accumulate in miR-29-sponge Zebrafish hearts. (A) Representative western blot analysis of hypoxia inducible factor 1 alpha (HIF1 alpha) expression in Wild Type & miR-29-sponge Zebrafish heart. In each condition, alpha-tubulin was used as loading control. Three independent experiments were performed. Full-length blot is presented in Supplementary Figure 6.(B) qRT-PCR mRNA analysis of hypoxia associated genes: erythropoietin alpha (epoa); hexokinase2 (hk2); heme oxygenase1a (hmox1a); lactate dehydrogenase A (ldha); cyclin-dependent kinase inhibitor 1B (p27) in Wild Type (black circles; n = 4) & miR-29-sponge (gray squares; n = 4) Zebrafish hearts expressed as fold-change versus Wild Type samples. *p " data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-479_rabbit-polyclonal-hif-1-alpha-antibody-310202415291977.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody - BSA Free [NB100-479] -" data-caption="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody - BSA Free [NB100-479] - (a,b) Immunofluorescence staining of (a) HIF-1 alpha (green), the macrophage marker F4/80 (red) & DAPI nuclear stain (blue), & (b) lactate dehydrogenase (LDH, green), F4/80 (red) & DAPI (blue) in WAT of lean & obese mice. Colocalization is shown in the merged image (arrows). Scale bar = 100 µm. (c,d) Relative mRNA expression of (c) Hif1a & Hif2a, & the HIF target genes (d) Vegfa & Glut1 in ATM isolated from lean & obese mice (n = 3). (e) Levels of succinate in ATM of lean & obese mice (n = 6). (f) Relative mRNA levels of the glutamate transporter Slc3a2 in ATM from lean & obese (n = 3). Data is expressed as mean ± s.e.m. *p " data-badges="" data-application="Immunocytochemistry/ Immunofluorescence" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-479_rabbit-polyclonal-hif-1-alpha-antibody-31020241530424.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] -" data-caption="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] - Increased expression of HIF alpha and/or Twist in A549 & H441 cells induced by the inhibition of PIMT & Thapsigargin(A) Immunoblotting of Slug, ZEB1, Snail1, Twist, & HIF1 alpha in A549 sh-PIMT & sh-control cells. (B, C) Immunoblotting & relative intensity of HIF1 alpha in A549 cells treated with Tg. (D) Immunoblotting of Slug, ZEB1, Snail1, Twist, & HIF1 alpha in si-control cells & si-PIMT H441 cells. (E, F) Immunoblotting & relative intensity of HIF1 alpha in H441 cells treated with Tg. #1 & #2 indicates si-RNA of J-010000-05-0002 & J-010000-07-0002, respectively. Image collected & cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.24324), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-479_rabbit-polyclonal-hif-1-alpha-antibody-31020241539457.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody - BSA Free [NB100-479] -" data-caption="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody - BSA Free [NB100-479] - Histology & immunofluorescence in rat corneal sections. a Hematoxylin & Eosin (H&E), b VEGF-A (green); c CCL2 (green); d TNF-alpha (green); e CXCL5 (green); f CD45 (green) & g HIF-1 alpha (green) staining in rat cornea tissue. Nuclear counterstaining by DAPI (blue) in fluorescent images Image collected & cropped by CiteAb from the following publication (http://link.springer.com/10.1007/s10456-018-9594-9), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Immunocytochemistry/ Immunofluorescence" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-479_rabbit-polyclonal-hif-1-alpha-antibody-31020241539597.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] -" data-caption="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] - Increased expression of HIF alpha and/or Twist in A549 & H441 cells induced by the inhibition of PIMT & Thapsigargin(A) Immunoblotting of Slug, ZEB1, Snail1, Twist, & HIF1 alpha in A549 sh-PIMT & sh-control cells. (B, C) Immunoblotting & relative intensity of HIF1 alpha in A549 cells treated with Tg. (D) Immunoblotting of Slug, ZEB1, Snail1, Twist, & HIF1 alpha in si-control cells & si-PIMT H441 cells. (E, F) Immunoblotting & relative intensity of HIF1 alpha in H441 cells treated with Tg. #1 & #2 indicates si-RNA of J-010000-05-0002 & J-010000-07-0002, respectively. Image collected & cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.24324), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-479_rabbit-polyclonal-hif-1-alpha-antibody-310202415394541.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Immunohistochemistry: HIF-1 alpha Antibody - BSA Free [NB100-479] -" data-caption="Immunohistochemistry: HIF-1 alpha Antibody - BSA Free [NB100-479] - White-jaded spleen & muscle in another NOD/SCID mouse model. One-week-old NOD/SCID mice were i.p. infected with EV-A71, & scarified after disease manifestation. aUpper panel: An atrophic & discolored spleen was identified in EV-A71-infected mice. Viral protein (VP1) & HIF1A were both expressed in the infected spleen. Lower panel: A saline control showed a normal-sized spleen with no VP1 & less expression of HIF1A. b Both HIF1A & VEGFA were strongly expressed in the whitened muscles infected with EV-A71, but not detectable in the saline control Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31711481), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Immunohistochemistry" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-479_rabbit-polyclonal-hif-1-alpha-antibody-310202415371998.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] -" data-caption="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] - The Neutralizing antibody against nucleolin eliminate HDGF-stimulated AKT/HIF-1 alpha/NF-kappa B/VEGF signaling in SCC4 oral cancer cells. a-d SCC4 cells were treated with recombinant HDGF protein (100 ng/ml) in the presence of anti-NCL or anti-IgG antibody (5 μg/ml) for 24 h before total protein extraction. Cell lysates were subjected to Western blotting with the indicated antibodies. beta-actin was used as an internal control for loading & transfer. Data were mean of three experiments. *, P " data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-479_rabbit-polyclonal-hif-1-alpha-antibody-31020241535695.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody - BSA Free [NB100-479] -" data-caption="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody - BSA Free [NB100-479] - Hif1 alpha expression in neonatal & adult ovary.(A) Section of P5 ovary showing MVH expression in all oocytes & Hif1 alpha expression only in small oocytes (primary follicles; top). Negative control images without primary antibodies (bottom). Scale bar: 50μm. (B) Section of adult ovary showing absence of Hif1 alpha expression in primary follicles detected with MVH staining (top). Negative control without primary antibody (bottom). Scale bar: 50μm. (C) Section of adult ovary showing Hif1 alpha expression in mature primary oocyte (arrow). Negative control without primary antibody (bottom). Scale bar: 100μm. (D) Image of a Metaphase II oocyte from superovulated three-month-old female showing Hif1 alpha expression. Scale bar: 50μm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27148974), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Immunocytochemistry/ Immunofluorescence" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-479_rabbit-polyclonal-hif-1-alpha-antibody-310202415345325.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] -" data-caption="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] - Effect of alpha-MSH on HIF-1 alpha expression & activity in B16-F10 melanoma cells during hypoxia.a, b alpha-MSH increased HIF-1 alpha mRNA & protein expression levels during hypoxia. c Cells were transfected with luciferase reporter driven by a promoter region containing HIF-1 alpha-specific binding sites before alpha-MSH treatment at the indicated time & concentration. The luciferase activities were measured immediately & expressed as means ± SD from triplicate experiments. d Relative mRNA expression levels were analyzed by real-time PCR. Data are expressed as fold change compared with control (means ± SD of triplicate experiments). *p " data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-479_rabbit-polyclonal-hif-1-alpha-antibody-31020241529197.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] -" data-caption="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] - Increased expression of HIF alpha and/or Twist in A549 & H441 cells induced by the inhibition of PIMT & Thapsigargin(A) Immunoblotting of Slug, ZEB1, Snail1, Twist, & HIF1 alpha in A549 sh-PIMT & sh-control cells. (B, C) Immunoblotting & relative intensity of HIF1 alpha in A549 cells treated with Tg. (D) Immunoblotting of Slug, ZEB1, Snail1, Twist, & HIF1 alpha in si-control cells & si-PIMT H441 cells. (E, F) Immunoblotting & relative intensity of HIF1 alpha in H441 cells treated with Tg. #1 & #2 indicates si-RNA of J-010000-05-0002 & J-010000-07-0002, respectively. Image collected & cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.24324), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-479_rabbit-polyclonal-hif-1-alpha-antibody-310202415304264.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody - BSA Free [NB100-479] -" data-caption="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody - BSA Free [NB100-479] - (a) Immunofluorescence staining for HIF-1 alpha (red), hypoxia probe pimonidazole (green) & DAPI nuclei (blue) in WAT of obese mice. Boxed region in the merged image shows HIF-1 alpha-positive cells in crown-like structures that are Pimo− (arrows). (b) HIF-1 alpha protein levels in BMDM treated with BSA (control) or BSA-conjugated palmitate (Palm) for 6, 12 & 24 hours. Tubulin is shown as an internal loading control. (c) Fold change in mRNA expression of Hif1a, Vegfa & Il1b in BMDM treated with palmitate or BSA. (d) Relative mRNA expression in BMDM treated as indicated in the presence or absence of a HIF-1 alpha inhibitor. Data are representative of 3 independent experiments & are expressed as mean ± s.e.m. *p " data-badges="" data-application="Immunocytochemistry/ Immunofluorescence" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-479_rabbit-polyclonal-hif-1-alpha-antibody-310202415371922.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] -" data-caption="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] - Hif1 alpha mediates Glut1 induction downstream of Bmp-mTORC1 signaling. a–c Western blot (a, c) or RT-qPCR (b) analyses in primary chondrocytes after 24 h of BMP2 treatment with or without mTOR inhibitors. Rapa: rapamycin. d Western blot analyses in primary chondrocytes in response to BMP2 with or without the Hif1 alpha inhibitors for 24 h. Quantification of all western blots denotes average fold change over vehicle control after normalization to beta-actin (±SD, n = 3). *p " data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-479_rabbit-polyclonal-hif-1-alpha-antibody-31020241539258.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] -" data-caption="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] - Tamoxifen administration-induced PHD2 deletion in skeletal muscle in Phd2f/f/Rosa26CreERT2 mice. a. PHD1, 2, & 3 deletion efficiency in the gastrocnemius muscles of tamoxifen-treated Phd2f/f/Rosa26CreERT2 mice was determined using qRT-PCR. Relative gene expression was determined using gastrocnemius muscle tissue cDNA (n = 3–4 mice per group). b. Anti-PHD2 & PHD3 Western blotting of gastrocnemius & soleus muscles at 6 weeks after tamoxifen administration. c. The expression of HIF-1 alpha in gastrocnemius & soleus at 5 weeks after tamoxifen administration. d. The level of hif-1 alpha mRNA in gastrocnemius at 5 weeks after tamoxifen administration. *p " data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-479_rabbit-polyclonal-hif-1-alpha-antibody-310202415304240.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody - BSA Free [NB100-479] -" data-caption="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody - BSA Free [NB100-479] - Hif1 alpha expression in neonatal & adult ovary.(A) Section of P5 ovary showing MVH expression in all oocytes & Hif1 alpha expression only in small oocytes (primary follicles; top). Negative control images without primary antibodies (bottom). Scale bar: 50μm. (B) Section of adult ovary showing absence of Hif1 alpha expression in primary follicles detected with MVH staining (top). Negative control without primary antibody (bottom). Scale bar: 50μm. (C) Section of adult ovary showing Hif1 alpha expression in mature primary oocyte (arrow). Negative control without primary antibody (bottom). Scale bar: 100μm. (D) Image of a Metaphase II oocyte from superovulated three-month-old female showing Hif1 alpha expression. Scale bar: 50μm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27148974), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Immunocytochemistry/ Immunofluorescence" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-479_rabbit-polyclonal-hif-1-alpha-antibody-310202415371924.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] -" data-caption="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] - FASN Regulates HIF-alpha Protein Stability & HIF Target Gene Expression. (A) FASN siRNA downregulates HIF-alpha protein levels. PREC, HeLa & HCT116 cells treated with FASN (+) or control (−) siRNA & harvested after 72 h. Cells exposed to hypoxia (1% oxygen) or maintained in normoxia (21% oxygen) 4 h before harvesting & subjecting WCEs to WB analysis. Unmodified (250 kDa) & modified (larger) FASN bands indicated by large arrowheads, REDD1 by a small arrowhead, & HIF-1 alpha & HIF-2 alpha by arrows. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28775317), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-479_rabbit-polyclonal-hif-1-alpha-antibody-31020241534537.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody - BSA Free [NB100-479] -" data-caption="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody - BSA Free [NB100-479] - Hif1 alpha expression in neonatal & adult ovary.(A) Section of P5 ovary showing MVH expression in all oocytes & Hif1 alpha expression only in small oocytes (primary follicles; top). Negative control images without primary antibodies (bottom). Scale bar: 50μm. (B) Section of adult ovary showing absence of Hif1 alpha expression in primary follicles detected with MVH staining (top). Negative control without primary antibody (bottom). Scale bar: 50μm. (C) Section of adult ovary showing Hif1 alpha expression in mature primary oocyte (arrow). Negative control without primary antibody (bottom). Scale bar: 100μm. (D) Image of a Metaphase II oocyte from superovulated three-month-old female showing Hif1 alpha expression. Scale bar: 50μm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27148974), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Immunocytochemistry/ Immunofluorescence" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-479_rabbit-polyclonal-hif-1-alpha-antibody-31020241535617.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] -" data-caption="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] - FASN Regulates HIF-alpha Protein Stability & HIF Target Gene Expression. (B) Downregulation of HIF-1 alpha & HIF-2 alpha in HCT116 cell clones carrying the FASN gene mutagenized using CRISPR-Cas9 (See Supplementary Fig. S3 for genomic DNA information). Protein samples prepared in normoxia (left column) or hypoxia (right column) for WB analysis. Asterisk indicates a non-specific band. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28775317), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-479_rabbit-polyclonal-hif-1-alpha-antibody-310202415371930.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] -" data-caption="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] - Effect of silencing Cx43 on the expression of HIF-1 alpha in astrocytes.Astrocytes were transfected with NT-siRNA or with Cx43-siRNA. At the indicated times the expression of HIF-1 alpha & Cx43 was analysed by Western blot. A) Representative Western blot of HIF-1 alpha, Cx43 & GAPDH showing that the decrease in Cx43 expression was concomitant with HIF-1 alpha up-regulation. B) HIF-1 alpha quantification. C) Cx43 quantification. The results are expressed as percentages of the level found in the NT-siRNA condition at time 0. ***p" data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-479_rabbit-polyclonal-hif-1-alpha-antibody-310202415392531.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] -" data-caption="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] - HDGF triggered AKT/HIF-1 alpha/NF-kappa B signaling in SCC4 oral cancer cells. a-d Cells were treated with recombinant HDGF (1–100 ng/ml) for 24 h & then harvested for total protein extraction. The cell lysates were separated by SDS-PAGE & detected by Western blotting with the indicated primary antibodies. beta-actin was used as an internal control for loading & transfer. Data were mean of three experiments. *, P " data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-479_rabbit-polyclonal-hif-1-alpha-antibody-31020241534353.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] -" data-caption="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] - Cofilin phosphorylation is HIF-1 alpha dependent.Wt, shC, & the HIF-1 alpha knock down cell clones c1 & c2 cells were lysed after 24 hrs of normoxia (20% O2) or hypoxia (1% O2). Cell extracts were analysed by Western blots. Note that p-cofilin levels are reduced in c2 & c2 cells compared to wt & shC cells at 20% O2 & 1% O2. Image collected & cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0069128), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-479_rabbit-polyclonal-hif-1-alpha-antibody-31020241539711.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] -" data-caption="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] - Genotyping of transgenes.PCR genotyping was performed for all four transgenes as described in materials & methods. Sizes of the amplified products obtained are: 240 bp for Hif-1 alpha (wild type); 274 bp for Hif-1 alphaflox/flox; 410 bp for Hif-2 alpha (wild type); 444bp for Hif-2 alphaflox/flox; 370 bpfor Cre transgene; 350 bpfor rtTA transgene. One representative sample was genotyped for the four transgenes from each of three generated mouse strains: SPC-rtTA-/tg/(tetO)7-Cre-/tg/Hif-1 alphafl/fl (Lane 1), SPC-rtTA-/tg/(tetO)7-Cre-/tg/Hif-2 alphafl/fl mouse (Lane 2), & SPC-rtTA-/tg/(tetO)7-Cre-/tg/Hif-1 alpha/2 alphafl/fl (Lane 3)). Image collected & cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0139270), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-479_rabbit-polyclonal-hif-1-alpha-antibody-310202415175294.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] -" data-caption="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] - Effect of 24 h UO on renal HIF-1 alpha/2 alpha expression.WT mice were exposed to either sham-operation or left UO, which continued for 24 hours, & then was released. The left kidneys were harvested immediately (d0), 2, 4 or 7 days after release of obstruction (n = 4 at each time point). Immunoblot analyses of HIF-1 alpha & HIF-2 alpha in left kidneys were then performed & co-detection of TBP was performed to assess equal loading. HIF protein bands were quantified & normalized to TBP. Data were expressed as mean ± SD, & the mean value obtained from non-operated control mice was arbitrarily defined as 1. *, p" data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-479_rabbit-polyclonal-hif-1-alpha-antibody-310202415384126.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody - BSA Free [NB100-479] -" data-caption="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody - BSA Free [NB100-479] - Hif1 alpha expression in 15.5dpc germ cells.(A) Sections of male (top) & female (bottom) gonads from 15.5 dpc Oct4-GFP embryos showing Oct4-GFP & Hif1 alpha expressions in germ cells. Scale bars: 50μm (male) & 20μm (female). (B) FACS-sorted male (top) & female (bottom) 15.5dpc germ cells showing Oct4-GFP & Hif1 alpha expressions. Scale bars: 100μm (male) & 50μm (female). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27148974), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Immunocytochemistry/ Immunofluorescence" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-479_rabbit-polyclonal-hif-1-alpha-antibody-3102024165722.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] -" data-caption="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] - Effect of ET-1 on glucose uptake in HIF-1 alpha-silenced astrocytes.Astrocytes were transfected with NT-siRNA or with HIF-1 alpha-siRNA. After 48 h, astrocytes were incubated in the absence (control, C) or presence of 0.1 µM ET-1 for 24 h. A) HIF-1 alpha, Hx-1, GLUT-3, Hx-2, GLUT-1 & GAPDH Western blots & quantification. The results are the means ± SEM of at least three independent experiments & they are expressed as percentages of the level found in the control NT-siRNA. B) Glucose uptake expressed as pmol of 2-deoxyglucose (2-DG) taken up per hour & per milligram of protein. The results show that the down-regulation of HIF-1 alpha levels promoted by HIF-1 alpha-siRNA decreased the rate of glucose uptake & the expression of GLUT-1, GLUT-3, Hx-1 & Hx-2, both in the control & in the ET-1 treated astrocytes. ***p" data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-479_rabbit-polyclonal-hif-1-alpha-antibody-31020241553511.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] -" data-caption="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] - Effect of ET-1 & Cx43 on HIF-1 alpha expression & glucose uptake when c-Src is inhibited.Astrocytes were preincubated with 100 ng/µL PP2 (c-Src inhibitor) or 100 ng/µL PP3 (inactive analogue) for 1 h. Then, cells were incubated in the absence (control) or presence of 0.1 µM ET-1 for 24 h. A) HIF-1 alpha Western blot & quantification. The results are expressed as percentages of the level found in the controls treated with PP3 & they show that the inhibitor of c-Src PP2 prevented the up-regulation of HIF-1 alpha promoted by ET-1. ***p" data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-479_rabbit-polyclonal-hif-1-alpha-antibody-3102024161266.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] -" data-caption="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] - Hypoxia reduces inflammatory processes & induces autophagy in Il-10−/− mice. a, b, c, d, e, f & g WT, Nrlp3−/−,Il-10−/−, & Il-10−/−Nrlp3−/−double knockout mice were subjected to normoxia (N, 21% O2) or hypoxia (H, 8% O2). After 18 h, mice were killed & colon biopsies were collected. Statistical analysis was performed using one-way ANOVA followed by Tukey’s post-test. Results represent mean + s.e.m., WT mice under normoxia: n = 5, WT mice under hypoxia: n = 5; Nrlp3−/− mice under normoxia: n = 4; Nrlp3−/− mice under hypoxia: n = 5; Il-10−/− mice under hypoxia: n = 4; Il-10−/− mice under hypoxia: n = 4; Il-10−/−Nrlp3−/− mice under normoxia: n = 7; Il-10−/−Nrlp3−/− mice under hypoxia: n = 9, *P " data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-479_rabbit-polyclonal-hif-1-alpha-antibody-3102024155522.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] -" data-caption="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] - Hypoxia reduces inflammatory processes & induces autophagy in the DSS mouse model of colitis. a, b, c, d, e, f & g WT & Nrlp3−/− mice were administered with DSS or DSS-free water & subjected to normoxia (N, 21% O2) or hypoxia (H, 8% O2). After 18 h, mice were killed & colon biopsies were collected. Statistical analysis was performed using one-way ANOVA followed by Tukey’s post-test. Results represent mean + s.e.m., WT mice under normoxia: n = 5, WT mice under normoxia: n = 5, DSS-treated WT mice under normoxia: n = 6; Nrlp3−/− mice under normoxia: n = 5; DSS-treated Nrlp3−/− mice under normoxia: n = 6; WT mice under hypoxia: n = 4, DSS-treated WT mice under hypoxia: n = 5; Nrlp3−/− mice under hypoxia: n = 3; DSS-treated Nrlp3−/− mice under hypoxia: n = 3, *P " data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-479_rabbit-polyclonal-hif-1-alpha-antibody-310202415535187.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] -" data-caption="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] - Effect of HIF-1 alpha silencing on alpha-MSH-induced apoptosis in B16-F10 melanoma cells during hypoxia.a Cells were transfected with shLacZ or shHIF-1 alpha plasmids for 48 h before harvest. HIF-1 alpha shRNA reduced the basal HIF-1 alpha mRNA & protein expression levels. b, c Relative mRNA expression levels were analyzed by real-time PCR. Data are expressed as fold change compared with control (means ± SD of triplicate experiments). d Cell lysates were analyzed by immunoblot using the indicated antibodies. beta-Actin was used as an internal control for loading & transfer. e The population of apoptotic cells was analyzed by flow cytometry & qualified as mean ± SD from triplicate experiments. *p " data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-479_rabbit-polyclonal-hif-1-alpha-antibody-31020241554526.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] -" data-caption="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] - FASN & CAD Binding Specificity of pVHL Mutants Derived from VHL Patients. (A) Schematic representation of pVHL domains. Dotted lines indicate the approximate positions of the HIF-1 alpha & Elongin C binding domains. The alpha & beta structural domains are also indicated. (B) Effects of pVHL mutations on interactions with E3 ligase components, HIF-alpha & other interacting proteins including FASN. pVHL mutants were stably expressed by lentiviral vectors in HCT116 cells, & WCEs were tested in precipitation assays. Note that the mutant pVHL-W88S almost completely lost the ability to bind to HIF-1 alpha & HIF-2 alpha, whereas pVHL-Y112H retains this ability almost completely. However, both pVHL-W88S & pVHL-Y112H show significantly reduced binding to FASN & CAD, as indicated by small & large asterisks, respectively. Arrows indicate HIF-1 alpha & HIF-2 alpha bands. Long arrows & arrowheads indicate unmodified & modified forms of FASN, respectively. Protein samples prepared from hypoxia (1% oxygen)-exposed cells were added as positive control in left-end extra lanes. (C) The same samples as used in B were analyzed by silver staining. The E3 ligase components appear as major bands as indicated. (D) Subcellular localization of stably expressed pVHL mutants in 786-O. pVHL-Y112H & pVHL-W88S showed preferential nuclear localization, whereas pVHL-WT & pVHL-H115Q which both strongly bind to FASN & CAD, showed cytoplasmic localization. Cells showing clear nuclear (C N) localization in three microscopic viewing areas were counted as shown in the lower panel. The total counted cell numbers ranged from 35 to 64. The experiments were repeated more than twice, except for the cell localization count, & the results were reproduced. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28775317), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-479_rabbit-polyclonal-hif-1-alpha-antibody-310202415541984.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] -" data-caption="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] - Hypoxia signaling & cytotoxicity to CdCl2. (a) Wild-type (WT) cells were left untreated (Ctrl) or exposed to 150 μM CoCl2 (Co2+), 1, 5 or 10 μM CdCl2 (Cd2+) or 150 μM CoCl2 & 10 μM CdCl2 for 24 hours. HIF1 alpha −/− cell extract treated with 150 μM CoCl2 was used as a negative control. Nuclear protein was extracted & separated by SDS-PAGE, transferred to nitrocellulose membrane & probed with a HIF1 alpha (upper panel) or beta-actin (lower panel) specific antibody. The bands observed in the cadmium only WT cells are nonspecific as they are also observed in the HIF1 alpha −/− cells. (b) BNip3 mRNA expression levels were analyzed by qRT-PCR in wild type (WT, white bars) & HIF1 alpha −/− cells (black bars). Cells were left untreated (0), or exposed to 5 μM CdCl2 (Cd2+) or 150 μM CoCl2 (Co2+) for 24 hours. Each value was normalized to the control level in the corresponding cell line. *P < 0.05 compared to the corresponding controls, n = 4. (c) BNIP3 protein levels were determined in wild type & HIF1 alpha −/− cells after treatment with 150 μM CoCl2 (Co2+) or 5 μM CdCl2 (Cd2+) for 24 hours using a BNIP3 specific antibody & beta-actin was used as a loading control (lower Panel). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/21811500), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-479_rabbit-polyclonal-hif-1-alpha-antibody-310202415532086.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody - BSA Free [NB100-479] -" data-caption="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody - BSA Free [NB100-479] - FIH exits the nucleus via a Leptomycin B-sensitive exportin1-dependent pathway. (A) Immunofluorescence staining of FIH (green) & HIF1 alpha (red) in MCF7 cells after the indicated hypoxia (0.5% O2) & re-oxygenation treatments. TO-PRO-3 (blue) was used to stain nuclei. (B) Total cell lysates from U2OS cells were immunoprecipitated with an anti-exportin1 antibody or control IgG. FIH, exportin1 & beta-tubulin levels are indicated. (C) Immunofluorescence staining of FIH (green) in FIH-null mouse embryonic fibroblasts (MEFs) transfected with HA-FIH 1–349 or HA-FIH deltaNES followed by normoxia, hypoxia (1% O2, 3 h) or 3 h of hypoxia followed by re-oxygenation for 1 h. TO-PRO-3 (blue) was used to stain nuclei. Arrows indicate nuclear localization of signal. (D) Total cell lysates from U2OS cells transfected with control vector, HA-FIH 1–349 or HA-FIH deltaNES were immunoprecipitated with an anti-exportin 1 antibody. HA-FIH, exportin1 & beta-tubulin levels are indicated. FL, full length; IgGL, IgG light chain. Scale bars: 20 µm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30333145), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Immunocytochemistry/ Immunofluorescence" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-479_rabbit-polyclonal-hif-1-alpha-antibody-31020241554549.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] -" data-caption="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] - Phagocyte infiltration contributes to the respiratory burst & consequent EPO signalling activation during inflammation resolution.ZymA (i.p., 1 mg per mouse) was applied to induce peritonitis in male WT mice. (a–c) Mice were treated with either 0.5 mg per mice i.p. of anti-Ly6G antibody or control (isotype control rat IgG2a) 1.5 day before zymA injection, & exudate leucocytes, peritoneum & peritoneal fluid were collected for analysis of ROS (a, n=3), hypoxia, HIF-1 alpha (b, n=3) & EPO (c, n=3) at 6 24 h. (d–f) Mice were treated with either anti-Ly6G antibody (0.5 mg per mice) plus clodronate liposomes (0.2 ml per 10 g) or control (isotype antibody+empty liposome) before zymA injection, & exudate leucocytes, peritoneum & peritoneal fluid were collected for analysis of ROS (d, n=3), hypoxia, HIF-1 alpha (e, n=3) & EPO (f, n=3) at 24 h. Representative data from two independent experiments are shown. For flow cytometry data, black numbers refer to the percentage of positive cells & red numbers refer to the mean fluorescent intensity. Error bars represent the s.e.m. *P" data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-479_rabbit-polyclonal-hif-1-alpha-antibody-310202415532065.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Chromatin Immunoprecipitation: HIF-1 alpha Antibody - BSA Free [NB100-479] -" data-caption="Chromatin Immunoprecipitation: HIF-1 alpha Antibody - BSA Free [NB100-479] - Hypoxia reduces inflammatory signaling pathways & NLRP3 expression & induces autophagy in IECs. a HT-29 cells were subjected to normoxia & hypoxia at the indicated times in the absence or presence of 10 µg/ml LPS. Autophagy was measured by variations in the ratio of LC3-II/LC3-I & the total amount of LC3 (LC3-I plus LC3-II) relative to beta-actin. Results are representative of two independent experiments. b, c & d HT-29 cells were subjected to normoxia & hypoxia for the indicated periods in the absence or presence of 10 µg/ml LPS, followed by transcript analysis. Statistical analysis was performed using one-way ANOVA followed by Tukey’s post-test. Results represent mean + s.e.m. of two independent experiments done in triplicate, *P " data-badges="" data-application="Chromatin Immunoprecipitation" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-479_rabbit-polyclonal-hif-1-alpha-antibody-310202415533816.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] -" data-caption="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] - Effect of Hif1a deletion upon growth & invasion. (A) Wild-type (WT) mammary tumor epithelial cells (MTECs) grown to 80% confluence subjected to hypoxic culture for the indicated times up to 24 hours, or cells continued to be cultured under normoxic conditions such that the time t = 0 sample harvested on the same day as the time t = 24 hours hypoxic condition sample. High-salt enriched whole-cell extracts resolved on 3% to 8% Tris-acetate gels & blotted onto polyvinylidene fluoride membrane, which divided horizontally at approximately 60 kDa. The top half of the blot used to detect HIF-1 alpha, & the lower portion used to detect lamin (loading control) to avoid the need to strip & reprobe the blot. (B) Growth curve of WT & knockout (KO) MTECs cultured at normoxia (Nor) or hypoxia (Hyp) in growth medium supplemented w/ 5% fetal bovine serum (FBS) + epidermal growth factor (EGF) (left) or w/ 2% FBS (right). For cells grown in 5% FBS + EGF, a representative graph is shown in which the mean ± SEM of cell number per time point of quadruplicate wells per genotype/oxygen tension is plotted per time point. For cells grown in 2% FBS, the grand mean ± SEM of cell number is presented, which calculated as an average of the mean cell number observed per replicates per time point as observed in three replicate experiments. All data analyzed by two-way analysis of variance (ANOVA, *P < 0.05). (C) The mean fold change (FC) in invasion normalized to invasion index observed for WT cells cultured at normoxia (FC = 1.0). Data represent the mean FC in invasion observed in three independent experiments. All columns compared using one-way ANOVA w/ a Bonferroni posttest. *P < 0.05. Image collected & cropped by CiteAb from the following publication (http://breast-cancer-research.biomedcentral.com/articles/10.1186/bcr3087), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-479_rabbit-polyclonal-hif-1-alpha-antibody-310202415541997.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] -" data-caption="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] - Involvement of HIF-1 alpha in alveolar epithelial cell phenotypic changes induced by hypoxia. Primary rat AECs were cultured in normoxia (Nx) (21% O2) or hypoxia (Hx) (1.5% O2) during 6 days in presence or absence of 10 µM YC-1. (A) Immunostaining of ZO-1 (magenta) & TTF1 (cyan) were performed. n = 4 experiments were performed. Isolated primary rat AECs were cultured in normoxia (Nx) (21% O2) or hypoxia (Hx) (1.5% O2) during 6 h in the presence or absence of 100 mM 4-phenylbutyrate (4-PBA) or pre-treated or not with 1 µM BAPTA-AM 90 min before exposition to hypoxia. A representative picture of at least n = 4 independent experiments for each condition has been presented & scale bar represents 50 µm. (B) Western blot of HIF-1 alpha protein levels was performed. Representative blot of n = 5 experiments is shown. Expression levels of HIF-1 alpha were quantified & reported to beta-actin expression for each condition. Primary rat AECs were transfected with plasmid coding for luciferase reporter activity of hypoxia responsive element (HRE: i.e., HIF-luc), & cultured as described. (C) Luciferase activity corresponding to the transcriptional capacity of HIF was reported (n = 4 experiments). Raw data were submitted a Kruskal-Wallis test. * & ** indicate a significant difference as compared with normoxic value with p < 0.05 & p < 0.01 respectively. # & ## indicate a significant difference as compared with value in untreated hypoxic cells with p < 0.05 & p < 0.01, respectively. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30875855), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-479_rabbit-polyclonal-hif-1-alpha-antibody-31020241554511.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] -" data-caption="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] - Pharmacological inhibition of ABCC3 reduces cell proliferation through STAT3 & HIF1 alpha dysregulation & induction of apoptosis. a Representative Western blot images show the effects of pharmacological inhibition of ABCC3 with MCI-715 on the expression of pSTAT3 Y705 & HIF1 alpha in three PDAC cell lines (AsPC1, HPAFII, CFPAC-1). Cells were treated with MCI-715 at the concentration of 10 μM & collected after 24 h (CFPAC-1) or 48 h (AsPC1, HPAFII). The quantitative analysis of n = 3 separate experiments is presented in Additional file 1: Figure S2; b The effects of the treatment of AsPC1, HPAFII & CFPAC-1 cell lines with 10 μM MCI-715 on the Caspase 3/7 activity (72 h post treatment) measured with Caspase 3/7 fluorigenic probe; c Representative Western blotting images & quantitative analysis of cleaved caspase 3 expression following treatment of indicated PDAC cell lines with 10 μM MCI-715. All results are presented as mean ± SEM of 3 independent experiments. The quantitative analysis was performed with the use of ImageJ & Image Lab software, unpaired Student’s t-test was performed for statistical analysis, *p " data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-479_rabbit-polyclonal-hif-1-alpha-antibody-310202415525223.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] -" data-caption="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] - Normoxic pretreatment sensitizes glioma cells to radiation after rapid acute hypoxic (RAH) exposure.(A) The rapid acute hypoxia (RAH) protocol is shown depicting the timing & severity of hypoxic exposure. Cells either remain in a continuous hypoxic environment (–) or are transiently (25 min) exposed to normoxia 25 min prior to radiation (+). Continuously normoxic cells (NOx) were irradiated as a positive control. (B) The results of anchorage-independent colony forming assays are shown for U87, U87-luc, GL261 glioma cells & 0308 GSCs after 5 Gy radiation exposure under varying oxygen conditions. To allow for ease of comparisons among cell types, raw values are expressed as a percentage of the corresponding cell type’s negative (non-irradiated) control & the means & SEMs are plotted. Each result represents at least three independent samples, plated in triplicate. Holm-Sidak comparisons for multiple groups were used for statistical comparisons of raw values (**p" data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-479_rabbit-polyclonal-hif-1-alpha-antibody-310202415535197.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] -" data-caption="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] - Multiple distinct mechanisms for downregulation of AKT activity in TSC1/2-deficient SCs. A-F: Western blots of sciatic nerve lysates from control & TSC2-SCKO/TSC1-SCKO mice at age P28 (A-E) or P14 (F) (3 mice per group), probed with the indicated antibodies. G: Immunofluorescence of longitudinal frozen sciatic nerve sections from control & TSC2-SCKO mice (age P28) showing greatly increased immunoreactivity of PTEN in the mutant (cytoplasmic signals surrounding DAPI+ nuclei). Scale bars: 50 µm H: Schematic summarizing distinct mechanisms underlying the downregulation of AKT activity in SCs from TSC1/2-SCKO nerves. Components highlighted in red are upregulated while blue highlighting depicts downregulation in mutant nerves. Note downregulation of insulin receptor substrate 1 (IRS-1) by constitutively active S6 kinases downstream of mTORC1. This leads to attenuated activation of PI3K/AKT. Lack of mTORC2 stimulation occurs through abolished TSC1/2 which results in reduced AKT phosphorylation in position Ser473 as well as through feedback inhibition by S6 kinases. Lastly, hyperactive mTORC1 promotes Hif1 alpha transcription factor expression which results in increased PTEN transcription, & thus attenuated PI3K/AKT signaling. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29497474), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-479_rabbit-polyclonal-hif-1-alpha-antibody-310202415525279.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] -" data-caption="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] - DLK1 cleavage is dependent on HIFs & ADAM 17 activity.a, b Representative images & densitometric analysis of western blots showing HIF-1 alpha, HIF-2 alpha, & DLK1 expression & cleavage in U3082MG cells after siRNA targeting of HIF1A & HIF2A in hypoxia. c, d Representative images & densitometric analysis of western blots showing the effects of ADAM inhibition by pre-treatment with 20 µM TAPI-2 on DLK1 cleavage in U3082MG cells grown at 21% or 1% O2 for 48 h. SDHA was used as loading control. e, f Representative images & densitometric analysis of western blots showing the effects of ADAM17 inhibition by pre-treatment with 0.5 μM TMI-1 on DLK1 cleavage in U3082MG cells grown at 21% or 1% O2 for 48 h. SDHA was used as loading control. Statistical analysis: b has 3 independent experiments while d & f have four independent experiments, all data are expressed as mean ± SEM. Statistical significance was determined by one-way ANOVA, followed by Bonferroni post hoc test. In the whole figure significance is represented as *p " data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-479_rabbit-polyclonal-hif-1-alpha-antibody-310202415535159.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] -" data-caption="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] - Hypoxia downregulates MHC class I expression via HIF transcription factors.(A-C): siRNA mediated knockdown of HIF-1 alpha reversed hypoxic downregulation of MHC class I expression as compared with the scrambled, non-targeting (NT) siRNA control. MCA205 tumor cells were reverse transfected with scrambled siRNA (NT; red histogram) or with HIF-1 alpha specific siRNA (blue histogram) & cultured as 3D spheroids under 1% (A) or 21% (B) oxygen for 48h. Levels of MHC class I surface expression was determined using flow cytometry. Efficacy of gene knockdown was assessed using Western blot (C). beta-Actin was used as the loading control. Representative data of 3 independent experiments shown. (D-F): Flow cytometry assessment of surface expression of HLA-ABC on paired isogenic renal cell carcinoma cell lines RCC4 (D), UMRC2 (E) & CAKI2 (F). Each pair had the parental cell line that lacked endogenous wild-type VHL (VHL null, transfected with empty vector) & one with vector stably expressing functional VHL (VHL restored). Restoring VHL function & thereby reducing HIF expression, significantly increased HLA-ABC expression on the cells. Representative histograms of 4 independent experiments are shown. Grey filled: unstained control; red: VHL null genotype; blue: VHL restored genotype. (D1-F1): Inactivation of HIF-1 alpha by restoring VHL expression was verified by Western blotting for RCC4 (D1), UMRC2 (E1) & CAKI2 (F1) cells. beta-Actin was used as the loading control. Image collected & cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0187314), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-479_rabbit-polyclonal-hif-1-alpha-antibody-310202415535179.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] -" data-caption="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] - Effect of ET-1 & Cx43 on HIF-1 alpha expression & glucose uptake when c-Src is inhibited.Astrocytes were preincubated with 100 ng/µL PP2 (c-Src inhibitor) or 100 ng/µL PP3 (inactive analogue) for 1 h. Then, cells were incubated in the absence (control) or presence of 0.1 µM ET-1 for 24 h. A) HIF-1 alpha Western blot & quantification. The results are expressed as percentages of the level found in the controls treated with PP3 & they show that the inhibitor of c-Src PP2 prevented the up-regulation of HIF-1 alpha promoted by ET-1. ***p" data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-479_rabbit-polyclonal-hif-1-alpha-antibody-310202415533821.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] -" data-caption="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] - HIF-1 alpha protein increases in HCT116 cells exposed to rapid intermittent hypoxia. Increased HIF-1 alpha increases HIF-1 transactivation, leading to increased expression of HIF-1 target genes at both the mRNA & protein level. (A) Rapid intermittent hypoxia increases HIF-1 alpha protein after 6 h of exposure. (B) Rapid intermittent hypoxia increases the mRNA expression of genes involved in glycolysis, extracellular matrix remodeling & the HIF pathway after 18 h of exposure. (C) Additional genes with known hypoxia response elements (HRE) binding sites for HIF-1 were measured & all tested genes showed an increase in both chronic hypoxia & intermittent hypoxia after 18 h. All values were normalized to normoxic expression levels, which is equivalent to 0 on the Log2 scale. Results are the mean ± SEM of independent experiments run in duplicate (n ≥ 3). (D) The increase in mRNAs is also reflected in an increase in the protein levels of Glut1, HK2, LDHA & PHD2. * p < 0.05, ** p < 0.01, *** p < 0.001 & **** p < 0.0001. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30669593), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-479_rabbit-polyclonal-hif-1-alpha-antibody-31020241555237.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] -" data-caption="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] - Hif1 alpha expression in neonatal & adult testis.(A) Section of testis from 5-day old (P5) male new born pups showing Hif1 alpha expression in MVH+ gonocytes within the seminiferous tubules (top) & negative control images without primary antibodies (bottom). Scale bar: 50μm. (B) Western blot analysis of Hif1 alpha expression in P5 testes (left) compared to extract of the adult brain sub-ventricular zone (SVZ) (right). Loading control ( beta-Actin) is shown below. (C) Section of adult (3 month old) testis showing Hif1 alpha expression in spermatogonia. Scale bar: 30μm. (D) Western blot analysis of whole adult testis. HEK293 cells treated with DFX were used as a positive control & intestinal tissue was used as a negative control. Loading control ( beta-Actin) is shown below. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27148974), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-479_rabbit-polyclonal-hif-1-alpha-antibody-310202415535183.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] -" data-caption="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] - Roles of P38 mitogen-activated protein kinase (MAPK) in the DFO-mediated up-regulate of HIF-1 alpha protein in APPsw cells. (A) APPsw were exposed to 100 μM Fe, 100 μM DFO, or both combined for 24 h. Whole cell lysates were prepared & subjected to analysis for P38 or GAPDH protein. (B) Western blot analysis revealed that strong phosphorylation of P38 MAPK was detected in the DFO-treated cells, whereas no significant changes were observed in cells treated Fe alone compared with control. There were not statistical changes in the levels of P38 MAPK among the groups. The data represent the mean ± S.E. of three independent experiments. *p < 0.05, **p < 0.01 compared with the control; #p < 0.05, ##p < 0.01 with respect to the Fe treatment group. (C–E) APPsw cells were pretreated without or with SB203580 (10 μM) for 2 h & then treated without or with DFO for additional 22 h in the absence or presence of the inhibitor. Immunoblotting showed that DFO treatment significantly increased the levels of p-P38 MAPK compared with the control group, whereas the increase was significantly ameliorated by SB203580. Furthermore, the DFO-mediated up-regulation of HIF-1 alpha protein was greatly inhibited by SB203580. The data represent the mean ± S.E. (n = 3). **p < 0.01 compared with the control; ##p < 0.01 compared to the values of DFO treatment group (two-way ANOVA with Post hoc Fisher’s PLSD). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26082716), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-479_rabbit-polyclonal-hif-1-alpha-antibody-310202415533826.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] -" data-caption="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] - Expression of hypoxia-inducible factor (HIF) proteins after bilateral renal ischemia or sham ischemia. Immunoblots for HIF-1 alpha (A–C) & HIF-2 alpha (D–F) of tissue extracts from the cortex & outer & inner medulla of the left kidneys of rats 24 h & 5 days following recovery from either sham ischemia (○) or bilateral renal ischemia (●); n = 6 per group. G: typical image of the gel following electrophoresis. H: typical image of the nitrocellulose membrane following transfer. Values are expressed as medians (25th percentile, 75th percentile). Paired comparisons were performed using the Mann-Whitney U-test. Because paired comparisons were made at two time points, P values were conservatively adjusted using the Dunn-Sidak method with k = 2. PTr, PT, & PTr*T are the outcomes of two-way analysis of variance on ranking with the factors treatment (Tr) & time (T). AU, arbitrary unit; I1, 24 h after ischemia; I5, 5 days after ischemia; S1, 24 h after sham ischemia; S5, 5 days after sham ischemia. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30110566), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-479_rabbit-polyclonal-hif-1-alpha-antibody-310202415533840.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] -" data-caption="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] - DFO regulates the expression of HIF-1 alpha & HIF-1-related protein in the brains of APP/PS1 mice. (A) Western blots showing the expression levels of HIF-1 alpha protein in control & DFO-treated transgenic mice brains. (B) Immunoblot showed that DFO treatment significantly increased HIF-1 alpha protein levels, compared with controls. (C) The expression levels of HIF-1 alpha mRNA were detected by RT-PCR in the brains of APP/PS1 transgenic mice treated with DFO. (D) RT-PCR analysis showed that DFO treatment significantly increased the HIF-1 alpha mRNA levels in the transgenic mouse brain. GAPDH served as the internal control. (E) Immunohistochemically stains showed the distribution of HIF-1 alpha in the cortical & hippocampus sections of APP/PS1 mouse brain (Scale bar = 100 μm). (F) Western blots showing the expression levels of brain-derived neurotrophic factor (BDNF), DMT1, & TFR proteins in APP/PS1 transgenic mice brains 3 months after DFO administration. GAPDH was used as a loading control. (G–J) DFO treatment led to a marked increase in the BDNF, DMT1 + IRE, & TFR protein levels in the brains of transgenic mice compared with the control. There was no significant change in the expression levels of DMT1-IRE protein between the groups, compared with the control. Data represent the mean ± S.E. of 6 independent experiments. **P < 0.01 vs. control group. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26082716), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-479_rabbit-polyclonal-hif-1-alpha-antibody-310202415525237.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] -" data-caption="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] - Phagocyte infiltration contributes to the respiratory burst & consequent EPO signalling activation during inflammation resolution.ZymA (i.p., 1 mg per mouse) was applied to induce peritonitis in male WT mice. (a–c) Mice were treated with either 0.5 mg per mice i.p. of anti-Ly6G antibody or control (isotype control rat IgG2a) 1.5 day before zymA injection, & exudate leucocytes, peritoneum & peritoneal fluid were collected for analysis of ROS (a, n=3), hypoxia, HIF-1 alpha (b, n=3) & EPO (c, n=3) at 6 24 h. (d–f) Mice were treated with either anti-Ly6G antibody (0.5 mg per mice) plus clodronate liposomes (0.2 ml per 10 g) or control (isotype antibody+empty liposome) before zymA injection, & exudate leucocytes, peritoneum & peritoneal fluid were collected for analysis of ROS (d, n=3), hypoxia, HIF-1 alpha (e, n=3) & EPO (f, n=3) at 24 h. Representative data from two independent experiments are shown. For flow cytometry data, black numbers refer to the percentage of positive cells & red numbers refer to the mean fluorescent intensity. Error bars represent the s.e.m. *P" data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-479_rabbit-polyclonal-hif-1-alpha-antibody-31020241554533.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Immunohistochemistry: HIF-1 alpha Antibody - BSA Free [NB100-479] -" data-caption="Immunohistochemistry: HIF-1 alpha Antibody - BSA Free [NB100-479] - A “white-jade” muscle phenotype in EV-A71-infected mouse strain 129. aLeft panel: We coined a “white-jade” phenotype to describe the pathological changes in the color of the hindlimb muscle in EV-A71-infected mice (indicated by arrow). This phenotype showed characteristic muscle with locally whitened color in appearance & hardened tissue mass. In the saline control, tendons (not muscle) can be seen in white color. Right panel: An anatomical sketch of the hindlimb muscle. GM: gluteal muscle, QF: quadratus femoris muscle, BF: Biceps femoris muscle. b Both VP1 protein (upper) & myogenin protein (lower) were detected by IHC staining in the muscles, indicating EV-A71 infection, muscle injury & regeneration. The magnification is 200X. c Striking expression of HIF1A (hypoxia inducible factor 1-alpha) protein (brown color) was detected by IHC staining in the white-jaded tissue, but not in the non-white-jaded tissue from the same mouse. d Specific association between HIF1A expression & white-jaded muscle Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31711481), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Immunohistochemistry" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-479_rabbit-polyclonal-hif-1-alpha-antibody-31020241554523.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] -" data-caption="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] - Kidney-derived Sca-1+ cells produce Epo. (A) Epo protein levels in supernatants obtained from Sca-1+ cells cultured in normoxia or hypoxia (n = 3 biological replicates). (B) HIF-1 alpha & HIF-2 alpha protein levels in Sca-1+ kidney-derived cells after exposure to normoxia (20% O2) or hypoxia (1% O2). Numbers below the blots indicate the fold change of the ratio of HIF-1 alpha/Tubulin & HIF-2 alpha/Tubulin in 1% O2 to the respective 20% O2 control. (C) Epo RNA levels in Sca-1+ cells that were incubated either at 20% O2 of 1% O2 as indicated (n = 4 biological replicates). (D) Mesenchymal stem cell markers in renal Sca-1+ cells on day 21 of culture; reads per kilobase million (RPKM). (E) Volcano plot of 4592 significantly up- & 5376 significantly downregulated mRNAs in kidney-derived Sca-1+ cells on day 21 in culture compared to day 0. Mean values ± SEM are shown. * p < 0.05. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/35203399), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-479_rabbit-polyclonal-hif-1-alpha-antibody-310202415532023.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] -" data-caption="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] - Hif1 alpha expression in neonatal & adult testis.(A) Section of testis from 5-day old (P5) male new born pups showing Hif1 alpha expression in MVH+ gonocytes within the seminiferous tubules (top) & negative control images without primary antibodies (bottom). Scale bar: 50μm. (B) Western blot analysis of Hif1 alpha expression in P5 testes (left) compared to extract of the adult brain sub-ventricular zone (SVZ) (right). Loading control ( beta-Actin) is shown below. (C) Section of adult (3 month old) testis showing Hif1 alpha expression in spermatogonia. Scale bar: 30μm. (D) Western blot analysis of whole adult testis. HEK293 cells treated with DFX were used as a positive control & intestinal tissue was used as a negative control. Loading control ( beta-Actin) is shown below. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27148974), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-479_rabbit-polyclonal-hif-1-alpha-antibody-310202415523915.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] -" data-caption="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] - Evidence that hypoxia induces nuclear entry of FIH. (A) Protein levels of HIF1 alpha, HIF2 alpha, FIH & PHD2 in U2OS cells during hypoxia (1% O2) treatment at the indicated time points. GAPDH was used as a loading control. (B) Immunofluorescence staining of FIH (green) in U2OS cells under hypoxic conditions (1% O2) at the indicated time points. TO-PRO-3 (blue) was used to stain nuclei. (C) Protein levels of FIH & HIF1 alpha from cytoplasmic or nuclear fractions in U2OS cells in normoxia or hypoxia (1% O2, 3 h). beta-tubulin & PARP were used as loading controls for the cytoplasmic & nuclear fractions, respectively. Figures beneath lanes 2 & 4 indicate relative intensities of nuclear FIH in normoxia & hypoxia. Note that different quantities of cytoplasmic & nuclear extracts were loaded. Scale bars: 20 µm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30333145), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-479_rabbit-polyclonal-hif-1-alpha-antibody-31020241612619.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] -" data-caption="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] - Respiratory burst contributes to the EPO signalling activation during inflammation resolution.ZymA (i.p., 1 mg per mouse) was applied to induce peritonitis in male WT mice or CGD mice. (a) Exudate leucocytes & peritoneum were collected at indicated intervals, & protein levels of HIF-1 alpha & HIF-2 alpha were measured by WB (n=3). (b) YC-1, 400083 or 400087 were intraperitoneally given to WT mice 3 h before zymA injection & the protein levels of related molecules were analysed by WB (n=2). (c) Mice were killed 1 h after injection of pimonidazole at indicated intervals & the protein adducts of reductively-activated pimonidazole were detected by WB with Hypoxiprobe-1-Mab-1 (HPI) (n=2). (d) Flow cytometry for ROS at indicated intervals (n=3). (e) Flow cytometry for cellular sources of ROS at indicated intervals (n=3). Representative data from at least two independent experiments are shown. For flow cytometry data from d, black numbers refer to the percentage of positive cells & red numbers refer to the mean fluorescent intensity. Error bars represent the s.e.m. *P" data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-479_rabbit-polyclonal-hif-1-alpha-antibody-31020241555246.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] -" data-caption="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] - Effect of IKK2 inhibition on HUVEC, microvessel outgrowth from aortic rings, & VEGF-A expression. a Dose-dependent (10, 5 & 2.5 ng/ml) inhibitory effect of IMD0354 on HUVEC migration relative to control (DMSO 1 µl/ml). Living HUVEC were visualized with Calcein-AM (green). b Quantification of HUVEC migration distance (n = 8). One-way ANOVA test with Tukey multiple comparison was used to determine statistical significance. c HUVEC has grown on Geltrex to evaluate tube formation in the presence of IMD0354 compared to drug-free vehicle (DMSO). Vital HUVEC are stained with calcein-AM (green), & dead HUVEC are displayed in red (propidium iodide). Quantitative analysis of a number of junctions (d), & tubules (e) formed by HUVEC, & total tubule length (f) (n = 8). g Quantitative analysis of cell death induced by IMD0354 treatment (n = 8). h Effect of IMD0354 on cell proliferation in the aortic ring assay. Student t test was used to determine statistical significance. i Western blot analysis of VEGF-A expression in HUVEC treated with IMD0354 (10, 5, 2.5 ng/ml), with beta-actin as a loading control. j Western blot analysis of HIF-1 alpha expression in HUVEC treated with IMD0354 (10, 5, 2.5 ng/ml), with beta-actin as a loading control. k Immunofluorescent detection of VEGF-A (green) in HUVEC treated with IMD0354 (10, 5, 2.5 ng/ml). Cell nuclei visualized with DAPI staining (blue). n.s. p > 0.05; *p " data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-479_rabbit-polyclonal-hif-1-alpha-antibody-310202415535114.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] -" data-caption="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] - The duration of enhanced radiosensitivity after normoxic pretreatment of cells undergoing Rapid Acute Hypoxia.(A) The RAH protocol is shown with variable delays to radiation after normoxic pretreatment. In this protocol all cells are transiently (25 min) exposed to normoxia for 25 min (+) & then returned to severe hypoxia (1% O2) for 1, 3, or 6 hours prior to radiation. Continuously normoxic cells (NOx) were irradiated as a positive control. (B) Results from anchorage-independent colony forming assays indicate that the decay of enhanced radiosensitivity for cells in the RAH protocol is generally more rapid than that observed for cells in the GCH protocol. To allow for ease of comparisons among cell types, raw values are expressed as a percentage of the corresponding cell type’s negative (non-irradiated) control & the means & SEMs are plotted. Each result represents at least three independent samples, plated in triplicate. Holm-Sidak comparisons for multiple groups were used for statistical comparisons of raw values (*p" data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-479_rabbit-polyclonal-hif-1-alpha-antibody-31020241682112.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] -" data-caption="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] - HIF-1 alpha overexpression rescues oxygen-induced radioresistance in RAH-treated cells, but not GCH-treated cells.Results are shown for the anchorage-independent colony forming assays for U87 cells transfected with either an empty vector or HIF-1 alpha expression vector & then exposed to GCH or RAH protocols without (–) or with (+) reoxygenation. Continuously normoxic cells (NOx) were irradiated as a positive control. To allow for ease of comparisons among conditions, raw values are presented as a percentage of that cell type’s negative (non-irradiated) control & the means & SEMs are plotted. Each result represents at least three independent samples, plated in triplicate. Holm-Sidak comparisons for multiple groups were used for statistical comparisons of raw values (*p" data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-479_rabbit-polyclonal-hif-1-alpha-antibody-31020241612637.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] -" data-caption="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] - ONECUT2 modulates HIF1 alpha binding to chromatin in NE-like PC3 cells. a Western blot of HIF1 alpha & ONECUT2 in PC3 cells under normoxic & hypoxic conditions. Two different siRNAs targeting ONECUT2 were mixed together for knockdown experiments. b Left panel: heatmaps show HIF1 alpha ChIP-Seq signal in PC3 cells under hypoxic conditions with & without knockdown of ONECUT2; right panel: pileup of HIF1 alpha ChIP-Seq signals centered at HIF1 alpha ChIP-Seq peaks center. c Expression of ANGPTL4 & ADM, two hypoxia-regulated genes, with & without knockdown of ONECUT2 in PC3 cells. d Schematic illustration of the analysis identifying SMAD3 as an ONECUT2 regulated HIF1 alpha co-factor. Genes identified in motifs enriched in HIF1 alpha binding sites & ONECUT2 target genes were further filtered by HIF1 alpha interacting protein list from BioGRID. ONECUT2 target genes were defined as differentially expressed in ONECUT2 knockdown & control samples & with ONECUT2 binding sites nearby in PC3 cells under hypoxic conditions. e SMAD3 expression in response to ONECUT2 silencing in PC3 cells. f SMAD3 & HIF1 alpha binding sites with & without silencing of SMAD3. g The overlap of SMAD3 ChIP-Seq, HIF1 alpha ChIP-Seq & SMAD3-HIF1 alpha ChIP-re-ChIP-Seq peaks. h SMAD3 ChIP-Seq, HIF1 alpha ChIP-Seq & SMAD3-HIF1 alpha ChIP-re-ChIP-Seq signal near the promoter regions of hypoxia-induced genes ANGPTL4 & ADM. Error bars indicate s.d. from at least two technical replicates. P-value is calculated by one-way ANOVA. **: P " data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-479_rabbit-polyclonal-hif-1-alpha-antibody-310202415525245.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] -" data-caption="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] - The duration of enhanced radiosensitivity after normoxic pretreatment differs among cell lines undergoing Graded Chronic Hypoxia.(A) The GCH protocol is shown with variable delays to radiation after normoxic pretreatment. After GCH, all cells are transiently (25 min) exposed to normoxia for 25 min (+) & are then returned to severe hypoxia (1% O2) for 1, 3, or 6 hours prior to radiation. Continuously normoxic cells (NOx) were irradiated as a positive control. (B) Results from anchorage-independent colony forming assays indicate that the decay of enhanced radiosensitivity differs among cell lines. To allow for ease of comparisons among cell types, raw values are expressed as a percentage of the corresponding cell type’s negative (non-irradiated) control & the means & SEMs are plotted. Each result represents at least three independent samples, plated in triplicate. Holm-Sidak comparisons for multiple groups were used for statistical comparisons of raw values (*p" data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-479_rabbit-polyclonal-hif-1-alpha-antibody-31020241553718.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] -" data-caption="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] - Expression of hypoxia-inducible factor (HIF) proteins after bilateral renal ischemia or sham ischemia. Immunoblots for HIF-1 alpha (A–C) & HIF-2 alpha (D–F) of tissue extracts from the cortex & outer & inner medulla of the left kidneys of rats 24 h & 5 days following recovery from either sham ischemia (○) or bilateral renal ischemia (●); n = 6 per group. G: typical image of the gel following electrophoresis. H: typical image of the nitrocellulose membrane following transfer. Values are expressed as medians (25th percentile, 75th percentile). Paired comparisons were performed using the Mann-Whitney U-test. Because paired comparisons were made at two time points, P values were conservatively adjusted using the Dunn-Sidak method with k = 2. PTr, PT, & PTr*T are the outcomes of two-way analysis of variance on ranking with the factors treatment (Tr) & time (T). AU, arbitrary unit; I1, 24 h after ischemia; I5, 5 days after ischemia; S1, 24 h after sham ischemia; S5, 5 days after sham ischemia. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30110566), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-479_rabbit-polyclonal-hif-1-alpha-antibody-310202415535138.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] -" data-caption="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] - Respiratory burst contributes to the EPO signalling activation during inflammation resolution.ZymA (i.p., 1 mg per mouse) was applied to induce peritonitis in male WT mice or CGD mice. (a) Exudate leucocytes & peritoneum were collected at indicated intervals, & protein levels of HIF-1 alpha & HIF-2 alpha were measured by WB (n=3). (b) YC-1, 400083 or 400087 were intraperitoneally given to WT mice 3 h before zymA injection & the protein levels of related molecules were analysed by WB (n=2). (c) Mice were killed 1 h after injection of pimonidazole at indicated intervals & the protein adducts of reductively-activated pimonidazole were detected by WB with Hypoxiprobe-1-Mab-1 (HPI) (n=2). (d) Flow cytometry for ROS at indicated intervals (n=3). (e) Flow cytometry for cellular sources of ROS at indicated intervals (n=3). Representative data from at least two independent experiments are shown. For flow cytometry data from d, black numbers refer to the percentage of positive cells & red numbers refer to the mean fluorescent intensity. Error bars represent the s.e.m. *P" data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-479_rabbit-polyclonal-hif-1-alpha-antibody-310202415541939.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] -" data-caption="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] - G9a inhibition reduces cell motility in breast cancer cell lines. G9a inhibition represses breast cancer cell motility in vitro. (A) Microarray analysis of differentially expressed genes comparing hypoxia-responsive genes from MCF7 cells expressing shNS & shG9a identifies a subset of 212 genes downregulated in hypoxia in a G9a-dependent manner. (B) Ingenuity Pathway Analysis results of the 212 G9a-dependent genes. (C) Evaluation of the migratory distance covered by MDA-MB-231 treated with UNC0642 (5 μM) or (D) transfected with either vector or shG9a. Results were evaluated through real-time imaging using the HoloMonitor M4, taking pictures every 10 minutes for 48 hours, in three independent experiments. An average of 20 cells per condition is shown for the representative circular displacement images. (E) Western blot analysis of G9a, H3K9me1 & H3K9me2 in MDA‑MB-231 transfected with vector or shG9a & incubated in hypoxia for 24 hours. HIF1 alpha was included as positive control for hypoxic conditions. H3 & Lamin A/C were used as loading control (F) Scratch wound assay for MDA-MB-231 breast cancer cells treated with 5 μM UNC0642, under both normoxic (21% O2) & hypoxic (1% O2) conditions. Results were evaluated by real-time imaging performed by the IncuCyte Zoom every 24 hours & wound closure was quantified using ImageJ. Scale bar represents 500 μm. (G) Scratch wound assay of MCF7 breast cancer cells following G9a KD & G9a reconstitution. Scale bar represents 500 μm. (H) Western blot analysis of MCF7 cells transfected with shG9a & reconstituted with WT G9a. Data are represented as mean ± SEM of three independent experiments (unpaired, non-parametric Student's t-test, **p" data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-479_rabbit-polyclonal-hif-1-alpha-antibody-31020241554513.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Chromatin Immunoprecipitation: HIF-1 alpha Antibody - BSA Free [NB100-479] -" data-caption="Chromatin Immunoprecipitation: HIF-1 alpha Antibody - BSA Free [NB100-479] - Hypoxia reduces inflammatory signaling pathways & NLRP3 expression & induces autophagy in IECs. a HT-29 cells were subjected to normoxia & hypoxia at the indicated times in the absence or presence of 10 µg/ml LPS. Autophagy was measured by variations in the ratio of LC3-II/LC3-I & the total amount of LC3 (LC3-I plus LC3-II) relative to beta-actin. Results are representative of two independent experiments. b, c & d HT-29 cells were subjected to normoxia & hypoxia for the indicated periods in the absence or presence of 10 µg/ml LPS, followed by transcript analysis. Statistical analysis was performed using one-way ANOVA followed by Tukey’s post-test. Results represent mean + s.e.m. of two independent experiments done in triplicate, *P " data-badges="" data-application="Chromatin Immunoprecipitation" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-479_rabbit-polyclonal-hif-1-alpha-antibody-310202415532013.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] -" data-caption="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] - Deletion of Hif1 alpha decreases primary tumor growth. (A) Wild-type (WT) or knockout (KO) cells (n = 50,000) were transplanted into FVB/Nj mice. All tumors were harvested at day 56 to evaluate tumor weight, volume & burden (percentage tumor weight/total body weight, BW) (n = 10 recipients/genotype, P < 0.05, unpaired Student's t-test). (B) The growth rate of WT & KO tumors following transplant of 50,000 cells. Best-fit curves were established based on a polynomial fit algorithm using GraphPad Prism 4.0 software. Data in (A) & (B) are representative of seven independent experiments (> 60 recipients/genotype). (C) When 500 WT & KO cells were implanted into the mammary fat pad, the median time until 50% of recipients developed tumors > 500 mm3 was 64 days for WT mice & 127 days for KO mice (n = 14 recipients/genotype, P < 0.001, logrank test). (D) Western blot for HIF-1 alpha in three independent tumors (500 to 750 mm3) per genotype. WT & KO cells that were cultured for 6 hours under hypoxic conditions served as positive & negative controls, respectively. CRM, cross-reactive material. MTEC, mammary tumor epithelial cell. (E) Mean fold change ± SEM in expression of HIF-1 targets in KO tumors as determined by qRT-PCR (n = 5 tumors/genotype). Decreased gene expression in KO tumors is presented as a negative fold-change relative to WT tumors. (F) An increase in Ki67+ cells in KO tumors is balanced by an increase in caspase 3-positive cells (n = 5 tumors/genotype, *P < 0.05, Student's t-test). Representative immunostaining images are shown in Additional file 2 Figure S4. Image collected & cropped by CiteAb from the following publication (http://breast-cancer-research.biomedcentral.com/articles/10.1186/bcr3087), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-479_rabbit-polyclonal-hif-1-alpha-antibody-310202415532077.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] -" data-caption="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] - Effect of HIF-1 alpha silencing on alpha-MSH-induced apoptosis in B16-F10 melanoma cells during hypoxia.a Cells were transfected with shLacZ or shHIF-1 alpha plasmids for 48 h before harvest. HIF-1 alpha shRNA reduced the basal HIF-1 alpha mRNA & protein expression levels. b, c Relative mRNA expression levels were analyzed by real-time PCR. Data are expressed as fold change compared with control (means ± SD of triplicate experiments). d Cell lysates were analyzed by immunoblot using the indicated antibodies. beta-Actin was used as an internal control for loading & transfer. e The population of apoptotic cells was analyzed by flow cytometry & qualified as mean ± SD from triplicate experiments. *p " data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-479_rabbit-polyclonal-hif-1-alpha-antibody-31020241682177.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Immunohistochemistry: HIF-1 alpha Antibody - BSA Free [NB100-479] -" data-caption="Immunohistochemistry: HIF-1 alpha Antibody - BSA Free [NB100-479] - Hif1 alpha expression in neonatal & adult testis.(A) Section of testis from 5-day old (P5) male new born pups showing Hif1 alpha expression in MVH+ gonocytes within the seminiferous tubules (top) & negative control images without primary antibodies (bottom). Scale bar: 50μm. (B) Western blot analysis of Hif1 alpha expression in P5 testes (left) compared to extract of the adult brain sub-ventricular zone (SVZ) (right). Loading control ( beta-Actin) is shown below. (C) Section of adult (3 month old) testis showing Hif1 alpha expression in spermatogonia. Scale bar: 30μm. (D) Western blot analysis of whole adult testis. HEK293 cells treated with DFX were used as a positive control & intestinal tissue was used as a negative control. Loading control ( beta-Actin) is shown below. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27148974), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Immunohistochemistry" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-479_rabbit-polyclonal-hif-1-alpha-antibody-310202415535154.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] -" data-caption="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] - Expression of hypoxia-inducible factor (HIF) proteins after bilateral renal ischemia or sham ischemia. Immunoblots for HIF-1 alpha (A–C) & HIF-2 alpha (D–F) of tissue extracts from the cortex & outer & inner medulla of the left kidneys of rats 24 h & 5 days following recovery from either sham ischemia (○) or bilateral renal ischemia (●); n = 6 per group. G: typical image of the gel following electrophoresis. H: typical image of the nitrocellulose membrane following transfer. Values are expressed as medians (25th percentile, 75th percentile). Paired comparisons were performed using the Mann-Whitney U-test. Because paired comparisons were made at two time points, P values were conservatively adjusted using the Dunn-Sidak method with k = 2. PTr, PT, & PTr*T are the outcomes of two-way analysis of variance on ranking with the factors treatment (Tr) & time (T). AU, arbitrary unit; I1, 24 h after ischemia; I5, 5 days after ischemia; S1, 24 h after sham ischemia; S5, 5 days after sham ischemia. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30110566), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-479_rabbit-polyclonal-hif-1-alpha-antibody-310202415541975.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] -" data-caption="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] - Hypoxia reduces inflammatory signaling pathways & NLRP3 expression & induces autophagy in IECs. a HT-29 cells were subjected to normoxia & hypoxia at the indicated times in the absence or presence of 10 µg/ml LPS. Autophagy was measured by variations in the ratio of LC3-II/LC3-I & the total amount of LC3 (LC3-I plus LC3-II) relative to beta-actin. Results are representative of two independent experiments. b, c & d HT-29 cells were subjected to normoxia & hypoxia for the indicated periods in the absence or presence of 10 µg/ml LPS, followed by transcript analysis. Statistical analysis was performed using one-way ANOVA followed by Tukey’s post-test. Results represent mean + s.e.m. of two independent experiments done in triplicate, *P " data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-479_rabbit-polyclonal-hif-1-alpha-antibody-310202415525255.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] -" data-caption="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] - Transient hyperoxia does not sensitize normal human astrocytes to radiation.Results are shown for the colony forming assays for all previously assayed cell lines & a normal human astrocyte cell line (Astro). Cells were continuously maintained under normoxic conditions (NOx) or exposed to 25 min of hyperoxia (50% O2) & then returned to normoxic conditions for 25 min (HyperOx) before being treated with a 5 Gy dose of radiation. To allow for ease of comparisons among cell types, raw values are expressed as a percentage of the corresponding cells type’s negative (non-irradiated) control & the means & SEMs are plotted. Each result represents three independent samples, plated in triplicate (*p" data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-479_rabbit-polyclonal-hif-1-alpha-antibody-310202415532080.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] -" data-caption="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-479] - YC-1 attenuates pro-inflammatory differentiation of macrophages. (a,b) qRT-PCR analyses, revealing mRNA expression of the pro-inflammatory M1-differentiation markers NOS2 (a) & IL-6 (b) in murine J774A.1 macrophages under normoxia (left bars) & hypoxia (right bars). LPS exposure was carried out to induce M1-polarization in presence of YC-1 or vehicle control (DMSO; **P ≤ 0.01, n = 9). (c,d) Western blots of nuclear extracts, revealing protein levels of HIF-1 alpha (c) & HIF-2 alpha (d) in J774.A1 macrophages treated with vehicle (DMSO) or YC-1 under normoxic (left) or hypoxic (right) culture conditions. Simultaneous LPS-treatment was performed to induce pro-inflammatory activation. Note LPS-induced stabilization of HIF-1 alpha- (c), but not HIF-2 alpha-levels (d), & reversal of LPS-induced HIF-1 alpha-stabilization by YC-1 (c). Histone H3 was used as a loading control. (e,f) qRT-PCR analyses, revealing mRNA expression of the pro-inflammatory M1-differentiation markers NOS2 (e) & IL-6 (f) in BMDMs under normoxia (left bars) & hypoxia (right bars). LPS exposure was carried out to induce M1-polarization in presence of YC-1 or vehicle control (DMSO; ***P ≤ 0.001, **P ≤ 0.01, n = 6). Image collected & cropped by CiteAb from the following publication (https://www.nature.com/articles/s41598-017-13638-z), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-479" data-width="250" data-height="250" class="none"></i> </div> </div> </div> </div> <div class="atc-block-search atc-block-collapsible" data-type="search" data-blockid="ProductCart-NB100-479" > <form action="/commerce/addtocart" method="post" class="px-3 pt-3 add-to-cart-block" data-productcode="NB100-479"> <div class="commerce-atc-full-header atc-header col-four row border-bottom pb-1 m-0"> <div class="commerce-atc-catnum atc-head col-4 col-md-4 font-weight-bold">Catalog #</div> <div class="commerce-atc-availability atc-head col-md-3 font-weight-bold d-none d-md-block">Availability </div> <div class="commerce-atc-price atc-head col-5 col-md-3 font-weight-bold">Size / Price</div> <div class="commerce-atc-quantity atc-head col-3 col-md-2 text-right font-weight-bold">Qty</div> </div> <div class="spinner-border spinner-border-sm mt-3 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Antibody </h4> </a> </div> <div class="search_result_brand_logo col-md-3 mb-1 d-flex justify-content-xl-end product-brand-logo"> <img alt="Novus Biologicals, a Bio-Techne brand" src="https://resources.bio-techne.com/bio-techne-assets/images/logos/bio-techne-novus-biologicals.svg" class="d-flex w-75"> </div> </div> <div class="row"> <div class="col-md-9"> <div class="mb-1 d-inline-flex"> <div class="align-middle my-1 mr-3 d-block d-md-inline"> Catalog #: <a href="/p/antibodies/hif-1-alpha-antibody_nb100-449" title="HIF-1 alpha Antibody"> NB100-449 </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold"> <a href="/p/antibodies/hif-1-alpha-antibody_nb100-449#reviews" class="searchImage_star4"> (29) </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold"> <a href="/p/antibodies/hif-1-alpha-antibody_nb100-449#citations"><span class="icon-icon-product-citations"></span>Citations (310)</a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold text-nowrap"> <a href="/p/antibodies/hif-1-alpha-antibody_nb100-449#product-documents"><span class="icon-icon-pdf text-error-red"></span> Product Documents</a> </div> <div class="d-none d-md-inline my-1"> <a href="#" class="search_result_images"><span class="icon-icon-image-camera-plus"></span> Images (55)</a> </div> </div> <div class="d-none d-md-block"> <span class="search_result_applications"></span><span class="applications_title"><strong>Applications:</strong></span> IHC, WB, ELISA, ICC/IF, Flow, ChIP, Func, IP, KD, KO, Simple Western </div> <div class="d-none d-md-block"> <span class="species_title"><strong>Reactivity:</strong></span> Human, Mouse, Rat, Canine, Monkey, +4 More </div> </div> <div class="col-md-3"> <button class="py-1 btn-default btn-block atc-view-more collapsed" data-toggle="collapse" href="#collapseButton-2" role="button" aria-expanded="false" aria-controls="collapseButton">View More</button> </div> </div> <div class="commerce-atc-search pt-3 collapse" id="collapseButton-2"> <div class="row"> <div class="search_result_description col-xs-12 col-md-9"> <table class="tablesaw tablesaw-stack mb-2"> <tbody> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Reactivity:</strong></td> <td class="pb-1 w-75 text-break"> Human, Mouse, Rat, Canine, Monkey, +4 More </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Details:</strong></td> <td class="pb-1 w-75 text-break"> Rabbit IgG Polyclonal </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Applications:</strong></td> <td class="pb-1 w-75 text-break"> IHC, WB, ELISA, ICC/IF, Flow, +6 More </td> </tr> </tbody> </table> <div class="compare_tool_select form-inline mb-2" data-sku="nb100-449"></div> </div> <div class="search_result_image_wrapper col-xs-12 col-md-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="novus"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src=https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-Knockdown-Validated-NB100-449-img0050.jpg data-srcset=https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-Knockdown-Validated-NB100-449-img0050.jpg alt="Knockdown Validated: HIF-1 alpha Antibody [NB100-449]" title="Knockdown Validated: HIF-1 alpha Antibody [NB100-449]" data-caption="Knockdown Validated: HIF-1 alpha Antibody [NB100-449] - Morphological alterations induced by DMOG are HIF-1alpha dependent. glEND.2 cells were stably transfected with shGFP, shHIF-1alpha or shHIF-2alpha. The clones were cultured under normoxic (N) or hypoxic (H, 1% O2) conditions, or treated with DMOG for 6 h. 30 ug protein of cellular protein were loaded for the detection of HIF-1alpha, and nuclear extracts (20 ug protein) were used for the detection of HIF-2alpha. Image collected and cropped by CiteAb from the following publication (https://biosignaling.biomedcentral.com/articles/10.1186/1478-811X-11-80) licensed under a CC-BY license." data-badges="<span class="knockout-validation"></span>" data-application="Knockdown Validated" data-header="HIF-1 alpha Antibody, Catalog # NB100-449" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> <span class="image-count-text align-middle">(55)</span> </div> </div> </div> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-Western-Blot-NB100-449-img0015.jpg" data-alt="Western Blot: HIF-1 alpha Antibody [NB100-449]" title="Western Blot: HIF-1 alpha Antibody [NB100-449]" data-caption="Western Blot: HIF-1 alpha Antibody [NB100-449] - HIF-1 alpha induction on Caki-1 cell lysate using CoCl2. Image from verified customer review." data-badges="<span class="biological-validation"></span>" data-application="Western Blot" data-header="HIF-1 alpha Antibody, Catalog # NB100-449" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-Simple-Western-NB100-449-img0032.jpg" data-alt="Simple Western: HIF-1 alpha Antibody [NB100-449]" title="Simple Western: HIF-1 alpha Antibody [NB100-449]" data-caption="Simple Western: HIF-1 alpha Antibody [NB100-449] - Simple Western lane view shows a specific band for HIF-1 alpha in 0.5 mg/ml of hypoxic HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system." data-badges="" data-application="Simple Western" data-header="HIF-1 alpha Antibody, Catalog # NB100-449" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-Immunohistochemistry-NB100-449-img0034.jpg" data-alt="Immunohistochemistry: HIF-1 alpha Antibody [NB100-449]" title="Immunohistochemistry: HIF-1 alpha Antibody [NB100-449]" data-caption="Immunohistochemistry: HIF-1 alpha Antibody [NB100-449] - Mouse Brain, Neurons 40X" data-badges="" data-application="Immunohistochemistry" data-header="HIF-1 alpha Antibody, Catalog # NB100-449" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-Immunohistochemistry-NB100-449-img0047.jpg" data-alt="Immunohistochemistry: HIF-1 alpha Antibody [NB100-449]" title="Immunohistochemistry: HIF-1 alpha Antibody [NB100-449]" data-caption="Immunohistochemistry: HIF-1 alpha Antibody [NB100-449] - Detection of human HIF1-alpha by immunohistochemistry. Sample: FFPE section of renal cell carcinoma. Antibody: Affinity purified rabbit anti-HIF1-alpha antibody (NB110-58773). Detection: DAB" data-badges="" data-application="Immunohistochemistry" data-header="HIF-1 alpha Antibody, Catalog # NB100-449" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-Western-Blot-NB100-449-img0038.jpg" data-alt="Western Blot: HIF-1 alpha Antibody [NB100-449]" title="Western Blot: HIF-1 alpha Antibody [NB100-449]" data-caption="Western Blot: HIF-1 alpha Antibody [NB100-449] - Detection of Human HIF-1 alpha by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells that were either treated with cobalt chloride (+; 200 uM) or mock treated (-). Antibodies: Affinity purified rabbit anti-HIF1 alpha antibody used for WB at 0.1 ug/mL(A) and 1 ug/mL (B) and used for IP at 3 ug/mg lysate. HIF-1 alpha was also immunoprecipitated by a previous lot of this antibody. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B)." data-badges="<span class="biological-validation"></span>" data-application="Western Blot" data-header="HIF-1 alpha Antibody, Catalog # NB100-449" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-Western-Blot-NB100-449-img0053.jpg" data-alt="Western Blot: HIF-1 alpha Antibody [NB100-449]" title="Western Blot: HIF-1 alpha Antibody [NB100-449]" data-caption="Western Blot: HIF-1 alpha Antibody [NB100-449] - Blot showing the effect of hypoxia on the protein expression levels of the purine biosynthetic enzymes. HIF-1 alpha is stabilized in hypoxia as expected, and no significant increase in the purine enzymes was detected between normoxic (21% oxygen) and hypoxic (1% oxygen) growth conditions. The positions of molecular markers surrounding each band of interest are shown for each blot. ADSL (NBP2-03107), ATIC (NBP2-01941), FGAMS (NBP1-84691), GART (H00002618-M01), HIF-1a (NB100-449), PAICS (NBP2-02817), PPAT (NBP2-02056). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32439803/) licensed under a CC-BY license." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody, Catalog # NB100-449" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NB100-449-img0039.jpg" data-alt="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody [NB100-449]" title="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody [NB100-449]" data-caption="Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody [NB100-449] - Murine primary bone marrow derived macrophages stained with HIF1-alpha antibody (red). Nuclei were counterstained with DAPI (blue). Image from verified customer review." data-badges="" data-application="Immunocytochemistry/ Immunofluorescence" data-header="HIF-1 alpha Antibody, Catalog # NB100-449" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-Immunohistochemistry-Paraffin-NB100-449-img0052.jpg" data-alt="Immunohistochemistry-Paraffin: HIF-1 alpha Antibody [NB100-449]" title="Immunohistochemistry-Paraffin: HIF-1 alpha Antibody [NB100-449]" data-caption="Immunohistochemistry-Paraffin: HIF-1 alpha Antibody [NB100-449] - Analysis of microvascular density and HIF-1alpha activity. Low, homogenous expression of HIF-1alpha was detected in the tumors of NNK treated mice (40X, scale bar = 25 um). Image collected and cropped by CiteAb from the following publication (https://molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-11-4), licensed under a CC-BY license." data-badges="<span class="biological-validation"></span>" data-application="Immunohistochemistry-Paraffin" data-header="HIF-1 alpha Antibody, Catalog # NB100-449" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-Flow-Cytometry-NB100-449-img0035.jpg" data-alt="Flow Cytometry: HIF-1 alpha Antibody [NB100-449]" title="Flow Cytometry: HIF-1 alpha Antibody [NB100-449]" data-caption="Flow Cytometry: HIF-1 alpha Antibody [NB100-449] - HeLa cells were treated for 15 hrs with 200 uM CoCl2, fixed in PFA, and permeabilized in 90% MeOH. 1 X 10^6 cells were stained with 0.125 ug anti-HIF-1 alpha and secondary FITC-conjugated goat anti-rabbit (in a 150 uL reaction). Black: treated, anti-KLH control IgG; Red: untreated, anti-HIF-1 alpha; Blue: treated, anti-HIF-1 alpha." data-badges="<span class="biological-validation"></span>" data-application="Flow Cytometry" data-header="HIF-1 alpha Antibody, Catalog # NB100-449" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-Western-Blot-NB100-449-img0033.jpg" data-alt="Western Blot: HIF-1 alpha Antibody [NB100-449]" title="Western Blot: HIF-1 alpha Antibody [NB100-449]" data-caption="Western Blot: HIF-1 alpha Antibody [NB100-449] - Analysis of HIF-1 alpha in human myeloma cell lysate using anti-HIF-1 alpha. Cells were untreated or treated with IGF-1, IL-6 or CoCl2. Image from verified customer review." data-badges="<span class="biological-validation"></span>" data-application="Western Blot" data-header="HIF-1 alpha Antibody, Catalog # NB100-449" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-Western-Blot-NB100-449-img0036.jpg" data-alt="Western Blot: HIF-1 alpha Antibody [NB100-449]" title="Western Blot: HIF-1 alpha Antibody [NB100-449]" data-caption="Western Blot: HIF-1 alpha Antibody [NB100-449] - Detection of HIF-1 alpha in a hypoxic sample. Lane 1: CoCl2 treated Cos-7 nuclear extract (hypoxic). Lane 2: Untreated Cos-7 nuclear extract (normoxic)." data-badges="<span class="biological-validation"></span>" data-application="Western Blot" data-header="HIF-1 alpha Antibody, Catalog # NB100-449" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-Western-Blot-NB100-449-img0037.jpg" data-alt="Western Blot: HIF-1 alpha Antibody [NB100-449]" title="Western Blot: HIF-1 alpha Antibody [NB100-449]" data-caption="Western Blot: HIF-1 alpha Antibody [NB100-449] - Detection of mouse HIF-1 alpha on hypoxia treated MEFs" data-badges="<span class="biological-validation"></span>" data-application="Western Blot" data-header="HIF-1 alpha Antibody, Catalog # NB100-449" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-Western-Blot-NB100-449-img0042.jpg" data-alt="Western Blot: HIF-1 alpha Antibody [NB100-449]" title="Western Blot: HIF-1 alpha Antibody [NB100-449]" data-caption="Western Blot: HIF-1 alpha Antibody [NB100-449] - Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells that were treated with cobalt chloride (+; 200 uM) or mock treated (-). Antibodies: Affinity purified rabbit anti-HIF1 alpha antibody NB100-449 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with exposure times of 30 seconds." data-badges="<span class="biological-validation"></span>" data-application="Western Blot" data-header="HIF-1 alpha Antibody, Catalog # NB100-449" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-Western-Blot-NB100-449-img0043.jpg" data-alt="Western Blot: HIF-1 alpha Antibody [NB100-449]" title="Western Blot: HIF-1 alpha Antibody [NB100-449]" data-caption="Western Blot: HIF-1 alpha Antibody [NB100-449] - BMDM were seeded at 0.5x10^6 overnight. Cells were treated with 10 ng/ml LPS for 24 hrs. Image from verified customer review." data-badges="<span class="biological-validation"></span>" data-application="Western Blot" data-header="HIF-1 alpha Antibody, Catalog # NB100-449" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-Western-Blot-NB100-449-img0044.jpg" data-alt="Western Blot: HIF-1 alpha Antibody [NB100-449]" title="Western Blot: HIF-1 alpha Antibody [NB100-449]" data-caption="Western Blot: HIF-1 alpha Antibody [NB100-449] - Analysis of total protein using anti-HIF-1 alpha, -CAIX, -ISCU antibodies and VDACs antibody (PMID: 29596470)." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody, Catalog # NB100-449" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-Western-Blot-NB100-449-img0045.jpg" data-alt="Western Blot: HIF-1 alpha Antibody [NB100-449]" title="Western Blot: HIF-1 alpha Antibody [NB100-449]" data-caption="Western Blot: HIF-1 alpha Antibody [NB100-449] - Analysis of total protein using anti-HIF-1 alpha, -ISCU, -FXN antibodies. CoCl2 was used to treat or not HeLa cells for 2 days (PMID: 29596470)." data-badges="<span class="biological-validation"></span>" data-application="Western Blot" data-header="HIF-1 alpha Antibody, Catalog # NB100-449" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-Western-Blot-NB100-449-img0046.jpg" data-alt="Western Blot: HIF-1 alpha Antibody [NB100-449]" title="Western Blot: HIF-1 alpha Antibody [NB100-449]" data-caption="Western Blot: HIF-1 alpha Antibody [NB100-449] - Detection of human HIF1 alpha by western blot. Samples: Whole cell lysate (15 and 50 ug) from HEK293T cells that were either treated with 200 uM cobalt chloride (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-HIF1 alpha antibody NB100-449 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with exposure times of 3 minutes." data-badges="<span class="biological-validation"></span>" data-application="Western Blot" data-header="HIF-1 alpha Antibody, Catalog # NB100-449" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-Western-Blot-NB100-449-img0051.jpg" data-alt="Western Blot: HIF-1 alpha Antibody [NB100-449]" title="Western Blot: HIF-1 alpha Antibody [NB100-449]" data-caption="Western Blot: HIF-1 alpha Antibody [NB100-449] - 3T3-L1 mouse embryonic fibroblast adipose-like cell line. Antibody at 1:2000. WB image submitted by a verified customer review." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody, Catalog # NB100-449" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NB100-449-img0006.jpg" data-alt="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody [NB100-449]" title="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody [NB100-449]" data-caption="Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody [NB100-449] - Formaldehyde-fixed asynchronous HeLa cells." data-badges="<span class="biological-validation"></span>" data-application="Immunocytochemistry/ Immunofluorescence" data-header="HIF-1 alpha Antibody, Catalog # NB100-449" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-Immunoprecipitation-NB100-449-img0005.jpg" data-alt="Immunoprecipitation: HIF-1 alpha Antibody [NB100-449]" title="Immunoprecipitation: HIF-1 alpha Antibody [NB100-449]" data-caption="Immunoprecipitation: HIF-1 alpha Antibody [NB100-449] - HIF-1 alpha analysis in HEK293 cells. Image from verified customer review." data-badges="" data-application="Immunoprecipitation" data-header="HIF-1 alpha Antibody, Catalog # NB100-449" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-Immunoprecipitation-NB100-449-img0048.jpg" data-alt="Immunoprecipitation: HIF-1 alpha Antibody [NB100-449]" title="Immunoprecipitation: HIF-1 alpha Antibody [NB100-449]" data-caption="Immunoprecipitation: HIF-1 alpha Antibody [NB100-449] - Detection of human HIF1 alpha by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HEK293T cells that were treated with 200 uM cobalt chloride. Antibodies: Affinity purified rabbit anti-HIF1 alpha antibody NB100-449 used for WB at 1 ug/ml and for IP at 6 ug/mg lysate. Detection: Chemiluminescence with exposure times of 3 minutes." data-badges="<span class="biological-validation"></span>" data-application="Immunoprecipitation" data-header="HIF-1 alpha Antibody, Catalog # NB100-449" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-449_rabbit-polyclonal-hif-1-alpha-antibody-271220231253349.jpg" data-alt="HIF-1 alpha Antibody" title="Western Blot: HIF-1 alpha Antibody [NB100-449] -" data-caption=" beta-Glucan size affects the requirement for reactive oxygen species (ROS) in IL-1 beta induction.(B) Human mDCs from healthy donors or chronic granulomatous disease (CGD) patients were stimulated with curdlan or glucan-mp for 8 h. HIF-1 alpha and pro-IL-1 beta protein expression measured by immunoblot (n = 2 donors)." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody, Catalog # NB100-449" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-449_rabbit-polyclonal-hif-1-alpha-antibody-western-blot-22202414855..jpg" data-alt="HIF-1 alpha Antibody" title="Western Blot: Rabbit Polyclonal HIF-1 alpha Antibody -" data-caption="Western Blot: Rabbit Polyclonal HIF-1 alpha Antibody - Analysis of HIF-1 alpha antibody on mouse liver nuclear extracts. Image from a verified customer review." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody, Catalog # NB100-449" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-449_rabbit-polyclonal-hif-1-alpha-antibody-210202423452449.jpg" data-alt="HIF-1 alpha Antibody" title="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody [NB100-449] -" data-caption="HIF-1 alpha/2 alpha expression in myeloid-specific KO mice targeting the HIF pathway. (A) Images of the colon of wild-type (WT), myeloid-specific Hif-1a KO (hMRP8 Hif-1a KO) or von Hippel Lindau (Vhl) KO (hMRP8 Vhl KO) mice, immunostained for MRP8 (green) & the DNA-binding regions of Hif-1a mRNA (red). Mice were fed with 5% DSS for 4 days prior to immunostaining analyses. Note that there were no MRP8-positive cells that were positive for Hif-1a mRNA in hMRP8 Hif-1a KO (middle column) mice, but we observed many cells that were double positive for MRP8 & Hif-1a mRNA in hMRP8 Vhl KO mice (right column). (B) Images of the colon of hMRP8 Vhl KO mice fed with 5% DSS as in A, immunostained for MRP8 (green) & HIF-1 alpha (red, upper row) or HIF-2 alpha (red, bottom row). DAPI-stained nuclei are shown in blue. White boxes in A & B indicate the regions magnified in the lower or right images, respectively. Yellow arrowheads in A & B indicate cells positive for both markers. Scale bars: 100 μm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29967068), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Immunocytochemistry/ Immunofluorescence" data-header="HIF-1 alpha Antibody, Catalog # NB100-449" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-449_rabbit-polyclonal-hif-1-alpha-antibody-310202415284545.jpg" data-alt="HIF-1 alpha Antibody" title="Immunohistochemistry: HIF-1 alpha Antibody [NB100-449] -" data-caption="Immunohistochemistry: HIF-1 alpha Antibody [NB100-449] - Analysis of microvascular density & HIF-1 alpha activity. Microvascular density of pulmonary tumors was significantly higher in hyperplastic (B) & tumoral lesions (D) of NNK/NTHi treated mice compared to the NNK treated mice (A, & C) detected by CD105 immunostaining (10X, scale bar = 100 μm). HIF-1 alpha immunostaining after NNK/NTHi combined treatment showed hot-spots of high stromal expression in tumors (F), & high expression in perivascular-peribronchiolar lymphocytes (H). In contrast, low, homogenous expression of HIF-1 alpha was detected in the tumors (E) & perivascular-peribronchiolar lymphocytes (G) of NNK treated mice (40X, scale bar = 25 μm). Image collected & cropped by CiteAb from the following publication (https://molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-11-4), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Immunohistochemistry" data-header="HIF-1 alpha Antibody, Catalog # NB100-449" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-449_rabbit-polyclonal-hif-1-alpha-antibody-310202415304212.jpg" data-alt="HIF-1 alpha Antibody" title="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody [NB100-449] -" data-caption="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody [NB100-449] - Chronic ethanol feeding enhances tumorigenesis markers during AOM/DSS-induced tumorigenesis. AOM/DSS-induced colonic tumorigenesis was induced with (EF) or without (PF) 4 % ethanol feeding as described in Methods section. Cryosections of distal colon were stained for VEGF (a) pSmad (b), or HIF1 alpha (c), & co-stained for F-actin & nucleus. Images presented are representative of n of 5 for each group Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26951793), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Immunocytochemistry/ Immunofluorescence" data-header="HIF-1 alpha Antibody, Catalog # NB100-449" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-449_rabbit-polyclonal-hif-1-alpha-antibody-310202415363719.jpg" data-alt="HIF-1 alpha Antibody" title="Immunohistochemistry-Paraffin: HIF-1 alpha Antibody [NB100-449] -" data-caption="Immunohistochemistry-Paraffin: HIF-1 alpha Antibody [NB100-449] - Treatment with HBOT or PFD decreased HIF-1 alpha expression in the AD lesions.IHC for HIF-1 alpha (A). Scale bar is 100 µm. HIF-1 alpha+ area was measured in five fields chosen at random by using image analysis software (B). RT-PCR for HIF-1 alpha (C). NT, not treated. Data are expressed as the mean ± SD (n = 12). *P" data-badges="" data-application="Immunohistochemistry-Paraffin" data-header="HIF-1 alpha Antibody, Catalog # NB100-449" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-449_rabbit-polyclonal-hif-1-alpha-antibody-31020241534344.jpg" data-alt="HIF-1 alpha Antibody" title="Immunohistochemistry-Paraffin: HIF-1 alpha Antibody [NB100-449] -" data-caption="Immunohistochemistry-Paraffin: HIF-1 alpha Antibody [NB100-449] - Elevated PIK3/AKT/HIF-1 alpha pathways in KL SqCC tumours.(a,b) Analysis of TCGA gene expression (normalized TPM) & PIK3CA (a) & PTEN (b) genomic copy number alteration profiles. Each dot represents one SqCC patient (n=501). Boxes represent the interquartile range & whiskers are drawn to the minimum & maximum. Kruskal–Wallis non-parametric ANOVA, ****P" data-badges="" data-application="Immunohistochemistry-Paraffin" data-header="HIF-1 alpha Antibody, Catalog # NB100-449" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-449_rabbit-polyclonal-hif-1-alpha-antibody-310202415384135.jpg" data-alt="HIF-1 alpha Antibody" title="Immunohistochemistry: HIF-1 alpha Antibody [NB100-449] -" data-caption="Immunohistochemistry: HIF-1 alpha Antibody [NB100-449] - Analysis of microvascular density & HIF-1 alpha activity. Microvascular density of pulmonary tumors was significantly higher in hyperplastic (B) & tumoral lesions (D) of NNK/NTHi treated mice compared to the NNK treated mice (A, & C) detected by CD105 immunostaining (10X, scale bar = 100 μm). HIF-1 alpha immunostaining after NNK/NTHi combined treatment showed hot-spots of high stromal expression in tumors (F), & high expression in perivascular-peribronchiolar lymphocytes (H). In contrast, low, homogenous expression of HIF-1 alpha was detected in the tumors (E) & perivascular-peribronchiolar lymphocytes (G) of NNK treated mice (40X, scale bar = 25 μm). Image collected & cropped by CiteAb from the following publication (https://molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-11-4), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Immunohistochemistry" data-header="HIF-1 alpha Antibody, Catalog # NB100-449" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-449_rabbit-polyclonal-hif-1-alpha-antibody-310202415293325.jpg" data-alt="HIF-1 alpha Antibody" title="Immunohistochemistry: HIF-1 alpha Antibody [NB100-449] -" data-caption="Immunohistochemistry: HIF-1 alpha Antibody [NB100-449] - Immunolocalization of HIF1A in limbs.Immunoreactivity was detected in control limbs in the apical ectodermal ridge & interdigital regions (I.R.). The mesenchymal condensations/developing cartilaginous anlagen (Digits) were not immunoreactive. 4-OOHCPA treatment resulted in a concentration- & time-dependent increase in HIF1A immunoreactivity in this area in the 3 & 6 h exposure groups; staining was diminished by 24 h. Four separate replicates were done. Image collected & cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0051937), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Immunohistochemistry" data-header="HIF-1 alpha Antibody, Catalog # NB100-449" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-449_rabbit-polyclonal-hif-1-alpha-antibody-310202415394514.jpg" data-alt="HIF-1 alpha Antibody" title="Western Blot: HIF-1 alpha Antibody [NB100-449] -" data-caption="Western Blot: HIF-1 alpha Antibody [NB100-449] - Effects of cell stress on VEGF-A & HIF1 alpha expression in Müller cells & Y79 photoreceptors. (A) VEGF-A was measured by ELISA using conditioned media collected from MIO-M1 & Y79 cells, n = 4–5/group in Müller cells & n = 8/group in Y79 photoreceptors, ** p < 0.01; (B) Western blots for HIF1 alpha using cellular proteins, n = 4/group, ** p < 0.01. For MIO-M1 Müller cells: Grey bars: 5 mM glucose. Black bars: 25 mM glucose. For Y79 photoreceptors: Grey bars: 11 mM glucose. Black bars: 25 mM glucose. NS, not significant; LG, low glucose; HG, high glucose. Image collected & cropped by CiteAb from the following publication (http://www.mdpi.com/1422-0067/18/3/533), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody, Catalog # NB100-449" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-449_rabbit-polyclonal-hif-1-alpha-antibody-310202415291950.jpg" data-alt="HIF-1 alpha Antibody" title="Immunohistochemistry: HIF-1 alpha Antibody [NB100-449] -" data-caption="Immunohistochemistry: HIF-1 alpha Antibody [NB100-449] - Analysis of microvascular density & HIF-1 alpha activity. Microvascular density of pulmonary tumors was significantly higher in hyperplastic (B) & tumoral lesions (D) of NNK/NTHi treated mice compared to the NNK treated mice (A, & C) detected by CD105 immunostaining (10X, scale bar = 100 μm). HIF-1 alpha immunostaining after NNK/NTHi combined treatment showed hot-spots of high stromal expression in tumors (F), & high expression in perivascular-peribronchiolar lymphocytes (H). In contrast, low, homogenous expression of HIF-1 alpha was detected in the tumors (E) & perivascular-peribronchiolar lymphocytes (G) of NNK treated mice (40X, scale bar = 25 μm). Image collected & cropped by CiteAb from the following publication (https://molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-11-4), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Immunohistochemistry" data-header="HIF-1 alpha Antibody, Catalog # NB100-449" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-449_rabbit-polyclonal-hif-1-alpha-antibody-310202415304256.jpg" data-alt="HIF-1 alpha Antibody" title="Western Blot: HIF-1 alpha Antibody [NB100-449] -" data-caption="Western Blot: HIF-1 alpha Antibody [NB100-449] - Phenylhydrazine administration results in hypoxia in retinal vessels & in HIF-1 alpha & HIF-2 alpha stabilization in peripheral blood leukocytes.(A) 3-dimensional reconstructed imaging of the superficial vascular plexus of flat mounted retinae of mice (steady state, treated with PHZ & treated with PHZ & peak EIU induction) stained with DAPI, hypoxyprobe & Isolectin B4. (B) Representative analysis of HIFs by western blot: 50ug of protein from total cell lysate of blood leukocytes isolated from mice treated with PHZ compared to untreated; (C) relative densitometry quantification of HIF western blots from PBMC showing mean, n = 3, error bar showing SD, unpaired t test, ***P " data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody, Catalog # NB100-449" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-449_rabbit-polyclonal-hif-1-alpha-antibody-31020241553383.jpg" data-alt="HIF-1 alpha Antibody" title="Immunohistochemistry-Frozen: HIF-1 alpha Antibody [NB100-449] -" data-caption="Immunohistochemistry-Frozen: HIF-1 alpha Antibody [NB100-449] - Ethanol elevates tumorigenic markers & nuclear localization of HIF1 alpha in colonic crypts during AOM/DSS-induced tumorigenesis: AOM/DSS-induced colonic tumorigenesis was induced with or without 4 % ethanol feeding as described in Methods section. a & b Colonic mucosal extracts were immunoblotted for pSmad & VEGF (a), & the band density evaluated using Image J software (b). Values are mean ± SE (n = 3). Asterisks indicate the values that are significantly different (p " data-badges="" data-application="Immunohistochemistry-Frozen" data-header="HIF-1 alpha Antibody, Catalog # NB100-449" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-449_rabbit-polyclonal-hif-1-alpha-antibody-310202415532021.jpg" data-alt="HIF-1 alpha Antibody" title="Western Blot: HIF-1 alpha Antibody [NB100-449] -" data-caption="Western Blot: HIF-1 alpha Antibody [NB100-449] - Dectin-1-induced NADPH oxidase-derived ROS is required for augmented IL-1 beta during TSLPR inhibition. (A) Human mDC derived from healthy donors (HD) or CGD patients were stimulated with SC glucan for 8 h (n = 2 representative donors presented, three separate experiments performed). Pro-IL-1 beta, IL-1 beta, HIF-1 alpha, phospho-AMPK, AMPK & beta-actin were measured by immunoblot. (B–E) Densitometry of cumulative data was performed using Image Studio Lite software with pro-IL-1 beta, IL-1 beta & HIF-1 alpha normalized to beta-actin & phospho-AMPK normalized to total AMPK. Data is reported as percentage of maximal signal observed within each donor (n = 3 independent donors, presented as pooled data). (F–H) Human mDC derived from HD or CGD patients were stimulated with SC glucan, CA glucan or heat killed C. albicans hyphae with anti-TSLPR or IgG isotype control antibodies for 24 h (n = 3 independent donors, presented as pooled data). IL-1 beta was measured in 24-h cell culture supernatants by ELISA. Cumulative data displayed as mean +SEM. Statistical analysis calculated using one-way ANOVA with Bonferroni post-tests (***p = 0.001,*p = 0.05). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31139177), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody, Catalog # NB100-449" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-449_rabbit-polyclonal-hif-1-alpha-antibody-310202415525284.jpg" data-alt="HIF-1 alpha Antibody" title="Western Blot: HIF-1 alpha Antibody [NB100-449] -" data-caption="Western Blot: HIF-1 alpha Antibody [NB100-449] - Reactive oxygen species production & expression of stabilized HIF1 alpha & AMPK subunit alpha2 & in H1299 & H1299r cells(A) H1299, H1299r, P31 & P31r cell were seeded at a density of 250,000 cells/well & left for 24 h. The cells were then stained with 10 μmol/L dichloro-dihydro-fluorescein diacetate (DCFH-DA). Fluorescence was analysed for 10,000 events. Whole cell lysates were prepared from confluent cultures of H1299, H1299r cells. Proteins (90 μg) were resolved in 10% SDS-PAGE gels & transferred to a PVDF membrane. (B) Representative blot of HIF1 alpha protein expression. (C) Representative blot of AMPK subunit alpha2 protein expression from four independent experiments. (D) Bar chart of AMPK subunit alpha2 mean for four separate measurements. Statistical analyses was carried out using the student t-test. ** = p" data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody, Catalog # NB100-449" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-449_rabbit-polyclonal-hif-1-alpha-antibody-31020241612685.jpg" data-alt="HIF-1 alpha Antibody" title="Western Blot: HIF-1 alpha Antibody [NB100-449] -" data-caption="Western Blot: HIF-1 alpha Antibody [NB100-449] - Pyruvate kinase isoform M2 (PKM2) & hypoxia-inducible factor 1 alpha (Hif-1 alpha) bind directly to the promoter region of PDL1 in primary murine BMDM cells. BMDM cells were treated with TEPP-46 at 50 µM for 1 h prior to stimulation with LPS (100 ng/ml, 24 h) (A). Binding of PKM2 (left) or Hif-1 alpha (right) to HRE1 or HRE4 of the PD-L1 promoter was detected by incubating cell lysates with biotinylated oligonucleotides spanning the relevant HRE promoter region. Protein–oligonucleotide complexes were isolated using streptavidin agarose beads, & proteins were detected by western blotting. Representative of n = 3. Chromatin immunoprecipitation (ChIP)-PCR (B) using PKM2 & HIF-1 alpha antibodies & primers specific for three promoter regions of PD-L1 (HRE1, 2–3, & 4) & a known Hif-1 alpha binding region of vascular endothelial growth factor as a positive control showing binding of Hif-1 alpha (left) & PKM2 (right) to the PD-L1 promoter in LPS-treated BMDMs (100 ng/ml, 24 h). (C) Sequential ChIP assays measuring simultaneous endogenous binding of PKM2 & Hif-1 alpha to chromatin in response to LPS (100 ng/ml, 24 h) ±TEPP-46 (pretreatment using 50 µM, 60 min). ChIP data are calculated as percent of input, error bar represents mean ± SEM, & statistics are performed as two-tailed unpaired t-test *P " data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody, Catalog # NB100-449" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-449_rabbit-polyclonal-hif-1-alpha-antibody-31020241552391.jpg" data-alt="HIF-1 alpha Antibody" title="Western Blot: HIF-1 alpha Antibody [NB100-449] -" data-caption="Western Blot: HIF-1 alpha Antibody [NB100-449] - Changes in Hepatic Oxygen Saturation & Hypoxia-related Protein Expression. (A) In the representative case, the hepatic oxygen saturation in the experimental group was lower than that in the control group at four, six, & eight weeks (51.6 vs. 54.5%, 48.4 vs. 53.9%, 32.3 vs. 43.2%, & 31.2 vs. 42.9%). (B) There was significant decrease of oxygen saturation of liver parenchyma in the experimental group compared to those of control group (45.2 vs. 51.9%, 43.0 vs. 49.0%, & 31.3 vs. 49.7%) at the four, six, & eight weeks, respectively (P " data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody, Catalog # NB100-449" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-449_rabbit-polyclonal-hif-1-alpha-antibody-31020241554543.jpg" data-alt="HIF-1 alpha Antibody" title="Western Blot: HIF-1 alpha Antibody [NB100-449] -" data-caption="Western Blot: HIF-1 alpha Antibody [NB100-449] - BCAT1 expression & inhibition in human macrophages.(a) RNA-sequencing in human monocyte-derived macrophages (hMDMs) shows mRNA copies of BCAT enzymes in normalized counts. n=14 healthy donor hMDMs were used for RNA-seq. (b) Chemical structure of ERG240 & leucine. (c) Enzymatic activity of recombinant human BCAT1 & BCAT2 in presence of ERG240. (d) Relative expression of IRG1, HIF1A & IL1B measured by qRT-PCR in control, ERG240-treated (20 mM, 3 h), LPS treated (100 ng ml−1, 3 h), & LPS+ERG240 treated (LPS, 100 ng ml−1; ERG240, 20 mM for 3 h) hMDMs. ns, non-significant. All expression values are normalized to those obtained for HPRT gene expression. n=3 healthy donor hMDMs were used in each group. (e) Western blot analysis of IRG1, IL-1 beta, HIF-1 alpha & beta-actin (ACTB) in control (untreated), ERG240-treated (20 mM, 3 h), LPS treated (100 ng ml−1, 3 h) & LPS+ERG240 treated (LPS, 100 ng ml−1; ERG240, 20 mM for 3 h) hMDMs. The experiment is representative of three independent experiments using n=3 healthy donor hMDMs each. (f) GC/MS results showing itaconic acid production in hMDMs in presence or absence of ERG240 (20 mM). LPS treatment was for 8 h (100 ng ml−1). GC/MS plot for each donor is shown separately where itaconate abundance denotes arbitrary units. Error bars are s.e.m. Significance was tested using two-tailed Student’s t-test or one-way ANOVA. * P" data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody, Catalog # NB100-449" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-449_rabbit-polyclonal-hif-1-alpha-antibody-31020241554544.jpg" data-alt="HIF-1 alpha Antibody" title="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody [NB100-449] -" data-caption="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody [NB100-449] - HIF-1 alpha/2 alpha expression in myeloid-specific KO mice targeting the HIF pathway. (A) Images of the colon of wild-type (WT), myeloid-specific Hif-1a KO (hMRP8 Hif-1a KO) or von Hippel Lindau (Vhl) KO (hMRP8 Vhl KO) mice, immunostained for MRP8 (green) & the DNA-binding regions of Hif-1a mRNA (red). Mice were fed with 5% DSS for 4 days prior to immunostaining analyses. Note that there were no MRP8-positive cells that were positive for Hif-1a mRNA in hMRP8 Hif-1a KO (middle column) mice, but we observed many cells that were double positive for MRP8 & Hif-1a mRNA in hMRP8 Vhl KO mice (right column). (B) Images of the colon of hMRP8 Vhl KO mice fed with 5% DSS as in A, immunostained for MRP8 (green) & HIF-1 alpha (red, upper row) or HIF-2 alpha (red, bottom row). DAPI-stained nuclei are shown in blue. White boxes in A & B indicate the regions magnified in the lower or right images, respectively. Yellow arrowheads in A & B indicate cells positive for both markers. Scale bars: 100 μm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29967068), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Immunocytochemistry/ Immunofluorescence" data-header="HIF-1 alpha Antibody, Catalog # NB100-449" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-449_rabbit-polyclonal-hif-1-alpha-antibody-31020241612613.jpg" data-alt="HIF-1 alpha Antibody" title="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody [NB100-449] -" data-caption="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody [NB100-449] - Increased infiltration of myeloid cells expressing HIF-1 alpha in DSS-induced colitis. (A) Western blot analysis of MRP8, F4/80 or actin using the whole colon tissue lysate from mice fed with water or 5% DSS for 4 days. (B) Images of the colon of mice fed with water or 5% DSS, immunostained for myeloid cells using anti-MRP8 (green) or anti-HIF-1 alpha (red) antibodies. Nuclei were counterstained with DAPI. White rectangles indicate the areas magnified in the images shown below. Yellow arrowheads indicate cells with colocalization of MRP8 & HIF-1 alpha. Quantification of MRP8-positive cells & percentage of those expressing HIF-1 alpha are shown as bar graphs on the right-hand side. Data are mean±s.e.m. for at least three independent fields examined per mouse (n≥3 per group). **P" data-badges="" data-application="Immunocytochemistry/ Immunofluorescence" data-header="HIF-1 alpha Antibody, Catalog # NB100-449" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-449_rabbit-polyclonal-hif-1-alpha-antibody-31020241612635.jpg" data-alt="HIF-1 alpha Antibody" title="Western Blot: HIF-1 alpha Antibody [NB100-449] -" data-caption="Western Blot: HIF-1 alpha Antibody [NB100-449] - Increased HIF-1 alpha expression is regulated by TSLPR inhibition & not IL-1 beta secretion (A) Human mDC were stimulated with SC glucan with either anti-TSLPR or IgG isotype control antibodies for 8 h in the presence or absence of IL-1 beta neutralization antibodies (n = 1 representative donor presented, three separate experiments performed). Pro-IL-1 beta, IL-1 beta, HIF-1 alpha, phospho-p38 MAPK, p38 MAPK, phospho-AMPK, AMPK & beta-actin were measured by immunoblot. (B,C) Densitometry of cumulative data was performed using Image Studio Lite software with HIF-1 alpha normalized to beta-actin & phospho-AMPK normalized to AMPK. Data is reported as percentage of maximal signal observed within each donor (n = 3 independent donors, presented as pooled data). Cumulative data displayed as mean +SEM. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31139177), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody, Catalog # NB100-449" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-449_rabbit-polyclonal-hif-1-alpha-antibody-3102024155453.jpg" data-alt="HIF-1 alpha Antibody" title="Western Blot: HIF-1 alpha Antibody [NB100-449] -" data-caption="Western Blot: HIF-1 alpha Antibody [NB100-449] - miR-18b (miR-18b-5p) regulates Hif1 alpha & reduces apoptosis in mtNSC-34 cells. a Overexpressed miR-18b (miR-18b-5p) decreased Hif1 alpha & Mef2c proteins. Both Mctp1 & Rarb expression were increased by miR-18b (miR-18b-5p). Downregulated Bax & upregulated Bcl2 by miR-18b (miR-18b-5p) diminished apoptosis in mtNSC-34 cells. b RT-qPCR analysis showed low expression of Hif1 alpha & Mef2c mRNAs. c Mctp1 & Rarb transcripts were highly expressed by miR-18b (miR-18b-5p). d Bax mRNAs were decreased & Bcl2 mRNAs were increased by overexpressed miR-18b. e miR-18b (miR-18b-5p) was overexpressed in mtNSC-34 cells. f miR-206 was reduced by miR-18b (miR-18b-5p). g LDH release analysis explained that transfected miR-18b (miR-18b-5p) restores apoptosis. h Luciferases assay with 3′ UTR of Hif1 alpha showed that Hif1 alpha is target of miR-18b in contNSC-34 cells. i & j Overexpression of miR-18b (miR-18b-5p) enhanced neuronal differentiation (MAP2) & attenuated intracellular Ca2+ levels (Cont (0.098) versus miR-18b (miR-18b-5p) (0.051) in fluorescence intensities from baseline 490/525 ratio) in mtNSC-34 cells. Empty vector served as a negative control (Cont). Arrow represents SOD1 aggregation (green). Scale bar, 40 μm. Significantly different at *, p " data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody, Catalog # NB100-449" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-449_rabbit-polyclonal-hif-1-alpha-antibody-31020241554521.jpg" data-alt="HIF-1 alpha Antibody" title="Western Blot: HIF-1 alpha Antibody [NB100-449] -" data-caption="Western Blot: HIF-1 alpha Antibody [NB100-449] - Hypoxic conditions upregulate CNN3 in BeWo cells.BeWo cells were cultured either under normoxic or under hypoxic conditions for 24(A) Total protein lysates were examined with the Western Blot technique to detect CNN3 protein levels. For normalization, HPRT was stained on the same membrane. As hypoxia marker, HIF-1 alpha was detected as well. (B) Protein bands of CNN3 & HPRT were densitometrically measured on the Western Blot membrane & CNN3/HPRT levels are plotted in the graph (white column: normoxia; black column: hypoxia). n = 3. (C) BeWo cells were serum starved for 16 h & then treated with either PBS as control or 200 µM CoCl2 for 6 h in serum free medium. Then total protein was isolated & a Western Blot was performed. The HIF-1 alpha, CNN3 & HPRT protein was detected on the membrane with specific antibodies. (D) A densitometric analysis was performed to determine CNN3/HPRT levels (white column: normoxia; black column: hypoxia). n = 3. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25050546), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody, Catalog # NB100-449" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-449_rabbit-polyclonal-hif-1-alpha-antibody-31020241552522.jpg" data-alt="HIF-1 alpha Antibody" title="Western Blot: HIF-1 alpha Antibody [NB100-449] -" data-caption="Western Blot: HIF-1 alpha Antibody [NB100-449] - Dynamics of the hypoxia-triggered decrease in MET phosphorylation & its reversal upon reoxygenation(A) MCF10A cells were incubated under normoxia or hypoxia for 1 h. They were then treated, under the same oxygen pressure, with 10 ng/mL HGF/SF for 5, 10, 20, 30, 60, 90 & 120 minutes. A control (Ctrl) without any HGF/SF stimulation was also performed. The same amount of protein was analyzed by western blotting with antibodies directed against: phosphorylated residues in the MET kinase domain, the MET kinase domain, the hypoxia marker HIF1a, phosphorylated Akt, Akt, Erk2, phosphorylated Erk, & actine. The positions of prestained molecular weight markers are indicated. Arrows indicate the positions of precursor & mature full-length MET. (B) MCF10A cells were incubated under hypoxia for 5, 10, 15 or 30 minutes. Another set of cells were incubated under hypoxia for 1 h & then returned to normoxia for 5, 10, 15 or 30 min (re-oxygenation). A control under normoxic (N) conditions was also included. The same amount of protein was analyzed by western blotting as previously described with the addition of GAB1 & its phosphorylated form. (C) MCF10A cells were placed under normoxic or hypoxic conditions for 1 h or hypoxia for 1 h & then normoxia for 10 minutes (reoxygenation). Cells were then treated at the indicated time with 10 ng/mL of HGF/SF. Cell lysates were incubated for AlphaScreen specific phospho-Erk & phospho-Akt quantitation. Error bars represent standard deviations (n = 3; ± SD). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29930749), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody, Catalog # NB100-449" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-449_rabbit-polyclonal-hif-1-alpha-antibody-31020241682187.jpg" data-alt="HIF-1 alpha Antibody" title="Western Blot: HIF-1 alpha Antibody [NB100-449] -" data-caption="Western Blot: HIF-1 alpha Antibody [NB100-449] - The role of HIF-1 in purinosome formation.a, quantifying the number of purinosome-containing cells in normoxia or hypoxia (24 h) in purine-rich medium & normoxia in purine-depleted medium (purine -ve), cells in hypoxia transfected with siRNA to HIF-1 alpha (+ siRNA), & cells in purine-rich medium supplemented with DFX. Data shown are n = 3, mean ± S.E., total number of cells counted are shown in parentheses. b, time course of purinosome formation in hypoxia shows the number of purinosome-containing cells steadily increases after 3 h in hypoxia. Re-oxygenation of the samples after hypoxic incubation for 10 h reverts the number purinosome-containing cells back to normoxic levels. Data shown is n = 3, mean ± S.E., total number of cells counted are shown in parentheses. c, time course of HIF-1 alpha stabilization in hypoxia shows maximum HIF-1 alpha protein expression levels at 3 h in hypoxia, after which the HIF-1 alpha expression decreases. The positions of molecular markers are shown for each blot; uncropped blots with overlaid markers are deposited in the raw data files. d, the effect of hypoxia on the transcription of purine biosynthesis enzymes measured by qPCR. Vascular endothelial growth factor (VEGF) & HIF-1 alpha are controls. Data shown are n = 5, mean ± S.E. e, the effect of hypoxia on the protein expression levels of the purine biosynthetic enzymes. HIF-1 alpha is stabilized in hypoxia as expected, & no significant increase in the purine enzymes was detected between normoxic (21% oxygen) & hypoxic (1% oxygen) growth conditions. The positions of molecular markers surrounding each band of interest are shown for each blot; uncropped blots with overlaid markers are deposited in the raw data files. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32439803), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody, Catalog # NB100-449" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-449_rabbit-polyclonal-hif-1-alpha-antibody-31020241683944.jpg" data-alt="HIF-1 alpha Antibody" title="Western Blot: HIF-1 alpha Antibody [NB100-449] -" data-caption="Western Blot: HIF-1 alpha Antibody [NB100-449] - C/EBP delta promotes HIF-1 alpha expression in macrophages through inhibition of FBXW7 alphaa) RT-PCR analysis of FBXW7 isoform expression from different sources as follows. 1, primary peritoneal macrophages (PPMs); 2, RAW 264.7 cells; 3, MMTV-Neu mammary tumour tissue; 4, primary mouse embryo fibroblasts. Numbers indicate the position of size markers in base pairs. (b) RT-qPCR analysis of Fbxw7 transcript levels in PPMs from WT & Cebpd−/− KO mice, cultured +/− LPS (100 ng/ml, 24 h), compared to WT untreated (n=4, *P" data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody, Catalog # NB100-449" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-449_rabbit-polyclonal-hif-1-alpha-antibody-31020241612654.jpg" data-alt="HIF-1 alpha Antibody" title="Western Blot: HIF-1 alpha Antibody [NB100-449] -" data-caption="Western Blot: HIF-1 alpha Antibody [NB100-449] - Hypoxia downregulates CFTR expression. (a) Phase-contrast photographs of MDCK cells treated with normoxia & hypoxia conditions; (b) Western blot showing downregulation of CFTR in the MDCK cells by hypoxia (Full-length blot is shown in Supplementary Figure S7b.); (c) Real time-PCR assay showing decreased mRNA expression of CFTR induced by hypoxia in HK-2 cells,*p " data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody, Catalog # NB100-449" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-449_rabbit-polyclonal-hif-1-alpha-antibody-310202415533837.jpg" data-alt="HIF-1 alpha Antibody" title="Western Blot: HIF-1 alpha Antibody [NB100-449] -" data-caption="Western Blot: HIF-1 alpha Antibody [NB100-449] - NRP1 & MET on HT1080 cells are accessible for blood-borne rhodocetin-alpha beta(A) expression of NRP1 & MET by HT1080 cells was proven by flow cytometry. Gray, isotype-matched controls. (B) flow cytometry of NRP1-knockout HT1080 cells demonstrating their NRP1-deficiency & unaffected MET expression. (C) treatment with CoCl2, mimicking a hypoxic tumor micro-environment, induced upregulation of HIF-1 alpha. (D) NRP1, as a downstream target of HIF-1 alpha, is upregulated in HT1080 cells but not in NRP1-knockout HT1080 cells. beta-actin immunoblots show even loading. (E) increased HIF-1 alpha (red) levels in hypoxic tumor regions, which also contained partly (arrows) or completely (open arrows) EC-deficient VM vessels. ECs are stained green & nuclei blue. (F) immunostaining of NRP1 (red) & MET (blue) showed that both proteins were present on HT1080 cells & ECs in tumor tissue. Note the continuity between EC-lined vasculature & EC marker-deficient vessels (arrows in F’). F’, F’’, in oblique view, gating of the green CD31 signal also showed an apical absence of NRP1 on ECs in contrast to MET (open arrows). (G) the fluorescence intensity along a traceroute, averaged over a width of 5 pixels, (rectangle in F) through the endothelium revealed that in ECs NRP1, unlike MET, is absent from the apical side & restricted to the basolateral side. In contrast, on ATV/VM-lining cells (F’, arrows) both NRP1 & MET are accessible from the bloodstream (H). Vertical gray lines in G & H indicate the position of the apical cell border. Original magnification was 400× (E) & 630× (F-F’’). Representative images are shown. Image collected & cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.25032), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody, Catalog # NB100-449" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-449_rabbit-polyclonal-hif-1-alpha-antibody-31020241612628.jpg" data-alt="HIF-1 alpha Antibody" title="Immunohistochemistry: HIF-1 alpha Antibody [NB100-449] -" data-caption="Immunohistochemistry: HIF-1 alpha Antibody [NB100-449] - NRP1 & MET on HT1080 cells are accessible for blood-borne rhodocetin-alpha beta(A) expression of NRP1 & MET by HT1080 cells was proven by flow cytometry. Gray, isotype-matched controls. (B) flow cytometry of NRP1-knockout HT1080 cells demonstrating their NRP1-deficiency & unaffected MET expression. (C) treatment with CoCl2, mimicking a hypoxic tumor micro-environment, induced upregulation of HIF-1 alpha. (D) NRP1, as a downstream target of HIF-1 alpha, is upregulated in HT1080 cells but not in NRP1-knockout HT1080 cells. beta-actin immunoblots show even loading. (E) increased HIF-1 alpha (red) levels in hypoxic tumor regions, which also contained partly (arrows) or completely (open arrows) EC-deficient VM vessels. ECs are stained green & nuclei blue. (F) immunostaining of NRP1 (red) & MET (blue) showed that both proteins were present on HT1080 cells & ECs in tumor tissue. Note the continuity between EC-lined vasculature & EC marker-deficient vessels (arrows in F’). F’, F’’, in oblique view, gating of the green CD31 signal also showed an apical absence of NRP1 on ECs in contrast to MET (open arrows). (G) the fluorescence intensity along a traceroute, averaged over a width of 5 pixels, (rectangle in F) through the endothelium revealed that in ECs NRP1, unlike MET, is absent from the apical side & restricted to the basolateral side. In contrast, on ATV/VM-lining cells (F’, arrows) both NRP1 & MET are accessible from the bloodstream (H). Vertical gray lines in G & H indicate the position of the apical cell border. Original magnification was 400× (E) & 630× (F-F’’). Representative images are shown. Image collected & cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.25032), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Immunohistochemistry" data-header="HIF-1 alpha Antibody, Catalog # NB100-449" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-449_rabbit-polyclonal-hif-1-alpha-antibody-310202415533827.jpg" data-alt="HIF-1 alpha Antibody" title="Western Blot: HIF-1 alpha Antibody [NB100-449] -" data-caption="Western Blot: HIF-1 alpha Antibody [NB100-449] - C/EBP delta promotes HIF-1 alpha expression in macrophages through inhibition of FBXW7 alphaa) RT-PCR analysis of FBXW7 isoform expression from different sources as follows. 1, primary peritoneal macrophages (PPMs); 2, RAW 264.7 cells; 3, MMTV-Neu mammary tumour tissue; 4, primary mouse embryo fibroblasts. Numbers indicate the position of size markers in base pairs. (b) RT-qPCR analysis of Fbxw7 transcript levels in PPMs from WT & Cebpd−/− KO mice, cultured +/− LPS (100 ng/ml, 24 h), compared to WT untreated (n=4, *P" data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody, Catalog # NB100-449" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-449_rabbit-polyclonal-hif-1-alpha-antibody-31020241682146.jpg" data-alt="HIF-1 alpha Antibody" title="Western Blot: HIF-1 alpha Antibody [NB100-449] -" data-caption="Western Blot: HIF-1 alpha Antibody [NB100-449] - Dectin-1-induced TSLP negatively regulates pro-IL-1 beta & HIF-1 alpha. (A) Human mDC were stimulated with SC glucan, CA glucan or heat killed C. albicans hyphae with anti-TSLPR antibodies or IgG isotype control for 24 h (n = 6 independent donors, presented as pooled data). Lactate production was measured in cell-culture supernatants using colourmetric L-lactate detection kit. (B) Human mDC were stimulated SC glucan with either anti-TSLP, anti-TSLPR or IgG isotype control antibodies for 8 h (n = 1 representative donor presented, three separate experiments performed). Pro-IL-1 beta, IL-1 beta, HIF-1 alpha, phospho-p38 MAPK, p38 MAPK, phospho-AMPK, AMPK & beta-actin were measured by immunoblot. (C–G) Densitometry of cumulative data was performed using Image Studio Lite software with pro-IL-1 beta, IL-1 beta & HIF-1 alpha normalized to beta-actin & phospho-p38 MAPK & phospho-AMPK normalized to total p38 MAPK & AMPK respectively. Data is reported as percentage of maximal signal observed within each donor (n = 3 independent donors, presented as pooled data). Cumulative data displayed as mean +SEM. Statistical analysis calculated using one-way ANOVA with Bonferroni post-tests (***p = 0.001). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31139177), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody, Catalog # NB100-449" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-449_rabbit-polyclonal-hif-1-alpha-antibody-31020241682155.jpg" data-alt="HIF-1 alpha Antibody" title="Western Blot: HIF-1 alpha Antibody [NB100-449] -" data-caption="Western Blot: HIF-1 alpha Antibody [NB100-449] - Involvement of MET in Akt & Erk pathway activation under hypoxiaMCF10A cells were transfected with a pool of three MET-targeting siRNAs (20 nM) or a control siRNA (siCtrl). A control without siRNA was also included (Ctrl) (A). MCF10A cells were transfected with two HIF1a-targeting siRNAs (20 nM), independently or together, or a control siRNA (siCtrl) (B). The cells were then placed for 1 h under normoxic or hypoxic conditions & treated or not for 10 min with 10 ng/mL HGF/SF. In each experiment, the same amount of protein was analyzed by western blotting with antibodies directed against: phosphorylated residues in the MET kinase domain, the MET kinase domain, phosphorylated Akt, Akt, phosphorylated Erk, Erk2, phosphorylated GAB1, GAB1, or hypoxia marker HIF1a. The positions of prestained molecular weight markers are indicated. Arrows indicate the positions of precursor & mature full-length MET & Erk1/2 proteins. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29930749), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody, Catalog # NB100-449" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-449_rabbit-polyclonal-hif-1-alpha-antibody-310202415541992.jpg" data-alt="HIF-1 alpha Antibody" title="Western Blot: HIF-1 alpha Antibody [NB100-449] -" data-caption="Western Blot: HIF-1 alpha Antibody [NB100-449] - beta-Glucan size affects the requirement for reactive oxygen species (ROS) in IL-1 beta induction. (A) Human monocyte-derived dendritic cell (mDC) stimulated with curdlan or glucan-mp for 8 h. ROS were detected by incubating cells with CellRox Green fluorescence dye & analysis by flow cytometry (representative experiment of two) presented, (B) Human mDCs from healthy donors or chronic granulomatous disease (CGD) patients were stimulated with curdlan or glucan-mp for 8 h. HIF-1 alpha & pro-IL-1 beta protein expression measured by immunoblot (n = 2 donors). (C–L) Human mDC from healthy donors or CGD patients were stimulated with curdlan or glucan-mp for 24 h (n = 6 donors). IL-1 beta, IL-6, IL-23, TSLP, & CCL22 secretion were measured by enzyme-linked immunosorbent assay, with cumulative data displayed as mean ± SEM. Image collected & cropped by CiteAb from the following publication (http://journal.frontiersin.org/article/10.3389/fimmu.2017.00791/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody, Catalog # NB100-449" data-width="250" data-height="250" class="none"></i> </div> </div> </div> </div> <div class="atc-block-search atc-block-collapsible" data-type="search" data-blockid="ProductCart-NB100-449" > <form action="/commerce/addtocart" method="post" class="px-3 pt-3 add-to-cart-block" data-productcode="NB100-449"> <div class="commerce-atc-full-header atc-header col-four row border-bottom pb-1 m-0"> <div class="commerce-atc-catnum atc-head col-4 col-md-4 font-weight-bold">Catalog #</div> <div class="commerce-atc-availability atc-head col-md-3 font-weight-bold d-none d-md-block">Availability </div> <div class="commerce-atc-price atc-head col-5 col-md-3 font-weight-bold">Size / Price</div> <div class="commerce-atc-quantity atc-head col-3 col-md-2 text-right font-weight-bold">Qty</div> </div> <div class="spinner-border spinner-border-sm mt-3 ml-3" role="status"> <span class="sr-only">Loading...</span> </div> <div class="d-none search-product-mask"> <div id="ProductCart-NB100-449" class="atc-lines"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="NB100-449"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> NB100-449 </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="NB100-449SS"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> NB100-449SS </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> </div> <div class="atc-control ProductCart-NB100-449-control text-right"> <div class="row d-flex justify-content-end mx-0 p-0"> <div class="order-md-last m___button-atc mx-1"> <button type="submit" value="Add To Cart" class="atc-add-button btn a___button-atc m-1 col-xs-4 order-last btn-md-block w-100"> Add To Cart </button> </div> <div class="m___button-atc mx-1"> <a href="/services/bulk-quotes?product_code=NB100-449" class="atc-bulk-button btn a___button-secondary m-1 col-xs-4 btn-md-block w-100" rel="nofollow">Bulk Order</a> </div> </div> </div> </div> </form> </div> </div> </div> </div> <div class="search_result atc-container pb-3" data-brand="novus" data-category="Primary Antibodies" data-id="NB100-134" data-name="HIF-1 alpha Antibody - BSA Free" data-position="3"> <div class="search_details border bg-white p-3 rounded"> <div class="row"> <div class="col-md-9"> <a href="/p/antibodies/hif-1-alpha-antibody_nb100-134" title="HIF-1 alpha Antibody - BSA Free"> <h4>HIF-1 alpha Antibody - BSA Free </h4> </a> </div> <div class="search_result_brand_logo col-md-3 mb-1 d-flex justify-content-xl-end product-brand-logo"> <img alt="Novus Biologicals, a Bio-Techne brand" src="https://resources.bio-techne.com/bio-techne-assets/images/logos/bio-techne-novus-biologicals.svg" class="d-flex w-75"> </div> </div> <div class="row"> <div class="col-md-9"> <div class="mb-1 d-inline-flex"> <div class="align-middle my-1 mr-3 d-block d-md-inline"> Catalog #: <a href="/p/antibodies/hif-1-alpha-antibody_nb100-134" title="HIF-1 alpha Antibody - BSA Free"> NB100-134 </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold"> <a href="/p/antibodies/hif-1-alpha-antibody_nb100-134#reviews" class="searchImage_star5"> (14) </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold"> <a href="/p/antibodies/hif-1-alpha-antibody_nb100-134#citations"><span class="icon-icon-product-citations"></span>Citations (236)</a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold text-nowrap"> <a href="/p/antibodies/hif-1-alpha-antibody_nb100-134#product-documents"><span class="icon-icon-pdf text-error-red"></span> Product Documents</a> </div> <div class="d-none d-md-inline my-1"> <a href="#" class="search_result_images"><span class="icon-icon-image-camera-plus"></span> Images (39)</a> </div> </div> <div class="d-none d-md-block"> <span class="search_result_applications"></span><span class="applications_title"><strong>Applications:</strong></span> IHC, WB, ELISA, ICC/IF, IP, B/N, ChIP, Dual ISH-IHC, Func, GS, KD, KO, Simple Western </div> <div class="d-none d-md-block"> <span class="species_title"><strong>Reactivity:</strong></span> Human, Mouse, Rat, Canine, Bovine, +7 More </div> </div> <div class="col-md-3"> <button class="py-1 btn-default btn-block atc-view-more collapsed" data-toggle="collapse" href="#collapseButton-3" role="button" aria-expanded="false" aria-controls="collapseButton">View More</button> </div> </div> <div class="commerce-atc-search pt-3 collapse" id="collapseButton-3"> <div class="row"> <div class="search_result_description col-xs-12 col-md-9"> <table class="tablesaw tablesaw-stack mb-2"> <tbody> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Reactivity:</strong></td> <td class="pb-1 w-75 text-break"> Human, Mouse, Rat, Canine, Bovine, +7 More </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Details:</strong></td> <td class="pb-1 w-75 text-break"> Rabbit IgG Polyclonal </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Applications:</strong></td> <td class="pb-1 w-75 text-break"> IHC, WB, ELISA, ICC/IF, IP, +8 More </td> </tr> </tbody> </table> <div class="compare_tool_select form-inline mb-2" data-sku="nb100-134"></div> </div> <div class="search_result_image_wrapper col-xs-12 col-md-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="novus"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src=https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody---BSA-Free-Western-Blot-NB100-134-img0013.jpg data-srcset=https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody---BSA-Free-Western-Blot-NB100-134-img0013.jpg alt="Western Blot: HIF-1 alpha AntibodyBSA Free [NB100-134]" title="Western Blot: HIF-1 alpha AntibodyBSA Free [NB100-134]" data-caption="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-134] - Analysis of HIF-1 alpha in human retinal and choroidal primary endothelia cells exposed to hypoxic conditions in the times noted using NB100-134. Image from verified customer review." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-134" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> <span class="image-count-text align-middle">(39)</span> </div> </div> </div> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB100-134-img0017.jpg" data-alt="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody - BSA Free [NB100-134]" title="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody - BSA Free [NB100-134]" data-caption="Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody - BSA Free [NB100-134] - Hif1alpha expression in neonatal and adult ovary. Section of P5 ovary showing MVH expression in all oocytes and Hif1alpha expression only in small oocytes (primary follicles; top). Negative control images without primary antibodies (bottom). Scale bar: 50um. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0154309) licensed under a CC-BY license." data-badges="" data-application="Immunocytochemistry/ Immunofluorescence" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-134" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NB100-134-img0014.jpg" data-alt="Immunohistochemistry-Paraffin: HIF-1 alpha Antibody - BSA Free [NB100-134]" title="Immunohistochemistry-Paraffin: HIF-1 alpha Antibody - BSA Free [NB100-134]" data-caption="Immunohistochemistry-Paraffin: HIF-1 alpha Antibody - BSA Free [NB100-134] - Analysis of HIF-1 alpha in zebrafish retina tissue using NB100-134 HIF-1 alpha antibody. Mouse tissue was used as a control. Image from verified customer review." data-badges="" data-application="Immunohistochemistry-Paraffin" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-134" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody---BSA-Free-Western-Blot-NB100-134-img0011.jpg" data-alt="Western Blot: HIF-1 alpha AntibodyBSA Free [NB100-134]" title="Western Blot: HIF-1 alpha AntibodyBSA Free [NB100-134]" data-caption="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-134] - Analysis of HIF-1 alpha in overexpression and endogenous HIF-1 alpha & HIF-2 alpha using anti-HIF-1 alpha antibody NB100-134. The data showed that HIF-1 alpha antibody did not react to HIF-2 alpha overexpression. Image from verified customer review." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-134" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody---BSA-Free-Western-Blot-NB100-134-img0007.jpg" data-alt="Western Blot: HIF-1 alpha AntibodyBSA Free [NB100-134]" title="Western Blot: HIF-1 alpha AntibodyBSA Free [NB100-134]" data-caption="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-134] - Analysis of HIF-1 alpha using NB100-134, on normoxic and hypoxic nuclear rat cell lysates." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-134" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1 alpha Antibody - BSA Free-Immunocytochemistry-Immunofluorescence-NB100-134-img0020.jpg" data-alt="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody - BSA Free [NB100-134]" title="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody - BSA Free [NB100-134]" data-caption="Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody - BSA Free [NB100-134] - Representative images of macrophages and activated macrophages, in the absence or presence of 10 uM hemin for 72 h. HIF-1 alpha levels were detected by immunofluorescence using a confocal imaging system (Opera Phenix, Perkin Elmer). Images were acquired with 20x water objective. Staining intensity levels in the nucleus and cytosolic region were obtained using Harmony software (Perkin Elmer). Nucleus and cytosol were identified through Hoechst (at 405 nm) and CellMask (at 655 nm) staining, respectively. Scale is shown as 100 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33187129/) licensed under a CC-BY license." data-badges="<span class="biological-validation"></span>" data-application="Immunocytochemistry/ Immunofluorescence" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-134" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB100-134-img0010.jpg" data-alt="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody - BSA Free [NB100-134]" title="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody - BSA Free [NB100-134]" data-caption="Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody - BSA Free [NB100-134] - Analysis of HIF-1 alpha in Normoxic (N) and Hypoxic (H) ARPE-19 cells using anti-HIF-1 alpha antibody NB100-134. Image from verified customer review." data-badges="" data-application="Immunocytochemistry/ Immunofluorescence" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-134" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB100-134-img0016.jpg" data-alt="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody - BSA Free [NB100-134]" title="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody - BSA Free [NB100-134]" data-caption="Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody - BSA Free [NB100-134] - Hif1alpha expression in 9.5dpc primordial germ cells. Whole-mount staining of Hif1alpha and GFP in Oct4-GFP embryo (lateral view). Scale bar: 100um. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0154309) licensed under a CC-BY license." data-badges="" data-application="Immunocytochemistry/ Immunofluorescence" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-134" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NB100-134-img0008.jpg" data-alt="Immunohistochemistry-Paraffin: HIF-1 alpha Antibody - BSA Free [NB100-134]" title="Immunohistochemistry-Paraffin: HIF-1 alpha Antibody - BSA Free [NB100-134]" data-caption="Immunohistochemistry-Paraffin: HIF-1 alpha Antibody - BSA Free [NB100-134] - Staining in the canine CL on days 10 to 70 after ovulation. PC = positive control (human placenta). NC = negative control. Image from verified customer review." data-badges="" data-application="Immunohistochemistry-Paraffin" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-134" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody---BSA-Free-Immunohistochemistry-NB100-134-img0006.jpg" data-alt="Immunohistochemistry: HIF-1 alpha Antibody - BSA Free [NB100-134]" title="Immunohistochemistry: HIF-1 alpha Antibody - BSA Free [NB100-134]" data-caption="Immunohistochemistry: HIF-1 alpha Antibody - BSA Free [NB100-134] - Staining of human kidney, renal tubular epithelium in cortex using NB100-134." data-badges="" data-application="Immunohistochemistry" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-134" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NB100-134-img0012.jpg" data-alt="Immunohistochemistry-Paraffin: HIF-1 alpha Antibody - BSA Free [NB100-134]" title="Immunohistochemistry-Paraffin: HIF-1 alpha Antibody - BSA Free [NB100-134]" data-caption="Immunohistochemistry-Paraffin: HIF-1 alpha Antibody - BSA Free [NB100-134] - Analysis of FFPE tissue section of human endometrium carcinoma AN3CA cell line based xenograft using rabbit polyclonal HIF-1 alpha antibody NB100-134 at 1:300 dilution. The signal was developed using HRP-labelled secondary antibody and DAB reagent, and the section was further counterstained using hematoxylin. The tested section depicted mainly a diffused cytoplasmic staining but there were some cells which showed nuclear signal also (representing hypoxic cells)." data-badges="" data-application="Immunohistochemistry-Paraffin" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-134" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody---BSA-Free-Simple-Western-NB100-134-img0009.jpg" data-alt="Simple Western: HIF-1 alpha AntibodyBSA Free [NB100-134]" title="Simple Western: HIF-1 alpha AntibodyBSA Free [NB100-134]" data-caption="Simple Western: HIF-1 alpha Antibody - BSA Free [NB100-134] - Image shows a specific band for HIF-1 alpha in 0.2 mg/mL of Hypoxic HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system." data-badges="" data-application="Simple Western" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-134" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody---BSA-Free-Immunoprecipitation-NB100-134-img0001.jpg" data-alt="Immunoprecipitation: HIF-1 alpha Antibody - BSA Free [NB100-134]" title="Immunoprecipitation: HIF-1 alpha Antibody - BSA Free [NB100-134]" data-caption="Immunoprecipitation: HIF-1 alpha Antibody - BSA Free [NB100-134] - PC-3 cell lysates run with cobalt chloride treatments. Immunoprecipitation was performed with protein A/G agarose beads. This data was provided courtesy of Kelie Reece, Figg lab, NCI." data-badges="<span class="biological-validation"></span>" data-application="Immunoprecipitation" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-134" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody---BSA-Free-Dual-RNAscope-ISH-IHC-NB100-134-img0018.jpg" data-alt="" title="" data-caption="Dual RNAscope ISH-IHC: HIF-1 alpha Antibody - BSA Free [NB100-134] - Formalin-fixed paraffin-embedded tissue sections of human kidney were probed for HIF-1 alpha mRNA (ACD RNAScope Probe, catalog #605228; Fast Red chromogen, ACD catalog # 322750). Adjacent tissue section was processed for immunohistochemistry using Rabbit Polyclonal (Novus Biologicals catalog # NB100-134) at 1:500 dilution with one-hour incubation at room temperature followed by incubation with anti-rabbit IgG VisUCyte HRP Polymer Antibody (Catalog # VC003) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of tubules." data-badges="" data-application="Dual RNAscope ISH-IHC" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-134" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody---BSA-Free-Simple-Western-NB100-134-img0019.jpg" data-alt="Simple Western: HIF-1 alpha AntibodyBSA Free [NB100-134]" title="Simple Western: HIF-1 alpha AntibodyBSA Free [NB100-134]" data-caption="Simple Western: HIF-1 alpha Antibody - BSA Free [NB100-134] - Simple Western lane view shows lysates of MCF +/- DMOG, BioSpherix MSCs in hypoxic conditions, and BioSpherix MSCs in normoxic conditions loaded at 0.5 mg/ml. A band was detected for HIF-1 alpha at approximately 116 kDa (as indicated) using NB100-134 (1:100 dilution) followed by Anti-Rabbit Secondary Antibody (042-206, ProteinSimple). This experiment was conducted under standard assay conditions, and using the 12-230 kDa separation module (SW-W004). Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody. Image from an internal validation." data-badges="" data-application="Simple Western" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-134" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-134_rabbit-polyclonal-hif-1-alpha-antibody-271220231253384.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-134] -" data-caption="P53 was increased response to IR in the fetal brain.Even though no significant change in protein level, total p53 and phosphorylation of P53 at ser15 were increased compared with HIF-1 alpha in fetal brains response to IR. Numbers in the pictures show the average of three independent experiments (n = 3 from 3 individual pregnant mice)." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-134" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-134_rabbit-polyclonal-hif-1-alpha-antibody-210202423454829.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-134] -" data-caption="WNT11 is induced by hypoxia or hypoxic mimetics in different cell types.(A) Increased Wnt11 mRNA in EMSC adipocytes (Day 12) after hypoxia-mimetic treatments. EMSC adipocytes were treated with CoCl2 (0.1 mM), DFO (0.1 mM) or DMOG (0.1 mM) for 24 hrs. Values were normalized to Tbp mRNA & are expressed relative to control (n = 3). (B,C) Increased Wnt11 mRNA by hypoxia in EMSC preadipocytes & adipocytes (Day 0–12 after differentiation) (B), & C2C12 myoblast & myocyte (Day 0 & 8 after differentiation) (C). Wnt11 mRNA was assessed by quantitative PCR in cells exposed to air (21% O2) or hypoxia (1% O2) for 24 hrs. (n = 4). Values were normalized to Tbp mRNA & are expressed relative to 21% O2 samples (left panel). (D) Immunoblot analyses of HeLa cells under normal air or hypoxia for 24 hrs. (E,F) Induction of Wnt11 by increasing concentrations of DMOG in MDA-MB-231 cells (E) & 4T1 cells (F). (G) EMSCs treated with 0.1 mM DMOG for the indicated times. Wnt11 & Vegf mRNA expression was measured by qPCR & normalized to Tbp mRNA (n = 4). (H) WNT11 protein levels after DMOG treatment normalized to alpha-Tubulin (upper panel; n = 4). Representative immunoblots of EMSCs treated with 0.1 mM DMOG for the indicated times (Lower panel). (I) Protein expression in MDA-MB-231 cells treated with 0.1 mM DMOG. (J) Induction of Wnt11 promoter activity by hypoxia or hypoxia mimetics. pGL3-Wnt11 promoter plasmid was transfected into C2C12 cells. Cells were incubated with DMOG (left panel, n = 4) or under 21% O2 or 1% O2 (right panel, n = 8) for 24 hrs. For panels (A–C,G,H,J), values are mean ± s.e.m. *p " data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-134" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-134_rabbit-polyclonal-hif-1-alpha-antibody-310202415394545.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-134] -" data-caption="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-134] - LOXL2 abundance in EC‐derived exosomes is increased in hypoxia. (A) Immunoblots of endothelial cells for HIF‐1 alpha (control for hypoxia, top panel), LOXL2 (middle panel) & beta‐actin (loading control, lower panel). (B) Immunoblots of EC‐derived exosomes for LOXL2 (upper panel), & beta‐actin (loading control, lower panel). (C) Densitometric quantification of relative LOXL2 protein abundance in control & hypoxic EC‐derived exosomes (n = 4 ± SD, Student's t‐test; **P < 0.01). (D) Immunoblots of sucrose density gradient samples of EC‐derived exosomes for LOXL2 (upper panel) & exosome‐marker Flotillin‐1 (lower panel). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26612622), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-134" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-134_rabbit-polyclonal-hif-1-alpha-antibody-310202415384157.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody - BSA Free [NB100-134] -" data-caption="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody - BSA Free [NB100-134] - Hif1 alpha expression in 9.5dpc primordial germ cells.(A) Whole-mount staining of Hif1 alpha & GFP in Oct4-GFP embryo (lateral view). Scale bar: 100μm. (B) Hif1 alpha & GFP staining of FACS-sorted PGCs. Scale bar: 20μm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27148974), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Immunocytochemistry/ Immunofluorescence" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-134" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-134_rabbit-polyclonal-hif-1-alpha-antibody-310202415363748.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-134] -" data-caption="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-134] - Hypoxic setup for larval zebrafish. (A) (1) oxygen regulator; (2) hypoxic larval aquarium; (3) oxygen electrode; (4) air stone; & (5) solenoid valve. The compressed nitrogen source is shown as a blue gas cylinder. (B) Dissolved oxygen in the hypoxic aquarium decreased slowly over time as nitrogen perfusion increased until dissolved oxygen stabilized at 3.5 mg/L. (C) qRT-PCR analysis showing that hif1 alpha mRNA expression was significantly increased under hypoxia. (D) Western blot of Hif1 alpha protein expression showing that hypoxia enhanced Mbp protein translation. ∗P < 0.05; ∗∗P < 0.01; ∗∗∗P < 0.001. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30337858), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-134" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-134_rabbit-polyclonal-hif-1-alpha-antibody-310202415384174.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-134] -" data-caption="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-134] - P53 was increased response to IR in the fetal brain.Even though no significant change in protein level, total p53 & phosphorylation of P53 at ser15 were increased compared with HIF-1 alpha in fetal brains response to IR. Numbers in the pictures show the average of three independent experiments (n = 3 from 3 individual pregnant mice). Image collected & cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0110577), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-134" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-134_rabbit-polyclonal-hif-1-alpha-antibody-31020241535619.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody - BSA Free [NB100-134] -" data-caption="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody - BSA Free [NB100-134] - Hif1 alpha expression in 15.5dpc germ cells.(A) Sections of male (top) & female (bottom) gonads from 15.5 dpc Oct4-GFP embryos showing Oct4-GFP & Hif1 alpha expressions in germ cells. Scale bars: 50μm (male) & 20μm (female). (B) FACS-sorted male (top) & female (bottom) 15.5dpc germ cells showing Oct4-GFP & Hif1 alpha expressions. Scale bars: 100μm (male) & 50μm (female). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27148974), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Immunocytochemistry/ Immunofluorescence" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-134" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-134_rabbit-polyclonal-hif-1-alpha-antibody-310202415382410.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody - BSA Free [NB100-134] -" data-caption="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody - BSA Free [NB100-134] - Hif1 alpha expression in preimplantation embryos.(A) Hif1 alpha staining in two-cell stage embryo & morula (top) & negative control images without primary antibody (bottom). (B) Co-staining of OCT4 & Hif1 alpha in inner cell mass of blastocyst (top) & negative control images without primary antibodies (bottom). Scale bar: 30μm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27148974), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Immunocytochemistry/ Immunofluorescence" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-134" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-134_rabbit-polyclonal-hif-1-alpha-antibody-310202415523948.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-134] -" data-caption="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-134] - HIF-1 alpha binds to TIM-3 promoter & regulates its expression in primary glia.(a) Cell surface expression of TIM-3 analysed in BV2 cells under 20% O2 or 1% O2 for 24 h by flow cytometry using PE-conjugated anti-TIM-3 antibody. Results from 3 independent experiments presented as a representative histogram & the mean fold change (± s.d.) relative to normoxic sample. (b) Mouse primary mixed glial cells incubated under hypoxia or normoxia for 24 h, & the cells examined by immunocytochemistry using an anti-TIM-3 antibody. (c,d) Mouse primary mixed glial cells & primary neuronal cells incubated under hypoxia or normoxia for 24 h, & then RT–PCR used to detect the levels of TIM-3 & actin. Relative transcript levels shown as the mean fold change (± s.d.) from 3 independent experiments (NS, not significant, Student–Newman–Keuls test). (e) Primary mixed glial cells incubated under hypoxia or normoxia for 24 h, & chromatin immunoprecipitation (ChIP) performed w/ anti-HIF-1 alpha or control IgG. Results presented as relative amounts representative of 3 independent experiments. (f) Primary mixed glial cells cultured from HIF-1 alpha+f/+f mice, infected w/ Ad-GFP or Ad-Cre/GFP, transfected w/ TIM-3-luciferase reporter constructs & incubated under hypoxic or normoxic conditions for 24 h. Relative promoter activity is expressed as the ratio of luciferase activity/ beta-galactosidase activity. (g,h) RT–PCR (g) & WB analysis (h) performed under hypoxia or normoxia for 24 h using the indicated primers & antibodies, respectively. The data shown representative of at least 3 independent experiments. The graphs show the % changes in TIM-3 transcript & protein levels in Ad-Cre/GFP- versus Ad-GFP-infected cells under hypoxia. IP, immunoprecipitation. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25790768), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-134" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-134_rabbit-polyclonal-hif-1-alpha-antibody-31020241555217.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-134] -" data-caption="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-134] - TIM-3 is highly expressed in hypoxic brain regions of a H/I mouse model.(a) TIM-3 transcript levels were examined in brain tissues from the contralateral cortex (C, boxed region) & ischaemic ipsilateral cortex (I, boxed region) of mouse model 24 h after H/I. The RT–PCR products were quantified with Image J & normalized with respect to the expression of actin. The HIF-1 alpha transcript level represents a positive control for hypoxia. The right panel shows representative TTC staining of three brain sections from the H/I mice. (b) Representative western blot analyses of the TIM-3 & HIF-1 alpha proteins (n=3). Relative levels of TIM-3 are shown as the mean±s.d. from three independent experiments. (c) Contralateral & ipsilateral cortical regions of coronal sections from the H/I mice were subjected to immunohistochemistry using an anti-TIM-3 antibody, & the number of TIM-3-expressing cells per mm2 was counted. (d) Immunohistochemistry was performed on brain sections from the H/I mice using anti-TIM-3 & hypoxyprobe-1 (red, to detect hypoxic regions). Scale bars, 50 μm ( × 20); 50 μm ( × 40). (e,f) Brain cells were isolated from the ipsilateral & contralateral hemispheres of three mice per group, processed for simultaneous detection of TIM-3 plus Iba-1 (e) or GFAP (f), & analysed by FACS. The results are presented as relative TIM-3 levels in the indicated gated populations, as determined from three independent experiments. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25790768), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-134" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-134_rabbit-polyclonal-hif-1-alpha-antibody-31020241682166.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-134] -" data-caption="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-134] - Hypoxia & HIF-1 trigger CREB activity.a Knocking down CREB with siRNA abolishes the effect of hypoxia on endogenous rat Gh transcription in GH3 cells as determined by real-time RT-PCR. Immunoblot shows the knockdown efficacy of the CREB siRNA (b) effect of hypoxia (1% O2 for 18 h) on CRE induced luciferase activity. Transfection with 100 nM HIF-1 alpha siRNA for 48 h abolished the effect of hypoxia. Luc/ betaGal: luciferase: beta-galactosidase ratio. Data are means ± SEM of three experiments & expressed as percentage of each normoxia control. *P " data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-134" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-134_rabbit-polyclonal-hif-1-alpha-antibody-310202415532066.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-134] -" data-caption="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-134] - TIM-3 is highly expressed in hypoxic brain regions of a H/I mouse model.(a) TIM-3 transcript levels were examined in brain tissues from the contralateral cortex (C, boxed region) & ischaemic ipsilateral cortex (I, boxed region) of mouse model 24 h after H/I. The RT–PCR products were quantified with Image J & normalized with respect to the expression of actin. The HIF-1 alpha transcript level represents a positive control for hypoxia. The right panel shows representative TTC staining of three brain sections from the H/I mice. (b) Representative western blot analyses of the TIM-3 & HIF-1 alpha proteins (n=3). Relative levels of TIM-3 are shown as the mean±s.d. from three independent experiments. (c) Contralateral & ipsilateral cortical regions of coronal sections from the H/I mice were subjected to immunohistochemistry using an anti-TIM-3 antibody, & the number of TIM-3-expressing cells per mm2 was counted. (d) Immunohistochemistry was performed on brain sections from the H/I mice using anti-TIM-3 & hypoxyprobe-1 (red, to detect hypoxic regions). Scale bars, 50 μm ( × 20); 50 μm ( × 40). (e,f) Brain cells were isolated from the ipsilateral & contralateral hemispheres of three mice per group, processed for simultaneous detection of TIM-3 plus Iba-1 (e) or GFAP (f), & analysed by FACS. The results are presented as relative TIM-3 levels in the indicated gated populations, as determined from three independent experiments. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25790768), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-134" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-134_rabbit-polyclonal-hif-1-alpha-antibody-310202415523935.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody - BSA Free [NB100-134] -" data-caption="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody - BSA Free [NB100-134] - Hif1 alpha expression in neonatal & adult testis.(A) Section of testis from 5-day old (P5) male new born pups showing Hif1 alpha expression in MVH+ gonocytes within the seminiferous tubules (top) & negative control images without primary antibodies (bottom). Scale bar: 50μm. (B) Western blot analysis of Hif1 alpha expression in P5 testes (left) compared to extract of the adult brain sub-ventricular zone (SVZ) (right). Loading control ( beta-Actin) is shown below. (C) Section of adult (3 month old) testis showing Hif1 alpha expression in spermatogonia. Scale bar: 30μm. (D) Western blot analysis of whole adult testis. HEK293 cells treated with DFX were used as a positive control & intestinal tissue was used as a negative control. Loading control ( beta-Actin) is shown below. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27148974), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Immunocytochemistry/ Immunofluorescence" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-134" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-134_rabbit-polyclonal-hif-1-alpha-antibody-31020241552525.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody - BSA Free [NB100-134] -" data-caption="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody - BSA Free [NB100-134] - HIF-1 alpha levels are increased by androgen stimulus & Tip60 overexpression in LNCaP cells. (A) Nuclear & (B) cytosolic HIF-1 alpha levels & (C) images in LNCaP cells & in LNCaP cells overexpressing Tip60, in the absence or presence of androgen (10 nM R1881, 72 h). HIF-1 alpha levels were detected by immunofluorescence using confocal imaging system. Images were acquired with 20x objective. Staining intensity levels in the nucleus & cytosolic region were obtained using Harmony software. Nucleus & cytosol were identified through Hoechst & CellMask staining, respectively. Scale is shown as 100 μm. White dotted frames indicate the section of the image that was enlarged. Values are expressed as mean ± SEM, from three independent culture preparations, each treatment performed in quadruplicate. Two-way ANOVA, Bonferroni post-test & p values comparisons are specified in the figures (* p < 0.05). HIF-1 alpha, hypoxia-inducible factor-1 alpha; OE, overexpressing; R1881, synthetic androgen. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31671779), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Immunocytochemistry/ Immunofluorescence" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-134" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-134_rabbit-polyclonal-hif-1-alpha-antibody-31020241612683.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Chromatin Immunoprecipitation: HIF-1 alpha Antibody - BSA Free [NB100-134] -" data-caption="Chromatin Immunoprecipitation: HIF-1 alpha Antibody - BSA Free [NB100-134] - HIF-1 alpha binds to TIM-3 promoter & regulates its expression in primary glia.(a) Cell surface expression of TIM-3 analysed in BV2 cells under 20% O2 or 1% O2 for 24 h by flow cytometry using PE-conjugated anti-TIM-3 antibody. Results from 3 independent experiments presented as a representative histogram & the mean fold change (± s.d.) relative to normoxic sample. (b) Mouse primary mixed glial cells incubated under hypoxia or normoxia for 24 h, & the cells examined by immunocytochemistry using an anti-TIM-3 antibody. (c,d) Mouse primary mixed glial cells & primary neuronal cells incubated under hypoxia or normoxia for 24 h, & then RT–PCR used to detect the levels of TIM-3 & actin. Relative transcript levels shown as the mean fold change (± s.d.) from 3 independent experiments (NS, not significant, Student–Newman–Keuls test). (e) Primary mixed glial cells incubated under hypoxia or normoxia for 24 h, & chromatin immunoprecipitation (ChIP) performed w/ anti-HIF-1 alpha or control IgG. Results presented as relative amounts representative of 3 independent experiments. (f) Primary mixed glial cells cultured from HIF-1 alpha+f/+f mice, infected w/ Ad-GFP or Ad-Cre/GFP, transfected w/ TIM-3-luciferase reporter constructs & incubated under hypoxic or normoxic conditions for 24 h. Relative promoter activity is expressed as the ratio of luciferase activity/ beta-galactosidase activity. (g,h) RT–PCR (g) & WB analysis (h) performed under hypoxia or normoxia for 24 h using the indicated primers & antibodies, respectively. data shown representative of at least 3 independent experiments. The graphs show the % changes in TIM-3 transcript & protein levels in Ad-Cre/GFP- versus Ad-GFP-infected cells under hypoxia. IP, immunoprecipitation. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25790768), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Chromatin Immunoprecipitation" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-134" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-134_rabbit-polyclonal-hif-1-alpha-antibody-31020241555210.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody - BSA Free [NB100-134] -" data-caption="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody - BSA Free [NB100-134] - NgBR overexpression (OE) suppresses cell proliferation through enhancing mitochondria-ER communication & reducing phosphorylation of IP3R3. (A) The transfection efficiency of NgBR OE plasmid was verified at 48 h post transfection. n = 3 (B) Representative images from three experiments show phosphorylation of endogenous IP3R3 (Akt substrate) in the immunoprecipitates of IP3R3 from crude mitochondrial extracts. (C) Representative confocal microscopy images show co-staining of Rhod-2 AM (red) & MitoTracker Green (green). Results were calculated as relative AFU using ImageJ. n = 20 pictures/group from four independent experiments. Scale bar = 20 µm. (D) Representative line & bar graphs of OCR of control & NgBR OE cells under normoxia. n = 15–16 wells from three individual experiments. (E) Representative confocal microscopy images showing staining of HIF-1 alpha (red) & DAPI (nuclear stain; blue). Percentage of HIF-1 alpha-positive nuclei was calculated using FV10-ASW3.1 software. n > 25 pictures/group from three separate experiments. Scale bar = 40 µm. (F) Cell proliferation was assessed by evaluating PCNA expression, n = 4. * p < 0.05, ** p < 0.01. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31083380), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Immunocytochemistry/ Immunofluorescence" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-134" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-134_rabbit-polyclonal-hif-1-alpha-antibody-310202415535161.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Immunohistochemistry: HIF-1 alpha Antibody - BSA Free [NB100-134] -" data-caption="Immunohistochemistry: HIF-1 alpha Antibody - BSA Free [NB100-134] - E2 enhances neutrophil apoptosis through suppressing the expression of hif1 alpha & c-myb under physiological conditions.A E2 decreased neutrophils in AB zebrafish embryos. (t-test, ***p 20). B May–Grunwald–Giemsa staining of whole KM blood cells in 6-month-old AB zebrafish after 4 days of E2 treatment (t-test, ***p " data-badges="" data-application="Immunohistochemistry" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-134" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-134_rabbit-polyclonal-hif-1-alpha-antibody-31020241682161.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-134] -" data-caption="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-134] - IGFBP5 inhibits HIF1 alpha expression through the MAPK-ERK signaling pathwayA. & B. Western blots using specific antibodies for the phosphorylation state of IGF1R, ERK1/2, & p38-MAPK from A375 IGFBP5 OE & control cells. Phosphorylation of IGF1R, ERK1/2, & p38-MAPK was decreased in A375 cells transfected with IGFBP5. GAPDH was used as a loading control. C. Overexpression of IGFBP5 inhibited HIF1 alpha expression visualized by western blots of A375 cells. The arrow points to the band of HIF1 alpha. D. Assessment of the reduced gene expression levels of VEGFA & MMP9, downstream genes regulated by HIF1 alpha, in A375 IGFBP5 OE compared to control cells by qRT-PCR analysis. Data were shown for the mean ± SD form three independent experiments. P values based on two-side Student t-test comparing A375 IGFBP5 OE tumor cells & vector control cells. *, P < 0.05. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26010068), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-134" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-134_rabbit-polyclonal-hif-1-alpha-antibody-310202415525221.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-134] -" data-caption="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-134] - WNT11 regulates MMPs activities.(A–C) (Top panels): Serum-free medium was conditioned for 24 hrs by the indicated cells, concentrated 20-fold & assayed by gelatin zymography. Gelatinolytic activity is indicated by clear zones against a dark background of stained substrate. (Bottom): Whole cell extracts were immunoblotted with indicated antibodies. (A) Overexpression of Wnt11 in EMSC or BT473 cells enhances activity of MMP-9 & MMP-2. (B) Impaired activity of MMP-9 & MMP-2 in MDA-MB-231 cells (left) or EMSCs (right) stably expressing Wnt11 shRNAs & treated with DMOG. (C) WNT11 is required for MMP-9 & MMP-2 activity in MDA-MB-231 cells (left) or EMSCs (right) under normoxic & hypoxic culture conditions. (D) WNT11 regulates MMP2 protein in media. (Top): conditioned media from indicated cells & treatments. (Bottom): whole cell lysates were immunoblotted with indicated antibodies. (E) Recombinant WNT11 induces both MMP-2 protein & MMP-2 activity in media. (Top panels): Gelatin zymography & immunoblot of serum-free medium conditioned for the indicated times after recombinant WNT11 (r-WNT11) treatment. (Bottom): Whole cell lysates were immunoblotted with indicated antibodies. (F) MMP-2 inhibitor attenuated induced migration by WNT11. MDA-MB-231 cells infected with lentiviruses for stable expression of Wnt11 or GFP (n = 4) were incubated with either vehicle or 1 μM of ARP100. Media in the lower compartment had same concentration of DMSO or inhibitor. Values are mean ± s.e.m. *p " data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-134" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-134_rabbit-polyclonal-hif-1-alpha-antibody-310202415535194.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-134] -" data-caption="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-134] - ER-INP binds to PHD2, inhibits hydroxylation of HIF-1 alpha & increases HIF-1 alpha accumulation. (A) Co-immunoprecipitation assay. HEK293 cells were transfected with control vector or intrabody ER-INP for 48 hours & subsequently lysed. Co-immunoprecipitation & western blot assays were performed on the cell lysis. ER-INP recognized & bound to PHD2 in HEK293 cells (n = 3). (B) Western blot analysis to measure the effect of ER-INP on HIF-1 alpha & its hydroxylation level in transfected HEK293 & RAW264.7 cells pre-treated with MG132 (upper panel) & the protein ratio to beta-actin loading control by ImageJ densitometry analysis (lower panel; n = 3). (C) Immunofluorescence assay. Expression of ER-INP increases HIF-1 alpha accumulation in RAW264.7 cells. Cells were stained with anti-HIF-1 alpha antibody & DAPI & then visualized & photographed under immunofluorescence microscopy (left), & mean fluorescence intensity of HIF-1 alpha versus the mean fluorescence intensity of nuclear DAPI staining is displayed (right). Data represent the mean ± SD of 3 independent slides. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31413262), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-134" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-134_rabbit-polyclonal-hif-1-alpha-antibody-310202415541938.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-134] -" data-caption="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-134] - HIF-1 alpha binds to TIM-3 promoter & regulates its expression in primary glia.(a) Cell surface expression of TIM-3 analysed in BV2 cells under 20% O2 or 1% O2 for 24 h by flow cytometry using PE-conjugated anti-TIM-3 antibody. Results from 3 independent experiments presented as a representative histogram & the mean fold change (± s.d.) relative to normoxic sample. (b) Mouse primary mixed glial cells incubated under hypoxia or normoxia for 24 h, & the cells examined by immunocytochemistry using an anti-TIM-3 antibody. (c,d) Mouse primary mixed glial cells & primary neuronal cells incubated under hypoxia or normoxia for 24 h, & then RT–PCR used to detect the levels of TIM-3 & actin. Relative transcript levels shown as the mean fold change (± s.d.) from 3 independent experiments (NS, not significant, Student–Newman–Keuls test). (e) Primary mixed glial cells incubated under hypoxia or normoxia for 24 h, & chromatin immunoprecipitation (ChIP) performed w/ anti-HIF-1 alpha or control IgG. Results presented as relative amounts representative of 3 independent experiments. (f) Primary mixed glial cells cultured from HIF-1 alpha+f/+f mice, infected w/ Ad-GFP or Ad-Cre/GFP, transfected w/ TIM-3-luciferase reporter constructs & incubated under hypoxic or normoxic conditions for 24 h. Relative promoter activity is expressed as the ratio of luciferase activity/ beta-galactosidase activity. (g,h) RT–PCR (g) & WB analysis (h) performed under hypoxia or normoxia for 24 h using the indicated primers & antibodies, respectively. The data shown representative of at least 3 independent experiments. The graphs show the % changes in TIM-3 transcript & protein levels in Ad-Cre/GFP- versus Ad-GFP-infected cells under hypoxia. IP, immunoprecipitation. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25790768), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-134" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-134_rabbit-polyclonal-hif-1-alpha-antibody-31020241682181.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-134] -" data-caption="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-134] - Regulation of catabolic genes by HIF-1 alpha. (A) Protein levels of Hif1a, & mRNA levels of Mmp13, & Hif2a in WT mouse primary chondrocytes transduced with GFP or HIF1A adenoviral vectors under the hypoxic condition (3% O2). GFP or HIF1A was transduced at a multiplicity of infection (MOI) of 100. The cells were treated with or without 10 ng/mL IL-1 beta for 2 days. Bars show the mean ± SD of three samples per group. *P " data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-134" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-134_rabbit-polyclonal-hif-1-alpha-antibody-31020241554540.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-134] -" data-caption="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-134] - NSCs Reduce Succinate Levels & Reprogram the Metabolism of Type 1 Pro-inflammatory Mφ toward Oxidative Phosphorylation In Vitro(A) Experimental setup for in vitro MφLPS co-cultures with iNSCs/NSCs.(B & C) Gene expression microarrays of MφLPS-iNSCs/NSCs. (B) Venn diagram of differentially expressed genes (adjusted p value < 0.1). (C) Heatmap of genes differentially expressed (adjusted p value < 0.1) in MφLPS-iNSCs or MφLPS-NSCs.(D & E) qRT-PCR independent validation of differentially expressed inflammatory genes as in (C). (D) Expression of genes related to type 1 inflammatory (E) & anti-inflammatory Mφ phenotypes relative to Actb. Data are mean fold change (±SEM) versus MφLPS from n ≥ 3 independent replicates per condition.(F) qRT-PCR of BV2LPS-iNSCs/NSCs (±SEM) from n ≥ 3 independent experiments per condition. BV2 & BV2LPS are shown as controls.(G & H) Extracellular flux (XF) assay of the oxygen consumption rate (OCR) (G) & extracellular acidification rate (ECAR) (H) in MφLPS-iNSCs/NSCs. Data were normalized on total protein content & are expressed as mean values (±SEM) from n ≥ 3 independent experiments per condition.(I & J) Levels of significantly changed extracellular (EXTRA_Metab, I) & intracellular (INTRA_Metab, J) metabolites in MφLPS versus Mφ at 25 hr. Data are mean a.u. (±SEM) from n ≥ 2 independent experiments per condition.(K & L) Hif-1 alpha (K), PKM2 (K), & IL-1 beta (L) expression levels relative to beta-actin. Data are mean fold change versus MφLPS (±SEM) from n ≥ 3 independent experiments per condition.∗p ≤ 0.05 & ∗∗p ≤ 0.01 versus MφLPS. See also Tables S2 & S3. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29478844), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-134" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-134_rabbit-polyclonal-hif-1-alpha-antibody-310202415532035.jpg" data-alt="HIF-1 alpha Antibody - BSA Free" title="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-134] -" data-caption="Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-134] - Enhanced expression of hypoxia-induced survival factors & angiogenic growth factors in hASC L-spheroids.(A) The light source used was LED (660 nm) designed to fit over a microplate (12.5 × 8.5 cm) for cell culture. (B) Formation of hASC L-spheroids. hASCs morphology on non–tissue culture–treated 24-well plates at day 3. Scale bar = 500 μm. (C) Western blot analysis & quantification of HIF1-alpha in hASCs cultured as spheroids, L-spheroids & monolayers (*p < 0.01, compared to the L-spheroid group). (D) Angiogenesis-related protein analysis of L-spheroids (*, p < 0.05, compared to the spheroid group, t-test, n = 3 in each group). (E) ELISA measurement of spheroids cultured for 3 days. Concentrations of VEGF are presented as pg-corrected for 104 cells. (*, p < 0.05, compared with spheroid 6J/cm2 group, t-test, n = 3 in each group). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26065900), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody - BSA Free, Catalog # NB100-134" data-width="250" data-height="250" class="none"></i> </div> </div> </div> </div> <div class="atc-block-search atc-block-collapsible" data-type="search" data-blockid="ProductCart-NB100-134" > <form action="/commerce/addtocart" method="post" class="px-3 pt-3 add-to-cart-block" data-productcode="NB100-134"> <div class="commerce-atc-full-header atc-header col-four row border-bottom pb-1 m-0"> <div class="commerce-atc-catnum atc-head col-4 col-md-4 font-weight-bold">Catalog #</div> <div class="commerce-atc-availability atc-head col-md-3 font-weight-bold d-none d-md-block">Availability </div> <div class="commerce-atc-price atc-head col-5 col-md-3 font-weight-bold">Size / Price</div> <div class="commerce-atc-quantity atc-head col-3 col-md-2 text-right font-weight-bold">Qty</div> </div> <div class="spinner-border spinner-border-sm mt-3 ml-3" role="status"> <span class="sr-only">Loading...</span> </div> <div class="d-none search-product-mask"> <div id="ProductCart-NB100-134" class="atc-lines"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="NB100-134"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> NB100-134 </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="NB100-134SS"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> NB100-134SS </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> </div> <div class="atc-control ProductCart-NB100-134-control text-right"> <div class="row d-flex justify-content-end mx-0 p-0"> <div class="order-md-last m___button-atc mx-1"> <button type="submit" value="Add To Cart" class="atc-add-button btn a___button-atc m-1 col-xs-4 order-last btn-md-block w-100"> Add To Cart </button> </div> <div class="m___button-atc mx-1"> <a href="/services/bulk-quotes?product_code=NB100-134" class="atc-bulk-button btn a___button-secondary m-1 col-xs-4 btn-md-block w-100" rel="nofollow">Bulk Order</a> </div> </div> </div> </div> </form> </div> </div> </div> </div> <div class="search_result atc-container pb-3" data-brand="novus" data-category="Primary Antibodies" data-id="NBP2-75977" data-name="HIF-1 alpha Antibody (2443B) - BSA Free" data-position="4"> <div class="search_details border bg-white p-3 rounded"> <div class="row"> <div class="col-md-9"> <a href="/p/antibodies/hif-1-alpha-antibody-2443b_nbp2-75977" title="HIF-1 alpha Antibody (2443B) - BSA Free"> <h4>HIF-1 alpha Antibody (2443B) - BSA Free </h4> </a> </div> <div class="search_result_brand_logo col-md-3 mb-1 d-flex justify-content-xl-end product-brand-logo"> <img alt="Novus Biologicals, a Bio-Techne brand" src="https://resources.bio-techne.com/bio-techne-assets/images/logos/bio-techne-novus-biologicals.svg" class="d-flex w-75"> </div> </div> <div class="row"> <div class="col-md-9"> <div class="mb-1 d-inline-flex"> <div class="align-middle my-1 mr-3 d-block d-md-inline"> Catalog #: <a href="/p/antibodies/hif-1-alpha-antibody-2443b_nbp2-75977" title="HIF-1 alpha Antibody (2443B) - BSA Free"> NBP2-75977 </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold text-nowrap"> <a href="/p/antibodies/hif-1-alpha-antibody-2443b_nbp2-75977#product-documents"><span class="icon-icon-pdf text-error-red"></span> Product Documents</a> </div> <div class="d-none d-md-inline my-1"> <a href="#" class="search_result_images"><span class="icon-icon-image-camera-plus"></span> Images (5)</a> </div> </div> <div class="d-none d-md-block"> <span class="search_result_applications"></span><span class="applications_title"><strong>Applications:</strong></span> WB, ICC/IF, Simple Western, KO </div> <div class="d-none d-md-block"> <span class="species_title"><strong>Reactivity:</strong></span> Human </div> </div> <div class="col-md-3"> <button class="py-1 btn-default btn-block atc-view-more collapsed" data-toggle="collapse" href="#collapseButton-4" role="button" aria-expanded="false" aria-controls="collapseButton">View More</button> </div> </div> <div class="row"> <div class="align-middle mt-2 col-xs-12 col-sm-9"> <p class="mb-0">Recombinant Monoclonal Antibody.</p> </div> </div> <div class="commerce-atc-search pt-3 collapse" id="collapseButton-4"> <div class="row"> <div class="search_result_description col-xs-12 col-md-9"> <table class="tablesaw tablesaw-stack mb-2"> <tbody> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Reactivity:</strong></td> <td class="pb-1 w-75 text-break"> Human </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Details:</strong></td> <td class="pb-1 w-75 text-break"> Rabbit IgG Monoclonal Clone #2443B </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Applications:</strong></td> <td class="pb-1 w-75 text-break"> WB, ICC/IF, Simple Western, KO </td> </tr> </tbody> </table> <div class="compare_tool_select form-inline mb-2" data-sku="nbp2-75977"></div> </div> <div class="search_result_image_wrapper col-xs-12 col-md-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="novus"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src=https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-2443B-Western-Blot-NBP2-75977-img0002.jpg data-srcset=https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-2443B-Western-Blot-NBP2-75977-img0002.jpg alt="Western Blot: HIF-1 alpha Antibody (2443B)BSA Free [NBP2-75977]" title="Western Blot: HIF-1 alpha Antibody (2443B)BSA Free [NBP2-75977]" data-caption="Western Blot: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Total protein from Human U87 cells treated with or without CoCl2 and under Normoxic and Hypoxic conditions was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-HIF-1 alpha in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence." data-badges="<span class="biological-validation"></span>" data-application="Western Blot" data-header="HIF-1 alpha Antibody (2443B) - BSA Free, Catalog # NBP2-75977" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> <span class="image-count-text align-middle">(5)</span> </div> </div> </div> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-2443B-Knockdown-Validated-NBP2-75977-img0003.jpg" data-alt="Knockout Validated: HIF-1 alpha Antibody (2443B) - BSA Free [NBP2-75977]" title="Knockout Validated: HIF-1 alpha Antibody (2443B) - BSA Free [NBP2-75977]" data-caption="Knockout Validated: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HIF-1 alpha knockout HeLa cell line (KO) untreated (-) or treated (+) with 1 mM DFO for overnight. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-Human HIF-1 alpha Monoclonal Antibody (NBP2-75977) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HIF-1 alpha at an approximatel molecular weight of 120 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control.This experiment was conducted under reducing conditions." data-badges="<span class="knockout-validation"></span>" data-application="Knockout Validated" data-header="HIF-1 alpha Antibody (2443B) - BSA Free, Catalog # NBP2-75977" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-2443B-Immunocytochemistry-Immunofluorescence-NBP2-75977-img0001.jpg" data-alt="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody (2443B) - BSA Free [NBP2-75977]" title="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody (2443B) - BSA Free [NBP2-75977]" data-caption="Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Immunofluorescent staining of HIF-1 alpha using catalog number NBP2-75977 (clone 2443B). Staining was observed in Hela cells treated with DFO but not in untreated Hela cells." data-badges="<span class="biological-validation"></span>" data-application="Immunocytochemistry/ Immunofluorescence" data-header="HIF-1 alpha Antibody (2443B) - BSA Free, Catalog # NBP2-75977" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-2443B-Simple-Western-NBP2-75977-img0004.jpg" data-alt="Simple Western: HIF-1 alpha Antibody (2443B)BSA Free [NBP2-75977]" title="Simple Western: HIF-1 alpha Antibody (2443B)BSA Free [NBP2-75977]" data-caption="Simple Western: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Lane view shows a specific band for HIF-1 alpha in HepG2 +/- DFO cell lysate using 20ug/ml. Electropherogram image of corresponding Simple Western lane view at WES molecular weight of 116 KDa. Image from an internal validation." data-badges="" data-application="Simple Western" data-header="HIF-1 alpha Antibody (2443B) - BSA Free, Catalog # NBP2-75977" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-2443B-Simple-Western-NBP2-75977-img0005.jpg" data-alt="Simple Western: HIF-1 alpha Antibody (2443B)BSA Free [NBP2-75977]" title="Simple Western: HIF-1 alpha Antibody (2443B)BSA Free [NBP2-75977]" data-caption="Simple Western: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Simple Western lane view shows lysates of MCF +/- DMOG, BioSpherix MSCs in hypoxic conditions, and BioSpherix MSCs in normoxic conditions loaded at 0.5 mg/ml. A band was detected for HIF-1 alpha at approximately 116 kDa (as indicated) using NBP2-75977 (1:100 dilution) followed by Anti-Rabbit Secondary Antibody (042-206, ProteinSimple). This experiment was conducted under standard assay conditions, and using the 12-230 kDa separation module (SW-W004). Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody. Image from an internal validation." data-badges="" data-application="Simple Western" data-header="HIF-1 alpha Antibody (2443B) - BSA Free, Catalog # NBP2-75977" data-width="250" data-height="250" class="none"></i> </div> </div> </div> </div> <div class="atc-block-search atc-block-collapsible" data-type="search" data-blockid="ProductCart-NBP2-75977" > <form action="/commerce/addtocart" method="post" class="px-3 pt-3 add-to-cart-block" data-productcode="NBP2-75977"> <div class="commerce-atc-full-header atc-header col-four row border-bottom pb-1 m-0"> <div class="commerce-atc-catnum atc-head col-4 col-md-4 font-weight-bold">Catalog #</div> <div class="commerce-atc-availability atc-head col-md-3 font-weight-bold d-none d-md-block">Availability </div> <div class="commerce-atc-price atc-head col-5 col-md-3 font-weight-bold">Size / Price</div> <div class="commerce-atc-quantity atc-head col-3 col-md-2 text-right font-weight-bold">Qty</div> </div> <div class="spinner-border spinner-border-sm mt-3 ml-3" role="status"> <span class="sr-only">Loading...</span> </div> <div class="d-none search-product-mask"> <div id="ProductCart-NBP2-75977" class="atc-lines"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="NBP2-75977"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> NBP2-75977 </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="NBP2-75977SS"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> NBP2-75977SS </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> </div> <div class="atc-control ProductCart-NBP2-75977-control text-right"> <div class="row d-flex justify-content-end mx-0 p-0"> <div class="order-md-last m___button-atc mx-1"> <button type="submit" value="Add To Cart" class="atc-add-button btn a___button-atc m-1 col-xs-4 order-last btn-md-block w-100"> Add To Cart </button> </div> <div class="m___button-atc mx-1"> <a href="/services/bulk-quotes?product_code=NBP2-75977" class="atc-bulk-button btn a___button-secondary m-1 col-xs-4 btn-md-block w-100" rel="nofollow">Bulk Order</a> </div> </div> </div> </div> </form> </div> </div> </div> </div> <div class="search_result atc-container pb-3" data-brand="novus" data-category="Primary Antibodies" data-id="NBP2-75978" data-name="HIF-1 alpha Antibody (2443C) - BSA Free" data-position="5"> <div class="search_details border bg-white p-3 rounded"> <div class="row"> <div class="col-md-9"> <a href="/p/antibodies/hif-1-alpha-antibody-2443c_nbp2-75978" title="HIF-1 alpha Antibody (2443C) - BSA Free"> <h4>HIF-1 alpha Antibody (2443C) - BSA Free </h4> </a> </div> <div class="search_result_brand_logo col-md-3 mb-1 d-flex justify-content-xl-end product-brand-logo"> <img alt="Novus Biologicals, a Bio-Techne brand" src="https://resources.bio-techne.com/bio-techne-assets/images/logos/bio-techne-novus-biologicals.svg" class="d-flex w-75"> </div> </div> <div class="row"> <div class="col-md-9"> <div class="mb-1 d-inline-flex"> <div class="align-middle my-1 mr-3 d-block d-md-inline"> Catalog #: <a href="/p/antibodies/hif-1-alpha-antibody-2443c_nbp2-75978" title="HIF-1 alpha Antibody (2443C) - BSA Free"> NBP2-75978 </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold"> <a href="/p/antibodies/hif-1-alpha-antibody-2443c_nbp2-75978#reviews" class="searchImage_star5"> (2) </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold"> <a href="/p/antibodies/hif-1-alpha-antibody-2443c_nbp2-75978#citations"><span class="icon-icon-product-citations"></span>Citations (1)</a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold text-nowrap"> <a href="/p/antibodies/hif-1-alpha-antibody-2443c_nbp2-75978#product-documents"><span class="icon-icon-pdf text-error-red"></span> Product Documents</a> </div> <div class="d-none d-md-inline my-1"> <a href="#" class="search_result_images"><span class="icon-icon-image-camera-plus"></span> Images (6)</a> </div> </div> <div class="d-none d-md-block"> <span class="search_result_applications"></span><span class="applications_title"><strong>Applications:</strong></span> IHC, WB, ICC/IF, Simple Western, KO </div> <div class="d-none d-md-block"> <span class="species_title"><strong>Reactivity:</strong></span> Human </div> </div> <div class="col-md-3"> <button class="py-1 btn-default btn-block atc-view-more collapsed" data-toggle="collapse" href="#collapseButton-5" role="button" aria-expanded="false" aria-controls="collapseButton">View More</button> </div> </div> <div class="row"> <div class="align-middle mt-2 col-xs-12 col-sm-9"> <p class="mb-0">Recombinant Monoclonal Antibody.</p> </div> </div> <div class="commerce-atc-search pt-3 collapse" id="collapseButton-5"> <div class="row"> <div class="search_result_description col-xs-12 col-md-9"> <table class="tablesaw tablesaw-stack mb-2"> <tbody> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Reactivity:</strong></td> <td class="pb-1 w-75 text-break"> Human </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Details:</strong></td> <td class="pb-1 w-75 text-break"> Rabbit IgG Monoclonal Clone #2443C </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Applications:</strong></td> <td class="pb-1 w-75 text-break"> IHC, WB, ICC/IF, Simple Western, KO </td> </tr> </tbody> </table> <div class="compare_tool_select form-inline mb-2" data-sku="nbp2-75978"></div> </div> <div class="search_result_image_wrapper col-xs-12 col-md-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="novus"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src=https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-2443C-Western-Blot-NBP2-75978-img0004.jpg data-srcset=https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-2443C-Western-Blot-NBP2-75978-img0004.jpg alt="Western Blot: HIF-1 alpha Antibody (2443C)BSA Free [NBP2-75978]" title="Western Blot: HIF-1 alpha Antibody (2443C)BSA Free [NBP2-75978]" data-caption="Western Blot: HIF-1 alpha Antibody (2443C) [NBP2-75978] - Total protein from Human U87 cells treated with or without CoCl2 and under Normoxic and Hypoxic conditions was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-HIF-1 alpha in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence." data-badges="<span class="biological-validation"></span>" data-application="Western Blot" data-header="HIF-1 alpha Antibody (2443C) - BSA Free, Catalog # NBP2-75978" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> <span class="image-count-text align-middle">(6)</span> </div> </div> </div> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-2443C-Knockout-Validated-NBP2-75978-img0006.jpg" data-alt="Knockout Validated: HIF-1 alpha Antibody (2443C) - BSA Free [NBP2-75978]" title="Knockout Validated: HIF-1 alpha Antibody (2443C) - BSA Free [NBP2-75978]" data-caption="Knockout Validated: HIF-1 alpha Antibody (2443C) [NBP2-75978] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HIF-1 alpha knockout HeLa cell line (KO) untreated (-) or treated (+) with 1 mM DFO for overnight. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-Human HIF-1 alpha Monoclonal Antibody (Catalog # NBP2-75978) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HIF-1 alpha at approximately 120 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control." data-badges="<span class="knockout-validation"></span>" data-application="Knockout Validated" data-header="HIF-1 alpha Antibody (2443C) - BSA Free, Catalog # NBP2-75978" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-2443C-Immunocytochemistry-Immunofluorescence-NBP2-75978-img0002.jpg" data-alt="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody (2443C) - BSA Free [NBP2-75978]" title="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody (2443C) - BSA Free [NBP2-75978]" data-caption="Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody (2443C) [NBP2-75978] - Immunofluorescent staining of HIF-1 alpha using catalog number NBP2-75977 (clone 2443B). Staining was observed in Hela cells treated with DFO but not in untreated Hela cells." data-badges="<span class="biological-validation"></span>" data-application="Immunocytochemistry/ Immunofluorescence" data-header="HIF-1 alpha Antibody (2443C) - BSA Free, Catalog # NBP2-75978" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-2443C-Immunohistochemistry-Paraffin-NBP2-75978-img0003.jpg" data-alt="Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (2443C) - BSA Free [NBP2-75978]" title="Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (2443C) - BSA Free [NBP2-75978]" data-caption="Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (2443C) [NBP2-75978] - HIF-1 alpha (clone 2443C) was detected in immersion fixed paraffin-embedded sections of human kidney using Rabbit Anti-Human HIF-1 alpha Monoclonal Antibody (Catalog # NBP2-75978) at 1.0 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte (TM) HRP Polymer Antibody (Catalog # VC002). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the nuclei." data-badges="" data-application="Immunohistochemistry-Paraffin" data-header="HIF-1 alpha Antibody (2443C) - BSA Free, Catalog # NBP2-75978" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-2443C-Simple-Western-NBP2-75978-img0007.jpg" data-alt="Simple Western: HIF-1 alpha Antibody (2443C)BSA Free [NBP2-75978]" title="Simple Western: HIF-1 alpha Antibody (2443C)BSA Free [NBP2-75978]" data-caption="Simple Western: HIF-1 alpha Antibody (2443C) [NBP2-75978] - Lane view shows a specific band for HIF-1 alpha in HepG2 +/- DFO cell lysate using 20 ug/mL. Electropherogram image of corresponding Simple Western lane view at WES molecular weight of 116 KDa. Image from an internal validation." data-badges="" data-application="Simple Western" data-header="HIF-1 alpha Antibody (2443C) - BSA Free, Catalog # NBP2-75978" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-2443C-Simple-Western-NBP2-75978-img0008.jpg" data-alt="Simple Western: HIF-1 alpha Antibody (2443C)BSA Free [NBP2-75978]" title="Simple Western: HIF-1 alpha Antibody (2443C)BSA Free [NBP2-75978]" data-caption="Simple Western: HIF-1 alpha Antibody (2443C) [NBP2-75978] - Simple Western lane view shows lysates of MCF +/- DMOG, BioSpherix MSCs in hypoxic conditions, and BioSpherix MSCs in normoxic conditions loaded at 0.5 mg/ml. A band was detected for HIF-1 alpha at approximately 116 kDa (as indicated) using NBP2-75978 (1:100 dilution) followed by Anti-Rabbit Secondary Antibody (042-206, ProteinSimple). This experiment was conducted under standard assay conditions, and using the 12-230 kDa separation module (SW-W004). Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody. Image from an internal validation." data-badges="" data-application="Simple Western" data-header="HIF-1 alpha Antibody (2443C) - BSA Free, Catalog # NBP2-75978" data-width="250" data-height="250" class="none"></i> </div> </div> </div> </div> <div class="atc-block-search atc-block-collapsible" data-type="search" data-blockid="ProductCart-NBP2-75978" > <form action="/commerce/addtocart" method="post" class="px-3 pt-3 add-to-cart-block" data-productcode="NBP2-75978"> <div class="commerce-atc-full-header atc-header col-four row border-bottom pb-1 m-0"> <div class="commerce-atc-catnum atc-head col-4 col-md-4 font-weight-bold">Catalog #</div> <div class="commerce-atc-availability atc-head col-md-3 font-weight-bold d-none d-md-block">Availability </div> <div class="commerce-atc-price atc-head col-5 col-md-3 font-weight-bold">Size / Price</div> <div class="commerce-atc-quantity atc-head col-3 col-md-2 text-right font-weight-bold">Qty</div> </div> <div class="spinner-border spinner-border-sm mt-3 ml-3" role="status"> <span class="sr-only">Loading...</span> </div> <div class="d-none search-product-mask"> <div id="ProductCart-NBP2-75978" class="atc-lines"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="NBP2-75978"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> NBP2-75978 </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="NBP2-75978SS"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> NBP2-75978SS </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> </div> <div class="atc-control ProductCart-NBP2-75978-control text-right"> <div class="row d-flex justify-content-end mx-0 p-0"> <div class="order-md-last m___button-atc mx-1"> <button type="submit" value="Add To Cart" class="atc-add-button btn a___button-atc m-1 col-xs-4 order-last btn-md-block w-100"> Add To Cart </button> </div> <div class="m___button-atc mx-1"> <a href="/services/bulk-quotes?product_code=NBP2-75978" class="atc-bulk-button btn a___button-secondary m-1 col-xs-4 btn-md-block w-100" rel="nofollow">Bulk Order</a> </div> </div> </div> </div> </form> </div> </div> </div> </div> <div class="search_result atc-container pb-3" data-brand="rnd" data-category="Primary Antibodies" data-id="MAB1536" data-name="Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody" data-position="6"> <div class="search_details border bg-white p-3 rounded"> <div class="row"> <div class="col-md-9"> <a href="/p/antibodies/human-mouse-rat-hif-1-alpha-hif1a-antibody-241809_mab1536" title="Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody"> <h4>Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody </h4> </a> </div> <div class="search_result_brand_logo col-md-3 mb-1 d-flex justify-content-xl-end product-brand-logo"> <img alt="R&D Systems, a Bio-Techne brand" src="https://resources.bio-techne.com/bio-techne-assets/images/logos/bio-techne-rnd-systems.svg" class="d-flex w-75"> </div> </div> <div class="row"> <div class="col-md-9"> <div class="mb-1 d-inline-flex"> <div class="align-middle my-1 mr-3 d-block d-md-inline"> Catalog #: <a href="/p/antibodies/human-mouse-rat-hif-1-alpha-hif1a-antibody-241809_mab1536" title="Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody"> MAB1536 </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold"> <a href="/p/antibodies/human-mouse-rat-hif-1-alpha-hif1a-antibody-241809_mab1536#reviews" class="searchImage_star5"> (2) </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold"> <a href="/p/antibodies/human-mouse-rat-hif-1-alpha-hif1a-antibody-241809_mab1536#citations"><span class="icon-icon-product-citations"></span>Citations (88)</a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold text-nowrap"> <a href="/p/antibodies/human-mouse-rat-hif-1-alpha-hif1a-antibody-241809_mab1536#product-documents"><span class="icon-icon-pdf text-error-red"></span> Product Documents</a> </div> <div class="d-none d-md-inline my-1"> <a href="#" class="search_result_images"><span class="icon-icon-image-camera-plus"></span> Images (32)</a> </div> </div> <div class="d-none d-md-block"> <span class="search_result_applications"></span><span class="applications_title"><strong>Applications:</strong></span> WB, Simple Western, IHC, IP, KO </div> <div class="d-none d-md-block"> <span class="species_title"><strong>Reactivity:</strong></span> Human, Mouse, Rat </div> </div> <div class="col-md-3"> <button class="py-1 btn-default btn-block atc-view-more collapsed" data-toggle="collapse" href="#collapseButton-6" role="button" aria-expanded="false" aria-controls="collapseButton">View More</button> </div> </div> <div class="commerce-atc-search pt-3 collapse" id="collapseButton-6"> <div class="row"> <div class="search_result_description col-xs-12 col-md-9"> <table class="tablesaw tablesaw-stack mb-2"> <tbody> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Reactivity:</strong></td> <td class="pb-1 w-75 text-break"> Human, Mouse, Rat </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Details:</strong></td> <td class="pb-1 w-75 text-break"> Mouse IgG1 Monoclonal Clone #241809 </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Applications:</strong></td> <td class="pb-1 w-75 text-break"> WB, Simple Western, IHC, IP, KO </td> </tr> </tbody> </table> <div class="compare_tool_select form-inline mb-2" data-sku="mab1536"></div> </div> <div class="search_result_image_wrapper col-xs-12 col-md-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="rnd"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src=https://resources.bio-techne.com/images/products/antibody/HIF-1_alpha_MAB1536_Western_Blot_5973.jpg data-srcset=https://resources.bio-techne.com/images/products/antibody/HIF-1_alpha_MAB1536_Western_Blot_5973.jpg alt="Detection of Human, Mouse, and Rat HIF-1 alpha/HIF1A antibody by Western Blot." title="Detection of Human, Mouse, and Rat HIF-1 alpha/HIF1A by Western Blot." data-caption="Western blot shows lysates of MCF-7 human breast cancer cell line, Balb-3T3 mouse embryonic fibroblast cell line, and PC-12 rat adrenal pheochromocytoma cell line untreated (-) or treated (+) with 150 µM CoCl<sub>2</sub>for 8 hours. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat HIF-1 alpha/HIF1A Monoclonal Antibody, followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for HIF-1 alpha/HIF1A at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." data-badges="<span class="biological-validation"></span>" data-application="Western Blot" data-header="Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody, Catalog # MAB1536" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> <span class="image-count-text align-middle">(32)</span> </div> </div> </div> <i data-src="https://resources.bio-techne.com/images/products/antibody/HIF1_alpha_MAB1536_Immunohistochemistry_23169.jpg" data-alt="HIF-1 alpha/HIF1A antibody in Human Kidney by Immunohistochemistry (IHC-P)." title="HIF-1 alpha/HIF1A in Human Kidney." data-caption="HIF-1 alpha/HIF1A was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human/Mouse/Rat HIF-1 alpha/HIF1A Monoclonal Antibody (Catalog # MAB1536) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." data-badges="" data-application="Immunohistochemistry" data-header="Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody, Catalog # MAB1536" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/antibody/HIF1_alpha_MAB1536_Knockout_Validated_21972.jpg" data-alt="Western Blot Shows Human HIF-1 alpha/HIF1A alpha/HIF1A Antibody Specificity by Using Knockout Cell Line." title="Western Blot Shows Human HIF-1 alpha/HIF1A alpha/HIF1A Specificity by Using Knockout Cell Line." data-caption="Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HIF-1 alpha/HIF1A knockout HeLa cell line (KO) untreated (-) or treated (+) with 1 mM DFO overnight. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat HIF-1 alpha/HIF1A Monoclonal Antibody (Catalog # MAB1536) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HIF-1 alpha/HIF1A at approximately 110 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." data-badges="<span class="knockout-validation"></span>" data-application="Knockout Validated" data-header="Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody, Catalog # MAB1536" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/antibody/16314.jpg" data-alt="Detection of Human HIF-1 alpha/HIF1A antibody by Simple WesternTM." title="Detection of Human HIF-1 alpha/HIF1A by Simple Western<sup>TM</sup>." data-caption="Simple Western lane view shows lysates of A549 human lung carcinoma cell line untreated (-) or treated (+) with Hypoxia (1% O<sub>2</sub>), loaded at 0.2 mg/mL. A specific band was detected for HIF-1 alpha/HIF1A at approximately 116 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse/Rat HIF-1 alpha/HIF1A Monoclonal Antibody (Catalog # MAB1536). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." data-badges="<span class="biological-validation"></span>" data-application="Simple Western" data-header="Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody, Catalog # MAB1536" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/antibody/HIF1_alpha_MAB1536_Knockout_Validated_22419.jpg" data-alt="HIF-1 alpha/HIF1A Antibody Specificity is Shown by Immunocytochemistry in Knockout Cell Line." title="HIF-1 alpha/HIF1A Specificity is Shown by Immunocytochemistry in Knockout Cell Line." data-caption="HIF-1 alpha/HIF1A was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line treated with DFO but is not detected in HIF-1 alpha/HIF1A knockout (KO) HeLa Human Cell Line cell line using Mouse Anti-Human/Mouse/Rat HIF-1 alpha/HIF1A alpha/HIF1A Monoclonal Antibody (Catalog # MAB1536) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." data-badges="<span class="knockout-validation"></span>" data-application="Knockout Validated" data-header="Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody, Catalog # MAB1536" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/mab1536_human-mouse-rat-hif-1-alpha-mab-clone-241809-41202410321413.jpg" data-alt="Detection of Human HIF-1 alpha by Western Blot" title="Detection of Human HIF-1 alpha by Western Blot" data-caption="Ubc13 activity is necessary for HIF1 alpha nuclear retention. (A) ChIP assays using Control IgG and HIF1 alpha antibodies were performed on cell extracts from U2OS cells pre-treated with 2μM NSC697923 for 30 min before being exposed to 1% O2 for 3 h. Immunoprecipitated DNA was analysed by qPCR using the indicated primer pairs and normalised to input genomic DNA. (B and C) U2OS cells were transfected with HA-HIF1 alpha (P402A, P564A) or empty vector and treated with 2 μM NSC697923 for 3 h. Whole cell lysates (B) or nuclear and cytoplasmic extracts (C) were prepared. Extracts were analysed by immunoblotting using the indicated antibodies. (D) Cytoplasmic and nuclear extracts were prepared from U2OS cells treated with 2 μM NSC697923 and 5 ng/ml Leptomycin B as indicated before being exposed to 1% O2 for 3 h. P-values are significant according to the Student's t-test; *P < 0.05, **P < 0.01. Image collected and cropped by CiteAb from the following publication (https://academic.oup.com/nar/article/45/16/9336/3896344), licensed under a CC-BY license. Not internally tested by R&D Systems." data-badges="<span class="biological-validation"></span>" data-application="Western Blot" data-header="Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody, Catalog # MAB1536" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/mab1536_human-mouse-rat-hif-1-alpha-mab-clone-241809-41202410333048.jpg" data-alt="Detection of Human HIF-1 alpha by Western Blot" title="Detection of Human HIF-1 alpha by Western Blot" data-caption="Increasing doses of Allopurinol reduce HIF-1 alpha levels in normoxic HFFs and HUVEC cells.A. Characterisation of HFF and HUVEC response to hypoxia. Cells were exposed to hypoxia (1% oxygen) for the indicated periods of time. At the end of incubation, protein levels were determined in whole cell extracts by immunoblot analysis using the depicted antibodies. B. Cells were treated with Allopurinol at 10, 100 and 1000 μg/ml for 17 hours. Then the cells were lysed for assessment of the indicated protein levels. Cells were treated with Etoposide (Etop) for 24 hours under normoxia. H-cells exposed to 1% O2 for 16 hours. HIF-1 alpha levels were quantified using ImageJ software and graph depicts mean and standard deviation of a minimum of three independent experiments. Anova t-test was performed and p values calculated as follows: *p" data-badges="<span class="biological-validation"></span>" data-application="Western Blot" data-header="Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody, Catalog # MAB1536" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/mab1536_human-mouse-rat-hif-1-alpha-mab-clone-241809-41202410331932.jpg" data-alt="Detection of Human HIF-1 alpha by Western Blot" title="Detection of Human HIF-1 alpha by Western Blot" data-caption="Increasing doses of Allopurinol reduce HIF-1 alpha levels in normoxic HFFs and HUVEC cells.A. Characterisation of HFF and HUVEC response to hypoxia. Cells were exposed to hypoxia (1% oxygen) for the indicated periods of time. At the end of incubation, protein levels were determined in whole cell extracts by immunoblot analysis using the depicted antibodies. B. Cells were treated with Allopurinol at 10, 100 and 1000 μg/ml for 17 hours. Then the cells were lysed for assessment of the indicated protein levels. Cells were treated with Etoposide (Etop) for 24 hours under normoxia. H-cells exposed to 1% O2 for 16 hours. HIF-1 alpha levels were quantified using ImageJ software and graph depicts mean and standard deviation of a minimum of three independent experiments. Anova t-test was performed and p values calculated as follows: *p" data-badges="<span class="biological-validation"></span>" data-application="Western Blot" data-header="Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody, Catalog # MAB1536" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/mab1536_human-mouse-rat-hif-1-alpha-mab-clone-241809-41202410333047.jpg" data-alt="Detection of Human HIF-1 alpha by Western Blot" title="Detection of Human HIF-1 alpha by Western Blot" data-caption="NQO1 enhances HIF-1 alpha expression.(a,b) RKO cells (a) and MDA-MB-231 cells (b) were exposed to 0.5% O2 for 8 h and harvested at the indicated times. Whole-cell lysates were analysed by immunoblotting for HIF-1 alpha, HIF-1 beta, NQO1 and beta-actin. SE, short exposure; LE, long exposure. (c,d) Various concentrations of pshNQO1 and pNQO1-myc-His6 (0–10 μg) were transiently transfected into RKO (c) and MDA-MB-231 (d) cells, respectively. After 48 h, the cells were exposed to 20 or 0.5% O2 for another 2 h, and then harvested. Whole-cell lysates were analysed by immunoblotting for HIF-1 alpha, NQO1 and beta-actin. EV indicates pshCont (c) and pCDNA3.1-myc-His6 (d). SE, short exposure; LE, long exposure. (e) pNQO1-myc-His6 was transiently transfected into RKO/pshNQO1 cells. After 48 h, the cells were exposed to 0.5% O2 for 2 h, and then harvested. Whole-cell lysates were analysed by immunoblotting for HIF-1 alpha, NQO1 and beta-actin. SE, short exposure; LE, long exposure. (f) Mouse embryonic fibroblast wild type and NQO1−/− cells were exposed to 20 or 0.5% O2 for 2 h and then harvested. Whole-cell lysates were analysed by immunoblotting for HIF-1 alpha, NQO1 and beta-actin. SE, short exposure; LE, long exposure. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27966538), licensed under a CC-BY license. Not internally tested by R&D Systems." data-badges="" data-application="Western Blot" data-header="Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody, Catalog # MAB1536" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/mab1536_human-mouse-rat-hif-1-alpha-mab-clone-241809-4120241032551.jpg" data-alt="Detection of Human HIF-1 alpha by Immunohistochemistry" title="Detection of Human HIF-1 alpha by Immunohistochemistry" data-caption="Upregulation of NQO1 correlates with poor prognosis and expression of HIF-1 alpha in colorectal cancer.(a) An Oncomine analysis of the TCGA colorectal database indicated that NQO1 expressions are elevated in colorectal cancers (n=102) compared with normal colorectal tissues (n=19). ****P" data-badges="" data-application="Immunohistochemistry" data-header="Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody, Catalog # MAB1536" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/mab1536_human-mouse-rat-hif-1-alpha-mab-clone-241809-41202410331971.jpg" data-alt="Detection of Human HIF-1 alpha by Western Blot" title="Detection of Human HIF-1 alpha by Western Blot" data-caption="Allopurinol reduces HIF-1 alpha levels independent of PHD function.A. Cells were pre-treated with Allopurinol at 10 and 1000 μg/ml for 60 minutes and then were incubated in 1% oxygen or treated with 200 μM DFX for 16 hours. Whole cell lysates were analysed by immunoblot using the indicated antibodies. B. Cells were pre-treated with Allopurinol at 1000 μg/ml for 60 minutes and then were incubated with 20μM MG132 for 3 hours. Whole cell lysates were analysed by immunoblot using the indicated antibodies. HIF-1 alpha levels were quantified using ImageJ software and graph depicts mean and standard deviation of a minimum of three independent experiments. Anova t-test was performed and p values calculated as follows: *p" data-badges="<span class="biological-validation"></span>" data-application="Western Blot" data-header="Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody, Catalog # MAB1536" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/mab1536_human-mouse-rat-hif-1-alpha-mab-clone-241809-41202410333043.jpg" data-alt="Detection of Human HIF-1 alpha by Western Blot" title="Detection of Human HIF-1 alpha by Western Blot" data-caption="SFN inhibits HIF-1 alpha protein accumulation.Cell lines were treated as described below, and total cell lysates subjected to immunoblot analysis. A, A549 cells were pretreated with or without SFN and MG132 (20 µM) for 1 hour. B, MCF7 cells were pretreated for 1 hour with indicated concentration of SFN, and then treated with 100µM cobalt chloride (Co+2) for an additional 5.5hours. C, 4T1 cells were pretreated for 20 min with 20 µM SFN, followed by treatment with 250µM cobalt chloride or 200µM desferroxamine as indicated for 4hours. D. 293 cells were pretreated for 20 min with indicated concentrations of SFN, followed by stimulation with 250 µM cobalt chloride for an additional 4 hours. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/23166763), licensed under a CC-BY license. Not internally tested by R&D Systems." data-badges="<span class="biological-validation"></span>" data-application="Western Blot" data-header="Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody, Catalog # MAB1536" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/mab1536_human-mouse-rat-hif-1-alpha-mab-clone-241809-4120241033190.jpg" data-alt="Detection of Human HIF-1 alpha by Western Blot" title="Detection of Human HIF-1 alpha by Western Blot" data-caption="XIAP ubiquitinates HIF1 alpha. (A) U2OS cells were transfected with siRNA oligonucleotides and His-tagged ubiquitin as indicated. Lysates were prepared and precipitated using nickel-conjugated beads followed by western blotting using the indicated antibodies. (B) HA-tagged plasmid encoding HIF1 alpha was coexpressed in HEK293 cells with His-tagged ubiquitin (Ub), FLAG-XIAP (X) or FLAG-VHL (V) as indicated. Lysates were prepared 48 h post-transfection and ubiquitinated material was recovered by incubation with nickel-conjugated beads and analysed by western blotting. (C) Biotinylated-HIF1 alpha was coexpressed in HEK293 cells with FLAG-tagged XIAP. HIF1 alpha was recovered from lysates by incubation with streptavidin-coated beads and analysed using antibodies directed against specific ubiquitin chains. (D) U2OS cells were pre-treated with 2μM NSC697923 for 30 min before being exposed to 1% O2 for 3 h. Cells were lysed in the presence of UBQ-TUBEs. Ubiquitin conjugates were purified using NiNTA agarose beads and analysed by immunoblotting using the indicated antibodies. (E) U2OS cells were pre-treated with 2 μM NSC697923 for 30 min before being exposed to 1% O2 for 3 h. Cells were lysed in the presence of TAB2-TUBEs or TAB2-AA-TUBEs as indicated. Proteins conjugated to Lys63-linked ubiquitin chains were purified using Ni-NTA agarose beads and analysed by immunoblot. Image collected and cropped by CiteAb from the following publication (https://academic.oup.com/nar/article/45/16/9336/3896344), licensed under a CC-BY license. Not internally tested by R&D Systems." data-badges="<span class="biological-validation"></span>" data-application="Western Blot" data-header="Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody, Catalog # MAB1536" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/mab1536_human-mouse-rat-hif-1-alpha-mab-clone-241809-41202410325541.jpg" data-alt="Detection of Human HIF-1 alpha by Western Blot" title="Detection of Human HIF-1 alpha by Western Blot" data-caption="Allopurinol reduces HIF-1 alpha levels independent of PHD function.A. Cells were pre-treated with Allopurinol at 10 and 1000 μg/ml for 60 minutes and then were incubated in 1% oxygen or treated with 200 μM DFX for 16 hours. Whole cell lysates were analysed by immunoblot using the indicated antibodies. B. Cells were pre-treated with Allopurinol at 1000 μg/ml for 60 minutes and then were incubated with 20μM MG132 for 3 hours. Whole cell lysates were analysed by immunoblot using the indicated antibodies. HIF-1 alpha levels were quantified using ImageJ software and graph depicts mean and standard deviation of a minimum of three independent experiments. Anova t-test was performed and p values calculated as follows: *p" data-badges="<span class="biological-validation"></span>" data-application="Western Blot" data-header="Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody, Catalog # MAB1536" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/mab1536_human-mouse-rat-hif-1-alpha-mab-clone-241809-41202410321423.jpg" data-alt="Detection of Human HIF-1 alpha by Western Blot" title="Detection of Human HIF-1 alpha by Western Blot" data-caption="XIAP ubiquitinates HIF1 alpha. (A) U2OS cells were transfected with siRNA oligonucleotides and His-tagged ubiquitin as indicated. Lysates were prepared and precipitated using nickel-conjugated beads followed by western blotting using the indicated antibodies. (B) HA-tagged plasmid encoding HIF1 alpha was coexpressed in HEK293 cells with His-tagged ubiquitin (Ub), FLAG-XIAP (X) or FLAG-VHL (V) as indicated. Lysates were prepared 48 h post-transfection and ubiquitinated material was recovered by incubation with nickel-conjugated beads and analysed by western blotting. (C) Biotinylated-HIF1 alpha was coexpressed in HEK293 cells with FLAG-tagged XIAP. HIF1 alpha was recovered from lysates by incubation with streptavidin-coated beads and analysed using antibodies directed against specific ubiquitin chains. (D) U2OS cells were pre-treated with 2μM NSC697923 for 30 min before being exposed to 1% O2 for 3 h. Cells were lysed in the presence of UBQ-TUBEs. Ubiquitin conjugates were purified using NiNTA agarose beads and analysed by immunoblotting using the indicated antibodies. (E) U2OS cells were pre-treated with 2 μM NSC697923 for 30 min before being exposed to 1% O2 for 3 h. Cells were lysed in the presence of TAB2-TUBEs or TAB2-AA-TUBEs as indicated. Proteins conjugated to Lys63-linked ubiquitin chains were purified using Ni-NTA agarose beads and analysed by immunoblot. Image collected and cropped by CiteAb from the following publication (https://academic.oup.com/nar/article/45/16/9336/3896344), licensed under a CC-BY license. Not internally tested by R&D Systems." data-badges="<span class="biological-validation"></span>" data-application="Western Blot" data-header="Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody, Catalog # MAB1536" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/mab1536_human-mouse-rat-hif-1-alpha-mab-clone-241809-41202410325565.jpg" data-alt="Detection of Human HIF-1 alpha by Western Blot" title="Detection of Human HIF-1 alpha by Western Blot" data-caption="SFN inhibits HIF-1 alpha protein accumulation.Cell lines were treated as described below, and total cell lysates subjected to immunoblot analysis. A, A549 cells were pretreated with or without SFN and MG132 (20 µM) for 1 hour. B, MCF7 cells were pretreated for 1 hour with indicated concentration of SFN, and then treated with 100µM cobalt chloride (Co+2) for an additional 5.5hours. C, 4T1 cells were pretreated for 20 min with 20 µM SFN, followed by treatment with 250µM cobalt chloride or 200µM desferroxamine as indicated for 4hours. D. 293 cells were pretreated for 20 min with indicated concentrations of SFN, followed by stimulation with 250 µM cobalt chloride for an additional 4 hours. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/23166763), licensed under a CC-BY license. Not internally tested by R&D Systems." data-badges="<span class="biological-validation"></span>" data-application="Western Blot" data-header="Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody, Catalog # MAB1536" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/mab1536_human-mouse-rat-hif-1-alpha-mab-clone-241809-4120241033817.jpg" data-alt="Detection of Human HIF-1 alpha by Western Blot" title="Detection of Human HIF-1 alpha by Western Blot" data-caption="NQO1 enhances HIF-1 alpha expression.(a,b) RKO cells (a) and MDA-MB-231 cells (b) were exposed to 0.5% O2 for 8 h and harvested at the indicated times. Whole-cell lysates were analysed by immunoblotting for HIF-1 alpha, HIF-1 beta, NQO1 and beta-actin. SE, short exposure; LE, long exposure. (c,d) Various concentrations of pshNQO1 and pNQO1-myc-His6 (0–10 μg) were transiently transfected into RKO (c) and MDA-MB-231 (d) cells, respectively. After 48 h, the cells were exposed to 20 or 0.5% O2 for another 2 h, and then harvested. Whole-cell lysates were analysed by immunoblotting for HIF-1 alpha, NQO1 and beta-actin. EV indicates pshCont (c) and pCDNA3.1-myc-His6 (d). SE, short exposure; LE, long exposure. (e) pNQO1-myc-His6 was transiently transfected into RKO/pshNQO1 cells. After 48 h, the cells were exposed to 0.5% O2 for 2 h, and then harvested. Whole-cell lysates were analysed by immunoblotting for HIF-1 alpha, NQO1 and beta-actin. SE, short exposure; LE, long exposure. (f) Mouse embryonic fibroblast wild type and NQO1−/− cells were exposed to 20 or 0.5% O2 for 2 h and then harvested. Whole-cell lysates were analysed by immunoblotting for HIF-1 alpha, NQO1 and beta-actin. SE, short exposure; LE, long exposure. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27966538), licensed under a CC-BY license. Not internally tested by R&D Systems." data-badges="" data-application="Western Blot" data-header="Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody, Catalog # MAB1536" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/mab1536_human-mouse-rat-hif-1-alpha-mab-clone-241809-41202410331939.jpg" data-alt="Detection of Human HIF-1 alpha by Western Blot" title="Detection of Human HIF-1 alpha by Western Blot" data-caption="SFN suppresses the HIF-1–mPGES –PGE2 axis by control of HIF-1 alpha protein expression without altering mRNA levels.A, A549 cells were pretreated with or without SFN for 30 minutes. IL1 beta (1 ng/ml) was then added and cells were cultured for another 4 hours. Total RNA was analyzed by quantitative RT-PCR. The results are expressed as fold change relative to untreated cells (mean ± SEM; n = 3). B&C, A549 cells were pretreated with or without SFN for 30 minutes. IL1 beta (1 ng/ml) was then added or not and cells were cultured for another 4 hours. Total cell lysates were subjected to immunoblot analysis (panel B) and total RNA was analyzed by quantitative RT-PCR (panel C). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/23166763), licensed under a CC-BY license. Not internally tested by R&D Systems." data-badges="<span class="biological-validation"></span>" data-application="Western Blot" data-header="Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody, Catalog # MAB1536" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/mab1536_human-mouse-rat-hif-1-alpha-mab-clone-241809-41202410325566.jpg" data-alt="Detection of Human HIF-1 alpha by Western Blot" title="Detection of Human HIF-1 alpha by Western Blot" data-caption="Ubc13 activity is necessary for HIF1 alpha nuclear retention. (A) ChIP assays using Control IgG and HIF1 alpha antibodies were performed on cell extracts from U2OS cells pre-treated with 2μM NSC697923 for 30 min before being exposed to 1% O2 for 3 h. Immunoprecipitated DNA was analysed by qPCR using the indicated primer pairs and normalised to input genomic DNA. (B and C) U2OS cells were transfected with HA-HIF1 alpha (P402A, P564A) or empty vector and treated with 2 μM NSC697923 for 3 h. Whole cell lysates (B) or nuclear and cytoplasmic extracts (C) were prepared. Extracts were analysed by immunoblotting using the indicated antibodies. (D) Cytoplasmic and nuclear extracts were prepared from U2OS cells treated with 2 μM NSC697923 and 5 ng/ml Leptomycin B as indicated before being exposed to 1% O2 for 3 h. P-values are significant according to the Student's t-test; *P < 0.05, **P < 0.01. Image collected and cropped by CiteAb from the following publication (https://academic.oup.com/nar/article/45/16/9336/3896344), licensed under a CC-BY license. Not internally tested by R&D Systems." data-badges="<span class="biological-validation"></span>" data-application="Western Blot" data-header="Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody, Catalog # MAB1536" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/mab1536_human-mouse-rat-hif-1-alpha-mab-clone-241809-41202410325525.jpg" data-alt="Detection of Human HIF-1 alpha by Western Blot" title="Detection of Human HIF-1 alpha by Western Blot" data-caption="SFN inhibits HIF-1 alpha protein accumulation.Cell lines were treated as described below, and total cell lysates subjected to immunoblot analysis. A, A549 cells were pretreated with or without SFN and MG132 (20 µM) for 1 hour. B, MCF7 cells were pretreated for 1 hour with indicated concentration of SFN, and then treated with 100µM cobalt chloride (Co+2) for an additional 5.5hours. C, 4T1 cells were pretreated for 20 min with 20 µM SFN, followed by treatment with 250µM cobalt chloride or 200µM desferroxamine as indicated for 4hours. D. 293 cells were pretreated for 20 min with indicated concentrations of SFN, followed by stimulation with 250 µM cobalt chloride for an additional 4 hours. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/23166763), licensed under a CC-BY license. Not internally tested by R&D Systems." data-badges="<span class="biological-validation"></span>" data-application="Western Blot" data-header="Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody, Catalog # MAB1536" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/mab1536_human-mouse-rat-hif-1-alpha-mab-clone-241809-41202410331928.jpg" data-alt="Detection of Mouse HIF-1 alpha by Western Blot" title="Detection of Mouse HIF-1 alpha by Western Blot" data-caption="SFN suppresses mPGES expression and PGE2 production driven by overexpression of HIF1 alpha.A549 cells were transfected with indicated amounts of HIF-1 alpha and GFP expression vectors. The total amount of transfected DNA (4 µg) was kept constant by addition of the empty vector. The cells were then left untreated or treated with increasing concentrations of SFN for 40 hours. The total cell extracts were prepared for immunoblot analysis to evaluate protein levels. The cell culture media were used for measuring PGE2 concentration by EIA assay. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/23166763), licensed under a CC-BY license. Not internally tested by R&D Systems." data-badges="<span class="biological-validation"></span>" data-application="Western Blot" data-header="Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody, Catalog # MAB1536" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/mab1536_human-mouse-rat-hif-1-alpha-mab-clone-241809-41202410325544.jpg" data-alt="Detection of Human HIF-1 alpha by Western Blot" title="Detection of Human HIF-1 alpha by Western Blot" data-caption="NQO1 enhances HIF-1 alpha expression.(a,b) RKO cells (a) and MDA-MB-231 cells (b) were exposed to 0.5% O2 for 8 h and harvested at the indicated times. Whole-cell lysates were analysed by immunoblotting for HIF-1 alpha, HIF-1 beta, NQO1 and beta-actin. SE, short exposure; LE, long exposure. (c,d) Various concentrations of pshNQO1 and pNQO1-myc-His6 (0–10 μg) were transiently transfected into RKO (c) and MDA-MB-231 (d) cells, respectively. After 48 h, the cells were exposed to 20 or 0.5% O2 for another 2 h, and then harvested. Whole-cell lysates were analysed by immunoblotting for HIF-1 alpha, NQO1 and beta-actin. EV indicates pshCont (c) and pCDNA3.1-myc-His6 (d). SE, short exposure; LE, long exposure. (e) pNQO1-myc-His6 was transiently transfected into RKO/pshNQO1 cells. After 48 h, the cells were exposed to 0.5% O2 for 2 h, and then harvested. Whole-cell lysates were analysed by immunoblotting for HIF-1 alpha, NQO1 and beta-actin. SE, short exposure; LE, long exposure. (f) Mouse embryonic fibroblast wild type and NQO1−/− cells were exposed to 20 or 0.5% O2 for 2 h and then harvested. Whole-cell lysates were analysed by immunoblotting for HIF-1 alpha, NQO1 and beta-actin. SE, short exposure; LE, long exposure. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27966538), licensed under a CC-BY license. Not internally tested by R&D Systems." data-badges="" data-application="Western Blot" data-header="Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody, Catalog # MAB1536" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/mab1536_human-mouse-rat-hif-1-alpha-mab-clone-241809-41202410333010.jpg" data-alt="Detection of Mouse HIF-1 alpha by Western Blot" title="Detection of Mouse HIF-1 alpha by Western Blot" data-caption="SFN inhibits HIF-1 alpha protein accumulation.Cell lines were treated as described below, and total cell lysates subjected to immunoblot analysis. A, A549 cells were pretreated with or without SFN and MG132 (20 µM) for 1 hour. B, MCF7 cells were pretreated for 1 hour with indicated concentration of SFN, and then treated with 100µM cobalt chloride (Co+2) for an additional 5.5hours. C, 4T1 cells were pretreated for 20 min with 20 µM SFN, followed by treatment with 250µM cobalt chloride or 200µM desferroxamine as indicated for 4hours. D. 293 cells were pretreated for 20 min with indicated concentrations of SFN, followed by stimulation with 250 µM cobalt chloride for an additional 4 hours. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/23166763), licensed under a CC-BY license. Not internally tested by R&D Systems." data-badges="<span class="biological-validation"></span>" data-application="Western Blot" data-header="Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody, Catalog # MAB1536" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/mab1536_human-mouse-rat-hif-1-alpha-mab-clone-241809-4120241033813.jpg" data-alt="Detection of Human HIF-1 alpha by Western Blot" title="Detection of Human HIF-1 alpha by Western Blot" data-caption="NQO1 enhances HIF-1 alpha expression.(a,b) RKO cells (a) and MDA-MB-231 cells (b) were exposed to 0.5% O2 for 8 h and harvested at the indicated times. Whole-cell lysates were analysed by immunoblotting for HIF-1 alpha, HIF-1 beta, NQO1 and beta-actin. SE, short exposure; LE, long exposure. (c,d) Various concentrations of pshNQO1 and pNQO1-myc-His6 (0–10 μg) were transiently transfected into RKO (c) and MDA-MB-231 (d) cells, respectively. After 48 h, the cells were exposed to 20 or 0.5% O2 for another 2 h, and then harvested. Whole-cell lysates were analysed by immunoblotting for HIF-1 alpha, NQO1 and beta-actin. EV indicates pshCont (c) and pCDNA3.1-myc-His6 (d). SE, short exposure; LE, long exposure. (e) pNQO1-myc-His6 was transiently transfected into RKO/pshNQO1 cells. After 48 h, the cells were exposed to 0.5% O2 for 2 h, and then harvested. Whole-cell lysates were analysed by immunoblotting for HIF-1 alpha, NQO1 and beta-actin. SE, short exposure; LE, long exposure. (f) Mouse embryonic fibroblast wild type and NQO1−/− cells were exposed to 20 or 0.5% O2 for 2 h and then harvested. Whole-cell lysates were analysed by immunoblotting for HIF-1 alpha, NQO1 and beta-actin. SE, short exposure; LE, long exposure. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27966538), licensed under a CC-BY license. Not internally tested by R&D Systems." data-badges="" data-application="Western Blot" data-header="Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody, Catalog # MAB1536" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/mab1536_human-mouse-rat-hif-1-alpha-mab-clone-241809-4120241033832.jpg" data-alt="Detection of Human HIF-1 alpha by Immunocytochemistry/Immunofluorescence" title="Detection of Human HIF-1 alpha by Immunocytochemistry/Immunofluorescence" data-caption="Identification of histone-acetylation and HIF-destabilization in H23 cells upon co-treatment with panobinostat and cisplatin. (A) H23 cells were treated with increasing panobinostat concentrations under normoxic conditions and the amount of acetylated histone proteins H3 and H4 was detected by immunoblotting. (B) H23 cells were treated with different combinations of cisplatin or/and panobinostat both under normoxic (NOX) or hypoxic (HOX) conditions. The amounts of Ac-H3 and Ac-H4 were analyzed 24 hours later by immunoblotting. (C) After 24 hours treatment with increasing panobinostat concentrations immunoblotting for HIF-1 alpha and HIF-2 alpha was performed. (D) H23 cells were treated with cisplatin, panobinostat or with combination of both under normoxic and hypoxic conditions. After 24 hours immunoblotting for HIF-1 alpha, class II HDACs (HDAC4, 5, 6 and 7) and HDAC1 (class I) was performed. After stripping beta-actin was used as a loading control. Ac-H3 and H4, acetylated histone proteins; DMSO, vehicle control; P, panobinostat (16 nM); C, cisplatin (16 μM); P + C (16 nM panobinostat + 16 μM cisplatin); NOX, normoxia; HOX, hypoxia. (E) H23 cells were transfected with 10 nM of HIF-1 alpha siRNA and after 24 hours knock-down effects were detected by qRT-PCR and by immunoblotting. (F) H23 cells transfected with HIF-1 alpha siRNA were treated with different cisplatin concentrations for 24 hours under hypoxic conditions and cell viability was measured using the AlamarBlue® assay. Viability of untreated cells was set to 100%. Data were compared to cells transfected with control, non-silencing RNA (CTRL). ns, not significant; * P " data-badges="<span class="biological-validation"></span>" data-application="Immunocytochemistry/ Immunofluorescence" data-header="Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody, Catalog # MAB1536" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/mab1536_human-mouse-rat-hif-1-alpha-mab-clone-241809-41202410325542.jpg" data-alt="Detection of Human HIF-1 alpha by Western Blot" title="Detection of Human HIF-1 alpha by Western Blot" data-caption="XIAP ubiquitinates HIF1 alpha. (A) U2OS cells were transfected with siRNA oligonucleotides and His-tagged ubiquitin as indicated. Lysates were prepared and precipitated using nickel-conjugated beads followed by western blotting using the indicated antibodies. (B) HA-tagged plasmid encoding HIF1 alpha was coexpressed in HEK293 cells with His-tagged ubiquitin (Ub), FLAG-XIAP (X) or FLAG-VHL (V) as indicated. Lysates were prepared 48 h post-transfection and ubiquitinated material was recovered by incubation with nickel-conjugated beads and analysed by western blotting. (C) Biotinylated-HIF1 alpha was coexpressed in HEK293 cells with FLAG-tagged XIAP. HIF1 alpha was recovered from lysates by incubation with streptavidin-coated beads and analysed using antibodies directed against specific ubiquitin chains. (D) U2OS cells were pre-treated with 2μM NSC697923 for 30 min before being exposed to 1% O2 for 3 h. Cells were lysed in the presence of UBQ-TUBEs. Ubiquitin conjugates were purified using NiNTA agarose beads and analysed by immunoblotting using the indicated antibodies. (E) U2OS cells were pre-treated with 2 μM NSC697923 for 30 min before being exposed to 1% O2 for 3 h. Cells were lysed in the presence of TAB2-TUBEs or TAB2-AA-TUBEs as indicated. Proteins conjugated to Lys63-linked ubiquitin chains were purified using Ni-NTA agarose beads and analysed by immunoblot. Image collected and cropped by CiteAb from the following publication (https://academic.oup.com/nar/article/45/16/9336/3896344), licensed under a CC-BY license. Not internally tested by R&D Systems." data-badges="<span class="biological-validation"></span>" data-application="Western Blot" data-header="Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody, Catalog # MAB1536" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/mab1536_human-mouse-rat-hif-1-alpha-mab-clone-241809-41202410325517.jpg" data-alt="Detection of Human HIF-1 alpha by Western Blot" title="Detection of Human HIF-1 alpha by Western Blot" data-caption="Identification of histone-acetylation and HIF-destabilization in H23 cells upon co-treatment with panobinostat and cisplatin. (A) H23 cells were treated with increasing panobinostat concentrations under normoxic conditions and the amount of acetylated histone proteins H3 and H4 was detected by immunoblotting. (B) H23 cells were treated with different combinations of cisplatin or/and panobinostat both under normoxic (NOX) or hypoxic (HOX) conditions. The amounts of Ac-H3 and Ac-H4 were analyzed 24 hours later by immunoblotting. (C) After 24 hours treatment with increasing panobinostat concentrations immunoblotting for HIF-1 alpha and HIF-2 alpha was performed. (D) H23 cells were treated with cisplatin, panobinostat or with combination of both under normoxic and hypoxic conditions. After 24 hours immunoblotting for HIF-1 alpha, class II HDACs (HDAC4, 5, 6 and 7) and HDAC1 (class I) was performed. After stripping beta-actin was used as a loading control. Ac-H3 and H4, acetylated histone proteins; DMSO, vehicle control; P, panobinostat (16 nM); C, cisplatin (16 μM); P + C (16 nM panobinostat + 16 μM cisplatin); NOX, normoxia; HOX, hypoxia. (E) H23 cells were transfected with 10 nM of HIF-1 alpha siRNA and after 24 hours knock-down effects were detected by qRT-PCR and by immunoblotting. (F) H23 cells transfected with HIF-1 alpha siRNA were treated with different cisplatin concentrations for 24 hours under hypoxic conditions and cell viability was measured using the AlamarBlue® assay. Viability of untreated cells was set to 100%. Data were compared to cells transfected with control, non-silencing RNA (CTRL). ns, not significant; * P " data-badges="<span class="biological-validation"></span>" data-application="Western Blot" data-header="Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody, Catalog # MAB1536" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/mab1536_human-mouse-rat-hif-1-alpha-mab-clone-241809-4120241032557.jpg" data-alt="Detection of Human HIF-1 alpha by Western Blot" title="Detection of Human HIF-1 alpha by Western Blot" data-caption="NQO1 enhances HIF-1 alpha expression.(a,b) RKO cells (a) and MDA-MB-231 cells (b) were exposed to 0.5% O2 for 8 h and harvested at the indicated times. Whole-cell lysates were analysed by immunoblotting for HIF-1 alpha, HIF-1 beta, NQO1 and beta-actin. SE, short exposure; LE, long exposure. (c,d) Various concentrations of pshNQO1 and pNQO1-myc-His6 (0–10 μg) were transiently transfected into RKO (c) and MDA-MB-231 (d) cells, respectively. After 48 h, the cells were exposed to 20 or 0.5% O2 for another 2 h, and then harvested. Whole-cell lysates were analysed by immunoblotting for HIF-1 alpha, NQO1 and beta-actin. EV indicates pshCont (c) and pCDNA3.1-myc-His6 (d). SE, short exposure; LE, long exposure. (e) pNQO1-myc-His6 was transiently transfected into RKO/pshNQO1 cells. After 48 h, the cells were exposed to 0.5% O2 for 2 h, and then harvested. Whole-cell lysates were analysed by immunoblotting for HIF-1 alpha, NQO1 and beta-actin. SE, short exposure; LE, long exposure. (f) Mouse embryonic fibroblast wild type and NQO1−/− cells were exposed to 20 or 0.5% O2 for 2 h and then harvested. Whole-cell lysates were analysed by immunoblotting for HIF-1 alpha, NQO1 and beta-actin. SE, short exposure; LE, long exposure. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27966538), licensed under a CC-BY license. Not internally tested by R&D Systems." data-badges="" data-application="Western Blot" data-header="Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody, Catalog # MAB1536" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/mab1536_human-mouse-rat-hif-1-alpha-mab-clone-241809-4120241033843.jpg" data-alt="Detection of Human HIF-1 alpha by Western Blot" title="Detection of Human HIF-1 alpha by Western Blot" data-caption="Ubc13 activity is necessary for HIF1 alpha nuclear retention. (A) ChIP assays using Control IgG and HIF1 alpha antibodies were performed on cell extracts from U2OS cells pre-treated with 2μM NSC697923 for 30 min before being exposed to 1% O2 for 3 h. Immunoprecipitated DNA was analysed by qPCR using the indicated primer pairs and normalised to input genomic DNA. (B and C) U2OS cells were transfected with HA-HIF1 alpha (P402A, P564A) or empty vector and treated with 2 μM NSC697923 for 3 h. Whole cell lysates (B) or nuclear and cytoplasmic extracts (C) were prepared. Extracts were analysed by immunoblotting using the indicated antibodies. (D) Cytoplasmic and nuclear extracts were prepared from U2OS cells treated with 2 μM NSC697923 and 5 ng/ml Leptomycin B as indicated before being exposed to 1% O2 for 3 h. P-values are significant according to the Student's t-test; *P < 0.05, **P < 0.01. Image collected and cropped by CiteAb from the following publication (https://academic.oup.com/nar/article/45/16/9336/3896344), licensed under a CC-BY license. Not internally tested by R&D Systems." data-badges="<span class="biological-validation"></span>" data-application="Western Blot" data-header="Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody, Catalog # MAB1536" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/mab1536_human-mouse-rat-hif-1-alpha-mab-clone-241809-41202410325528.jpg" data-alt="Detection of Human HIF-1 alpha by Western Blot" title="Detection of Human HIF-1 alpha by Western Blot" data-caption="Increasing doses of Allopurinol reduce HIF-1 alpha levels in hypoxic HFFs, without changing HIF mRNA levels but have reduced effect in HUVEC cells.A. Cells were pre-treated with Allopurinol at 10, 100 and 1000 μg/ml for 5, 30 and 60 minutes then were incubated in 1% oxygen for 16 hours. The cells were lysed for assessment of the indicated proteins. HIF-1 alpha levels were quantified using ImageJ software and graph depicts mean and standard deviation of a minimum of three independent experiments. Anova t-test was performed and p values calculated as follows: *p" data-badges="<span class="biological-validation"></span>" data-application="Western Blot" data-header="Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody, Catalog # MAB1536" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/mab1536_human-mouse-rat-hif-1-alpha-mab-clone-241809-41202410333039.jpg" data-alt="Detection of Human HIF-1 alpha by Western Blot" title="Detection of Human HIF-1 alpha by Western Blot" data-caption="NQO1 enhances HIF-1 alpha expression.(a,b) RKO cells (a) and MDA-MB-231 cells (b) were exposed to 0.5% O2 for 8 h and harvested at the indicated times. Whole-cell lysates were analysed by immunoblotting for HIF-1 alpha, HIF-1 beta, NQO1 and beta-actin. SE, short exposure; LE, long exposure. (c,d) Various concentrations of pshNQO1 and pNQO1-myc-His6 (0–10 μg) were transiently transfected into RKO (c) and MDA-MB-231 (d) cells, respectively. After 48 h, the cells were exposed to 20 or 0.5% O2 for another 2 h, and then harvested. Whole-cell lysates were analysed by immunoblotting for HIF-1 alpha, NQO1 and beta-actin. EV indicates pshCont (c) and pCDNA3.1-myc-His6 (d). SE, short exposure; LE, long exposure. (e) pNQO1-myc-His6 was transiently transfected into RKO/pshNQO1 cells. After 48 h, the cells were exposed to 0.5% O2 for 2 h, and then harvested. Whole-cell lysates were analysed by immunoblotting for HIF-1 alpha, NQO1 and beta-actin. SE, short exposure; LE, long exposure. (f) Mouse embryonic fibroblast wild type and NQO1−/− cells were exposed to 20 or 0.5% O2 for 2 h and then harvested. Whole-cell lysates were analysed by immunoblotting for HIF-1 alpha, NQO1 and beta-actin. SE, short exposure; LE, long exposure. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27966538), licensed under a CC-BY license. Not internally tested by R&D Systems." data-badges="" data-application="Western Blot" data-header="Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody, Catalog # MAB1536" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/mab1536_human-mouse-rat-hif-1-alpha-mab-clone-241809-22820241721158.jpg" data-alt="Detection of Human Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody by Immunocytochemistry/ Immunofluorescence" title="Detection of Human Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody by Immunocytochemistry/ Immunofluorescence" data-caption="Generation of 3D tumor spheroid micropillar array.a Photo of 330-micropillar/well chip for effector cell-mediated cytotoxicity. The diameters of micropillar and microwell array spots are 1 and 1.9 mm, respectively. b Generation of 3D tumor spheroid by printing high-density cells (~2500 cells/250 nL) in Matrigel, followed by culturing cancer cells for up to 8 days. c Expression of HIF-1 alpha inside MiaPaCa-2 3D tumor spheroids. Confocal microscopy images of MiaPaCa-2 3D tumor spheroids stained with Hoechst 33342 and labeled with HIF-1 alpha antibody via immunofluorescence. Top views of cross-sectional images in a 3D tumor spheroid at different focal planes (top, middle, and bottom) using Imaris software. A green arrow symbol orients the viewing direction for the cross-sections reconstructed from Z stacks. d 3D view of a 3D tumor spheroid after staining the nucleus and labeling HIF1 alpha. Scale bars show 300 µm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/34290356), licensed under a CC-BY license. Not internally tested by R&D Systems." data-badges="" data-application="Immunocytochemistry/ Immunofluorescence" data-header="Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody, Catalog # MAB1536" data-width="250" data-height="250" class="none"></i> </div> </div> </div> </div> <div class="atc-block-search atc-block-collapsible" data-type="search" data-blockid="ProductCart-MAB1536" > <form action="/commerce/addtocart" method="post" class="px-3 pt-3 add-to-cart-block" data-productcode="MAB1536"> <div class="commerce-atc-full-header atc-header col-four row border-bottom pb-1 m-0"> <div class="commerce-atc-catnum atc-head col-4 col-md-4 font-weight-bold">Catalog #</div> <div class="commerce-atc-availability atc-head col-md-3 font-weight-bold d-none d-md-block">Availability </div> <div class="commerce-atc-price atc-head col-5 col-md-3 font-weight-bold">Size / Price</div> <div class="commerce-atc-quantity atc-head col-3 col-md-2 text-right font-weight-bold">Qty</div> </div> <div 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href="/p/antibodies/human-mouse-rat-hif-1-alpha-hif1a-antibody_af1935" title="Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody"> <h4>Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody </h4> </a> </div> <div class="search_result_brand_logo col-md-3 mb-1 d-flex justify-content-xl-end product-brand-logo"> <img alt="R&D Systems, a Bio-Techne brand" src="https://resources.bio-techne.com/bio-techne-assets/images/logos/bio-techne-rnd-systems.svg" class="d-flex w-75"> </div> </div> <div class="row"> <div class="col-md-9"> <div class="mb-1 d-inline-flex"> <div class="align-middle my-1 mr-3 d-block d-md-inline"> Catalog #: <a href="/p/antibodies/human-mouse-rat-hif-1-alpha-hif1a-antibody_af1935" title="Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody"> AF1935 </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold"> <a href="/p/antibodies/human-mouse-rat-hif-1-alpha-hif1a-antibody_af1935#reviews" class="searchImage_star4"> (1) </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold"> <a href="/p/antibodies/human-mouse-rat-hif-1-alpha-hif1a-antibody_af1935#citations"><span class="icon-icon-product-citations"></span>Citations (68)</a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold text-nowrap"> <a href="/p/antibodies/human-mouse-rat-hif-1-alpha-hif1a-antibody_af1935#product-documents"><span class="icon-icon-pdf text-error-red"></span> Product Documents</a> </div> <div class="d-none d-md-inline my-1"> <a href="#" class="search_result_images"><span class="icon-icon-image-camera-plus"></span> Images (7)</a> </div> </div> <div class="d-none d-md-block"> <span class="search_result_applications"></span><span class="applications_title"><strong>Applications:</strong></span> WB, Simple Western, IP, ChIP, ICC, KO </div> <div class="d-none d-md-block"> <span class="species_title"><strong>Reactivity:</strong></span> Human, Mouse, Rat </div> </div> <div class="col-md-3"> <button class="py-1 btn-default btn-block atc-view-more collapsed" data-toggle="collapse" href="#collapseButton-7" role="button" aria-expanded="false" aria-controls="collapseButton">View More</button> </div> </div> <div class="commerce-atc-search pt-3 collapse" id="collapseButton-7"> <div class="row"> <div class="search_result_description col-xs-12 col-md-9"> <table class="tablesaw tablesaw-stack mb-2"> <tbody> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Reactivity:</strong></td> <td class="pb-1 w-75 text-break"> Human, Mouse, Rat </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Details:</strong></td> <td class="pb-1 w-75 text-break"> Goat IgG Polyclonal </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Applications:</strong></td> <td class="pb-1 w-75 text-break"> WB, Simple Western, IP, ChIP, ICC, +1 More </td> </tr> </tbody> </table> <div class="compare_tool_select form-inline mb-2" data-sku="af1935"></div> </div> <div class="search_result_image_wrapper col-xs-12 col-md-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="rnd"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src=https://resources.bio-techne.com/images/products/antibody/HIF-1_alpha_AF1935_Western_Blot_5158.jpg data-srcset=https://resources.bio-techne.com/images/products/antibody/HIF-1_alpha_AF1935_Western_Blot_5158.jpg alt="Detection of Human HIF-1a/HIF1A antibody by Western Blot." title="Detection of Human HIF‑1 alpha/HIF1A by Western Blot." data-caption="Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 150 µM CoCl<sub>2</sub>for 16 hours. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat HIF-1a/HIF1A Antigen-affinity Purified Polyclonal Antibody, followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for HIF-1a/HIF1A at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." data-badges="<span class="biological-validation"></span>" data-application="Western Blot" data-header="Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody, Catalog # AF1935" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> <span class="image-count-text align-middle">(7)</span> </div> </div> </div> <i data-src="https://resources.bio-techne.com/images/products/antibody/HIF-1_alpha_AF1935_Chromatin_Immunoprecipitation_11221.jpg" data-alt="Detection of HIF-1a/HIF1A-regulated Genes antibody by Chromatin Immunoprecipitation." title="Detection of HIF‑1 alpha/HIF1A-regulated Genes by Chromatin Immunoprecipitation." data-caption="Mouse primary kidney cells treated with 150 µM CoCl2 for overnight were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. HIF-1a/DNA complexes were immunoprecipitated using 5 µg Goat Anti-Human/Mouse/Rat HIF-1a/HIF1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1935) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The epo promoter was detected by standard PCR." data-badges="<span class="biological-validation"></span>" data-application="Chromatin Immunoprecipitation (ChIP)" data-header="Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody, Catalog # AF1935" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/antibody/HIF1_alpha_AF1935_Knockout_Validated_22389.jpg" data-alt="HIF-1a/HIF1A Antibody Specificity is Shown by Immunocytochemistry in Knockout Cell Line by Immunocytochemistry (ICC)." title="HIF‑1 alpha/HIF1A Specificity is Shown by Immunocytochemistry in Knockout Cell Line." data-caption="HIF-1a/HIF1A was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line treated with DFO but is not detected in HIF-1a/HIF1A knockout (KO) HeLa Human Cell Line cell line using Goat Anti-Human/Mouse/Rat HIF-1a/HIF1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1935) at 0.3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." data-badges="<span class="biological-validation"></span>" data-application="Immunocytochemistry" data-header="Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody, Catalog # AF1935" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/antibody/HIF1_alpha_AF1935_Simple_Western_16623.jpg" data-alt="Detection of Human HIF-1a/HIF1A antibody by Simple WesternTM." title="Detection of Human HIF‑1 alpha/HIF1A by Simple Western<sup>TM</sup>." data-caption="Simple Western lane view shows lysates of A549 human lung carcinoma cell line untreated (-) or treated (+) with Hypoxia (1% O<sub>2</sub>), loaded at 0.2 mg/mL. A specific band was detected for HIF-1a/HIF1A at approximately 115 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat HIF-1a/HIF1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1935) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." data-badges="<span class="biological-validation"></span>" data-application="Simple Western" data-header="Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody, Catalog # AF1935" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/af1935_human-mouse-rat-hif-1-alpha-affinity-purified-polyclonal-ab-4120241048432.jpg" data-alt="Detection of Mouse HIF-1 alpha by Simple Western" title="Detection of Mouse HIF-1 alpha by Simple Western" data-caption="Ten days of IH increases hippocampal HIF1a and disrupts Barnes maze performance in wild-type mice but not in HIF1a+/−. A, left, Representative digitized Western blotting images for HIF1a (103 kDa) and PCNA (40 kDa) in hippocampal nuclear protein fractions from control (n = 4) and IH10 (n = 4). Right, Quantification of HIF1a protein normalized to PCNA revealed that nuclear HIF1a was increased in IH10 when compared with control (p = 0.019). B, Total latency to exit the Barnes maze during three training sessions in control (n = 10) and in IH10 (n = 11). Each blue (control) and red (IH10) line represents an individual performance during training. Training to the exit was conducted over three sessions. Each session was separated by 24 hours. C, Left, During the probe trial, the distance traveled to initially enter the exit zone was shorter in control when compared with IH10 (p = 0.048). Right, Latency to initial entry was smaller in control as well (p = 0.034). D, Heat maps of the mean entry probability across all false exits (1–19) and the exit zone during probe trial for the control and IH10. Comparison of entry probability into the exit zone during the probe trial reveals that control has a greater probability for entering the exit zone when compared with IH10 (p = 0.004). E, Left, Representative digitized Western blotting images HIF1a and PCNA in hippocampal nuclear protein fractions from 0-HIF1a+/− (n = 4) and 10-HIF1a+/− (n = 4). Right, Quantification of HIF1a protein normalized to PCNA revealed that nuclear HIF1a is similar between 0-HIF1a+/− and 10-HIF1a+/− (p = 0.84). F, Total latency to exit the Barnes maze during three training sessions in 0-HIF1a+/− (n = 7) and in 10-HIF1a+/− (n = 8). Each gray (0-HIF1a+/−) and yellow (10-HIF1a+/−) line represents an individual performance during training. All experimental groups exhibit decreased total latency over the course of training. G, Left, In HIF1a+/−, the distance initial to initial entry into the exit zone was similar between 0-HIF1a+/− and 10-HIF1a+/− (p = 0.55). Right, Latency to initial entry into the exit zone during the probe trial were similar between 0-HIF1a+/− and 10-HIF1a+/− (p = 0.39). H, Heat maps of the mean entry probability into all zones during the probe trial for 0-HIF1a+/− and 10-HIF1a+/−. Entry probability was similar between 0-HIF1a+/− and 10-HIF1a+/− (p = 0.21); *p " data-badges="" data-application="Simple Western" data-header="Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody, Catalog # AF1935" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/af1935_human-mouse-rat-hif-1-alpha-affinity-purified-polyclonal-ab-41202410485442.jpg" data-alt="Detection of Human HIF-1 alpha by Western Blot" title="Detection of Human HIF-1 alpha by Western Blot" data-caption="The DU145FP cells express higher levels of cancer stemness related genes. (A) Transcriptome analyses of prostate cancer stemness-related markers. The Log2 transformed ratio of FPKM values (e.g., FP/WT) are indicated by color-coded index bars. (B) Cells were stained with fluorophore conjugated monoclonal antibodies against CD44 and EPCAM, the antibody against integrin alpha2 beta3 without fluorophore conjugation was detected by using secondary antibody conjugated with fluorophore, and stained cells were analyzed by flow cytometry. The scatter plots and histograms show representative results. CTRL denote cell stained with isotype control antibody or only secondary antibody; ST denote cell stained with antibody against EPCAM and integrin alpha2 beta3; US denote unstained cells. (C) Immunoblotting confirmation of the protein expression of A-tubulin, GAPDH, SOX9, SLUG, HIF1A, HIF2A, CD44 and integrin alpha2 beta3. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28698547), licensed under a CC-BY license. Not internally tested by R&D Systems." data-badges="" data-application="Western Blot" data-header="Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody, Catalog # AF1935" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/af1935_human-mouse-rat-hif-1-alpha-affinity-purified-polyclonal-ab-41202410485445.jpg" data-alt="Detection of Mouse HIF-1 alpha by Simple Western" title="Detection of Mouse HIF-1 alpha by Simple Western" data-caption="Ten days of IH increases hippocampal HIF1a and disrupts Barnes maze performance in wild-type mice but not in HIF1a+/−. A, left, Representative digitized Western blotting images for HIF1a (103 kDa) and PCNA (40 kDa) in hippocampal nuclear protein fractions from control (n = 4) and IH10 (n = 4). Right, Quantification of HIF1a protein normalized to PCNA revealed that nuclear HIF1a was increased in IH10 when compared with control (p = 0.019). B, Total latency to exit the Barnes maze during three training sessions in control (n = 10) and in IH10 (n = 11). Each blue (control) and red (IH10) line represents an individual performance during training. Training to the exit was conducted over three sessions. Each session was separated by 24 hours. C, Left, During the probe trial, the distance traveled to initially enter the exit zone was shorter in control when compared with IH10 (p = 0.048). Right, Latency to initial entry was smaller in control as well (p = 0.034). D, Heat maps of the mean entry probability across all false exits (1–19) and the exit zone during probe trial for the control and IH10. Comparison of entry probability into the exit zone during the probe trial reveals that control has a greater probability for entering the exit zone when compared with IH10 (p = 0.004). E, Left, Representative digitized Western blotting images HIF1a and PCNA in hippocampal nuclear protein fractions from 0-HIF1a+/− (n = 4) and 10-HIF1a+/− (n = 4). Right, Quantification of HIF1a protein normalized to PCNA revealed that nuclear HIF1a is similar between 0-HIF1a+/− and 10-HIF1a+/− (p = 0.84). F, Total latency to exit the Barnes maze during three training sessions in 0-HIF1a+/− (n = 7) and in 10-HIF1a+/− (n = 8). Each gray (0-HIF1a+/−) and yellow (10-HIF1a+/−) line represents an individual performance during training. All experimental groups exhibit decreased total latency over the course of training. G, Left, In HIF1a+/−, the distance initial to initial entry into the exit zone was similar between 0-HIF1a+/− and 10-HIF1a+/− (p = 0.55). Right, Latency to initial entry into the exit zone during the probe trial were similar between 0-HIF1a+/− and 10-HIF1a+/− (p = 0.39). H, Heat maps of the mean entry probability into all zones during the probe trial for 0-HIF1a+/− and 10-HIF1a+/−. Entry probability was similar between 0-HIF1a+/− and 10-HIF1a+/− (p = 0.21); *p " data-badges="" data-application="Simple Western" data-header="Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody, Catalog # AF1935" data-width="250" data-height="250" class="none"></i> </div> </div> </div> </div> <div class="atc-block-search atc-block-collapsible" data-type="search" data-blockid="ProductCart-AF1935" > <form action="/commerce/addtocart" method="post" class="px-3 pt-3 add-to-cart-block" data-productcode="AF1935"> <div class="commerce-atc-full-header atc-header col-four row border-bottom pb-1 m-0"> <div class="commerce-atc-catnum atc-head col-4 col-md-4 font-weight-bold">Catalog #</div> <div class="commerce-atc-availability atc-head col-md-3 font-weight-bold d-none d-md-block">Availability </div> <div class="commerce-atc-price atc-head col-5 col-md-3 font-weight-bold">Size / Price</div> <div class="commerce-atc-quantity atc-head col-3 col-md-2 text-right font-weight-bold">Qty</div> </div> <div class="spinner-border spinner-border-sm mt-3 ml-3" role="status"> <span class="sr-only">Loading...</span> </div> <div class="d-none search-product-mask"> <div id="ProductCart-AF1935" class="atc-lines"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="AF1935"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> AF1935 </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="AF1935-SP"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> AF1935-SP </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> </div> <div class="atc-control ProductCart-AF1935-control text-right"> <div class="row d-flex justify-content-end mx-0 p-0"> <div class="order-md-last m___button-atc mx-1"> <button type="submit" value="Add To Cart" class="atc-add-button btn a___button-atc m-1 col-xs-4 order-last btn-md-block w-100"> Add To Cart </button> </div> <div class="m___button-atc mx-1"> <a href="/services/bulk-quotes?product_code=AF1935" class="atc-bulk-button btn a___button-secondary m-1 col-xs-4 btn-md-block w-100" rel="nofollow">Bulk Order</a> </div> </div> </div> </div> </form> </div> </div> </div> </div> <div class="search_result atc-container pb-3" data-brand="novus" data-category="Primary Antibodies" data-id="NB100-123" data-name="HIF-1 alpha Antibody (H1alpha67)" data-position="8"> <div class="search_details border bg-white p-3 rounded"> <div class="row"> <div class="col-md-9"> <a href="/p/antibodies/hif-1-alpha-antibody-h1alpha67_nb100-123" title="HIF-1 alpha Antibody (H1alpha67)"> <h4>HIF-1 alpha Antibody (H1alpha67) </h4> </a> </div> <div class="search_result_brand_logo col-md-3 mb-1 d-flex justify-content-xl-end product-brand-logo"> <img alt="Novus Biologicals, a Bio-Techne brand" src="https://resources.bio-techne.com/bio-techne-assets/images/logos/bio-techne-novus-biologicals.svg" class="d-flex w-75"> </div> </div> <div class="row"> <div class="col-md-9"> <div class="mb-1 d-inline-flex"> <div class="align-middle my-1 mr-3 d-block d-md-inline"> Catalog #: <a href="/p/antibodies/hif-1-alpha-antibody-h1alpha67_nb100-123" title="HIF-1 alpha Antibody (H1alpha67)"> NB100-123 </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold"> <a href="/p/antibodies/hif-1-alpha-antibody-h1alpha67_nb100-123#reviews" class="searchImage_star4"> (10) </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold"> <a href="/p/antibodies/hif-1-alpha-antibody-h1alpha67_nb100-123#citations"><span class="icon-icon-product-citations"></span>Citations (160)</a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold text-nowrap"> <a href="/p/antibodies/hif-1-alpha-antibody-h1alpha67_nb100-123#product-documents"><span class="icon-icon-pdf text-error-red"></span> Product Documents</a> </div> <div class="d-none d-md-inline my-1"> <a href="#" class="search_result_images"><span class="icon-icon-image-camera-plus"></span> Images (38)</a> </div> </div> <div class="d-none d-md-block"> <span class="search_result_applications"></span><span class="applications_title"><strong>Applications:</strong></span> IHC, WB, ELISA, ICC/IF, Flow, ChIP, GS, IP, KD, KO, Simple Western </div> <div class="d-none d-md-block"> <span class="species_title"><strong>Reactivity:</strong></span> Human, Mouse, Rat, Canine, Bovine, +7 More </div> </div> <div class="col-md-3"> <button class="py-1 btn-default btn-block atc-view-more collapsed" data-toggle="collapse" href="#collapseButton-8" role="button" aria-expanded="false" aria-controls="collapseButton">View More</button> </div> </div> <div class="commerce-atc-search pt-3 collapse" id="collapseButton-8"> <div class="row"> <div class="search_result_description col-xs-12 col-md-9"> <table class="tablesaw tablesaw-stack mb-2"> <tbody> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Reactivity:</strong></td> <td class="pb-1 w-75 text-break"> Human, Mouse, Rat, Canine, Bovine, +7 More </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Details:</strong></td> <td class="pb-1 w-75 text-break"> Mouse IgG2B Monoclonal Clone #H1alpha67 </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Applications:</strong></td> <td class="pb-1 w-75 text-break"> IHC, WB, ELISA, ICC/IF, Flow, +6 More </td> </tr> </tbody> </table> <div class="compare_tool_select form-inline mb-2" data-sku="nb100-123"></div> </div> <div class="search_result_image_wrapper col-xs-12 col-md-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="novus"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src=https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-H1alpha67-Immunohistochemistry-NB100-123-img0033.jpg data-srcset=https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-H1alpha67-Immunohistochemistry-NB100-123-img0033.jpg alt="Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-123]" title="Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-123]" data-caption="Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - Results of in situ hybridization and immunohistochemistry on thin adjacent section to detect expression of HIF-1a1.2 mRNA and HIF1a protein in malignant and benign prostate tissue. In situ hybridization (antisense probe, Fig. 3a) and immunostaining with HIF1a Ab2 (Fig. 3c) on thin adjacent sections of NE-differentiated prostate adenocarcinoma showed co-localization of HIF1a1.2 transcript and HIF-1a protein. Incubation with sense probe did not generate any detectable hybridization signals (Fig. 3b). Both In situ hybridization (Fig. 3d antisense) and HIF-1a Ab2 immunostaining (Fig. 3f) were negative in non-NE-differentiated prostate adenocarcinoma. In situ hybridization with sense probe performed on non-NE-differentiated prostate cancer (Fig. 3e). Image collected and cropped by CiteAb from the following publication (https://www.biomedcentral.com/1471-2407/10/385), licensed under a CC-BY license." data-badges="<span class="orthogonal-validation"></span>" data-application="Immunohistochemistry" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-123" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> <span class="image-count-text align-middle">(38)</span> </div> </div> </div> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-H1alpha67-Knockdown-Validated-NB100-123-img0028.jpg" data-alt="Knockdown Validated: HIF-1 alpha Antibody (H1alpha67) [NB100-123]" title="Knockdown Validated: HIF-1 alpha Antibody (H1alpha67) [NB100-123]" data-caption="Knockdown Validated: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - HIF-1 inhibits the apoptosis of hypoxic glioblastoma cells. U87MG cells were transfected with siRNA against HIF-1alpha. Forty-eight hours after transfection, cells were incubated for 2 h in hypoxia (1% O2). HIF-1alpha was detected by immunoblotting. beta-Actin was used as a loading control. The results are representative for three independent experiments. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/cddis2013562) licensed under a CC-BY license." data-badges="<span class="knockout-validation"></span>" data-application="Knockdown Validated" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-123" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-H1alpha67-Western-Blot-NB100-123-img0030.jpg" data-alt="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-123]" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-123]" data-caption="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - MLN4924 induces accumulation of HIF1a in a time dependent manner. Cells were treated with 0.1uM MLN4924 for indicated time periods, followed by IB with indicated antibodies. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/cddis2012125) licensed under a CC-BY license." data-badges="<span class="biological-validation"></span>" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-123" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-H1alpha67-Immunohistochemistry-NB100-123-img0006.jpg" data-alt="Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-123]" title="Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-123]" data-caption="Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - Analysis of HIF-1 alpha in human lung tissue. Image courtesy of product review by Aneta Gandjeva." data-badges="" data-application="Immunohistochemistry" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-123" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-H1alpha67-Immunoprecipitation-NB100-123-img0027.jpg" data-alt="Immunoprecipitation: HIF-1 alpha Antibody (H1alpha67) [NB100-123]" title="Immunoprecipitation: HIF-1 alpha Antibody (H1alpha67) [NB100-123]" data-caption="Immunoprecipitation: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - MG132 attenuates the inhibitory effect of interleukin-1beta on HIF-1/AM axis. U87MG cells were incubated for 2 h in hypoxia (1% O2) with or without interleukin-1beta (10 ng/ml). The proteasome inhibitor MG132 was added 30 min in advance to prevent ubiquitinated HIF-1alpha from proteasomal degradation. HIF-1alpha was precipitated by anti-HIF-1alpha antibody. Ubiquitin was detected by immunoblotting. Non-specific mouse IgG was used as a negative control. The results are representative for three independent experiments Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/cddis2013562) licensed under a CC-BY license." data-badges="" data-application="Immunoprecipitation" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-123" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-H1alpha67-Western-Blot-NB100-123-img0023.jpg" data-alt="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-123]" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-123]" data-caption="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - Analysis using the HRP conjugate of NB100-123. Detection of HIF-1 alpha in cobalt chloride treated/untreated COS-7 nuclear extracts using NB100-123." data-badges="<span class="biological-validation"></span>" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-123" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-H1alpha67-Western-Blot-NB100-123-img0024.jpg" data-alt="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-123]" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-123]" data-caption="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - Analysis using the HRP conjugate of NB100-123. On day 1, MEF cells (+/+,-/-), were grown on 15cm dish (2x10 to the 6th cells). On day 2, cells were exposed to hypoxia for 4hrs. Cells were washed with ice cold PBS twice and whole cell protein was extracted extracted with RIPA buffer fortified with protease. Upon quantification, 100ug of protein was fractionated on 7% polyacralymide gel. Gel was transferred overnight onto nitrocellulose membrane. The membrane was probed with HIF-1 alpha monoclonal antibody at a 1:500 dilution (NB100-123). The secondary antibody was conjugated with HRP and was used at a 1:2500 dilution. Photo courtesy of Dr. Gregg Semenza, Johns Hopkins University." data-badges="<span class="biological-validation"></span>" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-123" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-H1alpha67-Immunocytochemistry-Immunofluorescence-NB100-123-img0035.jpg" data-alt="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody (H1alpha67) [NB100-123]" title="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody (H1alpha67) [NB100-123]" data-caption="Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - Dapagliflozin protects against metabolic switch by inhibition of HIF-1 alpha. Representative images of PTCs incubated with 10 umol/l of molidustat for 6 h with immunofluorescent staining for HIF-1 alpha. Scale bar = 10 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33187129/) licensed under a CC-BY license." data-badges="<span class="biological-validation"></span>" data-application="Immunocytochemistry/ Immunofluorescence" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-123" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-H1alpha67-Immunohistochemistry-Paraffin-NB100-123-img0034.jpg" data-alt="Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (H1alpha67) [NB100-123]" title="Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (H1alpha67) [NB100-123]" data-caption="Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - Immunohistochemical detection of HIF-1alpha protein in human tissues. The nature of the tissue is indicated on top of each figure. Original magnifications are as follows: A, x400; B, x400; C, x100; D, x100; E, x100; F, x100; G, x40; H, x40. Image collected and cropped by CiteAb from the following publication (https://bmcgenet.biomedcentral.com/articles/10.1186/1471-2156-5-27), licensed under a CC-BY license." data-badges="" data-application="Immunohistochemistry-Paraffin" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-123" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-H1alpha67-Immunocytochemistry-Immunofluorescence-NB100-123-img0011.jpg" data-alt="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody (H1alpha67) [NB100-123]" title="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody (H1alpha67) [NB100-123]" data-caption="Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - Staining in pig endothelial cells under hypoxia condition using NB100-123. Image from verified customer review." data-badges="" data-application="Immunocytochemistry/ Immunofluorescence" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-123" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-H1alpha67-Immunohistochemistry-Paraffin-NB100-123-img0012.jpg" data-alt="Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (H1alpha67) [NB100-123]" title="Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (H1alpha67) [NB100-123]" data-caption="Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - Staining on pig tissue (brown) using NB100-123. Image from verified customer review." data-badges="" data-application="Immunohistochemistry-Paraffin" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-123" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-H1alpha67-Immunohistochemistry-NB100-123-img0026.jpg" data-alt="Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-123]" title="Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-123]" data-caption="Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - Staining of biotin conjugated HIF-1 alpha (NB100-123B) in human kidney." data-badges="" data-application="Immunohistochemistry" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-123" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-H1alpha67-Immunoprecipitation-NB100-123-img0025.jpg" data-alt="Immunoprecipitation: HIF-1 alpha Antibody (H1alpha67) [NB100-123]" title="Immunoprecipitation: HIF-1 alpha Antibody (H1alpha67) [NB100-123]" data-caption="Immunoprecipitation: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - Immunoprecipitation of HIF-1alpha using NB100-123 in COS7 CoCl2 treated lysate. Heavy and light chains are also detected. Image using the HRP form of this antibody." data-badges="<span class="biological-validation"></span>" data-application="Immunoprecipitation" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-123" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-123_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-271220231253343.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-123] -" data-caption="Immunohistological and western blot analyses of HIF-1 alpha.(A) PAB liver stained by HIF-1 alpha (original magnification, ×20). (B) Sham-operated control liver stained by HIF-1 alpha (original magnification, ×20). (C) Enlarged view of the boxed area in the Image (A) (original magnification, ×100). (D) Western blot analysis of HIF-1 alpha (mean ± SEM), * P" data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-123" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/antibody/nb100-123_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-immunocytochemistry-immunofluorescence-317202411274.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Immunocytochemistry/Immunofluorescence: Mouse Monoclonal HIF-1 alpha Antibody (H1alpha67) [NB100-123]" data-caption="Immunocytochemistry/Immunofluorescence: Mouse Monoclonal HIF-1 alpha Antibody (H1alpha67) [NB100-123] - Images of HIF-1 alpha localization (red) in CTR and CoCl2-treated oAECs after 24 h of culture. Image from a verified customer review." data-badges="<span class="biological-validation"></span>" data-application="Immunocytochemistry/ Immunofluorescence" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-123" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/antibody/nb100-123_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-western-blot-3172024112840..jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: Mouse Monoclonal HIF-1 alpha Antibody (H1alpha67) [NB100-123]" data-caption="Western Blot: Mouse Monoclonal HIF-1 alpha Antibody (H1alpha67) [NB100-123] - Image of HIF-1 alpha activation in AECs control cells (CTR) and after 24h of 10 and 50µM of CoCl2-treatment; HIF-1 alpha time course at shorter CoCl2 incubation times. Image from a verified customer review." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-123" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/antibody/nb100-123_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-western-blot-3172024112948..jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: Mouse Monoclonal HIF-1 alpha Antibody (H1alpha67) [NB100-123]" data-caption="Western Blot: Mouse Monoclonal HIF-1 alpha Antibody (H1alpha67) [NB100-123] - Image of HIF-1 alpha activation in AECs control cells (CTR) and after 24h of 10 and 50µM of CoCl2-treatment; HIF-1 alpha time course at shorter CoCl2 incubation times. Image from a verified customer review." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-123" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-123_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-31020241539595.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-123] -" data-caption="Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - Immunohistochemical detection of HIF-1 alpha protein in human tissues. The nature of the tissue is indicated on top of each figure. Original magnifications are as follows: A, ×400; B, ×400; C, ×100; D, ×100; E, ×100; F, ×100; G, ×40; H, ×40. Image collected & cropped by CiteAb from the following publication (http://bmcgenet.biomedcentral.com/articles/10.1186/1471-2156-5-27), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Immunohistochemistry" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-123" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-123_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-31020241517525.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-123] -" data-caption="Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - Immunohistochemical detection of HIF-1 alpha protein in human tissues. The nature of the tissue is indicated on top of each figure. Original magnifications are as follows: A, ×400; B, ×400; C, ×100; D, ×100; E, ×100; F, ×100; G, ×40; H, ×40. Image collected & cropped by CiteAb from the following publication (http://bmcgenet.biomedcentral.com/articles/10.1186/1471-2156-5-27), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Immunohistochemistry" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-123" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-123_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-31020241535692.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-123] -" data-caption="Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - Immunohistochemical detection of HIF-1 alpha protein in human tissues. The nature of the tissue is indicated on top of each figure. Original magnifications are as follows: A, ×400; B, ×400; C, ×100; D, ×100; E, ×100; F, ×100; G, ×40; H, ×40. Image collected & cropped by CiteAb from the following publication (http://bmcgenet.biomedcentral.com/articles/10.1186/1471-2156-5-27), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Immunohistochemistry" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-123" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-123_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-310202415371912.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-123] -" data-caption="Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - Immunohistochemical detection of HIF-1 alpha protein in human tissues. The nature of the tissue is indicated on top of each figure. Original magnifications are as follows: A, ×400; B, ×400; C, ×100; D, ×100; E, ×100; F, ×100; G, ×40; H, ×40. Image collected & cropped by CiteAb from the following publication (http://bmcgenet.biomedcentral.com/articles/10.1186/1471-2156-5-27), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Immunohistochemistry" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-123" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-123_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-310202415345347.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-123] -" data-caption="Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - Immunohistochemical detection of HIF-1 alpha protein in human tissues. The nature of the tissue is indicated on top of each figure. Original magnifications are as follows: A, ×400; B, ×400; C, ×100; D, ×100; E, ×100; F, ×100; G, ×40; H, ×40. Image collected & cropped by CiteAb from the following publication (http://bmcgenet.biomedcentral.com/articles/10.1186/1471-2156-5-27), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Immunohistochemistry" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-123" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-123_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-310202415175274.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-123] -" data-caption="Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - Immunohistochemical detection of HIF-1 alpha protein in human tissues. The nature of the tissue is indicated on top of each figure. Original magnifications are as follows: A, ×400; B, ×400; C, ×100; D, ×100; E, ×100; F, ×100; G, ×40; H, ×40. Image collected & cropped by CiteAb from the following publication (http://bmcgenet.biomedcentral.com/articles/10.1186/1471-2156-5-27), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Immunohistochemistry" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-123" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-123_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-310202415371949.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-123] -" data-caption="Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - Immunohistochemical detection of HIF-1 alpha protein in human tissues. The nature of the tissue is indicated on top of each figure. Original magnifications are as follows: A, ×400; B, ×400; C, ×100; D, ×100; E, ×100; F, ×100; G, ×40; H, ×40. Image collected & cropped by CiteAb from the following publication (http://bmcgenet.biomedcentral.com/articles/10.1186/1471-2156-5-27), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Immunohistochemistry" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-123" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-123_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-31020241535630.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-123] -" data-caption="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - Immunohistological & western blot analyses of HIF-1 alpha.(A) PAB liver stained by HIF-1 alpha (original magnification, ×20). (B) Sham-operated control liver stained by HIF-1 alpha (original magnification, ×20). (C) Enlarged view of the boxed area in the Image (A) (original magnification, ×100). (D) Western blot analysis of HIF-1 alpha (mean ± SEM), * P" data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-123" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-123_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-310202415175237.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody (H1alpha67) [NB100-123] -" data-caption="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - Analysis of CBF treatment in HCT116 implanted mouse models & tumour tissue samples. (a) Tumour size versus CBF treatment. The lowest tumour growth rate was found in i.p. group during CBF treatment. (b) Analysis of HIF-1 alpha mRNA level in tumour tissues. There was a significant upregulated HIF-1 alpha level in i.p. group. Results are means with standard errors from 4 replicates. (c) Inhibition of HIF-1 alpha nuclear translocation in the i.t. sample. CBF strongly inhibited HIF-1 alpha nuclear accumulation in the tumour of xenografts implanted with HCT116 cells. Scale bars equal 10 μm. White arrows: HIF-1 alpha in the cytosol. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/23818933), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Immunocytochemistry/ Immunofluorescence" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-123" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-123_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-31020241535686.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-123] -" data-caption="Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - Immunohistochemical detection of HIF-1 alpha protein in human tissues. The nature of the tissue is indicated on top of each figure. Original magnifications are as follows: A, ×400; B, ×400; C, ×100; D, ×100; E, ×100; F, ×100; G, ×40; H, ×40. Image collected & cropped by CiteAb from the following publication (http://bmcgenet.biomedcentral.com/articles/10.1186/1471-2156-5-27), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Immunohistochemistry" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-123" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-123_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-310202415384170.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-123] -" data-caption="Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - CycT alleviates tumor hypoxia. The effects of CycT on the levels of exogenous hypoxia-marker pimonidazole labeling (A), the levels of hypoxia-inducible CA9 enzyme (B), & the levels of hypoxia-inducible factor HIF1 alpha (C) in orthotopic tumor xenografts. Scale bar: Montage, 1 mm; 10X, 20 μm. Data are plotted as mean ± SEM. For statistical analysis, the levels in treated tumors were compared to the levels in control tumors with a Welch 2-sample t-test. **p-value " data-badges="" data-application="Immunohistochemistry" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-123" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-123_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-310202415291911.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-123] -" data-caption="Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - Immunohistochemical detection of HIF-1 alpha protein in human tissues. The nature of the tissue is indicated on top of each figure. Original magnifications are as follows: A, ×400; B, ×400; C, ×100; D, ×100; E, ×100; F, ×100; G, ×40; H, ×40. Image collected & cropped by CiteAb from the following publication (http://bmcgenet.biomedcentral.com/articles/10.1186/1471-2156-5-27), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Immunohistochemistry" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-123" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-123_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-3102024165724.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-123] -" data-caption="Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - Schematic demonstration of the three HIF1 alpha antibodies used in this study & their epitopes (Figs. 2a & b). Ab1 is a polyclonal antibody raised against N-domain of wild type HIF1 alpha & does not recognize HIF1 alpha1.2 due to it's different N-terminal part (Fig. 2a). Ab2 & Ab3 are monoclonal & polyclonal antibodies, respectively, with epitopes in the common parts of HIF1 alpha & HIF1 alpha1.2 (Fig.2a & b). Immunohistochemical analysis performed on thin adjacent sections of NE-differentiated prostate cancer using the HIF1 alpha antibodies Ab1 (Fig. 2c), Ab 2 (Fig. 2d), Ab 3 (Fig. 2e) & HIF1 beta antibody (Fig. 2f). Immunopositivity was detected for HIF1 alpha with Ab2 & Ab3 while HIF1 alpha Ab1 produced no detectable staining. HIF1 beta was also positive in adjacent section (Fig. 2f). Double staining of chromogranin A & androgen receptor antigens on adjacent sections (Fig. 2g) showed immunopositivity for chromogranin A (Fast red). Androgen receptor antibody (DAB, brown) produced no staining. Immunostaining of benign prostate tissue with HIF1 alpha Ab3 showed immunopositivity in NE-like cells of benign prostate tissue (Fig. 2h). In addition, HIF1 beta antibody recognized benign NE-like cells in benign prostate hyperplasia (Fig. 2i). Double staining with HIF1 alpha Ab3 & HIF1 beta (Fig. 2j) shows co-localization of the two proteins in NE-like cells of benign prostate hyperplasia (HIF1 alpha Ab3 red stain, HIF1 beta brown stain). Panels a, b, c, d, e, f & g: 40× objective. Panels h, i & j: 60× objective. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/20663134), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Immunohistochemistry" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-123" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-123_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-31020241682120.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody (H1alpha67) [NB100-123] -" data-caption="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - GAD65/67-positive neurons expressed HIF-1 alpha under hypoxic conditions. A) HIF-1 alpha expression co-localized with GAD65/67-ir in neurons exposed to hypoxia when compared to normoxia (upper panel) or GAD65/67-negative neurons in hypoxia (bottom panel, open arrow). B) Quantification shows the percentage of HIF-1 alpha-expressing GAD65/67-positive neurons after hypoxia in vitro (mean ± SD; from n = 6 cultures). C)In vivo immunostaining illustrates HIF-1 alpha-positive (bottom panel, solid arrow) & HIF-1 alpha-negative (bottom panel, open arrow) in GAD65/67-ir neurons in the ipsilateral region, whereas the contralateral region shows no HIF-1 alpha staining in GAD65/67-ir neurons (see Figure 1 for region selection). Scale bars, 10 μm (A); 20 μm (B). Image collected & cropped by CiteAb from the following publication (https://actaneurocomms.biomedcentral.com/articles/10.1186/2051-5960-2-51), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Immunocytochemistry/ Immunofluorescence" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-123" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-123_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-310202415533818.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody (H1alpha67) [NB100-123] -" data-caption="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - GAD65/67-positive neurons expressed HIF-1 alpha under hypoxic conditions. A) HIF-1 alpha expression co-localized with GAD65/67-ir in neurons exposed to hypoxia when compared to normoxia (upper panel) or GAD65/67-negative neurons in hypoxia (bottom panel, open arrow). B) Quantification shows the percentage of HIF-1 alpha-expressing GAD65/67-positive neurons after hypoxia in vitro (mean ± SD; from n = 6 cultures). C)In vivo immunostaining illustrates HIF-1 alpha-positive (bottom panel, solid arrow) & HIF-1 alpha-negative (bottom panel, open arrow) in GAD65/67-ir neurons in the ipsilateral region, whereas the contralateral region shows no HIF-1 alpha staining in GAD65/67-ir neurons (see Figure 1 for region selection). Scale bars, 10 μm (A); 20 μm (B). Image collected & cropped by CiteAb from the following publication (https://actaneurocomms.biomedcentral.com/articles/10.1186/2051-5960-2-51), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Immunocytochemistry/ Immunofluorescence" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-123" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-123_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-31020241682131.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody (H1alpha67) [NB100-123] -" data-caption="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - Levels of GSH in cortical neurons exposed to hypoxia. A) During hypoxia, GSH increased in MAP2-ir neurons with round somata (bottom panel, open arrow) & decreased in neurons with pyramidal-like morphology (upper panel, solid arrow). B) GSH increased in a subset of GAD65/67–ir neurons exposed to hypoxia (bottom panel, open arrow). C) Elevated HIF-1 alpha expression in GAD65/67–ir neurons containing high levels of GSH when exposed to hypoxia (middle panel). BSO treatment decreased HIF-1 alpha expression in GAD65/67-positive neurons during hypoxia. D) Percentages of HIF-1 alpha-expressing GAD65/67-ir neurons expressing a high level of GSH & GAD65/67-ir neurons expressing a low levels of GSH & HIF-1 alpha (mean ± SD; n = 3). The “low” level referred to the level of GSH in normoxic GAD65/67 neurons that was normalized to 1. The “high” level referred to the elevated GSH level in GAD65/67 neurons with a mean of 1.40 ± 0.10. E) Total MCB-GSH intensity in GAD65/67-positive neurons with & without BSO after hypoxia (mean ± SD; 8-10 neurons quantified from each experiment, n = 3 independent cultures). Scale bars, 20 μm. Image collected & cropped by CiteAb from the following publication (https://actaneurocomms.biomedcentral.com/articles/10.1186/2051-5960-2-51), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Immunocytochemistry/ Immunofluorescence" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-123" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-123_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-310202415525260.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-123] -" data-caption="Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - Results of in situ hybridization & immunohistochemistry on thin adjacent section to detect expression of HIF-1 alpha1.2 mRNA & HIF1 alpha protein in malignant & benign prostate tissue. In situ hybridization (antisense probe, Fig. 3a) & immunostaining with HIF1 alpha Ab2 (Fig. 3c) on thin adjacent sections of NE-differentiated prostate adenocarcinoma showed co-localization of HIF1 alpha1.2 transcript & HIF-1 alpha protein. Incubation with sense probe did not generate any detectable hybridization signals (Fig. 3b). Both In situ hybridization (Fig. 3d antisense) & HIF-1 alpha Ab2 immunostaining (Fig. 3f) were negative in non-NE-differentiated prostate adenocarcinoma. In situ hybridization with sense probe performed on non-NE-differentiated prostate cancer (Fig. 3e) was negative. In situ hybridizering on benign prostate tissue showed HIF1 alpha1.2 transcript in NE-like cells of benign prostate tissue (Fig. 3g, ↗). The sense probe on thin adjacent section generated no signals (Fig. 3h,↗). Furthermore, co-localization of HIF1 alpha1.2 transcript (Fig. 3i,↗,◢,↖) & HIF1 alpha protein, detected with HIF1 alphaAb3 (Fig. 3j,↗,◢,↖) was also shown in NE-like cells of benign prostate tissue. Panels a, b, c, d, e, f, g, h, i, j: 40× objective. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/20663134), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Immunohistochemistry" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-123" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-123_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-310202415525229.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-123] -" data-caption="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - HX-induced Sirt1 expression in white matter OPCs requires HIF1 alpha.(a) HIF1 alpha stabilization of Sirt1 transcript expression in OPCs as revealed by representative RT–PCR represents higher level of Sirt1 mRNA in VHL cKO mice. GDPDH mRNA serves as a control. Mean±s.e.m., n=3 brains for each group. (b,c) Representative western blot demonstrates a transient increase of HIF1 alpha expression in HX white matter at P18 with no significant effect at P11 (P=0.7955) & P45 (P=0.7333). Histograms show mean±s.e.m. (d,e) Graphs represent the percentages of Sirt1+Ki67+ & NG2+Ki67+ cells after HX white matter in WT & HIF1 alpha KO mice. Number in parentheses within bar indicates number of samples (n=4 brains per group & per genotype; ****P" data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-123" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-123_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-31020241683942.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-123] -" data-caption="Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - Results of in situ hybridization & immunohistochemistry on thin adjacent section to detect expression of HIF-1 alpha1.2 mRNA & HIF1 alpha protein in malignant & benign prostate tissue. In situ hybridization (antisense probe, Fig. 3a) & immunostaining with HIF1 alpha Ab2 (Fig. 3c) on thin adjacent sections of NE-differentiated prostate adenocarcinoma showed co-localization of HIF1 alpha1.2 transcript & HIF-1 alpha protein. Incubation with sense probe did not generate any detectable hybridization signals (Fig. 3b). Both In situ hybridization (Fig. 3d antisense) & HIF-1 alpha Ab2 immunostaining (Fig. 3f) were negative in non-NE-differentiated prostate adenocarcinoma. In situ hybridization with sense probe performed on non-NE-differentiated prostate cancer (Fig. 3e) was negative. In situ hybridizering on benign prostate tissue showed HIF1 alpha1.2 transcript in NE-like cells of benign prostate tissue (Fig. 3g, ↗). The sense probe on thin adjacent section generated no signals (Fig. 3h,↗). Furthermore, co-localization of HIF1 alpha1.2 transcript (Fig. 3i,↗,◢,↖) & HIF1 alpha protein, detected with HIF1 alphaAb3 (Fig. 3j,↗,◢,↖) was also shown in NE-like cells of benign prostate tissue. Panels a, b, c, d, e, f, g, h, i, j: 40× objective. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/20663134), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Immunohistochemistry" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-123" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-123_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-31020241553719.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody (H1alpha67) [NB100-123] -" data-caption="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - HIF-1 alpha expression in primary cortical neurons exposed to hypoxia/ischemia. A & A’) Neurons were double-stained for HIF-1 alpha & MAP2 in the presence of 5 & 25 mM glucose with & without hypoxia. HIF-1 alpha expression in the somata was observed in cells with interneuron-like morphology after hypoxia. B) CoCl2 (0.3 mM) induced HIF-1 alpha expression in cells with interneuron-like morphology. C) Quantification represents the increase in HIF-1 alpha–ir staining (mean ± SD; 5-10 neurons quantified from each experiment, n = 3 experiments). D)In vivo brain slice shows a similar pattern of positive HIF-1 alpha -ir in round soma (open arrow) & negative in neurons with pyramidal-like morphology (solid arrow) in the ipsilateral side. *p " data-badges="" data-application="Immunocytochemistry/ Immunofluorescence" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-123" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-123_mouse-monoclonal-hif-1-alpha-antibody-h1alpha67-3102024161261.jpg" data-alt="HIF-1 alpha Antibody (H1alpha67)" title="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-123] -" data-caption="Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - HX-induced Sirt1 expression in white matter OPCs requires HIF1 alpha.(a) HIF1 alpha stabilization of Sirt1 transcript expression in OPCs as revealed by representative RT–PCR represents higher level of Sirt1 mRNA in VHL cKO mice. GDPDH mRNA serves as a control. Mean±s.e.m., n=3 brains for each group. (b,c) Representative western blot demonstrates a transient increase of HIF1 alpha expression in HX white matter at P18 with no significant effect at P11 (P=0.7955) & P45 (P=0.7333). Histograms show mean±s.e.m. (d,e) Graphs represent the percentages of Sirt1+Ki67+ & NG2+Ki67+ cells after HX white matter in WT & HIF1 alpha KO mice. Number in parentheses within bar indicates number of samples (n=4 brains per group & per genotype; ****P" data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody (H1alpha67), Catalog # NB100-123" data-width="250" data-height="250" class="none"></i> </div> </div> </div> </div> <div class="atc-block-search atc-block-collapsible" data-type="search" data-blockid="ProductCart-NB100-123" > <form action="/commerce/addtocart" method="post" class="px-3 pt-3 add-to-cart-block" data-productcode="NB100-123"> <div class="commerce-atc-full-header atc-header col-four row border-bottom pb-1 m-0"> <div class="commerce-atc-catnum atc-head col-4 col-md-4 font-weight-bold">Catalog #</div> <div class="commerce-atc-availability atc-head col-md-3 font-weight-bold d-none d-md-block">Availability </div> <div class="commerce-atc-price atc-head col-5 col-md-3 font-weight-bold">Size / Price</div> <div class="commerce-atc-quantity atc-head col-3 col-md-2 text-right 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class="col-md-9"> <a href="/p/antibodies/human-mouse-hif-1-alpha-hif1a-pe-conjugated-antibody-241812_ic1935p" title="Human/Mouse HIF-1 alpha/HIF1A PE-conjugated Antibody"> <h4>Human/Mouse HIF-1 alpha/HIF1A PE-conjugated Antibody </h4> </a> </div> <div class="search_result_brand_logo col-md-3 mb-1 d-flex justify-content-xl-end product-brand-logo"> <img alt="R&D Systems, a Bio-Techne brand" src="https://resources.bio-techne.com/bio-techne-assets/images/logos/bio-techne-rnd-systems.svg" class="d-flex w-75"> </div> </div> <div class="row"> <div class="col-md-9"> <div class="mb-1 d-inline-flex"> <div class="align-middle my-1 mr-3 d-block d-md-inline"> Catalog #: <a href="/p/antibodies/human-mouse-hif-1-alpha-hif1a-pe-conjugated-antibody-241812_ic1935p" title="Human/Mouse HIF-1 alpha/HIF1A PE-conjugated Antibody"> IC1935P </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold"> <a href="/p/antibodies/human-mouse-hif-1-alpha-hif1a-pe-conjugated-antibody-241812_ic1935p#citations"><span class="icon-icon-product-citations"></span>Citations (20)</a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold text-nowrap"> <a href="/p/antibodies/human-mouse-hif-1-alpha-hif1a-pe-conjugated-antibody-241812_ic1935p#product-documents"><span class="icon-icon-pdf text-error-red"></span> Product Documents</a> </div> <div class="d-none d-md-inline my-1"> <a href="#" class="search_result_images"><span class="icon-icon-image-camera-plus"></span> Images (1)</a> </div> </div> <div class="d-none d-md-block"> <span class="search_result_applications"></span><span class="applications_title"><strong>Applications:</strong></span> ICFlow </div> <div class="d-none d-md-block"> <span class="species_title"><strong>Reactivity:</strong></span> Human, Mouse </div> </div> <div class="col-md-3"> <button class="py-1 btn-default btn-block atc-view-more collapsed" data-toggle="collapse" href="#collapseButton-9" role="button" aria-expanded="false" aria-controls="collapseButton">View More</button> </div> </div> <div class="commerce-atc-search pt-3 collapse" id="collapseButton-9"> <div class="row"> <div class="search_result_description col-xs-12 col-md-9"> <table class="tablesaw tablesaw-stack mb-2"> <tbody> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Reactivity:</strong></td> <td class="pb-1 w-75 text-break"> Human, Mouse </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Details:</strong></td> <td class="pb-1 w-75 text-break"> Mouse IgG1 Monoclonal Clone #241812 </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Applications:</strong></td> <td class="pb-1 w-75 text-break"> ICFlow </td> </tr> </tbody> </table> <div class="compare_tool_select form-inline mb-2" data-sku="ic1935p"></div> </div> <div class="search_result_image_wrapper col-xs-12 col-md-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="rnd"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src=https://resources.bio-techne.com/images/products/antibody/HIF1_alpha_IC1935P_Flow_Cytometry_13238.jpg data-srcset=https://resources.bio-techne.com/images/products/antibody/HIF1_alpha_IC1935P_Flow_Cytometry_13238.jpg alt="Detection of HIF-1a antibody in MCF-7 Human Cell Line antibody by Flow Cytometry." title="Detection of HIF‑1 alpha in MCF‑7 Human Cell Line by Flow Cytometry." data-caption="MCF-7 human breast cancer cell line treated with CoCl<SUB>2</SUB>was stained with Mouse Anti-Human/Mouse HIF-1a PE-conjugated Monoclonal Antibody (Catalog # IC1935P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." data-badges="<span class="biological-validation"></span>" data-application="Intracellular Staining by Flow Cytometry" data-header="Human/Mouse HIF-1 alpha/HIF1A PE-conjugated Antibody, Catalog # IC1935P" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </div> </div> <div class="atc-block-search atc-block-collapsible" data-type="search" data-blockid="ProductCart-IC1935P" > <form action="/commerce/addtocart" method="post" class="px-3 pt-3 add-to-cart-block" data-productcode="IC1935P"> <div class="commerce-atc-full-header atc-header col-four row border-bottom pb-1 m-0"> <div class="commerce-atc-catnum atc-head col-4 col-md-4 font-weight-bold">Catalog #</div> <div class="commerce-atc-availability atc-head col-md-3 font-weight-bold d-none d-md-block">Availability </div> <div class="commerce-atc-price atc-head col-5 col-md-3 font-weight-bold">Size / Price</div> <div class="commerce-atc-quantity atc-head col-3 col-md-2 text-right font-weight-bold">Qty</div> </div> <div class="spinner-border spinner-border-sm mt-3 ml-3" role="status"> <span class="sr-only">Loading...</span> </div> <div class="d-none search-product-mask"> <div id="ProductCart-IC1935P" class="atc-lines"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="IC1935P"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> IC1935P </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> </div> <div class="atc-control ProductCart-IC1935P-control text-right"> <div class="row d-flex justify-content-end mx-0 p-0"> <div class="order-md-last m___button-atc mx-1"> <button type="submit" value="Add To Cart" class="atc-add-button btn a___button-atc m-1 col-xs-4 order-last btn-md-block w-100"> Add To Cart </button> </div> <div class="m___button-atc mx-1"> <a href="/services/bulk-quotes?product_code=IC1935P" class="atc-bulk-button btn a___button-secondary m-1 col-xs-4 btn-md-block w-100" rel="nofollow">Bulk Order</a> </div> </div> </div> </div> </form> </div> </div> </div> </div> <div class="search_result atc-container pb-3" data-brand="rnd" data-category="Primary Antibodies" data-id="MAB1935" data-name="Human/Mouse HIF-1 alpha/HIF1A Antibody" data-position="10"> <div class="search_details border bg-white p-3 rounded"> <div class="row"> <div class="col-md-9"> <a href="/p/antibodies/human-mouse-hif-1-alpha-hif1a-antibody-241812_mab1935" title="Human/Mouse HIF-1 alpha/HIF1A Antibody"> <h4>Human/Mouse HIF-1 alpha/HIF1A Antibody </h4> </a> </div> <div class="search_result_brand_logo col-md-3 mb-1 d-flex justify-content-xl-end product-brand-logo"> <img alt="R&D Systems, a Bio-Techne brand" src="https://resources.bio-techne.com/bio-techne-assets/images/logos/bio-techne-rnd-systems.svg" class="d-flex w-75"> </div> </div> <div class="row"> <div class="col-md-9"> <div class="mb-1 d-inline-flex"> <div class="align-middle my-1 mr-3 d-block d-md-inline"> Catalog #: <a href="/p/antibodies/human-mouse-hif-1-alpha-hif1a-antibody-241812_mab1935" title="Human/Mouse HIF-1 alpha/HIF1A Antibody"> MAB1935 </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold"> <a href="/p/antibodies/human-mouse-hif-1-alpha-hif1a-antibody-241812_mab1935#citations"><span class="icon-icon-product-citations"></span>Citations (18)</a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold text-nowrap"> <a href="/p/antibodies/human-mouse-hif-1-alpha-hif1a-antibody-241812_mab1935#product-documents"><span class="icon-icon-pdf text-error-red"></span> Product Documents</a> </div> <div class="d-none d-md-inline my-1"> <a href="#" class="search_result_images"><span class="icon-icon-image-camera-plus"></span> Images (1)</a> </div> </div> <div class="d-none d-md-block"> <span class="search_result_applications"></span><span class="applications_title"><strong>Applications:</strong></span> IHC, CyTOF-ready, ICFlow </div> <div class="d-none d-md-block"> <span class="species_title"><strong>Reactivity:</strong></span> Human, Mouse </div> </div> <div class="col-md-3"> <button class="py-1 btn-default btn-block atc-view-more collapsed" data-toggle="collapse" href="#collapseButton-10" role="button" aria-expanded="false" aria-controls="collapseButton">View More</button> </div> </div> <div class="commerce-atc-search pt-3 collapse" id="collapseButton-10"> <div class="row"> <div class="search_result_description col-xs-12 col-md-9"> <table class="tablesaw tablesaw-stack mb-2"> <tbody> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Reactivity:</strong></td> <td class="pb-1 w-75 text-break"> Human, Mouse </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Details:</strong></td> <td class="pb-1 w-75 text-break"> Mouse IgG1 Monoclonal Clone #241812 </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Applications:</strong></td> <td class="pb-1 w-75 text-break"> IHC, CyTOF-ready, ICFlow </td> </tr> </tbody> </table> <div class="compare_tool_select form-inline mb-2" data-sku="mab1935"></div> </div> <div class="search_result_image_wrapper col-xs-12 col-md-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="rnd"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src=https://resources.bio-techne.com/images/products/antibody/HIF-1_alpha_MAB1935_Immunohistochemistry_10047.jpg data-srcset=https://resources.bio-techne.com/images/products/antibody/HIF-1_alpha_MAB1935_Immunohistochemistry_10047.jpg alt="HIF-1 alpha/HIF1A antibody in Human Kidney Cancer Tissue by Immunohistochemistry (IHC-P)." title="HIF-1 alpha/HIF1A in Human Kidney Cancer Tissue." data-caption="HIF-1 alpha/HIF1A was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using Mouse Anti-Human/Mouse HIF-1 alpha/HIF1A Monoclonal Antibody (Catalog # MAB1935) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." data-badges="" data-application="Immunohistochemistry" data-header="Human/Mouse HIF-1 alpha/HIF1A Antibody, Catalog # MAB1935" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </div> </div> <div class="atc-block-search atc-block-collapsible" data-type="search" data-blockid="ProductCart-MAB1935" > <form action="/commerce/addtocart" method="post" class="px-3 pt-3 add-to-cart-block" data-productcode="MAB1935"> <div class="commerce-atc-full-header atc-header col-four row border-bottom pb-1 m-0"> <div class="commerce-atc-catnum atc-head col-4 col-md-4 font-weight-bold">Catalog #</div> <div class="commerce-atc-availability atc-head col-md-3 font-weight-bold d-none d-md-block">Availability </div> <div class="commerce-atc-price atc-head col-5 col-md-3 font-weight-bold">Size / Price</div> <div class="commerce-atc-quantity atc-head col-3 col-md-2 text-right font-weight-bold">Qty</div> </div> <div class="spinner-border spinner-border-sm mt-3 ml-3" role="status"> <span class="sr-only">Loading...</span> </div> <div class="d-none search-product-mask"> <div id="ProductCart-MAB1935" class="atc-lines"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="MAB1935"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> MAB1935 </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="MAB1935-SP"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> MAB1935-SP </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> </div> <div class="atc-control ProductCart-MAB1935-control text-right"> <div class="row d-flex justify-content-end mx-0 p-0"> <div class="order-md-last m___button-atc mx-1"> <button type="submit" value="Add To Cart" class="atc-add-button btn a___button-atc m-1 col-xs-4 order-last btn-md-block w-100"> Add To Cart </button> </div> <div class="m___button-atc mx-1"> <a href="/services/bulk-quotes?product_code=MAB1935" class="atc-bulk-button btn a___button-secondary m-1 col-xs-4 btn-md-block w-100" rel="nofollow">Bulk Order</a> </div> </div> </div> </div> </form> </div> </div> </div> </div> <div class="search_result atc-container pb-3" data-brand="rnd" data-category="Primary Antibodies" data-id="MAB19352" data-name="Human HIF-1 alpha/HIF1A Antibody" data-position="11"> <div class="search_details border bg-white p-3 rounded"> <div class="row"> <div class="col-md-9"> <a href="/p/antibodies/human-hif-1-alpha-hif1a-antibody-2443c_mab19352" title="Human HIF-1 alpha/HIF1A Antibody"> <h4>Human HIF-1 alpha/HIF1A Antibody </h4> </a> </div> <div class="search_result_brand_logo col-md-3 mb-1 d-flex justify-content-xl-end product-brand-logo"> <img alt="R&D Systems, a Bio-Techne brand" src="https://resources.bio-techne.com/bio-techne-assets/images/logos/bio-techne-rnd-systems.svg" class="d-flex w-75"> </div> </div> <div class="row"> <div class="col-md-9"> <div class="mb-1 d-inline-flex"> <div class="align-middle my-1 mr-3 d-block d-md-inline"> Catalog #: <a href="/p/antibodies/human-hif-1-alpha-hif1a-antibody-2443c_mab19352" title="Human HIF-1 alpha/HIF1A Antibody"> MAB19352 </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold text-nowrap"> <a href="/p/antibodies/human-hif-1-alpha-hif1a-antibody-2443c_mab19352#product-documents"><span class="icon-icon-pdf text-error-red"></span> Product Documents</a> </div> <div class="d-none d-md-inline my-1"> <a href="#" class="search_result_images"><span class="icon-icon-image-camera-plus"></span> Images (6)</a> </div> </div> <div class="d-none d-md-block"> <span class="search_result_applications"></span><span class="applications_title"><strong>Applications:</strong></span> WB, Simple Western, IHC, ICC, KO </div> <div class="d-none d-md-block"> <span class="species_title"><strong>Reactivity:</strong></span> Human </div> </div> <div class="col-md-3"> <button class="py-1 btn-default btn-block atc-view-more collapsed" data-toggle="collapse" href="#collapseButton-11" role="button" aria-expanded="false" aria-controls="collapseButton">View More</button> </div> </div> <div class="row"> <div class="align-middle mt-2 col-xs-12 col-sm-9"> <p class="mb-0">Recombinant Monoclonal Antibody.</p> </div> </div> <div class="commerce-atc-search pt-3 collapse" id="collapseButton-11"> <div class="row"> <div class="search_result_description col-xs-12 col-md-9"> <table class="tablesaw tablesaw-stack mb-2"> <tbody> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Reactivity:</strong></td> <td class="pb-1 w-75 text-break"> Human </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Details:</strong></td> <td class="pb-1 w-75 text-break"> Rabbit IgG Monoclonal Clone #2443C </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Applications:</strong></td> <td class="pb-1 w-75 text-break"> WB, Simple Western, IHC, ICC, KO </td> </tr> </tbody> </table> <div class="compare_tool_select form-inline mb-2" data-sku="mab19352"></div> </div> <div class="search_result_image_wrapper col-xs-12 col-md-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="rnd"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src=https://resources.bio-techne.com/images/products/antibody/HIF1_alpha_MAB19352_Western_Blot_23238.jpg data-srcset=https://resources.bio-techne.com/images/products/antibody/HIF1_alpha_MAB19352_Western_Blot_23238.jpg alt="Detection of Human HIF-1 alpha/HIF1A antibody by Western Blot." title="Detection of Human HIF-1 alpha/HIF1A by Western Blot." data-caption="Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 1 mM DFO for overnight. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human HIF-1 alpha/HIF1A Monoclonal Antibody (Catalog # MAB19352) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for HIF-1 alpha/HIF1A at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." data-badges="" data-application="Western Blot" data-header="Human HIF-1 alpha/HIF1A Antibody, Catalog # MAB19352" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> <span class="image-count-text align-middle">(6)</span> </div> </div> </div> <i data-src="https://resources.bio-techne.com/images/products/antibody/HIF1_alpha_MAB19352_Immunocytochemistry__Immunofluorescence_23241.jpg" data-alt="HIF-1 alpha/HIF1A antibody in MCF-7 Human Cell Line by Immunocytochemistry (ICC)." title="HIF-1 alpha/HIF1A in MCF-7 Human Cell Line." data-caption="HIF-1 alpha/HIF1A was detected in immersion fixed MCF-7 human breast cancer cell line using Rabbit Anti-Human HIF-1 alpha/HIF1A Monoclonal Antibody (Catalog # MAB19352) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." data-badges="" data-application="Immunocytochemistry" data-header="Human HIF-1 alpha/HIF1A Antibody, Catalog # MAB19352" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/antibody/HIF1_alpha_MAB19352_Immunocytochemistry__Immunofluorescence_23327.jpg" data-alt="HIF-1 alpha/HIF1A antibody in HeLa Human Cell Line by Immunocytochemistry (ICC)." title="HIF-1 alpha/HIF1A in HeLa Human Cell Line." data-caption="HIF-1 alpha/HIF1A was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line treated with DFO (left panel, positive stain) or HeLa knockouts treated with DFO (right panel, negative stain) using Rabbit Anti-Human HIF-1 alpha/HIF1A Monoclonal Antibody (Catalog # MAB19352) at 1 µg/mL for 3 hours at room temperature. Cells were stained using a Goat anti-Rabbit IgG Secondary Antibody, Alexa Fluor 488 (green) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." data-badges="" data-application="Immunocytochemistry" data-header="Human HIF-1 alpha/HIF1A Antibody, Catalog # MAB19352" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/antibody/HIF1_alpha_MAB19352_Immunohistochemistry_23242.jpg" data-alt="HIF-1 alpha/HIF1A antibody in Human Liver by Immunohistochemistry (IHC-P)." title="HIF-1 alpha/HIF1A in Human Liver." data-caption="HIF-1 alpha/HIF1A was detected in immersion fixed paraffin-embedded sections of human liver using Rabbit Anti-Human HIF-1 alpha/HIF1A Monoclonal Antibody (Catalog # MAB19352) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei in hepatocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." data-badges="" data-application="Immunohistochemistry" data-header="Human HIF-1 alpha/HIF1A Antibody, Catalog # MAB19352" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/antibody/HIF1_alpha_MAB19352_Simple_Western_24084.jpg" data-alt="Detection of Human HIF-1 alpha/HIF1A antibody by Simple WesternTM." title="Detection of Human HIF-1 alpha/HIF1A by Simple Western<sup>TM</sup>." data-caption="Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 1 mM DFO for overnight, loaded at 0.2 mg/mL. A specific band was detected for HIF-1 alpha/HIF1A at approximately 116 kDa (as indicated) using 20 µg/mL of Rabbit Anti-Human HIF-1 alpha/HIF1A Monoclonal Antibody (Catalog # MAB19352) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." data-badges="" data-application="Simple Western" data-header="Human HIF-1 alpha/HIF1A Antibody, Catalog # MAB19352" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/antibody/HIF1_alpha_MAB19352_Knockout_Validated_23239.jpg" data-alt="Western Blot Shows Human HIF-1 alpha/HIF1A Antibody Specificity by Using Knockout Cell Line." title="Western Blot Shows Human HIF-1 alpha/HIF1A Specificity by Using Knockout Cell Line." data-caption="Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HIF-1 alpha/HIF1A knockout HeLa cell line (KO) untreated (-) or treated (+) with 1 mM DFO for overnight. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human HIF-1 alpha/HIF1A Monoclonal Antibody (Catalog # MAB19352) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for HIF-1 alpha/HIF1A at approximately 120 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." data-badges="<span class="knockout-validation"></span>" data-application="Knockout Validated" data-header="Human HIF-1 alpha/HIF1A Antibody, Catalog # MAB19352" data-width="250" data-height="250" class="none"></i> </div> </div> </div> </div> <div class="atc-block-search atc-block-collapsible" data-type="search" data-blockid="ProductCart-MAB19352" > <form action="/commerce/addtocart" method="post" class="px-3 pt-3 add-to-cart-block" data-productcode="MAB19352"> <div class="commerce-atc-full-header atc-header col-four row border-bottom pb-1 m-0"> <div class="commerce-atc-catnum atc-head col-4 col-md-4 font-weight-bold">Catalog #</div> <div class="commerce-atc-availability atc-head col-md-3 font-weight-bold d-none d-md-block">Availability </div> <div class="commerce-atc-price atc-head col-5 col-md-3 font-weight-bold">Size / Price</div> <div class="commerce-atc-quantity atc-head col-3 col-md-2 text-right font-weight-bold">Qty</div> </div> <div class="spinner-border spinner-border-sm mt-3 ml-3" role="status"> <span class="sr-only">Loading...</span> </div> <div class="d-none search-product-mask"> <div id="ProductCart-MAB19352" class="atc-lines"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="MAB19352-100"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> MAB19352-100 </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="MAB19352-SP"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> MAB19352-SP </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> </div> <div class="atc-control ProductCart-MAB19352-control text-right"> <div class="row d-flex justify-content-end mx-0 p-0"> <div class="order-md-last m___button-atc mx-1"> <button type="submit" value="Add To Cart" class="atc-add-button btn a___button-atc m-1 col-xs-4 order-last btn-md-block w-100"> Add To Cart </button> </div> <div class="m___button-atc mx-1"> <a href="/services/bulk-quotes?product_code=MAB19352" class="atc-bulk-button btn a___button-secondary m-1 col-xs-4 btn-md-block w-100" rel="nofollow">Bulk Order</a> </div> </div> </div> </div> </form> </div> </div> </div> </div> <div class="search_result atc-container pb-3" data-brand="rnd" data-category="Primary Antibodies" data-id="IC1935A" data-name="Human/Mouse HIF-1 alpha/HIF1A APC-conjugated Antibody" data-position="12"> <div class="search_details border bg-white p-3 rounded"> <div class="row"> <div class="col-md-9"> <a href="/p/antibodies/human-mouse-hif-1-alpha-hif1a-apc-conjugated-antibody-241812_ic1935a" title="Human/Mouse HIF-1 alpha/HIF1A APC-conjugated Antibody"> <h4>Human/Mouse HIF-1 alpha/HIF1A APC-conjugated Antibody </h4> </a> </div> <div class="search_result_brand_logo col-md-3 mb-1 d-flex justify-content-xl-end product-brand-logo"> <img alt="R&D Systems, a Bio-Techne brand" src="https://resources.bio-techne.com/bio-techne-assets/images/logos/bio-techne-rnd-systems.svg" class="d-flex w-75"> </div> </div> <div class="row"> <div class="col-md-9"> <div class="mb-1 d-inline-flex"> <div class="align-middle my-1 mr-3 d-block d-md-inline"> Catalog #: <a href="/p/antibodies/human-mouse-hif-1-alpha-hif1a-apc-conjugated-antibody-241812_ic1935a" title="Human/Mouse HIF-1 alpha/HIF1A APC-conjugated Antibody"> IC1935A </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold"> <a href="/p/antibodies/human-mouse-hif-1-alpha-hif1a-apc-conjugated-antibody-241812_ic1935a#reviews" class="searchImage_star5"> (2) </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold"> <a href="/p/antibodies/human-mouse-hif-1-alpha-hif1a-apc-conjugated-antibody-241812_ic1935a#citations"><span class="icon-icon-product-citations"></span>Citations (8)</a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold text-nowrap"> <a href="/p/antibodies/human-mouse-hif-1-alpha-hif1a-apc-conjugated-antibody-241812_ic1935a#product-documents"><span class="icon-icon-pdf text-error-red"></span> Product Documents</a> </div> <div class="d-none d-md-inline my-1"> <a href="#" class="search_result_images"><span class="icon-icon-image-camera-plus"></span> Images (1)</a> </div> </div> <div class="d-none d-md-block"> <span class="search_result_applications"></span><span class="applications_title"><strong>Applications:</strong></span> ICFlow </div> <div class="d-none d-md-block"> <span class="species_title"><strong>Reactivity:</strong></span> Human, Mouse </div> </div> <div class="col-md-3"> <button class="py-1 btn-default btn-block atc-view-more collapsed" data-toggle="collapse" href="#collapseButton-12" role="button" aria-expanded="false" aria-controls="collapseButton">View More</button> </div> </div> <div class="commerce-atc-search pt-3 collapse" id="collapseButton-12"> <div class="row"> <div class="search_result_description col-xs-12 col-md-9"> <table class="tablesaw tablesaw-stack mb-2"> <tbody> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Reactivity:</strong></td> <td class="pb-1 w-75 text-break"> Human, Mouse </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Details:</strong></td> <td class="pb-1 w-75 text-break"> Mouse IgG1 Monoclonal Clone #241812 </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Applications:</strong></td> <td class="pb-1 w-75 text-break"> ICFlow </td> </tr> </tbody> </table> <div class="compare_tool_select form-inline mb-2" data-sku="ic1935a"></div> </div> <div class="search_result_image_wrapper col-xs-12 col-md-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="rnd"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src=https://resources.bio-techne.com/images/products/antibody/HIF1_alpha_IC1935A_Flow_Cytometry_13237.jpg data-srcset=https://resources.bio-techne.com/images/products/antibody/HIF1_alpha_IC1935A_Flow_Cytometry_13237.jpg alt="Detection of HIF-1a antibody in MCF-7 Human Cell Line antibody by Flow Cytometry." title="Detection of HIF‑1 alpha in MCF‑7 Human Cell Line by Flow Cytometry." data-caption="MCF-7 human breast cancer cell line treated with CoCl<SUB>2</SUB>was stained with Mouse Anti-Human/Mouse HIF-1a APC-conjugated Monoclonal Antibody (Catalog # IC1935A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." data-badges="<span class="biological-validation"></span>" data-application="Intracellular Staining by Flow Cytometry" data-header="Human/Mouse HIF-1 alpha/HIF1A APC-conjugated Antibody, Catalog # IC1935A" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </div> </div> <div class="atc-block-search atc-block-collapsible" data-type="search" data-blockid="ProductCart-IC1935A" > <form action="/commerce/addtocart" method="post" class="px-3 pt-3 add-to-cart-block" data-productcode="IC1935A"> <div class="commerce-atc-full-header atc-header col-four row border-bottom pb-1 m-0"> <div class="commerce-atc-catnum atc-head col-4 col-md-4 font-weight-bold">Catalog #</div> <div class="commerce-atc-availability atc-head col-md-3 font-weight-bold d-none d-md-block">Availability </div> <div class="commerce-atc-price atc-head col-5 col-md-3 font-weight-bold">Size / Price</div> <div class="commerce-atc-quantity atc-head col-3 col-md-2 text-right font-weight-bold">Qty</div> </div> <div class="spinner-border spinner-border-sm mt-3 ml-3" role="status"> <span class="sr-only">Loading...</span> </div> <div class="d-none search-product-mask"> <div id="ProductCart-IC1935A" class="atc-lines"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="IC1935A"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> IC1935A </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> </div> <div class="atc-control ProductCart-IC1935A-control text-right"> <div class="row d-flex justify-content-end mx-0 p-0"> <div class="order-md-last m___button-atc mx-1"> <button type="submit" value="Add To Cart" class="atc-add-button btn a___button-atc m-1 col-xs-4 order-last btn-md-block w-100"> Add To Cart </button> </div> <div class="m___button-atc mx-1"> <a href="/services/bulk-quotes?product_code=IC1935A" class="atc-bulk-button btn a___button-secondary m-1 col-xs-4 btn-md-block w-100" rel="nofollow">Bulk Order</a> </div> </div> </div> </div> </form> </div> </div> </div> </div> <div class="search_result atc-container pb-3" data-brand="rnd" data-category="Primary Antibodies" data-id="MAB19351" data-name="Human HIF-1 alpha/HIF1A Antibody" data-position="13"> <div class="search_details border bg-white p-3 rounded"> <div class="row"> <div class="col-md-9"> <a href="/p/antibodies/human-hif-1-alpha-hif1a-antibody-2443b_mab19351" title="Human HIF-1 alpha/HIF1A Antibody"> <h4>Human HIF-1 alpha/HIF1A Antibody </h4> </a> </div> <div class="search_result_brand_logo col-md-3 mb-1 d-flex justify-content-xl-end product-brand-logo"> <img alt="R&D Systems, a Bio-Techne brand" src="https://resources.bio-techne.com/bio-techne-assets/images/logos/bio-techne-rnd-systems.svg" class="d-flex w-75"> </div> </div> <div class="row"> <div class="col-md-9"> <div class="mb-1 d-inline-flex"> <div class="align-middle my-1 mr-3 d-block d-md-inline"> Catalog #: <a href="/p/antibodies/human-hif-1-alpha-hif1a-antibody-2443b_mab19351" title="Human HIF-1 alpha/HIF1A Antibody"> MAB19351 </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold"> <a href="/p/antibodies/human-hif-1-alpha-hif1a-antibody-2443b_mab19351#citations"><span class="icon-icon-product-citations"></span>Citations (1)</a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold text-nowrap"> <a href="/p/antibodies/human-hif-1-alpha-hif1a-antibody-2443b_mab19351#product-documents"><span class="icon-icon-pdf text-error-red"></span> Product Documents</a> </div> <div class="d-none d-md-inline my-1"> <a href="#" class="search_result_images"><span class="icon-icon-image-camera-plus"></span> Images (4)</a> </div> </div> <div class="d-none d-md-block"> <span class="search_result_applications"></span><span class="applications_title"><strong>Applications:</strong></span> WB, Simple Western, ICC, KO </div> <div class="d-none d-md-block"> <span class="species_title"><strong>Reactivity:</strong></span> Human </div> </div> <div class="col-md-3"> <button class="py-1 btn-default btn-block atc-view-more collapsed" data-toggle="collapse" href="#collapseButton-13" role="button" aria-expanded="false" aria-controls="collapseButton">View More</button> </div> </div> <div class="row"> <div class="align-middle mt-2 col-xs-12 col-sm-9"> <p class="mb-0">Recombinant Monoclonal Antibody.</p> </div> </div> <div class="commerce-atc-search pt-3 collapse" id="collapseButton-13"> <div class="row"> <div class="search_result_description col-xs-12 col-md-9"> <table class="tablesaw tablesaw-stack mb-2"> <tbody> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Reactivity:</strong></td> <td class="pb-1 w-75 text-break"> Human </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Details:</strong></td> <td class="pb-1 w-75 text-break"> Rabbit IgG1 Monoclonal Clone #2443B </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Applications:</strong></td> <td class="pb-1 w-75 text-break"> WB, Simple Western, ICC, KO </td> </tr> </tbody> </table> <div class="compare_tool_select form-inline mb-2" data-sku="mab19351"></div> </div> <div class="search_result_image_wrapper col-xs-12 col-md-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="rnd"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src=https://resources.bio-techne.com/images/products/antibody/HIF1_alpha_MAB19351_Western_Blot_23177.jpg data-srcset=https://resources.bio-techne.com/images/products/antibody/HIF1_alpha_MAB19351_Western_Blot_23177.jpg alt="Detection of Human HIF-1 alpha/HIF1A antibody by Western Blot." title="Detection of Human HIF-1 alpha/HIF1A by Western Blot." data-caption="Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 1 mM DFO for overnight. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human HIF-1 alpha/HIF1A Monoclonal Antibody (Catalog # MAB19351) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for HIF-1 alpha/HIF1A at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." data-badges="" data-application="Western Blot" data-header="Human HIF-1 alpha/HIF1A Antibody, Catalog # MAB19351" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> <span class="image-count-text align-middle">(4)</span> </div> </div> </div> <i data-src="https://resources.bio-techne.com/images/products/antibody/HIF1_alpha_MAB19351_Immunocytochemistry__Immunofluorescence_23128.jpg" data-alt="HIF-1 alpha/HIF1A antibody in HeLa Human Cell Line by Immunocytochemistry (ICC)." title="HIF-1 alpha/HIF1A in HeLa Human Cell Line." data-caption="HIF-1 alpha/HIF1A was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line treated with DFO (left panel) or untreated (right panel) using Rabbit Anti-Human HIF-1 alpha/HIF1A Monoclonal Antibody (Catalog # MAB19351) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." data-badges="" data-application="Immunocytochemistry" data-header="Human HIF-1 alpha/HIF1A Antibody, Catalog # MAB19351" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/antibody/HIF1_alpha_MAB19351_Simple_Western_23413.jpg" data-alt="Detection of Human HIF-1 alpha/HIF1A antibody by Simple WesternTM." title="Detection of Human HIF-1 alpha/HIF1A by Simple Western<sup>TM</sup>." data-caption="Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 1 mM DFO for overnight, loaded at 0.2 mg/mL. A specific band was detected for HIF-1 alpha/HIF1A at approximately 116 kDa (as indicated) using 20 µg/mL of Rabbit Anti-Human HIF-1 alpha/HIF1A Monoclonal Antibody (Catalog # MAB19351) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." data-badges="" data-application="Simple Western" data-header="Human HIF-1 alpha/HIF1A Antibody, Catalog # MAB19351" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/antibody/HIF1_alpha_MAB19351_Western_Blot_23095.jpg" data-alt="Western Blot Shows Human HIF-1 alpha/HIF1A Antibody Specificity by Using Knockout Cell Line." title="Western Blot Shows Human HIF-1 alpha/HIF1A Specificity by Using Knockout Cell Line." data-caption="Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HIF-1 alpha/HIF1A knockout HeLa cell line (KO) untreated (-) or treated (+) with 1 mM DFO for overnight. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human HIF-1 alpha/HIF1A Monoclonal Antibody (Catalog # MAB19351) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HIF-1 alpha/HIF1A at approximately 120 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control.This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." data-badges="<span class="knockout-validation"></span>" data-application="Knockout Validated" data-header="Human HIF-1 alpha/HIF1A Antibody, Catalog # MAB19351" data-width="250" data-height="250" class="none"></i> </div> </div> </div> </div> <div class="atc-block-search atc-block-collapsible" data-type="search" data-blockid="ProductCart-MAB19351" > <form action="/commerce/addtocart" method="post" class="px-3 pt-3 add-to-cart-block" data-productcode="MAB19351"> <div class="commerce-atc-full-header atc-header col-four row border-bottom pb-1 m-0"> <div class="commerce-atc-catnum atc-head col-4 col-md-4 font-weight-bold">Catalog #</div> <div class="commerce-atc-availability atc-head col-md-3 font-weight-bold d-none d-md-block">Availability </div> <div class="commerce-atc-price atc-head col-5 col-md-3 font-weight-bold">Size / Price</div> <div class="commerce-atc-quantity atc-head col-3 col-md-2 text-right font-weight-bold">Qty</div> </div> <div class="spinner-border spinner-border-sm mt-3 ml-3" role="status"> <span class="sr-only">Loading...</span> </div> <div class="d-none search-product-mask"> <div id="ProductCart-MAB19351" class="atc-lines"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="MAB19351-100"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> MAB19351-100 </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="MAB19351-SP"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> MAB19351-SP </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> </div> <div class="atc-control ProductCart-MAB19351-control text-right"> <div class="row d-flex justify-content-end mx-0 p-0"> <div class="order-md-last m___button-atc mx-1"> <button type="submit" value="Add To Cart" class="atc-add-button btn a___button-atc m-1 col-xs-4 order-last btn-md-block w-100"> Add To Cart </button> </div> <div class="m___button-atc mx-1"> <a href="/services/bulk-quotes?product_code=MAB19351" class="atc-bulk-button btn a___button-secondary m-1 col-xs-4 btn-md-block w-100" rel="nofollow">Bulk Order</a> </div> </div> </div> </div> </form> </div> </div> </div> </div> <div class="search_result atc-container pb-3" data-brand="rnd" data-category="Primary Antibodies" data-id="IC1935T" data-name="Human/Mouse HIF-1 alpha/HIF1A Alexa&nbsp;Fluor®&nbsp;594-conjugated Antibody" data-position="14"> <div class="search_details border bg-white p-3 rounded"> <div class="row"> <div class="col-md-9"> <a href="/p/antibodies/human-mouse-hif-1-alpha-hif1a-alexa-fluor-594-conjugated-antibody-241812_ic1935t" title="Human/Mouse HIF-1 alpha/HIF1A Alexa&nbsp;Fluor®&nbsp;594-conjugated Antibody"> <h4>Human/Mouse HIF-1 alpha/HIF1A Alexa Fluor® 594-conjugated Antibody </h4> </a> </div> <div class="search_result_brand_logo col-md-3 mb-1 d-flex justify-content-xl-end product-brand-logo"> <img alt="R&D Systems, a Bio-Techne brand" src="https://resources.bio-techne.com/bio-techne-assets/images/logos/bio-techne-rnd-systems.svg" class="d-flex w-75"> </div> </div> <div class="row"> <div class="col-md-9"> <div class="mb-1 d-inline-flex"> <div class="align-middle my-1 mr-3 d-block d-md-inline"> Catalog #: <a href="/p/antibodies/human-mouse-hif-1-alpha-hif1a-alexa-fluor-594-conjugated-antibody-241812_ic1935t" title="Human/Mouse HIF-1 alpha/HIF1A Alexa&nbsp;Fluor®&nbsp;594-conjugated Antibody"> IC1935T </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold text-nowrap"> <a href="/p/antibodies/human-mouse-hif-1-alpha-hif1a-alexa-fluor-594-conjugated-antibody-241812_ic1935t#product-documents"><span class="icon-icon-pdf text-error-red"></span> Product Documents</a> </div> </div> <div class="d-none d-md-block"> <span class="search_result_applications"></span><span class="applications_title"><strong>Applications:</strong></span> ICFlow </div> <div class="d-none d-md-block"> <span class="species_title"><strong>Reactivity:</strong></span> Human, Mouse </div> </div> <div class="col-md-3"> <button class="py-1 btn-default btn-block atc-view-more collapsed" data-toggle="collapse" href="#collapseButton-14" role="button" aria-expanded="false" aria-controls="collapseButton">View More</button> </div> </div> <div class="commerce-atc-search pt-3 collapse" id="collapseButton-14"> <div class="row"> <div class="search_result_description col-xs-12 col-sm-12"> </div> </div> <div class="row"> <div class="search_result_product_details col-xs-12 col-sm-9"> <table class="tablesaw tablesaw-stack mb-2"> <tbody> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Reactivity:</strong></td> <td class="pb-1 w-75 text-break"> Human, Mouse </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Details:</strong></td> <td class="pb-1 w-75 text-break"> Mouse IgG1 Monoclonal Clone #241812 </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Applications:</strong></td> <td class="pb-1 w-75 text-break"> ICFlow </td> </tr> </tbody> </table> <div class="compare_tool_select form-inline mb-2" data-sku="ic1935t"></div> </div> </div> <div class="atc-block-search atc-block-collapsible" data-type="search" data-blockid="ProductCart-IC1935T" > <form action="/commerce/addtocart" method="post" class="px-3 pt-3 add-to-cart-block" data-productcode="IC1935T"> <div class="commerce-atc-full-header atc-header col-four row border-bottom pb-1 m-0"> <div class="commerce-atc-catnum atc-head col-4 col-md-4 font-weight-bold">Catalog #</div> <div class="commerce-atc-availability atc-head col-md-3 font-weight-bold d-none d-md-block">Availability </div> <div class="commerce-atc-price atc-head col-5 col-md-3 font-weight-bold">Size / Price</div> <div class="commerce-atc-quantity atc-head col-3 col-md-2 text-right font-weight-bold">Qty</div> </div> <div class="spinner-border spinner-border-sm mt-3 ml-3" role="status"> <span class="sr-only">Loading...</span> </div> <div class="d-none search-product-mask"> <div id="ProductCart-IC1935T" class="atc-lines"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="IC1935T-100UG"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> IC1935T-100UG </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> </div> <div class="atc-control ProductCart-IC1935T-control text-right"> <div class="row d-flex justify-content-end mx-0 p-0"> <div class="order-md-last m___button-atc mx-1"> <button type="submit" value="Add To Cart" class="atc-add-button btn a___button-atc m-1 col-xs-4 order-last btn-md-block w-100"> Add To Cart </button> </div> <div class="m___button-atc mx-1"> <a href="/services/bulk-quotes?product_code=IC1935T" class="atc-bulk-button btn a___button-secondary m-1 col-xs-4 btn-md-block w-100" rel="nofollow">Bulk Order</a> </div> </div> </div> </div> </form> </div> </div> </div> </div> <div class="search_result atc-container pb-3" data-brand="rnd" data-category="Primary Antibodies" data-id="IC1935C" data-name="Human/Mouse HIF-1 alpha/HIF1A PerCP-conjugated Antibody" data-position="15"> <div class="search_details border bg-white p-3 rounded"> <div class="row"> <div class="col-md-9"> <a href="/p/antibodies/human-mouse-hif-1-alpha-hif1a-percp-conjugated-antibody-241812_ic1935c" title="Human/Mouse HIF-1 alpha/HIF1A PerCP-conjugated Antibody"> <h4>Human/Mouse HIF-1 alpha/HIF1A PerCP-conjugated Antibody </h4> </a> </div> <div class="search_result_brand_logo col-md-3 mb-1 d-flex justify-content-xl-end product-brand-logo"> <img alt="R&D Systems, a Bio-Techne brand" src="https://resources.bio-techne.com/bio-techne-assets/images/logos/bio-techne-rnd-systems.svg" class="d-flex w-75"> </div> </div> <div class="row"> <div class="col-md-9"> <div class="mb-1 d-inline-flex"> <div class="align-middle my-1 mr-3 d-block d-md-inline"> Catalog #: <a href="/p/antibodies/human-mouse-hif-1-alpha-hif1a-percp-conjugated-antibody-241812_ic1935c" title="Human/Mouse HIF-1 alpha/HIF1A PerCP-conjugated Antibody"> IC1935C </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold"> <a href="/p/antibodies/human-mouse-hif-1-alpha-hif1a-percp-conjugated-antibody-241812_ic1935c#citations"><span class="icon-icon-product-citations"></span>Citations (1)</a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold text-nowrap"> <a href="/p/antibodies/human-mouse-hif-1-alpha-hif1a-percp-conjugated-antibody-241812_ic1935c#product-documents"><span class="icon-icon-pdf text-error-red"></span> Product Documents</a> </div> <div class="d-none d-md-inline my-1"> <a href="#" class="search_result_images"><span class="icon-icon-image-camera-plus"></span> Images (2)</a> </div> </div> <div class="d-none d-md-block"> <span class="search_result_applications"></span><span class="applications_title"><strong>Applications:</strong></span> ICFlow </div> <div class="d-none d-md-block"> <span class="species_title"><strong>Reactivity:</strong></span> Human, Mouse </div> </div> <div class="col-md-3"> <button class="py-1 btn-default btn-block atc-view-more collapsed" data-toggle="collapse" href="#collapseButton-15" role="button" aria-expanded="false" aria-controls="collapseButton">View More</button> </div> </div> <div class="commerce-atc-search pt-3 collapse" id="collapseButton-15"> <div class="row"> <div class="search_result_description col-xs-12 col-md-9"> <table class="tablesaw tablesaw-stack mb-2"> <tbody> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Reactivity:</strong></td> <td class="pb-1 w-75 text-break"> Human, Mouse </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Details:</strong></td> <td class="pb-1 w-75 text-break"> Mouse IgG1 Monoclonal Clone #241812 </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Applications:</strong></td> <td class="pb-1 w-75 text-break"> ICFlow </td> </tr> </tbody> </table> <div class="compare_tool_select form-inline mb-2" data-sku="ic1935c"></div> </div> <div class="search_result_image_wrapper col-xs-12 col-md-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="rnd"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src=https://resources.bio-techne.com/images/products/antibody/HIF1_alpha_IC1935C_Flow_Cytometry_14086.jpg data-srcset=https://resources.bio-techne.com/images/products/antibody/HIF1_alpha_IC1935C_Flow_Cytometry_14086.jpg alt="Detection of HIF-1a antibody in MCF-7 Human Cell Line antibody by Flow Cytometry." title="Detection of HIF‑1 alpha in MCF‑7 Human Cell Line by Flow Cytometry." data-caption="MCF-7 human breast cancer cell line treated with CoCl<SUB>2</SUB>was stained with Mouse Anti-Human/Mouse HIF-1a PerCP-conjugated Monoclonal Antibody (Catalog # IC1935C, filled histogram) or isotype control antibody (Catalog # IC002C, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." data-badges="<span class="biological-validation"></span>" data-application="Intracellular Staining by Flow Cytometry" data-header="Human/Mouse HIF-1 alpha/HIF1A PerCP-conjugated Antibody, Catalog # IC1935C" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> <span class="image-count-text align-middle">(2)</span> </div> </div> </div> <i data-src="https://resources.bio-techne.com/images/products/ic1935c_human-mouse-hif-1-alpha-percp-mab-clone-241812-intracellular-staining-by-flow-cytometry-293202119222.jpg" data-alt="" title="Detection of HIF‑1 alpha in MCF‑7 Human Cell Line by Flow Cytometry." data-caption="MCF-7 human breast cancer cell line treated with 1 mM DFO overnight was stained with Mouse Anti-Human/Mouse HIF-1a PerCP-conjugated Monoclonal Antibody (Catalog # IC1935C, filled histogram) or isotype control antibody (IC002C, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with Flow Cytometry FoxP3/Transcription Factor Fixation & Perm Kit (FC012). Staining was performed using our Staining Intracellular Molecules protocol." data-badges="<span class="biological-validation"></span>" data-application="Intracellular Staining by Flow Cytometry" data-header="Human/Mouse HIF-1 alpha/HIF1A PerCP-conjugated Antibody, Catalog # IC1935C" data-width="250" data-height="250" class="none"></i> </div> </div> </div> </div> <div class="atc-block-search atc-block-collapsible" data-type="search" data-blockid="ProductCart-IC1935C" > <form action="/commerce/addtocart" method="post" class="px-3 pt-3 add-to-cart-block" data-productcode="IC1935C"> <div class="commerce-atc-full-header atc-header col-four row border-bottom pb-1 m-0"> <div class="commerce-atc-catnum atc-head col-4 col-md-4 font-weight-bold">Catalog #</div> <div class="commerce-atc-availability atc-head col-md-3 font-weight-bold d-none d-md-block">Availability </div> <div class="commerce-atc-price atc-head col-5 col-md-3 font-weight-bold">Size / Price</div> <div class="commerce-atc-quantity atc-head col-3 col-md-2 text-right font-weight-bold">Qty</div> </div> <div class="spinner-border spinner-border-sm mt-3 ml-3" role="status"> <span class="sr-only">Loading...</span> </div> <div class="d-none search-product-mask"> <div id="ProductCart-IC1935C" class="atc-lines"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="IC1935C"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> IC1935C </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> </div> <div class="atc-control ProductCart-IC1935C-control text-right"> <div class="row d-flex justify-content-end mx-0 p-0"> <div class="order-md-last m___button-atc mx-1"> <button type="submit" value="Add To Cart" class="atc-add-button btn a___button-atc m-1 col-xs-4 order-last btn-md-block w-100"> Add To Cart </button> </div> <div class="m___button-atc mx-1"> <a href="/services/bulk-quotes?product_code=IC1935C" class="atc-bulk-button btn a___button-secondary m-1 col-xs-4 btn-md-block w-100" rel="nofollow">Bulk Order</a> </div> </div> </div> </div> </form> </div> </div> </div> </div> <div class="search_result atc-container pb-3" data-brand="rnd" data-category="Primary Antibodies" data-id="IC1935N" data-name="Human/Mouse HIF-1 alpha/HIF1A Alexa&nbsp;Fluor®&nbsp;700-conjugated Antibody" data-position="16"> <div class="search_details border bg-white p-3 rounded"> <div class="row"> <div class="col-md-9"> <a href="/p/antibodies/human-mouse-hif-1-alpha-hif1a-alexa-fluor-700-conjugated-antibody-241812_ic1935n" title="Human/Mouse HIF-1 alpha/HIF1A Alexa&nbsp;Fluor®&nbsp;700-conjugated Antibody"> <h4>Human/Mouse HIF-1 alpha/HIF1A Alexa Fluor® 700-conjugated Antibody </h4> </a> </div> <div class="search_result_brand_logo col-md-3 mb-1 d-flex justify-content-xl-end product-brand-logo"> <img alt="R&D Systems, a Bio-Techne brand" src="https://resources.bio-techne.com/bio-techne-assets/images/logos/bio-techne-rnd-systems.svg" class="d-flex w-75"> </div> </div> <div class="row"> <div class="col-md-9"> <div class="mb-1 d-inline-flex"> <div class="align-middle my-1 mr-3 d-block d-md-inline"> Catalog #: <a href="/p/antibodies/human-mouse-hif-1-alpha-hif1a-alexa-fluor-700-conjugated-antibody-241812_ic1935n" title="Human/Mouse HIF-1 alpha/HIF1A Alexa&nbsp;Fluor®&nbsp;700-conjugated Antibody"> IC1935N </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold"> <a href="/p/antibodies/human-mouse-hif-1-alpha-hif1a-alexa-fluor-700-conjugated-antibody-241812_ic1935n#reviews" class="searchImage_star5"> (1) </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold"> <a href="/p/antibodies/human-mouse-hif-1-alpha-hif1a-alexa-fluor-700-conjugated-antibody-241812_ic1935n#citations"><span class="icon-icon-product-citations"></span>Citations (1)</a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold text-nowrap"> <a href="/p/antibodies/human-mouse-hif-1-alpha-hif1a-alexa-fluor-700-conjugated-antibody-241812_ic1935n#product-documents"><span class="icon-icon-pdf text-error-red"></span> Product Documents</a> </div> <div class="d-none d-md-inline my-1"> <a href="#" class="search_result_images"><span class="icon-icon-image-camera-plus"></span> Images (1)</a> </div> </div> <div class="d-none d-md-block"> <span class="search_result_applications"></span><span class="applications_title"><strong>Applications:</strong></span> ICFlow </div> <div class="d-none d-md-block"> <span class="species_title"><strong>Reactivity:</strong></span> Human, Mouse </div> </div> <div class="col-md-3"> <button class="py-1 btn-default btn-block atc-view-more collapsed" data-toggle="collapse" href="#collapseButton-16" role="button" aria-expanded="false" aria-controls="collapseButton">View More</button> </div> </div> <div class="commerce-atc-search pt-3 collapse" id="collapseButton-16"> <div class="row"> <div class="search_result_description col-xs-12 col-md-9"> <table class="tablesaw tablesaw-stack mb-2"> <tbody> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Reactivity:</strong></td> <td class="pb-1 w-75 text-break"> Human, Mouse </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Details:</strong></td> <td class="pb-1 w-75 text-break"> Mouse IgG1 Monoclonal Clone #241812 </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Applications:</strong></td> <td class="pb-1 w-75 text-break"> ICFlow </td> </tr> </tbody> </table> <div class="compare_tool_select form-inline mb-2" data-sku="ic1935n"></div> </div> <div class="search_result_image_wrapper col-xs-12 col-md-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="rnd"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src=https://resources.bio-techne.com/images/products/antibody/HIF1_alpha_IC1935N_Flow_Cytometry_14087.jpg data-srcset=https://resources.bio-techne.com/images/products/antibody/HIF1_alpha_IC1935N_Flow_Cytometry_14087.jpg alt="Detection of HIF-1a antibody in MCF-7 Human Cell Line antibody by Flow Cytometry." title="Detection of HIF‑1 alpha in MCF‑7 Human Cell Line by Flow Cytometry." data-caption="MCF-7 human breast cancer cell line treated with CoCl<SUB>2</SUB>was stained with Mouse Anti-Human/Mouse HIF-1a Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # IC1935N, filled histogram) or isotype control antibody (Catalog # IC002N, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." data-badges="<span class="biological-validation"></span>" data-application="Intracellular Staining by Flow Cytometry" data-header="Human/Mouse HIF-1 alpha/HIF1A Alexa&nbsp;Fluor®&nbsp;700-conjugated Antibody, Catalog # IC1935N" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </div> </div> <div class="atc-block-search atc-block-collapsible" data-type="search" data-blockid="ProductCart-IC1935N" > <form action="/commerce/addtocart" method="post" class="px-3 pt-3 add-to-cart-block" data-productcode="IC1935N"> <div class="commerce-atc-full-header atc-header col-four row border-bottom pb-1 m-0"> <div class="commerce-atc-catnum atc-head col-4 col-md-4 font-weight-bold">Catalog #</div> <div class="commerce-atc-availability atc-head col-md-3 font-weight-bold d-none d-md-block">Availability </div> <div class="commerce-atc-price atc-head col-5 col-md-3 font-weight-bold">Size / Price</div> <div class="commerce-atc-quantity atc-head col-3 col-md-2 text-right font-weight-bold">Qty</div> </div> <div class="spinner-border spinner-border-sm mt-3 ml-3" role="status"> <span class="sr-only">Loading...</span> </div> <div class="d-none search-product-mask"> <div id="ProductCart-IC1935N" class="atc-lines"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="IC1935N"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> IC1935N </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> </div> <div class="atc-control ProductCart-IC1935N-control text-right"> <div class="row d-flex justify-content-end mx-0 p-0"> <div class="order-md-last m___button-atc mx-1"> <button type="submit" value="Add To Cart" class="atc-add-button btn a___button-atc m-1 col-xs-4 order-last btn-md-block w-100"> Add To Cart </button> </div> <div class="m___button-atc mx-1"> <a href="/services/bulk-quotes?product_code=IC1935N" class="atc-bulk-button btn a___button-secondary m-1 col-xs-4 btn-md-block w-100" rel="nofollow">Bulk Order</a> </div> </div> </div> </div> </form> </div> </div> </div> </div> <div class="search_result atc-container pb-3" data-brand="rnd" data-category="Primary Antibodies" data-id="IC1935R" data-name="Human/Mouse HIF-1 alpha/HIF1A Alexa&nbsp;Fluor®&nbsp;647-conjugated Antibody" data-position="17"> <div class="search_details border bg-white p-3 rounded"> <div class="row"> <div class="col-md-9"> <a href="/p/antibodies/human-mouse-hif-1-alpha-hif1a-alexa-fluor-647-conjugated-antibody-241812_ic1935r" title="Human/Mouse HIF-1 alpha/HIF1A Alexa&nbsp;Fluor®&nbsp;647-conjugated Antibody"> <h4>Human/Mouse HIF-1 alpha/HIF1A Alexa Fluor® 647-conjugated Antibody </h4> </a> </div> <div class="search_result_brand_logo col-md-3 mb-1 d-flex justify-content-xl-end product-brand-logo"> <img alt="R&D Systems, a Bio-Techne brand" src="https://resources.bio-techne.com/bio-techne-assets/images/logos/bio-techne-rnd-systems.svg" class="d-flex w-75"> </div> </div> <div class="row"> <div class="col-md-9"> <div class="mb-1 d-inline-flex"> <div class="align-middle my-1 mr-3 d-block d-md-inline"> Catalog #: <a href="/p/antibodies/human-mouse-hif-1-alpha-hif1a-alexa-fluor-647-conjugated-antibody-241812_ic1935r" title="Human/Mouse HIF-1 alpha/HIF1A Alexa&nbsp;Fluor®&nbsp;647-conjugated Antibody"> IC1935R </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold"> <a href="/p/antibodies/human-mouse-hif-1-alpha-hif1a-alexa-fluor-647-conjugated-antibody-241812_ic1935r#reviews" class="searchImage_star1"> (1) </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold"> <a href="/p/antibodies/human-mouse-hif-1-alpha-hif1a-alexa-fluor-647-conjugated-antibody-241812_ic1935r#citations"><span class="icon-icon-product-citations"></span>Citations (1)</a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold text-nowrap"> <a href="/p/antibodies/human-mouse-hif-1-alpha-hif1a-alexa-fluor-647-conjugated-antibody-241812_ic1935r#product-documents"><span class="icon-icon-pdf text-error-red"></span> Product Documents</a> </div> </div> <div class="d-none d-md-block"> <span class="search_result_applications"></span><span class="applications_title"><strong>Applications:</strong></span> ICFlow </div> <div class="d-none d-md-block"> <span class="species_title"><strong>Reactivity:</strong></span> Human, Mouse </div> </div> <div class="col-md-3"> <button class="py-1 btn-default btn-block atc-view-more collapsed" data-toggle="collapse" href="#collapseButton-17" role="button" aria-expanded="false" aria-controls="collapseButton">View More</button> </div> </div> <div class="commerce-atc-search pt-3 collapse" id="collapseButton-17"> <div class="row"> <div class="search_result_description col-xs-12 col-sm-12"> </div> </div> <div class="row"> <div class="search_result_product_details col-xs-12 col-sm-9"> <table class="tablesaw tablesaw-stack mb-2"> <tbody> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Reactivity:</strong></td> <td class="pb-1 w-75 text-break"> Human, Mouse </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Details:</strong></td> <td class="pb-1 w-75 text-break"> Mouse IgG1 Monoclonal Clone #241812 </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Applications:</strong></td> <td class="pb-1 w-75 text-break"> ICFlow </td> </tr> </tbody> </table> <div class="compare_tool_select form-inline mb-2" data-sku="ic1935r"></div> </div> </div> <div class="atc-block-search atc-block-collapsible" data-type="search" data-blockid="ProductCart-IC1935R" > <form action="/commerce/addtocart" method="post" class="px-3 pt-3 add-to-cart-block" data-productcode="IC1935R"> <div class="commerce-atc-full-header atc-header col-four row border-bottom pb-1 m-0"> <div class="commerce-atc-catnum atc-head col-4 col-md-4 font-weight-bold">Catalog #</div> <div class="commerce-atc-availability atc-head col-md-3 font-weight-bold d-none d-md-block">Availability </div> <div class="commerce-atc-price atc-head col-5 col-md-3 font-weight-bold">Size / Price</div> <div class="commerce-atc-quantity atc-head col-3 col-md-2 text-right font-weight-bold">Qty</div> </div> <div class="spinner-border spinner-border-sm mt-3 ml-3" role="status"> <span class="sr-only">Loading...</span> </div> <div class="d-none search-product-mask"> <div id="ProductCart-IC1935R" class="atc-lines"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="IC1935R-100UG"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> IC1935R-100UG </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> </div> <div class="atc-control ProductCart-IC1935R-control text-right"> <div class="row d-flex justify-content-end mx-0 p-0"> <div class="order-md-last m___button-atc mx-1"> <button type="submit" value="Add To Cart" class="atc-add-button btn a___button-atc m-1 col-xs-4 order-last btn-md-block w-100"> Add To Cart </button> </div> <div class="m___button-atc mx-1"> <a href="/services/bulk-quotes?product_code=IC1935R" class="atc-bulk-button btn a___button-secondary m-1 col-xs-4 btn-md-block w-100" rel="nofollow">Bulk Order</a> </div> </div> </div> </div> </form> </div> </div> </div> </div> <div class="search_result atc-container pb-3" data-brand="rnd" data-category="Primary Antibodies" data-id="IC1935V" data-name="Human/Mouse HIF-1 alpha/HIF1A Alexa&nbsp;Fluor®&nbsp;405-conjugated Antibody" data-position="18"> <div class="search_details border bg-white p-3 rounded"> <div class="row"> <div class="col-md-9"> <a href="/p/antibodies/human-mouse-hif-1-alpha-hif1a-alexa-fluor-405-conjugated-antibody-241812_ic1935v" title="Human/Mouse HIF-1 alpha/HIF1A Alexa&nbsp;Fluor®&nbsp;405-conjugated Antibody"> <h4>Human/Mouse HIF-1 alpha/HIF1A Alexa Fluor® 405-conjugated Antibody </h4> </a> </div> <div class="search_result_brand_logo col-md-3 mb-1 d-flex justify-content-xl-end product-brand-logo"> <img alt="R&D Systems, a Bio-Techne brand" src="https://resources.bio-techne.com/bio-techne-assets/images/logos/bio-techne-rnd-systems.svg" class="d-flex w-75"> </div> </div> <div class="row"> <div class="col-md-9"> <div class="mb-1 d-inline-flex"> <div class="align-middle my-1 mr-3 d-block d-md-inline"> Catalog #: <a href="/p/antibodies/human-mouse-hif-1-alpha-hif1a-alexa-fluor-405-conjugated-antibody-241812_ic1935v" title="Human/Mouse HIF-1 alpha/HIF1A Alexa&nbsp;Fluor®&nbsp;405-conjugated Antibody"> IC1935V </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold text-nowrap"> <a href="/p/antibodies/human-mouse-hif-1-alpha-hif1a-alexa-fluor-405-conjugated-antibody-241812_ic1935v#product-documents"><span class="icon-icon-pdf text-error-red"></span> Product Documents</a> </div> </div> <div class="d-none d-md-block"> <span class="search_result_applications"></span><span class="applications_title"><strong>Applications:</strong></span> ICFlow </div> <div class="d-none d-md-block"> <span class="species_title"><strong>Reactivity:</strong></span> Human, Mouse </div> </div> <div class="col-md-3"> <button class="py-1 btn-default btn-block atc-view-more collapsed" data-toggle="collapse" href="#collapseButton-18" role="button" aria-expanded="false" aria-controls="collapseButton">View More</button> </div> </div> <div class="commerce-atc-search pt-3 collapse" id="collapseButton-18"> <div class="row"> <div class="search_result_description col-xs-12 col-sm-12"> </div> </div> <div class="row"> <div class="search_result_product_details col-xs-12 col-sm-9"> <table class="tablesaw tablesaw-stack mb-2"> <tbody> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Reactivity:</strong></td> <td class="pb-1 w-75 text-break"> Human, Mouse </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Details:</strong></td> <td class="pb-1 w-75 text-break"> Mouse IgG1 Monoclonal Clone #241812 </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Applications:</strong></td> <td class="pb-1 w-75 text-break"> ICFlow </td> </tr> </tbody> </table> <div class="compare_tool_select form-inline mb-2" data-sku="ic1935v"></div> </div> </div> <div class="atc-block-search atc-block-collapsible" data-type="search" data-blockid="ProductCart-IC1935V" > <form action="/commerce/addtocart" method="post" class="px-3 pt-3 add-to-cart-block" data-productcode="IC1935V"> <div class="commerce-atc-full-header atc-header col-four row border-bottom pb-1 m-0"> <div class="commerce-atc-catnum atc-head col-4 col-md-4 font-weight-bold">Catalog #</div> <div class="commerce-atc-availability atc-head col-md-3 font-weight-bold d-none d-md-block">Availability </div> <div class="commerce-atc-price atc-head col-5 col-md-3 font-weight-bold">Size / Price</div> <div class="commerce-atc-quantity atc-head col-3 col-md-2 text-right font-weight-bold">Qty</div> </div> <div class="spinner-border spinner-border-sm mt-3 ml-3" role="status"> <span class="sr-only">Loading...</span> </div> <div class="d-none search-product-mask"> <div id="ProductCart-IC1935V" class="atc-lines"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="IC1935V-100UG"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> IC1935V-100UG </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> </div> <div class="atc-control ProductCart-IC1935V-control text-right"> <div class="row d-flex justify-content-end mx-0 p-0"> <div class="order-md-last m___button-atc mx-1"> <button type="submit" value="Add To Cart" class="atc-add-button btn a___button-atc m-1 col-xs-4 order-last btn-md-block w-100"> Add To Cart </button> </div> <div class="m___button-atc mx-1"> <a href="/services/bulk-quotes?product_code=IC1935V" class="atc-bulk-button btn a___button-secondary m-1 col-xs-4 btn-md-block w-100" rel="nofollow">Bulk Order</a> </div> </div> </div> </div> </form> </div> </div> </div> </div> <div class="search_result atc-container pb-3" data-brand="rnd" data-category="Primary Antibodies" data-id="IC1935G" data-name="Human/Mouse HIF-1 alpha/HIF1A Alexa&nbsp;Fluor®&nbsp;488-conjugated Antibody" data-position="19"> <div class="search_details border bg-white p-3 rounded"> <div class="row"> <div class="col-md-9"> <a href="/p/antibodies/human-mouse-hif-1-alpha-hif1a-alexa-fluor-488-conjugated-antibody-241812_ic1935g" title="Human/Mouse HIF-1 alpha/HIF1A Alexa&nbsp;Fluor®&nbsp;488-conjugated Antibody"> <h4>Human/Mouse HIF-1 alpha/HIF1A Alexa Fluor® 488-conjugated Antibody </h4> </a> </div> <div class="search_result_brand_logo col-md-3 mb-1 d-flex justify-content-xl-end product-brand-logo"> <img alt="R&D Systems, a Bio-Techne brand" src="https://resources.bio-techne.com/bio-techne-assets/images/logos/bio-techne-rnd-systems.svg" class="d-flex w-75"> </div> </div> <div class="row"> <div class="col-md-9"> <div class="mb-1 d-inline-flex"> <div class="align-middle my-1 mr-3 d-block d-md-inline"> Catalog #: <a href="/p/antibodies/human-mouse-hif-1-alpha-hif1a-alexa-fluor-488-conjugated-antibody-241812_ic1935g" title="Human/Mouse HIF-1 alpha/HIF1A Alexa&nbsp;Fluor®&nbsp;488-conjugated Antibody"> IC1935G </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold"> <a href="/p/antibodies/human-mouse-hif-1-alpha-hif1a-alexa-fluor-488-conjugated-antibody-241812_ic1935g#citations"><span class="icon-icon-product-citations"></span>Citations (2)</a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold text-nowrap"> <a href="/p/antibodies/human-mouse-hif-1-alpha-hif1a-alexa-fluor-488-conjugated-antibody-241812_ic1935g#product-documents"><span class="icon-icon-pdf text-error-red"></span> Product Documents</a> </div> </div> <div class="d-none d-md-block"> <span class="search_result_applications"></span><span class="applications_title"><strong>Applications:</strong></span> ICFlow </div> <div class="d-none d-md-block"> <span class="species_title"><strong>Reactivity:</strong></span> Human, Mouse </div> </div> <div class="col-md-3"> <button class="py-1 btn-default btn-block atc-view-more collapsed" data-toggle="collapse" href="#collapseButton-19" role="button" aria-expanded="false" aria-controls="collapseButton">View More</button> </div> </div> <div class="commerce-atc-search pt-3 collapse" id="collapseButton-19"> <div class="row"> <div class="search_result_description col-xs-12 col-sm-12"> </div> </div> <div class="row"> <div class="search_result_product_details col-xs-12 col-sm-9"> <table class="tablesaw tablesaw-stack mb-2"> <tbody> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Reactivity:</strong></td> <td class="pb-1 w-75 text-break"> Human, Mouse </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Details:</strong></td> <td class="pb-1 w-75 text-break"> Mouse IgG1 Monoclonal Clone #241812 </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Applications:</strong></td> <td class="pb-1 w-75 text-break"> ICFlow </td> </tr> </tbody> </table> <div class="compare_tool_select form-inline mb-2" data-sku="ic1935g"></div> </div> </div> <div class="atc-block-search atc-block-collapsible" data-type="search" data-blockid="ProductCart-IC1935G" > <form action="/commerce/addtocart" method="post" class="px-3 pt-3 add-to-cart-block" data-productcode="IC1935G"> <div class="commerce-atc-full-header atc-header col-four row border-bottom pb-1 m-0"> <div class="commerce-atc-catnum atc-head col-4 col-md-4 font-weight-bold">Catalog #</div> <div class="commerce-atc-availability atc-head col-md-3 font-weight-bold d-none d-md-block">Availability </div> <div class="commerce-atc-price atc-head col-5 col-md-3 font-weight-bold">Size / Price</div> <div class="commerce-atc-quantity atc-head col-3 col-md-2 text-right font-weight-bold">Qty</div> </div> <div class="spinner-border spinner-border-sm mt-3 ml-3" role="status"> <span class="sr-only">Loading...</span> </div> <div class="d-none search-product-mask"> <div id="ProductCart-IC1935G" class="atc-lines"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="IC1935G-100UG"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> IC1935G-100UG </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> </div> <div class="atc-control ProductCart-IC1935G-control text-right"> <div class="row d-flex justify-content-end mx-0 p-0"> <div class="order-md-last m___button-atc mx-1"> <button type="submit" value="Add To Cart" class="atc-add-button btn a___button-atc m-1 col-xs-4 order-last btn-md-block w-100"> Add To Cart </button> </div> <div class="m___button-atc mx-1"> <a href="/services/bulk-quotes?product_code=IC1935G" class="atc-bulk-button btn a___button-secondary m-1 col-xs-4 btn-md-block w-100" rel="nofollow">Bulk Order</a> </div> </div> </div> </div> </form> </div> </div> </div> </div> <div class="search_result atc-container pb-3" data-brand="rnd" data-category="Primary Antibodies" data-id="IC1935F" data-name="Human/Mouse HIF-1 alpha/HIF1A Fluorescein-conjugated Antibody" data-position="20"> <div class="search_details border bg-white p-3 rounded"> <div class="row"> <div class="col-md-9"> <a href="/p/antibodies/human-mouse-hif-1-alpha-hif1a-fluorescein-conjugated-antibody-241812_ic1935f" title="Human/Mouse HIF-1 alpha/HIF1A Fluorescein-conjugated Antibody"> <h4>Human/Mouse HIF-1 alpha/HIF1A Fluorescein-conjugated Antibody </h4> </a> </div> <div class="search_result_brand_logo col-md-3 mb-1 d-flex justify-content-xl-end product-brand-logo"> <img alt="R&D Systems, a Bio-Techne brand" src="https://resources.bio-techne.com/bio-techne-assets/images/logos/bio-techne-rnd-systems.svg" class="d-flex w-75"> </div> </div> <div class="row"> <div class="col-md-9"> <div class="mb-1 d-inline-flex"> <div class="align-middle my-1 mr-3 d-block d-md-inline"> Catalog #: <a href="/p/antibodies/human-mouse-hif-1-alpha-hif1a-fluorescein-conjugated-antibody-241812_ic1935f" title="Human/Mouse HIF-1 alpha/HIF1A Fluorescein-conjugated Antibody"> IC1935F </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold text-nowrap"> <a href="/p/antibodies/human-mouse-hif-1-alpha-hif1a-fluorescein-conjugated-antibody-241812_ic1935f#product-documents"><span class="icon-icon-pdf text-error-red"></span> Product Documents</a> </div> <div class="d-none d-md-inline my-1"> <a href="#" class="search_result_images"><span class="icon-icon-image-camera-plus"></span> Images (1)</a> </div> </div> <div class="d-none d-md-block"> <span class="search_result_applications"></span><span class="applications_title"><strong>Applications:</strong></span> ICFlow </div> <div class="d-none d-md-block"> <span class="species_title"><strong>Reactivity:</strong></span> Human, Mouse </div> </div> <div class="col-md-3"> <button class="py-1 btn-default btn-block atc-view-more collapsed" data-toggle="collapse" href="#collapseButton-20" role="button" aria-expanded="false" aria-controls="collapseButton">View More</button> </div> </div> <div class="commerce-atc-search pt-3 collapse" id="collapseButton-20"> <div class="row"> <div class="search_result_description col-xs-12 col-md-9"> <table class="tablesaw tablesaw-stack mb-2"> <tbody> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Reactivity:</strong></td> <td class="pb-1 w-75 text-break"> Human, Mouse </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Details:</strong></td> <td class="pb-1 w-75 text-break"> Mouse IgG1 Monoclonal Clone #241812 </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Applications:</strong></td> <td class="pb-1 w-75 text-break"> ICFlow </td> </tr> </tbody> </table> <div class="compare_tool_select form-inline mb-2" data-sku="ic1935f"></div> </div> <div class="search_result_image_wrapper col-xs-12 col-md-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="rnd"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src=https://resources.bio-techne.com/images/products/antibody/HIF1_alpha_IC1935F_Flow_Cytometry_13400.jpg data-srcset=https://resources.bio-techne.com/images/products/antibody/HIF1_alpha_IC1935F_Flow_Cytometry_13400.jpg alt="Detection of HIF-1a antibody in MCF-7 Human Cell Line antibody by Flow Cytometry." title="Detection of HIF‑1 alpha in MCF‑7 Human Cell Line by Flow Cytometry." data-caption="MCF-7 human breast cancer cell line treated with CoCl<SUB>2</SUB>was stained with Mouse Anti-Human/Mouse HIF-1a Fluorescein-conjugated Monoclonal Antibody (Catalog # IC1935F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." data-badges="<span class="biological-validation"></span>" data-application="Intracellular Staining by Flow Cytometry" data-header="Human/Mouse HIF-1 alpha/HIF1A Fluorescein-conjugated Antibody, Catalog # IC1935F" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </div> </div> <div class="atc-block-search atc-block-collapsible" data-type="search" data-blockid="ProductCart-IC1935F" > <form action="/commerce/addtocart" method="post" class="px-3 pt-3 add-to-cart-block" data-productcode="IC1935F"> <div class="commerce-atc-full-header atc-header col-four row border-bottom pb-1 m-0"> <div class="commerce-atc-catnum atc-head col-4 col-md-4 font-weight-bold">Catalog #</div> <div class="commerce-atc-availability atc-head col-md-3 font-weight-bold d-none d-md-block">Availability </div> <div class="commerce-atc-price atc-head col-5 col-md-3 font-weight-bold">Size / Price</div> <div class="commerce-atc-quantity atc-head col-3 col-md-2 text-right font-weight-bold">Qty</div> </div> <div class="spinner-border spinner-border-sm mt-3 ml-3" role="status"> <span class="sr-only">Loading...</span> </div> <div class="d-none search-product-mask"> <div id="ProductCart-IC1935F" class="atc-lines"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="IC1935F"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> IC1935F </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> </div> <div class="atc-control ProductCart-IC1935F-control text-right"> <div class="row d-flex justify-content-end mx-0 p-0"> <div class="order-md-last m___button-atc mx-1"> <button type="submit" value="Add To Cart" class="atc-add-button btn a___button-atc m-1 col-xs-4 order-last btn-md-block w-100"> Add To Cart </button> </div> <div class="m___button-atc mx-1"> <a href="/services/bulk-quotes?product_code=IC1935F" class="atc-bulk-button btn a___button-secondary m-1 col-xs-4 btn-md-block w-100" rel="nofollow">Bulk Order</a> </div> </div> </div> </div> </form> </div> </div> </div> </div> <div class="search_result atc-container pb-3" data-brand="rnd" data-category="Primary Antibodies" data-id="IC1935S" data-name="Human/Mouse HIF-1 alpha/HIF1A Alexa&nbsp;Fluor®&nbsp;750-conjugated Antibody" data-position="21"> <div class="search_details border bg-white p-3 rounded"> <div class="row"> <div class="col-md-9"> <a href="/p/antibodies/human-mouse-hif-1-alpha-hif1a-alexa-fluor-750-conjugated-antibody-241812_ic1935s" title="Human/Mouse HIF-1 alpha/HIF1A Alexa&nbsp;Fluor®&nbsp;750-conjugated Antibody"> <h4>Human/Mouse HIF-1 alpha/HIF1A Alexa Fluor® 750-conjugated Antibody </h4> </a> </div> <div class="search_result_brand_logo col-md-3 mb-1 d-flex justify-content-xl-end product-brand-logo"> <img alt="R&D Systems, a Bio-Techne brand" src="https://resources.bio-techne.com/bio-techne-assets/images/logos/bio-techne-rnd-systems.svg" class="d-flex w-75"> </div> </div> <div class="row"> <div class="col-md-9"> <div class="mb-1 d-inline-flex"> <div class="align-middle my-1 mr-3 d-block d-md-inline"> Catalog #: <a href="/p/antibodies/human-mouse-hif-1-alpha-hif1a-alexa-fluor-750-conjugated-antibody-241812_ic1935s" title="Human/Mouse HIF-1 alpha/HIF1A Alexa&nbsp;Fluor®&nbsp;750-conjugated Antibody"> IC1935S </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold text-nowrap"> <a href="/p/antibodies/human-mouse-hif-1-alpha-hif1a-alexa-fluor-750-conjugated-antibody-241812_ic1935s#product-documents"><span class="icon-icon-pdf text-error-red"></span> Product Documents</a> </div> </div> <div class="d-none d-md-block"> <span class="search_result_applications"></span><span class="applications_title"><strong>Applications:</strong></span> ICFlow </div> <div class="d-none d-md-block"> <span class="species_title"><strong>Reactivity:</strong></span> Human, Mouse </div> </div> <div class="col-md-3"> <button class="py-1 btn-default btn-block atc-view-more collapsed" data-toggle="collapse" href="#collapseButton-21" role="button" aria-expanded="false" aria-controls="collapseButton">View More</button> </div> </div> <div class="commerce-atc-search pt-3 collapse" id="collapseButton-21"> <div class="row"> <div class="search_result_description col-xs-12 col-sm-12"> </div> </div> <div class="row"> <div class="search_result_product_details col-xs-12 col-sm-9"> <table class="tablesaw tablesaw-stack mb-2"> <tbody> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Reactivity:</strong></td> <td class="pb-1 w-75 text-break"> Human, Mouse </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Details:</strong></td> <td class="pb-1 w-75 text-break"> Mouse IgG1 Monoclonal Clone #241812 </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Applications:</strong></td> <td class="pb-1 w-75 text-break"> ICFlow </td> </tr> </tbody> </table> <div class="compare_tool_select form-inline mb-2" data-sku="ic1935s"></div> </div> </div> <div class="atc-block-search atc-block-collapsible" data-type="search" data-blockid="ProductCart-IC1935S" > <form action="/commerce/addtocart" method="post" class="px-3 pt-3 add-to-cart-block" data-productcode="IC1935S"> <div class="commerce-atc-full-header atc-header col-four row border-bottom pb-1 m-0"> <div class="commerce-atc-catnum atc-head col-4 col-md-4 font-weight-bold">Catalog #</div> <div class="commerce-atc-availability atc-head col-md-3 font-weight-bold d-none d-md-block">Availability </div> <div class="commerce-atc-price atc-head col-5 col-md-3 font-weight-bold">Size / Price</div> <div class="commerce-atc-quantity atc-head col-3 col-md-2 text-right font-weight-bold">Qty</div> </div> <div class="spinner-border spinner-border-sm mt-3 ml-3" role="status"> <span class="sr-only">Loading...</span> </div> <div class="d-none search-product-mask"> <div id="ProductCart-IC1935S" class="atc-lines"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="IC1935S-100UG"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> IC1935S-100UG </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> </div> <div class="atc-control ProductCart-IC1935S-control text-right"> <div class="row d-flex justify-content-end mx-0 p-0"> <div class="order-md-last m___button-atc mx-1"> <button type="submit" value="Add To Cart" class="atc-add-button btn a___button-atc m-1 col-xs-4 order-last btn-md-block w-100"> Add To Cart </button> </div> <div class="m___button-atc mx-1"> <a href="/services/bulk-quotes?product_code=IC1935S" class="atc-bulk-button btn a___button-secondary m-1 col-xs-4 btn-md-block w-100" rel="nofollow">Bulk Order</a> </div> </div> </div> </div> </form> </div> </div> </div> </div> <div class="search_result atc-container pb-3" data-brand="rnd" data-category="Primary Antibodies" data-id="IC1935U" data-name="Human/Mouse HIF-1 alpha/HIF1A Alexa&nbsp;Fluor®&nbsp;350-conjugated Antibody" data-position="22"> <div class="search_details border bg-white p-3 rounded"> <div class="row"> <div class="col-md-9"> <a href="/p/antibodies/human-mouse-hif-1-alpha-hif1a-alexa-fluor-350-conjugated-antibody-241812_ic1935u" title="Human/Mouse HIF-1 alpha/HIF1A Alexa&nbsp;Fluor®&nbsp;350-conjugated Antibody"> <h4>Human/Mouse HIF-1 alpha/HIF1A Alexa Fluor® 350-conjugated Antibody </h4> </a> </div> <div class="search_result_brand_logo col-md-3 mb-1 d-flex justify-content-xl-end product-brand-logo"> <img alt="R&D Systems, a Bio-Techne brand" src="https://resources.bio-techne.com/bio-techne-assets/images/logos/bio-techne-rnd-systems.svg" class="d-flex w-75"> </div> </div> <div class="row"> <div class="col-md-9"> <div class="mb-1 d-inline-flex"> <div class="align-middle my-1 mr-3 d-block d-md-inline"> Catalog #: <a href="/p/antibodies/human-mouse-hif-1-alpha-hif1a-alexa-fluor-350-conjugated-antibody-241812_ic1935u" title="Human/Mouse HIF-1 alpha/HIF1A Alexa&nbsp;Fluor®&nbsp;350-conjugated Antibody"> IC1935U </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold text-nowrap"> <a href="/p/antibodies/human-mouse-hif-1-alpha-hif1a-alexa-fluor-350-conjugated-antibody-241812_ic1935u#product-documents"><span class="icon-icon-pdf text-error-red"></span> Product Documents</a> </div> </div> <div class="d-none d-md-block"> <span class="search_result_applications"></span><span class="applications_title"><strong>Applications:</strong></span> ICFlow </div> <div class="d-none d-md-block"> <span class="species_title"><strong>Reactivity:</strong></span> Human, Mouse </div> </div> <div class="col-md-3"> <button class="py-1 btn-default btn-block atc-view-more collapsed" data-toggle="collapse" href="#collapseButton-22" role="button" aria-expanded="false" aria-controls="collapseButton">View More</button> </div> </div> <div class="commerce-atc-search pt-3 collapse" id="collapseButton-22"> <div class="row"> <div class="search_result_description col-xs-12 col-sm-12"> </div> </div> <div class="row"> <div class="search_result_product_details col-xs-12 col-sm-9"> <table class="tablesaw tablesaw-stack mb-2"> <tbody> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Reactivity:</strong></td> <td class="pb-1 w-75 text-break"> Human, Mouse </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Details:</strong></td> <td class="pb-1 w-75 text-break"> Mouse IgG1 Monoclonal Clone #241812 </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Applications:</strong></td> <td class="pb-1 w-75 text-break"> ICFlow </td> </tr> </tbody> </table> <div class="compare_tool_select form-inline mb-2" data-sku="ic1935u"></div> </div> </div> <div class="atc-block-search atc-block-collapsible" data-type="search" data-blockid="ProductCart-IC1935U" > <form action="/commerce/addtocart" method="post" class="px-3 pt-3 add-to-cart-block" data-productcode="IC1935U"> <div class="commerce-atc-full-header atc-header col-four row border-bottom pb-1 m-0"> <div class="commerce-atc-catnum atc-head col-4 col-md-4 font-weight-bold">Catalog #</div> <div class="commerce-atc-availability atc-head col-md-3 font-weight-bold d-none d-md-block">Availability </div> <div class="commerce-atc-price atc-head col-5 col-md-3 font-weight-bold">Size / Price</div> <div class="commerce-atc-quantity atc-head col-3 col-md-2 text-right font-weight-bold">Qty</div> </div> <div class="spinner-border spinner-border-sm mt-3 ml-3" role="status"> <span class="sr-only">Loading...</span> </div> <div class="d-none search-product-mask"> <div id="ProductCart-IC1935U" class="atc-lines"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="IC1935U-100UG"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> IC1935U-100UG </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> </div> <div class="atc-control ProductCart-IC1935U-control text-right"> <div class="row d-flex justify-content-end mx-0 p-0"> <div class="order-md-last m___button-atc mx-1"> <button type="submit" value="Add To Cart" class="atc-add-button btn a___button-atc m-1 col-xs-4 order-last btn-md-block w-100"> Add To Cart </button> </div> <div class="m___button-atc mx-1"> <a href="/services/bulk-quotes?product_code=IC1935U" class="atc-bulk-button btn a___button-secondary m-1 col-xs-4 btn-md-block w-100" rel="nofollow">Bulk Order</a> </div> </div> </div> </div> </form> </div> </div> </div> </div> <div class="search_result atc-container pb-3" data-brand="novus" data-category="Primary Antibodies" data-id="NB100-131" data-name="HIF-1 alpha Antibody (ESEE122)" data-position="23"> <div class="search_details border bg-white p-3 rounded"> <div class="row"> <div class="col-md-9"> <a href="/p/antibodies/hif-1-alpha-antibody-esee122_nb100-131" title="HIF-1 alpha Antibody (ESEE122)"> <h4>HIF-1 alpha Antibody (ESEE122) </h4> </a> </div> <div class="search_result_brand_logo col-md-3 mb-1 d-flex justify-content-xl-end product-brand-logo"> <img alt="Novus Biologicals, a Bio-Techne brand" src="https://resources.bio-techne.com/bio-techne-assets/images/logos/bio-techne-novus-biologicals.svg" class="d-flex w-75"> </div> </div> <div class="row"> <div class="col-md-9"> <div class="mb-1 d-inline-flex"> <div class="align-middle my-1 mr-3 d-block d-md-inline"> Catalog #: <a href="/p/antibodies/hif-1-alpha-antibody-esee122_nb100-131" title="HIF-1 alpha Antibody (ESEE122)"> NB100-131 </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold"> <a href="/p/antibodies/hif-1-alpha-antibody-esee122_nb100-131#reviews" class="searchImage_star4"> (3) </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold"> <a href="/p/antibodies/hif-1-alpha-antibody-esee122_nb100-131#citations"><span class="icon-icon-product-citations"></span>Citations (86)</a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold text-nowrap"> <a href="/p/antibodies/hif-1-alpha-antibody-esee122_nb100-131#product-documents"><span class="icon-icon-pdf text-error-red"></span> Product Documents</a> </div> <div class="d-none d-md-inline my-1"> <a href="#" class="search_result_images"><span class="icon-icon-image-camera-plus"></span> Images (25)</a> </div> </div> <div class="d-none d-md-block"> <span class="search_result_applications"></span><span class="applications_title"><strong>Applications:</strong></span> IHC, WB, ICC/IF, Flow, IP, Simple Western </div> <div class="d-none d-md-block"> <span class="species_title"><strong>Reactivity:</strong></span> Human, Mouse, Rat, Canine, Bovine </div> </div> <div class="col-md-3"> <button class="py-1 btn-default btn-block atc-view-more collapsed" data-toggle="collapse" href="#collapseButton-23" role="button" aria-expanded="false" aria-controls="collapseButton">View More</button> </div> </div> <div class="commerce-atc-search pt-3 collapse" id="collapseButton-23"> <div class="row"> <div class="search_result_description col-xs-12 col-md-9"> <table class="tablesaw tablesaw-stack mb-2"> <tbody> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Reactivity:</strong></td> <td class="pb-1 w-75 text-break"> Human, Mouse, Rat, Canine, Bovine </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Details:</strong></td> <td class="pb-1 w-75 text-break"> Mouse IgG1 Monoclonal Clone #ESEE122 </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Applications:</strong></td> <td class="pb-1 w-75 text-break"> IHC, WB, ICC/IF, Flow, IP, +1 More </td> </tr> </tbody> </table> <div class="compare_tool_select form-inline mb-2" data-sku="nb100-131"></div> </div> <div class="search_result_image_wrapper col-xs-12 col-md-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="novus"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src=https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-ESEE122-Immunohistochemistry-NB100-131-img0030.jpg data-srcset=https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-ESEE122-Immunohistochemistry-NB100-131-img0030.jpg alt="Immunohistochemistry: HIF-1 alpha Antibody (ESEE122) [NB100-131]" title="Immunohistochemistry: HIF-1 alpha Antibody (ESEE122) [NB100-131]" data-caption="Immunohistochemistry: HIF-1 alpha Antibody (ESEE122) [NB100-131] - Immunohistochemical analysis in non-GIST STS representing negative, and score 1-3 of Carbonic Anhydrase IX/CA9, GLUT-1, HIF-1 alpha, and HIF-2 alpha/EPAS1. non-GIST STS: non-gastrointestinal stromal tumor soft-tissue sarcomas, Carbonic Anhydrase IX/CA9: carbonic anhydrase IX, GLUT-1: glucose transporter-1, and HIF-1/2alpha: hypoxia induced factor 1/2alpha. Image collected and cropped by CiteAb from the following publication (https://www.hindawi.com/journals/sarcoma/2012/541650/), licensed under a CC-BY license." data-badges="" data-application="Immunohistochemistry" data-header="HIF-1 alpha Antibody (ESEE122), Catalog # NB100-131" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> <span class="image-count-text align-middle">(25)</span> </div> </div> </div> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-ESEE122-Immunohistochemistry-NB100-131-img0029.jpg" data-alt="Immunohistochemistry: HIF-1 alpha Antibody (ESEE122) [NB100-131]" title="Immunohistochemistry: HIF-1 alpha Antibody (ESEE122) [NB100-131]" data-caption="Immunohistochemistry: HIF-1 alpha Antibody (ESEE122) [NB100-131] - Histologically distinct cell types in hemangioblastomas do not arise from a common ancestral clone. Representative images of sample SH-0622 acquired at 400x of (a) H + E and IHC for (b) HIF1-alpha reveal heterogenous cell types in this tumor characterized by a rich vascular network. Arrowheads indicate that the stromal cells demonstrate increased cytoplasmic staining for HIF1-alpha and VEGF, whereas the double arrowheads highlight PDGFR-beta protein restricted to vascular endothelium. Scale bar is 25 um. Image collected and cropped by CiteAb from the following publication (https://www.actaneurocomms.org/content/2/1/167), licensed under a CC-BY license." data-badges="" data-application="Immunohistochemistry" data-header="HIF-1 alpha Antibody (ESEE122), Catalog # NB100-131" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-ESEE122-Simple-Western-NB100-131-img0020.jpg" data-alt="Simple Western: HIF-1 alpha Antibody (ESEE122) [NB100-131]" title="Simple Western: HIF-1 alpha Antibody (ESEE122) [NB100-131]" data-caption="Simple Western: HIF-1 alpha Antibody (ESEE122) [NB100-131] - Image shows a specific band for HIF-1 alpha in 0.5 mg/mL of Hypoxic HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system." data-badges="" data-application="Simple Western" data-header="HIF-1 alpha Antibody (ESEE122), Catalog # NB100-131" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-ESEE122-Immunocytochemistry-Immunofluorescence-NB100-131-img0017.jpg" data-alt="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody (ESEE122) [NB100-131]" title="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody (ESEE122) [NB100-131]" data-caption="Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody (ESEE122) [NB100-131] - Detection of HIF-1 alpha (red dye 568) in a cultured raw mouse macrophage cell line, using NB100-131. Photos courtesy of Susan Alexander and Hattie Gresham, PhD." data-badges="" data-application="Immunocytochemistry/ Immunofluorescence" data-header="HIF-1 alpha Antibody (ESEE122), Catalog # NB100-131" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-ESEE122-Immunocytochemistry-Immunofluorescence-NB100-131-img0016.jpg" data-alt="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody (ESEE122) [NB100-131]" title="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody (ESEE122) [NB100-131]" data-caption="Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody (ESEE122) [NB100-131] - Detection of HIF-1 alpha (red dye) in a cell cytospin from a lavage of a murine skin pouch infected with S. aureus. 100X magnification. Blue: DAPI nuclear staining." data-badges="" data-application="Immunocytochemistry/ Immunofluorescence" data-header="HIF-1 alpha Antibody (ESEE122), Catalog # NB100-131" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-ESEE122-Immunohistochemistry-Paraffin-NB100-131-img0019.jpg" data-alt="Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (ESEE122) [NB100-131]" title="Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (ESEE122) [NB100-131]" data-caption="Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (ESEE122) [NB100-131] - Analysis of a FFPE mouse kidney tissue section using HIF-1 alpha antibody clone ESEE122 at 1ug/mL concentration. The detection was performed using X-cell plus universal HRP polymer detection system with Vector SG chromagen substrate. Image courtesy of a product review by Steven Grover." data-badges="" data-application="Immunohistochemistry-Paraffin" data-header="HIF-1 alpha Antibody (ESEE122), Catalog # NB100-131" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-ESEE122-Immunohistochemistry-NB100-131-img0031.jpg" data-alt="Immunohistochemistry: HIF-1 alpha Antibody (ESEE122) [NB100-131]" title="Immunohistochemistry: HIF-1 alpha Antibody (ESEE122) [NB100-131]" data-caption="Immunohistochemistry: HIF-1 alpha Antibody (ESEE122) [NB100-131] - Immunohistochemical staining of HIF-1 alpha in normal renal tissue (A) and clear cell renal cell carcinoma (CCRCC) (D). A homogeneous cytoplasmic staining of tubular cells and weak staining in glomerules was observed with HIF-1 alpha (A). In CCRCC, HIF-1 alpha immmunoreactivity was nuclear and/or cytoplasmic (D), while it was perimembranous and/or diffuse cytoplasmic for VEGF-A and VEFG-C (E and F). (magnification x200). Image collected and cropped by CiteAb from the following publication (https://www.jeccr.com/content/28/1/40), licensed under a CC-BY license." data-badges="" data-application="Immunohistochemistry" data-header="HIF-1 alpha Antibody (ESEE122), Catalog # NB100-131" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-ESEE122-Immunohistochemistry-Paraffin-NB100-131-img0021.jpg" data-alt="Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (ESEE122) [NB100-131]" title="Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (ESEE122) [NB100-131]" data-caption="Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (ESEE122) [NB100-131] - Analysis of HIF-1 alpha in paraffin-embedded mouse kidney tissue section using anti-HIF-1 alpha antibody. Image from verified customer review." data-badges="" data-application="Immunohistochemistry-Paraffin" data-header="HIF-1 alpha Antibody (ESEE122), Catalog # NB100-131" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-ESEE122-Immunohistochemistry-NB100-131-img0033.jpg" data-alt="Immunohistochemistry: HIF-1 alpha Antibody (ESEE122) [NB100-131]" title="Immunohistochemistry: HIF-1 alpha Antibody (ESEE122) [NB100-131]" data-caption="Immunohistochemistry: HIF-1 alpha Antibody (ESEE122) [NB100-131] - Nuclear HIF-1 alpha protein expression. Xenografts were established in female nude mice (BALB c[nu/nu]) by subcutaneous injection of head and neck squamous cell carcinoma cell lines UT-SCC-2 (UT-2) and UT-SCC-14 (UT-14). Cetuximab (1 mg/injection) or PBS was administered by intraperitoneal injection at day 10, 14, and 17. A tissue microarray was constructed from tumours harvested at day 21, and the expression of nuclear HIF-1 alpha was evaluated by immunohistochemistry (IHC) in untreated controls and cetuximab-treated tumour specimens. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28756482/) licensed under a CC-BY license." data-badges="<span class="biological-validation"></span>" data-application="Immunohistochemistry" data-header="HIF-1 alpha Antibody (ESEE122), Catalog # NB100-131" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-ESEE122-Immunocytochemistry-Immunofluorescence-NB100-131-img0004.jpg" data-alt="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody (ESEE122) [NB100-131]" title="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody (ESEE122) [NB100-131]" data-caption="Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody (ESEE122) [NB100-131] - Detection of HIF-1 alpha (red dye) in a cell cytospin from a lavage of a murine skin pouch infected with S. aureus, using NB100-131. Blue: DAPI nuclear staining. Image courtesy of Susan Alexander and Hattie Gresham, PhD." data-badges="" data-application="Immunocytochemistry/ Immunofluorescence" data-header="HIF-1 alpha Antibody (ESEE122), Catalog # NB100-131" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-ESEE122-Immunocytochemistry-Immunofluorescence-NB100-131-img0007.jpg" data-alt="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody (ESEE122) [NB100-131]" title="Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody (ESEE122) [NB100-131]" data-caption="Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody (ESEE122) [NB100-131] - Detection of HIF-1 alpha (red dye 568) in a cultured raw mouse macrophage cell line. 100X magnification. Image courtesy of Susan Alexander and Hattie Gresham, PhD." data-badges="" data-application="Immunocytochemistry/ Immunofluorescence" data-header="HIF-1 alpha Antibody (ESEE122), Catalog # NB100-131" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-ESEE122-Immunohistochemistry-Paraffin-NB100-131-img0018.jpg" data-alt="Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (ESEE122) [NB100-131]" title="Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (ESEE122) [NB100-131]" data-caption="Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (ESEE122) [NB100-131] - Negative control stain of human placenta (from sea level) using mouse IgG at 1:100. 4uM paraffin-embedded section." data-badges="" data-application="Immunohistochemistry-Paraffin" data-header="HIF-1 alpha Antibody (ESEE122), Catalog # NB100-131" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-ESEE122-Immunohistochemistry-Paraffin-NB100-131-img0024.jpg" data-alt="Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (ESEE122) [NB100-131]" title="Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (ESEE122) [NB100-131]" data-caption="Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (ESEE122) [NB100-131] - Analysis of a FFPE tissue section of human renal cancer xenograft using HIF-1 alpha antibody (NB100-131 Lot 83115) at 1:200 dilution. The antibody generated a strong cytoplasmic staining mainly in the cancer cells. Only a fraction of cells depicted nuclear staining, while weak to negligible positivity was seen in the tumor stromal cells." data-badges="" data-application="Immunohistochemistry-Paraffin" data-header="HIF-1 alpha Antibody (ESEE122), Catalog # NB100-131" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-ESEE122-Immunohistochemistry-NB100-131-img0025.jpg" data-alt="Immunohistochemistry: HIF-1 alpha Antibody (ESEE122) [NB100-131]" title="Immunohistochemistry: HIF-1 alpha Antibody (ESEE122) [NB100-131]" data-caption="Immunohistochemistry: HIF-1 alpha Antibody (ESEE122) [NB100-131] - HIF-1 alpha staining in hypoxia-induced human placenta." data-badges="" data-application="Immunohistochemistry" data-header="HIF-1 alpha Antibody (ESEE122), Catalog # NB100-131" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-ESEE122-Immunohistochemistry-Paraffin-NB100-131-img0032.jpg" data-alt="Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (ESEE122) [NB100-131]" title="Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (ESEE122) [NB100-131]" data-caption="Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (ESEE122) [NB100-131] - Representative immunohistochemical expression for HIF-1alpha, c-Met, CA9 and GLUT1. HIF-1alpha is stained in cytoplasm shown with no staining in normal cervix (A), weak staining intensity in high grade CIN (B), and strong staining intensity in squamous cell carcinoma (C). Scale bar: 50 um. Image collected and cropped by CiteAb from the following publication (https://www.translational-medicine.com/content/11/1/185), licensed under a CC-BY license." data-badges="" data-application="Immunohistochemistry-Paraffin" data-header="HIF-1 alpha Antibody (ESEE122), Catalog # NB100-131" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-131_mouse-monoclonal-hif-1-alpha-antibody-esee122-271220231253347.jpg" data-alt="HIF-1 alpha Antibody (ESEE122)" title="Immunohistochemistry: HIF-1 alpha Antibody (ESEE122) [NB100-131] -" data-caption="Positive immunohistochemical staining for (A) VEGF, (B) HIF-1 alpha, (C) Dll4 (tumor cells), (D) Dll4 (endothelial cells), and (E) CD31 (for microvessel counting, ×200 magnification)." data-badges="" data-application="Immunohistochemistry" data-header="HIF-1 alpha Antibody (ESEE122), Catalog # NB100-131" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-131_mouse-monoclonal-hif-1-alpha-antibody-esee122-27122023125331.jpg" data-alt="HIF-1 alpha Antibody (ESEE122)" title="Immunohistochemistry: HIF-1 alpha Antibody (ESEE122) [NB100-131] -" data-caption="Immunohistochemical analysis in non-GIST STS representing negative, and score 1–3 of CAIX, GLUT-1, HIF-1 alpha, and HIF-2 alpha. non-GIST STS: non-gastrointestinal stromal tumor soft-tissue sarcomas, CAIX: carbonic anhydrase IX, GLUT-1: glucose transporter-1, and HIF-1/2 alpha: hypoxia induced factor 1/2 alpha." data-badges="" data-application="Immunohistochemistry" data-header="HIF-1 alpha Antibody (ESEE122), Catalog # NB100-131" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-131_mouse-monoclonal-hif-1-alpha-antibody-esee122-31020241534345.jpg" data-alt="HIF-1 alpha Antibody (ESEE122)" title="Immunohistochemistry: HIF-1 alpha Antibody (ESEE122) [NB100-131] -" data-caption="Immunohistochemistry: HIF-1 alpha Antibody (ESEE122) [NB100-131] - Histologically distinct cell types in hemangioblastomas do not arise from a common ancestral clone. “Representative images of sample SH-0622 acquired at 400x of (a) H + E & IHC for (b) HIF1-alpha, (c) VEGF, & (d) PDGFR-beta reveal heterogenous cell types in this tumor characterized by a rich vascular network. Arrowheads indicate that the stromal cells demonstrate increased cytoplasmic staining for HIF1-alpha & VEGF, whereas the double arrowheads highlight PDGFR-beta protein restricted to vascular endothelium. Scale bar is 25 μm. Image collected & cropped by CiteAb from the following publication (https://actaneurocomms.biomedcentral.com/articles/10.1186/s40478-014-0167-x), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Immunohistochemistry" data-header="HIF-1 alpha Antibody (ESEE122), Catalog # NB100-131" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-131_mouse-monoclonal-hif-1-alpha-antibody-esee122-310202415371952.jpg" data-alt="HIF-1 alpha Antibody (ESEE122)" title="Immunohistochemistry: HIF-1 alpha Antibody (ESEE122) [NB100-131] -" data-caption="Immunohistochemistry: HIF-1 alpha Antibody (ESEE122) [NB100-131] - Immunohistochemical analysis in non-GIST STS representing negative, & score 1–3 of CAIX, GLUT-1, HIF-1 alpha, & HIF-2 alpha. non-GIST STS: non-gastrointestinal stromal tumor soft-tissue sarcomas, CAIX: carbonic anhydrase IX, GLUT-1: glucose transporter-1, & HIF-1/2 alpha: hypoxia induced factor 1/2 alpha. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/22454562), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Immunohistochemistry" data-header="HIF-1 alpha Antibody (ESEE122), Catalog # NB100-131" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-131_mouse-monoclonal-hif-1-alpha-antibody-esee122-31020241534362.jpg" data-alt="HIF-1 alpha Antibody (ESEE122)" title="Immunohistochemistry: HIF-1 alpha Antibody (ESEE122) [NB100-131] -" data-caption="Immunohistochemistry: HIF-1 alpha Antibody (ESEE122) [NB100-131] - Digital image analysis of cytoplasmic & membranous staining. Cytoplasmic HIF-1 alpha staining is shown (A) & automated image analysis utilizing TissueIA recognizes cytoplasmic HIF-1 alpha staining highlighted in green color (B). CA9 is shown in membranous staining (C) & automated image analysis determines membranous CA9 staining highlighted in green color (D). The output from the algorithm returns a number of quantitative measurements for intensity & percentage of positive staining present. Scale bar: 100 μm. Image collected & cropped by CiteAb from the following publication (http://translational-medicine.biomedcentral.com/articles/10.1186/1479-5876-11-185), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Immunohistochemistry" data-header="HIF-1 alpha Antibody (ESEE122), Catalog # NB100-131" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-131_mouse-monoclonal-hif-1-alpha-antibody-esee122-310202415371953.jpg" data-alt="HIF-1 alpha Antibody (ESEE122)" title="Immunohistochemistry: HIF-1 alpha Antibody (ESEE122) [NB100-131] -" data-caption="Immunohistochemistry: HIF-1 alpha Antibody (ESEE122) [NB100-131] - Immunohistochemical staining of HIF-1 alpha, VEGF-A & VEGF-C in normal renal tissue (A-C) & clear cell renal cell carcinoma (CCRCC) (D-F). A homogeneous cytoplasmic staining of tubular cells & weak staining in glomerules was observed with HIF-1 alpha (A), while VEGF-A & VEGF-C were positive in tubular cells, glomerular mesangium & interstitial macrophages (B & C). In CCRCC, HIF-1 alpha immmunoreactivity was nuclear and/or cytoplasmic (D), while it was perimembranous and/or diffuse cytoplasmic for VEGF-A & VEFG-C (E & F). (magnification ×200). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/19302703), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Immunohistochemistry" data-header="HIF-1 alpha Antibody (ESEE122), Catalog # NB100-131" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-131_mouse-monoclonal-hif-1-alpha-antibody-esee122-310202415384140.jpg" data-alt="HIF-1 alpha Antibody (ESEE122)" title="Immunohistochemistry: HIF-1 alpha Antibody (ESEE122) [NB100-131] -" data-caption="Immunohistochemistry: HIF-1 alpha Antibody (ESEE122) [NB100-131] - Positive immunohistochemical staining for (A) VEGF, (B) HIF-1 alpha, (C) Dll4 (tumor cells), (D) Dll4 (endothelial cells), & (E) CD31 (for microvessel counting, ×200 magnification). Image collected & cropped by CiteAb from the following publication (https://www.spandidos-publications.com/10.3892/or.2012.2075), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Immunohistochemistry" data-header="HIF-1 alpha Antibody (ESEE122), Catalog # NB100-131" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-131_mouse-monoclonal-hif-1-alpha-antibody-esee122-310202415363744.jpg" data-alt="HIF-1 alpha Antibody (ESEE122)" title="Immunohistochemistry: HIF-1 alpha Antibody (ESEE122) [NB100-131] -" data-caption="Immunohistochemistry: HIF-1 alpha Antibody (ESEE122) [NB100-131] - Immunohistochemical staining of HIF-1 alpha, VEGF-A & VEGF-C in normal renal tissue (A-C) & clear cell renal cell carcinoma (CCRCC) (D-F). A homogeneous cytoplasmic staining of tubular cells & weak staining in glomerules was observed with HIF-1 alpha (A), while VEGF-A & VEGF-C were positive in tubular cells, glomerular mesangium & interstitial macrophages (B & C). In CCRCC, HIF-1 alpha immmunoreactivity was nuclear and/or cytoplasmic (D), while it was perimembranous and/or diffuse cytoplasmic for VEGF-A & VEFG-C (E & F). (magnification ×200). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/19302703), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Immunohistochemistry" data-header="HIF-1 alpha Antibody (ESEE122), Catalog # NB100-131" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-131_mouse-monoclonal-hif-1-alpha-antibody-esee122-310202415532087.jpg" data-alt="HIF-1 alpha Antibody (ESEE122)" title="Immunohistochemistry: HIF-1 alpha Antibody (ESEE122) [NB100-131] -" data-caption="Immunohistochemistry: HIF-1 alpha Antibody (ESEE122) [NB100-131] - Representative immunohistochemical expression for HIF-1 alpha, c-Met, CA9 & GLUT1. HIF-1 alpha is stained in cytoplasm shown with no staining in normal cervix (A), weak staining intensity in high grade CIN (B), & strong staining intensity in squamous cell carcinoma (C). c-Met (D-F), CA9 (G, H) & GLUT1 (I) shows cell membranous staining. Representative c-Met expression in cervical samples shown with no staining in normal cervix (D), weak membranous staining intensity in squamous cell carcinoma (E) & strong intensity in squamous cell carcinoma (F). CA9 expression showing moderate intensity staining in carcinoma in situ (CIS) (G) & strong staining in adenocarcinoma (H). GLUT1 expression showing strong intensity in squamous cell carcinoma (I). Scale bar: 50 μm. Image collected & cropped by CiteAb from the following publication (http://translational-medicine.biomedcentral.com/articles/10.1186/1479-5876-11-185), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Immunohistochemistry" data-header="HIF-1 alpha Antibody (ESEE122), Catalog # NB100-131" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nb100-131_mouse-monoclonal-hif-1-alpha-antibody-esee122-31020241553723.jpg" data-alt="HIF-1 alpha Antibody (ESEE122)" title="Immunohistochemistry: HIF-1 alpha Antibody (ESEE122) [NB100-131] -" data-caption="Immunohistochemistry: HIF-1 alpha Antibody (ESEE122) [NB100-131] - Representative immunohistochemical expression for HIF-1 alpha, c-Met, CA9 & GLUT1. HIF-1 alpha is stained in cytoplasm shown with no staining in normal cervix (A), weak staining intensity in high grade CIN (B), & strong staining intensity in squamous cell carcinoma (C). c-Met (D-F), CA9 (G, H) & GLUT1 (I) shows cell membranous staining. Representative c-Met expression in cervical samples shown with no staining in normal cervix (D), weak membranous staining intensity in squamous cell carcinoma (E) & strong intensity in squamous cell carcinoma (F). CA9 expression showing moderate intensity staining in carcinoma in situ (CIS) (G) & strong staining in adenocarcinoma (H). GLUT1 expression showing strong intensity in squamous cell carcinoma (I). Scale bar: 50 μm. Image collected & cropped by CiteAb from the following publication (http://translational-medicine.biomedcentral.com/articles/10.1186/1479-5876-11-185), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Immunohistochemistry" data-header="HIF-1 alpha Antibody (ESEE122), Catalog # NB100-131" data-width="250" data-height="250" class="none"></i> </div> </div> </div> </div> <div class="atc-block-search atc-block-collapsible" data-type="search" data-blockid="ProductCart-NB100-131" > <form action="/commerce/addtocart" method="post" class="px-3 pt-3 add-to-cart-block" data-productcode="NB100-131"> <div class="commerce-atc-full-header atc-header col-four row border-bottom pb-1 m-0"> <div class="commerce-atc-catnum atc-head col-4 col-md-4 font-weight-bold">Catalog #</div> <div class="commerce-atc-availability atc-head col-md-3 font-weight-bold d-none d-md-block">Availability </div> <div class="commerce-atc-price atc-head col-5 col-md-3 font-weight-bold">Size / Price</div> <div class="commerce-atc-quantity atc-head col-3 col-md-2 text-right font-weight-bold">Qty</div> </div> <div class="spinner-border spinner-border-sm 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<h4>HIF-1 alpha Antibody Pack </h4> </a> </div> <div class="search_result_brand_logo col-md-3 mb-1 d-flex justify-content-xl-end product-brand-logo"> <img alt="Novus Biologicals, a Bio-Techne brand" src="https://resources.bio-techne.com/bio-techne-assets/images/logos/bio-techne-novus-biologicals.svg" class="d-flex w-75"> </div> </div> <div class="row"> <div class="col-md-9"> <div class="mb-1 d-inline-flex"> <div class="align-middle my-1 mr-3 d-block d-md-inline"> Catalog #: <a href="/p/antibodies/hif-1-alpha-antibody-pack_nb100-900wb" title="HIF-1 alpha Antibody Pack"> NB100-900WB </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold"> <a href="/p/antibodies/hif-1-alpha-antibody-pack_nb100-900wb#citations"><span class="icon-icon-product-citations"></span>Citations (42)</a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold text-nowrap"> <a href="/p/antibodies/hif-1-alpha-antibody-pack_nb100-900wb#product-documents"><span class="icon-icon-pdf text-error-red"></span> Product Documents</a> </div> <div class="d-none d-md-inline my-1"> <a href="#" class="search_result_images"><span class="icon-icon-image-camera-plus"></span> Images (9)</a> </div> </div> <div class="d-none d-md-block"> <span class="search_result_applications"></span><span class="applications_title"><strong>Applications:</strong></span> IHC, WB, ICC/IF, IP, Simple Western, ChIP </div> <div class="d-none d-md-block"> <span class="species_title"><strong>Reactivity:</strong></span> Human, Mouse, Rat, Canine, Primate, +1 More </div> </div> <div class="col-md-3"> <button class="py-1 btn-default btn-block atc-view-more collapsed" data-toggle="collapse" href="#collapseButton-24" role="button" aria-expanded="false" aria-controls="collapseButton">View More</button> </div> </div> <div class="commerce-atc-search pt-3 collapse" id="collapseButton-24"> <div class="row"> <div class="search_result_description col-xs-12 col-md-9"> <table class="tablesaw tablesaw-stack mb-2"> <tbody> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Applications:</strong></td> <td class="pb-1 w-75 text-break"> IHC, WB, ICC/IF, IP, Simple Western, +1 More </td> </tr> </tbody> </table> <div class="compare_tool_select form-inline mb-2" data-sku="nb100-900wb"></div> </div> <div class="search_result_image_wrapper col-xs-12 col-md-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="novus"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src=https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-Pack-Western-Blot-NB100-900WB-img0019.jpg data-srcset=https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-Pack-Western-Blot-NB100-900WB-img0019.jpg alt="Western Blot: HIF-1 alpha Antibody Pack [NB100-900WB]" title="Western Blot: HIF-1 alpha Antibody Pack [NB100-900WB]" data-caption="Western Blot: HIF-1 alpha Antibody Pack [NB100-900WB] - HIF-1 alpha induction by CoCl2 on Caki-1 cell lysate. HIF-1 alpha antibody (H1alpha67) [NB100-105]. Western blot image submitted by a verified customer review." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody Pack, Catalog # NB100-900WB" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> <span class="image-count-text align-middle">(9)</span> </div> </div> </div> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-Pack-Simple-Western-NB100-900WB-img0018.jpg" data-alt="Simple Western: HIF-1 alpha Antibody Pack [NB100-900WB]" title="Simple Western: HIF-1 alpha Antibody Pack [NB100-900WB]" data-caption="Simple Western: HIF-1 alpha Antibody Pack [NB100-900WB] - Simple Western lane view shows a specific band for HIF-1 alpha in 0.5 mg/mL of hypoxic HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. HIF-1 alpha Antibody [NB100-449]." data-badges="" data-application="Simple Western" data-header="HIF-1 alpha Antibody Pack, Catalog # NB100-900WB" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-Pack-Simple-Western-NB100-900WB-img0022.jpg" data-alt="Simple Western: HIF-1 alpha Antibody Pack [NB100-900WB]" title="Simple Western: HIF-1 alpha Antibody Pack [NB100-900WB]" data-caption="Simple Western: HIF-1 alpha Antibody Pack [NB100-900WB] - Image shows a specific band for HIF-1 alpha in 0.2 mg/mL of Hypoxic HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system." data-badges="" data-application="Simple Western" data-header="HIF-1 alpha Antibody Pack, Catalog # NB100-900WB" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-Pack-Knockout-Validated-NB100-900WB-img0020.jpg" data-alt="Knockout Validated: HIF-1 alpha Antibody Pack [NB100-900WB]" title="Knockout Validated: HIF-1 alpha Antibody Pack [NB100-900WB]" data-caption="Knockout Validated: HIF-1 alpha Antibody Pack [NB100-900WB] - Naive CD4 T cells from WT, VHL-deficient (Vhl KO), or HIF-1 alpha-deficient (HIF-1 alpha KO) mice were differentiated under IL-22-skewing conditions for a total of 60 h. Some cells remained at normoxia for the duration of the culture (N); others were at normoxia for 35 h and then hypoxia (1% O2) for 24 h (H). At 60 h, nuclear extracts were harvested, and HIF-1 alpha and Lamin B1 levels were analyzed by Western blot. Image from verified customer review. HIF-1 alpha Antibody (H1alpha67) [NB100-105]" data-badges="" data-application="Knockout Validated" data-header="HIF-1 alpha Antibody Pack, Catalog # NB100-900WB" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-Pack-Western-Blot-NB100-900WB-img0015.jpg" data-alt="Western Blot: HIF-1 alpha Antibody Pack [NB100-900WB]" title="Western Blot: HIF-1 alpha Antibody Pack [NB100-900WB]" data-caption="Western Blot: HIF-1 alpha Antibody Pack [NB100-900WB] - Normoxic pre-treatment sensitizes glioma cells to radiation after graded chronic hypoxic (GCH) exposure. The results of anchorage-independent colony forming assays are shown for U87, U87-luc, GL261 glioma cells and 0308 GSCs after 5 Gy radiation exposure under varying O2 conditions. For easier comparisons among cell types, raw values are expressed as a % of the cell type's negative (non-irradiated) control and the means & SEMs are plotted. Also shown are Western blots of nuclear HIF-1alpha at the time of irradiation. Corresponding Western blots of lamin A/C are shown as a loading control. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0111199) licensed under a CC-BY license. HIF-1 alpha Antibody [NB100-479]" data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody Pack, Catalog # NB100-900WB" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-Pack-Knockdown-Validated-NB100-900WB-img0017.jpg" data-alt="Knockdown Validated: HIF-1 alpha Antibody Pack [NB100-900WB]" title="Knockdown Validated: HIF-1 alpha Antibody Pack [NB100-900WB]" data-caption="Knockdown Validated: HIF-1 alpha Antibody Pack [NB100-900WB] - Morphological alterations induced by DMOG are HIF-1alpha dependent. glEND.2 cells were stably transfected with shGFP, shHIF-1alpha or shHIF-2alpha. The clones were cultured under normoxic (N) or hypoxic (H, 1% O2) conditions, or treated with DMOG for 6 h. 30 ug protein of cellular protein were loaded for the detection of HIF-1alpha, and nuclear extracts (20 ug protein) were used for the detection of HIF-2alpha. Image collected and cropped by CiteAb from the following publication (//biosignaling.biomedcentral.com/articles/10.1186/1478-811X-11-80) licensed under a CC-BY license. HIF-1 alpha Antibody [NB100-449]" data-badges="" data-application="Knockdown Validated" data-header="HIF-1 alpha Antibody Pack, Catalog # NB100-900WB" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-Pack-Western-Blot-NB100-900WB-img0021.jpg" data-alt="Western Blot: HIF-1 alpha Antibody Pack [NB100-900WB]" title="Western Blot: HIF-1 alpha Antibody Pack [NB100-900WB]" data-caption="Western Blot: HIF-1 alpha Antibody Pack [NB100-900WB] - Analysis of HIF-1 alpha in overexpression and endogenous HIF-1 alpha & HIF-2 alpha using anti-HIF-1 alpha antibody NB100-134. The data showed that HIF-1 alpha antibody did not react to HIF-2 alpha overexpression. Wester blot image submitted by a verified customer review." data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody Pack, Catalog # NB100-900WB" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-Pack-Western-Blot-NB100-900WB-img0016.jpg" data-alt="Western Blot: HIF-1 alpha Antibody Pack [NB100-900WB]" title="Western Blot: HIF-1 alpha Antibody Pack [NB100-900WB]" data-caption="Western Blot: HIF-1 alpha Antibody Pack [NB100-900WB] - HIF-1 alpha induction on Caki-1 cell lysate using CoCl2. Western blot image submitted by a verified customer review. HIF-1 alpha Antibody [NB100-449]" data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody Pack, Catalog # NB100-900WB" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-Pack-Western-Blot-NB100-900WB-img0023.jpg" data-alt="Western Blot: HIF-1 alpha Antibody Pack [NB100-900WB]" title="Western Blot: HIF-1 alpha Antibody Pack [NB100-900WB]" data-caption="Western Blot: HIF-1 alpha Antibody Pack [NB100-900WB] - Detection of HIF-1 alpha (125-130 kDa) from human placental villous explant total protein. HIF-1 alpha Antibody (HA111) [NB100-296]" data-badges="" data-application="Western Blot" data-header="HIF-1 alpha Antibody Pack, Catalog # NB100-900WB" data-width="250" data-height="250" class="none"></i> </div> </div> </div> </div> <div class="atc-block-search atc-block-collapsible" data-type="search" data-blockid="ProductCart-NB100-900WB" > <form action="/commerce/addtocart" method="post" class="px-3 pt-3 add-to-cart-block" data-productcode="NB100-900WB"> <div class="commerce-atc-full-header atc-header col-four row border-bottom pb-1 m-0"> <div class="commerce-atc-catnum atc-head col-4 col-md-4 font-weight-bold">Catalog #</div> <div class="commerce-atc-availability atc-head col-md-3 font-weight-bold d-none d-md-block">Availability </div> <div class="commerce-atc-price atc-head col-5 col-md-3 font-weight-bold">Size / Price</div> <div class="commerce-atc-quantity atc-head col-3 col-md-2 text-right font-weight-bold">Qty</div> </div> <div class="spinner-border spinner-border-sm mt-3 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HIF-1 alpha/HIF1A: Antibodies