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<!DOCTYPE html> <html > <head> <meta charset="utf-8"> <meta rel="search" type="application/opensearchdescription+xml" href="/open_search.xml" title="Academia.edu"> <meta content="width=device-width, initial-scale=1" name="viewport"> <meta name="google-site-verification" content="bKJMBZA7E43xhDOopFZkssMMkBRjvYERV-NaN4R6mrs"> <meta name="csrf-param" content="authenticity_token" /> <meta name="csrf-token" content="CXByLVrUaidjGSNc37XwYSSLcNWfQAwX51r7RKKElkb3YfkpnAPtdA+zXJsIq0D0aAFtjUjgUUJBWPl5KFqLgw==" /> <meta name="citation_title" content="Isolation and characterization of vB_XciM_LucasX, a new jumbo phage that infects Xanthomonas citri and Xanthomonas fuscans" /> <meta name="citation_journal_title" content="PLOS ONE" /> <meta name="citation_author" content="Franklin Behlau" /> <meta name="twitter:card" content="summary" /> <meta name="twitter:url" content="https://www.academia.edu/86921238/Isolation_and_characterization_of_vB_XciM_LucasX_a_new_jumbo_phage_that_infects_Xanthomonas_citri_and_Xanthomonas_fuscans" /> <meta name="twitter:title" content="Isolation and characterization of vB_XciM_LucasX, a new jumbo phage that infects Xanthomonas citri and Xanthomonas fuscans" /> <meta name="twitter:description" content="Citrus canker is one of the main bacterial diseases that affect citrus crops and is caused by Xanthomonas citri which affects all citrus species worldwide. New strategies to control citrus canker are necessary and the use of bacteriophages as" /> <meta name="twitter:image" content="http://a.academia-assets.com/images/twitter-card.jpeg" /> <meta property="fb:app_id" content="2369844204" /> <meta property="og:type" content="article" /> <meta property="og:url" content="https://www.academia.edu/86921238/Isolation_and_characterization_of_vB_XciM_LucasX_a_new_jumbo_phage_that_infects_Xanthomonas_citri_and_Xanthomonas_fuscans" /> <meta property="og:title" content="Isolation and characterization of vB_XciM_LucasX, a new jumbo phage that infects Xanthomonas citri and Xanthomonas fuscans" /> <meta property="og:image" content="http://a.academia-assets.com/images/open-graph-icons/fb-paper.gif" /> <meta property="og:description" content="Citrus canker is one of the main bacterial diseases that affect citrus crops and is caused by Xanthomonas citri which affects all citrus species worldwide. New strategies to control citrus canker are necessary and the use of bacteriophages as" /> <meta property="article:author" content="https://independent.academia.edu/FranklinBehlau" /> <meta name="description" content="Citrus canker is one of the main bacterial diseases that affect citrus crops and is caused by Xanthomonas citri which affects all citrus species worldwide. New strategies to control citrus canker are necessary and the use of bacteriophages as" /> <title>(PDF) Characterization of jumbo phage vB_XciM_LucasX infecting citrus bacteria</title> <link rel="canonical" href="https://www.academia.edu/86921238/Isolation_and_characterization_of_vB_XciM_LucasX_a_new_jumbo_phage_that_infects_Xanthomonas_citri_and_Xanthomonas_fuscans" /> <script async src="https://www.googletagmanager.com/gtag/js?id=G-5VKX33P2DS"></script> <script> window.dataLayer = window.dataLayer || []; function gtag(){dataLayer.push(arguments);} gtag('js', new Date()); gtag('config', 'G-5VKX33P2DS', { cookie_domain: 'academia.edu', send_page_view: false, }); gtag('event', 'page_view', { 'controller': "single_work", 'action': "show", 'controller_action': 'single_work#show', 'logged_in': 'false', 'edge': 'unknown', // Send nil if there is no A/B test bucket, in case some records get logged // with missing data - that way we can distinguish between the two cases. // ab_test_bucket should be of the form <ab_test_name>:<bucket> 'ab_test_bucket': null, }) </script> <script> var $controller_name = 'single_work'; var $action_name = "show"; var $rails_env = 'production'; var $app_rev = '48654d67e5106e06fb1e5c9a356c302510d6cfee'; var $domain = 'academia.edu'; var $app_host = "academia.edu"; var $asset_host = "academia-assets.com"; var $start_time = new Date().getTime(); var $recaptcha_key = "6LdxlRMTAAAAADnu_zyLhLg0YF9uACwz78shpjJB"; var $recaptcha_invisible_key = "6Lf3KHUUAAAAACggoMpmGJdQDtiyrjVlvGJ6BbAj"; var $disableClientRecordHit = false; </script> <script> window.require = { config: function() { return function() {} } } </script> <script> window.Aedu = window.Aedu || {}; window.Aedu.hit_data = null; window.Aedu.serverRenderTime = new Date(1734497125000); window.Aedu.timeDifference = new Date().getTime() - 1734497125000; </script> <script type="application/ld+json">{"@context":"https://schema.org","@type":"ScholarlyArticle","abstract":"Citrus canker is one of the main bacterial diseases that affect citrus crops and is caused by Xanthomonas citri which affects all citrus species worldwide. New strategies to control citrus canker are necessary and the use of bacteriophages as biocontrol agent could be an alternative. Phages that infect Xanthomonas species have been studied, such as XacN1, a myovirus that infects X. citri. Here we report the isolation and characterization of a new jumbo phage, vb_XciM_LucasX, which infects X. citri and X. fuscans. Transmission electron microscopy allowed classification of LucasX in the Myoviridae family, which was corroborated by its genomic sequencing, annotation, and proteome clustering. LucasX has a 305,651 bp-long dsDNA genome. ORF prediction and annotation revealed 157 genes encoding putative structural proteins such as capsid and tail related proteins and phage assembly associated proteins, however, for most of the structural proteins it was not possible assign specific functio...","author":[{"@context":"https://schema.org","@type":"Person","name":"Franklin Behlau"}],"contributor":[],"dateCreated":"2022-09-19","dateModified":"2024-11-28","headline":"Isolation and characterization of vB_XciM_LucasX, a new jumbo phage that infects Xanthomonas citri and Xanthomonas fuscans","image":"https://attachments.academia-assets.com/91268320/thumbnails/1.jpg","inLanguage":"en","keywords":["Biology","Multidisciplinary","Genome","Capsid","PLoS one","Citrus Canker","Xanthomonas","Proteome","Xanthomonas citri"],"publication":"PLOS ONE","publisher":{"@context":"https://schema.org","@type":"Organization","name":"Public Library of Science (PLoS)"},"sourceOrganization":[{"@context":"https://schema.org","@type":"EducationalOrganization","name":null}],"thumbnailUrl":"https://attachments.academia-assets.com/91268320/thumbnails/1.jpg","url":"https://www.academia.edu/86921238/Isolation_and_characterization_of_vB_XciM_LucasX_a_new_jumbo_phage_that_infects_Xanthomonas_citri_and_Xanthomonas_fuscans"}</script><link rel="stylesheet" media="all" href="//a.academia-assets.com/assets/single_work_page/loswp-102fa537001ba4d8dcd921ad9bd56c474abc201906ea4843e7e7efe9dfbf561d.css" /><link rel="stylesheet" media="all" href="//a.academia-assets.com/assets/design_system/body-8d679e925718b5e8e4b18e9a4fab37f7eaa99e43386459376559080ac8f2856a.css" /><link rel="stylesheet" media="all" href="//a.academia-assets.com/assets/design_system/button-3cea6e0ad4715ed965c49bfb15dedfc632787b32ff6d8c3a474182b231146ab7.css" /><link rel="stylesheet" media="all" 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New strategies to control citrus canker are necessary and the use of bacteriophages as biocontrol agent could be an alternative. Phages that infect Xanthomonas species have been studied, such as XacN1, a myovirus that infects X. citri. Here we report the isolation and characterization of a new jumbo phage, vb_XciM_LucasX, which infects X. citri and X. fuscans. Transmission electron microscopy allowed classification of LucasX in the Myoviridae family, which was corroborated by its genomic sequencing, annotation, and proteome clustering. LucasX has a 305,651 bp-long dsDNA genome. 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window.loginModal = {}; window.loginModal.appleClientId = 'edu.academia.applesignon';</script><script defer="" src="https://accounts.google.com/gsi/client"></script><div class="ds-loswp-container"><div class="ds-work-card--grid-container"><div class="ds-work-card--container js-loswp-work-card"><div class="ds-work-card--cover"><div class="ds-work-cover--wrapper"><div class="ds-work-cover--container"><button class="ds-work-cover--clickable js-swp-download-button" data-signup-modal="{"location":"swp-splash-paper-cover","attachmentId":91268320,"attachmentType":"pdf"}"><img alt="First page of “Isolation and characterization of vB_XciM_LucasX, a new jumbo phage that infects Xanthomonas citri and Xanthomonas fuscans”" class="ds-work-cover--cover-thumbnail" src="https://0.academia-photos.com/attachment_thumbnails/91268320/mini_magick20220919-1-19eh0dl.png?1663613015" /><img alt="PDF Icon" class="ds-work-cover--file-icon" src="//a.academia-assets.com/images/single_work_splash/adobe_icon.svg" /><div class="ds-work-cover--hover-container"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">download</span><p>Download Free PDF</p></div><div class="ds-work-cover--ribbon-container">Download Free PDF</div><div class="ds-work-cover--ribbon-triangle"></div></button></div></div></div><div class="ds-work-card--work-information"><h1 class="ds-work-card--work-title">Isolation and characterization of vB_XciM_LucasX, a new jumbo phage that infects Xanthomonas citri and Xanthomonas fuscans</h1><div class="ds-work-card--work-authors ds-work-card--detail"><a class="ds-work-card--author js-wsj-grid-card-author ds2-5-body-md ds2-5-body-link" data-author-id="64890966" href="https://independent.academia.edu/FranklinBehlau"><img alt="Profile image of Franklin Behlau" class="ds-work-card--author-avatar" src="//a.academia-assets.com/images/s65_no_pic.png" />Franklin Behlau</a></div><div class="ds-work-card--detail"><p class="ds-work-card--detail ds2-5-body-sm">PLOS ONE</p><div class="ds-work-card--work-metadata"><div class="ds-work-card--work-metadata__stat"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">visibility</span><p class="ds2-5-body-sm" id="work-metadata-view-count">…</p></div><div class="ds-work-card--work-metadata__stat"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">description</span><p class="ds2-5-body-sm">19 pages</p></div><div class="ds-work-card--work-metadata__stat"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">link</span><p class="ds2-5-body-sm">1 file</p></div></div><script>(async () => { const workId = 86921238; 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if (!viewCountBody) { throw new Error('Failed to find work views element'); } viewCountBody.textContent = `${commaizedViewCount} views`; } catch (error) { // Remove the whole views element if there was some issue parsing. document.getElementById('work-metadata-view-count')?.parentNode?.remove(); throw new Error(`Failed to parse view count: ${viewCount}`, error); } }; // If the DOM is still loading, wait for it to be ready before updating the view count. if (document.readyState === "loading") { document.addEventListener('DOMContentLoaded', () => { updateViewCount(viewCount); }); // Otherwise, just update it immediately. } else { updateViewCount(viewCount); } })();</script></div><p class="ds-work-card--work-abstract ds-work-card--detail ds2-5-body-md">Citrus canker is one of the main bacterial diseases that affect citrus crops and is caused by Xanthomonas citri which affects all citrus species worldwide. New strategies to control citrus canker are necessary and the use of bacteriophages as biocontrol agent could be an alternative. Phages that infect Xanthomonas species have been studied, such as XacN1, a myovirus that infects X. citri. Here we report the isolation and characterization of a new jumbo phage, vb_XciM_LucasX, which infects X. citri and X. fuscans. Transmission electron microscopy allowed classification of LucasX in the Myoviridae family, which was corroborated by its genomic sequencing, annotation, and proteome clustering. LucasX has a 305,651 bp-long dsDNA genome. ORF prediction and annotation revealed 157 genes encoding putative structural proteins such as capsid and tail related proteins and phage assembly associated proteins, however, for most of the structural proteins it was not possible assign specific functio...</p><div class="ds-work-card--button-container"><button class="ds2-5-button js-swp-download-button" data-signup-modal="{"location":"continue-reading-button--work-card","attachmentId":91268320,"attachmentType":"pdf","workUrl":"https://www.academia.edu/86921238/Isolation_and_characterization_of_vB_XciM_LucasX_a_new_jumbo_phage_that_infects_Xanthomonas_citri_and_Xanthomonas_fuscans"}">See full PDF</button><button class="ds2-5-button ds2-5-button--secondary js-swp-download-button" data-signup-modal="{"location":"download-pdf-button--work-card","attachmentId":91268320,"attachmentType":"pdf","workUrl":"https://www.academia.edu/86921238/Isolation_and_characterization_of_vB_XciM_LucasX_a_new_jumbo_phage_that_infects_Xanthomonas_citri_and_Xanthomonas_fuscans"}"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">download</span>Download PDF</button></div></div></div></div><div data-auto_select="false" data-client_id="331998490334-rsn3chp12mbkiqhl6e7lu2q0mlbu0f1b" data-doc_id="91268320" data-landing_url="https://www.academia.edu/86921238/Isolation_and_characterization_of_vB_XciM_LucasX_a_new_jumbo_phage_that_infects_Xanthomonas_citri_and_Xanthomonas_fuscans" data-login_uri="https://www.academia.edu/registrations/google_one_tap" data-moment_callback="onGoogleOneTapEvent" id="g_id_onload"></div><div class="ds-top-related-works--grid-container"><div class="ds-related-content--container ds-top-related-works--container"><h2 class="ds-related-content--heading">Related papers</h2><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="0" data-entity-id="31533246" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/31533246/The_filamentous_phage_XacF1_causes_loss_of_virulence_in_Xanthomonas_axonopodis_pv_citri_the_causative_agent_of_citrus_canker_disease">The filamentous phage XacF1 causes loss of virulence in Xanthomonas axonopodis pv. citri, the causative agent of citrus canker disease</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="33025625" href="https://zagazig.academia.edu/ahmedaskora">ahmed askora</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Frontiers in microbiology, 2014</p><p class="ds-related-work--abstract ds2-5-body-sm">In this study, filamentous phage XacF1, which can infect Xanthomonas axonopodis pv. citri (Xac) strains, was isolated and characterized. Electron microscopy showed that XacF1 is a member of the family Inoviridae and is about 600 nm long. The genome of XacF1 is 7325 nucleotides in size, containing 13 predicted open reading frames (ORFs), some of which showed significant homology to Ff-like phage proteins such as ORF1 (pII), ORF2 (pV), ORF6 (pIII), and ORF8 (pVI). XacF1 showed a relatively wide host range, infecting seven out of 11 strains tested in this study. Frequently, XacF1 was found to be integrated into the genome of Xac strains. This integration occurred at the host dif site (attB) and was mediated by the host XerC/D recombination system. The attP sequence was identical to that of Xanthomonas phage Cf1c. Interestingly, infection by XacF1 phage caused several physiological changes to the bacterial host cells, including lower levels of extracellular polysaccharide production, re...</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"The filamentous phage XacF1 causes loss of virulence in Xanthomonas axonopodis pv. citri, the causative agent of citrus canker disease","attachmentId":51875114,"attachmentType":"pdf","work_url":"https://www.academia.edu/31533246/The_filamentous_phage_XacF1_causes_loss_of_virulence_in_Xanthomonas_axonopodis_pv_citri_the_causative_agent_of_citrus_canker_disease","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/31533246/The_filamentous_phage_XacF1_causes_loss_of_virulence_in_Xanthomonas_axonopodis_pv_citri_the_causative_agent_of_citrus_canker_disease"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="1" data-entity-id="115576110" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/115576110/Dynamic_integration_and_excision_of_filamentous_phage_XacF1_inXanthomonas_citripv_citri_the_causative_agent_of_citrus_canker_disease">Dynamic integration and excision of filamentous phage XacF1 inXanthomonas citripv.citri, the causative agent of citrus canker disease</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="33025625" href="https://zagazig.academia.edu/ahmedaskora">ahmed askora</a></div><p class="ds-related-work--metadata ds2-5-body-xs">FEBS Open Bio, 2017</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Dynamic integration and excision of filamentous phage XacF1 inXanthomonas citripv.citri, the causative agent of citrus canker disease","attachmentId":111943476,"attachmentType":"pdf","work_url":"https://www.academia.edu/115576110/Dynamic_integration_and_excision_of_filamentous_phage_XacF1_inXanthomonas_citripv_citri_the_causative_agent_of_citrus_canker_disease","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/115576110/Dynamic_integration_and_excision_of_filamentous_phage_XacF1_inXanthomonas_citripv_citri_the_causative_agent_of_citrus_canker_disease"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="2" data-entity-id="72256051" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/72256051/Xanthomonas_citri_jumbo_phage_XacN1_exhibits_a_wide_host_range_and_high_complement_of_tRNA_genes">Xanthomonas citri jumbo phage XacN1 exhibits a wide host range and high complement of tRNA genes</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="33025625" href="https://zagazig.academia.edu/ahmedaskora">ahmed askora</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Scientific reports, 2018</p><p class="ds-related-work--abstract ds2-5-body-sm">Xanthomonas virus (phage) XacN1 is a novel jumbo myovirus infecting Xanthomonas citri, the causative agent of Asian citrus canker. Its linear 384,670 bp double-stranded DNA genome encodes 592 proteins and presents the longest (66 kbp) direct terminal repeats (DTRs) among sequenced viral genomes. The DTRs harbor 56 tRNA genes, which correspond to all 20 amino acids and represent the largest number of tRNA genes reported in a viral genome. Codon usage analysis revealed a propensity for the phage encoded tRNAs to target codons that are highly used by the phage but less frequently by its host. The existence of these tRNA genes and seven additional translation-related genes as well as a chaperonin gene found in the XacN1 genome suggests a relative independence of phage replication on host molecular machinery, leading to a prediction of a wide host range for this jumbo phage. We confirmed the prediction by showing a wider host range of XacN1 than other X. citri phages in an infection test...</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Xanthomonas citri jumbo phage XacN1 exhibits a wide host range and high complement of tRNA genes","attachmentId":81255993,"attachmentType":"pdf","work_url":"https://www.academia.edu/72256051/Xanthomonas_citri_jumbo_phage_XacN1_exhibits_a_wide_host_range_and_high_complement_of_tRNA_genes","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/72256051/Xanthomonas_citri_jumbo_phage_XacN1_exhibits_a_wide_host_range_and_high_complement_of_tRNA_genes"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="3" data-entity-id="41500642" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/41500642/Characterization_of_two_related_Erwinia_myoviruses_that_are_distant_relatives_of_the_PhiKZ_like_Jumbo_phages">Characterization of two related Erwinia myoviruses that are distant relatives of the PhiKZ-like Jumbo phages</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="20669274" href="https://byu.academia.edu/JasonStettler">Jason Stettler</a></div><p class="ds-related-work--metadata ds2-5-body-xs">2018</p><p class="ds-related-work--abstract ds2-5-body-sm">Bacteriophages are a major force in the evolution of bacteria due to their sheer abundance as well as their ability to infect and kill their hosts and to transfer genetic material. Bacterio-phages that infect the Enterobacteriaceae family are of particular interest because this bacterial family contains dangerous animal and plant pathogens. Herein we report the isolation and characterization of two jumbo myovirus Erwinia phages, RisingSun and Joad, collected from apple trees. These two genomes are nearly identical with Joad harboring two additional putative gene products. Despite mass spectrometry data that support the putative annotation , 43% of their gene products have no significant BLASTP hit. These phages are also more closely related to Pseudomonas and Vibrio phages than to published Enterobacteria-ceae phages. Of the 140 gene products with a BLASTP hit, 81% and 63% of the closest hits correspond to gene products from Pseudomonas and Vibrio phages, respectively. This relatedness may reflect their ecological niche, rather than the evolutionary history of their host. Despite the presence of over 800 Enterobacteriaceae phages on NCBI, the uniqueness of these two phages highlights the diversity of Enterobacteriaceae phages still to be discovered.</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Characterization of two related Erwinia myoviruses that are distant relatives of the PhiKZ-like Jumbo phages","attachmentId":61671108,"attachmentType":"pdf","work_url":"https://www.academia.edu/41500642/Characterization_of_two_related_Erwinia_myoviruses_that_are_distant_relatives_of_the_PhiKZ_like_Jumbo_phages","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/41500642/Characterization_of_two_related_Erwinia_myoviruses_that_are_distant_relatives_of_the_PhiKZ_like_Jumbo_phages"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="4" data-entity-id="70969129" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/70969129/Genomic_analysis_of_bacteriophage_Xoo_sp13_infecting_Xanthomonas_oryzae_pv_oryzae">Genomic analysis of bacteriophage Xoo-sp13 infecting Xanthomonas oryzae pv. oryzae</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="37512161" href="https://independent.academia.edu/rasheedmadiha">madiha rasheed</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Archives of Virology</p><p class="ds-related-work--abstract ds2-5-body-sm">Xanthomonas oryzae pv. oryzae is a bacterial pathogen that gives rise to diseases in rice all over the world. A bacteriophage infecting this bacterium was isolated from rice fields in China. Here, we report the complete genome sequence of this phage, which has a linear dsDNA genome of 309,023 bp and a G + C content of 42.43%. It contains 401 open reading frames and encodes 28 tRNAs. It belongs to the family Myoviridae and has a broad host range, making it a possible candidate for phage therapy.</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Genomic analysis of bacteriophage Xoo-sp13 infecting Xanthomonas oryzae pv. oryzae","attachmentId":80501802,"attachmentType":"pdf","work_url":"https://www.academia.edu/70969129/Genomic_analysis_of_bacteriophage_Xoo_sp13_infecting_Xanthomonas_oryzae_pv_oryzae","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/70969129/Genomic_analysis_of_bacteriophage_Xoo_sp13_infecting_Xanthomonas_oryzae_pv_oryzae"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="5" data-entity-id="84078887" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/84078887/Isolation_of_Novel_Xanthomonas_Phages_Infecting_the_Plant_Pathogens_X_translucens_and_X_campestris">Isolation of Novel Xanthomonas Phages Infecting the Plant Pathogens X. translucens and X. campestris</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="97227300" href="https://nationalagriculturalresearchinra.academia.edu/VikasSharma">Vikas Sharma</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Viruses</p><p class="ds-related-work--abstract ds2-5-body-sm">The genus of Xanthomonas contains many well-known plant pathogens with the ability to infect some of the most important crop plants, thereby causing significant economic damage. Unfortunately, classical pest-control strategies are neither particularly efficient nor sustainable and we are, therefore, in demand of alternatives. Here, we present the isolation and characterization of seven novel phages infecting the plant-pathogenic species Xanthomonas translucens and Xanthomonas campestris. Transmission electron microscopy revealed that all phages show a siphovirion morphology. The analysis of genome sequences and plaque morphologies are in agreement with a lytic lifestyle of the phages making them suitable candidates for biocontrol. Moreover, three of the isolated phages form the new genus “Shirevirus”. All seven phages belong to four distinct clusters underpinning their phylogenetic diversity. Altogether, this study presents the first characterized isolates for the plant pathogen X. ...</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Isolation of Novel Xanthomonas Phages Infecting the Plant Pathogens X. translucens and X. campestris","attachmentId":89222692,"attachmentType":"pdf","work_url":"https://www.academia.edu/84078887/Isolation_of_Novel_Xanthomonas_Phages_Infecting_the_Plant_Pathogens_X_translucens_and_X_campestris","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/84078887/Isolation_of_Novel_Xanthomonas_Phages_Infecting_the_Plant_Pathogens_X_translucens_and_X_campestris"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="6" data-entity-id="23864849" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/23864849/Genome_of_Xanthomonas_oryzae_Bacteriophage_Xp10_An_Odd_T_Odd_Phage">Genome of Xanthomonas oryzae Bacteriophage Xp10: An Odd T-Odd Phage</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="46128569" href="https://independent.academia.edu/KonstantinSeverinov">Konstantin Severinov</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Journal of Molecular Biology, 2003</p><p class="ds-related-work--abstract ds2-5-body-sm">Xp10 is a lytic bacteriophage of the phytopathogenic bacterium Xanthomonas oryzae. Though morphologically Xp10 belongs to the Syphoviridae family, it encodes its own single-subunit RNA polymerase characteristic of T7-like phages of the Podoviridae family. Here, we report the determination and analysis of the 44,373 bp sequence of the Xp10 genome. The genome is a linear, double-stranded DNA molecule with 3 0 cohesive overhangs and no terminal repeats or redundancies. Half of the Xp10 genome contains genes coding for structural proteins and host lysis functions in an arrangement typical for temperate dairy phages that are related to the Escherichia coli l phage. The other half of the Xp10 genome contains genes coding for factors of host gene expression shut-off, enzymes of viral genome replication and expression. The two groups of genes are transcribed divergently and separated by a regulatory region, which contains divergent promoters recognized by the host RNA polymerase. Xp10 has apparently arisen through a recombination between genomes of widely different phages. Further evidence of extensive gene flux in the evolution of Xp10 includes a high fraction (10%) of genes derived from an HNH-family endonuclease, and a DNA-dependent DNA polymerase that is closer to a homolog from Leishmania than to DNA polymerases from other phages or bacteria.</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Genome of Xanthomonas oryzae Bacteriophage Xp10: An Odd T-Odd Phage","attachmentId":44257857,"attachmentType":"pdf","work_url":"https://www.academia.edu/23864849/Genome_of_Xanthomonas_oryzae_Bacteriophage_Xp10_An_Odd_T_Odd_Phage","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/23864849/Genome_of_Xanthomonas_oryzae_Bacteriophage_Xp10_An_Odd_T_Odd_Phage"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="7" data-entity-id="125027998" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/125027998/Isolation_and_genomic_characterization_of_the_first_phage_infecting_Iodobacteria_%CF%86PLPE_a_myovirus_having_a_novel_set_of_features">Isolation and genomic characterization of the first phage infecting Iodobacteria: ϕPLPE, a myovirus having a novel set of features</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="42157029" href="https://independent.academia.edu/EStockfleth">E. Stockfleth</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Environmental Microbiology Reports, 2009</p><p class="ds-related-work--abstract ds2-5-body-sm">SummaryThe aquatic phage ϕPLPE infects a bacterium of the genus Iodobacter that are common inhabitants of rivers, streams and canals that produce violacein‐like pigments. Our characterization of ϕPLPE reveals it to be a small, contractile‐tailed phage whose 47.5 kb genome sequence is phylogenetically distant from all previously characterized phages. The genome has a generally modular organization (e.g. replication/recombination, structure/morphogenesis, lysis/lysogeny) and approximately half of its 84 open reading frames have no known homologues. It behaves as a virulent phage under the host growth conditions we have employed and, with the exception of an anti‐repressor (ant) homologue, the genome lacks all the genes associated with a lysogenic lifestyle. Thus, either ϕPLPE was once a temperate phage that has lost most of its lysogeny cassette or it is a virulent phage that acquired an ant‐like gene presumably for some function other than the control of lysogeny. The ϕPLPE genome ha...</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Isolation and genomic characterization of the first phage infecting Iodobacteria: ϕPLPE, a myovirus having a novel set of features","attachmentId":119145395,"attachmentType":"pdf","work_url":"https://www.academia.edu/125027998/Isolation_and_genomic_characterization_of_the_first_phage_infecting_Iodobacteria_%CF%86PLPE_a_myovirus_having_a_novel_set_of_features","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/125027998/Isolation_and_genomic_characterization_of_the_first_phage_infecting_Iodobacteria_%CF%86PLPE_a_myovirus_having_a_novel_set_of_features"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="8" data-entity-id="64323752" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/64323752/Complete_Genome_Sequence_of_XaF13_a_Novel_Bacteriophage_of_named_content_content_type_genus_species_Xanthomonas_vesicatoria_named_content_from_Mexico">Complete Genome Sequence of XaF13, a Novel Bacteriophage of <named-content content-type='genus-species'>Xanthomonas vesicatoria</named-content> from Mexico</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="6236391" href="https://independent.academia.edu/GabrielRINCONENRIQUEZ">Gabriel RINCON-ENRIQUEZ</a></div><p class="ds-related-work--metadata ds2-5-body-xs">2020</p><p class="ds-related-work--abstract ds2-5-body-sm">The phytopathogenic bacterium Xanthomonas vesicatoria is the causative agent of bacterial spot disease in various Solanaceae family members. Here, we describe the complete genome sequence of XaF13, a novel filamentous phage that infects the phytopathogenic bacterium X. vesicatoria. The 7,045-bp genome is predicted to encode 14 open reading frames, 7 of which are related to those of other filamentous Xanthomonas phages. Mexico is the second most important producer of peppers worldwide. The production of peppers faces many phytosanitary problems such as bacterial spot disease (1–3). Bacterial spot disease control is difficult because Xanthomonas species can survive for long periods in seeds and crop debris (4). The use of bacteriophages against bacteria is considered a possible alternative to agrochemicals (5). The bacteriophage XaF13 (family Inoviridae) was isolated from a soil sample collected in Yurécuaro, Mexico, from a pepper field affected with bacterial spot disease. A soil sam...</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Complete Genome Sequence of XaF13, a Novel Bacteriophage of \u003cnamed-content content-type='genus-species'\u003eXanthomonas vesicatoria\u003c/named-content\u003e from Mexico","attachmentId":76410029,"attachmentType":"pdf","work_url":"https://www.academia.edu/64323752/Complete_Genome_Sequence_of_XaF13_a_Novel_Bacteriophage_of_named_content_content_type_genus_species_Xanthomonas_vesicatoria_named_content_from_Mexico","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/64323752/Complete_Genome_Sequence_of_XaF13_a_Novel_Bacteriophage_of_named_content_content_type_genus_species_Xanthomonas_vesicatoria_named_content_from_Mexico"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="9" data-entity-id="29941073" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/29941073/Isolation_and_Characterization_of_Xanthomonas_Euvesicatoriabacteriophages">Isolation and Characterization of Xanthomonas Euvesicatoriabacteriophages</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="56864603" href="https://independent.academia.edu/MajaIgnjatov">Maja V Ignjatov</a></div><p class="ds-related-work--metadata ds2-5-body-xs">JOURNAL OF PLANT PATHOLOGY</p><p class="ds-related-work--abstract ds2-5-body-sm">Host range, plaque morphology, thermal inactivation point, genome size and restriction fragment patterns of ten bacteriophage isolates originating from soil, pepper seed and irrigation water collected from five localities in Serbia were studied. The bacteriophage isolates were se-lected based on their specificity to Xanthomonas euvesi-catoria, causal agent of bacterial spot of pepper. The phages had similar plaque morphology, except for two isolates producing a plaque-surrounding halo in culture of X. euvesicatoria strain KFB 189. Four phage isolates were inactivated at 70ºC and six at 71ºC. All phages had genome size of approximately 22 kb and were differen-tiated into four types by their EcoRI and BamHI restric-tion fragment patterns. Examination of two phages by transmission electron microscopy classified them as A1 morphotype members of the Myoviridae family, order Caudovirales. Although specific to X. euvesicatoria, the phages were differentiated into three groups based on thei...</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Isolation and Characterization of Xanthomonas Euvesicatoriabacteriophages","attachmentId":50399157,"attachmentType":"pdf","work_url":"https://www.academia.edu/29941073/Isolation_and_Characterization_of_Xanthomonas_Euvesicatoriabacteriophages","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/29941073/Isolation_and_Characterization_of_Xanthomonas_Euvesicatoriabacteriophages"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div></div></div><div class="ds-sticky-ctas--wrapper js-loswp-sticky-ctas hidden"><div class="ds-sticky-ctas--grid-container"><div class="ds-sticky-ctas--container"><button class="ds2-5-button js-swp-download-button" data-signup-modal="{"location":"continue-reading-button--sticky-ctas","attachmentId":91268320,"attachmentType":"pdf","workUrl":null}">See full PDF</button><button class="ds2-5-button ds2-5-button--secondary js-swp-download-button" data-signup-modal="{"location":"download-pdf-button--sticky-ctas","attachmentId":91268320,"attachmentType":"pdf","workUrl":null}"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">download</span>Download PDF</button></div></div></div><div class="ds-below-fold--grid-container"><div class="ds-work--container js-loswp-embedded-document"><div class="attachment_preview" data-attachment="Attachment_91268320" style="display: none"><div class="js-scribd-document-container"><div class="scribd--document-loading js-scribd-document-loader" style="display: block;"><img alt="Loading..." src="//a.academia-assets.com/images/loaders/paper-load.gif" /><p>Loading Preview</p></div></div><div style="text-align: center;"><div class="scribd--no-preview-alert js-preview-unavailable"><p>Sorry, preview is currently unavailable. You can download the paper by clicking the button above.</p></div></div></div></div><div class="ds-sidebar--container js-work-sidebar"><div class="ds-related-content--container"><h2 class="ds-related-content--heading">Related papers</h2><div class="ds-related-work--container js-related-work-sidebar-card" data-collection-position="0" data-entity-id="21975402" data-sort-order="default"><a class="ds-related-work--title js-related-work-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/21975402/Morphology_and_genome_sequence_of_phage_%CF%861402_A_dwarf_myovirus_of_the_predatory_bacterium_Bdellovibrio_bacteriovorus">Morphology and genome sequence of phage ϕ1402: A dwarf myovirus of the predatory bacterium Bdellovibrio bacteriovorus</a><div class="ds-related-work--metadata"><a class="js-related-work-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="43234150" href="https://independent.academia.edu/HenryKrisch">Henry Krisch</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Bacteriophage, 2011</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Morphology and genome sequence of phage ϕ1402: A dwarf myovirus of the predatory bacterium Bdellovibrio bacteriovorus","attachmentId":42688843,"attachmentType":"pdf","work_url":"https://www.academia.edu/21975402/Morphology_and_genome_sequence_of_phage_%CF%861402_A_dwarf_myovirus_of_the_predatory_bacterium_Bdellovibrio_bacteriovorus","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-related-work-grid-card-view-pdf" href="https://www.academia.edu/21975402/Morphology_and_genome_sequence_of_phage_%CF%861402_A_dwarf_myovirus_of_the_predatory_bacterium_Bdellovibrio_bacteriovorus"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-related-work-sidebar-card" data-collection-position="1" data-entity-id="48021594" data-sort-order="default"><a class="ds-related-work--title js-related-work-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/48021594/Isolation_characterization_and_complete_genome_sequence_of_PhaxI_a_phage_of_Escherichia_coli_O157_H7">Isolation, characterization and complete genome sequence of PhaxI: a phage of Escherichia coli O157 : H7</a><div class="ds-related-work--metadata"><a class="js-related-work-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="32272130" 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