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Rezaie</title> <meta name="description" content="Search results for: Behrooz Rezaie"> <meta name="keywords" content="Behrooz Rezaie"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/css/site.css?v=150220211555" rel="stylesheet"> </head> <body> <header> <div class="container"> <nav class="navbar navbar-expand-lg navbar-light"> <a class="navbar-brand" href="https://waset.org"> <img src="https://cdn.waset.org/static/images/wasetc.png" alt="Open Science Research Excellence" title="Open Science Research Excellence" /> </a> <button class="d-block d-lg-none navbar-toggler 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class="input-group"> <i class="fas fa-search"></i> <input type="text" class="search-query" name="q" placeholder="Author, Title, Abstract, Keywords" value="Behrooz Rezaie"> <input type="submit" class="btn_search" value="Search"> </div> </div> </form> </div> </div> <div class="row mt-3"> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Commenced</strong> in January 2007</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Frequency:</strong> Monthly</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Edition:</strong> International</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Paper Count:</strong> 13</div> </div> </div> </div> <h1 class="mt-3 mb-3 text-center" style="font-size:1.6rem;">Search results for: Behrooz Rezaie</h1> <div class="card publication-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">13</span> Stabilization of the Lorenz Chaotic Equations by Fuzzy Controller</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/search?q=Behrooz%20Rezaie">Behrooz Rezaie</a>, <a href="https://publications.waset.org/search?q=Zahra%20Rahmani%20Cherati"> Zahra Rahmani Cherati</a>, <a href="https://publications.waset.org/search?q=Mohammad%20Reza%20Jahed%20Motlagh"> Mohammad Reza Jahed Motlagh</a>, <a href="https://publications.waset.org/search?q=Mohammad%20Farrokhi"> Mohammad Farrokhi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In this paper, a fuzzy controller is designed for stabilization of the Lorenz chaotic equations. A simple Mamdani inference method is used for this purpose. This method is very simple and applicable for complex chaotic systems and it can be implemented easily. The stability of close loop system is investigated by the Lyapunov stabilization criterion. A Lyapunov function is introduced and the global stability is proven. Finally, the effectiveness of this method is illustrated by simulation results and it is shown that the performance of the system is improved. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/search?q=Chaotic%20system" title="Chaotic system">Chaotic system</a>, <a href="https://publications.waset.org/search?q=Fuzzy%20control" title=" Fuzzy control"> Fuzzy control</a>, <a href="https://publications.waset.org/search?q=Lorenz%20equation." title=" Lorenz equation."> Lorenz equation.</a> </p> <a href="https://publications.waset.org/1521/stabilization-of-the-lorenz-chaotic-equations-by-fuzzy-controller" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/1521/apa" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">APA</a> <a href="https://publications.waset.org/1521/bibtex" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">BibTeX</a> <a href="https://publications.waset.org/1521/chicago" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">Chicago</a> <a href="https://publications.waset.org/1521/endnote" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">EndNote</a> <a href="https://publications.waset.org/1521/harvard" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">Harvard</a> <a href="https://publications.waset.org/1521/json" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">JSON</a> <a href="https://publications.waset.org/1521/mla" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">MLA</a> <a href="https://publications.waset.org/1521/ris" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">RIS</a> <a href="https://publications.waset.org/1521/xml" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">XML</a> <a href="https://publications.waset.org/1521/iso690" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">ISO 690</a> <a href="https://publications.waset.org/1521.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">2029</span> </span> </div> </div> <div class="card publication-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">12</span> Solution of Two Dimensional Quasi-Harmonic Equations with CA Approach</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/search?q=F.%20Rezaie%20Moghaddam">F. Rezaie Moghaddam</a>, <a href="https://publications.waset.org/search?q=J.%20Amani"> J. Amani</a>, <a href="https://publications.waset.org/search?q=T.%20Rezaie%20Moghaddam"> T. Rezaie Moghaddam</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Many computational techniques were applied to solution of heat conduction problem. Those techniques were the finite difference (FD), finite element (FE) and recently meshless methods. FE is commonly used in solution of equation of heat conduction problem based on the summation of stiffness matrix of elements and the solution of the final system of equations. Because of summation process of finite element, convergence rate was decreased. Hence in the present paper Cellular Automata (CA) approach is presented for the solution of heat conduction problem. Each cell considered as a fixed point in a regular grid lead to the solution of a system of equations is substituted by discrete systems of equations with small dimensions. Results show that CA can be used for solution of heat conduction problem. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/search?q=Heat%20conduction" title="Heat conduction">Heat conduction</a>, <a href="https://publications.waset.org/search?q=Cellular%20automata" title=" Cellular automata"> Cellular automata</a>, <a href="https://publications.waset.org/search?q=convergencerate" title=" convergencerate"> convergencerate</a>, <a href="https://publications.waset.org/search?q=discrete%20system." title=" discrete system."> discrete system.</a> </p> <a href="https://publications.waset.org/471/solution-of-two-dimensional-quasi-harmonic-equations-with-ca-approach" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/471/apa" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">APA</a> <a href="https://publications.waset.org/471/bibtex" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">BibTeX</a> <a href="https://publications.waset.org/471/chicago" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">Chicago</a> <a href="https://publications.waset.org/471/endnote" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">EndNote</a> <a href="https://publications.waset.org/471/harvard" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">Harvard</a> <a href="https://publications.waset.org/471/json" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">JSON</a> <a href="https://publications.waset.org/471/mla" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">MLA</a> <a href="https://publications.waset.org/471/ris" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">RIS</a> <a href="https://publications.waset.org/471/xml" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">XML</a> <a href="https://publications.waset.org/471/iso690" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">ISO 690</a> <a href="https://publications.waset.org/471.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">1775</span> </span> </div> </div> <div class="card publication-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">11</span> Crash Severity Modeling in Urban Highways Using Backward Regression Method</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/search?q=F.%20Rezaie%20Moghaddam">F. Rezaie Moghaddam</a>, <a href="https://publications.waset.org/search?q=T.%20Rezaie%20Moghaddam"> T. Rezaie Moghaddam</a>, <a href="https://publications.waset.org/search?q=M.%20Pasbani%20Khiavi"> M. Pasbani Khiavi</a>, <a href="https://publications.waset.org/search?q=M.%20Ali%20Ghorbani"> M. Ali Ghorbani</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Identifying and classifying intersections according to severity is very important for implementation of safety related counter measures and effective models are needed to compare and assess the severity. Highway safety organizations have considered intersection safety among their priorities. In spite of significant advances in highways safety, the large numbers of crashes with high severities still occur in the highways. Investigation of influential factors on crashes enables engineers to carry out calculations in order to reduce crash severity. Previous studies lacked a model capable of simultaneous illustration of the influence of human factors, road, vehicle, weather conditions and traffic features including traffic volume and flow speed on the crash severity. Thus, this paper is aimed at developing the models to illustrate the simultaneous influence of these variables on the crash severity in urban highways. The models represented in this study have been developed using binary Logit Models. SPSS software has been used to calibrate the models. It must be mentioned that backward regression method in SPSS was used to identify the significant variables in the model. Consider to obtained results it can be concluded that the main factor in increasing of crash severity in urban highways are driver age, movement with reverse gear, technical defect of the vehicle, vehicle collision with motorcycle and bicycle, bridge, frontal impact collisions, frontal-lateral collisions and multi-vehicle crashes in urban highways which always increase the crash severity in urban highways. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/search?q=Backward%20regression" title="Backward regression">Backward regression</a>, <a href="https://publications.waset.org/search?q=crash%20severity" title=" crash severity"> crash severity</a>, <a href="https://publications.waset.org/search?q=speed" title=" speed"> speed</a>, <a href="https://publications.waset.org/search?q=urbanhighways." title=" urbanhighways."> urbanhighways.</a> </p> <a href="https://publications.waset.org/3237/crash-severity-modeling-in-urban-highways-using-backward-regression-method" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/3237/apa" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">APA</a> <a href="https://publications.waset.org/3237/bibtex" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">BibTeX</a> <a href="https://publications.waset.org/3237/chicago" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">Chicago</a> <a href="https://publications.waset.org/3237/endnote" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">EndNote</a> <a href="https://publications.waset.org/3237/harvard" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">Harvard</a> <a href="https://publications.waset.org/3237/json" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">JSON</a> <a href="https://publications.waset.org/3237/mla" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">MLA</a> <a href="https://publications.waset.org/3237/ris" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">RIS</a> <a href="https://publications.waset.org/3237/xml" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">XML</a> <a href="https://publications.waset.org/3237/iso690" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">ISO 690</a> <a href="https://publications.waset.org/3237.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">1921</span> </span> </div> </div> <div class="card publication-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">10</span> Ranking DMUs by Ideal PPS in Data Envelopment Analysis</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/search?q=V.Rezaie">V.Rezaie</a>, <a href="https://publications.waset.org/search?q=M.Khanmohammady"> M.Khanmohammady</a> </p> <p class="card-text"><strong>Abstract:</strong></p> An original DEA model is to evaluate each DMU optimistically, but the interval DEA Model proposed in this paper has been formulated to obtain an efficiency interval consisting of Evaluations from both the optimistic and the pessimistic view points. DMUs are improved so that their lower bounds become so large as to attain the maximum Value one. The points obtained by this method are called ideal points. Ideal PPS is calculated by ideal of efficiency DMUs. The purpose of this paper is to rank DMUs by this ideal PPS. Finally we extend the efficiency interval of a DMU under variable RTS technology. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/search?q=Data%20envelopment%20analysis%20%28DEA%29" title="Data envelopment analysis (DEA)">Data envelopment analysis (DEA)</a>, <a href="https://publications.waset.org/search?q=Decision%20makingunit%20%28DMU%29" title=" Decision makingunit (DMU)"> Decision makingunit (DMU)</a>, <a href="https://publications.waset.org/search?q=Interval%20DEA" title=" Interval DEA"> Interval DEA</a>, <a href="https://publications.waset.org/search?q=Ideal%20points" title=" Ideal points"> Ideal points</a>, <a href="https://publications.waset.org/search?q=Ideal%20PPS" title=" Ideal PPS"> Ideal PPS</a>, <a href="https://publications.waset.org/search?q=Return%20to%20scale%28RTS%29." title=" Return to scale(RTS)."> Return to scale(RTS).</a> </p> <a href="https://publications.waset.org/12/ranking-dmus-by-ideal-pps-in-data-envelopment-analysis" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/12/apa" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">APA</a> <a href="https://publications.waset.org/12/bibtex" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">BibTeX</a> <a href="https://publications.waset.org/12/chicago" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">Chicago</a> <a href="https://publications.waset.org/12/endnote" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">EndNote</a> <a href="https://publications.waset.org/12/harvard" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">Harvard</a> <a href="https://publications.waset.org/12/json" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">JSON</a> <a href="https://publications.waset.org/12/mla" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">MLA</a> <a href="https://publications.waset.org/12/ris" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">RIS</a> <a href="https://publications.waset.org/12/xml" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">XML</a> <a href="https://publications.waset.org/12/iso690" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">ISO 690</a> <a href="https://publications.waset.org/12.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">1926</span> </span> </div> </div> <div class="card publication-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">9</span> Microwave LNA Design Based On Adaptive Network Fuzzy Inference and Evolutionary Optimization</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/search?q=Samad%20Nejatian">Samad Nejatian</a>, <a href="https://publications.waset.org/search?q=Vahideh%20Rezaie"> Vahideh Rezaie</a>, <a href="https://publications.waset.org/search?q=Vahid%20Asadpour"> Vahid Asadpour</a> </p> <p class="card-text"><strong>Abstract:</strong></p> This paper presents a novel approach for the design of microwave circuits using Adaptive Network Fuzzy Inference Optimizer (ANFIO). The method takes advantage of direct synthesis of subsections of the amplifier using very fast and accurate ANFIO models based on exact simulations using ADS. A mapping from course space to fine space known as space mapping is also used. The proposed synthesis approach takes into account the noise and scattering parameters due to parasitic elements to achieve optimal results. The overall ANFIO system is capable of designing different LNAs at different noise and scattering criteria. This approach offers significantly reduced time in the design of microwave amplifiers within the validity range of the ANFIO system. The method has been proven to work efficiently for a 2.4GHz LNA example. The S21 of 10.1 dB and noise figure (NF) of 2.7 dB achieved for ANFIO while S21 of 9.05 dB and NF of 2.6 dB achieved for ANN. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/search?q=fuzzy%20system" title="fuzzy system">fuzzy system</a>, <a href="https://publications.waset.org/search?q=low%20noise%20amplifier" title=" low noise amplifier"> low noise amplifier</a>, <a href="https://publications.waset.org/search?q=microwaveamplifier" title=" microwaveamplifier"> microwaveamplifier</a>, <a href="https://publications.waset.org/search?q=space%20mapping" title=" space mapping"> space mapping</a> </p> <a href="https://publications.waset.org/14634/microwave-lna-design-based-on-adaptive-network-fuzzy-inference-and-evolutionary-optimization" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/14634/apa" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">APA</a> <a href="https://publications.waset.org/14634/bibtex" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">BibTeX</a> <a href="https://publications.waset.org/14634/chicago" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">Chicago</a> <a href="https://publications.waset.org/14634/endnote" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">EndNote</a> <a href="https://publications.waset.org/14634/harvard" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">Harvard</a> <a href="https://publications.waset.org/14634/json" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">JSON</a> <a href="https://publications.waset.org/14634/mla" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">MLA</a> <a href="https://publications.waset.org/14634/ris" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">RIS</a> <a href="https://publications.waset.org/14634/xml" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">XML</a> <a href="https://publications.waset.org/14634/iso690" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">ISO 690</a> <a href="https://publications.waset.org/14634.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">1796</span> </span> </div> </div> <div class="card publication-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">8</span> Evaluation of Dynamic Behavior of a Rotor-Bearing System in Operating Conditions</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/search?q=Mohammad%20Hadi%20Jalali">Mohammad Hadi Jalali</a>, <a href="https://publications.waset.org/search?q=Behrooz%20Shahriari"> Behrooz Shahriari</a>, <a href="https://publications.waset.org/search?q=Mostafa%20Ghayour"> Mostafa Ghayour</a>, <a href="https://publications.waset.org/search?q=Saeed%20Ziaei-Rad"> Saeed Ziaei-Rad</a>, <a href="https://publications.waset.org/search?q=Shahram%20Yousefi"> Shahram Yousefi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> <p>Most flexible rotors can be considered as beam-like structures. In many cases, rotors are modeled as one-dimensional bodies, made basically of beam-like shafts with rigid bodies attached to them. This approach is typical of rotor dynamics, both analytical and numerical, and several rotor dynamic codes, based on the finite element method, follow this trend. In this paper, a finite element model based on Timoshenko beam elements is utilized to analyze the lateral dynamic behavior of a certain rotor-bearing system in operating conditions.</p> <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/search?q=Finite%20element%20method" title="Finite element method">Finite element method</a>, <a href="https://publications.waset.org/search?q=Operational%20deflection%20shape" title=" Operational deflection shape"> Operational deflection shape</a>, <a href="https://publications.waset.org/search?q=Timoshenko%20beam%20elements" title=" Timoshenko beam elements"> Timoshenko beam elements</a>, <a href="https://publications.waset.org/search?q=Unbalance%20response." title=" Unbalance response."> Unbalance response.</a> </p> <a href="https://publications.waset.org/9999477/evaluation-of-dynamic-behavior-of-a-rotor-bearing-system-in-operating-conditions" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/9999477/apa" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">APA</a> <a href="https://publications.waset.org/9999477/bibtex" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">BibTeX</a> <a href="https://publications.waset.org/9999477/chicago" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">Chicago</a> <a href="https://publications.waset.org/9999477/endnote" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">EndNote</a> <a href="https://publications.waset.org/9999477/harvard" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">Harvard</a> <a href="https://publications.waset.org/9999477/json" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">JSON</a> <a href="https://publications.waset.org/9999477/mla" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">MLA</a> <a href="https://publications.waset.org/9999477/ris" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">RIS</a> <a href="https://publications.waset.org/9999477/xml" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">XML</a> <a href="https://publications.waset.org/9999477/iso690" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">ISO 690</a> <a href="https://publications.waset.org/9999477.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">3010</span> </span> </div> </div> <div class="card publication-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">7</span> Electrode Engineering for On-Chip Liquid Driving by Using Electrokinetic Effect</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/search?q=Reza%20Hadjiaghaie%20Vafaie">Reza Hadjiaghaie Vafaie</a>, <a href="https://publications.waset.org/search?q=Aysan%20Madanpasandi"> Aysan Madanpasandi</a>, <a href="https://publications.waset.org/search?q=Behrooz%20Zare%20Desari"> Behrooz Zare Desari</a>, <a href="https://publications.waset.org/search?q=Seyedmohammad%20Mousavi"> Seyedmohammad Mousavi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> <p>High lamination in microchannel is one of the main challenges in on-chip components like micro total analyzer systems and lab-on-a-chips. Electro-osmotic force is highly effective in chip-scale. This research proposes a microfluidic-based micropump for low ionic strength solutions. Narrow microchannels are designed to generate an efficient electroosmotic flow near the walls. Microelectrodes are embedded in the lateral sides and actuated by low electric potential to generate pumping effect inside the channel. Based on the simulation study, the fluid velocity increases by increasing the electric potential amplitude. We achieve a net flow velocity of 100 µm/s, by applying +/- 2 V to the electrode structures. Our proposed low voltage design is of interest in conventional lab-on-a-chip applications.</p> <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/search?q=Integration" title="Integration">Integration</a>, <a href="https://publications.waset.org/search?q=electrokinetic" title=" electrokinetic"> electrokinetic</a>, <a href="https://publications.waset.org/search?q=on-chip" title=" on-chip"> on-chip</a>, <a href="https://publications.waset.org/search?q=fluid%20pumping" title=" fluid pumping"> fluid pumping</a>, <a href="https://publications.waset.org/search?q=microfluidic." title=" microfluidic. "> microfluidic. </a> </p> <a href="https://publications.waset.org/10007738/electrode-engineering-for-on-chip-liquid-driving-by-using-electrokinetic-effect" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/10007738/apa" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">APA</a> <a href="https://publications.waset.org/10007738/bibtex" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">BibTeX</a> <a href="https://publications.waset.org/10007738/chicago" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">Chicago</a> <a href="https://publications.waset.org/10007738/endnote" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">EndNote</a> <a href="https://publications.waset.org/10007738/harvard" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">Harvard</a> <a href="https://publications.waset.org/10007738/json" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">JSON</a> <a href="https://publications.waset.org/10007738/mla" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">MLA</a> <a href="https://publications.waset.org/10007738/ris" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">RIS</a> <a href="https://publications.waset.org/10007738/xml" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">XML</a> <a href="https://publications.waset.org/10007738/iso690" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">ISO 690</a> <a href="https://publications.waset.org/10007738.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">844</span> </span> </div> </div> <div class="card publication-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">6</span> Nonlinear Analysis of Shear Wall Using Finite Element Model</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/search?q=M.%20A.%20Ghorbani">M. A. Ghorbani</a>, <a href="https://publications.waset.org/search?q=M.%20Pasbani%20Khiavi"> M. Pasbani Khiavi</a>, <a href="https://publications.waset.org/search?q=F.%20Rezaie%20Moghaddam"> F. Rezaie Moghaddam</a> </p> <p class="card-text"><strong>Abstract:</strong></p> <p>In the analysis of structures, the nonlinear effects due to large displacement, large rotation and materially-nonlinear are very important and must be considered for the reliable analysis. The non-linear fmite element analysis has potential as usable and reliable means for analyzing of civil structures with the availability of computer technology. In this research the large displacements and materially nonlinear behavior of shear wall is presented with developing of fmite element code using the standard Galerkin weighted residual formulation. Two-dimensional plane stress model was carried out to present the shear wall response. Total Lagangian formulation, which is computationally more effective, is used in the formulation of stiffness matrices and the Newton-Raphson method is applied for the solution of nonlinear transient equations. The details of the program formulation are highlighted and the results of the analyses are presented, along with a comparison of the response of the structure with Ansys software results. The presented model in this paper can be developed for nonlinear analysis of civil engineering structures with different material behavior and complicated geometry.</p> <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/search?q=Finite%20element" title="Finite element">Finite element</a>, <a href="https://publications.waset.org/search?q=large%20displacements" title=" large displacements"> large displacements</a>, <a href="https://publications.waset.org/search?q=materially%20nonlinear" title=" materially nonlinear"> materially nonlinear</a>, <a href="https://publications.waset.org/search?q=shear%20wall." title=" shear wall."> shear wall.</a> </p> <a href="https://publications.waset.org/4254/nonlinear-analysis-of-shear-wall-using-finite-element-model" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/4254/apa" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">APA</a> <a href="https://publications.waset.org/4254/bibtex" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">BibTeX</a> <a href="https://publications.waset.org/4254/chicago" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">Chicago</a> <a href="https://publications.waset.org/4254/endnote" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">EndNote</a> <a href="https://publications.waset.org/4254/harvard" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">Harvard</a> <a href="https://publications.waset.org/4254/json" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">JSON</a> <a href="https://publications.waset.org/4254/mla" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">MLA</a> <a href="https://publications.waset.org/4254/ris" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">RIS</a> <a href="https://publications.waset.org/4254/xml" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">XML</a> <a href="https://publications.waset.org/4254/iso690" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">ISO 690</a> <a href="https://publications.waset.org/4254.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">1754</span> </span> </div> </div> <div class="card publication-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">5</span> Chemical Composition of Essential Oil and in vitro Antibacterial and Anticancer Activity of the Hydroalcolic Extract from Coronilla varia</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/search?q=Dehpour%20A.%20A.">Dehpour A. A.</a>, <a href="https://publications.waset.org/search?q=Eslami%20B."> Eslami B.</a>, <a href="https://publications.waset.org/search?q=Rezaie%20S."> Rezaie S.</a>, <a href="https://publications.waset.org/search?q=Hashemian%20S.%20F."> Hashemian S. F.</a>, <a href="https://publications.waset.org/search?q=Shafie%20F."> Shafie F.</a>, <a href="https://publications.waset.org/search?q=Kiaie%20M."> Kiaie M.</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The aims of study were investigation on chemical composition essential oil and the effect of extract of Coronilla varia on antimicrobial and cytotoxicity activity. The essential oils of Coronilla varia is obtained by hydrodistillation and analyzed by (GC/MS) for determining their chemical composition and identification of their components. Antibacterial activity of plant extract was determined by disc diffusion method and anticancer activity measured by MTT assay. The major components in essential oil were Caryophyllene Oxide (60.19%), Alphacadinol (4.13%) and Homoadantaneca Robexylic Acid (3.31%). The extracts from Coronilla varia had interesting activity against Proteus mirabilis in the concentration of 700 μg/disc and did not show any activity against Staphylococus aureus, Bacillus subtillis, Klebsiella pneumonia and Entrobacter cloacae. The positive control, Ampicillin, Chloramphenicol and Cenphalothin had shown zone of inhibition resistant all bacteria. The ethanol extract of Corohilla varia inhibited on MCF7 cell lines. IC50 0.6(mg/ml) was the optimum concentration of extract from Coronilla varia inhibition of cell line growth. The MCF7 cancer cell line and Proteus mirabilis were more sensitive to Coronilla varia ethanol extract. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/search?q=Coronilla%20varia" title="Coronilla varia">Coronilla varia</a>, <a href="https://publications.waset.org/search?q=Essential%20oil" title=" Essential oil"> Essential oil</a>, <a href="https://publications.waset.org/search?q=Antibacterial" title=" Antibacterial"> Antibacterial</a>, <a href="https://publications.waset.org/search?q=Anticancer" title=" Anticancer"> Anticancer</a>, <a href="https://publications.waset.org/search?q=HeLa%20cell%20line." title=" HeLa cell line."> HeLa cell line.</a> </p> <a href="https://publications.waset.org/10002400/chemical-composition-of-essential-oil-and-in-vitro-antibacterial-and-anticancer-activity-of-the-hydroalcolic-extract-from-coronilla-varia" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/10002400/apa" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">APA</a> <a href="https://publications.waset.org/10002400/bibtex" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">BibTeX</a> <a href="https://publications.waset.org/10002400/chicago" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">Chicago</a> <a href="https://publications.waset.org/10002400/endnote" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">EndNote</a> <a href="https://publications.waset.org/10002400/harvard" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">Harvard</a> <a href="https://publications.waset.org/10002400/json" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">JSON</a> <a href="https://publications.waset.org/10002400/mla" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">MLA</a> <a href="https://publications.waset.org/10002400/ris" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">RIS</a> <a href="https://publications.waset.org/10002400/xml" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">XML</a> <a href="https://publications.waset.org/10002400/iso690" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">ISO 690</a> <a href="https://publications.waset.org/10002400.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">1797</span> </span> </div> </div> <div class="card publication-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4</span> Combined Safety and Cybersecurity Risk Assessment for Intelligent Distributed Grids</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/search?q=Anders%20Thors%C3%A8n">Anders Thorsèn</a>, <a href="https://publications.waset.org/search?q=Behrooz%20Sangchoolie"> Behrooz Sangchoolie</a>, <a href="https://publications.waset.org/search?q=Peter%20Folkesson"> Peter Folkesson</a>, <a href="https://publications.waset.org/search?q=Ted%20Strandberg"> Ted Strandberg</a> </p> <p class="card-text"><strong>Abstract:</strong></p> <p>As more parts of the power grid become connected to the internet, the risk of cyberattacks increases. To identify the cybersecurity threats and subsequently reduce vulnerabilities, the common practice is to carry out a cybersecurity risk assessment. For safety classified systems and products, there is also a need for safety risk assessments in addition to the cybersecurity risk assessment to identify and reduce safety risks. These two risk assessments are usually done separately, but since cybersecurity and functional safety are often related, a more comprehensive method covering both aspects is needed. Some work addressing this has been done for specific domains like the automotive domain, but more general methods suitable for, e.g., Intelligent Distributed Grids, are still missing. One such method from the automotive domain is the Security-Aware Hazard Analysis and Risk Assessment (SAHARA) method that combines safety and cybersecurity risk assessments. This paper presents an approach where the SAHARA method has been modified to be more suitable for larger distributed systems. The adapted SAHARA method has a more general risk assessment approach than the original SAHARA. The proposed method has been successfully applied on two use cases of an intelligent distributed grid.</p> <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/search?q=Intelligent%20distribution%20grids" title="Intelligent distribution grids">Intelligent distribution grids</a>, <a href="https://publications.waset.org/search?q=threat%20analysis" title=" threat analysis"> threat analysis</a>, <a href="https://publications.waset.org/search?q=risk%0D%0Aassessment" title=" risk assessment"> risk assessment</a>, <a href="https://publications.waset.org/search?q=safety" title=" safety"> safety</a>, <a href="https://publications.waset.org/search?q=cybersecurity." title=" cybersecurity."> cybersecurity.</a> </p> <a href="https://publications.waset.org/10012552/combined-safety-and-cybersecurity-risk-assessment-for-intelligent-distributed-grids" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/10012552/apa" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">APA</a> <a href="https://publications.waset.org/10012552/bibtex" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">BibTeX</a> <a href="https://publications.waset.org/10012552/chicago" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">Chicago</a> <a href="https://publications.waset.org/10012552/endnote" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">EndNote</a> <a href="https://publications.waset.org/10012552/harvard" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">Harvard</a> <a href="https://publications.waset.org/10012552/json" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">JSON</a> <a href="https://publications.waset.org/10012552/mla" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">MLA</a> <a href="https://publications.waset.org/10012552/ris" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">RIS</a> <a href="https://publications.waset.org/10012552/xml" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">XML</a> <a href="https://publications.waset.org/10012552/iso690" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">ISO 690</a> <a href="https://publications.waset.org/10012552.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">757</span> </span> </div> </div> <div class="card publication-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3</span> Mechanical Investigation Approach to Optimize the High-Velocity Oxygen Fuel Fe-Based Amorphous Coatings Reinforced by B4C Nanoparticles</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/search?q=Behrooz%20Movahedi">Behrooz Movahedi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> <p>Fe-based amorphous feedstock powders are used as the matrix into which various ratios of hard B4C nanoparticles (0, 5, 10, 15, 20 vol.%) as reinforcing agents were prepared using a planetary high-energy mechanical milling. The ball-milled nanocomposite feedstock powders were also sprayed by means of high-velocity oxygen fuel (HVOF) technique. The characteristics of the powder particles and the prepared coating depending on their microstructures and nanohardness were examined in detail using nanoindentation tester. The results showed that the formation of the Fe-based amorphous phase was noticed over the course of high-energy ball milling. It is interesting to note that the nanocomposite coating is divided into two regions, namely, a full amorphous phase region and homogeneous dispersion of B4C nanoparticles with a scale of 10–50 nm in a residual amorphous matrix. As the B4C content increases, the nanohardness of the composite coatings increases, but the fracture toughness begins to decrease at the B4C content higher than 20 vol.%. The optimal mechanical properties are obtained with 15 vol.% B4C due to the suitable content and uniform distribution of nanoparticles. Consequently, the changes in mechanical properties of the coatings were attributed to the changes in the brittle to ductile transition by adding B4C nanoparticles.</p> <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/search?q=Fe-based%20amorphous" title="Fe-based amorphous">Fe-based amorphous</a>, <a href="https://publications.waset.org/search?q=B4C%20nanoparticles" title=" B4C nanoparticles"> B4C nanoparticles</a>, <a href="https://publications.waset.org/search?q=nanocomposite%20coating" title=" nanocomposite coating"> nanocomposite coating</a>, <a href="https://publications.waset.org/search?q=HVOF." title=" HVOF."> HVOF.</a> </p> <a href="https://publications.waset.org/10009493/mechanical-investigation-approach-to-optimize-the-high-velocity-oxygen-fuel-fe-based-amorphous-coatings-reinforced-by-b4c-nanoparticles" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/10009493/apa" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">APA</a> <a href="https://publications.waset.org/10009493/bibtex" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">BibTeX</a> <a href="https://publications.waset.org/10009493/chicago" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">Chicago</a> <a href="https://publications.waset.org/10009493/endnote" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">EndNote</a> <a href="https://publications.waset.org/10009493/harvard" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">Harvard</a> <a href="https://publications.waset.org/10009493/json" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">JSON</a> <a href="https://publications.waset.org/10009493/mla" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">MLA</a> <a href="https://publications.waset.org/10009493/ris" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">RIS</a> <a href="https://publications.waset.org/10009493/xml" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">XML</a> <a href="https://publications.waset.org/10009493/iso690" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">ISO 690</a> <a href="https://publications.waset.org/10009493.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">743</span> </span> </div> </div> <div class="card publication-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2</span> Optimum Locations for Intercity Bus Terminals with the AHP Approach – Case Study of the City of Esfahan</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/search?q=Mehrdad%20Arabi">Mehrdad Arabi</a>, <a href="https://publications.waset.org/search?q=Ehsan%20Beheshtitabar"> Ehsan Beheshtitabar</a>, <a href="https://publications.waset.org/search?q=Bahador%20Ghadirifaraz"> Bahador Ghadirifaraz</a>, <a href="https://publications.waset.org/search?q=Behrooz%20Forjanizadeh"> Behrooz Forjanizadeh</a> </p> <p class="card-text"><strong>Abstract:</strong></p> <p>Interaction between human, location and activity defines space. In the framework of these relations, space is a container for current specifications in relations of the 3 mentioned elements. The change of land utility considered with average performance range, urban regulations, society requirements etc. will provide welfare and comfort for citizens. From an engineering view it is fundamental that choosing a proper location for a specific civil activity requires evaluation of locations from different perspectives. The debate of desirable establishment of municipal service elements in urban regions is one of the most important issues related to urban planning. In this paper, the research type is applicable based on goal, and is descriptive and analytical based on nature. Initially existing terminals in Esfahan are surveyed and then new locations are presented based on evaluated criteria. In order to evaluate terminals based on the considered factors, an AHP model is used at first to estimate weight of different factors and then existing and suggested locations are evaluated using Arc GIS software and AHP model results. The results show that existing bus terminals are located in fairly proper locations. Further results of this study suggest new locations to establish terminals based on urban criteria.</p> <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/search?q=Arc%20GIS" title="Arc GIS">Arc GIS</a>, <a href="https://publications.waset.org/search?q=Esfahan%20city" title=" Esfahan city"> Esfahan city</a>, <a href="https://publications.waset.org/search?q=Optimum%20locations" title=" Optimum locations"> Optimum locations</a>, <a href="https://publications.waset.org/search?q=Terminals." title=" Terminals."> Terminals.</a> </p> <a href="https://publications.waset.org/10000590/optimum-locations-for-intercity-bus-terminals-with-the-ahp-approach-case-study-of-the-city-of-esfahan" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/10000590/apa" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">APA</a> <a href="https://publications.waset.org/10000590/bibtex" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">BibTeX</a> <a href="https://publications.waset.org/10000590/chicago" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">Chicago</a> <a href="https://publications.waset.org/10000590/endnote" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">EndNote</a> <a href="https://publications.waset.org/10000590/harvard" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">Harvard</a> <a href="https://publications.waset.org/10000590/json" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">JSON</a> <a href="https://publications.waset.org/10000590/mla" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">MLA</a> <a href="https://publications.waset.org/10000590/ris" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">RIS</a> <a href="https://publications.waset.org/10000590/xml" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">XML</a> <a href="https://publications.waset.org/10000590/iso690" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">ISO 690</a> <a href="https://publications.waset.org/10000590.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">2504</span> </span> </div> </div> <div class="card publication-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1</span> A Novel Multiplex Real-Time PCR Assay Using TaqMan MGB Probes for Rapid Detection of Trisomy 21</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/search?q=Mehrdad%20Hashemi">Mehrdad Hashemi</a>, <a href="https://publications.waset.org/search?q=Mitra%20Behrooz%20Aghdam"> Mitra Behrooz Aghdam</a>, <a href="https://publications.waset.org/search?q=Reza%20Mahdian"> Reza Mahdian</a>, <a href="https://publications.waset.org/search?q=Ahmad%20Reza%20Kamyab"> Ahmad Reza Kamyab</a> </p> <p class="card-text"><strong>Abstract:</strong></p> <p>Cytogenetic analysis still remains the gold standard method for prenatal diagnosis of trisomy 21 (Down syndrome, DS). Nevertheless, the conventional cytogenetic analysis needs live cultured cells and is too time-consuming for clinical application. In contrast, molecular methods such as FISH, QF-PCR, MLPA and quantitative Real-time PCR are rapid assays with results available in 24h. In the present study, we have successfully used a novel MGB TaqMan probe-based real time PCR assay for rapid diagnosis of trisomy 21 status in Down syndrome samples. We have also compared the results of this molecular method with corresponding results obtained by the cytogenetic analysis. Blood samples obtained from DS patients (n=25) and normal controls (n=20) were tested by quantitative Real-time PCR in parallel to standard G-banding analysis. Genomic DNA was extracted from peripheral blood lymphocytes. A high precision TaqMan probe quantitative Real-time PCR assay was developed to determine the gene dosage of DSCAM (target gene on 21q22.2) relative to PMP22 (reference gene on 17p11.2). The DSCAM/PMP22 ratio was calculated according to the formula; ratio=2 -ΔΔCT. The quantitative Real-time PCR was able to distinguish between trisomy 21 samples and normal controls with the gene ratios of 1.49±0.13 and 1.03±0.04 respectively (p value <0.001). These results represent the presence of 3 copies of target gene in DS samples Vs 2 copies in normal controls. The results of quantitative Real-time PCR were in complete agreement with results of cytogenetic analysis. This study confirms previous reports regarding successful implementation of quantitative Real-time PCR for detection of trisomy 21. However, the assay has been improved by using MGB probes and more accurate data analysis. This assay, in particular, when performed in combination with another molecular assay such as QF-PCR or MLPA, can be used as a reliable technique for rapid prenatal diagnosis of trisomy 21.</p> <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/search?q=Trisomy%2021" title="Trisomy 21">Trisomy 21</a>, <a href="https://publications.waset.org/search?q=Real-time%20PCR" title=" Real-time PCR"> Real-time PCR</a>, <a href="https://publications.waset.org/search?q=MGB-TaqMan%20Probes" title=" MGB-TaqMan Probes"> MGB-TaqMan Probes</a>, <a href="https://publications.waset.org/search?q=Gene%20Dosage." title="Gene Dosage.">Gene Dosage.</a> </p> <a href="https://publications.waset.org/15008/a-novel-multiplex-real-time-pcr-assay-using-taqman-mgb-probes-for-rapid-detection-of-trisomy-21" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/15008/apa" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">APA</a> <a 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