Clonal Dissemination of Pseudomonas aeruginosa Isolates in Kermanshah Hospitals, West of Iran

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publication-listing mt-3 mb-3"> <h5 class="card-header" style="font-size:.9rem">Clonal Dissemination of Pseudomonas aeruginosa Isolates in Kermanshah Hospitals, West of Iran</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Alisha%20Akya">Alisha Akya</a>, <a href="https://publications.waset.org/abstracts/search?q=Afsaneh%20salami"> Afsaneh salami</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Background and Objective: Pseudomonas aeruginosa is an opportunistic pathogen associated with nosocomial infections. One of the major concerns for the treatment of P. aeruginosa infections is its resistant to a variety of antibiotics. The purpose of this study was to assess the dissemination of p. aeruginosa isolates obtained from major hospitals in Kermanshah, west of Iran. Materials and Methods: Antibiotic susceptibility testing was performed using the minimal inhibitory concentrations. Mettalo-beta-lactamase was investigated using the double disk diffusion (DDST) test and PCR. Molecular typing was performed by pulsed-field gel electrophoresis (PFGE). Results: The 60 P. aeruginosa isolates, 30 (50%) were resistant to gentamicin, 38 (63/3%) to piperacilin, 42 (70%) to ceftazidime, and 45 (75%) to cefepime. Twenty-nine (48/3%) isolates were MBLs producer based on the DDST test. Five (8/3%) isolates were positive for VIM gene and 4 of them were from burn specimens. PFGE analysis among MBLs producers revealed 12 distinct genotype patterns. A pattern covering the highest number of strains was determined as the dominant clone. Conclusions: Our study showed that P. aeruginosa strains can be spread between patients in hospitals or acquired from different environmental sources. P. aeruginosa isolates were highly resistant to antibiotics and, therefore, the susceptibility of isolates to antibiotics should be tested before treatment. Given the clinical significance of MBLs producing isolates, identification of these organisms is essential in the hospitals in order to get a better therapeutic response and control of bacterial dissemination. <iframe src="https://publications.waset.org/abstracts/32371.pdf" style="width:100%; height:400px;" frameborder="0"></iframe> <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=clonal%20dissemination" title="clonal dissemination">clonal dissemination</a>, <a href="https://publications.waset.org/abstracts/search?q=mettalo-beta-lactamase" title=" mettalo-beta-lactamase"> mettalo-beta-lactamase</a>, <a href="https://publications.waset.org/abstracts/search?q=Pseudomonas%20aeruginosa" title=" Pseudomonas aeruginosa"> Pseudomonas aeruginosa</a>, <a href="https://publications.waset.org/abstracts/search?q=PFGE" title=" PFGE "> PFGE </a> </p> <a href="https://publications.waset.org/abstracts/32371/clonal-dissemination-of-pseudomonas-aeruginosa-isolates-in-kermanshah-hospitals-west-of-iran" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/32371.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">326</span> </span> </div> </div> </div> </main> <footer> <div id="infolinks" class="pt-3 pb-2"> <div class="container"> <div style="background-color:#f5f5f5;" class="p-3"> <div class="row"> <div class="col-md-2"> <ul class="list-unstyled"> About <li><a href="https://waset.org/page/support">About Us</a></li> <li><a href="https://waset.org/page/support#legal-information">Legal</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/WASET-16th-foundational-anniversary.pdf">WASET celebrates its 16th foundational anniversary</a></li> </ul> 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Clonal Dissemination of Pseudomonas aeruginosa Isolates in Kermanshah Hospitals, West of Iran