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.team-second-header { font-size: 40px !important; line-height: 50px !important; margin-top: 68px; margin-bottom: 20px; margin-left: 0; } .member-image { margin-left: 0; margin-bottom: 20px; } .member-description { margin-right: 0; } /* About us */ .about-content-col .intro-para p { font-size: 16px; line-height: 24px; } .about-content-col .intro-para h2 { font-size: 22px; line-height: 32px; } /* Product page */ .navinci-bg-left.vc_custom_1633423029422, .navinci-bg-left.vc_custom_1633423043835 { background-position: left !important; background-size: cover !important; } .navinci-bg-right.vc_custom_1633423010215, .navinci-bg-right.vc_custom_1633423017590 { background-position: right !important; background-size: cover !important; } .navinci-bg-left::before, .navinci-bg-right::before { content: ""; display: block; position: absolute; top: 0; right: 0; bottom: 0; left: 0; background-color: rgba(0,0,0,0.4); width: 100%; height: 100%; } } // ------- EVENTS PAGE -------- //21st CIMT row .clickable-row { box-shadow: rgba(0, 0, 0, 0) 0 0 0; } .clickable-row:hover { box-shadow: rgba(0, 0, 0, 0.16) 0 1px 5px; cursor: pointer; border-radius: 0px; box-shadow: rgba(0, 0, 0, 0.07) 0px 0px 0px 1px; } // -- PLA workshop at rudbeck row .workshop-rudbeck-row { box-shadow: rgba(0, 0, 0, 0) 0 0 0; } .workshop-rudbeck-row:hover { box-shadow: rgba(0, 0, 0, 0.16) 0 1px 5px; cursor: pointer; border-radius: 0px; box-shadow: rgba(0, 0, 0, 0.07) 0px 0px 0px 1px; } // ----- AACR 2024 row .aacr-2024-row { box-shadow: rgba(0, 0, 0, 0) 0 0 0; } .aacr-2024-row:hover { box-shadow: rgba(0, 0, 0, 0.16) 0 1px 5px; cursor: pointer; border-radius: 0px; box-shadow: rgba(0, 0, 0, 0.07) 0px 0px 0px 1px; } // ----- WEBINAR MARCH 26 row .webinar-march26 { box-shadow: rgba(0, 0, 0, 0) 0 0 0; } .webinar-march26:hover { box-shadow: rgba(0, 0, 0, 0.16) 0 1px 5px; cursor: pointer; border-radius: 0px; box-shadow: rgba(0, 0, 0, 0.07) 0px 0px 0px 1px; } // ----- ITOC-2024 row .itoc-2024 { box-shadow: rgba(0, 0, 0, 0) 0 0 0; } .itoc-2024:hover { box-shadow: rgba(0, 0, 0, 0.16) 0 1px 5px; cursor: pointer; border-radius: 0px; box-shadow: rgba(0, 0, 0, 0.07) 0px 0px 0px 1px; } // ----- EAI-2024 row .eai-2024 { box-shadow: rgba(0, 0, 0, 0) 0 0 0; } .eai-2024:hover { box-shadow: rgba(0, 0, 0, 0.16) 0 1px 5px; cursor: pointer; border-radius: 0px; box-shadow: rgba(0, 0, 0, 0.07) 0px 0px 0px 1px; } // ----- spatial biology east coast 2024 row .sbec-24 { box-shadow: rgba(0, 0, 0, 0) 0 0 0; } .sbec-24:hover { box-shadow: rgba(0, 0, 0, 0.16) 0 1px 5px; cursor: pointer; border-radius: 0px; box-shadow: rgba(0, 0, 0, 0.07) 0px 0px 0px 1px; } // ----- festival of genomics row .fgb-24 { box-shadow: rgba(0, 0, 0, 0) 0 0 0; } .fgb-24:hover { box-shadow: rgba(0, 0, 0, 0.16) 0 1px 5px; cursor: pointer; border-radius: 0px; box-shadow: rgba(0, 0, 0, 0.07) 0px 0px 0px 1px; } // ----- VIB Spatial Omics row .vib-so-24 { box-shadow: rgba(0, 0, 0, 0) 0 0 0; } .vib-so-24:hover { box-shadow: rgba(0, 0, 0, 0.16) 0 1px 5px; cursor: pointer; border-radius: 0px; box-shadow: rgba(0, 0, 0, 0.07) 0px 0px 0px 1px; } // ----- EACR row .eacr-24 { box-shadow: rgba(0, 0, 0, 0) 0 0 0; } .eacr-24:hover { box-shadow: rgba(0, 0, 0, 0.16) 0 1px 5px; cursor: pointer; border-radius: 0px; box-shadow: rgba(0, 0, 0, 0.07) 0px 0px 0px 1px; } // ----- milano row .milano-24 { box-shadow: rgba(0, 0, 0, 0) 0 0 0; } .milano-24:hover { box-shadow: rgba(0, 0, 0, 0.16) 0 1px 5px; cursor: pointer; border-radius: 0px; box-shadow: rgba(0, 0, 0, 0.07) 0px 0px 0px 1px; } // ----- milner row .milner-24 { box-shadow: rgba(0, 0, 0, 0) 0 0 0; } .milner-24:hover { box-shadow: rgba(0, 0, 0, 0.16) 0 1px 5px; cursor: pointer; border-radius: 0px; box-shadow: rgba(0, 0, 0, 0.07) 0px 0px 0px 1px; } // ----- mbc row .mbc-24 { box-shadow: rgba(0, 0, 0, 0) 0 0 0; } .mbc-24:hover { box-shadow: rgba(0, 0, 0, 0.16) 0 1px 5px; cursor: pointer; border-radius: 0px; box-shadow: rgba(0, 0, 0, 0.07) 0px 0px 0px 1px; } // ----- nsh row .nsh-24 { box-shadow: rgba(0, 0, 0, 0) 0 0 0; } .nsh-24:hover { box-shadow: rgba(0, 0, 0, 0.16) 0 1px 5px; cursor: pointer; border-radius: 0px; box-shadow: rgba(0, 0, 0, 0.07) 0px 0px 0px 1px; } // ----- ehf row .ehf-24 { box-shadow: rgba(0, 0, 0, 0) 0 0 0; } .ehf-24:hover { box-shadow: rgba(0, 0, 0, 0.16) 0 1px 5px; cursor: pointer; border-radius: 0px; box-shadow: rgba(0, 0, 0, 0.07) 0px 0px 0px 1px; } // ----- spatial europe row .spatialeurope-24 { box-shadow: rgba(0, 0, 0, 0) 0 0 0; } .spatialeurope-24:hover { box-shadow: rgba(0, 0, 0, 0.16) 0 1px 5px; cursor: pointer; border-radius: 0px; box-shadow: rgba(0, 0, 0, 0.07) 0px 0px 0px 1px; } // ----- spatial scilifelabs row .spatialscilifelabs-24 { box-shadow: rgba(0, 0, 0, 0) 0 0 0; } .spatialscilifelabs-24:hover { box-shadow: rgba(0, 0, 0, 0.16) 0 1px 5px; cursor: pointer; border-radius: 0px; box-shadow: rgba(0, 0, 0, 0.07) 0px 0px 0px 1px; } // ----- neuroscience row .neuroscience-24 { box-shadow: rgba(0, 0, 0, 0) 0 0 0; } .neuroscience-24:hover { 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class="wpb-content-wrapper"><div data-vc-full-width="true" data-vc-full-width-init="false" class="vc_row wpb_row vc_row-fluid vc_custom_1649426101939 vc_row-has-fill"><div class="wpb_column vc_column_container vc_col-sm-12"><div class="vc_column-inner vc_custom_1649425950030"><div class="wpb_wrapper"> <div class="wpb_text_column wpb_content_element " > <div class="wpb_wrapper"> <h1 style="text-align: center;"><span style="color: #ffffff;">The Naveni® <em>in situ</em> proximity ligation technology</span></h1> </div> </div> </div></div></div></div><div class="vc_row-full-width vc_clearfix"></div><section data-vc-full-width="true" data-vc-full-width-init="false" class="vc_section vc_custom_1689082543534 vc_section-has-fill vc_section-o-content-middle vc_section-flex"><div data-vc-full-width="true" data-vc-full-width-init="false" class="vc_row wpb_row vc_row-fluid vc_custom_1633696106461 vc_row-has-fill"><div class="wpb_column vc_column_container vc_col-sm-2"><div class="vc_column-inner"><div class="wpb_wrapper"></div></div></div><div class="wpb_column vc_column_container vc_col-sm-8"><div class="vc_column-inner"><div class="wpb_wrapper"><h2 style="color: #ffffff;text-align: left" class="vc_custom_heading vc_do_custom_heading" >Illuminating function in spatial proteomics</h2> <div class="wpb_text_column wpb_content_element " > <div class="wpb_wrapper"> <p><span style="color: #ffffff;"><span lang="EN-US" xml:lang="EN-US" data-contrast="auto">The Naveni<sup>®</sup> </span><em><span lang="EN-US" xml:lang="EN-US" data-contrast="auto">in situ </span></em><span lang="EN-US" xml:lang="EN-US" data-contrast="auto">proximity ligation technology </span><span lang="EN-US" xml:lang="EN-US" data-contrast="auto">pushes the boundaries of fluorescence-based </span><span lang="EN-US" xml:lang="EN-US" data-contrast="auto">in situ </span><span lang="EN-US" xml:lang="EN-US" data-contrast="auto">methodology, enabling researchers to get the maximum information from every analysis by visualizing and quantifying proteins, their interactions and modifications, </span><span lang="EN-US" xml:lang="EN-US" data-contrast="auto">in situ</span><span lang="EN-US" xml:lang="EN-US" data-contrast="auto"> at the molecular level. An ability to detect even low abundant proteins ensures that proteins can be investigated without the need to overexpress or modify in any way the natural environment of the cell. Data generated contributes to a deeper understanding of the biological mechanisms within diseased or normal tissues, responses to therapeutic drugs or changes to the cellular microenvironment. The Naveni<sup>®</sup> </span><em><span lang="EN-US" xml:lang="EN-US" data-contrast="auto">in situ</span></em><span lang="EN-US" xml:lang="EN-US" data-contrast="auto"> proximity ligation technology provides clean, highly resolved images, ready for quantification.</span> </span></p> </div> </div> </div></div></div><div class="wpb_column vc_column_container vc_col-sm-2"><div class="vc_column-inner"><div class="wpb_wrapper"></div></div></div></div><div class="vc_row-full-width vc_clearfix"></div><div data-vc-full-width="true" data-vc-full-width-init="false" class="vc_row wpb_row vc_row-fluid vc_custom_1633696169025 vc_row-has-fill"><div class="wpb_column vc_column_container vc_col-sm-2"><div class="vc_column-inner"><div class="wpb_wrapper"></div></div></div><div class="mob_mt_30 wpb_column vc_column_container vc_col-sm-4"><div class="vc_column-inner"><div class="wpb_wrapper"> <div class="wpb_text_column wpb_content_element " > <div class="wpb_wrapper"> <p><span style="color: #f4f4f4;"><strong>The benefits of our technology are:</strong></span></p> <ul> <li><span style="color: #f4f4f4;">Clear target detection</span></li> <li><span style="color: #f4f4f4;">Consistency in staining</span></li> <li><span style="color: #f4f4f4;">Reproducibility</span></li> <li><span style="color: #f4f4f4;">Possibility to detect extremely low abundant proteins</span></li> <li><span style="color: #f4f4f4;">Detection of protein-protein interactions</span></li> <li><span style="color: #f4f4f4;">Specific detection of post-translational modificatio</span><span style="color: #ffffff;"><span style="color: #f4f4f4;">ns, also with the use o</span>f pan-specific antibodies</span></li> </ul> </div> </div> </div></div></div><div class="wpb_column vc_column_container vc_col-sm-4"><div class="vc_column-inner"><div class="wpb_wrapper"> <div class="wpb_text_column wpb_content_element " > <div class="wpb_wrapper"> <p><span style="color: #f4f4f4;"><strong>Our technology is tailored for academic and industrial researchers working in fields of:</strong></span></p> <ul> <li><span style="color: #f4f4f4;">Immuno-profiling</span></li> <li><span style="color: #f4f4f4;">Signaling profiling</span></li> <li><span style="color: #f4f4f4;">Drug testing</span></li> <li><span style="color: #f4f4f4;">Treatment validation</span></li> <li><span style="color: #f4f4f4;">Biomarker discovery</span></li> <li><span style="color: #f4f4f4;">Diagnostics</span></li> </ul> </div> </div> </div></div></div><div class="wpb_column vc_column_container vc_col-sm-2"><div class="vc_column-inner"><div class="wpb_wrapper"></div></div></div></div><div class="vc_row-full-width vc_clearfix"></div></section><div class="vc_row-full-width vc_clearfix"></div><section data-vc-full-width="true" data-vc-full-width-init="false" class="vc_section vc_custom_1695119683992 vc_section-has-fill"><div class="vc_row wpb_row vc_row-fluid vc_custom_1625224368262"><div class="wpb_column vc_column_container vc_col-sm-2"><div class="vc_column-inner"><div class="wpb_wrapper"></div></div></div><div class="wpb_column vc_column_container vc_col-sm-8"><div class="vc_column-inner"><div class="wpb_wrapper"><h2 style="color: #c44809;text-align: left" class="vc_custom_heading vc_do_custom_heading" >Workflow</h2></div></div></div><div class="wpb_column vc_column_container vc_col-sm-2"><div class="vc_column-inner"><div class="wpb_wrapper"></div></div></div></div><div class="vc_row wpb_row vc_row-fluid vc_row-o-content-bottom vc_row-flex"><div class="wpb_column vc_column_container vc_col-sm-2 vc_hidden-sm vc_hidden-xs"><div class="vc_column-inner"><div class="wpb_wrapper"></div></div></div><div class="wpb_column vc_column_container vc_col-sm-2 vc_hidden-sm vc_hidden-xs"><div class="vc_column-inner"><div class="wpb_wrapper"> <div class="wpb_single_image wpb_content_element vc_align_left"> <figure class="wpb_wrapper vc_figure"> <div class="vc_single_image-wrapper vc_box_border_grey"><img fetchpriority="high" decoding="async" width="1024" height="1024" src="data:image/svg+xml,%3Csvg%20xmlns='http://www.w3.org/2000/svg'%20viewBox='0%200%201024%201024'%3E%3C/svg%3E" class="vc_single_image-img attachment-large" alt="" title="new_workflow-01" data-dt-location="https://navinci.se/new_workflow-01/" data-lazy-src="https://navinci.se/wp-content/uploads/2023/01/new_workflow-01.svg" /><noscript><img fetchpriority="high" decoding="async" width="1024" height="1024" src="https://navinci.se/wp-content/uploads/2023/01/new_workflow-01.svg" class="vc_single_image-img attachment-large" alt="" title="new_workflow-01" data-dt-location="https://navinci.se/new_workflow-01/" /></noscript></div> </figure> </div> </div></div></div><div class="wpb_column vc_column_container vc_col-sm-2 vc_hidden-sm vc_hidden-xs"><div class="vc_column-inner"><div class="wpb_wrapper"> <div class="wpb_single_image wpb_content_element vc_align_left"> <figure class="wpb_wrapper vc_figure"> <div class="vc_single_image-wrapper vc_box_border_grey"><img decoding="async" width="1024" height="1024" src="data:image/svg+xml,%3Csvg%20xmlns='http://www.w3.org/2000/svg'%20viewBox='0%200%201024%201024'%3E%3C/svg%3E" class="vc_single_image-img attachment-large" alt="" title="new_workflow-03" data-dt-location="https://navinci.se/new_workflow-03/" data-lazy-src="https://navinci.se/wp-content/uploads/2023/01/new_workflow-03.svg" /><noscript><img decoding="async" width="1024" height="1024" src="https://navinci.se/wp-content/uploads/2023/01/new_workflow-03.svg" class="vc_single_image-img attachment-large" alt="" title="new_workflow-03" data-dt-location="https://navinci.se/new_workflow-03/" /></noscript></div> </figure> </div> </div></div></div><div class="wpb_column vc_column_container vc_col-sm-2 vc_hidden-sm vc_hidden-xs"><div class="vc_column-inner"><div class="wpb_wrapper"> <div class="wpb_single_image wpb_content_element vc_align_left"> <figure class="wpb_wrapper vc_figure"> <div class="vc_single_image-wrapper vc_box_border_grey"><img decoding="async" width="1024" height="1024" src="data:image/svg+xml,%3Csvg%20xmlns='http://www.w3.org/2000/svg'%20viewBox='0%200%201024%201024'%3E%3C/svg%3E" class="vc_single_image-img attachment-large" alt="" title="new_workflow-04" data-dt-location="https://navinci.se/new_workflow-04/" data-lazy-src="https://navinci.se/wp-content/uploads/2023/01/new_workflow-04.svg" /><noscript><img decoding="async" width="1024" height="1024" src="https://navinci.se/wp-content/uploads/2023/01/new_workflow-04.svg" class="vc_single_image-img attachment-large" alt="" title="new_workflow-04" data-dt-location="https://navinci.se/new_workflow-04/" /></noscript></div> </figure> </div> </div></div></div><div class="wpb_column vc_column_container vc_col-sm-2 vc_hidden-sm vc_hidden-xs"><div class="vc_column-inner"><div class="wpb_wrapper"> <div class="wpb_single_image wpb_content_element vc_align_left"> <figure class="wpb_wrapper vc_figure"> <div class="vc_single_image-wrapper vc_box_border_grey"><img decoding="async" width="1024" height="1024" src="data:image/svg+xml,%3Csvg%20xmlns='http://www.w3.org/2000/svg'%20viewBox='0%200%201024%201024'%3E%3C/svg%3E" class="vc_single_image-img attachment-large" alt="" title="new_workflow-06" data-dt-location="https://navinci.se/new_workflow-06/" data-lazy-src="https://navinci.se/wp-content/uploads/2023/01/new_workflow-06.svg" /><noscript><img decoding="async" width="1024" height="1024" src="https://navinci.se/wp-content/uploads/2023/01/new_workflow-06.svg" class="vc_single_image-img attachment-large" alt="" title="new_workflow-06" data-dt-location="https://navinci.se/new_workflow-06/" /></noscript></div> </figure> </div> </div></div></div><div class="wpb_column vc_column_container vc_col-sm-2"><div class="vc_column-inner"><div class="wpb_wrapper"></div></div></div></div><div class="vc_row wpb_row vc_row-fluid vc_row-o-content-top vc_row-flex"><div class="wpb_column vc_column_container vc_col-sm-2 vc_hidden-sm vc_hidden-xs"><div class="vc_column-inner"><div class="wpb_wrapper"></div></div></div><div class="wpb_column vc_column_container vc_col-sm-2 vc_hidden-sm vc_hidden-xs"><div class="vc_column-inner"><div class="wpb_wrapper"> <div class="wpb_text_column wpb_content_element all_sec_fz_14" > <div class="wpb_wrapper"> <p style="text-align: center;"><strong>Primary antibody incubation</strong></p> <p style="text-align: center;">Two primary antibodies bind to their target epitopes, located on a single protein or two nearby proteins.</p> </div> </div> </div></div></div><div class="wpb_column vc_column_container vc_col-sm-2 vc_hidden-sm vc_hidden-xs"><div class="vc_column-inner"><div class="wpb_wrapper"> <div class="wpb_text_column wpb_content_element all_sec_fz_14" > <div class="wpb_wrapper"> <p style="text-align: center;"><strong>Navenibody incubation</strong></p> <p style="text-align: center;">Navenibodies (carefully selected antibodies conjugated to proprietary oligonucleotide arms) bind their respective primary antibodies.</p> </div> </div> </div></div></div><div class="wpb_column vc_column_container vc_col-sm-2 vc_hidden-sm vc_hidden-xs"><div class="vc_column-inner"><div class="wpb_wrapper"> <div class="wpb_text_column wpb_content_element all_sec_fz_14" > <div class="wpb_wrapper"> <p style="text-align: center;"><strong>Circle formation</strong></p> <p style="text-align: center;">Only if the Navenibodies are in close proximity the attached oligos can generate a DNA circle guaranteeing high specificity by reducing nonspecific background staining.</p> <p style="text-align: center;">Using the proprietary Naveni<sup>®</sup> technology, the efficacy of the oligo design improves the signal strength, ensuring high sensitivity.</p> </div> </div> </div></div></div><div class="wpb_column vc_column_container vc_col-sm-2"><div class="vc_column-inner"><div class="wpb_wrapper"> <div class="wpb_text_column wpb_content_element all_sec_fz_14" > <div class="wpb_wrapper"> <p style="text-align: center;"><strong>Amplification and detection</strong></p> <p style="text-align: center;">By the addition of polymerase, a rolling circle amplification process is initiated. Fluorescent or HRP/AP labeled probes are bound to the amplified DNA, generating a fluorescence or a chromogenic dot after adding substrate.</p> <p style="text-align: center;">The high signal-to-noise enables the detection of separate proximity events, allowing for a resolution down to a single protein or protein-protein interaction.</p> </div> </div> </div></div></div><div class="wpb_column vc_column_container vc_col-sm-2"><div class="vc_column-inner"><div class="wpb_wrapper"></div></div></div></div><div data-vc-full-width="true" data-vc-full-width-init="false" class="vc_row wpb_row vc_row-fluid vc_custom_1679665922388 vc_row-has-fill"><div class="wpb_column vc_column_container vc_col-sm-12 vc_hidden-lg vc_hidden-md"><div class="vc_column-inner"><div class="wpb_wrapper"> <div class="wpb_single_image wpb_content_element vc_align_center"> <figure class="wpb_wrapper vc_figure"> <div class="vc_single_image-wrapper vc_box_border_grey"><img decoding="async" data-vc-zoom="https://navinci.se/wp-content/uploads/2022/12/NaveniFlex_NaveniFlex-lab-workflow.svg" width="1024" height="1024" src="data:image/svg+xml,%3Csvg%20xmlns='http://www.w3.org/2000/svg'%20viewBox='0%200%201024%201024'%3E%3C/svg%3E" class="vc_single_image-img attachment-large" alt="" title="NaveniFlex_NaveniFlex lab workflow" data-dt-location="https://navinci.se/naveniflex_naveniflex-lab-workflow/" data-lazy-src="https://navinci.se/wp-content/uploads/2022/12/NaveniFlex_NaveniFlex-lab-workflow.svg" /><noscript><img decoding="async" data-vc-zoom="https://navinci.se/wp-content/uploads/2022/12/NaveniFlex_NaveniFlex-lab-workflow.svg" width="1024" height="1024" src="https://navinci.se/wp-content/uploads/2022/12/NaveniFlex_NaveniFlex-lab-workflow.svg" class="vc_single_image-img attachment-large" alt="" title="NaveniFlex_NaveniFlex lab workflow" data-dt-location="https://navinci.se/naveniflex_naveniflex-lab-workflow/" /></noscript></div> </figure> </div> <div class="vc_empty_space" style="height: 20px"><span class="vc_empty_space_inner"></span></div> <div class="wpb_text_column wpb_content_element para-small" > <div class="wpb_wrapper"> <p><strong>Primary antibody incubation: </strong>Two primary antibodies bind to their target epitopes, located on a single protein or two nearby proteins. <strong>Navenibody incubation: </strong>Navenibodies (carefully selected antibodies conjugated to proprietary oligonucleotide arms) bind their respective primary antibodies. <strong>Circle formation: </strong>Only if the Navenibodies are in close proximity the attached oligos can generate a DNA circle guaranteeing high specificity by reducing nonspecific background staining. Using the proprietary Naveni<sup>TM</sup> technology, the efficacy of the oligo design improves the signal strength, ensuring high sensitivity. <strong>Amplification and detection: </strong>By the addition of polymerase, a rolling circle amplification process is initiated. Fluorescent or HRP/AP labeled probes are bound to the amplified DNA, generating a fluorescence or a chromogenic dot after adding substrate. The high signal-to-noise enables the detection of separate proximity events, allowing for a resolution down to a single protein or protein-protein interaction.</p> </div> </div> </div></div></div></div><div class="vc_row-full-width vc_clearfix"></div><div data-vc-full-width="true" data-vc-full-width-init="false" class="vc_row wpb_row vc_row-fluid vc_custom_1679665836396 vc_row-has-fill"><div class="wpb_column vc_column_container vc_col-sm-12 vc_hidden-lg vc_hidden-md vc_hidden-sm vc_hidden-xs"><div class="vc_column-inner"><div class="wpb_wrapper"><h2 style="color: #c44809;text-align: left" class="vc_custom_heading vc_do_custom_heading vc_custom_1689083266714" >Proximity ligation: A revolutionary protein detection technology - Made in Uppsala</h2> <div class="wpb_text_column wpb_content_element " id="ProximityLigationAssayPlatform"> <div class="wpb_wrapper"> <p style="font-weight: 400;"><b><strong>Cellular processes can only be understood as the dynamic interplay of molecules. This means that we need accurate techniques to monitor interactions of endogenous proteins directly in individual cells and tissues, to reveal the cellular and molecular architecture and its responses to disturbances.</strong></b></p> <p style="font-weight: 400;"><b><strong> </strong></b>Protein detection with higher sensitivity and specificity is a constant need in any research area involving proteins. Also, we need the possibility to study how proteins interact, and how they are modified and expressed in their proper tissue microenvironment. Ulf Landegren’s research group, at the Immunology, Genetics and Pathology Department at Uppsala University, decided to develop a method that could provide a solution to meet these growing needs in proteomics. In a seminal paper by Simon Fredriksson et al., 2002, the technology termed proximity ligation was first described. In the paper the authors showed that highly specific and sensitive protein detection could be achieved by complementing two DNA aptamer-probes with sequence extensions that could be joined by ligation. In this manner probes that bind in close proximity by recognizing the same target molecule could be joined to form a template for detection via sensitive real-time PCR amplification. In subsequent work by the group, the aptamers were replaced as affinity reagents by oligonucleotide-conjugated antibodies, serving as proximity probes. The application of proximity assays for detecting proteins in solution phase evolved into proximity extension assays, that are commercialized as high-performance multiplex assays by Olink Proteomics, spun out from Olink Bioscience in 2016.</p> <p style="font-weight: 400;">Ola Söderberg et al. first described the application of the <em>in situ </em>proximity ligation technology for localized protein detection in 2006. The authors used the method for localizing proteins and their interactions in fixed cells or tissues, by combining proximity ligation with rolling circle amplification (RCA) for localized readout <em>in situ</em>. When pairs of proximity probes bind protein targets in close proximity their conjugated oligonucleotides can template circularization of an added pair of oligonucleotides via enzymatic ligation. The circularized DNA strands remain hybridized to the proximity probes and one of the oligonucleotides serves as a primer for a localized RCA reaction. The resulting amplified DNA can then be detected as brightly fluorescent spots by suitably labeled detection probes.</p> <p style="font-weight: 400;"> The paper demonstrated the ability of the proximity ligation technology to detect the intra- and intercellular distribution of endogenous proteins at the single-molecule level, which is very useful when studying biological processes such as regulation of proliferation, differentiation and survival in complex microenvironments for example in tumors. Because samples are fixed before analysis, the technology provides snapshots of cellular processes, including both stable and transient interactions, at single cell and subcellular resolution within the undisturbed microenvironment. This patented in situ proximity ligation technology was commercialized under the name Duolink by Olink Biosciences and has been used in over 7500 peer-reviewed publications globally. The Duolink product line was licensed to Sigma Aldrich in 2016.</p> <p style="font-weight: 400;"> In 2018, Axel Klaesson et al., published a paper describing a new proximity probe design called UnFold, based on new, patented oligonucleotide sequences. The UnFold technique differs from the earlier version of the <em>in situ</em> PLA technique in that all DNA elements required for amplified detection are included in the oligonucleotides conjugated to the antibodies. (See Workflow above).</p> <p style="font-weight: 400;"> Compared to the conventional <em>in situ</em> PLA probe design, the UnFold <em>in situ</em> proximity probes improve the efficiency of signal generation: The oligonucleotide that can form a circle is already present on one of the probes, and only one ligation reaction is required, which increases the likelihood that circles can form that are capable of serving as template for RCA. The UnFold technique is commercialized under the trademark Naveni<sup>TM</sup>Flex by Navinci, which is the new name of Olink Biosciences.</p> <p style="font-weight: 400;"> <em>In situ</em> proximity ligation has been applied to improve sensitivity, specificity, and target range in many methods for localized protein detection besides for microscopy, for example, western blotting, flow cytometry and sandwich enzyme-linked immunosorbent assays (ELISA). This technology is positioned to play an integral part in spatial biology research and clinical applications, for high-performance protein detection in situ, and to study protein-protein interactions and post-translational modifications directly in the intact tissue microenvironment.</p> </div> </div> </div></div></div></div><div class="vc_row-full-width vc_clearfix"></div></section><div class="vc_row-full-width vc_clearfix"></div> </div> </div> </div> </div> </div>  <footer id="footer" class="footer solid-bg"> <div class="wf-wrap"> <div class="wf-container-footer"> <div class="wf-container"> <section id="custom_html-2" class="widget_text widget widget_custom_html wf-cell wf-1-2"><div class="widget-title">Contact us</div><div class="textwidget custom-html-widget"><p>Email: <a href="mailto:contact@navinci.se">contact@navinci.se</a></p></div></section><section id="nav_menu-2" class="widget widget_nav_menu wf-cell wf-1-2"><div class="widget-title">Menu</div><div class="menu-footern-container"><ul id="menu-footern" class="menu"><li id="menu-item-9270" class="menu-item menu-item-type-post_type menu-item-object-page menu-item-9270"><a href="https://navinci.se/products/">Products</a></li> <li id="menu-item-9282" class="menu-item menu-item-type-custom menu-item-object-custom current-menu-item menu-item-9282"><a href="https://navinci.se/technology/" aria-current="page">Technology</a></li> <li id="menu-item-9283" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-9283"><a href="https://navinci.se/applications/">Applications</a></li> <li id="menu-item-9284" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-9284"><a href="https://navinci.se/support/">Support</a></li> <li id="menu-item-9285" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-9285"><a href="https://navinci.se/about/">About</a></li> </ul></div></section> </div> </div> </div>  <div id="bottom-bar" class="logo-left" role="contentinfo"> <div class="wf-wrap"> <div class="wf-container-bottom"> <div class="wf-float-right"> <div class="bottom-text-block"><p><a class="elementor-icon" href="https://www.linkedin.com/company/navinci-diagnostics/"><i class="fa fa-linkedin-square" aria-hidden="true"></i></a></p> </div> </div> </div> </div> </div> </footer> <a href="#" class="scroll-top"><svg version="1.1" id="Layer_1" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" x="0px" y="0px" viewBox="0 0 16 16" style="enable-background:new 0 0 16 16;" xml:space="preserve"> <path d="M11.7,6.3l-3-3C8.5,3.1,8.3,3,8,3c0,0,0,0,0,0C7.7,3,7.5,3.1,7.3,3.3l-3,3c-0.4,0.4-0.4,1,0,1.4c0.4,0.4,1,0.4,1.4,0L7,6.4 V12c0,0.6,0.4,1,1,1s1-0.4,1-1V6.4l1.3,1.3c0.4,0.4,1,0.4,1.4,0C11.9,7.5,12,7.3,12,7S11.9,6.5,11.7,6.3z"/> </svg><span class="screen-reader-text">Go to Top</span></a> </div> <script> window.RS_MODULES = window.RS_MODULES || {}; 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