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This method relies upon the use of proteins called restriction enzymes, which can cut, or digest, DNA molecules at short, specific sequences called restriction sites. After a DNA segment has been digested using a restriction enzyme, the resulting fragments can be examined using a laboratory method called gel electrophoresis, which is used to separate pieces of DNA according to their size. </p> <p> </p> <p>One common method for constructing a restriction map involves digesting the unknown DNA sample in three ways. Here, two portions of the DNA sample are individually digested with different restriction enzymes, and a third portion of the DNA sample is double-digested with both restriction enzymes at the same time. Next, each digestion sample is separated using gel electrophoresis, and the sizes of the DNA fragments are recorded. The total length of the fragments in each digestion will be equal. However, because the length of each individual DNA fragment depends upon the positions of its restriction sites, each restriction site can be mapped according to the lengths of the fragments. The information from the double-digestion is particularly useful for correctly mapping the sites. The final drawing of the DNA segment that shows the positions of the restriction sites is called a restriction map.</p> </div> </div> <div class="clear"></div> </div> </div> <div class="clear"></div> </div> </div> <div class="articleFurtherReadingList"> <hr class="courseHR"/> <p class="bigH1">Further Exploration</p> <div id=""> <p class="nomargin px16 marginbot5px">Concept Links for further exploration</p> <div class="px16"> <a class="inlineLinks px16 normalFontWeight" href="/scitable/definition/mapping-282">mapping</a> | <a class="inlineLinks px16 normalFontWeight" href="/scitable/definition/dna-107">DNA</a> | <a class="inlineLinks px16 normalFontWeight" href="/scitable/definition/gel-electrophoresis-286">gel electrophoresis</a> | <a class="inlineLinks px16 normalFontWeight" href="/scitable/definition/restriction-endonuclease-144">restriction endonuclease</a> | <a class="inlineLinks px16 normalFontWeight" href="/scitable/definition/rflp-285">RFLP</a> </div> </div> </div> </div> </div> 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{ /*$("#header").html($("#headerOuter").html()) ; $("#headerOuter").html('');*/ var str = document.getElementById("headerOuter").innerHTML; document.getElementById("headerOuter").innerHTML = ""; document.getElementById("header").innerHTML = str; if(document.getElementById("site-new-header-section")){ document.getElementById("site-new-header-section").innerHTML = document.getElementById("siteNewHeaderOuter").innerHTML; document.getElementById("site-new-header-section").style.display = 'block'; } // document.getElementById(). } catch(e) { } //}); placeBool = false; var signINReqMsgNonLoggedIN = "Scitable requires you to be signed in to use this feature. If you are not a member of Scitable, registration is free and easy. As a registered user you can have access to all content in Scitable, interact directly with community members, create a personal locker, start and join groups and discussions, and more"; var signINReqMsgNonLoggedINToolTip = "You have to be logged in to use this feature."; var collapseLabelPage = "View Fewer"; </script> <script type="text/plain" data-cc-script="C04"> var isTopicLandingPageFllg = ''; var topicID = ''; var invToJoinGrpGrpID = ""; var currentPagerItem = 1; </script> <script data-cc-script="C04" data-src="/scitable/nated/natedjs/paginationUtil.js?1098" type="text/javascript"></script> <script data-cc-script="C04" data-src="/scitable/nated/natedjs/articleCommon.js?1098" type="text/javascript"></script> <script data-cc-script="C04" data-src="/scitable/nated/natedjs/swfobject.js?1098" type="text/javascript"></script> <script type="text/plain" data-cc-script="C04"> var ontologyPK = "283"; var browserIs = "OTH"; var contentPK = "NA"; var cleanUrlContextPath = "/scitable"; var cleanBaseURL = '/scitable'; function ontologyPageLoader(){ $(document).ready(function(){ searchPageNav = 'showSearchPageOntology'; refreshVideos(); loadUserProfileWidgets(); tabInit(); //loadOntologyNodeRelatedContentsData(ontologyPK); loadMiniVBScreen(); }); } function detach(clickedContentPKInner){ //var url= parent.baseURL + 'browse?action=displayVisualBrowse&topicPK=' + topicPK + '&detach=true&contentPK=' + contentPKInner + '&type=' + clickedNodeTypeInner + '&urlString=' + escape(urlString) + '&rootPk=' + rootPk + '&rootType=' + rootType + '&groupInfo=true&groupUrlString='+escape(groupUrlString); var url= baseURL + 'browse?action=displayVisualBrowse&detach=true&contentPK=' + ontologyPK + '&type=ontology'; //alert(url); detachOntologyScreenVB(url); } function detachOntologyScreenVB(url) { var detachedWindow; //alert("Here :" +url); if(browserIs!= "IE"){ var browser = navigator.userAgent; if(browser.indexOf("Safari")>-1){ window.open(url, "VisualBrowse", "height=630, width=780, address=no, status=no, scrollbars=no, resizable=1"); } else{ window.open(url, "VisualBrowse", "height=610, width=770, resizable=1, address=no, status=no"); } } else{ window.open(url, "VisualBrowse", "height=610, width=760, resizable=0, address=no, status=no"); } } function loadUserProfileWidgets(){ widgetList = new Array(); widgetList.push("loadOnlineUsers"); widgetList.push("loadNumMessages"); loadPageWidgets(widgetList); } function loadOntologyNodeRelatedContentsData(ontologyNodePK){ url = baseURL + 'upload?action=viewOntologyContent&newSearch=true&pager_userSearchName=ontologyContent&pager_servlet=upload&ontologyNodePK=' + ontologyNodePK; AjaxUtil.request(url, { onComplete: function(xmlHttpReq) { var msg = xmlHttpReq.responseText; if(msg == 'false') { // alert('Msg is false.'); // document.getElementById('ontologyRelatedContentLabel').className = "hide"; document.getElementById('ontologyContent').className = "hide"; } else { // alert('We have data...'); // document.getElementById('ontologyRelatedContentLabel').className = "dispblock"; document.getElementById('ontologyContent').className = "dispblock"; document.getElementById('ontologyContent').innerHTML = msg; } } }); } function showSearchPageOntology(msg,formID){ if(msg!=null) document.getElementById('ontologyContent').innerHTML = msg; } function showHideOntologyFromSearch(toggleKey){ url = baseURL + 'upload?action=updateOntologySearchFlag&ajaxFlag=true&ontologyNodePK=' + ontologyPK + '&toggleKey=' + toggleKey; AjaxUtil.request(url, { onComplete: function(xmlHttpReq) { var msg = xmlHttpReq.responseText; if(msg == 'true'){ if(toggleKey == 'Y'){ document.getElementById('ontologyToggleSpanHdr').innerHTML = "<a onClick='showHideOntologyFromSearch(\"N\");return false;' class='inlineLinks' href='#'>Hide</a>"; } else { document.getElementById('ontologyToggleSpanHdr').innerHTML = "<a onClick='showHideOntologyFromSearch(\"Y\");return false;' class='inlineLinks' href='#'>Searchable</a>"; } } else { show_inform("Ontology Page", "Error occurs."); } } }); } </script> <script type="text/plain" data-cc-script="C04"> var gaJsHost = (("https:" == document.location.protocol) ? "https://ssl." : "http://www."); (function (s) { s.src = gaJsHost + 'google-analytics.com/ga.js'; s.type = 'text/javascript'; document.head.appendChild(s); })(document.createElement('script')); </script> <script type="text/plain" data-cc-script="C04"> try { var pageTracker = _gat._getTracker("UA-12063924-2"); pageTracker._trackPageview(); } catch(err) {}</script> </div> </body> </html>

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