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Benet","profile_url":"https://independent.academia.edu/JordiSalbanyaBenet?f_ri=956315","photo":"https://0.academia-photos.com/5847665/3124316/15964805/s65_jordi_salbanya.benet.jpeg"}],"research_interests":[{"id":7700,"name":"Fluorescence Microscopy","url":"https://www.academia.edu/Documents/in/Fluorescence_Microscopy?f_ri=956315","nofollow":false},{"id":65882,"name":"Fluorescence in situ hybridization","url":"https://www.academia.edu/Documents/in/Fluorescence_in_situ_hybridization?f_ri=956315","nofollow":false},{"id":138959,"name":"Aneuploidy","url":"https://www.academia.edu/Documents/in/Aneuploidy?f_ri=956315","nofollow":false},{"id":351108,"name":"Human reproduction","url":"https://www.academia.edu/Documents/in/Human_reproduction?f_ri=956315","nofollow":false},{"id":376291,"name":"Fluorescent in situ hybridization","url":"https://www.academia.edu/Documents/in/Fluorescent_in_situ_hybridization?f_ri=956315"},{"id":683802,"name":"Polar Body","url":"https://www.academia.edu/Documents/in/Polar_Body?f_ri=956315"},{"id":956315,"name":"Oocytes","url":"https://www.academia.edu/Documents/in/Oocytes?f_ri=956315"},{"id":1147230,"name":"Chromosomes","url":"https://www.academia.edu/Documents/in/Chromosomes?f_ri=956315"},{"id":1575801,"name":"DNA probes","url":"https://www.academia.edu/Documents/in/DNA_probes?f_ri=956315"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_24433039" data-work_id="24433039" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/24433039/Chromatin_decondensation_pronucleus_formation_metaphase_entry_and_chromosome_complements_of_human_spermatozoa_after_intracytoplasmic_sperm_injection_into_hamster_oocytes">Chromatin decondensation, pronucleus formation, metaphase entry and chromosome complements of human spermatozoa after intracytoplasmic sperm injection into hamster oocytes</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/24433039" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="48252fbaad623b44a801716ca84b7c08" rel="nofollow" data-download="{"attachment_id":44762623,"asset_id":24433039,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button 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href="https://www.academia.edu/Documents/in/Intracytoplasmic_Sperm_Injection">Intracytoplasmic Sperm Injection</a>, <script data-card-contents-for-ri="98929" type="text/json">{"id":98929,"name":"Intracytoplasmic Sperm Injection","url":"https://www.academia.edu/Documents/in/Intracytoplasmic_Sperm_Injection?f_ri=956315","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="153639" href="https://www.academia.edu/Documents/in/Karyotyping">Karyotyping</a><script data-card-contents-for-ri="153639" type="text/json">{"id":153639,"name":"Karyotyping","url":"https://www.academia.edu/Documents/in/Karyotyping?f_ri=956315","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=24433039]'), work: {"id":24433039,"title":"Chromatin decondensation, pronucleus formation, metaphase entry and chromosome complements of human spermatozoa after intracytoplasmic sperm injection into hamster oocytes","created_at":"2016-04-15T06:48:18.068-07:00","url":"https://www.academia.edu/24433039/Chromatin_decondensation_pronucleus_formation_metaphase_entry_and_chromosome_complements_of_human_spermatozoa_after_intracytoplasmic_sperm_injection_into_hamster_oocytes?f_ri=956315","dom_id":"work_24433039","summary":null,"downloadable_attachments":[{"id":44762623,"asset_id":24433039,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":47098804,"first_name":"Marc","last_name":"Dhont","domain_name":"independent","page_name":"MarcDhont","display_name":"Marc Dhont","profile_url":"https://independent.academia.edu/MarcDhont?f_ri=956315","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=956315","nofollow":false},{"id":65133,"name":"Parthenogenesis","url":"https://www.academia.edu/Documents/in/Parthenogenesis?f_ri=956315","nofollow":false},{"id":98929,"name":"Intracytoplasmic Sperm Injection","url":"https://www.academia.edu/Documents/in/Intracytoplasmic_Sperm_Injection?f_ri=956315","nofollow":false},{"id":153639,"name":"Karyotyping","url":"https://www.academia.edu/Documents/in/Karyotyping?f_ri=956315","nofollow":false},{"id":178928,"name":"Male Infertility","url":"https://www.academia.edu/Documents/in/Male_Infertility?f_ri=956315"},{"id":238813,"name":"Chromatin","url":"https://www.academia.edu/Documents/in/Chromatin?f_ri=956315"},{"id":291842,"name":"Human Spermatozoa","url":"https://www.academia.edu/Documents/in/Human_Spermatozoa?f_ri=956315"},{"id":317801,"name":"Cell nucleus","url":"https://www.academia.edu/Documents/in/Cell_nucleus?f_ri=956315"},{"id":351108,"name":"Human reproduction","url":"https://www.academia.edu/Documents/in/Human_reproduction?f_ri=956315"},{"id":613442,"name":"Fertilization in Vitro","url":"https://www.academia.edu/Documents/in/Fertilization_in_Vitro?f_ri=956315"},{"id":862371,"name":"Spermatozoa","url":"https://www.academia.edu/Documents/in/Spermatozoa?f_ri=956315"},{"id":956315,"name":"Oocytes","url":"https://www.academia.edu/Documents/in/Oocytes?f_ri=956315"},{"id":957359,"name":"Culture Media","url":"https://www.academia.edu/Documents/in/Culture_Media?f_ri=956315"},{"id":1166930,"name":"Cytoplasm","url":"https://www.academia.edu/Documents/in/Cytoplasm?f_ri=956315"},{"id":2111545,"name":"Spontaneous Activity","url":"https://www.academia.edu/Documents/in/Spontaneous_Activity?f_ri=956315"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_24001195" data-work_id="24001195" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/24001195/Cyclin_B1_expression_in_meiotically_competent_and_incompetent_goat_oocytes">Cyclin B1 expression in meiotically competent and incompetent goat oocytes</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/24001195" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="2df7088647238409b1a5caf66abbc6a7" rel="nofollow" data-download="{"attachment_id":44386198,"asset_id":24001195,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/44386198/download_file?st=MTczMjc2NjI2Miw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="38171063" href="https://independent.academia.edu/IsabelleHue">Isabelle Hue</a><script data-card-contents-for-user="38171063" type="text/json">{"id":38171063,"first_name":"Isabelle","last_name":"Hue","domain_name":"independent","page_name":"IsabelleHue","display_name":"Isabelle Hue","profile_url":"https://independent.academia.edu/IsabelleHue?f_ri=956315","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_24001195 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="24001195"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 24001195, container: ".js-paper-rank-work_24001195", }); });</script></li><li class="js-percentile-work_24001195 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 24001195; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_24001195"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_24001195 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="24001195"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 24001195; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=24001195]").text(description); $(".js-view-count-work_24001195").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_24001195").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="24001195"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i> <a class="InlineList-item-text u-positionRelative">11</a> </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="37836" href="https://www.academia.edu/Documents/in/In_Situ_Hybridization">In Situ Hybridization</a>, <script data-card-contents-for-ri="37836" type="text/json">{"id":37836,"name":"In Situ Hybridization","url":"https://www.academia.edu/Documents/in/In_Situ_Hybridization?f_ri=956315","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="73564" href="https://www.academia.edu/Documents/in/Meiosis">Meiosis</a>, <script data-card-contents-for-ri="73564" type="text/json">{"id":73564,"name":"Meiosis","url":"https://www.academia.edu/Documents/in/Meiosis?f_ri=956315","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="118339" href="https://www.academia.edu/Documents/in/Polymerase_Chain_Reaction">Polymerase Chain Reaction</a>, <script data-card-contents-for-ri="118339" type="text/json">{"id":118339,"name":"Polymerase Chain Reaction","url":"https://www.academia.edu/Documents/in/Polymerase_Chain_Reaction?f_ri=956315","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="252969" href="https://www.academia.edu/Documents/in/Goats">Goats</a><script data-card-contents-for-ri="252969" type="text/json">{"id":252969,"name":"Goats","url":"https://www.academia.edu/Documents/in/Goats?f_ri=956315","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=24001195]'), work: {"id":24001195,"title":"Cyclin B1 expression in meiotically competent and incompetent goat oocytes","created_at":"2016-04-04T03:58:44.211-07:00","url":"https://www.academia.edu/24001195/Cyclin_B1_expression_in_meiotically_competent_and_incompetent_goat_oocytes?f_ri=956315","dom_id":"work_24001195","summary":null,"downloadable_attachments":[{"id":44386198,"asset_id":24001195,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":38171063,"first_name":"Isabelle","last_name":"Hue","domain_name":"independent","page_name":"IsabelleHue","display_name":"Isabelle Hue","profile_url":"https://independent.academia.edu/IsabelleHue?f_ri=956315","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":37836,"name":"In Situ Hybridization","url":"https://www.academia.edu/Documents/in/In_Situ_Hybridization?f_ri=956315","nofollow":false},{"id":73564,"name":"Meiosis","url":"https://www.academia.edu/Documents/in/Meiosis?f_ri=956315","nofollow":false},{"id":118339,"name":"Polymerase Chain Reaction","url":"https://www.academia.edu/Documents/in/Polymerase_Chain_Reaction?f_ri=956315","nofollow":false},{"id":252969,"name":"Goats","url":"https://www.academia.edu/Documents/in/Goats?f_ri=956315","nofollow":false},{"id":260829,"name":"Cattle","url":"https://www.academia.edu/Documents/in/Cattle?f_ri=956315"},{"id":809881,"name":"Amino Acid Sequence","url":"https://www.academia.edu/Documents/in/Amino_Acid_Sequence?f_ri=956315"},{"id":809882,"name":"Base Sequence","url":"https://www.academia.edu/Documents/in/Base_Sequence?f_ri=956315"},{"id":956315,"name":"Oocytes","url":"https://www.academia.edu/Documents/in/Oocytes?f_ri=956315"},{"id":1116087,"name":"Granulosa Cells","url":"https://www.academia.edu/Documents/in/Granulosa_Cells?f_ri=956315"},{"id":1681026,"name":"Biochemistry and cell biology","url":"https://www.academia.edu/Documents/in/Biochemistry_and_cell_biology?f_ri=956315"},{"id":1763968,"name":"Gene Expression Regulation","url":"https://www.academia.edu/Documents/in/Gene_Expression_Regulation?f_ri=956315"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_66336288" data-work_id="66336288" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/66336288/Effect_of_Polyvinylpyrrolidone_on_Vitrification_of_Buffalo_Bubalus_bubalis_Oocytes">Effect of Polyvinylpyrrolidone on Vitrification of Buffalo (Bubalus bubalis) Oocytes</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Vitrification, a method of rapid cooling, is an alternate cryopreservation method of oocytes and embryos. The present study was aimed to examine the effect of polyvinylpyrrolidone (PVP) on vitrification of buffalo oocytes. Cumulus oocyte... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_66336288" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Vitrification, a method of rapid cooling, is an alternate cryopreservation method of oocytes and embryos. The present study was aimed to examine the effect of polyvinylpyrrolidone (PVP) on vitrification of buffalo oocytes. Cumulus oocyte complexes (COCs) with fully grown oocytes (120-130 µm in diameter) were aspirated from slaughtered buffalo ovaries for vitrification. COCs were treated with equilibration solution at room temperature for 5 min and then transferred to a vitrification solution for 1 min. Then the COCs were submerged into liquid nitrogen (-196C) for a while using cryotops. The COCs were thawed, diluted, and washed in a washing solution for 5 min, respectively. Vitrified oocytes were incubated for in vitro maturation (IVM) at 38.5C under an atmosphere of 5% CO2 in the air for 24 hrs. Cumulus cells surrounding the oocytes were removed mechanically, oocytes were fixed in acetic acid and ethanol, and stained with aceto-orcein to examine the meiotic stages of oocytes. The numbers of morphologically normal oocytes after vitrification were higher in 5% PVP than 0 and 10% PVP groups. A proportion of oocytes treated with 5% PVP reached the metaphase II (MII) stage while none of the oocytes from 0% and 10% PVP groupsdeveloped beyond anaphase I and metaphase I (MI) stages, respectively. These results show that PVP can be used as a cryoprotectant for the vitrification of buffalo oocytes.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/66336288" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="501563a05893d6c081d3eaa421a73e64" rel="nofollow" data-download="{"attachment_id":77569374,"asset_id":66336288,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/77569374/download_file?st=MTczMjc2NjI2Miw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="211349963" href="https://independent.academia.edu/lifescienceglobal">Lifescience Global Canada</a><script data-card-contents-for-user="211349963" type="text/json">{"id":211349963,"first_name":"Lifescience","last_name":"Global Canada","domain_name":"independent","page_name":"lifescienceglobal","display_name":"Lifescience Global Canada","profile_url":"https://independent.academia.edu/lifescienceglobal?f_ri=956315","photo":"https://0.academia-photos.com/211349963/71416544/59860660/s65_lifescience.global_canada.jpg"}</script></span></span></li><li class="js-paper-rank-work_66336288 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="66336288"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 66336288, container: ".js-paper-rank-work_66336288", }); 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The present study was aimed to examine the effect of polyvinylpyrrolidone (PVP) on vitrification of buffalo oocytes. Cumulus oocyte complexes (COCs) with fully grown oocytes (120-130 µm in diameter) were aspirated from slaughtered buffalo ovaries for vitrification. COCs were treated with equilibration solution at room temperature for 5 min and then transferred to a vitrification solution for 1 min. Then the COCs were submerged into liquid nitrogen (-196C) for a while using cryotops. The COCs were thawed, diluted, and washed in a washing solution for 5 min, respectively. Vitrified oocytes were incubated for in vitro maturation (IVM) at 38.5C under an atmosphere of 5% CO2 in the air for 24 hrs. Cumulus cells surrounding the oocytes were removed mechanically, oocytes were fixed in acetic acid and ethanol, and stained with aceto-orcein to examine the meiotic stages of oocytes. The numbers of morphologically normal oocytes after vitrification were higher in 5% PVP than 0 and 10% PVP groups. A proportion of oocytes treated with 5% PVP reached the metaphase II (MII) stage while none of the oocytes from 0% and 10% PVP groupsdeveloped beyond anaphase I and metaphase I (MI) stages, respectively. These results show that PVP can be used as a cryoprotectant for the vitrification of buffalo oocytes.","downloadable_attachments":[{"id":77569374,"asset_id":66336288,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":211349963,"first_name":"Lifescience","last_name":"Global Canada","domain_name":"independent","page_name":"lifescienceglobal","display_name":"Lifescience Global 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Development","url":"https://www.academia.edu/Documents/in/Embryonic_Development?f_ri=956315"},{"id":613442,"name":"Fertilization in Vitro","url":"https://www.academia.edu/Documents/in/Fertilization_in_Vitro?f_ri=956315"},{"id":956026,"name":"Somatic Cell Count","url":"https://www.academia.edu/Documents/in/Somatic_Cell_Count?f_ri=956315"},{"id":956315,"name":"Oocytes","url":"https://www.academia.edu/Documents/in/Oocytes?f_ri=956315"},{"id":1147230,"name":"Chromosomes","url":"https://www.academia.edu/Documents/in/Chromosomes?f_ri=956315"},{"id":1799766,"name":"Biology Reproduction","url":"https://www.academia.edu/Documents/in/Biology_Reproduction?f_ri=956315"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_24447087" data-work_id="24447087" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/24447087/The_effects_of_brief_gamete_co_incubation_in_human_in_vitro_fertilization">The effects of brief gamete co-incubation in human in vitro fertilization</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">To investigate whether very short exposure of mature oocytes to sperm in vitro may affect the fertilization rates, embryo cleavage rates, and embryo quality between sibling oocytes in the same patient. Sibling oocytes of the same patient... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_24447087" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">To investigate whether very short exposure of mature oocytes to sperm in vitro may affect the fertilization rates, embryo cleavage rates, and embryo quality between sibling oocytes in the same patient. Sibling oocytes of the same patient from 23 oocyte collection cycles were randomly allocated to the study group, with a 1-hr or 3-hr sperm-oocyte incubation, or the control group with the standard overnight gamete co-incubation. The fertilization rates, cleavage rates, and subsequent embryo quality were evaluated. Our results showed no statistically significant differences in fertilization rates, embryo cleavage rates, and quality of the embryos between the study group and the control group. Since the present study showed that long exposure of the oocyte to sperm has no advantage over short exposure, we prefer shortening the oocyte-sperm incubation period for reducing the negative effect induced by nonphysiologically high concentrations of spermatozoa.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/24447087" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="0c1b2f0665216e69a8a72d1ad6be8d53" rel="nofollow" data-download="{"attachment_id":44778177,"asset_id":24447087,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/44778177/download_file?st=MTczMjc2NjI2Miw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="47127101" href="https://independent.academia.edu/RobertKuoKuangLee">Robert Kuo-Kuang Lee</a><script data-card-contents-for-user="47127101" type="text/json">{"id":47127101,"first_name":"Robert Kuo-Kuang","last_name":"Lee","domain_name":"independent","page_name":"RobertKuoKuangLee","display_name":"Robert Kuo-Kuang Lee","profile_url":"https://independent.academia.edu/RobertKuoKuangLee?f_ri=956315","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_24447087 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="24447087"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 24447087, container: ".js-paper-rank-work_24447087", }); 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data-has-card-for-ri="156" href="https://www.academia.edu/Documents/in/Genetics">Genetics</a>, <script data-card-contents-for-ri="156" type="text/json">{"id":156,"name":"Genetics","url":"https://www.academia.edu/Documents/in/Genetics?f_ri=956315","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="8562" href="https://www.academia.edu/Documents/in/Informed_Consent">Informed Consent</a>, <script data-card-contents-for-ri="8562" type="text/json">{"id":8562,"name":"Informed Consent","url":"https://www.academia.edu/Documents/in/Informed_Consent?f_ri=956315","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="19295" href="https://www.academia.edu/Documents/in/Cryopreservation">Cryopreservation</a>, <script data-card-contents-for-ri="19295" type="text/json">{"id":19295,"name":"Cryopreservation","url":"https://www.academia.edu/Documents/in/Cryopreservation?f_ri=956315","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="62112" href="https://www.academia.edu/Documents/in/Prospective_studies">Prospective studies</a><script data-card-contents-for-ri="62112" type="text/json">{"id":62112,"name":"Prospective studies","url":"https://www.academia.edu/Documents/in/Prospective_studies?f_ri=956315","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=17763916]'), work: {"id":17763916,"title":"Application of intra- and extracellular sugars and dimethylsulfoxide to human oocyte 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Consent","url":"https://www.academia.edu/Documents/in/Informed_Consent?f_ri=956315","nofollow":false},{"id":19295,"name":"Cryopreservation","url":"https://www.academia.edu/Documents/in/Cryopreservation?f_ri=956315","nofollow":false},{"id":62112,"name":"Prospective studies","url":"https://www.academia.edu/Documents/in/Prospective_studies?f_ri=956315","nofollow":false},{"id":78754,"name":"Cancer Therapy","url":"https://www.academia.edu/Documents/in/Cancer_Therapy?f_ri=956315"},{"id":165001,"name":"Trehalose","url":"https://www.academia.edu/Documents/in/Trehalose?f_ri=956315"},{"id":424295,"name":"Survival Rate","url":"https://www.academia.edu/Documents/in/Survival_Rate?f_ri=956315"},{"id":956315,"name":"Oocytes","url":"https://www.academia.edu/Documents/in/Oocytes?f_ri=956315"},{"id":1027717,"name":"Embryos","url":"https://www.academia.edu/Documents/in/Embryos?f_ri=956315"},{"id":1281442,"name":"Extracellular Space","url":"https://www.academia.edu/Documents/in/Extracellular_Space?f_ri=956315"},{"id":1311259,"name":"Intracellular Space","url":"https://www.academia.edu/Documents/in/Intracellular_Space?f_ri=956315"},{"id":1631043,"name":"Control Group","url":"https://www.academia.edu/Documents/in/Control_Group?f_ri=956315"},{"id":1863536,"name":"Assisted","url":"https://www.academia.edu/Documents/in/Assisted?f_ri=956315"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_20390628" data-work_id="20390628" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/20390628/Predictive_value_of_oocyte_morphology_in_human_IVF_a_systematic_review_of_the_literature">Predictive value of oocyte morphology in human IVF: a systematic review of the literature</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/20390628" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="cbdc316ddbd3542296e5fb634c95d556" rel="nofollow" data-download="{"attachment_id":41333834,"asset_id":20390628,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" 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u-positionRelative">7</a> </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="19295" href="https://www.academia.edu/Documents/in/Cryopreservation">Cryopreservation</a>, <script data-card-contents-for-ri="19295" type="text/json">{"id":19295,"name":"Cryopreservation","url":"https://www.academia.edu/Documents/in/Cryopreservation?f_ri=956315","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="221411" href="https://www.academia.edu/Documents/in/Systematic_review">Systematic review</a>, <script data-card-contents-for-ri="221411" type="text/json">{"id":221411,"name":"Systematic review","url":"https://www.academia.edu/Documents/in/Systematic_review?f_ri=956315","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="244814" href="https://www.academia.edu/Documents/in/Clinical_Sciences">Clinical Sciences</a>, <script data-card-contents-for-ri="244814" type="text/json">{"id":244814,"name":"Clinical Sciences","url":"https://www.academia.edu/Documents/in/Clinical_Sciences?f_ri=956315","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="410370" href="https://www.academia.edu/Documents/in/Public_health_systems_and_services_research-1">Public health systems and services research</a><script data-card-contents-for-ri="410370" type="text/json">{"id":410370,"name":"Public health systems and services research","url":"https://www.academia.edu/Documents/in/Public_health_systems_and_services_research-1?f_ri=956315","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=20390628]'), work: {"id":20390628,"title":"Predictive value of oocyte morphology in human IVF: a systematic review of the 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href="https://www.academia.edu/72101285/Interaction_of_human_spermatozoa_with_the_zona_pellucida_of_oocyte_development_of_the_acrosome_reaction">Interaction of human spermatozoa with the zona pellucida of oocyte: development of the acrosome reaction</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/72101285" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="87c22937b4c8066e373536ee841b95ff" rel="nofollow" data-download="{"attachment_id":81166739,"asset_id":72101285,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/81166739/download_file?st=MTczMjc2NjI2Miw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="42341405" href="https://independent.academia.edu/MiguelLlanos6">Miguel Llanos</a><script data-card-contents-for-user="42341405" type="text/json">{"id":42341405,"first_name":"Miguel","last_name":"Llanos","domain_name":"independent","page_name":"MiguelLlanos6","display_name":"Miguel Llanos","profile_url":"https://independent.academia.edu/MiguelLlanos6?f_ri=956315","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_72101285 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="72101285"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 72101285, container: ".js-paper-rank-work_72101285", }); });</script></li><li class="js-percentile-work_72101285 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 72101285; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_72101285"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_72101285 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="72101285"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 72101285; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=72101285]").text(description); $(".js-view-count-work_72101285").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_72101285").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="72101285"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i> <a class="InlineList-item-text u-positionRelative">8</a> </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="523" href="https://www.academia.edu/Documents/in/Chemistry">Chemistry</a>, <script data-card-contents-for-ri="523" type="text/json">{"id":523,"name":"Chemistry","url":"https://www.academia.edu/Documents/in/Chemistry?f_ri=956315","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="26327" href="https://www.academia.edu/Documents/in/Medicine">Medicine</a>, <script data-card-contents-for-ri="26327" type="text/json">{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine?f_ri=956315","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="862371" href="https://www.academia.edu/Documents/in/Spermatozoa">Spermatozoa</a>, <script data-card-contents-for-ri="862371" type="text/json">{"id":862371,"name":"Spermatozoa","url":"https://www.academia.edu/Documents/in/Spermatozoa?f_ri=956315","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="956315" href="https://www.academia.edu/Documents/in/Oocytes">Oocytes</a><script data-card-contents-for-ri="956315" type="text/json">{"id":956315,"name":"Oocytes","url":"https://www.academia.edu/Documents/in/Oocytes?f_ri=956315","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=72101285]'), work: {"id":72101285,"title":"Interaction of human spermatozoa with the zona pellucida of oocyte: development of the acrosome reaction","created_at":"2022-02-21T06:30:58.441-08:00","url":"https://www.academia.edu/72101285/Interaction_of_human_spermatozoa_with_the_zona_pellucida_of_oocyte_development_of_the_acrosome_reaction?f_ri=956315","dom_id":"work_72101285","summary":null,"downloadable_attachments":[{"id":81166739,"asset_id":72101285,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":42341405,"first_name":"Miguel","last_name":"Llanos","domain_name":"independent","page_name":"MiguelLlanos6","display_name":"Miguel Llanos","profile_url":"https://independent.academia.edu/MiguelLlanos6?f_ri=956315","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":523,"name":"Chemistry","url":"https://www.academia.edu/Documents/in/Chemistry?f_ri=956315","nofollow":false},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine?f_ri=956315","nofollow":false},{"id":862371,"name":"Spermatozoa","url":"https://www.academia.edu/Documents/in/Spermatozoa?f_ri=956315","nofollow":false},{"id":956315,"name":"Oocytes","url":"https://www.academia.edu/Documents/in/Oocytes?f_ri=956315","nofollow":false},{"id":956824,"name":"Zona Pellucida","url":"https://www.academia.edu/Documents/in/Zona_Pellucida?f_ri=956315"},{"id":1116087,"name":"Granulosa Cells","url":"https://www.academia.edu/Documents/in/Granulosa_Cells?f_ri=956315"},{"id":1219924,"name":"Acrosome Reaction","url":"https://www.academia.edu/Documents/in/Acrosome_Reaction?f_ri=956315"},{"id":1681026,"name":"Biochemistry and cell biology","url":"https://www.academia.edu/Documents/in/Biochemistry_and_cell_biology?f_ri=956315"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_69066353" data-work_id="69066353" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/69066353/Follicular_Fluid_redox_involvement_for_ovarian_follicle_growth">Follicular Fluid redox involvement for ovarian follicle growth</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">As the human ovarian follicle enlarges in the course of a regular cycle or following controlled ovarian stimulation, the changes in its structure reveal the oocyte environment composed of cumulus oophorus cells and the follicular fluid... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_69066353" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">As the human ovarian follicle enlarges in the course of a regular cycle or following controlled ovarian stimulation, the changes in its structure reveal the oocyte environment composed of cumulus oophorus cells and the follicular fluid (FF).In contrast to the dynamic nature of cells, the fluid compartment appears as a reservoir rich in biomolecules. In some aspects, it is similar to the plasma, but it also exhibits differences that likely relate to its specific localization around the oocyte. The chemical composition indicates that the follicular fluid is able to detect and buffer excessive amounts of reactive oxygen species, employing a variety of antioxidants, some of them components of the intracellular milieu.An important part is played by albumin through specific cysteine residues. But the fluid contains other molecules whose cysteine residues may be involved in sensing and buffering the local oxidative conditions. How these molecules are recruited and regulated to intervene su...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/69066353" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="7e0eea9464fb71d23c14c4c364d7106d" rel="nofollow" data-download="{"attachment_id":79306760,"asset_id":69066353,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/79306760/download_file?st=MTczMjc2NjI2Miw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="9668201" href="https://independent.academia.edu/LilianaMatos">Liliana Matos</a><script data-card-contents-for-user="9668201" type="text/json">{"id":9668201,"first_name":"Liliana","last_name":"Matos","domain_name":"independent","page_name":"LilianaMatos","display_name":"Liliana Matos","profile_url":"https://independent.academia.edu/LilianaMatos?f_ri=956315","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_69066353 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="69066353"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 69066353, container: ".js-paper-rank-work_69066353", }); 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In some aspects, it is similar to the plasma, but it also exhibits differences that likely relate to its specific localization around the oocyte. The chemical composition indicates that the follicular fluid is able to detect and buffer excessive amounts of reactive oxygen species, employing a variety of antioxidants, some of them components of the intracellular milieu.An important part is played by albumin through specific cysteine residues. But the fluid contains other molecules whose cysteine residues may be involved in sensing and buffering the local oxidative conditions. How these molecules are recruited and regulated to intervene su...","downloadable_attachments":[{"id":79306760,"asset_id":69066353,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":9668201,"first_name":"Liliana","last_name":"Matos","domain_name":"independent","page_name":"LilianaMatos","display_name":"Liliana Matos","profile_url":"https://independent.academia.edu/LilianaMatos?f_ri=956315","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":523,"name":"Chemistry","url":"https://www.academia.edu/Documents/in/Chemistry?f_ri=956315","nofollow":false},{"id":14292,"name":"Oxidative Stress","url":"https://www.academia.edu/Documents/in/Oxidative_Stress?f_ri=956315","nofollow":false},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine?f_ri=956315","nofollow":false},{"id":29290,"name":"Oogenesis","url":"https://www.academia.edu/Documents/in/Oogenesis?f_ri=956315","nofollow":false},{"id":29974,"name":"Disease susceptibility","url":"https://www.academia.edu/Documents/in/Disease_susceptibility?f_ri=956315"},{"id":51711,"name":"Antioxidants","url":"https://www.academia.edu/Documents/in/Antioxidants?f_ri=956315"},{"id":306957,"name":"Ovarian Follicle","url":"https://www.academia.edu/Documents/in/Ovarian_Follicle?f_ri=956315"},{"id":956315,"name":"Oocytes","url":"https://www.academia.edu/Documents/in/Oocytes?f_ri=956315"},{"id":1212104,"name":"Cellular Microenvironment","url":"https://www.academia.edu/Documents/in/Cellular_Microenvironment?f_ri=956315"},{"id":1256747,"name":"Oxidation-Reduction","url":"https://www.academia.edu/Documents/in/Oxidation-Reduction?f_ri=956315"},{"id":1258326,"name":"Follicular Fluid","url":"https://www.academia.edu/Documents/in/Follicular_Fluid?f_ri=956315"},{"id":1352490,"name":"Oxidants","url":"https://www.academia.edu/Documents/in/Oxidants?f_ri=956315"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_60831549" data-work_id="60831549" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/60831549/Cloning_Adult_Farm_Animals_A_Review_of_the_Possibilities_and_Problems_Associated_with_Somatic_Cell_Nuclear_Transfer">Cloning Adult Farm Animals: A Review of the Possibilities and Problems Associated with Somatic Cell Nuclear Transfer</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/60831549" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="00b3e14860752fc01ec8c24cbb17a2da" rel="nofollow" data-download="{"attachment_id":74103102,"asset_id":60831549,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/74103102/download_file?st=MTczMjc2NjI2Miw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="11940676" href="https://independent.academia.edu/MatthewWelborn">Matthew Welborn</a><script data-card-contents-for-user="11940676" type="text/json">{"id":11940676,"first_name":"Matthew","last_name":"Welborn","domain_name":"independent","page_name":"MatthewWelborn","display_name":"Matthew Welborn","profile_url":"https://independent.academia.edu/MatthewWelborn?f_ri=956315","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_60831549 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="60831549"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 60831549, container: ".js-paper-rank-work_60831549", }); 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A quantitative analysis of oocyte ovulation</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/58236267" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="7784c1c582bbf60ce4bee6c04cf38676" rel="nofollow" data-download="{"attachment_id":72746120,"asset_id":58236267,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/72746120/download_file?st=MTczMjc2NjI2Miw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="42233109" href="https://independent.academia.edu/VassieWare">Vassie Ware</a><script data-card-contents-for-user="42233109" type="text/json">{"id":42233109,"first_name":"Vassie","last_name":"Ware","domain_name":"independent","page_name":"VassieWare","display_name":"Vassie Ware","profile_url":"https://independent.academia.edu/VassieWare?f_ri=956315","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_58236267 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="58236267"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 58236267, container: ".js-paper-rank-work_58236267", }); 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Oocytes were cultured in medium supplemented with 0, 2000, 4000 or 8000 ng... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_48281984" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">The aim of this study was to evaluate the effect of progesterone supplementation and stage of oestrous cycle on in vitro maturation (IVM) of canine oocytes. Oocytes were cultured in medium supplemented with 0, 2000, 4000 or 8000 ng progesterone ml(-1) (Expt 1; n=274 oocytes) or 0, 20, 200 or 2000 ng progesterone ml(-1) (Expt 2; n=789 oocytes). In Expt 3, oocytes (n=1202) were cultured in a bi-phasic system of meiotic arrest followed by IVM, both in the presence of 0, 20, 200 or 2000 ng progesterone ml(-1). Rates of meiotic resumption for Expt 1 ranged from 40.0% to 58.5%; there were no significant differences among groups. In Expt 2, rate of meiotic resumption was significantly lower in the 2000 ng progesterone ml(-1) treatment (35.5%) compared with the 200 ng progesterone ml(-1) treatment (54.0%; P&lt;0.05). There were no significant differences in rates of maturation to metaphase II among treatments in Expt 1 (1.8-8.6%) or Expt 2 (8.4-14.7%); however, oocytes collected from ovarie...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/48281984" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="a1c8dbe847be870484255fce9eb59d2f" rel="nofollow" data-download="{"attachment_id":66977016,"asset_id":48281984,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/66977016/download_file?st=MTczMjc2NjI2Miw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="43178679" href="https://independent.academia.edu/DuaneKraemer">Duane Kraemer</a><script data-card-contents-for-user="43178679" type="text/json">{"id":43178679,"first_name":"Duane","last_name":"Kraemer","domain_name":"independent","page_name":"DuaneKraemer","display_name":"Duane Kraemer","profile_url":"https://independent.academia.edu/DuaneKraemer?f_ri=956315","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_48281984 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="48281984"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 48281984, container: ".js-paper-rank-work_48281984", }); 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Oocytes were cultured in medium supplemented with 0, 2000, 4000 or 8000 ng progesterone ml(-1) (Expt 1; n=274 oocytes) or 0, 20, 200 or 2000 ng progesterone ml(-1) (Expt 2; n=789 oocytes). In Expt 3, oocytes (n=1202) were cultured in a bi-phasic system of meiotic arrest followed by IVM, both in the presence of 0, 20, 200 or 2000 ng progesterone ml(-1). Rates of meiotic resumption for Expt 1 ranged from 40.0% to 58.5%; there were no significant differences among groups. In Expt 2, rate of meiotic resumption was significantly lower in the 2000 ng progesterone ml(-1) treatment (35.5%) compared with the 200 ng progesterone ml(-1) treatment (54.0%; P\u0026lt;0.05). 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href="https://www.academia.edu/6624503/Formalin_Induced_Short_and_Long_Term_Modulation_of_Ca_v_Currents_Expressed_in_Xenopus_Oocytes_An_In_Vitro_Cellular_Model_for_Formalin_Induced_Pain">Formalin-Induced Short and Long-Term Modulation of Ca v Currents Expressed in Xenopus Oocytes: An In Vitro Cellular Model for Formalin-Induced Pain</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Abstract: Xenopus oocytes expressing high voltage-gated calcium channels (Cav) were exposed to formalin (0.5%, v/v, 5 min.) and the oocyte death and Cav currents were studied for up to 10 days. Cav channels were expressed with α1β1b and... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_6624503" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Abstract: Xenopus oocytes expressing high voltage-gated calcium channels (Cav) were exposed to formalin (0.5%, v/v, 5 min.) and the oocyte death and Cav currents were studied for up to 10 days. Cav channels were expressed with α1β1b and α2δ sub-units and the currents (IBa) were studied by voltage clamp. None of the oocytes was dead during the exposure to formalin. Oocyte death was significant between day 1 and day 5 after the exposure to formalin and was uniform among the oocytes expressing various Cav channels. Peak IBa of all Cav and A1, the inactivating current component was decreased whereas the non-inactivated R current was not affected by 5 min. exposure to formalin. On day 1 after the exposure to formalin, Cav1.2c currents were increased, 2.1 and 2.2 currents were decreased and 2.3 currents were unaltered. On day 5, both peak IBa and A1 currents were increased. Cav1.2c, 2.2 and 2.3 currents were increased and Cav2.1 was unaltered on day 10 after the exposure to formalin. Protein kinase C (PKC) may be involved in formalin-induced increase in Cav currents due to the (i) requirement for Cavβ1b sub-units; (ii) decreased phorbol-12-myristate,13-acetate potentiation of Cav2.3 currents; (iii) absence of potentiation of Cav2.3 currents following down-regulation of PKC; and (iv) absence of potentiation of Cav2.2 or 2.3 currents with Ser→Ala mutation of Cavα12.2 or 2.3 sub-units. Increased Cav currents and PKC activation may coincide with changes observed in in vivo pain investigations, and oocytes incubated with formalin may serve as an in vitro model for some cellular mechanisms of pain.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/6624503" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="66e950c58c9218d4f7e174076ed8f95a" rel="nofollow" data-download="{"attachment_id":48779068,"asset_id":6624503,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/48779068/download_file?st=MTczMjc2NjI2Miw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="2418305" href="https://revauniversity.academia.edu/SenthilkumarRajagopal">Senthilkumar Rajagopal</a><script data-card-contents-for-user="2418305" type="text/json">{"id":2418305,"first_name":"Senthilkumar","last_name":"Rajagopal","domain_name":"revauniversity","page_name":"SenthilkumarRajagopal","display_name":"Senthilkumar Rajagopal","profile_url":"https://revauniversity.academia.edu/SenthilkumarRajagopal?f_ri=956315","photo":"https://0.academia-photos.com/2418305/89145077/77853870/s65_senthilkumar.rajagopal.jpg"}</script></span></span></li><li class="js-paper-rank-work_6624503 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="6624503"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 6624503, container: ".js-paper-rank-work_6624503", }); 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$(".js-view-count[data-work-id=6624503]").text(description); $(".js-view-count-work_6624503").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_6624503").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="6624503"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i> <a class="InlineList-item-text u-positionRelative">16</a> </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="2898" href="https://www.academia.edu/Documents/in/Pain">Pain</a>, <script data-card-contents-for-ri="2898" type="text/json">{"id":2898,"name":"Pain","url":"https://www.academia.edu/Documents/in/Pain?f_ri=956315","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="24925" href="https://www.academia.edu/Documents/in/Modulation">Modulation</a>, <script data-card-contents-for-ri="24925" type="text/json">{"id":24925,"name":"Modulation","url":"https://www.academia.edu/Documents/in/Modulation?f_ri=956315","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="37793" href="https://www.academia.edu/Documents/in/Patch-clamp_and_imaging_techniques">Patch-clamp and imaging techniques</a>, <script data-card-contents-for-ri="37793" type="text/json">{"id":37793,"name":"Patch-clamp and imaging techniques","url":"https://www.academia.edu/Documents/in/Patch-clamp_and_imaging_techniques?f_ri=956315","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="59370" href="https://www.academia.edu/Documents/in/In_Vitro">In Vitro</a><script data-card-contents-for-ri="59370" type="text/json">{"id":59370,"name":"In Vitro","url":"https://www.academia.edu/Documents/in/In_Vitro?f_ri=956315","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=6624503]'), work: {"id":6624503,"title":"Formalin-Induced Short and Long-Term Modulation of Ca v Currents Expressed in Xenopus Oocytes: An In Vitro Cellular Model for Formalin-Induced Pain","created_at":"2014-04-01T21:46:23.760-07:00","url":"https://www.academia.edu/6624503/Formalin_Induced_Short_and_Long_Term_Modulation_of_Ca_v_Currents_Expressed_in_Xenopus_Oocytes_An_In_Vitro_Cellular_Model_for_Formalin_Induced_Pain?f_ri=956315","dom_id":"work_6624503","summary":"Abstract: Xenopus oocytes expressing high voltage-gated calcium channels (Cav) were exposed to formalin (0.5%, v/v, 5 min.) and the oocyte death and Cav currents were studied for up to 10 days. Cav channels were expressed with α1β1b and α2δ sub-units and the currents (IBa) were studied by voltage clamp. None of the oocytes was dead during the exposure to formalin. Oocyte death was significant between day 1 and day 5 after the exposure to formalin and was uniform among the oocytes expressing various Cav channels. Peak IBa of all Cav and A1, the inactivating current component was decreased whereas the non-inactivated R current was not affected by 5 min. exposure to formalin. On day 1 after the exposure to formalin, Cav1.2c currents were increased, 2.1 and 2.2 currents were decreased and 2.3 currents were unaltered. On day 5, both peak IBa and A1 currents were increased. Cav1.2c, 2.2 and 2.3 currents were increased and Cav2.1 was unaltered on day 10 after the exposure to formalin. Protein kinase C (PKC) may be involved in formalin-induced increase in Cav currents due to the (i) requirement for Cavβ1b sub-units; (ii) decreased phorbol-12-myristate,13-acetate potentiation of Cav2.3 currents; (iii) absence of potentiation of Cav2.3 currents following down-regulation of PKC; and (iv) absence of potentiation of Cav2.2 or 2.3 currents with Ser→Ala mutation of Cavα12.2 or 2.3 sub-units. Increased Cav currents and PKC activation may coincide with changes observed in in vivo pain investigations, and oocytes incubated with formalin may serve as an in vitro model for some cellular mechanisms of pain.","downloadable_attachments":[{"id":48779068,"asset_id":6624503,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":2418305,"first_name":"Senthilkumar","last_name":"Rajagopal","domain_name":"revauniversity","page_name":"SenthilkumarRajagopal","display_name":"Senthilkumar Rajagopal","profile_url":"https://revauniversity.academia.edu/SenthilkumarRajagopal?f_ri=956315","photo":"https://0.academia-photos.com/2418305/89145077/77853870/s65_senthilkumar.rajagopal.jpg"}],"research_interests":[{"id":2898,"name":"Pain","url":"https://www.academia.edu/Documents/in/Pain?f_ri=956315","nofollow":false},{"id":24925,"name":"Modulation","url":"https://www.academia.edu/Documents/in/Modulation?f_ri=956315","nofollow":false},{"id":37793,"name":"Patch-clamp and imaging techniques","url":"https://www.academia.edu/Documents/in/Patch-clamp_and_imaging_techniques?f_ri=956315","nofollow":false},{"id":59370,"name":"In Vitro","url":"https://www.academia.edu/Documents/in/In_Vitro?f_ri=956315","nofollow":false},{"id":65140,"name":"Models","url":"https://www.academia.edu/Documents/in/Models?f_ri=956315"},{"id":99271,"name":"Calcium channels","url":"https://www.academia.edu/Documents/in/Calcium_channels?f_ri=956315"},{"id":102448,"name":"Basic Clinical Neurology","url":"https://www.academia.edu/Documents/in/Basic_Clinical_Neurology?f_ri=956315"},{"id":112576,"name":"Cell Death","url":"https://www.academia.edu/Documents/in/Cell_Death?f_ri=956315"},{"id":375054,"name":"Rats","url":"https://www.academia.edu/Documents/in/Rats?f_ri=956315"},{"id":413195,"name":"Time Factors","url":"https://www.academia.edu/Documents/in/Time_Factors?f_ri=956315"},{"id":477264,"name":"Xenopus laevis","url":"https://www.academia.edu/Documents/in/Xenopus_laevis?f_ri=956315"},{"id":819116,"name":"Formaldehyde","url":"https://www.academia.edu/Documents/in/Formaldehyde?f_ri=956315"},{"id":908385,"name":"Long Term","url":"https://www.academia.edu/Documents/in/Long_Term?f_ri=956315"},{"id":956315,"name":"Oocytes","url":"https://www.academia.edu/Documents/in/Oocytes?f_ri=956315"},{"id":1293238,"name":"Oocyte","url":"https://www.academia.edu/Documents/in/Oocyte?f_ri=956315"},{"id":1364640,"name":"Short Term","url":"https://www.academia.edu/Documents/in/Short_Term?f_ri=956315"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_6868905" data-work_id="6868905" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/6868905/The_Influence_of_Powdered_Coconut_Water_ACP318_in_In_Vitro_Maturation_of_Canine_Oocytes">The Influence of Powdered Coconut Water (ACP318 ® ) in In Vitro Maturation of Canine Oocytes</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">The objective of this study was to determine the influence of powdered coconut water (ACP-318®) diluted in high glucose (11.0 mm) TCM199 in the achievement of nuclear in vitro maturation (IVM) of canine oocytes. Cumulus oocyte complexes... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_6868905" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">The objective of this study was to determine the influence of powdered coconut water (ACP-318®) diluted in high glucose (11.0 mm) TCM199 in the achievement of nuclear in vitro maturation (IVM) of canine oocytes. Cumulus oocyte complexes (COCs) (n = 632) were randomly allocated into three experimental groups named as group 1 (control group), group 2 (5% powdered coconut water) and group 3 (10% powdered coconut water). The percentage of meiotic resumption (MR) (GVBD to MII) was 39.1% (81/207), 50.2% (108/215) and 46.6% (98/210) for groups 1, 2 and 3 respectively (p < 0.05). There were no differences in MR rates among groups 2 and 3. The medium with ACP-318® slightly enhanced the nuclear maturation of canine oocytes when a comparison was established with rates of maturation exhibited by oocytes in the experimental group 1 without ACP-318® (p < 0.05). The results suggest that oocytes’ nuclear morphology integrity and meiosis achievement were positively influenced when exposed to high glucose TCM199 supplemented with 5% powdered coconut water. Further investigation must be performed for a better understanding of powdered coconut water influence in cellular events during IVM of dog oocytes.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/6868905" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="eb52f04738281a350816d8d223f7d109" rel="nofollow" data-download="{"attachment_id":48691051,"asset_id":6868905,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/48691051/download_file?st=MTczMjc2NjI2Miw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="11494706" href="https://ufu.academia.edu/JLRodrigues">JL Rodrigues</a><script data-card-contents-for-user="11494706" type="text/json">{"id":11494706,"first_name":"JL","last_name":"Rodrigues","domain_name":"ufu","page_name":"JLRodrigues","display_name":"JL Rodrigues","profile_url":"https://ufu.academia.edu/JLRodrigues?f_ri=956315","photo":"https://0.academia-photos.com/11494706/8366253/9355272/s65_jl.rodrigues.jpg_oh_214d662ff1ef0e6f6a0fa5dc78c2c6ba_oe_55a1f2d8___gda___1436065854_c302efcb75278a0d1f4dbe23ed2731a3"}</script></span></span></li><li class="js-paper-rank-work_6868905 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="6868905"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 6868905, container: ".js-paper-rank-work_6868905", }); 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$(".js-view-count[data-work-id=6868905]").text(description); $(".js-view-count-work_6868905").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_6868905").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="6868905"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i> <a class="InlineList-item-text u-positionRelative">8</a> </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="2215" href="https://www.academia.edu/Documents/in/Water">Water</a>, <script data-card-contents-for-ri="2215" type="text/json">{"id":2215,"name":"Water","url":"https://www.academia.edu/Documents/in/Water?f_ri=956315","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="4559" href="https://www.academia.edu/Documents/in/Reproduction">Reproduction</a>, <script data-card-contents-for-ri="4559" type="text/json">{"id":4559,"name":"Reproduction","url":"https://www.academia.edu/Documents/in/Reproduction?f_ri=956315","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="47884" href="https://www.academia.edu/Documents/in/Biological_Sciences">Biological Sciences</a>, <script data-card-contents-for-ri="47884" type="text/json">{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=956315","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="52438" href="https://www.academia.edu/Documents/in/Dogs">Dogs</a><script data-card-contents-for-ri="52438" type="text/json">{"id":52438,"name":"Dogs","url":"https://www.academia.edu/Documents/in/Dogs?f_ri=956315","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=6868905]'), work: {"id":6868905,"title":"The Influence of Powdered Coconut Water (ACP318 ® ) in In Vitro Maturation of Canine Oocytes","created_at":"2014-04-25T04:33:24.052-07:00","url":"https://www.academia.edu/6868905/The_Influence_of_Powdered_Coconut_Water_ACP318_in_In_Vitro_Maturation_of_Canine_Oocytes?f_ri=956315","dom_id":"work_6868905","summary":"The objective of this study was to determine the influence of powdered coconut water (ACP-318®) diluted in high glucose (11.0 mm) TCM199 in the achievement of nuclear in vitro maturation (IVM) of canine oocytes. Cumulus oocyte complexes (COCs) (n = 632) were randomly allocated into three experimental groups named as group 1 (control group), group 2 (5% powdered coconut water) and group 3 (10% powdered coconut water). The percentage of meiotic resumption (MR) (GVBD to MII) was 39.1% (81/207), 50.2% (108/215) and 46.6% (98/210) for groups 1, 2 and 3 respectively (p \u003c 0.05). There were no differences in MR rates among groups 2 and 3. The medium with ACP-318® slightly enhanced the nuclear maturation of canine oocytes when a comparison was established with rates of maturation exhibited by oocytes in the experimental group 1 without ACP-318® (p \u003c 0.05). The results suggest that oocytes’ nuclear morphology integrity and meiosis achievement were positively influenced when exposed to high glucose TCM199 supplemented with 5% powdered coconut water. Further investigation must be performed for a better understanding of powdered coconut water influence in cellular events during IVM of dog oocytes.","downloadable_attachments":[{"id":48691051,"asset_id":6868905,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":11494706,"first_name":"JL","last_name":"Rodrigues","domain_name":"ufu","page_name":"JLRodrigues","display_name":"JL Rodrigues","profile_url":"https://ufu.academia.edu/JLRodrigues?f_ri=956315","photo":"https://0.academia-photos.com/11494706/8366253/9355272/s65_jl.rodrigues.jpg_oh_214d662ff1ef0e6f6a0fa5dc78c2c6ba_oe_55a1f2d8___gda___1436065854_c302efcb75278a0d1f4dbe23ed2731a3"}],"research_interests":[{"id":2215,"name":"Water","url":"https://www.academia.edu/Documents/in/Water?f_ri=956315","nofollow":false},{"id":4559,"name":"Reproduction","url":"https://www.academia.edu/Documents/in/Reproduction?f_ri=956315","nofollow":false},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=956315","nofollow":false},{"id":52438,"name":"Dogs","url":"https://www.academia.edu/Documents/in/Dogs?f_ri=956315","nofollow":false},{"id":73564,"name":"Meiosis","url":"https://www.academia.edu/Documents/in/Meiosis?f_ri=956315"},{"id":143078,"name":"Cocos","url":"https://www.academia.edu/Documents/in/Cocos?f_ri=956315"},{"id":956315,"name":"Oocytes","url":"https://www.academia.edu/Documents/in/Oocytes?f_ri=956315"},{"id":957359,"name":"Culture Media","url":"https://www.academia.edu/Documents/in/Culture_Media?f_ri=956315"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_9093230" data-work_id="9093230" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/9093230/31P_Nuclear_Magnetic_Resonance_Studies_on_the_Developing_Embryos_of_Xenopus_laevis">31P Nuclear-Magnetic-Resonance Studies on the Developing Embryos of Xenopus laevis</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/9093230" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="15fd9ccd7a9398a60d7b23dadebf2cd1" rel="nofollow" data-download="{"attachment_id":47889423,"asset_id":9093230,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button 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itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/23837494/_Genotoxicity_risk_assessment_and_oocytes_basis_of_genetic_toxicology_and_application_in_reproductive_science_">[Genotoxicity risk assessment and oocytes: basis of genetic toxicology and application in reproductive science]</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">The aim of genotoxicity tests in germ cells is to assess the impact of exposure to environmental mutagens that may represent a risk for the fertility or for the offspring of exposed subject. The comet assay on mature mouse oocytes is a... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_23837494" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">The aim of genotoxicity tests in germ cells is to assess the impact of exposure to environmental mutagens that may represent a risk for the fertility or for the offspring of exposed subject. The comet assay on mature mouse oocytes is a simple, reproductive and rapid test to study primary DNA damage in oocytes. This test is used to complete toxicology assays applied in first line to somatic cells, and could find many applications in reproductive toxicology to study impact of environmental factors on female germ cells. We describe a practical application of comet assay in reproductive biology to assess the genotoxicity of cryoprotectants used at high concentrations in oocyte vitrification protocols. This test allowed us to demonstrate that dimethylsulfoxide and ethylene glycol are non-genotoxic for the mouse oocytes and led us to hypothesize a genotoxic effect of 1,2-propanediol (PrOH) at high concentrations after having observed induction of significant DNA damage on CHO cell line an...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/23837494" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="3be4a1624b50e69a45e78fd484efa803" rel="nofollow" data-download="{"attachment_id":44237957,"asset_id":23837494,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/44237957/download_file?st=MTczMjc2NjI2Miw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="46082625" href="https://independent.academia.edu/JeannePerrin1">Jeanne Perrin</a><script data-card-contents-for-user="46082625" type="text/json">{"id":46082625,"first_name":"Jeanne","last_name":"Perrin","domain_name":"independent","page_name":"JeannePerrin1","display_name":"Jeanne Perrin","profile_url":"https://independent.academia.edu/JeannePerrin1?f_ri=956315","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_23837494 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="23837494"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 23837494, container: ".js-paper-rank-work_23837494", }); 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The comet assay on mature mouse oocytes is a simple, reproductive and rapid test to study primary DNA damage in oocytes. This test is used to complete toxicology assays applied in first line to somatic cells, and could find many applications in reproductive toxicology to study impact of environmental factors on female germ cells. We describe a practical application of comet assay in reproductive biology to assess the genotoxicity of cryoprotectants used at high concentrations in oocyte vitrification protocols. 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href="https://www.academia.edu/17434594/New_site_of_temporary_storage_of_rRNA_inO_mykiss_previtellogenic_oocytes">New site of temporary storage of rRNA inO. mykiss previtellogenic oocytes</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/17434594" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="dd0888a87f1316c306d240ac4686e3f8" rel="nofollow" data-download="{"attachment_id":39506266,"asset_id":17434594,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm 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u-tcGrayDarkest"><div class="summarized">Scientists are able to advance the frontiers of human reproduction by employing a variety of molecular biological techniques to understand the biological processes intricately linked to oocyte and ovarian follicle development. Despite an... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_73275675" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Scientists are able to advance the frontiers of human reproduction by employing a variety of molecular biological techniques to understand the biological processes intricately linked to oocyte and ovarian follicle development. Despite an abundance of knowledge concerning essential pathways which may have critical roles in oogenesis and folliculogenesis, the repertoire of medications to treat female fertility problems remains limited to a few classes of drugs involved in the induction or suppression of folliculogenesis and ovulation; many of which are not specific in their drug actions and can give rise to complications during clinical application. This paper aims to review biomolecules and pathways (e.g. PI3K, WNT, MAPK) pertinent to ovarian follicular development and active in human oocytes, including those involved in communication between somatic cells within the ovary (cumulus cells, granulosa cells and thecal cells) and the oocyte itself. These biomolecules which are involved in the nuclear and cytoplasmic maturation of oocytes and the control of ovarian folliculogenesis have potential as targets for improved ovarian stimulation, optimisation of oocyte maturation, and as biomarkers of oocyte viability assessment.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/73275675" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="49290839" href="https://independent.academia.edu/ZhongweiHuang">Zhongwei Huang</a><script data-card-contents-for-user="49290839" type="text/json">{"id":49290839,"first_name":"Zhongwei","last_name":"Huang","domain_name":"independent","page_name":"ZhongweiHuang","display_name":"Zhongwei Huang","profile_url":"https://independent.academia.edu/ZhongweiHuang?f_ri=956315","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_73275675 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="73275675"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 73275675, container: ".js-paper-rank-work_73275675", }); 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Despite an abundance of knowledge concerning essential pathways which may have critical roles in oogenesis and folliculogenesis, the repertoire of medications to treat female fertility problems remains limited to a few classes of drugs involved in the induction or suppression of folliculogenesis and ovulation; many of which are not specific in their drug actions and can give rise to complications during clinical application. This paper aims to review biomolecules and pathways (e.g. PI3K, WNT, MAPK) pertinent to ovarian follicular development and active in human oocytes, including those involved in communication between somatic cells within the ovary (cumulus cells, granulosa cells and thecal cells) and the oocyte itself. These biomolecules which are involved in the nuclear and cytoplasmic maturation of oocytes and the control of ovarian folliculogenesis have potential as targets for improved ovarian stimulation, optimisation of oocyte maturation, and as biomarkers of oocyte viability assessment.","downloadable_attachments":[],"ordered_authors":[{"id":49290839,"first_name":"Zhongwei","last_name":"Huang","domain_name":"independent","page_name":"ZhongweiHuang","display_name":"Zhongwei Huang","profile_url":"https://independent.academia.edu/ZhongweiHuang?f_ri=956315","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":8954,"name":"Fertility","url":"https://www.academia.edu/Documents/in/Fertility?f_ri=956315","nofollow":false},{"id":98943,"name":"Ovulation Induction","url":"https://www.academia.edu/Documents/in/Ovulation_Induction?f_ri=956315","nofollow":false},{"id":120789,"name":"Drug 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class="summarized">To analyze whether a change in three-dimensional structure of the zona pellucida could indicate suboptimal gamete quality. Prospective study. Women&amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;s general hospital. A total of 72 patients who... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_19299141" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">To analyze whether a change in three-dimensional structure of the zona pellucida could indicate suboptimal gamete quality. Prospective study. Women&amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;s general hospital. A total of 72 patients who gave informed consent. The birefringence of all oocytes was prospectively analyzed with an automatic user-independent polarization microscopy imaging system. Birefringence of the inner zona layer, preimplantation development, implantation, and pregnancy. In approximately one third of all gametes (244/712), the system&amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;s automatic detection of the inner zona layer did not succeed. This phenomenon was a negative predictor of compaction (P&amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;0.01), blastulation (P&amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;0.001), and pregnancy (P&amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;0.001). In cases of successful zona imaging, the score based on the birefringence of the inner zona layer was a strong predictor of blastocyst formation but not of embryo quality or pregnancy (P&amp;amp;amp;amp;amp;amp;amp;amp;amp;gt;0.05). Interestingly, antagonist protocol resulted in lower zona scores as compared with the long protocol (P&amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;0.05). Combining the information from both undetected and detected oocytes, zona imaging was a helpful tool in oocyte selection. This knowledge might further help to reduce both the time in culture and the number of concepti considered for transfer.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/19299141" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="653f809ed044c1cbf71c356ae319d623" rel="nofollow" data-download="{"attachment_id":42099631,"asset_id":19299141,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/42099631/download_file?st=MTczMjc2NjI2Miw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="39554479" href="https://ku.academia.edu/BulentUrman">Bulent Urman</a><script data-card-contents-for-user="39554479" type="text/json">{"id":39554479,"first_name":"Bulent","last_name":"Urman","domain_name":"ku","page_name":"BulentUrman","display_name":"Bulent Urman","profile_url":"https://ku.academia.edu/BulentUrman?f_ri=956315","photo":"https://0.academia-photos.com/39554479/10883563/12145835/s65_bulent.urman.jpg"}</script></span></span></li><li class="js-paper-rank-work_19299141 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="19299141"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 19299141, container: ".js-paper-rank-work_19299141", }); 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