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class="profile--tab_heading_container">Papers by Thais Souto-padrón</h3></div><div class="js-work-strip profile--work_container" data-work-id="13178851"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/13178851/Further_Studies_on_the_Organization_of_the_Paraxial_Rod_of_Trypanosomatids_1"><img alt="Research paper thumbnail of Further Studies on the Organization of the Paraxial Rod of Trypanosomatids 1" class="work-thumbnail" src="https://attachments.academia-assets.com/45684237/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/13178851/Further_Studies_on_the_Organization_of_the_Paraxial_Rod_of_Trypanosomatids_1">Further Studies on the Organization of the Paraxial Rod of Trypanosomatids 1</a></div><div class="wp-workCard_item"><span>The Journal of Protozoology</span><span>, 1986</span></div><div class="wp-workCard_item"><div class="carousel-container carousel-container--sm" id="profile-work-13178851-figures"><div class="prev-slide-container js-prev-button-container"><button aria-label="Previous" class="carousel-navigation-button js-profile-work-13178851-figures-prev"><span class="material-symbols-outlined" style="font-size: 24px" translate="no">arrow_back_ios</span></button></div><div class="slides-container js-slides-container"><figure class="figure-slide-container"><a href="https://www.academia.edu/figures/4387302/figure-1-further-studies-on-the-organization-of-the-paraxial"><img alt="" class="figure-slide-image" src="https://figures.academia-assets.com/45684237/figure_001.jpg" /></a></figure><figure class="figure-slide-container"><a href="https://www.academia.edu/figures/4387305/figure-2-farina-et-al-paraxial-rod-structure-of"><img alt="FARINA ET AL.—PARAXIAL ROD STRUCTURE OF TRYPANOSOMATIDS " class="figure-slide-image" src="https://figures.academia-assets.com/45684237/figure_002.jpg" /></a></figure></div><div class="next-slide-container js-next-button-container"><button aria-label="Next" class="carousel-navigation-button js-profile-work-13178851-figures-next"><span class="material-symbols-outlined" style="font-size: 24px" translate="no">arrow_forward_ios</span></button></div></div></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="2f727b048b982938652ef0823684de9a" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":45684237,"asset_id":13178851,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/45684237/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") if (true) { Aedu.setUpFigureCarousel('profile-work-13178851-figures'); } }); </script> <div class="js-work-strip profile--work_container" data-work-id="13178850"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/13178850/Structure_and_expression_of_two_Trypanosoma_cruzi_genes_encoding_antigenic_proteins_bearing_repetitive_epitopes"><img alt="Research paper thumbnail of Structure and expression of two Trypanosoma cruzi genes encoding antigenic proteins bearing repetitive epitopes" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title">Structure and expression of two Trypanosoma cruzi genes encoding antigenic proteins bearing repetitive epitopes</div><div class="wp-workCard_item"><span>Molecular and Biochemical Parasitology</span><span>, 1989</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Trypanosoma cruzi genes were cloned in lambda gt11 and screened with an anti-trypomastigote antis...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Trypanosoma cruzi genes were cloned in lambda gt11 and screened with an anti-trypomastigote antiserum. Two out of twelve clones were selected in view of their reactivity with human chagasic sera. One clone encodes a flagellar antigen (FRA) of more than 300 kDa, whereas the other corresponds to a roughly 225-kDa cytoplasmic antigen (CRA). The flagellar antigen is present in both epimastigotes and trypomastigotes, but the cytoplasmic antigen is not found in trypomastigotes. The CRA clone is entirely composed of at least 23 copies of a 42-bp repeat and the FRA gene contains at least 14 copies of a 204-bp motif. The FRA gene hybridizes to a RNA of about 10 kb, while the CRA gene detects a transcript of 5.2 kb.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="13178850"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="13178850"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 13178850; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=13178850]").text(description); $(".js-view-count[data-work-id=13178850]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 13178850; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='13178850']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=13178850]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":13178850,"title":"Structure and expression of two Trypanosoma cruzi genes encoding antigenic proteins bearing repetitive epitopes","translated_title":"","metadata":{"abstract":"Trypanosoma cruzi genes were cloned in lambda gt11 and screened with an anti-trypomastigote antiserum. Two out of twelve clones were selected in view of their reactivity with human chagasic sera. One clone encodes a flagellar antigen (FRA) of more than 300 kDa, whereas the other corresponds to a roughly 225-kDa cytoplasmic antigen (CRA). The flagellar antigen is present in both epimastigotes and trypomastigotes, but the cytoplasmic antigen is not found in trypomastigotes. The CRA clone is entirely composed of at least 23 copies of a 42-bp repeat and the FRA gene contains at least 14 copies of a 204-bp motif. The FRA gene hybridizes to a RNA of about 10 kb, while the CRA gene detects a transcript of 5.2 kb.","publication_date":{"day":null,"month":null,"year":1989,"errors":{}},"publication_name":"Molecular and Biochemical Parasitology"},"translated_abstract":"Trypanosoma cruzi genes were cloned in lambda gt11 and screened with an anti-trypomastigote antiserum. Two out of twelve clones were selected in view of their reactivity with human chagasic sera. One clone encodes a flagellar antigen (FRA) of more than 300 kDa, whereas the other corresponds to a roughly 225-kDa cytoplasmic antigen (CRA). The flagellar antigen is present in both epimastigotes and trypomastigotes, but the cytoplasmic antigen is not found in trypomastigotes. The CRA clone is entirely composed of at least 23 copies of a 42-bp repeat and the FRA gene contains at least 14 copies of a 204-bp motif. The FRA gene hybridizes to a RNA of about 10 kb, while the CRA gene detects a transcript of 5.2 kb.","internal_url":"https://www.academia.edu/13178850/Structure_and_expression_of_two_Trypanosoma_cruzi_genes_encoding_antigenic_proteins_bearing_repetitive_epitopes","translated_internal_url":"","created_at":"2015-06-22T10:19:21.356-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":32431858,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":1360947,"work_id":13178850,"tagging_user_id":32431858,"tagged_user_id":null,"co_author_invite_id":258348,"email":"w***a@sect.rj.gov.br","display_order":null,"name":"Wanderley Souza","title":"Structure and expression of two Trypanosoma cruzi genes encoding antigenic proteins bearing repetitive epitopes"},{"id":1360924,"work_id":13178850,"tagging_user_id":32431858,"tagged_user_id":31729059,"co_author_invite_id":null,"email":"w***a@biof.ufrj.br","display_order":null,"name":"Wanderley de Souza","title":"Structure and expression of two Trypanosoma cruzi genes encoding antigenic proteins bearing repetitive epitopes"},{"id":1361123,"work_id":13178850,"tagging_user_id":32431858,"tagged_user_id":32778051,"co_author_invite_id":133352,"email":"m***r@fiocruz.br","affiliation":"Fundação Oswaldo Cruz","display_order":null,"name":"Marco Krieger","title":"Structure and expression of two Trypanosoma cruzi genes encoding antigenic proteins bearing repetitive epitopes"},{"id":1361013,"work_id":13178850,"tagging_user_id":32431858,"tagged_user_id":32333041,"co_author_invite_id":null,"email":"w***a@gmail.com","display_order":null,"name":"Wanderley Desouza","title":"Structure and expression of two Trypanosoma cruzi genes encoding antigenic proteins bearing repetitive epitopes"},{"id":1361119,"work_id":13178850,"tagging_user_id":32431858,"tagged_user_id":28209640,"co_author_invite_id":133351,"email":"s***1@gmail.com","affiliation":"Instituto Carlos Chagas","display_order":null,"name":"Samuel Goldenberg","title":"Structure and expression of two Trypanosoma cruzi genes encoding antigenic proteins bearing repetitive epitopes"}],"downloadable_attachments":[],"slug":"Structure_and_expression_of_two_Trypanosoma_cruzi_genes_encoding_antigenic_proteins_bearing_repetitive_epitopes","translated_slug":"","page_count":null,"language":"en","content_type":"Work","summary":"Trypanosoma cruzi genes were cloned in lambda gt11 and screened with an anti-trypomastigote antiserum. Two out of twelve clones were selected in view of their reactivity with human chagasic sera. One clone encodes a flagellar antigen (FRA) of more than 300 kDa, whereas the other corresponds to a roughly 225-kDa cytoplasmic antigen (CRA). The flagellar antigen is present in both epimastigotes and trypomastigotes, but the cytoplasmic antigen is not found in trypomastigotes. The CRA clone is entirely composed of at least 23 copies of a 42-bp repeat and the FRA gene contains at least 14 copies of a 204-bp motif. 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Catalase activity, which is inhibited by 3-amino-1, 2, 4-triazole, was detected at the ultrastructural level in the matrix of the organelle by using an alkaline diaminobenzidine medium. Freeze-fracture studies showed the presence of a large number of intramembranous particles on both the P and the E faces of the membrane of the organelle. Based on these data as well as on previous observations, it is suggested that the trypanosomatids possess an organelle that can be considered to be a peroxisome.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="13178849"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="13178849"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 13178849; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=13178849]").text(description); $(".js-view-count[data-work-id=13178849]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 13178849; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='13178849']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=13178849]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":13178849,"title":"Fine structure and cytochemistry of peroxisomes (microbodies) in Leptomonas samueli","translated_title":"","metadata":{"abstract":"Leptomonas samueli possesses in its cytoplasm a membrane-bounded organelle which can reach a length of 2.8 microns and a diameter of 0.2 microns. Catalase activity, which is inhibited by 3-amino-1, 2, 4-triazole, was detected at the ultrastructural level in the matrix of the organelle by using an alkaline diaminobenzidine medium. Freeze-fracture studies showed the presence of a large number of intramembranous particles on both the P and the E faces of the membrane of the organelle. Based on these data as well as on previous observations, it is suggested that the trypanosomatids possess an organelle that can be considered to be a peroxisome.","publication_date":{"day":null,"month":null,"year":1982,"errors":{}}},"translated_abstract":"Leptomonas samueli possesses in its cytoplasm a membrane-bounded organelle which can reach a length of 2.8 microns and a diameter of 0.2 microns. Catalase activity, which is inhibited by 3-amino-1, 2, 4-triazole, was detected at the ultrastructural level in the matrix of the organelle by using an alkaline diaminobenzidine medium. Freeze-fracture studies showed the presence of a large number of intramembranous particles on both the P and the E faces of the membrane of the organelle. Based on these data as well as on previous observations, it is suggested that the trypanosomatids possess an organelle that can be considered to be a peroxisome.","internal_url":"https://www.academia.edu/13178849/Fine_structure_and_cytochemistry_of_peroxisomes_microbodies_in_Leptomonas_samueli","translated_internal_url":"","created_at":"2015-06-22T10:19:20.815-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":32431858,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":1361202,"work_id":13178849,"tagging_user_id":32431858,"tagged_user_id":31729059,"co_author_invite_id":null,"email":"w***a@biof.ufrj.br","display_order":null,"name":"Wanderley de Souza","title":"Fine structure and cytochemistry of peroxisomes (microbodies) in Leptomonas samueli"}],"downloadable_attachments":[],"slug":"Fine_structure_and_cytochemistry_of_peroxisomes_microbodies_in_Leptomonas_samueli","translated_slug":"","page_count":null,"language":"en","content_type":"Work","summary":"Leptomonas samueli possesses in its cytoplasm a membrane-bounded organelle which can reach a length of 2.8 microns and a diameter of 0.2 microns. Catalase activity, which is inhibited by 3-amino-1, 2, 4-triazole, was detected at the ultrastructural level in the matrix of the organelle by using an alkaline diaminobenzidine medium. Freeze-fracture studies showed the presence of a large number of intramembranous particles on both the P and the E faces of the membrane of the organelle. Based on these data as well as on previous observations, it is suggested that the trypanosomatids possess an organelle that can be considered to be a peroxisome.","owner":{"id":32431858,"first_name":"Thais","middle_initials":null,"last_name":"Souto-padrón","page_name":"ThaisSoutopadrón","domain_name":"ufrj","created_at":"2015-06-22T08:20:34.150-07:00","display_name":"Thais Souto-padrón","url":"https://ufrj.academia.edu/ThaisSoutopadr%C3%B3n"},"attachments":[],"research_interests":[{"id":139007,"name":"Catalase","url":"https://www.academia.edu/Documents/in/Catalase"},{"id":186234,"name":"Medical Physiology","url":"https://www.academia.edu/Documents/in/Medical_Physiology"},{"id":317484,"name":"Fine Structure Constant","url":"https://www.academia.edu/Documents/in/Fine_Structure_Constant"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") if (false) { Aedu.setUpFigureCarousel('profile-work-13178849-figures'); } }); </script> <div class="js-work-strip profile--work_container" data-work-id="13178848"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/13178848/Cellular_signaling_during_the_macrophage_invasion_by_Trypanosoma_cruzi"><img alt="Research paper thumbnail of Cellular signaling during the macrophage invasion by Trypanosoma cruzi" class="work-thumbnail" src="https://attachments.academia-assets.com/45684095/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/13178848/Cellular_signaling_during_the_macrophage_invasion_by_Trypanosoma_cruzi">Cellular signaling during the macrophage invasion by Trypanosoma cruzi</a></div><div class="wp-workCard_item"><span>Histochemistry and cell biology</span><span>, 2002</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">We have reported that protein tyrosine kinases play an important role in the invasion of Trypanos...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">We have reported that protein tyrosine kinases play an important role in the invasion of Trypanosoma cruzi into primary resident macrophages. In the present study we carry out immunofluorescence assays, using monoclonal anti-phosphotyrosine antibodies, to reveal an accumulation of tyrosine-phosphorylated residues at the site of parasite association with the macrophage surface, colocalizing with host cell F-actin-rich domains. SDS-PAGE analysis of macrophage cell line IC-21 tyrosine phosphoproteins, labeled with [(35)S] L-methionine, revealed several peptides with increased levels of phosphorylation upon interaction with the parasite. Among them, were detected bands of 140, 120, 112, 94, 73, 67, and 56 kDa that match the molecular weights of proteins described as being tyrosine phosphorylated during events that lead to actin assembly in mononuclear phagocytes. The pretreatment of IC-21 macrophages with the tyrosine kinase inhibitor tyrphostin 23 inhibited trypomastigote uptake showin...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="2eac546fea31061980c5d97a6a3f505d" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":45684095,"asset_id":13178848,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/45684095/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="13178848"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="13178848"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 13178848; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=13178848]").text(description); $(".js-view-count[data-work-id=13178848]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 13178848; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='13178848']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "2eac546fea31061980c5d97a6a3f505d" } } $('.js-work-strip[data-work-id=13178848]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":13178848,"title":"Cellular signaling during the macrophage invasion by Trypanosoma cruzi","translated_title":"","metadata":{"abstract":"We have reported that protein tyrosine kinases play an important role in the invasion of Trypanosoma cruzi into primary resident macrophages. In the present study we carry out immunofluorescence assays, using monoclonal anti-phosphotyrosine antibodies, to reveal an accumulation of tyrosine-phosphorylated residues at the site of parasite association with the macrophage surface, colocalizing with host cell F-actin-rich domains. SDS-PAGE analysis of macrophage cell line IC-21 tyrosine phosphoproteins, labeled with [(35)S] L-methionine, revealed several peptides with increased levels of phosphorylation upon interaction with the parasite. Among them, were detected bands of 140, 120, 112, 94, 73, 67, and 56 kDa that match the molecular weights of proteins described as being tyrosine phosphorylated during events that lead to actin assembly in mononuclear phagocytes. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") if (false) { Aedu.setUpFigureCarousel('profile-work-13178848-figures'); } }); </script> <div class="js-work-strip profile--work_container" data-work-id="13178847"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/13178847/Structural_and_functional_analysis_of_a_platelet_activating_lysophosphatidylcholine_of_Trypanosoma_cruzi"><img alt="Research paper thumbnail of Structural and functional analysis of a platelet-activating lysophosphatidylcholine of Trypanosoma cruzi" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title">Structural and functional analysis of a platelet-activating lysophosphatidylcholine of Trypanosoma cruzi</div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/MauricioOliveira25">Mauricio Oliveira</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://ufmg.academia.edu/AlexandreMarques">Alexandre Marques</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://ufrj.academia.edu/ThaisSoutopadr%C3%B3n">Thais Souto-padrón</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://utep.academia.edu/IAlmeida">Igor C Almeida</a>, and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/MartaGomes18">Marta Gomes</a></span></div><div class="wp-workCard_item"><span>PLoS neglected tropical diseases</span><span>, 2014</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Trypanosoma cruzi is the causative agent of the life-threatening Chagas disease, in which increas...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Trypanosoma cruzi is the causative agent of the life-threatening Chagas disease, in which increased platelet aggregation related to myocarditis is observed. Platelet-activating factor (PAF) is a potent intercellular lipid mediator and second messenger that exerts its activity through a PAF-specific receptor (PAFR). Previous data from our group suggested that T. cruzi synthesizes a phospholipid with PAF-like activity. The structure of T. cruzi PAF-like molecule, however, remains elusive. Here, we have purified and structurally characterized the putative T. cruzi PAF-like molecule by electrospray ionization-tandem mass spectrometry (ESI-MS/MS). Our ESI-MS/MS data demonstrated that the T. cruzi PAF-like molecule is actually a lysophosphatidylcholine (LPC), namely sn-1 C18:1(delta 9)-LPC. Similar to PAF, the platelet-aggregating activity of C18:1-LPC was abrogated by the PAFR antagonist, WEB 2086. Other major LPC species, i.e., C16:0-, C18:0-, and C18:2-LPC, were also characterized in a...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="13178847"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="13178847"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 13178847; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=13178847]").text(description); $(".js-view-count[data-work-id=13178847]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 13178847; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='13178847']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=13178847]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":13178847,"title":"Structural and functional analysis of a platelet-activating lysophosphatidylcholine of Trypanosoma cruzi","translated_title":"","metadata":{"abstract":"Trypanosoma cruzi is the causative agent of the life-threatening Chagas disease, in which increased platelet aggregation related to myocarditis is observed. 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Platelet-activating factor (PAF) is a potent intercellular lipid mediator and second messenger that exerts its activity through a PAF-specific receptor (PAFR). Previous data from our group suggested that T. cruzi synthesizes a phospholipid with PAF-like activity. The structure of T. cruzi PAF-like molecule, however, remains elusive. Here, we have purified and structurally characterized the putative T. cruzi PAF-like molecule by electrospray ionization-tandem mass spectrometry (ESI-MS/MS). Our ESI-MS/MS data demonstrated that the T. cruzi PAF-like molecule is actually a lysophosphatidylcholine (LPC), namely sn-1 C18:1(delta 9)-LPC. Similar to PAF, the platelet-aggregating activity of C18:1-LPC was abrogated by the PAFR antagonist, WEB 2086. Other major LPC species, i.e., C16:0-, C18:0-, and C18:2-LPC, were also characterized in a...","owner":{"id":32431858,"first_name":"Thais","middle_initials":null,"last_name":"Souto-padrón","page_name":"ThaisSoutopadrón","domain_name":"ufrj","created_at":"2015-06-22T08:20:34.150-07:00","display_name":"Thais Souto-padrón","url":"https://ufrj.academia.edu/ThaisSoutopadr%C3%B3n"},"attachments":[],"research_interests":[{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences"},{"id":65641,"name":"Platelet aggregation","url":"https://www.academia.edu/Documents/in/Platelet_aggregation"},{"id":86313,"name":"G protein-coupled receptors","url":"https://www.academia.edu/Documents/in/G_protein-coupled_receptors"},{"id":163275,"name":"Tandem Mass Spectrometry","url":"https://www.academia.edu/Documents/in/Tandem_Mass_Spectrometry"},{"id":326380,"name":"Liquid Chromatography / Electrospray Ionization Mass Spectrometry","url":"https://www.academia.edu/Documents/in/Liquid_Chromatography_Electrospray_Ionization_Mass_Spectrometry"},{"id":376084,"name":"Trypanosoma Cruzi","url":"https://www.academia.edu/Documents/in/Trypanosoma_Cruzi"},{"id":788677,"name":"Rabbits","url":"https://www.academia.edu/Documents/in/Rabbits"},{"id":801411,"name":"Triazoles","url":"https://www.academia.edu/Documents/in/Triazoles"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") if (false) { Aedu.setUpFigureCarousel('profile-work-13178847-figures'); } }); </script> <div class="js-work-strip profile--work_container" data-work-id="13178845"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/13178845/The_effect_of_tunicamycin_and_monensin_on_the_association_of_Trypanosoma_cruzi_with_resident_macrophages"><img alt="Research paper thumbnail of The effect of tunicamycin and monensin on the association of Trypanosoma cruzi with resident macrophages" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title">The effect of tunicamycin and monensin on the association of Trypanosoma cruzi with resident macrophages</div><div class="wp-workCard_item"><span>Parasitology Research</span><span>, 1989</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The effect of incubation of parasites (epimastigote and trypomastigote forms ofTrypanosoma cruzi)...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The effect of incubation of parasites (epimastigote and trypomastigote forms ofTrypanosoma cruzi) or macrophages in the presence of tunicamycin (TM) or monensin (M) on the parasite-macrophage association was analysed. Treatment of the parasites with TM, a drug which interferes with the process ofN-glycosylation of proteins, increased by about 70% and decreased by about 27% the infection of epimastigote and trypomastigote</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="13178845"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="13178845"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 13178845; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=13178845]").text(description); $(".js-view-count[data-work-id=13178845]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 13178845; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='13178845']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=13178845]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":13178845,"title":"The effect of tunicamycin and monensin on the association of Trypanosoma cruzi with resident macrophages","translated_title":"","metadata":{"abstract":"The effect of incubation of parasites (epimastigote and trypomastigote forms ofTrypanosoma cruzi) or macrophages in the presence of tunicamycin (TM) or monensin (M) on the parasite-macrophage association was analysed. Treatment of the parasites with TM, a drug which interferes with the process ofN-glycosylation of proteins, increased by about 70% and decreased by about 27% the infection of epimastigote and trypomastigote","publication_date":{"day":null,"month":null,"year":1989,"errors":{}},"publication_name":"Parasitology Research"},"translated_abstract":"The effect of incubation of parasites (epimastigote and trypomastigote forms ofTrypanosoma cruzi) or macrophages in the presence of tunicamycin (TM) or monensin (M) on the parasite-macrophage association was analysed. Treatment of the parasites with TM, a drug which interferes with the process ofN-glycosylation of proteins, increased by about 70% and decreased by about 27% the infection of epimastigote and trypomastigote","internal_url":"https://www.academia.edu/13178845/The_effect_of_tunicamycin_and_monensin_on_the_association_of_Trypanosoma_cruzi_with_resident_macrophages","translated_internal_url":"","created_at":"2015-06-22T10:19:19.319-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":32431858,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":1361024,"work_id":13178845,"tagging_user_id":32431858,"tagged_user_id":32333041,"co_author_invite_id":null,"email":"w***a@gmail.com","display_order":null,"name":"Wanderley Desouza","title":"The effect of tunicamycin and monensin on the association of Trypanosoma cruzi with resident macrophages"}],"downloadable_attachments":[],"slug":"The_effect_of_tunicamycin_and_monensin_on_the_association_of_Trypanosoma_cruzi_with_resident_macrophages","translated_slug":"","page_count":null,"language":"en","content_type":"Work","summary":"The effect of incubation of parasites (epimastigote and trypomastigote forms ofTrypanosoma cruzi) or macrophages in the presence of tunicamycin (TM) or monensin (M) on the parasite-macrophage association was analysed. Treatment of the parasites with TM, a drug which interferes with the process ofN-glycosylation of proteins, increased by about 70% and decreased by about 27% the infection of epimastigote and trypomastigote","owner":{"id":32431858,"first_name":"Thais","middle_initials":null,"last_name":"Souto-padrón","page_name":"ThaisSoutopadrón","domain_name":"ufrj","created_at":"2015-06-22T08:20:34.150-07:00","display_name":"Thais Souto-padrón","url":"https://ufrj.academia.edu/ThaisSoutopadr%C3%B3n"},"attachments":[],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology"},{"id":164,"name":"Parasitology","url":"https://www.academia.edu/Documents/in/Parasitology"},{"id":2184,"name":"Electron Microscopy","url":"https://www.academia.edu/Documents/in/Electron_Microscopy"},{"id":6947,"name":"Medical Microbiology","url":"https://www.academia.edu/Documents/in/Medical_Microbiology"},{"id":11558,"name":"Drug interactions","url":"https://www.academia.edu/Documents/in/Drug_interactions"},{"id":17491,"name":"Macrophages","url":"https://www.academia.edu/Documents/in/Macrophages"},{"id":90156,"name":"Endocytosis","url":"https://www.academia.edu/Documents/in/Endocytosis"},{"id":134418,"name":"Tunicamycin","url":"https://www.academia.edu/Documents/in/Tunicamycin"},{"id":167158,"name":"Trypsin","url":"https://www.academia.edu/Documents/in/Trypsin"},{"id":376084,"name":"Trypanosoma Cruzi","url":"https://www.academia.edu/Documents/in/Trypanosoma_Cruzi"},{"id":644860,"name":"Veterinary Sciences","url":"https://www.academia.edu/Documents/in/Veterinary_Sciences"},{"id":900881,"name":"Membrane Protein","url":"https://www.academia.edu/Documents/in/Membrane_Protein"},{"id":979301,"name":"Golgi Apparatus","url":"https://www.academia.edu/Documents/in/Golgi_Apparatus"},{"id":2428330,"name":"Vacuoles","url":"https://www.academia.edu/Documents/in/Vacuoles"}],"urls":[{"id":4894876,"url":"http://www.springerlink.com/index/u78k728743412t30.pdf"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") if (false) { Aedu.setUpFigureCarousel('profile-work-13178845-figures'); } }); </script> <div class="js-work-strip profile--work_container" data-work-id="13178844"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/13178844/Localization_of_lectin_binding_sites_on_the_surface_of_Trypanosoma_cruzi_grown_in_chemically_defined_conditions"><img alt="Research paper thumbnail of Localization of lectin-binding sites on the surface of Trypanosoma cruzi grown in chemically defined conditions" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title">Localization of lectin-binding sites on the surface of Trypanosoma cruzi grown in chemically defined conditions</div><div class="wp-workCard_item"><span>Histochemistry and Cell Biology</span><span>, 1998</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The transformation of Trypanosoma cruzi epimastigotes to mammal-infective metacyclic trypomastigo...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The transformation of Trypanosoma cruzi epimastigotes to mammal-infective metacyclic trypomastigotes (metacyclogenesis) can be performed in vitro under chemically defined conditions (TAU 3AAG medium). During this process, changes in the nature of cell surface sugar composition and sugar distribution was evaluated using FITC and gold-labeled lectins and observed by flow cytometry and transmission electron microscopy. The pattern of labeling with the</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="13178844"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="13178844"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 13178844; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=13178844]").text(description); $(".js-view-count[data-work-id=13178844]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 13178844; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='13178844']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=13178844]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":13178844,"title":"Localization of lectin-binding sites on the surface of Trypanosoma cruzi grown in chemically defined conditions","translated_title":"","metadata":{"abstract":"The transformation of Trypanosoma cruzi epimastigotes to mammal-infective metacyclic trypomastigotes (metacyclogenesis) can be performed in vitro under chemically defined conditions (TAU 3AAG medium). During this process, changes in the nature of cell surface sugar composition and sugar distribution was evaluated using FITC and gold-labeled lectins and observed by flow cytometry and transmission electron microscopy. The pattern of labeling with the","publication_date":{"day":null,"month":null,"year":1998,"errors":{}},"publication_name":"Histochemistry and Cell Biology"},"translated_abstract":"The transformation of Trypanosoma cruzi epimastigotes to mammal-infective metacyclic trypomastigotes (metacyclogenesis) can be performed in vitro under chemically defined conditions (TAU 3AAG medium). During this process, changes in the nature of cell surface sugar composition and sugar distribution was evaluated using FITC and gold-labeled lectins and observed by flow cytometry and transmission electron microscopy. The pattern of labeling with the","internal_url":"https://www.academia.edu/13178844/Localization_of_lectin_binding_sites_on_the_surface_of_Trypanosoma_cruzi_grown_in_chemically_defined_conditions","translated_internal_url":"","created_at":"2015-06-22T10:19:18.841-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":32431858,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":1360939,"work_id":13178844,"tagging_user_id":32431858,"tagged_user_id":31729059,"co_author_invite_id":null,"email":"w***a@biof.ufrj.br","display_order":null,"name":"Wanderley de Souza","title":"Localization of lectin-binding sites on the surface of Trypanosoma cruzi grown in chemically defined conditions"},{"id":1361029,"work_id":13178844,"tagging_user_id":32431858,"tagged_user_id":32333041,"co_author_invite_id":null,"email":"w***a@gmail.com","display_order":null,"name":"Wanderley Desouza","title":"Localization of lectin-binding sites on the surface of Trypanosoma cruzi grown in chemically defined conditions"},{"id":1360962,"work_id":13178844,"tagging_user_id":32431858,"tagged_user_id":null,"co_author_invite_id":258348,"email":"w***a@sect.rj.gov.br","display_order":null,"name":"Wanderley Souza","title":"Localization of lectin-binding sites on the surface of Trypanosoma cruzi grown in chemically defined conditions"}],"downloadable_attachments":[],"slug":"Localization_of_lectin_binding_sites_on_the_surface_of_Trypanosoma_cruzi_grown_in_chemically_defined_conditions","translated_slug":"","page_count":null,"language":"en","content_type":"Work","summary":"The transformation of Trypanosoma cruzi epimastigotes to mammal-infective metacyclic trypomastigotes (metacyclogenesis) can be performed in vitro under chemically defined conditions (TAU 3AAG medium). During this process, changes in the nature of cell surface sugar composition and sugar distribution was evaluated using FITC and gold-labeled lectins and observed by flow cytometry and transmission electron microscopy. The pattern of labeling with the","owner":{"id":32431858,"first_name":"Thais","middle_initials":null,"last_name":"Souto-padrón","page_name":"ThaisSoutopadrón","domain_name":"ufrj","created_at":"2015-06-22T08:20:34.150-07:00","display_name":"Thais Souto-padrón","url":"https://ufrj.academia.edu/ThaisSoutopadr%C3%B3n"},"attachments":[],"research_interests":[{"id":6599,"name":"Flow Cytometry","url":"https://www.academia.edu/Documents/in/Flow_Cytometry"},{"id":14076,"name":"Transmission Electron Microscopy","url":"https://www.academia.edu/Documents/in/Transmission_Electron_Microscopy"},{"id":138998,"name":"Lectins","url":"https://www.academia.edu/Documents/in/Lectins"},{"id":376084,"name":"Trypanosoma Cruzi","url":"https://www.academia.edu/Documents/in/Trypanosoma_Cruzi"},{"id":957359,"name":"Culture Media","url":"https://www.academia.edu/Documents/in/Culture_Media"},{"id":1078292,"name":"Cell Surface Markers","url":"https://www.academia.edu/Documents/in/Cell_Surface_Markers"},{"id":1146416,"name":"Arachis Hypogaea","url":"https://www.academia.edu/Documents/in/Arachis_Hypogaea"},{"id":1242506,"name":"Binding Site","url":"https://www.academia.edu/Documents/in/Binding_Site"}],"urls":[{"id":4894875,"url":"http://www.springerlink.com/index/lq1pe7dqr5kctm3q.pdf"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") if (false) { Aedu.setUpFigureCarousel('profile-work-13178844-figures'); } }); </script> <div class="js-work-strip profile--work_container" data-work-id="13178843"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/13178843/Acetylated_alpha_tubulin_in_Trypanosoma_cruzi_immunocytochemical_localization"><img alt="Research paper thumbnail of Acetylated alpha-tubulin in Trypanosoma cruzi: immunocytochemical localization" class="work-thumbnail" src="https://attachments.academia-assets.com/45612680/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/13178843/Acetylated_alpha_tubulin_in_Trypanosoma_cruzi_immunocytochemical_localization">Acetylated alpha-tubulin in Trypanosoma cruzi: immunocytochemical localization</a></div><div class="wp-workCard_item"><span>Memorias Do Instituto Oswaldo Cruz</span><span>, 1993</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="c6778f016f27745bbd1b4529d698d062" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" 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href="https://www.academia.edu/13178842/Crovirin_a_Snake_Venom_Cysteine_Rich_Secretory_Protein_CRISP_with_Promising_Activity_against_Trypanosomes_and_Leishmania"><img alt="Research paper thumbnail of Crovirin, a Snake Venom Cysteine-Rich Secretory Protein (CRISP) with Promising Activity against Trypanosomes and Leishmania" class="work-thumbnail" src="https://attachments.academia-assets.com/45612678/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/13178842/Crovirin_a_Snake_Venom_Cysteine_Rich_Secretory_Protein_CRISP_with_Promising_Activity_against_Trypanosomes_and_Leishmania">Crovirin, a Snake Venom Cysteine-Rich Secretory Protein (CRISP) with Promising Activity against Trypanosomes and Leishmania</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/JRodrigues4">J. Rodrigues</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/CamilaAdade">Camila Adade</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://ufrj.academia.edu/ThaisSoutopadr%C3%B3n">Thais Souto-padrón</a>, and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/AnaLima74">Ana Lima</a></span></div><div class="wp-workCard_item"><span>PLoS Neglected Tropical Diseases</span><span>, 2014</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Background: The neglected human diseases caused by trypanosomatids are currently treated with tox...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Background: The neglected human diseases caused by trypanosomatids are currently treated with toxic therapy with limited efficacy. In search for novel anti-trypanosomatid agents, we showed previously that the Crotalus viridis viridis (Cvv) snake venom was active against infective forms of Trypanosoma cruzi. Here, we describe the purification of crovirin, a cysteine-rich secretory protein (CRISP) from Cvv venom with promising activity against trypanosomes and Leishmania.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="5e405c9da34f38f02bd5a9282e49e1f3" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":45612678,"asset_id":13178842,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/45612678/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="13178842"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="13178842"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 13178842; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=13178842]").text(description); $(".js-view-count[data-work-id=13178842]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 13178842; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='13178842']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "5e405c9da34f38f02bd5a9282e49e1f3" } } $('.js-work-strip[data-work-id=13178842]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":13178842,"title":"Crovirin, a Snake Venom Cysteine-Rich Secretory Protein (CRISP) with Promising Activity against Trypanosomes and Leishmania","translated_title":"","metadata":{"grobid_abstract":"Background: The neglected human diseases caused by trypanosomatids are currently treated with toxic therapy with limited efficacy. 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Here, we describe the purification of crovirin, a cysteine-rich secretory protein (CRISP) from Cvv venom with promising activity against trypanosomes and Leishmania.","owner":{"id":32431858,"first_name":"Thais","middle_initials":null,"last_name":"Souto-padrón","page_name":"ThaisSoutopadrón","domain_name":"ufrj","created_at":"2015-06-22T08:20:34.150-07:00","display_name":"Thais Souto-padrón","url":"https://ufrj.academia.edu/ThaisSoutopadr%C3%B3n"},"attachments":[{"id":45612678,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/45612678/thumbnails/1.jpg","file_name":"Crovirin_a_Snake_Venom_Cysteine-Rich_Sec20160513-27396-1ts0nl4.pdf","download_url":"https://www.academia.edu/attachments/45612678/download_file","bulk_download_file_name":"Crovirin_a_Snake_Venom_Cysteine_Rich_Sec.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/45612678/Crovirin_a_Snake_Venom_Cysteine-Rich_Sec20160513-27396-1ts0nl4-libre.pdf?1463201301=\u0026response-content-disposition=attachment%3B+filename%3DCrovirin_a_Snake_Venom_Cysteine_Rich_Sec.pdf\u0026Expires=1743604680\u0026Signature=hGIOLZ7EUR1RvLbgDRb~5KYmXcWBSDNW-pV13Esq2eiCKfoP4LcJAPZHSnP0lYbKneYLaCdFmyE1hGOaS2eO~6vS~hp7DiM2rXfjxwLKp1ZqgyHfmXNXYOnx~psxSbLEOGdSxJBZxRkqbL6xA6MF~P9mMlvk-VAvrNXXDoKc2w88NaQfJWGiAecKrj8GYd6Wcds3rQRi3~64KhzztktwJ~kWgNcfQxlBMkzPyrL~nvTwFZ2HVnR9lzw4QqYW3ilBrfs9XttZG9v-EMEMONHh8Jqvkg9~r~FhTd-eHtiXO49zfiTEEa~RFdoeyui7g8w6I7UPOlRr48EbfMVpCknsZA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":19849,"name":"Leishmania","url":"https://www.academia.edu/Documents/in/Leishmania"},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences"},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice"},{"id":163275,"name":"Tandem Mass Spectrometry","url":"https://www.academia.edu/Documents/in/Tandem_Mass_Spectrometry"},{"id":193464,"name":"Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis","url":"https://www.academia.edu/Documents/in/Sodium_Dodecyl_Sulfate-Polyacrylamide_Gel_Electrophoresis"},{"id":225340,"name":"Chagas disease","url":"https://www.academia.edu/Documents/in/Chagas_disease"},{"id":317322,"name":"Crotalus","url":"https://www.academia.edu/Documents/in/Crotalus"},{"id":376084,"name":"Trypanosoma Cruzi","url":"https://www.academia.edu/Documents/in/Trypanosoma_Cruzi"},{"id":1166930,"name":"Cytoplasm","url":"https://www.academia.edu/Documents/in/Cytoplasm"},{"id":1784787,"name":"Neglected diseases","url":"https://www.academia.edu/Documents/in/Neglected_diseases"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") if (false) { Aedu.setUpFigureCarousel('profile-work-13178842-figures'); } }); </script> <div class="js-work-strip profile--work_container" data-work-id="13178841"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/13178841/Trypanosoma_cruzi_Peptidases_An_Overview"><img alt="Research paper thumbnail of Trypanosoma cruzi Peptidases: An Overview" class="work-thumbnail" src="https://attachments.academia-assets.com/45612663/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/13178841/Trypanosoma_cruzi_Peptidases_An_Overview">Trypanosoma cruzi Peptidases: An Overview</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://ufrj.academia.edu/Soares">R. Soares</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/SouzaEdilma">Edilma Souza</a>, and <a class="" data-click-track="profile-work-strip-authors" href="https://ufrj.academia.edu/ThaisSoutopadr%C3%B3n">Thais Souto-padrón</a></span></div><div class="wp-workCard_item"><span>The Open Parasitology Journal</span><span>, 2010</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Peptidases are a group of enzymes which have a catalytic function that is to hydrolyze peptide bo...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Peptidases are a group of enzymes which have a catalytic function that is to hydrolyze peptide bonds of proteins. The enzymes that hydrolyze peptide bonds at the amino-or carboxy-terminus are classified as exopeptidases, and those that cleave peptide bonds inside the polypeptide are endopeptidases. Endopeptidases, such as cysteine-, metalo-, serine-and threonine peptidases as well as some exopeptidases, have been characterized in Trypanosoma cruzi. Understanding the pathogenesis of T. cruzi requires the identification of functional properties of those peptidases, as they are implied in virulence, are important for host-parasite interactions and are critical for successful survival in their hosts. Here we examine the main T. cruzi peptidases, focusing on their biological roles, especially concerning the parasite-mammalian host relations.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="dac88a0c903ae7df7f66095006d28965" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":45612663,"asset_id":13178841,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/45612663/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="13178841"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="13178841"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 13178841; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=13178841]").text(description); $(".js-view-count[data-work-id=13178841]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 13178841; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='13178841']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "dac88a0c903ae7df7f66095006d28965" } } $('.js-work-strip[data-work-id=13178841]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":13178841,"title":"Trypanosoma cruzi Peptidases: An Overview","translated_title":"","metadata":{"grobid_abstract":"Peptidases are a group of enzymes which have a catalytic function that is to hydrolyze peptide bonds of proteins. 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Adult worms present the cuticle as the interface structure between host and parasite. Cuticle structure and the demonstration of the presence of basic proteins, lipids, small amounts of terminal carbohydrate residues,</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="bb966a38e980cc7a1611fbaf14d67f1d" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":45684006,"asset_id":13178840,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/45684006/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="13178840"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="13178840"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 13178840; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=13178840]").text(description); $(".js-view-count[data-work-id=13178840]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 13178840; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='13178840']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "bb966a38e980cc7a1611fbaf14d67f1d" } } $('.js-work-strip[data-work-id=13178840]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":13178840,"title":"Ultrastructural and cytochemical aspects of the cuticle of adult Wuchereria bancrofti (Nematoda: Filarioidea)","translated_title":"","metadata":{"abstract":"Because of the practical limitations of obtaining viable adult forms of the Wuchereria bancrofti, the major species responsible for human lymphatic filariasis, only few ultrastructural studies were carried out. 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Cytoplasmic structures that correspond to el- ements of the endosomal-lysosomal (reservosome) system</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="c396cf494752171832921c0945117675" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":37971619,"asset_id":13178838,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/37971619/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="13178838"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="13178838"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 13178838; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=13178838]").text(description); $(".js-view-count[data-work-id=13178838]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 13178838; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='13178838']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "c396cf494752171832921c0945117675" } } $('.js-work-strip[data-work-id=13178838]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":13178838,"title":"Cysteine proteinase in Trypanosoma cruzi: immunocytochemical localization and involvement in parasite-host cell interaction","translated_title":"","metadata":{"abstract":"Summary A monospecific polyclonal antibody obtained against a cysteine proteinase isolated from epimastigotes of Trypanosoma cruzi was used for the immunocyto- chemical localization of the protein by electron mi- croscopy and to analyse the role played by cysteine proteinase in the process of T. cruzi-host cell interac- tion. 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This protozoan revealed se...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The fine structure of promastigotes ofLeptomonas samueli is described. This protozoan revealed several features in common with other trypanosomatids. A large membrane-bound cavity containing many vesicles was observed near the nucleus. Pinocytotic vesicles were seen arising from the membrane lining the flagellar pocket. 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Reactions were observed in the nucleus of all stages. In the kinetoplast of epimastigote and promastigote forms reactions were noted mainly at the periphery. In trypomastigotes and choanomastigotes forms, however, an intense reacion was observed thorughout the kinetoplast. Reactions were present in cytoplasmic vesicles related to protein storage in T. cruzi and in membrane-bounded peroxisome-like organelles of H. samuelpessoai, L. samueli and C. deanei. The network of filaments which forms the paraxial rod did not react. In the flagellum, reaction was noted only at the peripheral doublet microtubules. 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The network of filaments which forms the paraxial rod did not react. In the flagellum, reaction was noted only at the peripheral doublet microtubules. PTA reacts also with structures related to the junction between the flagellar and cell body membranes.","internal_url":"https://www.academia.edu/13178836/Cytochemical_Analysis_at_the_Fine_Structural_Level_of_Trypanosomatids_Stained_with_Phosphotungstic_Acid","translated_internal_url":"","created_at":"2015-06-22T10:19:15.670-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":32431858,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":1360935,"work_id":13178836,"tagging_user_id":32431858,"tagged_user_id":31729059,"co_author_invite_id":null,"email":"w***a@biof.ufrj.br","display_order":null,"name":"Wanderley de Souza","title":"Cytochemical Analysis at the Fine-Structural Level of Trypanosomatids Stained with Phosphotungstic Acid"},{"id":1360958,"work_id":13178836,"tagging_user_id":32431858,"tagged_user_id":null,"co_author_invite_id":258348,"email":"w***a@sect.rj.gov.br","display_order":null,"name":"Wanderley Souza","title":"Cytochemical Analysis at the Fine-Structural Level of Trypanosomatids Stained with Phosphotungstic Acid"},{"id":1361025,"work_id":13178836,"tagging_user_id":32431858,"tagged_user_id":32333041,"co_author_invite_id":null,"email":"w***a@gmail.com","display_order":null,"name":"Wanderley Desouza","title":"Cytochemical Analysis at the Fine-Structural Level of Trypanosomatids Stained with Phosphotungstic Acid"}],"downloadable_attachments":[],"slug":"Cytochemical_Analysis_at_the_Fine_Structural_Level_of_Trypanosomatids_Stained_with_Phosphotungstic_Acid","translated_slug":"","page_count":null,"language":"en","content_type":"Work","summary":"The ethanolic phosphotungstic acid (PTA) technic was used to detect, at the fine-structural level, basic proteins in various developmental stages of pathogenic Trypanosoma cruzi, and nonpathogenic Herpetomonas samuelpessoai, Leptomonas samueli, and Crithidia deanei, trypanosomatids. Reactions were observed in the nucleus of all stages. In the kinetoplast of epimastigote and promastigote forms reactions were noted mainly at the periphery. In trypomastigotes and choanomastigotes forms, however, an intense reacion was observed thorughout the kinetoplast. Reactions were present in cytoplasmic vesicles related to protein storage in T. cruzi and in membrane-bounded peroxisome-like organelles of H. samuelpessoai, L. samueli and C. deanei. The network of filaments which forms the paraxial rod did not react. In the flagellum, reaction was noted only at the peripheral doublet microtubules. PTA reacts also with structures related to the junction between the flagellar and cell body membranes.","owner":{"id":32431858,"first_name":"Thais","middle_initials":null,"last_name":"Souto-padrón","page_name":"ThaisSoutopadrón","domain_name":"ufrj","created_at":"2015-06-22T08:20:34.150-07:00","display_name":"Thais Souto-padrón","url":"https://ufrj.academia.edu/ThaisSoutopadr%C3%B3n"},"attachments":[],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology"},{"id":173,"name":"Zoology","url":"https://www.academia.edu/Documents/in/Zoology"},{"id":117870,"name":"Flagella","url":"https://www.academia.edu/Documents/in/Flagella"},{"id":181569,"name":"Proteins","url":"https://www.academia.edu/Documents/in/Proteins"},{"id":317484,"name":"Fine Structure Constant","url":"https://www.academia.edu/Documents/in/Fine_Structure_Constant"},{"id":317801,"name":"Cell nucleus","url":"https://www.academia.edu/Documents/in/Cell_nucleus"},{"id":376084,"name":"Trypanosoma Cruzi","url":"https://www.academia.edu/Documents/in/Trypanosoma_Cruzi"},{"id":767538,"name":"Phosphotungstic Acid","url":"https://www.academia.edu/Documents/in/Phosphotungstic_Acid"},{"id":805587,"name":"Eukaryotic microbiology","url":"https://www.academia.edu/Documents/in/Eukaryotic_microbiology"},{"id":1577822,"name":"Eukaryotic Cell","url":"https://www.academia.edu/Documents/in/Eukaryotic_Cell"},{"id":2421830,"name":"Organoids","url":"https://www.academia.edu/Documents/in/Organoids"}],"urls":[{"id":4894870,"url":"http://www.blackwell-synergy.com/doi/abs/10.1111/j.1550-7408.1979.tb04194.x"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") if (false) { Aedu.setUpFigureCarousel('profile-work-13178836-figures'); } }); </script> <div class="js-work-strip profile--work_container" data-work-id="13178835"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/13178835/Differential_collagenolytic_activity_of_Candida_albicans_isolated_from_oral_mucosa_and_dentinal_carious_lesions_of_HIV_infected_children"><img alt="Research paper thumbnail of Differential collagenolytic activity of Candida albicans isolated from oral mucosa and dentinal carious lesions of HIV-infected children" class="work-thumbnail" src="https://attachments.academia-assets.com/45612670/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/13178835/Differential_collagenolytic_activity_of_Candida_albicans_isolated_from_oral_mucosa_and_dentinal_carious_lesions_of_HIV_infected_children">Differential collagenolytic activity of Candida albicans isolated from oral mucosa and dentinal carious lesions of HIV-infected children</a></div><div class="wp-workCard_item"><span>Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology</span><span>, 2012</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Objective. The aim of this study was to compare type I collagen degradation by Candida albicans i...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Objective. The aim of this study was to compare type I collagen degradation by Candida albicans isolated from oral mucosa (M) and cavitated active dentinal caries (CAD) of HIV-infected children. Study Design. To verify the proteolytic activity, the specimens were cultivated in brain-heart infusion medium and the supernatants were incubated in the presence or absence of type I collagen at 37°C for 12 hours and analyzed using 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Intensity of the bands on the gels was assessed by densitometric analysis using a scanner and images analyzed with software from Kodak Digital Science EDAS 120. Results. Supernatants of all the C. albicans degraded type I collagen: that from M, on average, by 38.3% (SD 21.67) and that from CAD by 54% (SD 25.94; Wilcoxon test: P Ͻ .05). Predisposing factors had no association with the percentage of type I collagen degradation (Mann-Whitney test: P Ͼ .05). Conclusions. Candida albicans from different sites of the oral cavity of HIV-infected children has proteolytic activity for type I HIV-infected children are more susceptible to Candida albicans infections. This may be associated with the high prevalence of caries in these patients. Studies that investigate the possible etiologic factors for this high prevalence, such as our research, are very relevant.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="eae03a5f3124b8ef731decce0616d277" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":45612670,"asset_id":13178835,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/45612670/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="13178835"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="13178835"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 13178835; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=13178835]").text(description); $(".js-view-count[data-work-id=13178835]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 13178835; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='13178835']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "eae03a5f3124b8ef731decce0616d277" } } $('.js-work-strip[data-work-id=13178835]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":13178835,"title":"Differential collagenolytic activity of Candida albicans isolated from oral mucosa and dentinal carious lesions of HIV-infected children","translated_title":"","metadata":{"grobid_abstract":"Objective. The aim of this study was to compare type I collagen degradation by Candida albicans isolated from oral mucosa (M) and cavitated active dentinal caries (CAD) of HIV-infected children. Study Design. To verify the proteolytic activity, the specimens were cultivated in brain-heart infusion medium and the supernatants were incubated in the presence or absence of type I collagen at 37°C for 12 hours and analyzed using 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Intensity of the bands on the gels was assessed by densitometric analysis using a scanner and images analyzed with software from Kodak Digital Science EDAS 120. Results. Supernatants of all the C. albicans degraded type I collagen: that from M, on average, by 38.3% (SD 21.67) and that from CAD by 54% (SD 25.94; Wilcoxon test: P Ͻ .05). Predisposing factors had no association with the percentage of type I collagen degradation (Mann-Whitney test: P Ͼ .05). Conclusions. Candida albicans from different sites of the oral cavity of HIV-infected children has proteolytic activity for type I HIV-infected children are more susceptible to Candida albicans infections. This may be associated with the high prevalence of caries in these patients. Studies that investigate the possible etiologic factors for this high prevalence, such as our research, are very relevant.","publication_date":{"day":null,"month":null,"year":2012,"errors":{}},"publication_name":"Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology","grobid_abstract_attachment_id":45612670},"translated_abstract":null,"internal_url":"https://www.academia.edu/13178835/Differential_collagenolytic_activity_of_Candida_albicans_isolated_from_oral_mucosa_and_dentinal_carious_lesions_of_HIV_infected_children","translated_internal_url":"","created_at":"2015-06-22T10:19:15.085-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":32431858,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":1361157,"work_id":13178835,"tagging_user_id":32431858,"tagged_user_id":null,"co_author_invite_id":434361,"email":"m***a@hotmail.com","display_order":null,"name":"Maristela Portela","title":"Differential collagenolytic activity of Candida albicans isolated from oral mucosa and dentinal carious lesions of HIV-infected children"},{"id":1361162,"work_id":13178835,"tagging_user_id":32431858,"tagged_user_id":null,"co_author_invite_id":434363,"email":"r***s@praiadosol.com.br","display_order":null,"name":"Rosangela Maria Araújo Soares","title":"Differential collagenolytic activity of Candida albicans isolated from oral mucosa and dentinal carious lesions of HIV-infected children"},{"id":1361165,"work_id":13178835,"tagging_user_id":32431858,"tagged_user_id":null,"co_author_invite_id":432623,"email":"g***c@biof.ufrj.br","display_order":null,"name":"Gloria Fernanda Barbosa Araújo Castro","title":"Differential collagenolytic activity of Candida albicans isolated from oral mucosa and dentinal carious lesions of HIV-infected children"},{"id":1360972,"work_id":13178835,"tagging_user_id":32431858,"tagged_user_id":26501371,"co_author_invite_id":null,"email":"r***s@micro.ufrj.br","affiliation":"Universidade Federal do Rio de Janeiro (UFRJ)","display_order":null,"name":"Araújo Soares","title":"Differential collagenolytic activity of Candida albicans isolated from oral mucosa and dentinal carious lesions of HIV-infected children"},{"id":1361166,"work_id":13178835,"tagging_user_id":32431858,"tagged_user_id":null,"co_author_invite_id":434364,"email":"c***o@urbi.com.br","display_order":null,"name":"Gloria Castro","title":"Differential collagenolytic activity of Candida albicans isolated from oral mucosa and dentinal carious lesions of HIV-infected children"},{"id":1361001,"work_id":13178835,"tagging_user_id":32431858,"tagged_user_id":32528887,"co_author_invite_id":300859,"email":"r***s@gmail.com","affiliation":"Universidade Federal do Rio de Janeiro (UFRJ)","display_order":null,"name":"R. Soares","title":"Differential collagenolytic activity of Candida albicans isolated from oral mucosa and dentinal carious lesions of HIV-infected children"},{"id":1361164,"work_id":13178835,"tagging_user_id":32431858,"tagged_user_id":null,"co_author_invite_id":203099,"email":"d***s@dpf.gov.br","display_order":null,"name":"Madeleine Chagas","title":"Differential collagenolytic activity of Candida albicans isolated from oral mucosa and dentinal carious lesions of HIV-infected children"}],"downloadable_attachments":[{"id":45612670,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/45612670/thumbnails/1.jpg","file_name":"Differential_collagenolytic_activity_of_20160513-23640-tuzcu9.pdf","download_url":"https://www.academia.edu/attachments/45612670/download_file","bulk_download_file_name":"Differential_collagenolytic_activity_of.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/45612670/Differential_collagenolytic_activity_of_20160513-23640-tuzcu9-libre.pdf?1463201300=\u0026response-content-disposition=attachment%3B+filename%3DDifferential_collagenolytic_activity_of.pdf\u0026Expires=1743616266\u0026Signature=B5S9n8I-7qSDrgPjChKJJ~J0oQWlQhF6bM1ul4SnwAek-dDC-8XTdj34ar7fHFvUYSVNr4Y2AVi7N6QPspIhswWaMsgi9aYJ-0R2DErganuBg8cS1IoY~1T2cMKar4rk3t6ihrN-7FYGLfpyaXRqup89VPF2C9f4pe6AUKnGoKxW064u57gtgEnHLvJEG0PkBh5cXjcHhrXd-CzqpaEo0iDuAjPtWyYvcdMcHxrjyIR9ChmjnvI7tA0OdwEioXjOIQBew0YMjwiGk5zH8-I-7p6FLVzi9pK2gGnIIS2UCH6dQljHFEv4GcrJ4Q8MT1wkmNhd1hy309lGv4sOMKG9xQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Differential_collagenolytic_activity_of_Candida_albicans_isolated_from_oral_mucosa_and_dentinal_carious_lesions_of_HIV_infected_children","translated_slug":"","page_count":6,"language":"en","content_type":"Work","summary":"Objective. The aim of this study was to compare type I collagen degradation by Candida albicans isolated from oral mucosa (M) and cavitated active dentinal caries (CAD) of HIV-infected children. Study Design. To verify the proteolytic activity, the specimens were cultivated in brain-heart infusion medium and the supernatants were incubated in the presence or absence of type I collagen at 37°C for 12 hours and analyzed using 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Intensity of the bands on the gels was assessed by densitometric analysis using a scanner and images analyzed with software from Kodak Digital Science EDAS 120. Results. Supernatants of all the C. albicans degraded type I collagen: that from M, on average, by 38.3% (SD 21.67) and that from CAD by 54% (SD 25.94; Wilcoxon test: P Ͻ .05). Predisposing factors had no association with the percentage of type I collagen degradation (Mann-Whitney test: P Ͼ .05). Conclusions. Candida albicans from different sites of the oral cavity of HIV-infected children has proteolytic activity for type I HIV-infected children are more susceptible to Candida albicans infections. This may be associated with the high prevalence of caries in these patients. Studies that investigate the possible etiologic factors for this high prevalence, such as our research, are very relevant.","owner":{"id":32431858,"first_name":"Thais","middle_initials":null,"last_name":"Souto-padrón","page_name":"ThaisSoutopadrón","domain_name":"ufrj","created_at":"2015-06-22T08:20:34.150-07:00","display_name":"Thais Souto-padrón","url":"https://ufrj.academia.edu/ThaisSoutopadr%C3%B3n"},"attachments":[{"id":45612670,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/45612670/thumbnails/1.jpg","file_name":"Differential_collagenolytic_activity_of_20160513-23640-tuzcu9.pdf","download_url":"https://www.academia.edu/attachments/45612670/download_file","bulk_download_file_name":"Differential_collagenolytic_activity_of.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/45612670/Differential_collagenolytic_activity_of_20160513-23640-tuzcu9-libre.pdf?1463201300=\u0026response-content-disposition=attachment%3B+filename%3DDifferential_collagenolytic_activity_of.pdf\u0026Expires=1743616266\u0026Signature=B5S9n8I-7qSDrgPjChKJJ~J0oQWlQhF6bM1ul4SnwAek-dDC-8XTdj34ar7fHFvUYSVNr4Y2AVi7N6QPspIhswWaMsgi9aYJ-0R2DErganuBg8cS1IoY~1T2cMKar4rk3t6ihrN-7FYGLfpyaXRqup89VPF2C9f4pe6AUKnGoKxW064u57gtgEnHLvJEG0PkBh5cXjcHhrXd-CzqpaEo0iDuAjPtWyYvcdMcHxrjyIR9ChmjnvI7tA0OdwEioXjOIQBew0YMjwiGk5zH8-I-7p6FLVzi9pK2gGnIIS2UCH6dQljHFEv4GcrJ4Q8MT1wkmNhd1hy309lGv4sOMKG9xQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":3243,"name":"Nonparametric Statistics","url":"https://www.academia.edu/Documents/in/Nonparametric_Statistics"},{"id":64933,"name":"Child","url":"https://www.academia.edu/Documents/in/Child"},{"id":105826,"name":"Dental Caries","url":"https://www.academia.edu/Documents/in/Dental_Caries"},{"id":131573,"name":"Candida albicans","url":"https://www.academia.edu/Documents/in/Candida_albicans"},{"id":385863,"name":"Mouth mucosa","url":"https://www.academia.edu/Documents/in/Mouth_mucosa"},{"id":558068,"name":"Dentin","url":"https://www.academia.edu/Documents/in/Dentin"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") if (false) { Aedu.setUpFigureCarousel('profile-work-13178835-figures'); } }); </script> <div class="js-work-strip profile--work_container" data-work-id="13178834"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/13178834/Golgi_UDP_GlcNAc_polypeptide_O_%CE%B1_N_Acetyl_d_glucosaminyltransferase_2_TcOGNT2_regulates_trypomastigote_production_and_function_in_Trypanosoma_cruzi"><img alt="Research paper thumbnail of Golgi UDP-GlcNAc:polypeptide O-α-N-Acetyl-d-glucosaminyltransferase 2 (TcOGNT2) regulates trypomastigote production and function in Trypanosoma cruzi" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title">Golgi UDP-GlcNAc:polypeptide O-α-N-Acetyl-d-glucosaminyltransferase 2 (TcOGNT2) regulates trypomastigote production and function in Trypanosoma cruzi</div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://ufrj.academia.edu/ThaisSoutopadr%C3%B3n">Thais Souto-padrón</a> and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/FabricioMontalv%C3%A3o">Fabricio Montalvão</a></span></div><div class="wp-workCard_item"><span>Eukaryotic cell</span><span>, 2014</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">All life cycle stages of the protozoan parasite Trypanosoma cruzi are enveloped by mucin-like gly...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">All life cycle stages of the protozoan parasite Trypanosoma cruzi are enveloped by mucin-like glycoproteins which, despite major changes in their polypeptide cores, are extensively and similarly O-glycosylated. O-Glycan biosynthesis is initiated by the addition of αGlcNAc to Thr in a reaction catalyzed by Golgi UDP-GlcNAc:polypeptide O-α-N-acetyl-d-glucosaminyltransferases (ppαGlcNAcTs), which are encoded by TcOGNT1 and TcOGNT2. We now directly show that TcOGNT2 is associated with the Golgi apparatus of the epimastigote stage and is markedly downregulated in both differentiated metacyclic trypomastigotes (MCTs) and cell culture-derived trypomastigotes (TCTs). The significance of downregulation was examined by forced continued expression of TcOGNT2, which resulted in a substantial increase of TcOGNT2 protein levels but only modestly increased ppαGlcNAcT activity in extracts and altered cell surface glycosylation in TCTs. Constitutive TcOGNT2 overexpression had no discernible effect o...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="13178834"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="13178834"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 13178834; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=13178834]").text(description); $(".js-view-count[data-work-id=13178834]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 13178834; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='13178834']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=13178834]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":13178834,"title":"Golgi UDP-GlcNAc:polypeptide O-α-N-Acetyl-d-glucosaminyltransferase 2 (TcOGNT2) regulates trypomastigote production and function in Trypanosoma cruzi","translated_title":"","metadata":{"abstract":"All life cycle stages of the protozoan parasite Trypanosoma cruzi are enveloped by mucin-like glycoproteins which, despite major changes in their polypeptide cores, are extensively and similarly O-glycosylated. 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In this study, we evaluated whether pretreatment with SP600125 was able to prevent Orthopoxviruses Vaccinia virus (VACV), Cowpox virus (CPXV) and modified Vaccinia virus Ankara (MVA) replication. We found that incubation with SP600125 not only blocked virus-stimulated JNK phosphorylation, but also, significantly reduced</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="36f8aaef8f3e2a4397ec3bd04a009d03" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":45684233,"asset_id":13178833,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/45684233/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="13178833"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="13178833"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 13178833; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=13178833]").text(description); $(".js-view-count[data-work-id=13178833]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 13178833; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='13178833']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "36f8aaef8f3e2a4397ec3bd04a009d03" } } $('.js-work-strip[data-work-id=13178833]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":13178833,"title":"SP600125 inhibits Orthopoxviruses replication in a JNK1/2 -independent manner: Implication as a potential antipoxviral","translated_title":"","metadata":{"abstract":"The pharmacological inhibitor SP600125 [anthra(1,9-cd)pyrazol-6(2H)-one 1,9-pyrazoloanthrone] has been largely employed as a c-JUN N-terminal kinase (JNK1/2) inhibitor. 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We found that incubation with SP600125 not only blocked virus-stimulated JNK phosphorylation, but also, significantly reduced","owner":{"id":32431858,"first_name":"Thais","middle_initials":null,"last_name":"Souto-padrón","page_name":"ThaisSoutopadrón","domain_name":"ufrj","created_at":"2015-06-22T08:20:34.150-07:00","display_name":"Thais Souto-padrón","url":"https://ufrj.academia.edu/ThaisSoutopadr%C3%B3n"},"attachments":[{"id":45684233,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/45684233/thumbnails/1.jpg","file_name":"j.antiviral.2011.10.02020160516-23030-1cx44so.pdf","download_url":"https://www.academia.edu/attachments/45684233/download_file","bulk_download_file_name":"SP600125_inhibits_Orthopoxviruses_replic.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/45684233/j.antiviral.2011.10.02020160516-23030-1cx44so-libre.pdf?1463449993=\u0026response-content-disposition=attachment%3B+filename%3DSP600125_inhibits_Orthopoxviruses_replic.pdf\u0026Expires=1743616267\u0026Signature=TxlZxP5IKtvFfLUfHMo63kGUzfY6PZlctWgqkmkQedfS6rvqSVbqXxSNwZVgRCbMB-NIvy6daYqIa8mAAexV~xugWZzCBi7sd6ZPdNYB7GQ2AVeytWwg0x6KuEJ8AgKHIYiSNmTxcDtmMpMS0HjHtWE0SsS~YsWzWjPw7as9e3ZZUnc7JWOhZdUaUKGjjcKsmDCXG~ysNfTxugCxSCwY1pDdVkmYLeSwk3pYfiYTEBzfwfdSBe4H9XwwcZrrQryYGkA6PqAYTtBo5hVpEF0AqsybY-9Gk0Wa4IaWMctGSHoYvHzq9dkPQrbT6krtVed0CZjGBXk6Sbinb4HwANjeUQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":6947,"name":"Medical Microbiology","url":"https://www.academia.edu/Documents/in/Medical_Microbiology"},{"id":57570,"name":"Cercopithecus aethiops","url":"https://www.academia.edu/Documents/in/Cercopithecus_aethiops"},{"id":57808,"name":"Cell line","url":"https://www.academia.edu/Documents/in/Cell_line"},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice"},{"id":172083,"name":"Phosphorylation","url":"https://www.academia.edu/Documents/in/Phosphorylation"},{"id":213910,"name":"Mitogen Activated Protein Kinase","url":"https://www.academia.edu/Documents/in/Mitogen_Activated_Protein_Kinase"},{"id":420887,"name":"Viral Infection","url":"https://www.academia.edu/Documents/in/Viral_Infection"},{"id":561002,"name":"Vaccinia Virus","url":"https://www.academia.edu/Documents/in/Vaccinia_Virus"},{"id":584986,"name":"Cowpox virus","url":"https://www.academia.edu/Documents/in/Cowpox_virus"},{"id":792401,"name":"Antiviral","url":"https://www.academia.edu/Documents/in/Antiviral"},{"id":1292080,"name":"Vero cells","url":"https://www.academia.edu/Documents/in/Vero_cells"},{"id":1622318,"name":"Antiviral Agents","url":"https://www.academia.edu/Documents/in/Antiviral_Agents"},{"id":2212891,"name":"Orthopoxvirus","url":"https://www.academia.edu/Documents/in/Orthopoxvirus"}],"urls":[{"id":4894869,"url":"http://www.sciencedirect.com/science/article/pii/S0166354211004839"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") if (false) { Aedu.setUpFigureCarousel('profile-work-13178833-figures'); } }); </script> <div class="js-work-strip profile--work_container" data-work-id="13178832"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/13178832/Trypanosomatids_Odd_Organisms_Devastating_Diseases"><img alt="Research paper thumbnail of Trypanosomatids: Odd Organisms, Devastating Diseases" class="work-thumbnail" src="https://attachments.academia-assets.com/45612681/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/13178832/Trypanosomatids_Odd_Organisms_Devastating_Diseases">Trypanosomatids: Odd Organisms, Devastating Diseases</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://ufrj.academia.edu/ThaisSoutopadr%C3%B3n">Thais Souto-padrón</a> and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/LucianaZimmermann">Luciana Zimmermann</a></span></div><div class="wp-workCard_item"><span>The Open Parasitology Journal</span><span>, 2010</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Trypanosomatids cause many diseases in and on animals (including humans) and plants. Altogether, ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Trypanosomatids cause many diseases in and on animals (including humans) and plants. Altogether, about 37 million people are infected with Trypanosoma brucei (African sleeping sickness), Trypanosoma cruzi (Chagas disease) and Leishmania species (distinct forms of leishmaniasis worldwide). The class Kinetoplastea is divided into the subclasses Prokinetoplastina (order Prokinetoplastida) and Metakinetoplastina (orders Eubodonida, Parabodonida, Neobodonida and Trypanosomatida) [1,2]. The Prokinetoplastida, Eubodonida, Parabodonida and Neobodonida can be free-living, commensalic or parasitic; however, all members of theTrypanosomatida are parasitic. Although they seem like typical protists under the microscope the kinetoplastids have some unique features. In this review we will give an overview of the family Trypanosomatidae, with particular emphasis on some of its "peculiarities" (a single ramified mitochondrion; unusual mitochondrial DNA, the kinetoplast; a complex form of mitochondrial RNA editing; transcription of all protein-encoding genes polycistronically; trans-splicing of all mRNA transcripts; the glycolytic pathway within glycosomes; T. brucei variable surface glycoproteins and T. cruzi ability to escape from the phagocytic vacuoles), as well as the major diseases caused by members of this family. However, the present review does not cover all trypanosomatids; for example, the insect trypanosomatids are underrepresented here. On the other hand, reviews on this particular group of parasites have been written by experts in the field .</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="0fca710fac3e052916c641cbe31b7e75" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":45612681,"asset_id":13178832,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/45612681/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="13178832"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="13178832"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 13178832; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=13178832]").text(description); $(".js-view-count[data-work-id=13178832]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 13178832; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='13178832']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "0fca710fac3e052916c641cbe31b7e75" } } $('.js-work-strip[data-work-id=13178832]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":13178832,"title":"Trypanosomatids: Odd Organisms, Devastating Diseases","translated_title":"","metadata":{"grobid_abstract":"Trypanosomatids cause many diseases in and on animals (including humans) and plants. Altogether, about 37 million people are infected with Trypanosoma brucei (African sleeping sickness), Trypanosoma cruzi (Chagas disease) and Leishmania species (distinct forms of leishmaniasis worldwide). The class Kinetoplastea is divided into the subclasses Prokinetoplastina (order Prokinetoplastida) and Metakinetoplastina (orders Eubodonida, Parabodonida, Neobodonida and Trypanosomatida) [1,2]. The Prokinetoplastida, Eubodonida, Parabodonida and Neobodonida can be free-living, commensalic or parasitic; however, all members of theTrypanosomatida are parasitic. Although they seem like typical protists under the microscope the kinetoplastids have some unique features. In this review we will give an overview of the family Trypanosomatidae, with particular emphasis on some of its \"peculiarities\" (a single ramified mitochondrion; unusual mitochondrial DNA, the kinetoplast; a complex form of mitochondrial RNA editing; transcription of all protein-encoding genes polycistronically; trans-splicing of all mRNA transcripts; the glycolytic pathway within glycosomes; T. brucei variable surface glycoproteins and T. cruzi ability to escape from the phagocytic vacuoles), as well as the major diseases caused by members of this family. However, the present review does not cover all trypanosomatids; for example, the insect trypanosomatids are underrepresented here. 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Altogether, about 37 million people are infected with Trypanosoma brucei (African sleeping sickness), Trypanosoma cruzi (Chagas disease) and Leishmania species (distinct forms of leishmaniasis worldwide). The class Kinetoplastea is divided into the subclasses Prokinetoplastina (order Prokinetoplastida) and Metakinetoplastina (orders Eubodonida, Parabodonida, Neobodonida and Trypanosomatida) [1,2]. The Prokinetoplastida, Eubodonida, Parabodonida and Neobodonida can be free-living, commensalic or parasitic; however, all members of theTrypanosomatida are parasitic. Although they seem like typical protists under the microscope the kinetoplastids have some unique features. In this review we will give an overview of the family Trypanosomatidae, with particular emphasis on some of its \"peculiarities\" (a single ramified mitochondrion; unusual mitochondrial DNA, the kinetoplast; a complex form of mitochondrial RNA editing; transcription of all protein-encoding genes polycistronically; trans-splicing of all mRNA transcripts; the glycolytic pathway within glycosomes; T. brucei variable surface glycoproteins and T. cruzi ability to escape from the phagocytic vacuoles), as well as the major diseases caused by members of this family. However, the present review does not cover all trypanosomatids; for example, the insect trypanosomatids are underrepresented here. On the other hand, reviews on this particular group of parasites have been written by experts in the field .","owner":{"id":32431858,"first_name":"Thais","middle_initials":null,"last_name":"Souto-padrón","page_name":"ThaisSoutopadrón","domain_name":"ufrj","created_at":"2015-06-22T08:20:34.150-07:00","display_name":"Thais Souto-padrón","url":"https://ufrj.academia.edu/ThaisSoutopadr%C3%B3n"},"attachments":[{"id":45612681,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/45612681/thumbnails/1.jpg","file_name":"Trypanosomatids_Odd_Organisms_Devastatin20160513-19714-1b3rymb.pdf","download_url":"https://www.academia.edu/attachments/45612681/download_file","bulk_download_file_name":"Trypanosomatids_Odd_Organisms_Devastatin.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/45612681/Trypanosomatids_Odd_Organisms_Devastatin20160513-19714-1b3rymb-libre.pdf?1463201313=\u0026response-content-disposition=attachment%3B+filename%3DTrypanosomatids_Odd_Organisms_Devastatin.pdf\u0026Expires=1743616267\u0026Signature=efypOeSrLGFoko3StWy5rd6dwZyIkZxt0VJ21u-QR3kISYEiSsvZJ~wd2rD7cxGKW~TSgXCU0I085NaaeYOHrL9AV6AcElNj2CsAZN1oYJ9qi6AL1qcCHzZdmFJGbPU1nd4hYMUjrjKVBP4-PFoZ0EigeMJ0g8FfxnI-XIABNSFpfbkjEGBPZWhNLX~rVnG8QCgHVLNFyeS~vdeMVI33Zhnb5XaUEEprOVX~Z6sqex2PbWtqirIgQS7cmd4J1Eh2sR425OAnfwmR6K5YAfQOpxXkXzMCeUGaFiXB6ZR~-V1C5dFa39SMTv3IWlwku~8zvT2eAKwuz7Ft8Dp6p8HSNQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") if (false) { Aedu.setUpFigureCarousel('profile-work-13178832-figures'); } }); </script> <div class="js-work-strip profile--work_container" data-work-id="13178831"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/13178831/Transovum_Transmission_of_Trypanosomatid_Cysts_in_the_Milkweed_Bug_Oncopeltus_fasciatus"><img alt="Research paper thumbnail of Transovum Transmission of Trypanosomatid Cysts in the Milkweed Bug, Oncopeltus fasciatus" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title">Transovum Transmission of Trypanosomatid Cysts in the Milkweed Bug, Oncopeltus fasciatus</div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://ufrj.academia.edu/ThaisSoutopadr%C3%B3n">Thais Souto-padrón</a> and <a class="" data-click-track="profile-work-strip-authors" href="https://ufrj.academia.edu/LuizRicardoCostaVasconcellos">Luiz Ricardo Costa Vasconcellos</a></span></div><div class="wp-workCard_item"><span>PLoS ONE</span><span>, 2014</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Leptomonas wallacei is a trypanosomatid that develops promastigotes and cystic forms in the gut o...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Leptomonas wallacei is a trypanosomatid that develops promastigotes and cystic forms in the gut of the hemipteran insect Oncopeltus fasciatus. Insect trypanosomatids are thought to be solely transmitted from one host to another through the ingestion of parasite-contaminated feces. However, here we show that L. wallacei cysts present on the eggshells of eggs laid by O. fasciatus can also act as infective forms that are transmitted to the insect offspring. Newly hatched O. faciatus nymphs are parasite-free, but some of them become contaminated with L. wallacei after feeding on eggshell remnants. The present study is the first report of transovum transmission of a trypanosomatid, a process that may have a relevant role in parasite&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;s within-host population dynamics.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="13178831"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="13178831"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 13178831; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=13178831]").text(description); $(".js-view-count[data-work-id=13178831]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 13178831; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='13178831']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=13178831]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":13178831,"title":"Transovum Transmission of Trypanosomatid Cysts in the Milkweed Bug, Oncopeltus fasciatus","translated_title":"","metadata":{"abstract":"Leptomonas wallacei is a trypanosomatid that develops promastigotes and cystic forms in the gut of the hemipteran insect Oncopeltus fasciatus. 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The present study is the first report of transovum transmission of a trypanosomatid, a process that may have a relevant role in parasite\u0026amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;s within-host population dynamics.","publication_date":{"day":null,"month":null,"year":2014,"errors":{}},"publication_name":"PLoS ONE"},"translated_abstract":"Leptomonas wallacei is a trypanosomatid that develops promastigotes and cystic forms in the gut of the hemipteran insect Oncopeltus fasciatus. Insect trypanosomatids are thought to be solely transmitted from one host to another through the ingestion of parasite-contaminated feces. 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The present study is the first report of transovum transmission of a trypanosomatid, a process that may have a relevant role in parasite\u0026amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;s within-host population dynamics.","owner":{"id":32431858,"first_name":"Thais","middle_initials":null,"last_name":"Souto-padrón","page_name":"ThaisSoutopadrón","domain_name":"ufrj","created_at":"2015-06-22T08:20:34.150-07:00","display_name":"Thais Souto-padrón","url":"https://ufrj.academia.edu/ThaisSoutopadr%C3%B3n"},"attachments":[],"research_interests":[{"id":21953,"name":"Heteroptera","url":"https://www.academia.edu/Documents/in/Heteroptera"},{"id":28235,"name":"Multidisciplinary","url":"https://www.academia.edu/Documents/in/Multidisciplinary"},{"id":220780,"name":"PLoS one","url":"https://www.academia.edu/Documents/in/PLoS_one"},{"id":291136,"name":"Intestines","url":"https://www.academia.edu/Documents/in/Intestines"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") if (false) { Aedu.setUpFigureCarousel('profile-work-13178831-figures'); } }); </script> <div class="js-work-strip profile--work_container" data-work-id="13178829"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/13178829/Gene_expression_changes_induced_by_Trypanosoma_cruzi_shed_microvesicles_in_mammalian_host_cells_relevance_of_tRNA_derived_halves"><img alt="Research paper thumbnail of Gene expression changes induced by Trypanosoma cruzi shed microvesicles in mammalian host cells: relevance of tRNA-derived halves" class="work-thumbnail" src="https://attachments.academia-assets.com/45612715/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/13178829/Gene_expression_changes_induced_by_Trypanosoma_cruzi_shed_microvesicles_in_mammalian_host_cells_relevance_of_tRNA_derived_halves">Gene expression changes induced by Trypanosoma cruzi shed microvesicles in mammalian host cells: relevance of tRNA-derived halves</a></div><div class="wp-workCard_item"><span>BioMed research international</span><span>, 2014</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">At present, noncoding small RNAs are recognized as key players in novel forms of posttranscriptio...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">At present, noncoding small RNAs are recognized as key players in novel forms of posttranscriptional gene regulation in most eukaryotes. However, canonical small RNA pathways seem to be lost or excessively simplified in some unicellular organisms including Trypanosoma cruzi which lack functional RNAi pathways. Recently, we reported the presence of alternate small RNA pathways in T. cruzi mainly represented by homogeneous populations of tRNA- and rRNA-derived small RNAs, which are secreted to the extracellular medium included in extracellular vesicles. Extracellular vesicle cargo could be delivered to other parasites and to mammalian susceptible cells promoting metacyclogenesis and conferring susceptibility to infection, respectively. Here we analyzed the changes in gene expression of host HeLa cells induced by extracellular vesicles from T. cruzi. As assessed by microarray assays a large set of genes in HeLa cells were differentially expressed upon incorporation of T. cruzi-derived ...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="f506750d9019e17863078772c27fe400" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":45612715,"asset_id":13178829,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/45612715/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="13178829"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="13178829"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 13178829; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=13178829]").text(description); $(".js-view-count[data-work-id=13178829]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 13178829; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='13178829']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "f506750d9019e17863078772c27fe400" } } $('.js-work-strip[data-work-id=13178829]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":13178829,"title":"Gene expression changes induced by Trypanosoma cruzi shed microvesicles in mammalian host cells: relevance of tRNA-derived halves","translated_title":"","metadata":{"abstract":"At present, noncoding small RNAs are recognized as key players in novel forms of posttranscriptional gene regulation in most eukaryotes. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") if (false) { Aedu.setUpFigureCarousel('profile-work-13178829-figures'); } }); </script> </div><div class="profile--tab_content_container js-tab-pane tab-pane" data-section-id="3073828" id="papers"><div class="js-work-strip profile--work_container" data-work-id="13178851"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/13178851/Further_Studies_on_the_Organization_of_the_Paraxial_Rod_of_Trypanosomatids_1"><img alt="Research paper thumbnail of Further Studies on the Organization of the Paraxial Rod of Trypanosomatids 1" class="work-thumbnail" src="https://attachments.academia-assets.com/45684237/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/13178851/Further_Studies_on_the_Organization_of_the_Paraxial_Rod_of_Trypanosomatids_1">Further Studies on the Organization of the Paraxial Rod of Trypanosomatids 1</a></div><div class="wp-workCard_item"><span>The Journal of Protozoology</span><span>, 1986</span></div><div class="wp-workCard_item"><div class="carousel-container carousel-container--sm" id="profile-work-13178851-figures"><div class="prev-slide-container js-prev-button-container"><button aria-label="Previous" class="carousel-navigation-button js-profile-work-13178851-figures-prev"><span class="material-symbols-outlined" style="font-size: 24px" translate="no">arrow_back_ios</span></button></div><div class="slides-container js-slides-container"><figure class="figure-slide-container"><a href="https://www.academia.edu/figures/4387302/figure-1-further-studies-on-the-organization-of-the-paraxial"><img alt="" class="figure-slide-image" src="https://figures.academia-assets.com/45684237/figure_001.jpg" /></a></figure><figure class="figure-slide-container"><a href="https://www.academia.edu/figures/4387305/figure-2-farina-et-al-paraxial-rod-structure-of"><img alt="FARINA ET AL.—PARAXIAL ROD STRUCTURE OF TRYPANOSOMATIDS " class="figure-slide-image" src="https://figures.academia-assets.com/45684237/figure_002.jpg" /></a></figure></div><div class="next-slide-container js-next-button-container"><button aria-label="Next" class="carousel-navigation-button js-profile-work-13178851-figures-next"><span class="material-symbols-outlined" style="font-size: 24px" translate="no">arrow_forward_ios</span></button></div></div></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="2f727b048b982938652ef0823684de9a" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":45684237,"asset_id":13178851,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/45684237/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="13178851"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="13178851"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 13178851; 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") if (true) { Aedu.setUpFigureCarousel('profile-work-13178851-figures'); } }); </script> <div class="js-work-strip profile--work_container" data-work-id="13178850"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/13178850/Structure_and_expression_of_two_Trypanosoma_cruzi_genes_encoding_antigenic_proteins_bearing_repetitive_epitopes"><img alt="Research paper thumbnail of Structure and expression of two Trypanosoma cruzi genes encoding antigenic proteins bearing repetitive epitopes" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title">Structure and expression of two Trypanosoma cruzi genes encoding antigenic proteins bearing repetitive epitopes</div><div class="wp-workCard_item"><span>Molecular and Biochemical Parasitology</span><span>, 1989</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Trypanosoma cruzi genes were cloned in lambda gt11 and screened with an anti-trypomastigote antis...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Trypanosoma cruzi genes were cloned in lambda gt11 and screened with an anti-trypomastigote antiserum. Two out of twelve clones were selected in view of their reactivity with human chagasic sera. One clone encodes a flagellar antigen (FRA) of more than 300 kDa, whereas the other corresponds to a roughly 225-kDa cytoplasmic antigen (CRA). The flagellar antigen is present in both epimastigotes and trypomastigotes, but the cytoplasmic antigen is not found in trypomastigotes. The CRA clone is entirely composed of at least 23 copies of a 42-bp repeat and the FRA gene contains at least 14 copies of a 204-bp motif. The FRA gene hybridizes to a RNA of about 10 kb, while the CRA gene detects a transcript of 5.2 kb.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="13178850"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="13178850"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 13178850; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=13178850]").text(description); $(".js-view-count[data-work-id=13178850]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 13178850; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='13178850']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=13178850]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":13178850,"title":"Structure and expression of two Trypanosoma cruzi genes encoding antigenic proteins bearing repetitive epitopes","translated_title":"","metadata":{"abstract":"Trypanosoma cruzi genes were cloned in lambda gt11 and screened with an anti-trypomastigote antiserum. 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The FRA gene hybridizes to a RNA of about 10 kb, while the CRA gene detects a transcript of 5.2 kb.","internal_url":"https://www.academia.edu/13178850/Structure_and_expression_of_two_Trypanosoma_cruzi_genes_encoding_antigenic_proteins_bearing_repetitive_epitopes","translated_internal_url":"","created_at":"2015-06-22T10:19:21.356-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":32431858,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":1360947,"work_id":13178850,"tagging_user_id":32431858,"tagged_user_id":null,"co_author_invite_id":258348,"email":"w***a@sect.rj.gov.br","display_order":null,"name":"Wanderley Souza","title":"Structure and expression of two Trypanosoma cruzi genes encoding antigenic proteins bearing repetitive epitopes"},{"id":1360924,"work_id":13178850,"tagging_user_id":32431858,"tagged_user_id":31729059,"co_author_invite_id":null,"email":"w***a@biof.ufrj.br","display_order":null,"name":"Wanderley de Souza","title":"Structure and expression of two Trypanosoma cruzi genes encoding antigenic proteins bearing repetitive epitopes"},{"id":1361123,"work_id":13178850,"tagging_user_id":32431858,"tagged_user_id":32778051,"co_author_invite_id":133352,"email":"m***r@fiocruz.br","affiliation":"Fundação Oswaldo Cruz","display_order":null,"name":"Marco Krieger","title":"Structure and expression of two Trypanosoma cruzi genes encoding antigenic proteins bearing repetitive epitopes"},{"id":1361013,"work_id":13178850,"tagging_user_id":32431858,"tagged_user_id":32333041,"co_author_invite_id":null,"email":"w***a@gmail.com","display_order":null,"name":"Wanderley Desouza","title":"Structure and expression of two Trypanosoma cruzi genes encoding antigenic proteins bearing repetitive epitopes"},{"id":1361119,"work_id":13178850,"tagging_user_id":32431858,"tagged_user_id":28209640,"co_author_invite_id":133351,"email":"s***1@gmail.com","affiliation":"Instituto Carlos Chagas","display_order":null,"name":"Samuel Goldenberg","title":"Structure and expression of two Trypanosoma cruzi genes encoding antigenic proteins bearing repetitive epitopes"}],"downloadable_attachments":[],"slug":"Structure_and_expression_of_two_Trypanosoma_cruzi_genes_encoding_antigenic_proteins_bearing_repetitive_epitopes","translated_slug":"","page_count":null,"language":"en","content_type":"Work","summary":"Trypanosoma cruzi genes were cloned in lambda gt11 and screened with an anti-trypomastigote antiserum. Two out of twelve clones were selected in view of their reactivity with human chagasic sera. One clone encodes a flagellar antigen (FRA) of more than 300 kDa, whereas the other corresponds to a roughly 225-kDa cytoplasmic antigen (CRA). The flagellar antigen is present in both epimastigotes and trypomastigotes, but the cytoplasmic antigen is not found in trypomastigotes. The CRA clone is entirely composed of at least 23 copies of a 42-bp repeat and the FRA gene contains at least 14 copies of a 204-bp motif. 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Catalase activity, which is inhibited by 3-amino-1, 2, 4-triazole, was detected at the ultrastructural level in the matrix of the organelle by using an alkaline diaminobenzidine medium. Freeze-fracture studies showed the presence of a large number of intramembranous particles on both the P and the E faces of the membrane of the organelle. Based on these data as well as on previous observations, it is suggested that the trypanosomatids possess an organelle that can be considered to be a peroxisome.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="13178849"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="13178849"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 13178849; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=13178849]").text(description); $(".js-view-count[data-work-id=13178849]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 13178849; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='13178849']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=13178849]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":13178849,"title":"Fine structure and cytochemistry of peroxisomes (microbodies) in Leptomonas samueli","translated_title":"","metadata":{"abstract":"Leptomonas samueli possesses in its cytoplasm a membrane-bounded organelle which can reach a length of 2.8 microns and a diameter of 0.2 microns. Catalase activity, which is inhibited by 3-amino-1, 2, 4-triazole, was detected at the ultrastructural level in the matrix of the organelle by using an alkaline diaminobenzidine medium. Freeze-fracture studies showed the presence of a large number of intramembranous particles on both the P and the E faces of the membrane of the organelle. Based on these data as well as on previous observations, it is suggested that the trypanosomatids possess an organelle that can be considered to be a peroxisome.","publication_date":{"day":null,"month":null,"year":1982,"errors":{}}},"translated_abstract":"Leptomonas samueli possesses in its cytoplasm a membrane-bounded organelle which can reach a length of 2.8 microns and a diameter of 0.2 microns. Catalase activity, which is inhibited by 3-amino-1, 2, 4-triazole, was detected at the ultrastructural level in the matrix of the organelle by using an alkaline diaminobenzidine medium. Freeze-fracture studies showed the presence of a large number of intramembranous particles on both the P and the E faces of the membrane of the organelle. Based on these data as well as on previous observations, it is suggested that the trypanosomatids possess an organelle that can be considered to be a peroxisome.","internal_url":"https://www.academia.edu/13178849/Fine_structure_and_cytochemistry_of_peroxisomes_microbodies_in_Leptomonas_samueli","translated_internal_url":"","created_at":"2015-06-22T10:19:20.815-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":32431858,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":1361202,"work_id":13178849,"tagging_user_id":32431858,"tagged_user_id":31729059,"co_author_invite_id":null,"email":"w***a@biof.ufrj.br","display_order":null,"name":"Wanderley de Souza","title":"Fine structure and cytochemistry of peroxisomes (microbodies) in Leptomonas samueli"}],"downloadable_attachments":[],"slug":"Fine_structure_and_cytochemistry_of_peroxisomes_microbodies_in_Leptomonas_samueli","translated_slug":"","page_count":null,"language":"en","content_type":"Work","summary":"Leptomonas samueli possesses in its cytoplasm a membrane-bounded organelle which can reach a length of 2.8 microns and a diameter of 0.2 microns. Catalase activity, which is inhibited by 3-amino-1, 2, 4-triazole, was detected at the ultrastructural level in the matrix of the organelle by using an alkaline diaminobenzidine medium. Freeze-fracture studies showed the presence of a large number of intramembranous particles on both the P and the E faces of the membrane of the organelle. Based on these data as well as on previous observations, it is suggested that the trypanosomatids possess an organelle that can be considered to be a peroxisome.","owner":{"id":32431858,"first_name":"Thais","middle_initials":null,"last_name":"Souto-padrón","page_name":"ThaisSoutopadrón","domain_name":"ufrj","created_at":"2015-06-22T08:20:34.150-07:00","display_name":"Thais Souto-padrón","url":"https://ufrj.academia.edu/ThaisSoutopadr%C3%B3n"},"attachments":[],"research_interests":[{"id":139007,"name":"Catalase","url":"https://www.academia.edu/Documents/in/Catalase"},{"id":186234,"name":"Medical Physiology","url":"https://www.academia.edu/Documents/in/Medical_Physiology"},{"id":317484,"name":"Fine Structure Constant","url":"https://www.academia.edu/Documents/in/Fine_Structure_Constant"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") if (false) { Aedu.setUpFigureCarousel('profile-work-13178849-figures'); } }); </script> <div class="js-work-strip profile--work_container" data-work-id="13178848"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/13178848/Cellular_signaling_during_the_macrophage_invasion_by_Trypanosoma_cruzi"><img alt="Research paper thumbnail of Cellular signaling during the macrophage invasion by Trypanosoma cruzi" class="work-thumbnail" src="https://attachments.academia-assets.com/45684095/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/13178848/Cellular_signaling_during_the_macrophage_invasion_by_Trypanosoma_cruzi">Cellular signaling during the macrophage invasion by Trypanosoma cruzi</a></div><div class="wp-workCard_item"><span>Histochemistry and cell biology</span><span>, 2002</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">We have reported that protein tyrosine kinases play an important role in the invasion of Trypanos...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">We have reported that protein tyrosine kinases play an important role in the invasion of Trypanosoma cruzi into primary resident macrophages. In the present study we carry out immunofluorescence assays, using monoclonal anti-phosphotyrosine antibodies, to reveal an accumulation of tyrosine-phosphorylated residues at the site of parasite association with the macrophage surface, colocalizing with host cell F-actin-rich domains. SDS-PAGE analysis of macrophage cell line IC-21 tyrosine phosphoproteins, labeled with [(35)S] L-methionine, revealed several peptides with increased levels of phosphorylation upon interaction with the parasite. Among them, were detected bands of 140, 120, 112, 94, 73, 67, and 56 kDa that match the molecular weights of proteins described as being tyrosine phosphorylated during events that lead to actin assembly in mononuclear phagocytes. The pretreatment of IC-21 macrophages with the tyrosine kinase inhibitor tyrphostin 23 inhibited trypomastigote uptake showin...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="2eac546fea31061980c5d97a6a3f505d" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":45684095,"asset_id":13178848,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/45684095/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="13178848"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="13178848"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 13178848; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=13178848]").text(description); $(".js-view-count[data-work-id=13178848]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 13178848; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='13178848']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "2eac546fea31061980c5d97a6a3f505d" } } $('.js-work-strip[data-work-id=13178848]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":13178848,"title":"Cellular signaling during the macrophage invasion by Trypanosoma cruzi","translated_title":"","metadata":{"abstract":"We have reported that protein tyrosine kinases play an important role in the invasion of Trypanosoma cruzi into primary resident macrophages. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") if (false) { Aedu.setUpFigureCarousel('profile-work-13178848-figures'); } }); </script> <div class="js-work-strip profile--work_container" data-work-id="13178847"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/13178847/Structural_and_functional_analysis_of_a_platelet_activating_lysophosphatidylcholine_of_Trypanosoma_cruzi"><img alt="Research paper thumbnail of Structural and functional analysis of a platelet-activating lysophosphatidylcholine of Trypanosoma cruzi" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title">Structural and functional analysis of a platelet-activating lysophosphatidylcholine of Trypanosoma cruzi</div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/MauricioOliveira25">Mauricio Oliveira</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://ufmg.academia.edu/AlexandreMarques">Alexandre Marques</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://ufrj.academia.edu/ThaisSoutopadr%C3%B3n">Thais Souto-padrón</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://utep.academia.edu/IAlmeida">Igor C Almeida</a>, and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/MartaGomes18">Marta Gomes</a></span></div><div class="wp-workCard_item"><span>PLoS neglected tropical diseases</span><span>, 2014</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Trypanosoma cruzi is the causative agent of the life-threatening Chagas disease, in which increas...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Trypanosoma cruzi is the causative agent of the life-threatening Chagas disease, in which increased platelet aggregation related to myocarditis is observed. Platelet-activating factor (PAF) is a potent intercellular lipid mediator and second messenger that exerts its activity through a PAF-specific receptor (PAFR). Previous data from our group suggested that T. cruzi synthesizes a phospholipid with PAF-like activity. The structure of T. cruzi PAF-like molecule, however, remains elusive. Here, we have purified and structurally characterized the putative T. cruzi PAF-like molecule by electrospray ionization-tandem mass spectrometry (ESI-MS/MS). Our ESI-MS/MS data demonstrated that the T. cruzi PAF-like molecule is actually a lysophosphatidylcholine (LPC), namely sn-1 C18:1(delta 9)-LPC. Similar to PAF, the platelet-aggregating activity of C18:1-LPC was abrogated by the PAFR antagonist, WEB 2086. Other major LPC species, i.e., C16:0-, C18:0-, and C18:2-LPC, were also characterized in a...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="13178847"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="13178847"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 13178847; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=13178847]").text(description); $(".js-view-count[data-work-id=13178847]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 13178847; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='13178847']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=13178847]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":13178847,"title":"Structural and functional analysis of a platelet-activating lysophosphatidylcholine of Trypanosoma cruzi","translated_title":"","metadata":{"abstract":"Trypanosoma cruzi is the causative agent of the life-threatening Chagas disease, in which increased platelet aggregation related to myocarditis is observed. Platelet-activating factor (PAF) is a potent intercellular lipid mediator and second messenger that exerts its activity through a PAF-specific receptor (PAFR). Previous data from our group suggested that T. cruzi synthesizes a phospholipid with PAF-like activity. The structure of T. cruzi PAF-like molecule, however, remains elusive. Here, we have purified and structurally characterized the putative T. cruzi PAF-like molecule by electrospray ionization-tandem mass spectrometry (ESI-MS/MS). Our ESI-MS/MS data demonstrated that the T. cruzi PAF-like molecule is actually a lysophosphatidylcholine (LPC), namely sn-1 C18:1(delta 9)-LPC. Similar to PAF, the platelet-aggregating activity of C18:1-LPC was abrogated by the PAFR antagonist, WEB 2086. 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Platelet-activating factor (PAF) is a potent intercellular lipid mediator and second messenger that exerts its activity through a PAF-specific receptor (PAFR). Previous data from our group suggested that T. cruzi synthesizes a phospholipid with PAF-like activity. The structure of T. cruzi PAF-like molecule, however, remains elusive. Here, we have purified and structurally characterized the putative T. cruzi PAF-like molecule by electrospray ionization-tandem mass spectrometry (ESI-MS/MS). Our ESI-MS/MS data demonstrated that the T. cruzi PAF-like molecule is actually a lysophosphatidylcholine (LPC), namely sn-1 C18:1(delta 9)-LPC. Similar to PAF, the platelet-aggregating activity of C18:1-LPC was abrogated by the PAFR antagonist, WEB 2086. Other major LPC species, i.e., C16:0-, C18:0-, and C18:2-LPC, were also characterized in a...","owner":{"id":32431858,"first_name":"Thais","middle_initials":null,"last_name":"Souto-padrón","page_name":"ThaisSoutopadrón","domain_name":"ufrj","created_at":"2015-06-22T08:20:34.150-07:00","display_name":"Thais Souto-padrón","url":"https://ufrj.academia.edu/ThaisSoutopadr%C3%B3n"},"attachments":[],"research_interests":[{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences"},{"id":65641,"name":"Platelet aggregation","url":"https://www.academia.edu/Documents/in/Platelet_aggregation"},{"id":86313,"name":"G protein-coupled receptors","url":"https://www.academia.edu/Documents/in/G_protein-coupled_receptors"},{"id":163275,"name":"Tandem Mass Spectrometry","url":"https://www.academia.edu/Documents/in/Tandem_Mass_Spectrometry"},{"id":326380,"name":"Liquid Chromatography / Electrospray Ionization Mass Spectrometry","url":"https://www.academia.edu/Documents/in/Liquid_Chromatography_Electrospray_Ionization_Mass_Spectrometry"},{"id":376084,"name":"Trypanosoma Cruzi","url":"https://www.academia.edu/Documents/in/Trypanosoma_Cruzi"},{"id":788677,"name":"Rabbits","url":"https://www.academia.edu/Documents/in/Rabbits"},{"id":801411,"name":"Triazoles","url":"https://www.academia.edu/Documents/in/Triazoles"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") if (false) { Aedu.setUpFigureCarousel('profile-work-13178847-figures'); } }); </script> <div class="js-work-strip profile--work_container" data-work-id="13178845"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/13178845/The_effect_of_tunicamycin_and_monensin_on_the_association_of_Trypanosoma_cruzi_with_resident_macrophages"><img alt="Research paper thumbnail of The effect of tunicamycin and monensin on the association of Trypanosoma cruzi with resident macrophages" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title">The effect of tunicamycin and monensin on the association of Trypanosoma cruzi with resident macrophages</div><div class="wp-workCard_item"><span>Parasitology Research</span><span>, 1989</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The effect of incubation of parasites (epimastigote and trypomastigote forms ofTrypanosoma cruzi)...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The effect of incubation of parasites (epimastigote and trypomastigote forms ofTrypanosoma cruzi) or macrophages in the presence of tunicamycin (TM) or monensin (M) on the parasite-macrophage association was analysed. Treatment of the parasites with TM, a drug which interferes with the process ofN-glycosylation of proteins, increased by about 70% and decreased by about 27% the infection of epimastigote and trypomastigote</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="13178845"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="13178845"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 13178845; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=13178845]").text(description); $(".js-view-count[data-work-id=13178845]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 13178845; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='13178845']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=13178845]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":13178845,"title":"The effect of tunicamycin and monensin on the association of Trypanosoma cruzi with resident macrophages","translated_title":"","metadata":{"abstract":"The effect of incubation of parasites (epimastigote and trypomastigote forms ofTrypanosoma cruzi) or macrophages in the presence of tunicamycin (TM) or monensin (M) on the parasite-macrophage association was analysed. Treatment of the parasites with TM, a drug which interferes with the process ofN-glycosylation of proteins, increased by about 70% and decreased by about 27% the infection of epimastigote and trypomastigote","publication_date":{"day":null,"month":null,"year":1989,"errors":{}},"publication_name":"Parasitology Research"},"translated_abstract":"The effect of incubation of parasites (epimastigote and trypomastigote forms ofTrypanosoma cruzi) or macrophages in the presence of tunicamycin (TM) or monensin (M) on the parasite-macrophage association was analysed. Treatment of the parasites with TM, a drug which interferes with the process ofN-glycosylation of proteins, increased by about 70% and decreased by about 27% the infection of epimastigote and trypomastigote","internal_url":"https://www.academia.edu/13178845/The_effect_of_tunicamycin_and_monensin_on_the_association_of_Trypanosoma_cruzi_with_resident_macrophages","translated_internal_url":"","created_at":"2015-06-22T10:19:19.319-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":32431858,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":1361024,"work_id":13178845,"tagging_user_id":32431858,"tagged_user_id":32333041,"co_author_invite_id":null,"email":"w***a@gmail.com","display_order":null,"name":"Wanderley Desouza","title":"The effect of tunicamycin and monensin on the association of Trypanosoma cruzi with resident macrophages"}],"downloadable_attachments":[],"slug":"The_effect_of_tunicamycin_and_monensin_on_the_association_of_Trypanosoma_cruzi_with_resident_macrophages","translated_slug":"","page_count":null,"language":"en","content_type":"Work","summary":"The effect of incubation of parasites (epimastigote and trypomastigote forms ofTrypanosoma cruzi) or macrophages in the presence of tunicamycin (TM) or monensin (M) on the parasite-macrophage association was analysed. Treatment of the parasites with TM, a drug which interferes with the process ofN-glycosylation of proteins, increased by about 70% and decreased by about 27% the infection of epimastigote and trypomastigote","owner":{"id":32431858,"first_name":"Thais","middle_initials":null,"last_name":"Souto-padrón","page_name":"ThaisSoutopadrón","domain_name":"ufrj","created_at":"2015-06-22T08:20:34.150-07:00","display_name":"Thais Souto-padrón","url":"https://ufrj.academia.edu/ThaisSoutopadr%C3%B3n"},"attachments":[],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology"},{"id":164,"name":"Parasitology","url":"https://www.academia.edu/Documents/in/Parasitology"},{"id":2184,"name":"Electron Microscopy","url":"https://www.academia.edu/Documents/in/Electron_Microscopy"},{"id":6947,"name":"Medical Microbiology","url":"https://www.academia.edu/Documents/in/Medical_Microbiology"},{"id":11558,"name":"Drug interactions","url":"https://www.academia.edu/Documents/in/Drug_interactions"},{"id":17491,"name":"Macrophages","url":"https://www.academia.edu/Documents/in/Macrophages"},{"id":90156,"name":"Endocytosis","url":"https://www.academia.edu/Documents/in/Endocytosis"},{"id":134418,"name":"Tunicamycin","url":"https://www.academia.edu/Documents/in/Tunicamycin"},{"id":167158,"name":"Trypsin","url":"https://www.academia.edu/Documents/in/Trypsin"},{"id":376084,"name":"Trypanosoma Cruzi","url":"https://www.academia.edu/Documents/in/Trypanosoma_Cruzi"},{"id":644860,"name":"Veterinary Sciences","url":"https://www.academia.edu/Documents/in/Veterinary_Sciences"},{"id":900881,"name":"Membrane Protein","url":"https://www.academia.edu/Documents/in/Membrane_Protein"},{"id":979301,"name":"Golgi Apparatus","url":"https://www.academia.edu/Documents/in/Golgi_Apparatus"},{"id":2428330,"name":"Vacuoles","url":"https://www.academia.edu/Documents/in/Vacuoles"}],"urls":[{"id":4894876,"url":"http://www.springerlink.com/index/u78k728743412t30.pdf"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") if (false) { Aedu.setUpFigureCarousel('profile-work-13178845-figures'); } }); </script> <div class="js-work-strip profile--work_container" data-work-id="13178844"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/13178844/Localization_of_lectin_binding_sites_on_the_surface_of_Trypanosoma_cruzi_grown_in_chemically_defined_conditions"><img alt="Research paper thumbnail of Localization of lectin-binding sites on the surface of Trypanosoma cruzi grown in chemically defined conditions" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title">Localization of lectin-binding sites on the surface of Trypanosoma cruzi grown in chemically defined conditions</div><div class="wp-workCard_item"><span>Histochemistry and Cell Biology</span><span>, 1998</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The transformation of Trypanosoma cruzi epimastigotes to mammal-infective metacyclic trypomastigo...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The transformation of Trypanosoma cruzi epimastigotes to mammal-infective metacyclic trypomastigotes (metacyclogenesis) can be performed in vitro under chemically defined conditions (TAU 3AAG medium). During this process, changes in the nature of cell surface sugar composition and sugar distribution was evaluated using FITC and gold-labeled lectins and observed by flow cytometry and transmission electron microscopy. The pattern of labeling with the</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="13178844"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="13178844"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 13178844; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=13178844]").text(description); $(".js-view-count[data-work-id=13178844]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 13178844; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='13178844']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=13178844]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":13178844,"title":"Localization of lectin-binding sites on the surface of Trypanosoma cruzi grown in chemically defined conditions","translated_title":"","metadata":{"abstract":"The transformation of Trypanosoma cruzi epimastigotes to mammal-infective metacyclic trypomastigotes (metacyclogenesis) can be performed in vitro under chemically defined conditions (TAU 3AAG medium). During this process, changes in the nature of cell surface sugar composition and sugar distribution was evaluated using FITC and gold-labeled lectins and observed by flow cytometry and transmission electron microscopy. The pattern of labeling with the","publication_date":{"day":null,"month":null,"year":1998,"errors":{}},"publication_name":"Histochemistry and Cell Biology"},"translated_abstract":"The transformation of Trypanosoma cruzi epimastigotes to mammal-infective metacyclic trypomastigotes (metacyclogenesis) can be performed in vitro under chemically defined conditions (TAU 3AAG medium). During this process, changes in the nature of cell surface sugar composition and sugar distribution was evaluated using FITC and gold-labeled lectins and observed by flow cytometry and transmission electron microscopy. The pattern of labeling with the","internal_url":"https://www.academia.edu/13178844/Localization_of_lectin_binding_sites_on_the_surface_of_Trypanosoma_cruzi_grown_in_chemically_defined_conditions","translated_internal_url":"","created_at":"2015-06-22T10:19:18.841-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":32431858,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":1360939,"work_id":13178844,"tagging_user_id":32431858,"tagged_user_id":31729059,"co_author_invite_id":null,"email":"w***a@biof.ufrj.br","display_order":null,"name":"Wanderley de Souza","title":"Localization of lectin-binding sites on the surface of Trypanosoma cruzi grown in chemically defined conditions"},{"id":1361029,"work_id":13178844,"tagging_user_id":32431858,"tagged_user_id":32333041,"co_author_invite_id":null,"email":"w***a@gmail.com","display_order":null,"name":"Wanderley Desouza","title":"Localization of lectin-binding sites on the surface of Trypanosoma cruzi grown in chemically defined conditions"},{"id":1360962,"work_id":13178844,"tagging_user_id":32431858,"tagged_user_id":null,"co_author_invite_id":258348,"email":"w***a@sect.rj.gov.br","display_order":null,"name":"Wanderley Souza","title":"Localization of lectin-binding sites on the surface of Trypanosoma cruzi grown in chemically defined conditions"}],"downloadable_attachments":[],"slug":"Localization_of_lectin_binding_sites_on_the_surface_of_Trypanosoma_cruzi_grown_in_chemically_defined_conditions","translated_slug":"","page_count":null,"language":"en","content_type":"Work","summary":"The transformation of Trypanosoma cruzi epimastigotes to mammal-infective metacyclic trypomastigotes (metacyclogenesis) can be performed in vitro under chemically defined conditions (TAU 3AAG medium). During this process, changes in the nature of cell surface sugar composition and sugar distribution was evaluated using FITC and gold-labeled lectins and observed by flow cytometry and transmission electron microscopy. The pattern of labeling with the","owner":{"id":32431858,"first_name":"Thais","middle_initials":null,"last_name":"Souto-padrón","page_name":"ThaisSoutopadrón","domain_name":"ufrj","created_at":"2015-06-22T08:20:34.150-07:00","display_name":"Thais Souto-padrón","url":"https://ufrj.academia.edu/ThaisSoutopadr%C3%B3n"},"attachments":[],"research_interests":[{"id":6599,"name":"Flow Cytometry","url":"https://www.academia.edu/Documents/in/Flow_Cytometry"},{"id":14076,"name":"Transmission Electron Microscopy","url":"https://www.academia.edu/Documents/in/Transmission_Electron_Microscopy"},{"id":138998,"name":"Lectins","url":"https://www.academia.edu/Documents/in/Lectins"},{"id":376084,"name":"Trypanosoma Cruzi","url":"https://www.academia.edu/Documents/in/Trypanosoma_Cruzi"},{"id":957359,"name":"Culture Media","url":"https://www.academia.edu/Documents/in/Culture_Media"},{"id":1078292,"name":"Cell Surface Markers","url":"https://www.academia.edu/Documents/in/Cell_Surface_Markers"},{"id":1146416,"name":"Arachis Hypogaea","url":"https://www.academia.edu/Documents/in/Arachis_Hypogaea"},{"id":1242506,"name":"Binding Site","url":"https://www.academia.edu/Documents/in/Binding_Site"}],"urls":[{"id":4894875,"url":"http://www.springerlink.com/index/lq1pe7dqr5kctm3q.pdf"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") if (false) { Aedu.setUpFigureCarousel('profile-work-13178844-figures'); } }); </script> <div class="js-work-strip profile--work_container" data-work-id="13178843"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/13178843/Acetylated_alpha_tubulin_in_Trypanosoma_cruzi_immunocytochemical_localization"><img alt="Research paper thumbnail of Acetylated alpha-tubulin in Trypanosoma cruzi: immunocytochemical localization" class="work-thumbnail" src="https://attachments.academia-assets.com/45612680/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/13178843/Acetylated_alpha_tubulin_in_Trypanosoma_cruzi_immunocytochemical_localization">Acetylated alpha-tubulin in Trypanosoma cruzi: immunocytochemical localization</a></div><div class="wp-workCard_item"><span>Memorias Do Instituto Oswaldo Cruz</span><span>, 1993</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="c6778f016f27745bbd1b4529d698d062" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" 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href="https://www.academia.edu/13178842/Crovirin_a_Snake_Venom_Cysteine_Rich_Secretory_Protein_CRISP_with_Promising_Activity_against_Trypanosomes_and_Leishmania"><img alt="Research paper thumbnail of Crovirin, a Snake Venom Cysteine-Rich Secretory Protein (CRISP) with Promising Activity against Trypanosomes and Leishmania" class="work-thumbnail" src="https://attachments.academia-assets.com/45612678/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/13178842/Crovirin_a_Snake_Venom_Cysteine_Rich_Secretory_Protein_CRISP_with_Promising_Activity_against_Trypanosomes_and_Leishmania">Crovirin, a Snake Venom Cysteine-Rich Secretory Protein (CRISP) with Promising Activity against Trypanosomes and Leishmania</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/JRodrigues4">J. Rodrigues</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/CamilaAdade">Camila Adade</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://ufrj.academia.edu/ThaisSoutopadr%C3%B3n">Thais Souto-padrón</a>, and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/AnaLima74">Ana Lima</a></span></div><div class="wp-workCard_item"><span>PLoS Neglected Tropical Diseases</span><span>, 2014</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Background: The neglected human diseases caused by trypanosomatids are currently treated with tox...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Background: The neglected human diseases caused by trypanosomatids are currently treated with toxic therapy with limited efficacy. In search for novel anti-trypanosomatid agents, we showed previously that the Crotalus viridis viridis (Cvv) snake venom was active against infective forms of Trypanosoma cruzi. Here, we describe the purification of crovirin, a cysteine-rich secretory protein (CRISP) from Cvv venom with promising activity against trypanosomes and Leishmania.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="5e405c9da34f38f02bd5a9282e49e1f3" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":45612678,"asset_id":13178842,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/45612678/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="13178842"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="13178842"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 13178842; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=13178842]").text(description); $(".js-view-count[data-work-id=13178842]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 13178842; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='13178842']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "5e405c9da34f38f02bd5a9282e49e1f3" } } $('.js-work-strip[data-work-id=13178842]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":13178842,"title":"Crovirin, a Snake Venom Cysteine-Rich Secretory Protein (CRISP) with Promising Activity against Trypanosomes and Leishmania","translated_title":"","metadata":{"grobid_abstract":"Background: The neglected human diseases caused by trypanosomatids are currently treated with toxic therapy with limited efficacy. 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Here, we describe the purification of crovirin, a cysteine-rich secretory protein (CRISP) from Cvv venom with promising activity against trypanosomes and Leishmania.","owner":{"id":32431858,"first_name":"Thais","middle_initials":null,"last_name":"Souto-padrón","page_name":"ThaisSoutopadrón","domain_name":"ufrj","created_at":"2015-06-22T08:20:34.150-07:00","display_name":"Thais Souto-padrón","url":"https://ufrj.academia.edu/ThaisSoutopadr%C3%B3n"},"attachments":[{"id":45612678,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/45612678/thumbnails/1.jpg","file_name":"Crovirin_a_Snake_Venom_Cysteine-Rich_Sec20160513-27396-1ts0nl4.pdf","download_url":"https://www.academia.edu/attachments/45612678/download_file","bulk_download_file_name":"Crovirin_a_Snake_Venom_Cysteine_Rich_Sec.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/45612678/Crovirin_a_Snake_Venom_Cysteine-Rich_Sec20160513-27396-1ts0nl4-libre.pdf?1463201301=\u0026response-content-disposition=attachment%3B+filename%3DCrovirin_a_Snake_Venom_Cysteine_Rich_Sec.pdf\u0026Expires=1743604680\u0026Signature=hGIOLZ7EUR1RvLbgDRb~5KYmXcWBSDNW-pV13Esq2eiCKfoP4LcJAPZHSnP0lYbKneYLaCdFmyE1hGOaS2eO~6vS~hp7DiM2rXfjxwLKp1ZqgyHfmXNXYOnx~psxSbLEOGdSxJBZxRkqbL6xA6MF~P9mMlvk-VAvrNXXDoKc2w88NaQfJWGiAecKrj8GYd6Wcds3rQRi3~64KhzztktwJ~kWgNcfQxlBMkzPyrL~nvTwFZ2HVnR9lzw4QqYW3ilBrfs9XttZG9v-EMEMONHh8Jqvkg9~r~FhTd-eHtiXO49zfiTEEa~RFdoeyui7g8w6I7UPOlRr48EbfMVpCknsZA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":19849,"name":"Leishmania","url":"https://www.academia.edu/Documents/in/Leishmania"},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences"},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice"},{"id":163275,"name":"Tandem Mass Spectrometry","url":"https://www.academia.edu/Documents/in/Tandem_Mass_Spectrometry"},{"id":193464,"name":"Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis","url":"https://www.academia.edu/Documents/in/Sodium_Dodecyl_Sulfate-Polyacrylamide_Gel_Electrophoresis"},{"id":225340,"name":"Chagas disease","url":"https://www.academia.edu/Documents/in/Chagas_disease"},{"id":317322,"name":"Crotalus","url":"https://www.academia.edu/Documents/in/Crotalus"},{"id":376084,"name":"Trypanosoma Cruzi","url":"https://www.academia.edu/Documents/in/Trypanosoma_Cruzi"},{"id":1166930,"name":"Cytoplasm","url":"https://www.academia.edu/Documents/in/Cytoplasm"},{"id":1784787,"name":"Neglected diseases","url":"https://www.academia.edu/Documents/in/Neglected_diseases"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") if (false) { Aedu.setUpFigureCarousel('profile-work-13178842-figures'); } }); </script> <div class="js-work-strip profile--work_container" data-work-id="13178841"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/13178841/Trypanosoma_cruzi_Peptidases_An_Overview"><img alt="Research paper thumbnail of Trypanosoma cruzi Peptidases: An Overview" class="work-thumbnail" src="https://attachments.academia-assets.com/45612663/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/13178841/Trypanosoma_cruzi_Peptidases_An_Overview">Trypanosoma cruzi Peptidases: An Overview</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://ufrj.academia.edu/Soares">R. Soares</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/SouzaEdilma">Edilma Souza</a>, and <a class="" data-click-track="profile-work-strip-authors" href="https://ufrj.academia.edu/ThaisSoutopadr%C3%B3n">Thais Souto-padrón</a></span></div><div class="wp-workCard_item"><span>The Open Parasitology Journal</span><span>, 2010</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Peptidases are a group of enzymes which have a catalytic function that is to hydrolyze peptide bo...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Peptidases are a group of enzymes which have a catalytic function that is to hydrolyze peptide bonds of proteins. The enzymes that hydrolyze peptide bonds at the amino-or carboxy-terminus are classified as exopeptidases, and those that cleave peptide bonds inside the polypeptide are endopeptidases. Endopeptidases, such as cysteine-, metalo-, serine-and threonine peptidases as well as some exopeptidases, have been characterized in Trypanosoma cruzi. Understanding the pathogenesis of T. cruzi requires the identification of functional properties of those peptidases, as they are implied in virulence, are important for host-parasite interactions and are critical for successful survival in their hosts. Here we examine the main T. cruzi peptidases, focusing on their biological roles, especially concerning the parasite-mammalian host relations.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="dac88a0c903ae7df7f66095006d28965" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":45612663,"asset_id":13178841,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/45612663/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="13178841"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="13178841"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 13178841; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=13178841]").text(description); $(".js-view-count[data-work-id=13178841]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 13178841; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='13178841']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "dac88a0c903ae7df7f66095006d28965" } } $('.js-work-strip[data-work-id=13178841]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":13178841,"title":"Trypanosoma cruzi Peptidases: An Overview","translated_title":"","metadata":{"grobid_abstract":"Peptidases are a group of enzymes which have a catalytic function that is to hydrolyze peptide bonds of proteins. 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Adult worms present the cuticle as the interface structure between host and parasite. Cuticle structure and the demonstration of the presence of basic proteins, lipids, small amounts of terminal carbohydrate residues,</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="bb966a38e980cc7a1611fbaf14d67f1d" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":45684006,"asset_id":13178840,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/45684006/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="13178840"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="13178840"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 13178840; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=13178840]").text(description); $(".js-view-count[data-work-id=13178840]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 13178840; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='13178840']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "bb966a38e980cc7a1611fbaf14d67f1d" } } $('.js-work-strip[data-work-id=13178840]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":13178840,"title":"Ultrastructural and cytochemical aspects of the cuticle of adult Wuchereria bancrofti (Nematoda: Filarioidea)","translated_title":"","metadata":{"abstract":"Because of the practical limitations of obtaining viable adult forms of the Wuchereria bancrofti, the major species responsible for human lymphatic filariasis, only few ultrastructural studies were carried out. 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Cytoplasmic structures that correspond to el- ements of the endosomal-lysosomal (reservosome) system</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="c396cf494752171832921c0945117675" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":37971619,"asset_id":13178838,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/37971619/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="13178838"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="13178838"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 13178838; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=13178838]").text(description); $(".js-view-count[data-work-id=13178838]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 13178838; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='13178838']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "c396cf494752171832921c0945117675" } } $('.js-work-strip[data-work-id=13178838]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":13178838,"title":"Cysteine proteinase in Trypanosoma cruzi: immunocytochemical localization and involvement in parasite-host cell interaction","translated_title":"","metadata":{"abstract":"Summary A monospecific polyclonal antibody obtained against a cysteine proteinase isolated from epimastigotes of Trypanosoma cruzi was used for the immunocyto- chemical localization of the protein by electron mi- croscopy and to analyse the role played by cysteine proteinase in the process of T. cruzi-host cell interac- tion. 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This protozoan revealed se...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The fine structure of promastigotes ofLeptomonas samueli is described. This protozoan revealed several features in common with other trypanosomatids. A large membrane-bound cavity containing many vesicles was observed near the nucleus. Pinocytotic vesicles were seen arising from the membrane lining the flagellar pocket. 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Reactions were observed in the nucleus of all stages. In the kinetoplast of epimastigote and promastigote forms reactions were noted mainly at the periphery. In trypomastigotes and choanomastigotes forms, however, an intense reacion was observed thorughout the kinetoplast. Reactions were present in cytoplasmic vesicles related to protein storage in T. cruzi and in membrane-bounded peroxisome-like organelles of H. samuelpessoai, L. samueli and C. deanei. The network of filaments which forms the paraxial rod did not react. In the flagellum, reaction was noted only at the peripheral doublet microtubules. 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The network of filaments which forms the paraxial rod did not react. In the flagellum, reaction was noted only at the peripheral doublet microtubules. PTA reacts also with structures related to the junction between the flagellar and cell body membranes.","internal_url":"https://www.academia.edu/13178836/Cytochemical_Analysis_at_the_Fine_Structural_Level_of_Trypanosomatids_Stained_with_Phosphotungstic_Acid","translated_internal_url":"","created_at":"2015-06-22T10:19:15.670-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":32431858,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":1360935,"work_id":13178836,"tagging_user_id":32431858,"tagged_user_id":31729059,"co_author_invite_id":null,"email":"w***a@biof.ufrj.br","display_order":null,"name":"Wanderley de Souza","title":"Cytochemical Analysis at the Fine-Structural Level of Trypanosomatids Stained with Phosphotungstic Acid"},{"id":1360958,"work_id":13178836,"tagging_user_id":32431858,"tagged_user_id":null,"co_author_invite_id":258348,"email":"w***a@sect.rj.gov.br","display_order":null,"name":"Wanderley Souza","title":"Cytochemical Analysis at the Fine-Structural Level of Trypanosomatids Stained with Phosphotungstic Acid"},{"id":1361025,"work_id":13178836,"tagging_user_id":32431858,"tagged_user_id":32333041,"co_author_invite_id":null,"email":"w***a@gmail.com","display_order":null,"name":"Wanderley Desouza","title":"Cytochemical Analysis at the Fine-Structural Level of Trypanosomatids Stained with Phosphotungstic Acid"}],"downloadable_attachments":[],"slug":"Cytochemical_Analysis_at_the_Fine_Structural_Level_of_Trypanosomatids_Stained_with_Phosphotungstic_Acid","translated_slug":"","page_count":null,"language":"en","content_type":"Work","summary":"The ethanolic phosphotungstic acid (PTA) technic was used to detect, at the fine-structural level, basic proteins in various developmental stages of pathogenic Trypanosoma cruzi, and nonpathogenic Herpetomonas samuelpessoai, Leptomonas samueli, and Crithidia deanei, trypanosomatids. Reactions were observed in the nucleus of all stages. In the kinetoplast of epimastigote and promastigote forms reactions were noted mainly at the periphery. In trypomastigotes and choanomastigotes forms, however, an intense reacion was observed thorughout the kinetoplast. Reactions were present in cytoplasmic vesicles related to protein storage in T. cruzi and in membrane-bounded peroxisome-like organelles of H. samuelpessoai, L. samueli and C. deanei. The network of filaments which forms the paraxial rod did not react. In the flagellum, reaction was noted only at the peripheral doublet microtubules. PTA reacts also with structures related to the junction between the flagellar and cell body membranes.","owner":{"id":32431858,"first_name":"Thais","middle_initials":null,"last_name":"Souto-padrón","page_name":"ThaisSoutopadrón","domain_name":"ufrj","created_at":"2015-06-22T08:20:34.150-07:00","display_name":"Thais Souto-padrón","url":"https://ufrj.academia.edu/ThaisSoutopadr%C3%B3n"},"attachments":[],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology"},{"id":173,"name":"Zoology","url":"https://www.academia.edu/Documents/in/Zoology"},{"id":117870,"name":"Flagella","url":"https://www.academia.edu/Documents/in/Flagella"},{"id":181569,"name":"Proteins","url":"https://www.academia.edu/Documents/in/Proteins"},{"id":317484,"name":"Fine Structure Constant","url":"https://www.academia.edu/Documents/in/Fine_Structure_Constant"},{"id":317801,"name":"Cell nucleus","url":"https://www.academia.edu/Documents/in/Cell_nucleus"},{"id":376084,"name":"Trypanosoma Cruzi","url":"https://www.academia.edu/Documents/in/Trypanosoma_Cruzi"},{"id":767538,"name":"Phosphotungstic Acid","url":"https://www.academia.edu/Documents/in/Phosphotungstic_Acid"},{"id":805587,"name":"Eukaryotic microbiology","url":"https://www.academia.edu/Documents/in/Eukaryotic_microbiology"},{"id":1577822,"name":"Eukaryotic Cell","url":"https://www.academia.edu/Documents/in/Eukaryotic_Cell"},{"id":2421830,"name":"Organoids","url":"https://www.academia.edu/Documents/in/Organoids"}],"urls":[{"id":4894870,"url":"http://www.blackwell-synergy.com/doi/abs/10.1111/j.1550-7408.1979.tb04194.x"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") if (false) { Aedu.setUpFigureCarousel('profile-work-13178836-figures'); } }); </script> <div class="js-work-strip profile--work_container" data-work-id="13178835"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/13178835/Differential_collagenolytic_activity_of_Candida_albicans_isolated_from_oral_mucosa_and_dentinal_carious_lesions_of_HIV_infected_children"><img alt="Research paper thumbnail of Differential collagenolytic activity of Candida albicans isolated from oral mucosa and dentinal carious lesions of HIV-infected children" class="work-thumbnail" src="https://attachments.academia-assets.com/45612670/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/13178835/Differential_collagenolytic_activity_of_Candida_albicans_isolated_from_oral_mucosa_and_dentinal_carious_lesions_of_HIV_infected_children">Differential collagenolytic activity of Candida albicans isolated from oral mucosa and dentinal carious lesions of HIV-infected children</a></div><div class="wp-workCard_item"><span>Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology</span><span>, 2012</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Objective. The aim of this study was to compare type I collagen degradation by Candida albicans i...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Objective. The aim of this study was to compare type I collagen degradation by Candida albicans isolated from oral mucosa (M) and cavitated active dentinal caries (CAD) of HIV-infected children. Study Design. To verify the proteolytic activity, the specimens were cultivated in brain-heart infusion medium and the supernatants were incubated in the presence or absence of type I collagen at 37°C for 12 hours and analyzed using 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Intensity of the bands on the gels was assessed by densitometric analysis using a scanner and images analyzed with software from Kodak Digital Science EDAS 120. Results. Supernatants of all the C. albicans degraded type I collagen: that from M, on average, by 38.3% (SD 21.67) and that from CAD by 54% (SD 25.94; Wilcoxon test: P Ͻ .05). Predisposing factors had no association with the percentage of type I collagen degradation (Mann-Whitney test: P Ͼ .05). Conclusions. Candida albicans from different sites of the oral cavity of HIV-infected children has proteolytic activity for type I HIV-infected children are more susceptible to Candida albicans infections. This may be associated with the high prevalence of caries in these patients. Studies that investigate the possible etiologic factors for this high prevalence, such as our research, are very relevant.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="eae03a5f3124b8ef731decce0616d277" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":45612670,"asset_id":13178835,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/45612670/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="13178835"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="13178835"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 13178835; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=13178835]").text(description); $(".js-view-count[data-work-id=13178835]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 13178835; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='13178835']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "eae03a5f3124b8ef731decce0616d277" } } $('.js-work-strip[data-work-id=13178835]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":13178835,"title":"Differential collagenolytic activity of Candida albicans isolated from oral mucosa and dentinal carious lesions of HIV-infected children","translated_title":"","metadata":{"grobid_abstract":"Objective. The aim of this study was to compare type I collagen degradation by Candida albicans isolated from oral mucosa (M) and cavitated active dentinal caries (CAD) of HIV-infected children. Study Design. To verify the proteolytic activity, the specimens were cultivated in brain-heart infusion medium and the supernatants were incubated in the presence or absence of type I collagen at 37°C for 12 hours and analyzed using 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Intensity of the bands on the gels was assessed by densitometric analysis using a scanner and images analyzed with software from Kodak Digital Science EDAS 120. Results. Supernatants of all the C. albicans degraded type I collagen: that from M, on average, by 38.3% (SD 21.67) and that from CAD by 54% (SD 25.94; Wilcoxon test: P Ͻ .05). Predisposing factors had no association with the percentage of type I collagen degradation (Mann-Whitney test: P Ͼ .05). Conclusions. Candida albicans from different sites of the oral cavity of HIV-infected children has proteolytic activity for type I HIV-infected children are more susceptible to Candida albicans infections. This may be associated with the high prevalence of caries in these patients. Studies that investigate the possible etiologic factors for this high prevalence, such as our research, are very relevant.","publication_date":{"day":null,"month":null,"year":2012,"errors":{}},"publication_name":"Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology","grobid_abstract_attachment_id":45612670},"translated_abstract":null,"internal_url":"https://www.academia.edu/13178835/Differential_collagenolytic_activity_of_Candida_albicans_isolated_from_oral_mucosa_and_dentinal_carious_lesions_of_HIV_infected_children","translated_internal_url":"","created_at":"2015-06-22T10:19:15.085-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":32431858,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":1361157,"work_id":13178835,"tagging_user_id":32431858,"tagged_user_id":null,"co_author_invite_id":434361,"email":"m***a@hotmail.com","display_order":null,"name":"Maristela Portela","title":"Differential collagenolytic activity of Candida albicans isolated from oral mucosa and dentinal carious lesions of HIV-infected children"},{"id":1361162,"work_id":13178835,"tagging_user_id":32431858,"tagged_user_id":null,"co_author_invite_id":434363,"email":"r***s@praiadosol.com.br","display_order":null,"name":"Rosangela Maria Araújo Soares","title":"Differential collagenolytic activity of Candida albicans isolated from oral mucosa and dentinal carious lesions of HIV-infected children"},{"id":1361165,"work_id":13178835,"tagging_user_id":32431858,"tagged_user_id":null,"co_author_invite_id":432623,"email":"g***c@biof.ufrj.br","display_order":null,"name":"Gloria Fernanda Barbosa Araújo Castro","title":"Differential collagenolytic activity of Candida albicans isolated from oral mucosa and dentinal carious lesions of HIV-infected children"},{"id":1360972,"work_id":13178835,"tagging_user_id":32431858,"tagged_user_id":26501371,"co_author_invite_id":null,"email":"r***s@micro.ufrj.br","affiliation":"Universidade Federal do Rio de Janeiro (UFRJ)","display_order":null,"name":"Araújo Soares","title":"Differential collagenolytic activity of Candida albicans isolated from oral mucosa and dentinal carious lesions of HIV-infected children"},{"id":1361166,"work_id":13178835,"tagging_user_id":32431858,"tagged_user_id":null,"co_author_invite_id":434364,"email":"c***o@urbi.com.br","display_order":null,"name":"Gloria Castro","title":"Differential collagenolytic activity of Candida albicans isolated from oral mucosa and dentinal carious lesions of HIV-infected children"},{"id":1361001,"work_id":13178835,"tagging_user_id":32431858,"tagged_user_id":32528887,"co_author_invite_id":300859,"email":"r***s@gmail.com","affiliation":"Universidade Federal do Rio de Janeiro (UFRJ)","display_order":null,"name":"R. Soares","title":"Differential collagenolytic activity of Candida albicans isolated from oral mucosa and dentinal carious lesions of HIV-infected children"},{"id":1361164,"work_id":13178835,"tagging_user_id":32431858,"tagged_user_id":null,"co_author_invite_id":203099,"email":"d***s@dpf.gov.br","display_order":null,"name":"Madeleine Chagas","title":"Differential collagenolytic activity of Candida albicans isolated from oral mucosa and dentinal carious lesions of HIV-infected children"}],"downloadable_attachments":[{"id":45612670,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/45612670/thumbnails/1.jpg","file_name":"Differential_collagenolytic_activity_of_20160513-23640-tuzcu9.pdf","download_url":"https://www.academia.edu/attachments/45612670/download_file","bulk_download_file_name":"Differential_collagenolytic_activity_of.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/45612670/Differential_collagenolytic_activity_of_20160513-23640-tuzcu9-libre.pdf?1463201300=\u0026response-content-disposition=attachment%3B+filename%3DDifferential_collagenolytic_activity_of.pdf\u0026Expires=1743616266\u0026Signature=B5S9n8I-7qSDrgPjChKJJ~J0oQWlQhF6bM1ul4SnwAek-dDC-8XTdj34ar7fHFvUYSVNr4Y2AVi7N6QPspIhswWaMsgi9aYJ-0R2DErganuBg8cS1IoY~1T2cMKar4rk3t6ihrN-7FYGLfpyaXRqup89VPF2C9f4pe6AUKnGoKxW064u57gtgEnHLvJEG0PkBh5cXjcHhrXd-CzqpaEo0iDuAjPtWyYvcdMcHxrjyIR9ChmjnvI7tA0OdwEioXjOIQBew0YMjwiGk5zH8-I-7p6FLVzi9pK2gGnIIS2UCH6dQljHFEv4GcrJ4Q8MT1wkmNhd1hy309lGv4sOMKG9xQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Differential_collagenolytic_activity_of_Candida_albicans_isolated_from_oral_mucosa_and_dentinal_carious_lesions_of_HIV_infected_children","translated_slug":"","page_count":6,"language":"en","content_type":"Work","summary":"Objective. The aim of this study was to compare type I collagen degradation by Candida albicans isolated from oral mucosa (M) and cavitated active dentinal caries (CAD) of HIV-infected children. Study Design. To verify the proteolytic activity, the specimens were cultivated in brain-heart infusion medium and the supernatants were incubated in the presence or absence of type I collagen at 37°C for 12 hours and analyzed using 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Intensity of the bands on the gels was assessed by densitometric analysis using a scanner and images analyzed with software from Kodak Digital Science EDAS 120. Results. Supernatants of all the C. albicans degraded type I collagen: that from M, on average, by 38.3% (SD 21.67) and that from CAD by 54% (SD 25.94; Wilcoxon test: P Ͻ .05). Predisposing factors had no association with the percentage of type I collagen degradation (Mann-Whitney test: P Ͼ .05). Conclusions. Candida albicans from different sites of the oral cavity of HIV-infected children has proteolytic activity for type I HIV-infected children are more susceptible to Candida albicans infections. This may be associated with the high prevalence of caries in these patients. Studies that investigate the possible etiologic factors for this high prevalence, such as our research, are very relevant.","owner":{"id":32431858,"first_name":"Thais","middle_initials":null,"last_name":"Souto-padrón","page_name":"ThaisSoutopadrón","domain_name":"ufrj","created_at":"2015-06-22T08:20:34.150-07:00","display_name":"Thais Souto-padrón","url":"https://ufrj.academia.edu/ThaisSoutopadr%C3%B3n"},"attachments":[{"id":45612670,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/45612670/thumbnails/1.jpg","file_name":"Differential_collagenolytic_activity_of_20160513-23640-tuzcu9.pdf","download_url":"https://www.academia.edu/attachments/45612670/download_file","bulk_download_file_name":"Differential_collagenolytic_activity_of.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/45612670/Differential_collagenolytic_activity_of_20160513-23640-tuzcu9-libre.pdf?1463201300=\u0026response-content-disposition=attachment%3B+filename%3DDifferential_collagenolytic_activity_of.pdf\u0026Expires=1743616266\u0026Signature=B5S9n8I-7qSDrgPjChKJJ~J0oQWlQhF6bM1ul4SnwAek-dDC-8XTdj34ar7fHFvUYSVNr4Y2AVi7N6QPspIhswWaMsgi9aYJ-0R2DErganuBg8cS1IoY~1T2cMKar4rk3t6ihrN-7FYGLfpyaXRqup89VPF2C9f4pe6AUKnGoKxW064u57gtgEnHLvJEG0PkBh5cXjcHhrXd-CzqpaEo0iDuAjPtWyYvcdMcHxrjyIR9ChmjnvI7tA0OdwEioXjOIQBew0YMjwiGk5zH8-I-7p6FLVzi9pK2gGnIIS2UCH6dQljHFEv4GcrJ4Q8MT1wkmNhd1hy309lGv4sOMKG9xQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":3243,"name":"Nonparametric Statistics","url":"https://www.academia.edu/Documents/in/Nonparametric_Statistics"},{"id":64933,"name":"Child","url":"https://www.academia.edu/Documents/in/Child"},{"id":105826,"name":"Dental Caries","url":"https://www.academia.edu/Documents/in/Dental_Caries"},{"id":131573,"name":"Candida albicans","url":"https://www.academia.edu/Documents/in/Candida_albicans"},{"id":385863,"name":"Mouth mucosa","url":"https://www.academia.edu/Documents/in/Mouth_mucosa"},{"id":558068,"name":"Dentin","url":"https://www.academia.edu/Documents/in/Dentin"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") if (false) { Aedu.setUpFigureCarousel('profile-work-13178835-figures'); } }); </script> <div class="js-work-strip profile--work_container" data-work-id="13178834"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/13178834/Golgi_UDP_GlcNAc_polypeptide_O_%CE%B1_N_Acetyl_d_glucosaminyltransferase_2_TcOGNT2_regulates_trypomastigote_production_and_function_in_Trypanosoma_cruzi"><img alt="Research paper thumbnail of Golgi UDP-GlcNAc:polypeptide O-α-N-Acetyl-d-glucosaminyltransferase 2 (TcOGNT2) regulates trypomastigote production and function in Trypanosoma cruzi" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title">Golgi UDP-GlcNAc:polypeptide O-α-N-Acetyl-d-glucosaminyltransferase 2 (TcOGNT2) regulates trypomastigote production and function in Trypanosoma cruzi</div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://ufrj.academia.edu/ThaisSoutopadr%C3%B3n">Thais Souto-padrón</a> and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/FabricioMontalv%C3%A3o">Fabricio Montalvão</a></span></div><div class="wp-workCard_item"><span>Eukaryotic cell</span><span>, 2014</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">All life cycle stages of the protozoan parasite Trypanosoma cruzi are enveloped by mucin-like gly...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">All life cycle stages of the protozoan parasite Trypanosoma cruzi are enveloped by mucin-like glycoproteins which, despite major changes in their polypeptide cores, are extensively and similarly O-glycosylated. O-Glycan biosynthesis is initiated by the addition of αGlcNAc to Thr in a reaction catalyzed by Golgi UDP-GlcNAc:polypeptide O-α-N-acetyl-d-glucosaminyltransferases (ppαGlcNAcTs), which are encoded by TcOGNT1 and TcOGNT2. We now directly show that TcOGNT2 is associated with the Golgi apparatus of the epimastigote stage and is markedly downregulated in both differentiated metacyclic trypomastigotes (MCTs) and cell culture-derived trypomastigotes (TCTs). The significance of downregulation was examined by forced continued expression of TcOGNT2, which resulted in a substantial increase of TcOGNT2 protein levels but only modestly increased ppαGlcNAcT activity in extracts and altered cell surface glycosylation in TCTs. Constitutive TcOGNT2 overexpression had no discernible effect o...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="13178834"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="13178834"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 13178834; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=13178834]").text(description); $(".js-view-count[data-work-id=13178834]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 13178834; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='13178834']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=13178834]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":13178834,"title":"Golgi UDP-GlcNAc:polypeptide O-α-N-Acetyl-d-glucosaminyltransferase 2 (TcOGNT2) regulates trypomastigote production and function in Trypanosoma cruzi","translated_title":"","metadata":{"abstract":"All life cycle stages of the protozoan parasite Trypanosoma cruzi are enveloped by mucin-like glycoproteins which, despite major changes in their polypeptide cores, are extensively and similarly O-glycosylated. 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In this study, we evaluated whether pretreatment with SP600125 was able to prevent Orthopoxviruses Vaccinia virus (VACV), Cowpox virus (CPXV) and modified Vaccinia virus Ankara (MVA) replication. We found that incubation with SP600125 not only blocked virus-stimulated JNK phosphorylation, but also, significantly reduced</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="36f8aaef8f3e2a4397ec3bd04a009d03" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":45684233,"asset_id":13178833,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/45684233/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="13178833"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="13178833"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 13178833; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=13178833]").text(description); $(".js-view-count[data-work-id=13178833]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 13178833; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='13178833']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "36f8aaef8f3e2a4397ec3bd04a009d03" } } $('.js-work-strip[data-work-id=13178833]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":13178833,"title":"SP600125 inhibits Orthopoxviruses replication in a JNK1/2 -independent manner: Implication as a potential antipoxviral","translated_title":"","metadata":{"abstract":"The pharmacological inhibitor SP600125 [anthra(1,9-cd)pyrazol-6(2H)-one 1,9-pyrazoloanthrone] has been largely employed as a c-JUN N-terminal kinase (JNK1/2) inhibitor. 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We found that incubation with SP600125 not only blocked virus-stimulated JNK phosphorylation, but also, significantly reduced","owner":{"id":32431858,"first_name":"Thais","middle_initials":null,"last_name":"Souto-padrón","page_name":"ThaisSoutopadrón","domain_name":"ufrj","created_at":"2015-06-22T08:20:34.150-07:00","display_name":"Thais Souto-padrón","url":"https://ufrj.academia.edu/ThaisSoutopadr%C3%B3n"},"attachments":[{"id":45684233,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/45684233/thumbnails/1.jpg","file_name":"j.antiviral.2011.10.02020160516-23030-1cx44so.pdf","download_url":"https://www.academia.edu/attachments/45684233/download_file","bulk_download_file_name":"SP600125_inhibits_Orthopoxviruses_replic.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/45684233/j.antiviral.2011.10.02020160516-23030-1cx44so-libre.pdf?1463449993=\u0026response-content-disposition=attachment%3B+filename%3DSP600125_inhibits_Orthopoxviruses_replic.pdf\u0026Expires=1743616267\u0026Signature=TxlZxP5IKtvFfLUfHMo63kGUzfY6PZlctWgqkmkQedfS6rvqSVbqXxSNwZVgRCbMB-NIvy6daYqIa8mAAexV~xugWZzCBi7sd6ZPdNYB7GQ2AVeytWwg0x6KuEJ8AgKHIYiSNmTxcDtmMpMS0HjHtWE0SsS~YsWzWjPw7as9e3ZZUnc7JWOhZdUaUKGjjcKsmDCXG~ysNfTxugCxSCwY1pDdVkmYLeSwk3pYfiYTEBzfwfdSBe4H9XwwcZrrQryYGkA6PqAYTtBo5hVpEF0AqsybY-9Gk0Wa4IaWMctGSHoYvHzq9dkPQrbT6krtVed0CZjGBXk6Sbinb4HwANjeUQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":6947,"name":"Medical Microbiology","url":"https://www.academia.edu/Documents/in/Medical_Microbiology"},{"id":57570,"name":"Cercopithecus aethiops","url":"https://www.academia.edu/Documents/in/Cercopithecus_aethiops"},{"id":57808,"name":"Cell line","url":"https://www.academia.edu/Documents/in/Cell_line"},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice"},{"id":172083,"name":"Phosphorylation","url":"https://www.academia.edu/Documents/in/Phosphorylation"},{"id":213910,"name":"Mitogen Activated Protein Kinase","url":"https://www.academia.edu/Documents/in/Mitogen_Activated_Protein_Kinase"},{"id":420887,"name":"Viral Infection","url":"https://www.academia.edu/Documents/in/Viral_Infection"},{"id":561002,"name":"Vaccinia Virus","url":"https://www.academia.edu/Documents/in/Vaccinia_Virus"},{"id":584986,"name":"Cowpox virus","url":"https://www.academia.edu/Documents/in/Cowpox_virus"},{"id":792401,"name":"Antiviral","url":"https://www.academia.edu/Documents/in/Antiviral"},{"id":1292080,"name":"Vero cells","url":"https://www.academia.edu/Documents/in/Vero_cells"},{"id":1622318,"name":"Antiviral Agents","url":"https://www.academia.edu/Documents/in/Antiviral_Agents"},{"id":2212891,"name":"Orthopoxvirus","url":"https://www.academia.edu/Documents/in/Orthopoxvirus"}],"urls":[{"id":4894869,"url":"http://www.sciencedirect.com/science/article/pii/S0166354211004839"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") if (false) { Aedu.setUpFigureCarousel('profile-work-13178833-figures'); } }); </script> <div class="js-work-strip profile--work_container" data-work-id="13178832"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/13178832/Trypanosomatids_Odd_Organisms_Devastating_Diseases"><img alt="Research paper thumbnail of Trypanosomatids: Odd Organisms, Devastating Diseases" class="work-thumbnail" src="https://attachments.academia-assets.com/45612681/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/13178832/Trypanosomatids_Odd_Organisms_Devastating_Diseases">Trypanosomatids: Odd Organisms, Devastating Diseases</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://ufrj.academia.edu/ThaisSoutopadr%C3%B3n">Thais Souto-padrón</a> and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/LucianaZimmermann">Luciana Zimmermann</a></span></div><div class="wp-workCard_item"><span>The Open Parasitology Journal</span><span>, 2010</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Trypanosomatids cause many diseases in and on animals (including humans) and plants. Altogether, ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Trypanosomatids cause many diseases in and on animals (including humans) and plants. Altogether, about 37 million people are infected with Trypanosoma brucei (African sleeping sickness), Trypanosoma cruzi (Chagas disease) and Leishmania species (distinct forms of leishmaniasis worldwide). The class Kinetoplastea is divided into the subclasses Prokinetoplastina (order Prokinetoplastida) and Metakinetoplastina (orders Eubodonida, Parabodonida, Neobodonida and Trypanosomatida) [1,2]. The Prokinetoplastida, Eubodonida, Parabodonida and Neobodonida can be free-living, commensalic or parasitic; however, all members of theTrypanosomatida are parasitic. Although they seem like typical protists under the microscope the kinetoplastids have some unique features. In this review we will give an overview of the family Trypanosomatidae, with particular emphasis on some of its "peculiarities" (a single ramified mitochondrion; unusual mitochondrial DNA, the kinetoplast; a complex form of mitochondrial RNA editing; transcription of all protein-encoding genes polycistronically; trans-splicing of all mRNA transcripts; the glycolytic pathway within glycosomes; T. brucei variable surface glycoproteins and T. cruzi ability to escape from the phagocytic vacuoles), as well as the major diseases caused by members of this family. However, the present review does not cover all trypanosomatids; for example, the insect trypanosomatids are underrepresented here. On the other hand, reviews on this particular group of parasites have been written by experts in the field .</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="0fca710fac3e052916c641cbe31b7e75" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":45612681,"asset_id":13178832,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/45612681/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="13178832"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="13178832"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 13178832; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=13178832]").text(description); $(".js-view-count[data-work-id=13178832]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 13178832; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='13178832']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "0fca710fac3e052916c641cbe31b7e75" } } $('.js-work-strip[data-work-id=13178832]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":13178832,"title":"Trypanosomatids: Odd Organisms, Devastating Diseases","translated_title":"","metadata":{"grobid_abstract":"Trypanosomatids cause many diseases in and on animals (including humans) and plants. Altogether, about 37 million people are infected with Trypanosoma brucei (African sleeping sickness), Trypanosoma cruzi (Chagas disease) and Leishmania species (distinct forms of leishmaniasis worldwide). The class Kinetoplastea is divided into the subclasses Prokinetoplastina (order Prokinetoplastida) and Metakinetoplastina (orders Eubodonida, Parabodonida, Neobodonida and Trypanosomatida) [1,2]. The Prokinetoplastida, Eubodonida, Parabodonida and Neobodonida can be free-living, commensalic or parasitic; however, all members of theTrypanosomatida are parasitic. Although they seem like typical protists under the microscope the kinetoplastids have some unique features. In this review we will give an overview of the family Trypanosomatidae, with particular emphasis on some of its \"peculiarities\" (a single ramified mitochondrion; unusual mitochondrial DNA, the kinetoplast; a complex form of mitochondrial RNA editing; transcription of all protein-encoding genes polycistronically; trans-splicing of all mRNA transcripts; the glycolytic pathway within glycosomes; T. brucei variable surface glycoproteins and T. cruzi ability to escape from the phagocytic vacuoles), as well as the major diseases caused by members of this family. However, the present review does not cover all trypanosomatids; for example, the insect trypanosomatids are underrepresented here. 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Altogether, about 37 million people are infected with Trypanosoma brucei (African sleeping sickness), Trypanosoma cruzi (Chagas disease) and Leishmania species (distinct forms of leishmaniasis worldwide). The class Kinetoplastea is divided into the subclasses Prokinetoplastina (order Prokinetoplastida) and Metakinetoplastina (orders Eubodonida, Parabodonida, Neobodonida and Trypanosomatida) [1,2]. The Prokinetoplastida, Eubodonida, Parabodonida and Neobodonida can be free-living, commensalic or parasitic; however, all members of theTrypanosomatida are parasitic. Although they seem like typical protists under the microscope the kinetoplastids have some unique features. In this review we will give an overview of the family Trypanosomatidae, with particular emphasis on some of its \"peculiarities\" (a single ramified mitochondrion; unusual mitochondrial DNA, the kinetoplast; a complex form of mitochondrial RNA editing; transcription of all protein-encoding genes polycistronically; trans-splicing of all mRNA transcripts; the glycolytic pathway within glycosomes; T. brucei variable surface glycoproteins and T. cruzi ability to escape from the phagocytic vacuoles), as well as the major diseases caused by members of this family. However, the present review does not cover all trypanosomatids; for example, the insect trypanosomatids are underrepresented here. On the other hand, reviews on this particular group of parasites have been written by experts in the field .","owner":{"id":32431858,"first_name":"Thais","middle_initials":null,"last_name":"Souto-padrón","page_name":"ThaisSoutopadrón","domain_name":"ufrj","created_at":"2015-06-22T08:20:34.150-07:00","display_name":"Thais Souto-padrón","url":"https://ufrj.academia.edu/ThaisSoutopadr%C3%B3n"},"attachments":[{"id":45612681,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/45612681/thumbnails/1.jpg","file_name":"Trypanosomatids_Odd_Organisms_Devastatin20160513-19714-1b3rymb.pdf","download_url":"https://www.academia.edu/attachments/45612681/download_file","bulk_download_file_name":"Trypanosomatids_Odd_Organisms_Devastatin.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/45612681/Trypanosomatids_Odd_Organisms_Devastatin20160513-19714-1b3rymb-libre.pdf?1463201313=\u0026response-content-disposition=attachment%3B+filename%3DTrypanosomatids_Odd_Organisms_Devastatin.pdf\u0026Expires=1743616267\u0026Signature=efypOeSrLGFoko3StWy5rd6dwZyIkZxt0VJ21u-QR3kISYEiSsvZJ~wd2rD7cxGKW~TSgXCU0I085NaaeYOHrL9AV6AcElNj2CsAZN1oYJ9qi6AL1qcCHzZdmFJGbPU1nd4hYMUjrjKVBP4-PFoZ0EigeMJ0g8FfxnI-XIABNSFpfbkjEGBPZWhNLX~rVnG8QCgHVLNFyeS~vdeMVI33Zhnb5XaUEEprOVX~Z6sqex2PbWtqirIgQS7cmd4J1Eh2sR425OAnfwmR6K5YAfQOpxXkXzMCeUGaFiXB6ZR~-V1C5dFa39SMTv3IWlwku~8zvT2eAKwuz7Ft8Dp6p8HSNQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") if (false) { Aedu.setUpFigureCarousel('profile-work-13178832-figures'); } }); </script> <div class="js-work-strip profile--work_container" data-work-id="13178831"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/13178831/Transovum_Transmission_of_Trypanosomatid_Cysts_in_the_Milkweed_Bug_Oncopeltus_fasciatus"><img alt="Research paper thumbnail of Transovum Transmission of Trypanosomatid Cysts in the Milkweed Bug, Oncopeltus fasciatus" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title">Transovum Transmission of Trypanosomatid Cysts in the Milkweed Bug, Oncopeltus fasciatus</div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://ufrj.academia.edu/ThaisSoutopadr%C3%B3n">Thais Souto-padrón</a> and <a class="" data-click-track="profile-work-strip-authors" href="https://ufrj.academia.edu/LuizRicardoCostaVasconcellos">Luiz Ricardo Costa Vasconcellos</a></span></div><div class="wp-workCard_item"><span>PLoS ONE</span><span>, 2014</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Leptomonas wallacei is a trypanosomatid that develops promastigotes and cystic forms in the gut o...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Leptomonas wallacei is a trypanosomatid that develops promastigotes and cystic forms in the gut of the hemipteran insect Oncopeltus fasciatus. Insect trypanosomatids are thought to be solely transmitted from one host to another through the ingestion of parasite-contaminated feces. However, here we show that L. wallacei cysts present on the eggshells of eggs laid by O. fasciatus can also act as infective forms that are transmitted to the insect offspring. Newly hatched O. faciatus nymphs are parasite-free, but some of them become contaminated with L. wallacei after feeding on eggshell remnants. The present study is the first report of transovum transmission of a trypanosomatid, a process that may have a relevant role in parasite&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;s within-host population dynamics.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="13178831"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="13178831"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 13178831; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=13178831]").text(description); $(".js-view-count[data-work-id=13178831]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 13178831; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='13178831']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=13178831]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":13178831,"title":"Transovum Transmission of Trypanosomatid Cysts in the Milkweed Bug, Oncopeltus fasciatus","translated_title":"","metadata":{"abstract":"Leptomonas wallacei is a trypanosomatid that develops promastigotes and cystic forms in the gut of the hemipteran insect Oncopeltus fasciatus. 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The present study is the first report of transovum transmission of a trypanosomatid, a process that may have a relevant role in parasite\u0026amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;s within-host population dynamics.","publication_date":{"day":null,"month":null,"year":2014,"errors":{}},"publication_name":"PLoS ONE"},"translated_abstract":"Leptomonas wallacei is a trypanosomatid that develops promastigotes and cystic forms in the gut of the hemipteran insect Oncopeltus fasciatus. Insect trypanosomatids are thought to be solely transmitted from one host to another through the ingestion of parasite-contaminated feces. 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The present study is the first report of transovum transmission of a trypanosomatid, a process that may have a relevant role in parasite\u0026amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;s within-host population dynamics.","owner":{"id":32431858,"first_name":"Thais","middle_initials":null,"last_name":"Souto-padrón","page_name":"ThaisSoutopadrón","domain_name":"ufrj","created_at":"2015-06-22T08:20:34.150-07:00","display_name":"Thais Souto-padrón","url":"https://ufrj.academia.edu/ThaisSoutopadr%C3%B3n"},"attachments":[],"research_interests":[{"id":21953,"name":"Heteroptera","url":"https://www.academia.edu/Documents/in/Heteroptera"},{"id":28235,"name":"Multidisciplinary","url":"https://www.academia.edu/Documents/in/Multidisciplinary"},{"id":220780,"name":"PLoS one","url":"https://www.academia.edu/Documents/in/PLoS_one"},{"id":291136,"name":"Intestines","url":"https://www.academia.edu/Documents/in/Intestines"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") if (false) { Aedu.setUpFigureCarousel('profile-work-13178831-figures'); } }); </script> <div class="js-work-strip profile--work_container" data-work-id="13178829"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/13178829/Gene_expression_changes_induced_by_Trypanosoma_cruzi_shed_microvesicles_in_mammalian_host_cells_relevance_of_tRNA_derived_halves"><img alt="Research paper thumbnail of Gene expression changes induced by Trypanosoma cruzi shed microvesicles in mammalian host cells: relevance of tRNA-derived halves" class="work-thumbnail" src="https://attachments.academia-assets.com/45612715/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/13178829/Gene_expression_changes_induced_by_Trypanosoma_cruzi_shed_microvesicles_in_mammalian_host_cells_relevance_of_tRNA_derived_halves">Gene expression changes induced by Trypanosoma cruzi shed microvesicles in mammalian host cells: relevance of tRNA-derived halves</a></div><div class="wp-workCard_item"><span>BioMed research international</span><span>, 2014</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">At present, noncoding small RNAs are recognized as key players in novel forms of posttranscriptio...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">At present, noncoding small RNAs are recognized as key players in novel forms of posttranscriptional gene regulation in most eukaryotes. However, canonical small RNA pathways seem to be lost or excessively simplified in some unicellular organisms including Trypanosoma cruzi which lack functional RNAi pathways. Recently, we reported the presence of alternate small RNA pathways in T. cruzi mainly represented by homogeneous populations of tRNA- and rRNA-derived small RNAs, which are secreted to the extracellular medium included in extracellular vesicles. Extracellular vesicle cargo could be delivered to other parasites and to mammalian susceptible cells promoting metacyclogenesis and conferring susceptibility to infection, respectively. Here we analyzed the changes in gene expression of host HeLa cells induced by extracellular vesicles from T. cruzi. As assessed by microarray assays a large set of genes in HeLa cells were differentially expressed upon incorporation of T. cruzi-derived ...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="f506750d9019e17863078772c27fe400" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":45612715,"asset_id":13178829,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/45612715/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="13178829"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="13178829"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 13178829; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=13178829]").text(description); $(".js-view-count[data-work-id=13178829]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 13178829; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='13178829']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "f506750d9019e17863078772c27fe400" } } $('.js-work-strip[data-work-id=13178829]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":13178829,"title":"Gene expression changes induced by Trypanosoma cruzi shed microvesicles in mammalian host cells: relevance of tRNA-derived halves","translated_title":"","metadata":{"abstract":"At present, noncoding small RNAs are recognized as key players in novel forms of posttranscriptional gene regulation in most eukaryotes. 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