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Bhowmik</title> <meta name="description" content="Search results for: Prodipta Bhowmik"> <meta name="keywords" content="Prodipta Bhowmik"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/css/site.css?v=150220211555" rel="stylesheet"> </head> <body> <header> <div class="container"> <nav class="navbar navbar-expand-lg navbar-light"> <a class="navbar-brand" href="https://waset.org"> <img src="https://cdn.waset.org/static/images/wasetc.png" alt="Open Science Research Excellence" title="Open Science Research Excellence" /> </a> <button class="d-block d-lg-none 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action="https://publications.waset.org/abstracts/search"> <div id="custom-search-input"> <div class="input-group"> <i class="fas fa-search"></i> <input type="text" class="search-query" name="q" placeholder="Author, Title, Abstract, Keywords" value="Prodipta Bhowmik"> <input type="submit" class="btn_search" value="Search"> </div> </div> </form> </div> </div> <div class="row mt-3"> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Commenced</strong> in January 2007</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Frequency:</strong> Monthly</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Edition:</strong> International</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Paper Count:</strong> 15</div> </div> </div> </div> <h1 class="mt-3 mb-3 text-center" style="font-size:1.6rem;">Search results for: Prodipta Bhowmik</h1> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">15</span> Vehicle Speed Estimation Using Image Processing</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Prodipta%20Bhowmik">Prodipta Bhowmik</a>, <a href="https://publications.waset.org/abstracts/search?q=Poulami%20Saha"> Poulami Saha</a>, <a href="https://publications.waset.org/abstracts/search?q=Preety%20Mehra"> Preety Mehra</a>, <a href="https://publications.waset.org/abstracts/search?q=Yogesh%20Soni"> Yogesh Soni</a>, <a href="https://publications.waset.org/abstracts/search?q=Triloki%20Nath%20Jha"> Triloki Nath Jha</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In India, the smart city concept is growing day by day. So, for smart city development, a better traffic management and monitoring system is a very important requirement. Nowadays, road accidents increase due to more vehicles on the road. Reckless driving is mainly responsible for a huge number of accidents. So, an efficient traffic management system is required for all kinds of roads to control the traffic speed. The speed limit varies from road to road basis. Previously, there was a radar system but due to high cost and less precision, the radar system is unable to become favorable in a traffic management system. Traffic management system faces different types of problems every day and it has become a researchable topic on how to solve this problem. This paper proposed a computer vision and machine learning-based automated system for multiple vehicle detection, tracking, and speed estimation of vehicles using image processing. Detection of vehicles and estimating their speed from a real-time video is tough work to do. The objective of this paper is to detect vehicles and estimate their speed as accurately as possible. So for this, a real-time video is first captured, then the frames are extracted from that video, then from that frames, the vehicles are detected, and thereafter, the tracking of vehicles starts, and finally, the speed of the moving vehicles is estimated. The goal of this method is to develop a cost-friendly system that can able to detect multiple types of vehicles at the same time. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=OpenCV" title="OpenCV">OpenCV</a>, <a href="https://publications.waset.org/abstracts/search?q=Haar%20Cascade%20classifier" title=" Haar Cascade classifier"> Haar Cascade classifier</a>, <a href="https://publications.waset.org/abstracts/search?q=DLIB" title=" DLIB"> DLIB</a>, <a href="https://publications.waset.org/abstracts/search?q=YOLOV3" title=" YOLOV3"> YOLOV3</a>, <a href="https://publications.waset.org/abstracts/search?q=centroid%20tracker" title=" centroid tracker"> centroid tracker</a>, <a href="https://publications.waset.org/abstracts/search?q=vehicle%20detection" title=" vehicle detection"> vehicle detection</a>, <a href="https://publications.waset.org/abstracts/search?q=vehicle%20tracking" title=" vehicle tracking"> vehicle tracking</a>, <a href="https://publications.waset.org/abstracts/search?q=vehicle%20speed%20estimation" title=" vehicle speed estimation"> vehicle speed estimation</a>, <a href="https://publications.waset.org/abstracts/search?q=computer%20vision" title=" computer vision"> computer vision</a> </p> <a href="https://publications.waset.org/abstracts/153549/vehicle-speed-estimation-using-image-processing" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/153549.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">84</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">14</span> Study of Microstructure of Weldment Obtained by Submerged Arc Welding (SAW) on IS 2062 Grade B Mild Steel Plate at Zero Degree Celsius</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ajay%20Biswas">Ajay Biswas</a>, <a href="https://publications.waset.org/abstracts/search?q=Swapan%20Bhaumik"> Swapan Bhaumik</a>, <a href="https://publications.waset.org/abstracts/search?q=Abhijit%20Bhowmik"> Abhijit Bhowmik </a> </p> <p class="card-text"><strong>Abstract:</strong></p> Present experiment has been carried out to study the microstructure of weldment obtained by submerged arc welding on mild steel plate at zero degree Celsius. To study this, bead on plate welding is done by submerged arc welding on the sample plate of heavy duty mild steel of designation IS 2062 grade B, fitted on the special fixture ensuring the plate temperature at zero degree Celsius. Sixteen numbers of such samples are welded by varying the most influencing parameters viz. travel speed, voltage, wire feed rate and electrode stick-out at four different levels. Taguchi’s design of experiment is applied by selecting Taguchi's L16 orthogonal array to restrict the number of experimental runs. Cross sectioned samples are polished and etched to view the weldment. Finally, different zone of the weldment is observed by optical microscope. From the type of microstructure of weldment it is concluded that submerged arc welding is feasible at zero degree Celsius on mild steel plate. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Submerged%20Arc%20Welding" title="Submerged Arc Welding">Submerged Arc Welding</a>, <a href="https://publications.waset.org/abstracts/search?q=zero%20degree%20Celsius" title=" zero degree Celsius"> zero degree Celsius</a>, <a href="https://publications.waset.org/abstracts/search?q=Taguchi%E2%80%99s%20design%20of%20experiment" title=" Taguchi’s design of experiment"> Taguchi’s design of experiment</a>, <a href="https://publications.waset.org/abstracts/search?q=microstructure%20of%20weldment" title=" microstructure of weldment"> microstructure of weldment</a> </p> <a href="https://publications.waset.org/abstracts/20873/study-of-microstructure-of-weldment-obtained-by-submerged-arc-welding-saw-on-is-2062-grade-b-mild-steel-plate-at-zero-degree-celsius" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/20873.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">440</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">13</span> Analyses of Natural Convection Heat Transfer from a Heated Cylinder Mounted in Vertical Duct</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=H.%20Bhowmik">H. Bhowmik</a>, <a href="https://publications.waset.org/abstracts/search?q=A.%20Faisal"> A. Faisal</a>, <a href="https://publications.waset.org/abstracts/search?q=Ahmed%20Al%20Yaarubi"> Ahmed Al Yaarubi</a>, <a href="https://publications.waset.org/abstracts/search?q=Nabil%20Al%20Alawi"> Nabil Al Alawi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Experiments are conducted to analyze the steady-state and the power-on transient natural convection heat transfer from a horizontal cylinder mounted in a vertical up flow circular duct. The heat flux ranges from 177 W/m<sup>2</sup> to 2426 W/m<sup>2</sup> and the Rayleigh number ranges from 1×10<sup>4</sup> to 4.35×10<sup>4</sup>. For natural air flow and constant heat flux condition, the effects of heat transfer around the cylinder under steady-state condition are investigated. The steady-state results compare favorably with that of the available data. The effects of transient heat transfer data on different angular position of the thermocouple (0<sup>o</sup>, 90<sup>o</sup>, 180<sup>o</sup>) are also reported. It is observed that the transient heat transfer around the cylinder is strongly affected by the position of thermocouples. In the transient region, the rate of heat transfer obtained at 90<sup>o</sup> and 180<sup>o</sup> are higher than that of stagnation point (0<sup>o</sup>). Finally, the dependence of the average Nusselt number on Rayleigh number for steady and transient natural convection heat transfer are analyzed, and a correlation equation is presented. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Fourier%20number" title="Fourier number">Fourier number</a>, <a href="https://publications.waset.org/abstracts/search?q=Nusselt%20number" title=" Nusselt number"> Nusselt number</a>, <a href="https://publications.waset.org/abstracts/search?q=Rayleigh%20number" title=" Rayleigh number"> Rayleigh number</a>, <a href="https://publications.waset.org/abstracts/search?q=steady%20state" title=" steady state"> steady state</a>, <a href="https://publications.waset.org/abstracts/search?q=transient" title=" transient"> transient</a> </p> <a href="https://publications.waset.org/abstracts/84493/analyses-of-natural-convection-heat-transfer-from-a-heated-cylinder-mounted-in-vertical-duct" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/84493.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">353</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">12</span> Feasibility Study of Submerged Arc Welding (SAW) on Mild Steel Plate IS 2062 Grade B at Zero Degree Celsius</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ajay%20Biswas">Ajay Biswas</a>, <a href="https://publications.waset.org/abstracts/search?q=Abhijit%20Bhowmik"> Abhijit Bhowmik</a>, <a href="https://publications.waset.org/abstracts/search?q=Saurav%20Datta"> Saurav Datta</a>, <a href="https://publications.waset.org/abstracts/search?q=Swapan%20Bhaumik"> Swapan Bhaumik</a> </p> <p class="card-text"><strong>Abstract:</strong></p> A series of experiments has been carried out to study the feasibility of submerged arc welding (SAW) on mild steel plate of designation IS 2062 grade B. Specimen temperature of which is reduced to zero degree Celsius whereas the ambient temperature is about 25-27 degree Celsius. To observe this, bead on plate submerged arc welding is formed on the specimen plate of heavy duty mild steel of designation IS 2062 grade B, fitted on the special fixture ensuring zero degree Celsius temperature to the specimen plate. Sixteen numbers of cold samples is welded by varying the most influencing parameters viz. Voltage, wire feed rate, travel speed and electrode stick-out at four different levels. Another sixteen numbers of specimens are at normal room temperature are welded by applying same combination of parameters. Those sixteen numbers of specimens are selected based on the design of experiment of Taguchi‘s L16 orthogonal array with the intension of reducing the number of experimental runs. Different attributes of bead geometry of the entire sample for both the situations are measured and compared. It is established that submerged arc welding is feasible at zero degree Celsius on mild steel plate of designation IS 2062 grade B and optimization of the process parameters can also be drawn as a clear response of parameters are obtained. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=geometry%20of%20weldment" title="geometry of weldment">geometry of weldment</a>, <a href="https://publications.waset.org/abstracts/search?q=submerged%20arc%20welding" title=" submerged arc welding"> submerged arc welding</a>, <a href="https://publications.waset.org/abstracts/search?q=Taguchi%E2%80%99s%20design%20of%20experiment" title=" Taguchi’s design of experiment"> Taguchi’s design of experiment</a>, <a href="https://publications.waset.org/abstracts/search?q=zero%20degree%20Celsius" title=" zero degree Celsius"> zero degree Celsius</a> </p> <a href="https://publications.waset.org/abstracts/25708/feasibility-study-of-submerged-arc-welding-saw-on-mild-steel-plate-is-2062-grade-b-at-zero-degree-celsius" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/25708.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">433</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">11</span> Study of the Feasibility of Submerged Arc Welding(SAW) on Mild Steel Plate IS 2062 Grade B at Zero Degree Celsius</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ajay%20Biswas">Ajay Biswas</a>, <a href="https://publications.waset.org/abstracts/search?q=Swapan%20Bhaumik"> Swapan Bhaumik</a>, <a href="https://publications.waset.org/abstracts/search?q=Saurav%20Datta"> Saurav Datta</a>, <a href="https://publications.waset.org/abstracts/search?q=Abhijit%20Bhowmik"> Abhijit Bhowmik</a> </p> <p class="card-text"><strong>Abstract:</strong></p> A series of experiments has been carried out to study the feasibility of submerged arc welding (SAW) on mild steel plate of designation IS 2062 grade B. Specimen temperature of which is reduced to zero degree Celsius whereas the ambient temperature is about 25-27 degree Celsius. To observe this, bead on plate submerged arc welding is formed on the specimen plate of heavy duty mild steel of designation IS 2062 grade B, fitted on the special fixture ensuring zero degree Celsius temperature to the specimen plate. Sixteen numbers of cold samples is welded by varying the most influencing parameters viz. voltage, wire feed rate, travel speed, and electrode stick-out at four different levels. Another sixteen numbers of specimens are at normal room temperature are welded by applying same combination of parameters. Those sixteen numbers of specimens are selected based on the design of experiment of Taguchi‘s L16 orthogonal array with the intension of reducing the number of experimental runs. Different attributes of bead geometry of the entire sample for both the situations are measured and compared. It is established that submerged arc welding is feasible at zero degree Celsius on mild steel plate of designation IS 2062 grade B and optimization of the process parameters can also be drawn as a clear response of parameters are obtained. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=submerged%20arc%20welding" title="submerged arc welding">submerged arc welding</a>, <a href="https://publications.waset.org/abstracts/search?q=zero%20degree%20celsius" title=" zero degree celsius"> zero degree celsius</a>, <a href="https://publications.waset.org/abstracts/search?q=Taguchi%E2%80%99s%20design%20of%20experiment" title=" Taguchi’s design of experiment"> Taguchi’s design of experiment</a>, <a href="https://publications.waset.org/abstracts/search?q=geometry%20of%20weldment" title=" geometry of weldment"> geometry of weldment</a> </p> <a href="https://publications.waset.org/abstracts/21988/study-of-the-feasibility-of-submerged-arc-weldingsaw-on-mild-steel-plate-is-2062-grade-b-at-zero-degree-celsius" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/21988.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">449</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">10</span> Aspects and Studies of Fractal Geometry in Automatic Breast Cancer Detection</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Mrinal%20Kanti%20Bhowmik">Mrinal Kanti Bhowmik</a>, <a href="https://publications.waset.org/abstracts/search?q=Kakali%20Das%20Jr."> Kakali Das Jr.</a>, <a href="https://publications.waset.org/abstracts/search?q=Barin%20Kumar%20De"> Barin Kumar De</a>, <a href="https://publications.waset.org/abstracts/search?q=Debotosh%20Bhattacharjee"> Debotosh Bhattacharjee</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Breast cancer is the most common cancer and a leading cause of death for women in the 35 to 55 age group. Early detection of breast cancer can decrease the mortality rate of breast cancer. Mammography is considered as a ‘Gold Standard’ for breast cancer detection and a very popular modality, presently used for breast cancer screening and detection. The screening of digital mammograms often leads to over diagnosis and a consequence to unnecessary traumatic & painful biopsies. For that reason recent studies involving the use of thermal imaging as a screening technique have generated a growing interest especially in cases where the mammography is limited, as in young patients who have dense breast tissue. Tumor is a significant sign of breast cancer in both mammography and thermography. The tumors are complex in structure and they also exhibit a different statistical and textural features compared to the breast background tissue. Fractal geometry is a geometry which is used to describe this type of complex structure as per their main characteristic, where traditional Euclidean geometry fails. Over the last few years, fractal geometrics have been applied mostly in many medical image (1D, 2D, or 3D) analysis applications. In breast cancer detection using digital mammogram images, also it plays a significant role. Fractal is also used in thermography for early detection of the masses using the thermal texture. This paper presents an overview of the recent aspects and initiatives of fractals in breast cancer detection in both mammography and thermography. The scope of fractal geometry in automatic breast cancer detection using digital mammogram and thermogram images are analysed, which forms a foundation for further study on application of fractal geometry in medical imaging for improving the efficiency of automatic detection. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=fractal" title="fractal">fractal</a>, <a href="https://publications.waset.org/abstracts/search?q=tumor" title=" tumor"> tumor</a>, <a href="https://publications.waset.org/abstracts/search?q=thermography" title=" thermography"> thermography</a>, <a href="https://publications.waset.org/abstracts/search?q=mammography" title=" mammography"> mammography</a> </p> <a href="https://publications.waset.org/abstracts/22188/aspects-and-studies-of-fractal-geometry-in-automatic-breast-cancer-detection" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/22188.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">388</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">9</span> A Study of Soft Soil Improvement by Using Lime Grit</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ashim%20Kanti%20Dey">Ashim Kanti Dey</a>, <a href="https://publications.waset.org/abstracts/search?q=Briti%20Sundar%20Bhowmik"> Briti Sundar Bhowmik</a> </p> <p class="card-text"><strong>Abstract:</strong></p> This paper presents an idea to improve the soft soil by using lime grits which are normally produced as waste product in the paper manufacturing industries. This waste material cannot be used as a construction material because of its light weight, uniform size and poor compaction control. With scarcity in land, effective disposal of lime grit is a major concern of all paper manufacturing industries. Considering its non-plasticity and high permeability characteristics the lime grit may suitably be used as a drainage material for speedy consolidation of cohesive soil. It can also be used to improve the bearing capacity of soft clay. An attempt has been made in this paper to show the usefulness of lime grit in improving the bearing capacity of shallow foundation resting on soft clayey soil. A series of undrained unconsolidated cyclic triaxial tests performed at different area ratios and at three different water contents shows that dynamic shear modulus and damping ratio can be substantially improved with lime grit. Improvement is observed to be more in case of higher area ratio and higher water content. Static triaxial tests were also conducted on lime grit reinforced clayey soil after application of 50 load cycles to determine the effect of lime grit columns on cyclically loaded clayey soils. It is observed that the degradation is less for lime grit stabilized soil. A study of model test with different area ratio of lime column installation is also included to see the field behaviour of lime grit reinforced soil. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=lime%20grit%20column" title="lime grit column">lime grit column</a>, <a href="https://publications.waset.org/abstracts/search?q=area%20ratio" title=" area ratio"> area ratio</a>, <a href="https://publications.waset.org/abstracts/search?q=shear%20modulus" title=" shear modulus"> shear modulus</a>, <a href="https://publications.waset.org/abstracts/search?q=damping%20ratio" title=" damping ratio"> damping ratio</a>, <a href="https://publications.waset.org/abstracts/search?q=strength%20ratio" title=" strength ratio"> strength ratio</a>, <a href="https://publications.waset.org/abstracts/search?q=improvement%20factor" title=" improvement factor"> improvement factor</a>, <a href="https://publications.waset.org/abstracts/search?q=degradation%20factor" title=" degradation factor"> degradation factor</a> </p> <a href="https://publications.waset.org/abstracts/10849/a-study-of-soft-soil-improvement-by-using-lime-grit" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/10849.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">503</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">8</span> Experimental Exploration of Recycled Materials for Potential Application in Interior Design</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=E.%20P.%20Bhowmik">E. P. Bhowmik</a>, <a href="https://publications.waset.org/abstracts/search?q=R.%20Singh"> R. Singh</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Certain materials casually thrown away as by-product household waste, such as used tea leaves, used coffee remnants, eggshells, peanut husks, coconut coir, unwanted paper, and pencil shavings- have scope in the hidden properties that they offer as recyclable raw ingredients. This paper aims to explore and experiment with the sustainable potential of such disposed wastes, obtained from domestic and commercial backgrounds, that could otherwise contribute to the field of interior design if mass-collected and repurposed. Research has been conducted on available recorded methods of mass-collection, storage, and processing of such materials by certain brands, designers, and researchers, as well as the various application and angles possible with regards to re-usage. A questionnaire survey was carried out to understand the willingness of the demographics for efforts of the mass collection and their openness to such unconventional materials for interiors. An experiment was also conducted where the selected waste ingredients were used to create small samples that could be used as decorative panels. Comparisons were made for properties like color, smell, texture, relative durability, and weight- and accordingly, applications were suggested. The experiment, therefore, helped to propose to recycle of the common household as a potential surface finish for floors, walls, and ceilings, and even founding material for furniture and decor accessories such as pottery and lamp shades; for non-structural application in both residential and commercial interiors. Common by-product wastes often see their ends at landfills- laymen unaware of their sustainable possibilities dispose of them. However, processing these waste materials and repurposing them by incorporating them into interiors would serve as a sustainable alternative to ethical dilemmas in the construction of interior design/architecture elements. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=interior%20materials" title="interior materials">interior materials</a>, <a href="https://publications.waset.org/abstracts/search?q=mass-collection" title=" mass-collection"> mass-collection</a>, <a href="https://publications.waset.org/abstracts/search?q=sustainable" title=" sustainable"> sustainable</a>, <a href="https://publications.waset.org/abstracts/search?q=waste%20recycle" title=" waste recycle"> waste recycle</a> </p> <a href="https://publications.waset.org/abstracts/155536/experimental-exploration-of-recycled-materials-for-potential-application-in-interior-design" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/155536.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">104</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">7</span> Biophysical Characterization of the Inhibition of cGAS-DNA Sensing by KicGAS, Kaposi's Sarcoma-Associated Herpesvirus Inhibitor of cGAS</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=D.%20Bhowmik">D. Bhowmik</a>, <a href="https://publications.waset.org/abstracts/search?q=Y.%20Tian"> Y. Tian</a>, <a href="https://publications.waset.org/abstracts/search?q=Q.%20Yin"> Q. Yin</a>, <a href="https://publications.waset.org/abstracts/search?q=F.%20Zhu"> F. Zhu</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Cyclic GMP-AMP synthase (cGAS), recognises cytoplasmic double-stranded DNA (dsDNA), indicative of bacterial and viral infections, as well as the leakage of self DNA by cellular dysfunction and stresses, to elicit the host's immune responses. Viruses also have developed numerous strategies to antagonize the cGAS-STING pathway. Kaposi's sarcoma-associated herpesvirus (KSHV) is a human DNA tumor virus that is the causative agent of Kaposi’s sarcoma and several other malignancies. To persist in the host, consequently causing diseases, KSHV must overcome the host innate immune responses, including the cGAS-STING DNA sensing pathway. We already found that ORF52 or KicGAS (KSHV inhibitor of cGAS), an abundant and basic gamma herpesvirus-conserved tegument protein, directly inhibits cGAS enzymatic activity. To better understand the mechanism, we have performed the biochemical and structural characterization of full-length KicGAS and various mutants in regarding binding to DNA. We observed that KicGAS is capable of self-association and identified the critical residues involved in the oligomerization process. We also characterized the DNA-binding of KicGAS and found that KicGAS cooperatively oligomerizes along the length of the double stranded DNA, the highly conserved basic residues at the c-terminal disordered region are crucial for DNA recognition. Deficiency in oligomerization also affects DNA binding. Thus DNA binding by KicGAS sequesters DNA and prevents it from being detected by cGAS, consequently inhibiting cGAS activation. KicGAS homologues also inhibit cGAS efficiently, suggesting inhibition of cGAS is evolutionarily conserved mechanism among gamma herpesvirus. These results highlight the important viral strategy to evade this innate immune sensor. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Kaposi%27s%20sarcoma-associated%20herpesvirus" title="Kaposi's sarcoma-associated herpesvirus">Kaposi's sarcoma-associated herpesvirus</a>, <a href="https://publications.waset.org/abstracts/search?q=KSHV" title=" KSHV"> KSHV</a>, <a href="https://publications.waset.org/abstracts/search?q=cGAS" title=" cGAS"> cGAS</a>, <a href="https://publications.waset.org/abstracts/search?q=DNA%20binding" title=" DNA binding"> DNA binding</a>, <a href="https://publications.waset.org/abstracts/search?q=inhibition" title=" inhibition"> inhibition</a> </p> <a href="https://publications.waset.org/abstracts/116871/biophysical-characterization-of-the-inhibition-of-cgas-dna-sensing-by-kicgas-kaposis-sarcoma-associated-herpesvirus-inhibitor-of-cgas" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/116871.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">128</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">6</span> Enhanced Iron Accumulation in Chickpea Though Expression of Iron-Regulated Transport and Ferritin Genes</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=T.%20M.%20L.%20Hoang">T. M. L. Hoang</a>, <a href="https://publications.waset.org/abstracts/search?q=G.%20Tan"> G. Tan</a>, <a href="https://publications.waset.org/abstracts/search?q=S.%20D.%20Bhowmik"> S. D. Bhowmik</a>, <a href="https://publications.waset.org/abstracts/search?q=B.%20Williams"> B. Williams</a>, <a href="https://publications.waset.org/abstracts/search?q=A.%20Johnson"> A. Johnson</a>, <a href="https://publications.waset.org/abstracts/search?q=M.%20R.%20Karbaschi"> M. R. Karbaschi</a>, <a href="https://publications.waset.org/abstracts/search?q=Y.%20Cheng"> Y. Cheng</a>, <a href="https://publications.waset.org/abstracts/search?q=H.%20Long"> H. Long</a>, <a href="https://publications.waset.org/abstracts/search?q=S.%20G.%20Mundree"> S. G. Mundree</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Iron deficiency is a worldwide problem affecting both developed and developing countries. Currently, two major approaches namely iron supplementation and food fortification have been used to combat this issue. These measures, however, are limited by the economic status of the targeted demographics. Iron biofortification through genetic modification to enhance the inherent iron content and bioavailability of crops has been employed recently. Several important crops such as rice, wheat, and banana were reported successfully improved iron content via this method, but there is no known study in legumes. Chickpea (Cicer arietinum) is an important leguminous crop that is widely consumed, particularly in India where iron deficiency anaemia is prevalent. Chickpea is also an ideal pulse in the formulation of complementary food between pulses and cereals to improve micronutrient contents. This project aims at generating enhanced ion accumulation and bioavailability chickpea through the exogenous expression of genes related to iron transport and iron homeostasis in chickpea plants. Iron-Regulated Transport (IRT) and Ferritin genes in combination were transformed into chickpea half-embryonic axis by agrobacterium–mediated transformation. Transgenic independent event was confirmed by Southern Blot analysis. T3 leaves and seeds of transgenic chickpea were assessed for iron contents using LA-ICP-MS (Laser Ablation – Inductively Coupled Plasma Mass Spectrometry) and ICP-OES (Inductively Coupled Plasma Optical Emission Spectrometry). The correlation between transgene expression levels and iron content in T3 plants and seeds was assessed using qPCR. Results show that iron content in transgenic chickpea expressing the above genes significantly increased compared to that in non-transgenic controls. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=iron%20biofortification" title="iron biofortification">iron biofortification</a>, <a href="https://publications.waset.org/abstracts/search?q=chickpea" title=" chickpea"> chickpea</a>, <a href="https://publications.waset.org/abstracts/search?q=IRT" title=" IRT"> IRT</a>, <a href="https://publications.waset.org/abstracts/search?q=ferritin" title=" ferritin"> ferritin</a>, <a href="https://publications.waset.org/abstracts/search?q=Agrobacterium-mediated%20transformation" title=" Agrobacterium-mediated transformation"> Agrobacterium-mediated transformation</a>, <a href="https://publications.waset.org/abstracts/search?q=LA-ICP-MS" title=" LA-ICP-MS"> LA-ICP-MS</a>, <a href="https://publications.waset.org/abstracts/search?q=ICP-OES" title=" ICP-OES"> ICP-OES</a> </p> <a href="https://publications.waset.org/abstracts/74885/enhanced-iron-accumulation-in-chickpea-though-expression-of-iron-regulated-transport-and-ferritin-genes" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/74885.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">441</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">5</span> Standard Protocol Selection for Acquisition of Breast Thermogram in Perspective of Early Breast Cancer Detection </h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Mrinal%20Kanti%20Bhowmik">Mrinal Kanti Bhowmik</a>, <a href="https://publications.waset.org/abstracts/search?q=Usha%20Rani%20Gogoi%20Jr."> Usha Rani Gogoi Jr.</a>, <a href="https://publications.waset.org/abstracts/search?q=Anjan%20Kumar%20Ghosh"> Anjan Kumar Ghosh</a>, <a href="https://publications.waset.org/abstracts/search?q=Debotosh%20Bhattacharjee"> Debotosh Bhattacharjee</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In the last few decades, breast thermography has achieved an average sensitivity and specificity of 90% for breast tumor detection. Breast thermography is a non-invasive, cost-effective, painless and radiation-free breast imaging modality which makes a significant contribution to the evaluation and diagnosis of patients, suspected of having breast cancer. An abnormal breast thermogram may indicate significant biological risk for the existence or the development of breast tumors. Breast thermography can detect a breast tumor, when the tumor is in its early stage or when the tumor is in a dense breast. The infrared breast thermography is very sensitive to environmental changes for which acquisition of breast thermography should be performed under strictly controlled conditions by undergoing some standard protocols. Several factors like air, temperature, humidity, etc. are there to be considered for characterizing thermal images as an imperative tool for detecting breast cancer. A detailed study of various breast thermogram acquisition protocols adopted by different researchers in their research work is provided here in this paper. After going through a rigorous study of different breast thermogram acquisition protocols, a new standard breast thermography acquisition setup is proposed here in this paper for proper and accurate capturing of the breast thermograms. The proposed breast thermogram acquisition setup is being built in the Radiology Department, Agartala Government Medical College (AGMC), Govt. of Tripura, Tripura, India. The breast thermograms are captured using FLIR T650sc thermal camera with the thermal sensitivity of 20 mK at 30 degree C. The paper is an attempt to highlight the importance of different critical parameters of breast thermography like different thermography views, patient preparation protocols, acquisition room requirements, acquisition system requirements, etc. This paper makes an important contribution by providing a detailed survey and a new efficient approach on breast thermogram capturing. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=acquisition%20protocol" title="acquisition protocol">acquisition protocol</a>, <a href="https://publications.waset.org/abstracts/search?q=breast%20cancer" title=" breast cancer"> breast cancer</a>, <a href="https://publications.waset.org/abstracts/search?q=breast%20thermography" title=" breast thermography"> breast thermography</a>, <a href="https://publications.waset.org/abstracts/search?q=infrared%20thermography" title=" infrared thermography "> infrared thermography </a> </p> <a href="https://publications.waset.org/abstracts/22190/standard-protocol-selection-for-acquisition-of-breast-thermogram-in-perspective-of-early-breast-cancer-detection" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/22190.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">397</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4</span> A Theoretical Study on Pain Assessment through Human Facial Expresion</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Mrinal%20Kanti%20Bhowmik">Mrinal Kanti Bhowmik</a>, <a href="https://publications.waset.org/abstracts/search?q=Debanjana%20Debnath%20Jr."> Debanjana Debnath Jr.</a>, <a href="https://publications.waset.org/abstracts/search?q=Debotosh%20Bhattacharjee"> Debotosh Bhattacharjee</a> </p> <p class="card-text"><strong>Abstract:</strong></p> A facial expression is undeniably the human manners. It is a significant channel for human communication and can be applied to extract emotional features accurately. People in pain often show variations in facial expressions that are readily observable to others. A core of actions is likely to occur or to increase in intensity when people are in pain. To illustrate the changes in the facial appearance, a system known as Facial Action Coding System (FACS) is pioneered by Ekman and Friesen for human observers. According to Prkachin and Solomon, a set of such actions carries the bulk of information about pain. Thus, the Prkachin and Solomon pain intensity (PSPI) metric is defined. So, it is very important to notice that facial expressions, being a behavioral source in communication media, provide an important opening into the issues of non-verbal communication in pain. People express their pain in many ways, and this pain behavior is the basis on which most inferences about pain are drawn in clinical and research settings. Hence, to understand the roles of different pain behaviors, it is essential to study the properties. For the past several years, the studies are concentrated on the properties of one specific form of pain behavior i.e. facial expression. This paper represents a comprehensive study on pain assessment that can model and estimate the intensity of pain that the patient is suffering. It also reviews the historical background of different pain assessment techniques in the context of painful expressions. Different approaches incorporate FACS from psychological views and a pain intensity score using the PSPI metric in pain estimation. This paper investigates in depth analysis of different approaches used in pain estimation and presents different observations found from each technique. It also offers a brief study on different distinguishing features of real and fake pain. Therefore, the necessity of the study lies in the emerging fields of painful face assessment in clinical settings. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=facial%20action%20coding%20system%20%28FACS%29" title="facial action coding system (FACS)">facial action coding system (FACS)</a>, <a href="https://publications.waset.org/abstracts/search?q=pain" title=" pain"> pain</a>, <a href="https://publications.waset.org/abstracts/search?q=pain%20behavior" title=" pain behavior"> pain behavior</a>, <a href="https://publications.waset.org/abstracts/search?q=Prkachin%20and%20Solomon%20pain%20intensity%20%28PSPI%29" title=" Prkachin and Solomon pain intensity (PSPI)"> Prkachin and Solomon pain intensity (PSPI)</a> </p> <a href="https://publications.waset.org/abstracts/22187/a-theoretical-study-on-pain-assessment-through-human-facial-expresion" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/22187.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">346</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3</span> A Survey and Analysis on Inflammatory Pain Detection and Standard Protocol Selection Using Medical Infrared Thermography from Image Processing View Point</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Mrinal%20Kanti%20Bhowmik">Mrinal Kanti Bhowmik</a>, <a href="https://publications.waset.org/abstracts/search?q=Shawli%20Bardhan%20Jr."> Shawli Bardhan Jr.</a>, <a href="https://publications.waset.org/abstracts/search?q=Debotosh%20Bhattacharjee"> Debotosh Bhattacharjee</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Human skin containing temperature value more than absolute zero, discharges infrared radiation related to the frequency of the body temperature. The difference in infrared radiation from the skin surface reflects the abnormality present in human body. Considering the difference, detection and forecasting the temperature variation of the skin surface is the main objective of using Medical Infrared Thermography(MIT) as a diagnostic tool for pain detection. Medical Infrared Thermography(MIT) is a non-invasive imaging technique that records and monitors the temperature flow in the body by receiving the infrared radiated from the skin and represent it through thermogram. The intensity of the thermogram measures the inflammation from the skin surface related to pain in human body. Analysis of thermograms provides automated anomaly detection associated with suspicious pain regions by following several image processing steps. The paper represents a rigorous study based survey related to the processing and analysis of thermograms based on the previous works published in the area of infrared thermal imaging for detecting inflammatory pain diseases like arthritis, spondylosis, shoulder impingement, etc. The study also explores the performance analysis of thermogram processing accompanied by thermogram acquisition protocols, thermography camera specification and the types of pain detected by thermography in summarized tabular format. The tabular format provides a clear structural vision of the past works. The major contribution of the paper introduces a new thermogram acquisition standard associated with inflammatory pain detection in human body to enhance the performance rate. The FLIR T650sc infrared camera with high sensitivity and resolution is adopted to increase the accuracy of thermogram acquisition and analysis. The survey of previous research work highlights that intensity distribution based comparison of comparable and symmetric region of interest and their statistical analysis assigns adequate result in case of identifying and detecting physiological disorder related to inflammatory diseases. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=acquisition%20protocol" title="acquisition protocol">acquisition protocol</a>, <a href="https://publications.waset.org/abstracts/search?q=inflammatory%20pain%20detection" title=" inflammatory pain detection"> inflammatory pain detection</a>, <a href="https://publications.waset.org/abstracts/search?q=medical%20infrared%20thermography%20%28MIT%29" title=" medical infrared thermography (MIT)"> medical infrared thermography (MIT)</a>, <a href="https://publications.waset.org/abstracts/search?q=statistical%20analysis" title=" statistical analysis"> statistical analysis</a> </p> <a href="https://publications.waset.org/abstracts/22181/a-survey-and-analysis-on-inflammatory-pain-detection-and-standard-protocol-selection-using-medical-infrared-thermography-from-image-processing-view-point" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/22181.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">342</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2</span> A Geometric Based Hybrid Approach for Facial Feature Localization</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Priya%20Saha">Priya Saha</a>, <a href="https://publications.waset.org/abstracts/search?q=Sourav%20Dey%20Roy%20Jr."> Sourav Dey Roy Jr.</a>, <a href="https://publications.waset.org/abstracts/search?q=Debotosh%20Bhattacharjee"> Debotosh Bhattacharjee</a>, <a href="https://publications.waset.org/abstracts/search?q=Mita%20Nasipuri"> Mita Nasipuri</a>, <a href="https://publications.waset.org/abstracts/search?q=Barin%20Kumar%20De"> Barin Kumar De</a>, <a href="https://publications.waset.org/abstracts/search?q=Mrinal%20Kanti%20Bhowmik"> Mrinal Kanti Bhowmik</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Biometric face recognition technology (FRT) has gained a lot of attention due to its extensive variety of applications in both security and non-security perspectives. It has come into view to provide a secure solution in identification and verification of person identity. Although other biometric based methods like fingerprint scans, iris scans are available, FRT is verified as an efficient technology for its user-friendliness and contact freeness. Accurate facial feature localization plays an important role for many facial analysis applications including biometrics and emotion recognition. But, there are certain factors, which make facial feature localization a challenging task. On human face, expressions can be seen from the subtle movements of facial muscles and influenced by internal emotional states. These non-rigid facial movements cause noticeable alterations in locations of facial landmarks, their usual shapes, which sometimes create occlusions in facial feature areas making face recognition as a difficult problem. The paper proposes a new hybrid based technique for automatic landmark detection in both neutral and expressive frontal and near frontal face images. The method uses the concept of thresholding, sequential searching and other image processing techniques for locating the landmark points on the face. Also, a Graphical User Interface (GUI) based software is designed that could automatically detect 16 landmark points around eyes, nose and mouth that are mostly affected by the changes in facial muscles. The proposed system has been tested on widely used JAFFE and Cohn Kanade database. Also, the system is tested on DeitY-TU face database which is created in the Biometrics Laboratory of Tripura University under the research project funded by Department of Electronics & Information Technology, Govt. of India. The performance of the proposed method has been done in terms of error measure and accuracy. The method has detection rate of 98.82% on JAFFE database, 91.27% on Cohn Kanade database and 93.05% on DeitY-TU database. Also, we have done comparative study of our proposed method with other techniques developed by other researchers. This paper will put into focus emotion-oriented systems through AU detection in future based on the located features. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=biometrics" title="biometrics">biometrics</a>, <a href="https://publications.waset.org/abstracts/search?q=face%20recognition" title=" face recognition"> face recognition</a>, <a href="https://publications.waset.org/abstracts/search?q=facial%20landmarks" title=" facial landmarks"> facial landmarks</a>, <a href="https://publications.waset.org/abstracts/search?q=image%20processing" title=" image processing"> image processing</a> </p> <a href="https://publications.waset.org/abstracts/22182/a-geometric-based-hybrid-approach-for-facial-feature-localization" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/22182.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">412</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1</span> Immunomodulatory Role of Heat Killed Mycobacterium indicus pranii against Cervical Cancer </h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Priyanka%20Bhowmik">Priyanka Bhowmik</a>, <a href="https://publications.waset.org/abstracts/search?q=Subrata%20Majumdar"> Subrata Majumdar</a>, <a href="https://publications.waset.org/abstracts/search?q=Debprasad%20Chattopadhyay"> Debprasad Chattopadhyay</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Background: Cervical cancer is the third major cause of cancer in women and the second most frequent cause of cancer related deaths causing 300,000 deaths annually worldwide. Evasion of immune response by Human Papilloma Virus (HPV), the key contributing factor behind cancer and pre-cancerous lesions of the uterine cervix, makes immunotherapy a necessity to treat this disease. Objective: A Heat killed fraction of Mycobacterium indicus pranii (MIP), a non-pathogenic Mycobacterium has been shown to exhibit cytotoxic effects on different cancer cells, including human cervical carcinoma cell line HeLa. However, the underlying mechanisms remain unknown. The aim of this study is to decipher the mechanism of MIP induced HeLa cell death. Methods: The cytotoxicity of Mycobacterium indicus pranii against HeLa cells was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Apoptosis was detected by annexin V and Propidium iodide (PI) staining. The assessment of reactive oxygen species (ROS) generation and cell cycle analysis were measured by flow cytometry. The expression of apoptosis associated genes was analyzed by real time PCR. Result: MIP could inhibit the proliferation of HeLa cell in a time and dose dependent manner but caused minor damage to normal cells. The induction of apoptosis was confirmed by the cell surface presentation of phosphatidyl serine, DNA fragmentation, and mitochondrial damage. MIP caused very early (as early as 30 minutes) transcriptional activation of p53, followed by a higher activation (32 fold) at 24 hours suggesting prime importance of p53 in MIP-induced apoptosis in HeLa cell. The up regulation of p53 dependent pro-apoptotic genes Bax, Bak, PUMA, and Noxa followed a lag phase that was required for the transcriptional p53 program. MIP also caused the transcriptional up regulation of Toll like receptor 2 and 4 after 30 minutes of MIP treatment suggesting recognition of MIP by toll like receptors. Moreover, MIP caused the inhibition of expression of HPV anti apoptotic gene E6, which is known to interfere with p53/PUMA/Bax apoptotic cascade. This inhibition might have played a role in transcriptional up regulation of PUMA and subsequently apoptosis. ROS was generated transiently which was concomitant with the highest transcription activation of p53 suggesting a plausible feedback loop network of p53 and ROS in the apoptosis of HeLa cells. Scavenger of ROS, such as N-acetyl-L-cysteine, decreased apoptosis suggesting ROS is an important effector of MIP induced apoptosis. Conclusion: Taken together, MIP possesses full potential to be a novel therapeutic agent in the clinical treatment of cervical cancer. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=cancer" title="cancer">cancer</a>, <a href="https://publications.waset.org/abstracts/search?q=mycobacterium" title=" mycobacterium"> mycobacterium</a>, <a href="https://publications.waset.org/abstracts/search?q=immunity" title=" immunity"> immunity</a>, <a href="https://publications.waset.org/abstracts/search?q=immunotherapy." title=" immunotherapy."> immunotherapy.</a> </p> <a href="https://publications.waset.org/abstracts/80727/immunomodulatory-role-of-heat-killed-mycobacterium-indicus-pranii-against-cervical-cancer" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/80727.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">249</span> </span> </div> </div> </div> </main> <footer> <div id="infolinks" class="pt-3 pb-2"> <div class="container"> <div style="background-color:#f5f5f5;" class="p-3"> <div class="row"> <div class="col-md-2"> <ul class="list-unstyled"> About <li><a href="https://waset.org/page/support">About Us</a></li> <li><a href="https://waset.org/page/support#legal-information">Legal</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/WASET-16th-foundational-anniversary.pdf">WASET celebrates its 16th foundational anniversary</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Account <li><a href="https://waset.org/profile">My Account</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Explore <li><a href="https://waset.org/disciplines">Disciplines</a></li> <li><a href="https://waset.org/conferences">Conferences</a></li> <li><a href="https://waset.org/conference-programs">Conference Program</a></li> <li><a href="https://waset.org/committees">Committees</a></li> <li><a href="https://publications.waset.org">Publications</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Research <li><a href="https://publications.waset.org/abstracts">Abstracts</a></li> <li><a href="https://publications.waset.org">Periodicals</a></li> <li><a href="https://publications.waset.org/archive">Archive</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Open Science <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Open-Science-Philosophy.pdf">Open Science Philosophy</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Open-Science-Award.pdf">Open Science Award</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Open-Society-Open-Science-and-Open-Innovation.pdf">Open Innovation</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Postdoctoral-Fellowship-Award.pdf">Postdoctoral Fellowship Award</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Scholarly-Research-Review.pdf">Scholarly Research Review</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Support <li><a href="https://waset.org/page/support">Support</a></li> <li><a href="https://waset.org/profile/messages/create">Contact Us</a></li> <li><a href="https://waset.org/profile/messages/create">Report Abuse</a></li> </ul> </div> </div> </div> </div> </div> <div class="container text-center"> <hr style="margin-top:0;margin-bottom:.3rem;"> <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank" class="text-muted small">Creative Commons Attribution 4.0 International License</a> <div id="copy" class="mt-2">© 2024 World Academy of Science, Engineering and Technology</div> </div> </footer> <a href="javascript:" id="return-to-top"><i class="fas fa-arrow-up"></i></a> <div class="modal" id="modal-template"> <div class="modal-dialog"> <div class="modal-content"> <div class="row m-0 mt-1"> <div class="col-md-12"> <button type="button" class="close" data-dismiss="modal" aria-label="Close"><span aria-hidden="true">×</span></button> </div> </div> <div class="modal-body"></div> </div> </div> </div> <script src="https://cdn.waset.org/static/plugins/jquery-3.3.1.min.js"></script> <script src="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/js/bootstrap.bundle.min.js"></script> <script src="https://cdn.waset.org/static/js/site.js?v=150220211556"></script> <script> jQuery(document).ready(function() { /*jQuery.get("https://publications.waset.org/xhr/user-menu", function (response) { jQuery('#mainNavMenu').append(response); });*/ jQuery.get({ url: "https://publications.waset.org/xhr/user-menu", cache: false }).then(function(response){ jQuery('#mainNavMenu').append(response); }); }); </script> </body> </html>