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type="text/json">{"id":52873,"name":"Virulence","url":"https://www.academia.edu/Documents/in/Virulence?f_ri=111011","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="57570" href="https://www.academia.edu/Documents/in/Cercopithecus_aethiops">Cercopithecus aethiops</a>, <script data-card-contents-for-ri="57570" type="text/json">{"id":57570,"name":"Cercopithecus aethiops","url":"https://www.academia.edu/Documents/in/Cercopithecus_aethiops?f_ri=111011","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="57808" href="https://www.academia.edu/Documents/in/Cell_line">Cell line</a><script data-card-contents-for-ri="57808" type="text/json">{"id":57808,"name":"Cell line","url":"https://www.academia.edu/Documents/in/Cell_line?f_ri=111011","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=18024936]'), work: {"id":18024936,"title":"Attenuation of a 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href="https://www.academia.edu/32020040/Development_of_an_in_vivo_adeno_associated_virus_mediated_siRNA_approach_to_knockdown_tyrosine_hydroxylase_in_the_lateral_retrochiasmatic_area_of_the_ovine_brain">Development of an in vivo adeno-associated virus-mediated siRNA approach to knockdown tyrosine hydroxylase in the lateral retrochiasmatic area of the ovine brain</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/32020040" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="a35a7979ecbefb455c303d863f6871b3" rel="nofollow" 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Science","url":"https://www.academia.edu/Documents/in/Cognitive_Science?f_ri=111011","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="4559" href="https://www.academia.edu/Documents/in/Reproduction">Reproduction</a>, <script data-card-contents-for-ri="4559" type="text/json">{"id":4559,"name":"Reproduction","url":"https://www.academia.edu/Documents/in/Reproduction?f_ri=111011","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="51121" href="https://www.academia.edu/Documents/in/RNA_interference">RNA interference</a><script data-card-contents-for-ri="51121" type="text/json">{"id":51121,"name":"RNA interference","url":"https://www.academia.edu/Documents/in/RNA_interference?f_ri=111011","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=32020040]'), work: {"id":32020040,"title":"Development of an in vivo adeno-associated virus-mediated siRNA 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type="text/json">{"id":38139,"name":"Protein synthesis","url":"https://www.academia.edu/Documents/in/Protein_synthesis?f_ri=111011","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="38831" href="https://www.academia.edu/Documents/in/Signal_Transduction">Signal Transduction</a>, <script data-card-contents-for-ri="38831" type="text/json">{"id":38831,"name":"Signal Transduction","url":"https://www.academia.edu/Documents/in/Signal_Transduction?f_ri=111011","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="63067" href="https://www.academia.edu/Documents/in/Molecular_and_cellular_biology">Molecular and cellular biology</a><script data-card-contents-for-ri="63067" type="text/json">{"id":63067,"name":"Molecular and cellular biology","url":"https://www.academia.edu/Documents/in/Molecular_and_cellular_biology?f_ri=111011","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: 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u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/15496817/Down_regulation_of_ornithine_decarboxylase_by_an_increased_degradation_of_the_enzyme_during_gastrulation_of_Xenopus_laevis">Down-regulation of ornithine decarboxylase by an increased degradation of the enzyme during gastrulation of Xenopus laevis</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/15496817" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="17ccb154bcde4353544bf7ef66094394" rel="nofollow" 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biology","url":"https://www.academia.edu/Documents/in/Biochemistry_and_cell_biology?f_ri=111011"},{"id":1706341,"name":"Gene Transfer","url":"https://www.academia.edu/Documents/in/Gene_Transfer?f_ri=111011"},{"id":1810445,"name":"Gene expression profiling","url":"https://www.academia.edu/Documents/in/Gene_expression_profiling?f_ri=111011"},{"id":1837977,"name":"Molecular Biology Reports","url":"https://www.academia.edu/Documents/in/Molecular_Biology_Reports?f_ri=111011"},{"id":1954130,"name":"Cell Growth","url":"https://www.academia.edu/Documents/in/Cell_Growth?f_ri=111011"},{"id":2039739,"name":"Down-Regulation","url":"https://www.academia.edu/Documents/in/Down-Regulation?f_ri=111011"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_22023722" data-work_id="22023722" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/22023722/Quantitative_evaluation_of_siRNA_delivery_in_vivo">Quantitative evaluation of siRNA delivery in vivo</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Effective small interfering RNA (siRNA)-mediated therapeutics require the siRNA to be delivered into the cellular RNA-induced silencing complex (RISC). Quantitative information of this essential delivery step is currently inferred from... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_22023722" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Effective small interfering RNA (siRNA)-mediated therapeutics require the siRNA to be delivered into the cellular RNA-induced silencing complex (RISC). Quantitative information of this essential delivery step is currently inferred from the efficacy of gene silencing and siRNA uptake in the tissue. Here we report an approach to directly quantify siRNA in the RISC in rodents and monkey. This is achieved by specific immunoprecipitation of the RISC from tissue lysates and quantification of small RNAs in the immunoprecipitates by stem-loop PCR. The method, expected to be independent of delivery vehicle and target, is label-free, and the throughput is acceptable for preclinical animal studies. We characterized a lipid-formulated siRNA by integrating these approaches and obtained a quantitative perspective on siRNA tissue accumulation, RISC loading, and gene silencing. The described methodologies have utility for the study of silencing mechanism, the development of siRNA therapeutics, and ...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/22023722" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="774d586a1059213c620789b3ca4dbc5c" rel="nofollow" data-download="{"attachment_id":42716389,"asset_id":22023722,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/42716389/download_file?st=MTczMjc0Mjc4MSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="43196545" href="https://independent.academia.edu/NellyKuklin">Nelly Kuklin</a><script data-card-contents-for-user="43196545" type="text/json">{"id":43196545,"first_name":"Nelly","last_name":"Kuklin","domain_name":"independent","page_name":"NellyKuklin","display_name":"Nelly Kuklin","profile_url":"https://independent.academia.edu/NellyKuklin?f_ri=111011","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_22023722 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="22023722"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 22023722, container: ".js-paper-rank-work_22023722", }); 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$(".js-view-count[data-work-id=22023722]").text(description); $(".js-view-count-work_22023722").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_22023722").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="22023722"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">12</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="3701" href="https://www.academia.edu/Documents/in/RNA">RNA</a>, <script data-card-contents-for-ri="3701" type="text/json">{"id":3701,"name":"RNA","url":"https://www.academia.edu/Documents/in/RNA?f_ri=111011","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="32923" href="https://www.academia.edu/Documents/in/Gene_Silencing">Gene Silencing</a>, <script data-card-contents-for-ri="32923" type="text/json">{"id":32923,"name":"Gene Silencing","url":"https://www.academia.edu/Documents/in/Gene_Silencing?f_ri=111011","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="35390" href="https://www.academia.edu/Documents/in/Rodentia">Rodentia</a>, <script data-card-contents-for-ri="35390" type="text/json">{"id":35390,"name":"Rodentia","url":"https://www.academia.edu/Documents/in/Rodentia?f_ri=111011","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="51789" href="https://www.academia.edu/Documents/in/Antibodies">Antibodies</a><script data-card-contents-for-ri="51789" type="text/json">{"id":51789,"name":"Antibodies","url":"https://www.academia.edu/Documents/in/Antibodies?f_ri=111011","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=22023722]'), work: {"id":22023722,"title":"Quantitative evaluation of siRNA delivery in vivo","created_at":"2016-02-15T17:56:04.206-08:00","url":"https://www.academia.edu/22023722/Quantitative_evaluation_of_siRNA_delivery_in_vivo?f_ri=111011","dom_id":"work_22023722","summary":"Effective small interfering RNA (siRNA)-mediated therapeutics require the siRNA to be delivered into the cellular RNA-induced silencing complex (RISC). Quantitative information of this essential delivery step is currently inferred from the efficacy of gene silencing and siRNA uptake in the tissue. Here we report an approach to directly quantify siRNA in the RISC in rodents and monkey. This is achieved by specific immunoprecipitation of the RISC from tissue lysates and quantification of small RNAs in the immunoprecipitates by stem-loop PCR. The method, expected to be independent of delivery vehicle and target, is label-free, and the throughput is acceptable for preclinical animal studies. We characterized a lipid-formulated siRNA by integrating these approaches and obtained a quantitative perspective on siRNA tissue accumulation, RISC loading, and gene silencing. The described methodologies have utility for the study of silencing mechanism, the development of siRNA therapeutics, and ...","downloadable_attachments":[{"id":42716389,"asset_id":22023722,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":43196545,"first_name":"Nelly","last_name":"Kuklin","domain_name":"independent","page_name":"NellyKuklin","display_name":"Nelly Kuklin","profile_url":"https://independent.academia.edu/NellyKuklin?f_ri=111011","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":3701,"name":"RNA","url":"https://www.academia.edu/Documents/in/RNA?f_ri=111011","nofollow":false},{"id":32923,"name":"Gene Silencing","url":"https://www.academia.edu/Documents/in/Gene_Silencing?f_ri=111011","nofollow":false},{"id":35390,"name":"Rodentia","url":"https://www.academia.edu/Documents/in/Rodentia?f_ri=111011","nofollow":false},{"id":51789,"name":"Antibodies","url":"https://www.academia.edu/Documents/in/Antibodies?f_ri=111011","nofollow":false},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice?f_ri=111011"},{"id":111011,"name":"Gene transfer techniques","url":"https://www.academia.edu/Documents/in/Gene_transfer_techniques?f_ri=111011"},{"id":216699,"name":"Immunoprecipitation","url":"https://www.academia.edu/Documents/in/Immunoprecipitation?f_ri=111011"},{"id":375054,"name":"Rats","url":"https://www.academia.edu/Documents/in/Rats?f_ri=111011"},{"id":502720,"name":"Gene Targeting","url":"https://www.academia.edu/Documents/in/Gene_Targeting?f_ri=111011"},{"id":573267,"name":"Macaca Mulatta","url":"https://www.academia.edu/Documents/in/Macaca_Mulatta?f_ri=111011"},{"id":1010725,"name":"Protein Binding","url":"https://www.academia.edu/Documents/in/Protein_Binding?f_ri=111011"},{"id":1681026,"name":"Biochemistry and cell biology","url":"https://www.academia.edu/Documents/in/Biochemistry_and_cell_biology?f_ri=111011"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_20428559" data-work_id="20428559" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/20428559/C_versus_N_terminally_linked_melittin_polyethylenimine_conjugates_the_site_of_linkage_strongly_influences_activity_of_DNA_polyplexes">C- versus N-terminally linked melittin-polyethylenimine conjugates: the site of linkage strongly influences activity of DNA polyplexes</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">One major barrier limiting the transfection efficiency of polyplexes is poor endosomal release, especially when small particles are applied. In an approach to overcome this barrier, covalent attachment of the membrane-active peptide... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_20428559" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">One major barrier limiting the transfection efficiency of polyplexes is poor endosomal release, especially when small particles are applied. In an approach to overcome this barrier, covalent attachment of the membrane-active peptide all-(L)-melittin to polyethylenimine (PEI) polyplexes was found to enhance gene transfer efficiency.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/20428559" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="cbd85b8e3a781240a6d86dbe23955754" rel="nofollow" data-download="{"attachment_id":41960439,"asset_id":20428559,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/41960439/download_file?st=MTczMjc0Mjc4MSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="35429931" href="https://independent.academia.edu/EWagner2">Ernst Wagner</a><script data-card-contents-for-user="35429931" type="text/json">{"id":35429931,"first_name":"Ernst","last_name":"Wagner","domain_name":"independent","page_name":"EWagner2","display_name":"Ernst Wagner","profile_url":"https://independent.academia.edu/EWagner2?f_ri=111011","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_20428559 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="20428559"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 20428559, container: ".js-paper-rank-work_20428559", }); });</script></li><li class="js-percentile-work_20428559 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 20428559; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_20428559"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_20428559 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="20428559"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 20428559; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=20428559]").text(description); $(".js-view-count-work_20428559").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_20428559").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="20428559"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">13</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="48057" href="https://www.academia.edu/Documents/in/DNA">DNA</a>, <script data-card-contents-for-ri="48057" type="text/json">{"id":48057,"name":"DNA","url":"https://www.academia.edu/Documents/in/DNA?f_ri=111011","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="84760" href="https://www.academia.edu/Documents/in/Mice">Mice</a>, <script data-card-contents-for-ri="84760" type="text/json">{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice?f_ri=111011","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="111011" href="https://www.academia.edu/Documents/in/Gene_transfer_techniques">Gene transfer techniques</a>, <script data-card-contents-for-ri="111011" type="text/json">{"id":111011,"name":"Gene transfer techniques","url":"https://www.academia.edu/Documents/in/Gene_transfer_techniques?f_ri=111011","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="147066" href="https://www.academia.edu/Documents/in/Liposomes">Liposomes</a><script data-card-contents-for-ri="147066" type="text/json">{"id":147066,"name":"Liposomes","url":"https://www.academia.edu/Documents/in/Liposomes?f_ri=111011","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=20428559]'), work: {"id":20428559,"title":"C- versus N-terminally linked melittin-polyethylenimine conjugates: the site of linkage strongly influences activity of DNA polyplexes","created_at":"2016-01-21T02:45:31.145-08:00","url":"https://www.academia.edu/20428559/C_versus_N_terminally_linked_melittin_polyethylenimine_conjugates_the_site_of_linkage_strongly_influences_activity_of_DNA_polyplexes?f_ri=111011","dom_id":"work_20428559","summary":"One major barrier limiting the transfection efficiency of polyplexes is poor endosomal release, especially when small particles are applied. In an approach to overcome this barrier, covalent attachment of the membrane-active peptide all-(L)-melittin to polyethylenimine (PEI) polyplexes was found to enhance gene transfer efficiency.","downloadable_attachments":[{"id":41960439,"asset_id":20428559,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":35429931,"first_name":"Ernst","last_name":"Wagner","domain_name":"independent","page_name":"EWagner2","display_name":"Ernst Wagner","profile_url":"https://independent.academia.edu/EWagner2?f_ri=111011","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":48057,"name":"DNA","url":"https://www.academia.edu/Documents/in/DNA?f_ri=111011","nofollow":false},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice?f_ri=111011","nofollow":false},{"id":111011,"name":"Gene transfer techniques","url":"https://www.academia.edu/Documents/in/Gene_transfer_techniques?f_ri=111011","nofollow":false},{"id":147066,"name":"Liposomes","url":"https://www.academia.edu/Documents/in/Liposomes?f_ri=111011","nofollow":false},{"id":190363,"name":"Plasmids","url":"https://www.academia.edu/Documents/in/Plasmids?f_ri=111011"},{"id":244814,"name":"Clinical Sciences","url":"https://www.academia.edu/Documents/in/Clinical_Sciences?f_ri=111011"},{"id":419370,"name":"Swine","url":"https://www.academia.edu/Documents/in/Swine?f_ri=111011"},{"id":1031068,"name":"Drug Carriers","url":"https://www.academia.edu/Documents/in/Drug_Carriers?f_ri=111011"},{"id":1137254,"name":"Hydrogen-Ion Concentration","url":"https://www.academia.edu/Documents/in/Hydrogen-Ion_Concentration?f_ri=111011"},{"id":1157148,"name":"Cell Survival","url":"https://www.academia.edu/Documents/in/Cell_Survival?f_ri=111011"},{"id":1254280,"name":"Endosomes","url":"https://www.academia.edu/Documents/in/Endosomes?f_ri=111011"},{"id":1266470,"name":"Polyethylenimine","url":"https://www.academia.edu/Documents/in/Polyethylenimine?f_ri=111011"},{"id":1438957,"name":"Polyethyleneimine","url":"https://www.academia.edu/Documents/in/Polyethyleneimine?f_ri=111011"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_76043038" data-work_id="76043038" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/76043038/Alteration_in_the_IL_2_signal_peptide_affects_secretion_of_proteinsin_vitro_andin_vivo">Alteration in the IL-2 signal peptide affects secretion of proteinsin vitro andin vivo</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/76043038" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="56b058d20837c813f81643ead2e6adca" rel="nofollow" data-download="{"attachment_id":83728256,"asset_id":76043038,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/83728256/download_file?st=MTczMjc0Mjc4MSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="63374588" href="https://independent.academia.edu/ArchibaldMixson">Archibald Mixson</a><script data-card-contents-for-user="63374588" type="text/json">{"id":63374588,"first_name":"Archibald","last_name":"Mixson","domain_name":"independent","page_name":"ArchibaldMixson","display_name":"Archibald Mixson","profile_url":"https://independent.academia.edu/ArchibaldMixson?f_ri=111011","photo":"https://0.academia-photos.com/63374588/59794695/48045265/s65_archibald.mixson.png"}</script></span></span></li><li class="js-paper-rank-work_76043038 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="76043038"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 76043038, container: ".js-paper-rank-work_76043038", }); });</script></li><li class="js-percentile-work_76043038 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 76043038; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_76043038"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_76043038 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="76043038"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 76043038; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=76043038]").text(description); $(".js-view-count-work_76043038").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_76043038").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="76043038"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">12</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="57808" href="https://www.academia.edu/Documents/in/Cell_line">Cell line</a>, <script data-card-contents-for-ri="57808" type="text/json">{"id":57808,"name":"Cell line","url":"https://www.academia.edu/Documents/in/Cell_line?f_ri=111011","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="84760" href="https://www.academia.edu/Documents/in/Mice">Mice</a>, <script data-card-contents-for-ri="84760" type="text/json">{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice?f_ri=111011","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="111011" href="https://www.academia.edu/Documents/in/Gene_transfer_techniques">Gene transfer techniques</a>, <script data-card-contents-for-ri="111011" type="text/json">{"id":111011,"name":"Gene transfer techniques","url":"https://www.academia.edu/Documents/in/Gene_transfer_techniques?f_ri=111011","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="204435" href="https://www.academia.edu/Documents/in/Alkaline_phosphatase">Alkaline phosphatase</a><script data-card-contents-for-ri="204435" type="text/json">{"id":204435,"name":"Alkaline phosphatase","url":"https://www.academia.edu/Documents/in/Alkaline_phosphatase?f_ri=111011","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=76043038]'), work: {"id":76043038,"title":"Alteration in the IL-2 signal peptide affects secretion of proteinsin vitro andin vivo","created_at":"2022-04-10T17:08:08.482-07:00","url":"https://www.academia.edu/76043038/Alteration_in_the_IL_2_signal_peptide_affects_secretion_of_proteinsin_vitro_andin_vivo?f_ri=111011","dom_id":"work_76043038","summary":null,"downloadable_attachments":[{"id":83728256,"asset_id":76043038,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":63374588,"first_name":"Archibald","last_name":"Mixson","domain_name":"independent","page_name":"ArchibaldMixson","display_name":"Archibald Mixson","profile_url":"https://independent.academia.edu/ArchibaldMixson?f_ri=111011","photo":"https://0.academia-photos.com/63374588/59794695/48045265/s65_archibald.mixson.png"}],"research_interests":[{"id":57808,"name":"Cell line","url":"https://www.academia.edu/Documents/in/Cell_line?f_ri=111011","nofollow":false},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice?f_ri=111011","nofollow":false},{"id":111011,"name":"Gene transfer techniques","url":"https://www.academia.edu/Documents/in/Gene_transfer_techniques?f_ri=111011","nofollow":false},{"id":204435,"name":"Alkaline phosphatase","url":"https://www.academia.edu/Documents/in/Alkaline_phosphatase?f_ri=111011","nofollow":false},{"id":244814,"name":"Clinical Sciences","url":"https://www.academia.edu/Documents/in/Clinical_Sciences?f_ri=111011"},{"id":437728,"name":"Isoenzymes","url":"https://www.academia.edu/Documents/in/Isoenzymes?f_ri=111011"},{"id":687738,"name":"SIGNAL PEPTIDE","url":"https://www.academia.edu/Documents/in/SIGNAL_PEPTIDE?f_ri=111011"},{"id":809881,"name":"Amino Acid Sequence","url":"https://www.academia.edu/Documents/in/Amino_Acid_Sequence?f_ri=111011"},{"id":809882,"name":"Base Sequence","url":"https://www.academia.edu/Documents/in/Base_Sequence?f_ri=111011"},{"id":1924712,"name":"Interleukin","url":"https://www.academia.edu/Documents/in/Interleukin?f_ri=111011"},{"id":2467566,"name":"Molecular Sequence Data","url":"https://www.academia.edu/Documents/in/Molecular_Sequence_Data?f_ri=111011"},{"id":4079130,"name":"Endostatin","url":"https://www.academia.edu/Documents/in/Endostatin?f_ri=111011"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_72719422" data-work_id="72719422" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/72719422/Gene_transfer_of_Smad7_modulates_injury_induced_conjunctival_wound_healing_in_mice">Gene transfer of Smad7 modulates injury-induced conjunctival wound healing in mice</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">PURPOSE Smad7 is a molecule that blocks the Smad2/3 signal. Herein, we examined the effects of Smad7 gene introduction on post-injury conjunctival wound healing in mice. Its effects on the cultured human subconjunctival fibroblasts (SCFs)... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_72719422" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">PURPOSE Smad7 is a molecule that blocks the Smad2/3 signal. Herein, we examined the effects of Smad7 gene introduction on post-injury conjunctival wound healing in mice. Its effects on the cultured human subconjunctival fibroblasts (SCFs) were also investigated. METHODS A circumferential incision was made in the equatorial conjunctiva by using scissors in the right eye of fully anesthetized adult C57BL/6 mice (n=72). Smad7 cDNA-expressing adenoviral vector was topically applied. The control eye received nonfunctioning adenoviral vector. After 2, 5, 7, and 30 days the eyes were processed for histological or immunohistochemical examination to evaluate wound healing of conjunctiva. The expressions of type-I collagen and growth factors were evaluated by real time-reverse transcriptase-polymerase chain reaction. The effects of Smad7 gene introduction on the cultured human SCFs were also studied. RESULTS Marked Smad7 protein expression was detected in the vector-treated conjunctival epith...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/72719422" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="29d6e68d3810365cfc0ab675f10f84cf" rel="nofollow" data-download="{"attachment_id":81535344,"asset_id":72719422,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/81535344/download_file?st=MTczMjc0Mjc4MSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="39794701" href="https://independent.academia.edu/OsamuYamanaka">Osamu Yamanaka</a><script data-card-contents-for-user="39794701" type="text/json">{"id":39794701,"first_name":"Osamu","last_name":"Yamanaka","domain_name":"independent","page_name":"OsamuYamanaka","display_name":"Osamu Yamanaka","profile_url":"https://independent.academia.edu/OsamuYamanaka?f_ri=111011","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_72719422 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="72719422"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 72719422, container: ".js-paper-rank-work_72719422", }); 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$(".js-view-count[data-work-id=72719422]").text(description); $(".js-view-count-work_72719422").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_72719422").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="72719422"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">9</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="8991" href="https://www.academia.edu/Documents/in/Wound_Healing">Wound Healing</a>, <script data-card-contents-for-ri="8991" type="text/json">{"id":8991,"name":"Wound Healing","url":"https://www.academia.edu/Documents/in/Wound_Healing?f_ri=111011","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="12071" href="https://www.academia.edu/Documents/in/Immunohistochemistry">Immunohistochemistry</a>, <script data-card-contents-for-ri="12071" type="text/json">{"id":12071,"name":"Immunohistochemistry","url":"https://www.academia.edu/Documents/in/Immunohistochemistry?f_ri=111011","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="28404" href="https://www.academia.edu/Documents/in/Conjunctiva">Conjunctiva</a>, <script data-card-contents-for-ri="28404" type="text/json">{"id":28404,"name":"Conjunctiva","url":"https://www.academia.edu/Documents/in/Conjunctiva?f_ri=111011","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="65171" href="https://www.academia.edu/Documents/in/Smooth_muscle">Smooth muscle</a><script data-card-contents-for-ri="65171" type="text/json">{"id":65171,"name":"Smooth muscle","url":"https://www.academia.edu/Documents/in/Smooth_muscle?f_ri=111011","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=72719422]'), work: {"id":72719422,"title":"Gene transfer of Smad7 modulates injury-induced conjunctival wound healing in mice","created_at":"2022-03-01T14:44:40.995-08:00","url":"https://www.academia.edu/72719422/Gene_transfer_of_Smad7_modulates_injury_induced_conjunctival_wound_healing_in_mice?f_ri=111011","dom_id":"work_72719422","summary":"PURPOSE Smad7 is a molecule that blocks the Smad2/3 signal. Herein, we examined the effects of Smad7 gene introduction on post-injury conjunctival wound healing in mice. Its effects on the cultured human subconjunctival fibroblasts (SCFs) were also investigated. METHODS A circumferential incision was made in the equatorial conjunctiva by using scissors in the right eye of fully anesthetized adult C57BL/6 mice (n=72). Smad7 cDNA-expressing adenoviral vector was topically applied. The control eye received nonfunctioning adenoviral vector. After 2, 5, 7, and 30 days the eyes were processed for histological or immunohistochemical examination to evaluate wound healing of conjunctiva. The expressions of type-I collagen and growth factors were evaluated by real time-reverse transcriptase-polymerase chain reaction. The effects of Smad7 gene introduction on the cultured human SCFs were also studied. RESULTS Marked Smad7 protein expression was detected in the vector-treated conjunctival epith...","downloadable_attachments":[{"id":81535344,"asset_id":72719422,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":39794701,"first_name":"Osamu","last_name":"Yamanaka","domain_name":"independent","page_name":"OsamuYamanaka","display_name":"Osamu Yamanaka","profile_url":"https://independent.academia.edu/OsamuYamanaka?f_ri=111011","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":8991,"name":"Wound Healing","url":"https://www.academia.edu/Documents/in/Wound_Healing?f_ri=111011","nofollow":false},{"id":12071,"name":"Immunohistochemistry","url":"https://www.academia.edu/Documents/in/Immunohistochemistry?f_ri=111011","nofollow":false},{"id":28404,"name":"Conjunctiva","url":"https://www.academia.edu/Documents/in/Conjunctiva?f_ri=111011","nofollow":false},{"id":65171,"name":"Smooth 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u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/48820000/Biolistic_Mediated_Transformation_Protocols_for_Maize_and_Pearl_Millet_Using_Pre_Cultured_Immature_Zygotic_Embryos_and_Embryogenic_Tissue">Biolistic-Mediated Transformation Protocols for Maize and Pearl Millet Using Pre-Cultured Immature Zygotic Embryos and Embryogenic Tissue</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/48820000" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="033c2fe0774a6a053dd81a10fd8917cf" rel="nofollow" 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Media","url":"https://www.academia.edu/Documents/in/Culture_Media?f_ri=111011"},{"id":1681026,"name":"Biochemistry and cell biology","url":"https://www.academia.edu/Documents/in/Biochemistry_and_cell_biology?f_ri=111011"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_35468655" data-work_id="35468655" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/35468655/Zinc_finger_nucleases_looking_toward_translation">Zinc finger nucleases: looking toward translation</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" 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class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/10518851/Engineering_metabolic_pathways_in_plants_by_multigene_transformation">Engineering metabolic pathways in plants by multigene transformation</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/10518851" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="a619264e24cacd73344e743ea13bc87e" rel="nofollow" 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Assay","url":"https://www.academia.edu/Documents/in/Enzyme_Linked_Immunosorbent_Assay?f_ri=111011"},{"id":1640340,"name":"Lycopersicon esculentum","url":"https://www.academia.edu/Documents/in/Lycopersicon_esculentum?f_ri=111011"},{"id":1732722,"name":"Transgenic rice","url":"https://www.academia.edu/Documents/in/Transgenic_rice?f_ri=111011"},{"id":1905343,"name":"Plant Leaves","url":"https://www.academia.edu/Documents/in/Plant_Leaves?f_ri=111011"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_16159939" data-work_id="16159939" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/16159939/Correction_of_endothelial_dysfunction_after_selective_homocysteine_lowering_gene_therapy_reduces_arterial_thrombogenicity_but_has_no_effect_on_atherogenesis">Correction of endothelial dysfunction after selective homocysteine lowering gene therapy reduces arterial thrombogenicity but has no effect on atherogenesis</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/16159939" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="a1a632450fa90210fb6bb3497ba33943" rel="nofollow" data-download="{"attachment_id":42662425,"asset_id":16159939,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/42662425/download_file?st=MTczMjc0Mjc4MSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="35274705" href="https://independent.academia.edu/MarcHoylaerts">Marc Hoylaerts</a><script data-card-contents-for-user="35274705" type="text/json">{"id":35274705,"first_name":"Marc","last_name":"Hoylaerts","domain_name":"independent","page_name":"MarcHoylaerts","display_name":"Marc Hoylaerts","profile_url":"https://independent.academia.edu/MarcHoylaerts?f_ri=111011","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_16159939 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="16159939"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 16159939, container: ".js-paper-rank-work_16159939", }); });</script></li><li class="js-percentile-work_16159939 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 16159939; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_16159939"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_16159939 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="16159939"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 16159939; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=16159939]").text(description); $(".js-view-count-work_16159939").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_16159939").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="16159939"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">21</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="5072" href="https://www.academia.edu/Documents/in/Molecular_Medicine">Molecular Medicine</a>, <script data-card-contents-for-ri="5072" type="text/json">{"id":5072,"name":"Molecular Medicine","url":"https://www.academia.edu/Documents/in/Molecular_Medicine?f_ri=111011","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="7864" href="https://www.academia.edu/Documents/in/Gene_Therapy">Gene Therapy</a>, <script data-card-contents-for-ri="7864" type="text/json">{"id":7864,"name":"Gene Therapy","url":"https://www.academia.edu/Documents/in/Gene_Therapy?f_ri=111011","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="8015" href="https://www.academia.edu/Documents/in/Atherosclerosis">Atherosclerosis</a>, <script data-card-contents-for-ri="8015" type="text/json">{"id":8015,"name":"Atherosclerosis","url":"https://www.academia.edu/Documents/in/Atherosclerosis?f_ri=111011","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="24735" href="https://www.academia.edu/Documents/in/Cardiovascular_disease">Cardiovascular disease</a><script data-card-contents-for-ri="24735" type="text/json">{"id":24735,"name":"Cardiovascular disease","url":"https://www.academia.edu/Documents/in/Cardiovascular_disease?f_ri=111011","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=16159939]'), work: {"id":16159939,"title":"Correction of endothelial dysfunction after selective homocysteine lowering gene therapy reduces arterial thrombogenicity but has no effect on atherogenesis","created_at":"2015-09-25T06:11:19.174-07:00","url":"https://www.academia.edu/16159939/Correction_of_endothelial_dysfunction_after_selective_homocysteine_lowering_gene_therapy_reduces_arterial_thrombogenicity_but_has_no_effect_on_atherogenesis?f_ri=111011","dom_id":"work_16159939","summary":null,"downloadable_attachments":[{"id":42662425,"asset_id":16159939,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":35274705,"first_name":"Marc","last_name":"Hoylaerts","domain_name":"independent","page_name":"MarcHoylaerts","display_name":"Marc Hoylaerts","profile_url":"https://independent.academia.edu/MarcHoylaerts?f_ri=111011","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":5072,"name":"Molecular Medicine","url":"https://www.academia.edu/Documents/in/Molecular_Medicine?f_ri=111011","nofollow":false},{"id":7864,"name":"Gene Therapy","url":"https://www.academia.edu/Documents/in/Gene_Therapy?f_ri=111011","nofollow":false},{"id":8015,"name":"Atherosclerosis","url":"https://www.academia.edu/Documents/in/Atherosclerosis?f_ri=111011","nofollow":false},{"id":24735,"name":"Cardiovascular disease","url":"https://www.academia.edu/Documents/in/Cardiovascular_disease?f_ri=111011","nofollow":false},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice?f_ri=111011"},{"id":111011,"name":"Gene transfer techniques","url":"https://www.academia.edu/Documents/in/Gene_transfer_techniques?f_ri=111011"},{"id":138877,"name":"Vascular endothelium","url":"https://www.academia.edu/Documents/in/Vascular_endothelium?f_ri=111011"},{"id":159958,"name":"Acetylcholine","url":"https://www.academia.edu/Documents/in/Acetylcholine?f_ri=111011"},{"id":192721,"name":"Risk factors","url":"https://www.academia.edu/Documents/in/Risk_factors?f_ri=111011"},{"id":195983,"name":"Homocysteine","url":"https://www.academia.edu/Documents/in/Homocysteine?f_ri=111011"},{"id":197120,"name":"Endothelial dysfunction","url":"https://www.academia.edu/Documents/in/Endothelial_dysfunction?f_ri=111011"},{"id":227285,"name":"Lipoprotein(a)","url":"https://www.academia.edu/Documents/in/Lipoprotein_a_-1?f_ri=111011"},{"id":301381,"name":"Endothelial Function","url":"https://www.academia.edu/Documents/in/Endothelial_Function?f_ri=111011"},{"id":413195,"name":"Time Factors","url":"https://www.academia.edu/Documents/in/Time_Factors?f_ri=111011"},{"id":496063,"name":"Thrombosis","url":"https://www.academia.edu/Documents/in/Thrombosis?f_ri=111011"},{"id":620049,"name":"Risk Factors","url":"https://www.academia.edu/Documents/in/Risk_Factors-1?f_ri=111011"},{"id":969448,"name":"Carotid Artery","url":"https://www.academia.edu/Documents/in/Carotid_Artery?f_ri=111011"},{"id":1035420,"name":"Vasodilation","url":"https://www.academia.edu/Documents/in/Vasodilation?f_ri=111011"},{"id":1192830,"name":"Cystathionine Beta-Synthase","url":"https://www.academia.edu/Documents/in/Cystathionine_Beta-Synthase?f_ri=111011"},{"id":1577521,"name":"Lateral Gene Transfer","url":"https://www.academia.edu/Documents/in/Lateral_Gene_Transfer?f_ri=111011"},{"id":1706341,"name":"Gene Transfer","url":"https://www.academia.edu/Documents/in/Gene_Transfer?f_ri=111011"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_19104865" data-work_id="19104865" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/19104865/Immortalization_of_epithelial_cells">Immortalization of epithelial cells</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/19104865" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="177bc0f5a04be7ffb1e6805a2e75d649" rel="nofollow" data-download="{"attachment_id":40434218,"asset_id":19104865,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/40434218/download_file?st=MTczMjc0Mjc4MSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="39287431" href="https://ucsf.academia.edu/DGruenert">Dieter C Gruenert</a><script data-card-contents-for-user="39287431" type="text/json">{"id":39287431,"first_name":"Dieter","last_name":"Gruenert","domain_name":"ucsf","page_name":"DGruenert","display_name":"Dieter C Gruenert","profile_url":"https://ucsf.academia.edu/DGruenert?f_ri=111011","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_19104865 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="19104865"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 19104865, container: ".js-paper-rank-work_19104865", }); });</script></li><li class="js-percentile-work_19104865 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 19104865; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_19104865"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_19104865 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="19104865"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 19104865; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=19104865]").text(description); $(".js-view-count-work_19104865").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_19104865").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="19104865"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">7</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="84760" href="https://www.academia.edu/Documents/in/Mice">Mice</a>, <script data-card-contents-for-ri="84760" type="text/json">{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice?f_ri=111011","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="111011" href="https://www.academia.edu/Documents/in/Gene_transfer_techniques">Gene transfer techniques</a>, <script data-card-contents-for-ri="111011" type="text/json">{"id":111011,"name":"Gene transfer techniques","url":"https://www.academia.edu/Documents/in/Gene_transfer_techniques?f_ri=111011","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="115707" href="https://www.academia.edu/Documents/in/Viruses">Viruses</a>, <script data-card-contents-for-ri="115707" type="text/json">{"id":115707,"name":"Viruses","url":"https://www.academia.edu/Documents/in/Viruses?f_ri=111011","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="213897" href="https://www.academia.edu/Documents/in/Phenotype">Phenotype</a><script data-card-contents-for-ri="213897" type="text/json">{"id":213897,"name":"Phenotype","url":"https://www.academia.edu/Documents/in/Phenotype?f_ri=111011","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=19104865]'), work: {"id":19104865,"title":"Immortalization of epithelial 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techniques","url":"https://www.academia.edu/Documents/in/Gene_transfer_techniques?f_ri=111011","nofollow":false},{"id":115707,"name":"Viruses","url":"https://www.academia.edu/Documents/in/Viruses?f_ri=111011","nofollow":false},{"id":213897,"name":"Phenotype","url":"https://www.academia.edu/Documents/in/Phenotype?f_ri=111011","nofollow":false},{"id":379416,"name":"Epithelial cells","url":"https://www.academia.edu/Documents/in/Epithelial_cells?f_ri=111011"},{"id":620070,"name":"Transfection","url":"https://www.academia.edu/Documents/in/Transfection?f_ri=111011"},{"id":1915496,"name":"Cytological Techniques","url":"https://www.academia.edu/Documents/in/Cytological_Techniques?f_ri=111011"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_14236010" data-work_id="14236010" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/14236010/Enhancement_of_2_4_dichlorophenoxyacetic_acid_2_4_D_degradation_in_soil_by_dissemination_of_catabolic_plasmids">Enhancement of 2,4-dichlorophenoxyacetic acid (2,4-D) degradation in soil by dissemination of catabolic plasmids</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Few studies have been done to evaluate the transfer of catabolic plasmids from an introduced donor strain to indigenous microbial populations as a means to remediate contaminated soils. In this work we determined the effect of the... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_14236010" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Few studies have been done to evaluate the transfer of catabolic plasmids from an introduced donor strain to indigenous microbial populations as a means to remediate contaminated soils. In this work we determined the effect of the conjugative transfer of two 2,4-D degradative plasmids to indigenous soil bacterial populations on the rate of 2,4-D degradation in soil. We also assessed the influence of the presence of 2,4-D on the number of transconjugants formed. The two plasmids used, pEMT1k and pEMT3k, encode 2,4-D degradative genes (tfd) that differ in DNA sequence as well as gene organisation, and confer different growth rates to Ralstonia eutropha JMP228 when grown with 2,4-D as a sole carbon source. In an agricultural soil (Ardoyen) treated with 2,4-D (100 ppm) there were ca. 10(7) CFU of transconjugants per gram bearing pEMT1k as well as a high number of pEMT3k bearing transconjugants (ca 10(6) CFU/g). In this soil the formation of a high number of 2,4-D degrading transconjugan...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/14236010" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="1633205ec34830c71772127cda116b37" rel="nofollow" data-download="{"attachment_id":44421185,"asset_id":14236010,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/44421185/download_file?st=MTczMjc0Mjc4MSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="33208391" href="https://uidaho.academia.edu/EvaTop">Eva Top</a><script data-card-contents-for-user="33208391" type="text/json">{"id":33208391,"first_name":"Eva","last_name":"Top","domain_name":"uidaho","page_name":"EvaTop","display_name":"Eva Top","profile_url":"https://uidaho.academia.edu/EvaTop?f_ri=111011","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_14236010 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="14236010"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 14236010, container: ".js-paper-rank-work_14236010", }); 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$(".js-view-count[data-work-id=14236010]").text(description); $(".js-view-count-work_14236010").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_14236010").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="14236010"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">14</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="159" href="https://www.academia.edu/Documents/in/Microbiology">Microbiology</a>, <script data-card-contents-for-ri="159" type="text/json">{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=111011","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="6947" href="https://www.academia.edu/Documents/in/Medical_Microbiology">Medical Microbiology</a>, <script data-card-contents-for-ri="6947" type="text/json">{"id":6947,"name":"Medical Microbiology","url":"https://www.academia.edu/Documents/in/Medical_Microbiology?f_ri=111011","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="83128" href="https://www.academia.edu/Documents/in/Escherichia_coli">Escherichia coli</a>, <script data-card-contents-for-ri="83128" type="text/json">{"id":83128,"name":"Escherichia coli","url":"https://www.academia.edu/Documents/in/Escherichia_coli?f_ri=111011","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="111011" href="https://www.academia.edu/Documents/in/Gene_transfer_techniques">Gene transfer techniques</a><script data-card-contents-for-ri="111011" type="text/json">{"id":111011,"name":"Gene transfer techniques","url":"https://www.academia.edu/Documents/in/Gene_transfer_techniques?f_ri=111011","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=14236010]'), work: {"id":14236010,"title":"Enhancement of 2,4-dichlorophenoxyacetic acid (2,4-D) degradation in soil by dissemination of catabolic plasmids","created_at":"2015-07-20T16:09:50.417-07:00","url":"https://www.academia.edu/14236010/Enhancement_of_2_4_dichlorophenoxyacetic_acid_2_4_D_degradation_in_soil_by_dissemination_of_catabolic_plasmids?f_ri=111011","dom_id":"work_14236010","summary":"Few studies have been done to evaluate the transfer of catabolic plasmids from an introduced donor strain to indigenous microbial populations as a means to remediate contaminated soils. In this work we determined the effect of the conjugative transfer of two 2,4-D degradative plasmids to indigenous soil bacterial populations on the rate of 2,4-D degradation in soil. We also assessed the influence of the presence of 2,4-D on the number of transconjugants formed. The two plasmids used, pEMT1k and pEMT3k, encode 2,4-D degradative genes (tfd) that differ in DNA sequence as well as gene organisation, and confer different growth rates to Ralstonia eutropha JMP228 when grown with 2,4-D as a sole carbon source. In an agricultural soil (Ardoyen) treated with 2,4-D (100 ppm) there were ca. 10(7) CFU of transconjugants per gram bearing pEMT1k as well as a high number of pEMT3k bearing transconjugants (ca 10(6) CFU/g). In this soil the formation of a high number of 2,4-D degrading transconjugan...","downloadable_attachments":[{"id":44421185,"asset_id":14236010,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":33208391,"first_name":"Eva","last_name":"Top","domain_name":"uidaho","page_name":"EvaTop","display_name":"Eva Top","profile_url":"https://uidaho.academia.edu/EvaTop?f_ri=111011","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=111011","nofollow":false},{"id":6947,"name":"Medical Microbiology","url":"https://www.academia.edu/Documents/in/Medical_Microbiology?f_ri=111011","nofollow":false},{"id":83128,"name":"Escherichia coli","url":"https://www.academia.edu/Documents/in/Escherichia_coli?f_ri=111011","nofollow":false},{"id":111011,"name":"Gene transfer techniques","url":"https://www.academia.edu/Documents/in/Gene_transfer_techniques?f_ri=111011","nofollow":false},{"id":113903,"name":"Bacteria","url":"https://www.academia.edu/Documents/in/Bacteria?f_ri=111011"},{"id":190363,"name":"Plasmids","url":"https://www.academia.edu/Documents/in/Plasmids?f_ri=111011"},{"id":223073,"name":"Herbicides","url":"https://www.academia.edu/Documents/in/Herbicides?f_ri=111011"},{"id":231547,"name":"Soil Microbiology","url":"https://www.academia.edu/Documents/in/Soil_Microbiology?f_ri=111011"},{"id":283330,"name":"Contaminated soil","url":"https://www.academia.edu/Documents/in/Contaminated_soil?f_ri=111011"},{"id":533274,"name":"Growth rate","url":"https://www.academia.edu/Documents/in/Growth_rate?f_ri=111011"},{"id":992161,"name":"Forest Soil Ecology","url":"https://www.academia.edu/Documents/in/Forest_Soil_Ecology?f_ri=111011"},{"id":1292702,"name":"Carbon Source","url":"https://www.academia.edu/Documents/in/Carbon_Source?f_ri=111011"},{"id":1537650,"name":"Agricultural Soil","url":"https://www.academia.edu/Documents/in/Agricultural_Soil?f_ri=111011"},{"id":2274872,"name":"DNA sequence","url":"https://www.academia.edu/Documents/in/DNA_sequence?f_ri=111011"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_22571330" data-work_id="22571330" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/22571330/Gene_Transfer_in_the_Lung_Using_Recombinant_Adeno_Associated_Virus">Gene Transfer in the Lung Using Recombinant Adeno-Associated Virus</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div 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href="https://www.academia.edu/Documents/in/Immunohistochemistry">Immunohistochemistry</a>, <script data-card-contents-for-ri="12071" type="text/json">{"id":12071,"name":"Immunohistochemistry","url":"https://www.academia.edu/Documents/in/Immunohistochemistry?f_ri=111011","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="84760" href="https://www.academia.edu/Documents/in/Mice">Mice</a>, <script data-card-contents-for-ri="84760" type="text/json">{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice?f_ri=111011","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="111011" href="https://www.academia.edu/Documents/in/Gene_transfer_techniques">Gene transfer techniques</a>, <script data-card-contents-for-ri="111011" type="text/json">{"id":111011,"name":"Gene transfer techniques","url":"https://www.academia.edu/Documents/in/Gene_transfer_techniques?f_ri=111011","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="197297" href="https://www.academia.edu/Documents/in/Lung">Lung</a><script data-card-contents-for-ri="197297" type="text/json">{"id":197297,"name":"Lung","url":"https://www.academia.edu/Documents/in/Lung?f_ri=111011","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=22571330]'), work: {"id":22571330,"title":"Gene Transfer in the Lung Using Recombinant Adeno-Associated Virus","created_at":"2016-02-28T20:46:26.597-08:00","url":"https://www.academia.edu/22571330/Gene_Transfer_in_the_Lung_Using_Recombinant_Adeno_Associated_Virus?f_ri=111011","dom_id":"work_22571330","summary":null,"downloadable_attachments":[{"id":43180724,"asset_id":22571330,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":44123287,"first_name":"Alisha","last_name":"Gruntman","domain_name":"independent","page_name":"AlishaGruntman","display_name":"Alisha Gruntman","profile_url":"https://independent.academia.edu/AlishaGruntman?f_ri=111011","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":12071,"name":"Immunohistochemistry","url":"https://www.academia.edu/Documents/in/Immunohistochemistry?f_ri=111011","nofollow":false},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice?f_ri=111011","nofollow":false},{"id":111011,"name":"Gene transfer techniques","url":"https://www.academia.edu/Documents/in/Gene_transfer_techniques?f_ri=111011","nofollow":false},{"id":197297,"name":"Lung","url":"https://www.academia.edu/Documents/in/Lung?f_ri=111011","nofollow":false}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_20809485" data-work_id="20809485" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/20809485/Regulated_Delivery_of_Therapeutic_Proteins_After_in_Vivo_Somatic_Cell_Gene_Transfer">Regulated Delivery of Therapeutic Proteins After in Vivo Somatic Cell Gene Transfer</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/20809485" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="7c36bebb6019eadc973822f5d5a18532" rel="nofollow" data-download="{"attachment_id":41574381,"asset_id":20809485,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/41574381/download_file?st=MTczMjc0Mjc4MSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="42099751" href="https://independent.academia.edu/GuangpingGao">Guangping Gao</a><script data-card-contents-for-user="42099751" 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class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/4356956/Methods_for_the_genetic_manipulation_of_Nonomuraea_sp_ATCC_39727">Methods for the genetic manipulation of Nonomuraea sp. ATCC 39727</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Nonomuraea sp. ATCC 39727 belongs to the Streptosporangiaceae family of filamentous actinomycetes. This microorganism produces the teicoplanin-like glycopeptide A40926, which is the starting material for the synthesis of the... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_4356956" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Nonomuraea sp. ATCC 39727 belongs to the Streptosporangiaceae family of filamentous actinomycetes. This microorganism produces the teicoplanin-like glycopeptide A40926, which is the starting material for the synthesis of the second-generation glycopeptide dalbavancin. Notwithstanding the strain’s pharmaceutical relevance, the lack or poor efficiency of genetic tools to manipulate Nonomuraea sp. ATCC 39727 has hampered strain and product improvement. Here we report the development of gene transfer systems based on protoplast transformation and intergeneric conjugation from Escherichia coli. Efficiency of transformation and conjugation, followed by site specific or homologous recombination with the Nonomuraea sp. genome, were determined using the integrative plasmid pSET152 (5.7 kb), and the Supercos1 derivative cosmid A40ΔY (30 kb). To our knowledge, this is the first report of the transformation of protoplasts of Nonomuraea sp. ATCC 39727, even though the improved procedure for intergeneric conjugation makes it the method of choice for introducing large segments of DNA into Nonomuraea sp. ATCC 39727.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/4356956" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="014505ab143da97b8b881f5b123a35d9" rel="nofollow" data-download="{"attachment_id":49917274,"asset_id":4356956,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/49917274/download_file?st=MTczMjc0Mjc4MSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="5321665" href="https://uninsubria.academia.edu/LetiziaMarcone">Letizia Marcone</a><script data-card-contents-for-user="5321665" type="text/json">{"id":5321665,"first_name":"Letizia","last_name":"Marcone","domain_name":"uninsubria","page_name":"LetiziaMarcone","display_name":"Letizia Marcone","profile_url":"https://uninsubria.academia.edu/LetiziaMarcone?f_ri=111011","photo":"https://0.academia-photos.com/5321665/25178391/23958272/s65_letizia.marcone.jpg"}</script></span></span></li><li class="js-paper-rank-work_4356956 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="4356956"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 4356956, container: ".js-paper-rank-work_4356956", }); 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ATCC 39727","created_at":"2013-08-28T22:54:25.216-07:00","url":"https://www.academia.edu/4356956/Methods_for_the_genetic_manipulation_of_Nonomuraea_sp_ATCC_39727?f_ri=111011","dom_id":"work_4356956","summary":"Nonomuraea sp. ATCC 39727 belongs to the Streptosporangiaceae family of filamentous actinomycetes. This microorganism produces the teicoplanin-like glycopeptide A40926, which is the starting material for the synthesis of the second-generation glycopeptide dalbavancin. Notwithstanding the strain’s pharmaceutical relevance, the lack or poor efficiency of genetic tools to manipulate Nonomuraea sp. ATCC 39727 has hampered strain and product improvement. Here we report the development of gene transfer systems based on protoplast transformation and intergeneric conjugation from Escherichia coli. Efficiency of transformation and conjugation, followed by site specific or homologous recombination with the Nonomuraea sp. genome, were determined using the integrative plasmid pSET152 (5.7 kb), and the Supercos1 derivative cosmid A40ΔY (30 kb). To our knowledge, this is the first report of the transformation of protoplasts of Nonomuraea sp. ATCC 39727, even though the improved procedure for intergeneric conjugation makes it the method of choice for introducing large segments of DNA into Nonomuraea sp. ATCC 39727.","downloadable_attachments":[{"id":49917274,"asset_id":4356956,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":5321665,"first_name":"Letizia","last_name":"Marcone","domain_name":"uninsubria","page_name":"LetiziaMarcone","display_name":"Letizia Marcone","profile_url":"https://uninsubria.academia.edu/LetiziaMarcone?f_ri=111011","photo":"https://0.academia-photos.com/5321665/25178391/23958272/s65_letizia.marcone.jpg"}],"research_interests":[{"id":156,"name":"Genetics","url":"https://www.academia.edu/Documents/in/Genetics?f_ri=111011","nofollow":false},{"id":14907,"name":"Industrial Biotechnology","url":"https://www.academia.edu/Documents/in/Industrial_Biotechnology?f_ri=111011","nofollow":false},{"id":41749,"name":"Genetic Engineering","url":"https://www.academia.edu/Documents/in/Genetic_Engineering?f_ri=111011","nofollow":false},{"id":64089,"name":"Genetics \u0026 Microbial 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class="u-positionAbsolute" data-has-card-for-ri-list="26140113"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">9</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="167" href="https://www.academia.edu/Documents/in/Physiology">Physiology</a>, <script data-card-contents-for-ri="167" type="text/json">{"id":167,"name":"Physiology","url":"https://www.academia.edu/Documents/in/Physiology?f_ri=111011","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="32923" href="https://www.academia.edu/Documents/in/Gene_Silencing">Gene Silencing</a>, <script data-card-contents-for-ri="32923" type="text/json">{"id":32923,"name":"Gene Silencing","url":"https://www.academia.edu/Documents/in/Gene_Silencing?f_ri=111011","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="84760" href="https://www.academia.edu/Documents/in/Mice">Mice</a>, <script data-card-contents-for-ri="84760" type="text/json">{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice?f_ri=111011","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="111011" href="https://www.academia.edu/Documents/in/Gene_transfer_techniques">Gene transfer techniques</a><script data-card-contents-for-ri="111011" type="text/json">{"id":111011,"name":"Gene transfer techniques","url":"https://www.academia.edu/Documents/in/Gene_transfer_techniques?f_ri=111011","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=26140113]'), work: {"id":26140113,"title":"Transposon-mediated transgenesis, transgenic rescue, and tissue-specific gene expression in rodents and 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Silencing","url":"https://www.academia.edu/Documents/in/Gene_Silencing?f_ri=111011","nofollow":false},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice?f_ri=111011","nofollow":false},{"id":111011,"name":"Gene transfer techniques","url":"https://www.academia.edu/Documents/in/Gene_transfer_techniques?f_ri=111011","nofollow":false},{"id":186234,"name":"Medical Physiology","url":"https://www.academia.edu/Documents/in/Medical_Physiology?f_ri=111011"},{"id":375054,"name":"Rats","url":"https://www.academia.edu/Documents/in/Rats?f_ri=111011"},{"id":788677,"name":"Rabbits","url":"https://www.academia.edu/Documents/in/Rabbits?f_ri=111011"},{"id":846443,"name":"Mosaicism","url":"https://www.academia.edu/Documents/in/Mosaicism?f_ri=111011"},{"id":1681026,"name":"Biochemistry and cell biology","url":"https://www.academia.edu/Documents/in/Biochemistry_and_cell_biology?f_ri=111011"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 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href="https://gunma-u.academia.edu/TadahiroKitamura">Tadahiro Kitamura</a><script data-card-contents-for-user="32912849" type="text/json">{"id":32912849,"first_name":"Tadahiro","last_name":"Kitamura","domain_name":"gunma-u","page_name":"TadahiroKitamura","display_name":"Tadahiro Kitamura","profile_url":"https://gunma-u.academia.edu/TadahiroKitamura?f_ri=111011","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_13815356 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="13815356"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 13815356, container: ".js-paper-rank-work_13815356", }); });</script></li><li class="js-percentile-work_13815356 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" 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data-card-contents-for-ri="71459" type="text/json">{"id":71459,"name":"Fasting","url":"https://www.academia.edu/Documents/in/Fasting?f_ri=111011","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="84760" href="https://www.academia.edu/Documents/in/Mice">Mice</a>, <script data-card-contents-for-ri="84760" type="text/json">{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice?f_ri=111011","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="111011" href="https://www.academia.edu/Documents/in/Gene_transfer_techniques">Gene transfer techniques</a><script data-card-contents-for-ri="111011" type="text/json">{"id":111011,"name":"Gene transfer techniques","url":"https://www.academia.edu/Documents/in/Gene_transfer_techniques?f_ri=111011","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=13815356]'), work: {"id":13815356,"title":"Forkhead protein FoxO1 mediates Agrp-dependent effects of leptin on food intake","created_at":"2015-07-08T15:09:25.702-07:00","url":"https://www.academia.edu/13815356/Forkhead_protein_FoxO1_mediates_Agrp_dependent_effects_of_leptin_on_food_intake?f_ri=111011","dom_id":"work_13815356","summary":null,"downloadable_attachments":[{"id":44919006,"asset_id":13815356,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":32912849,"first_name":"Tadahiro","last_name":"Kitamura","domain_name":"gunma-u","page_name":"TadahiroKitamura","display_name":"Tadahiro Kitamura","profile_url":"https://gunma-u.academia.edu/TadahiroKitamura?f_ri=111011","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":27784,"name":"Gene expression","url":"https://www.academia.edu/Documents/in/Gene_expression?f_ri=111011","nofollow":false},{"id":71459,"name":"Fasting","url":"https://www.academia.edu/Documents/in/Fasting?f_ri=111011","nofollow":false},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice?f_ri=111011","nofollow":false},{"id":111011,"name":"Gene transfer techniques","url":"https://www.academia.edu/Documents/in/Gene_transfer_techniques?f_ri=111011","nofollow":false},{"id":135357,"name":"Leptin","url":"https://www.academia.edu/Documents/in/Leptin?f_ri=111011"},{"id":177247,"name":"Arcuate Nucleus","url":"https://www.academia.edu/Documents/in/Arcuate_Nucleus?f_ri=111011"},{"id":181569,"name":"Proteins","url":"https://www.academia.edu/Documents/in/Proteins?f_ri=111011"},{"id":193974,"name":"Neurons","url":"https://www.academia.edu/Documents/in/Neurons?f_ri=111011"},{"id":213901,"name":"Transcription Factor","url":"https://www.academia.edu/Documents/in/Transcription_Factor?f_ri=111011"},{"id":218820,"name":"Eating","url":"https://www.academia.edu/Documents/in/Eating?f_ri=111011"},{"id":241044,"name":"Food intake","url":"https://www.academia.edu/Documents/in/Food_intake?f_ri=111011"},{"id":375054,"name":"Rats","url":"https://www.academia.edu/Documents/in/Rats?f_ri=111011"},{"id":1763968,"name":"Gene Expression Regulation","url":"https://www.academia.edu/Documents/in/Gene_Expression_Regulation?f_ri=111011"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_25757516" data-work_id="25757516" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/25757516/Gene_Delivery_of_a_Viral_Anti_Inflammatory_Protein_to_Combat_Ocular_Inflammation">Gene Delivery of a Viral Anti-Inflammatory Protein to Combat Ocular Inflammation</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/25757516" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="dcd1e7dc9951e128d2e5173ae9bad13e" rel="nofollow" data-download="{"attachment_id":46113478,"asset_id":25757516,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button 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Ildefonso","profile_url":"https://florida.academia.edu/CristhianIldefonso?f_ri=111011","photo":"https://0.academia-photos.com/49494963/26074832/24700813/s65_cristhian.ildefonso.jpg"}</script></span></span></li><li class="js-paper-rank-work_25757516 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="25757516"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 25757516, container: ".js-paper-rank-work_25757516", }); });</script></li><li class="js-percentile-work_25757516 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 25757516; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var 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u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="25757516"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">8</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="27784" href="https://www.academia.edu/Documents/in/Gene_expression">Gene expression</a>, <script data-card-contents-for-ri="27784" type="text/json">{"id":27784,"name":"Gene expression","url":"https://www.academia.edu/Documents/in/Gene_expression?f_ri=111011","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="84243" href="https://www.academia.edu/Documents/in/Medical_Biotechnology">Medical Biotechnology</a>, <script data-card-contents-for-ri="84243" type="text/json">{"id":84243,"name":"Medical Biotechnology","url":"https://www.academia.edu/Documents/in/Medical_Biotechnology?f_ri=111011","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="84760" href="https://www.academia.edu/Documents/in/Mice">Mice</a>, <script data-card-contents-for-ri="84760" type="text/json">{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice?f_ri=111011","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="111011" href="https://www.academia.edu/Documents/in/Gene_transfer_techniques">Gene transfer techniques</a><script data-card-contents-for-ri="111011" type="text/json">{"id":111011,"name":"Gene transfer techniques","url":"https://www.academia.edu/Documents/in/Gene_transfer_techniques?f_ri=111011","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=25757516]'), work: {"id":25757516,"title":"Gene Delivery of a Viral Anti-Inflammatory 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Biotechnology","url":"https://www.academia.edu/Documents/in/Medical_Biotechnology?f_ri=111011","nofollow":false},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice?f_ri=111011","nofollow":false},{"id":111011,"name":"Gene transfer techniques","url":"https://www.academia.edu/Documents/in/Gene_transfer_techniques?f_ri=111011","nofollow":false},{"id":129391,"name":"Gene Order","url":"https://www.academia.edu/Documents/in/Gene_Order?f_ri=111011"},{"id":244814,"name":"Clinical Sciences","url":"https://www.academia.edu/Documents/in/Clinical_Sciences?f_ri=111011"},{"id":293135,"name":"Uveitis","url":"https://www.academia.edu/Documents/in/Uveitis?f_ri=111011"},{"id":2436295,"name":"Myxoma virus","url":"https://www.academia.edu/Documents/in/Myxoma_virus?f_ri=111011"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_11396205" data-work_id="11396205" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/11396205/Enhancing_rAAV_vector_expression_in_the_lung">Enhancing rAAV vector expression in the lung</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Despite favorable DNA transfer efficiency, gene expression from recombinant adeno-associated virus (rAAV2) vectors in the lung has been variable in the context of cystic fibrosis (CF) gene therapy. This is due, in part, to the large size... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_11396205" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Despite favorable DNA transfer efficiency, gene expression from recombinant adeno-associated virus (rAAV2) vectors in the lung has been variable in the context of cystic fibrosis (CF) gene therapy. This is due, in part, to the large size of the CF transmembrane regulator (CFTR)-coding sequence which necessitates the use of compact endogenous promoter elements versus stronger exogenous promoters. We evaluated the possibility that gene expression from rAAV could be improved by using AAV capsid serotypes with greater tropism for the apical surface of airway cells (i.e. rAAV5 or rAAV1) and/or using strong promoters such as the cytomegalovirus (CMV) enhancer/chicken beta-actin hybrid (Cβ) promoter. The relative activity of the CMV immediate-early (CMVie) promoter, the Cβ promoter, and the Cβ promoter with a downstream woodchuck hepatitis virus post-transcriptional regulatory element (wpre) were assessed in vitro and in vivo in C57\Bl6 mice using human alpha-1 antitrypsin (hAAT) as a secreted reporter. In vivo, the Cβ-AAT-wpre group achieved maximum serum levels of 1.5 mg/ml of hAAT. AAV capsid serotypes were then compared in vivo utilizing the transcriptionally optimized CB-wpre cassette in rAAV serotype 1, 2 or 5 capsids (rAAV1, rAAV2, and rAAV5), utilizing luciferase as a reporter to compare expression over a wide dynamic range. The pulmonary luciferase levels at 8 weeks were similar in rAAV5 and rAAV1 groups (2.9 × 106 relative light units (RLU)/g tissue and 2.7 × 106 RLU/g tissue, respectively), both of which were much higher than rAAV2. Although the advantage of rAAV5 over rAAV2 in the lung has already been described, the availability of another serotype (rAAV1) capable of efficient gene transfer in the lung could be useful. Copyright © 2005 John Wiley & Sons, Ltd.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/11396205" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="edaff5df2d80828163debffc859a4f91" rel="nofollow" data-download="{"attachment_id":46732083,"asset_id":11396205,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/46732083/download_file?st=MTczMjc0Mjc4MSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="27716687" href="https://independent.academia.edu/KevinFoust">Kevin Foust</a><script data-card-contents-for-user="27716687" type="text/json">{"id":27716687,"first_name":"Kevin","last_name":"Foust","domain_name":"independent","page_name":"KevinFoust","display_name":"Kevin Foust","profile_url":"https://independent.academia.edu/KevinFoust?f_ri=111011","photo":"https://0.academia-photos.com/27716687/144386747/133909935/s65_kevin.foust.png"}</script></span></span></li><li class="js-paper-rank-work_11396205 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="11396205"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 11396205, container: ".js-paper-rank-work_11396205", }); 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This is due, in part, to the large size of the CF transmembrane regulator (CFTR)-coding sequence which necessitates the use of compact endogenous promoter elements versus stronger exogenous promoters. We evaluated the possibility that gene expression from rAAV could be improved by using AAV capsid serotypes with greater tropism for the apical surface of airway cells (i.e. rAAV5 or rAAV1) and/or using strong promoters such as the cytomegalovirus (CMV) enhancer/chicken beta-actin hybrid (Cβ) promoter. The relative activity of the CMV immediate-early (CMVie) promoter, the Cβ promoter, and the Cβ promoter with a downstream woodchuck hepatitis virus post-transcriptional regulatory element (wpre) were assessed in vitro and in vivo in C57\\Bl6 mice using human alpha-1 antitrypsin (hAAT) as a secreted reporter. In vivo, the Cβ-AAT-wpre group achieved maximum serum levels of 1.5 mg/ml of hAAT. AAV capsid serotypes were then compared in vivo utilizing the transcriptionally optimized CB-wpre cassette in rAAV serotype 1, 2 or 5 capsids (rAAV1, rAAV2, and rAAV5), utilizing luciferase as a reporter to compare expression over a wide dynamic range. The pulmonary luciferase levels at 8 weeks were similar in rAAV5 and rAAV1 groups (2.9 × 106 relative light units (RLU)/g tissue and 2.7 × 106 RLU/g tissue, respectively), both of which were much higher than rAAV2. Although the advantage of rAAV5 over rAAV2 in the lung has already been described, the availability of another serotype (rAAV1) capable of efficient gene transfer in the lung could be useful. Copyright © 2005 John Wiley \u0026 Sons, Ltd.","downloadable_attachments":[{"id":46732083,"asset_id":11396205,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":27716687,"first_name":"Kevin","last_name":"Foust","domain_name":"independent","page_name":"KevinFoust","display_name":"Kevin Foust","profile_url":"https://independent.academia.edu/KevinFoust?f_ri=111011","photo":"https://0.academia-photos.com/27716687/144386747/133909935/s65_kevin.foust.png"}],"research_interests":[{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice?f_ri=111011","nofollow":false},{"id":111011,"name":"Gene transfer techniques","url":"https://www.academia.edu/Documents/in/Gene_transfer_techniques?f_ri=111011","nofollow":false},{"id":161176,"name":"The","url":"https://www.academia.edu/Documents/in/The?f_ri=111011","nofollow":false},{"id":197297,"name":"Lung","url":"https://www.academia.edu/Documents/in/Lung?f_ri=111011","nofollow":false},{"id":244814,"name":"Clinical Sciences","url":"https://www.academia.edu/Documents/in/Clinical_Sciences?f_ri=111011"},{"id":295728,"name":"Molecular cloning","url":"https://www.academia.edu/Documents/in/Molecular_cloning?f_ri=111011"},{"id":413195,"name":"Time Factors","url":"https://www.academia.edu/Documents/in/Time_Factors?f_ri=111011"},{"id":620070,"name":"Transfection","url":"https://www.academia.edu/Documents/in/Transfection?f_ri=111011"},{"id":1654599,"name":"Alpha-1 Antitrypsin","url":"https://www.academia.edu/Documents/in/Alpha-1_Antitrypsin?f_ri=111011"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_67343292" data-work_id="67343292" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/67343292/The_ethical_concerns_about_transgenic_crops">The ethical concerns about transgenic crops</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">It is generally accepted that transgenesis can improve our knowledge of natural processes, but also leads to agricultural, industrial or socio-economical changes which could affect human society at large and which may, consequently,... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_67343292" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">It is generally accepted that transgenesis can improve our knowledge of natural processes, but also leads to agricultural, industrial or socio-economical changes which could affect human society at large and which may, consequently, require regulation. It is often stated that developing countries are most likely to benefit from plant biotechnology and are at the same time most likely to be affected by the deployment of such new technologies. Therefore, ethical questions related to such biotechnology probably also need to be addressed. We first illustrate how consequentialist and nonconsequentialist theories of ethics can be applied to the genetically modified organism debate, namely consequentialism, autonomy/consent ethics (i.e. self-determination of people regarding matters that may have an effect on these people) and virtue ethics (i.e. whether an action is in adequacy with ideal traits). We show that these approaches lead to highly conflicting views. We have then refocused on mo...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/67343292" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="4a3443df02bbda8dd85863d7c187eb34" rel="nofollow" data-download="{"attachment_id":78195954,"asset_id":67343292,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/78195954/download_file?st=MTczMjc0Mjc4MSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="112953140" href="https://u-psud.academia.edu/agnesRicroch">agnes Ricroch</a><script data-card-contents-for-user="112953140" type="text/json">{"id":112953140,"first_name":"agnes","last_name":"Ricroch","domain_name":"u-psud","page_name":"agnesRicroch","display_name":"agnes Ricroch","profile_url":"https://u-psud.academia.edu/agnesRicroch?f_ri=111011","photo":"https://0.academia-photos.com/112953140/26921731/25377301/s65_agnes.ricroch.jpg"}</script></span></span></li><li class="js-paper-rank-work_67343292 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="67343292"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 67343292, container: ".js-paper-rank-work_67343292", }); 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It is often stated that developing countries are most likely to benefit from plant biotechnology and are at the same time most likely to be affected by the deployment of such new technologies. Therefore, ethical questions related to such biotechnology probably also need to be addressed. We first illustrate how consequentialist and nonconsequentialist theories of ethics can be applied to the genetically modified organism debate, namely consequentialism, autonomy/consent ethics (i.e. self-determination of people regarding matters that may have an effect on these people) and virtue ethics (i.e. whether an action is in adequacy with ideal traits). We show that these approaches lead to highly conflicting views. 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Niosomes are such hydrated vesicular systems containing nonionic surfactants... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_67483329" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Vesicular systems are novel means of delivering drug in controlled manner to enhance bioavailability and get therapeutic effect over a longer period of time. Niosomes are such hydrated vesicular systems containing nonionic surfactants along with cholesterol or other lipids delivering drug to targeted site which are non toxic, requiring less production cost, stable over a longer period of time in different conditions, so overcomes drawbacks of liposome. Present review describes history, all factors affecting niosome formulation, manufacturing conditions, characterization, stability, administration routes and also their comparison with liposome. This review also gives relevant information regarding various applications of niosomes in gene delivery, vaccine delivery, anticancer drug delivery, etc.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/67483329" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="b2958ae87488d2d170238ed83ed209ba" rel="nofollow" data-download="{"attachment_id":78283237,"asset_id":67483329,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/78283237/download_file?st=MTczMjc0Mjc4MSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="154804565" href="https://independent.academia.edu/NitinMahale4">Nitin Mahale</a><script data-card-contents-for-user="154804565" type="text/json">{"id":154804565,"first_name":"Nitin","last_name":"Mahale","domain_name":"independent","page_name":"NitinMahale4","display_name":"Nitin Mahale","profile_url":"https://independent.academia.edu/NitinMahale4?f_ri=111011","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_67483329 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="67483329"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 67483329, container: ".js-paper-rank-work_67483329", }); 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action","url":"https://www.academia.edu/Documents/in/Mechanism_of_action?f_ri=111011"},{"id":772730,"name":"Charge Density","url":"https://www.academia.edu/Documents/in/Charge_Density?f_ri=111011"},{"id":1186308,"name":"Structure Determination","url":"https://www.academia.edu/Documents/in/Structure_Determination?f_ri=111011"},{"id":1232421,"name":"Physicochemical Properties","url":"https://www.academia.edu/Documents/in/Physicochemical_Properties?f_ri=111011"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_3750095" data-work_id="3750095" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/3750095/Cationic_polymers_and_their_therapeutic_potential">Cationic polymers and their therapeutic potential</a></div></div><div 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href="https://www.academia.edu/Documents/in/Survival_Analysis">Survival Analysis</a>, <script data-card-contents-for-ri="10610" type="text/json">{"id":10610,"name":"Survival Analysis","url":"https://www.academia.edu/Documents/in/Survival_Analysis?f_ri=111011","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="47884" href="https://www.academia.edu/Documents/in/Biological_Sciences">Biological Sciences</a>, <script data-card-contents-for-ri="47884" type="text/json">{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=111011","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="62692" href="https://www.academia.edu/Documents/in/Spinal_Muscular_Atrophy">Spinal Muscular Atrophy</a>, <script data-card-contents-for-ri="62692" type="text/json">{"id":62692,"name":"Spinal Muscular 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type="text/json">{"id":57802,"name":"Erythropoietin","url":"https://www.academia.edu/Documents/in/Erythropoietin?f_ri=111011","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="100732" href="https://www.academia.edu/Documents/in/Blood">Blood</a>, <script data-card-contents-for-ri="100732" type="text/json">{"id":100732,"name":"Blood","url":"https://www.academia.edu/Documents/in/Blood?f_ri=111011","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="111011" href="https://www.academia.edu/Documents/in/Gene_transfer_techniques">Gene transfer techniques</a>, <script data-card-contents-for-ri="111011" type="text/json">{"id":111011,"name":"Gene transfer techniques","url":"https://www.academia.edu/Documents/in/Gene_transfer_techniques?f_ri=111011","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="158399" href="https://www.academia.edu/Documents/in/Cyclic_peptides">Cyclic peptides</a><script 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