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<!doctype html> <html lang="fr"> <head> <title>MALDI-TOF Protocol for M. tuberculosis Identification Improv | 105075</title> <meta name="keywords" content="Bacanelli GM*, Araujo FR, Verbisck NV, "/> <meta name="description" content="Matrix Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) has been used for the identification of Mycobacterium spp. due ..105075"/> <meta name="citation_publisher" content="Longdom Publishing SL" /> <meta name="citation_journal_title" content="Maladies mycobact茅riennes"> <meta name="citation_title" content="MALDI-TOF Protocol for M. tuberculosis Identification Improved MALDI-TOF MS Identification of Mycobacterium tuberculosis by Use of a Cell Disruption Protocol"> <meta name="citation_author" content="Bacanelli GM" /> <meta name="citation_author" content="Araujo FR" /> <meta name="citation_author" content="Verbisck NV" /> <meta name="citation_year" content=""> <meta name="citation_volume" content="13"> <meta name="citation_issue" content="6"> <meta name="citation_abstract" content="Matrix Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) has been used for the identification of Mycobacterium spp. due to its speed, reliability, cost-effectiveness and high efficiency. However, the cell wall of mycobacteria is rich in lipids which makes it difficult to obtain proteins for analysis by MALDI-TOF MS. In this study, two protein extraction protocols were compared: The MycoEx, recommended by the MALDI-TOF instrument manufacturer Bruker Daltonics and the MycoLyser protocol described here in, which used the MagNA Lyser instrument for cellular rupture. Protein extraction with the two protocols was performed using the virulent strain M. tuberculosis H37Rv (NCBI NC_000962) and the results compared. The MycoLyser protocol allowed enhanced Biotyper identification of M. tuberculosis since the scores obtained with this protocol were mostly 藘1,800. The identification accuracy was 38.8% (A), 44.5% (B), 16.7% (C) for MycoLyser and 0% (A), 16.7% (B), 83.3% (C) for MycoEx. In view of these results, it is evident that the new MycoLyser protocol for mycobacterial protein extraction allows improvement of the MALDI-TOF MS method efficacy for M. tuberculosis identification."> <meta name="citation_abstract_html_url" content="https://french.longdom.org/abstract/malditof-protocol-for-emm-tuberculosisem-identification-improved-malditof-ms-identification-of-emmycobacterium-tuberculo-105075.html"> <meta name="format-detection" content="telephone=no"> <meta http-equiv="Content-Language" content="fr"> <meta name="google-site-verification" content="NomPTP94YozsgvD3NEFpNqUfY88e0TU0L64zNzZTpd0" /> <meta itemprop="name" content="longdom" /> <meta http-equiv="X-UA-Compatible" content="IE=edge"> <meta name="ROBOTS" content="INDEX,FOLLOW"> <meta name="googlebot" content="INDEX,FOLLOW"> <meta name="viewport" content="width=device-width, initial-scale=1, shrink-to-fit=no"> <link rel="canonical" 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However, the cell wall of mycobacteria is rich in lipids which makes it difficult to obtain proteins for analysis by MALDI-TOF MS. In this study, two protein extraction protocols were compared: The MycoEx, recommended by the MALDI-TOF instrument manufacturer Bruker Daltonics and the MycoLyser protocol described here in, which used the MagNA Lyser instrument for cellular rupture. Protein extraction with the two protocols was performed using the virulent strain M. tuberculosis H37Rv (NCBI NC_000962) and the results compared. The MycoLyser protocol allowed enhanced Biotyper identification of M. tuberculosis since the scores obtained with this protocol were mostly 藘1,800. The identification accuracy was 38.8% (A), 44.5% (B), 16.7% (C) for MycoLyser and 0% (A), 16.7% (B), 83.3% (C) for MycoEx. In view of these results, it is evident that the new MycoLyser protocol for mycobacterial protein extraction allows improvement of the MALDI-TOF MS method efficacy for M. tuberculosis identification.</p> <div class="alert alert-info text-left"><b>Clause de non-responsabilit茅:</b> Ce r茅sum茅 a 茅t茅 traduit 脿 l'aide d'outils d'intelligence artificielle et n'a pas encore 茅t茅 r茅vis茅 ou v茅rifi茅.</div> <div class="nav social-icons"> <a class="nav-link w-auto">Partagez cet article</a> <a title="Cliquez ici" target="_blank" href="https://www.facebook.com/sharer.php?u=https://french.longdom.org/abstract/malditof-protocol-for-emm-tuberculosisem-identification-improved-malditof-ms-identification-of-emmycobacterium-tuberculo-105075.html" rel="noopener"><i class="fab fa-facebook-f"></i></a> <a class="nav-link" title="Cliquez ici" target="_blank" href="https://twitter.com/share?url=https://french.longdom.org/abstract/malditof-protocol-for-emm-tuberculosisem-identification-improved-malditof-ms-identification-of-emmycobacterium-tuberculo-105075.html" rel="noopener"><i class="fab fa-twitter"></i></a> <a class="nav-link" title="Cliquez ici" target="_blank" href="https://www.linkedin.com/shareArticle?mini=true&url=https://french.longdom.org/abstract/malditof-protocol-for-emm-tuberculosisem-identification-improved-malditof-ms-identification-of-emmycobacterium-tuberculo-105075.html" rel="noopener"><i class="fab fa-linkedin-in"></i></a> <a class="nav-link" title="Cliquez ici" target="_blank" href="https://plus.google.com/share?url=https://french.longdom.org/abstract/malditof-protocol-for-emm-tuberculosisem-identification-improved-malditof-ms-identification-of-emmycobacterium-tuberculo-105075.html" rel="noopener"><i class="fab fa-google-plus-g"></i></a> </div> </div> </div> </div> </section> <footer class="bg-blue-grey-900 py-3"> <div class="container"> <div class="row"> <div class="col-12 col-sm-4"> <h4 class="white font-size-4 fweight-400 border-bottom-1 pb-2">Liens de contenu</h4> <ul class="list-unstyled footer-links font-size-3"> <li><a class="" href="https://french.longdom.org/privacy-policy.html" title="Cliquez ici">politique de confidentialit茅</a></li> <li><a class="" href="https://french.longdom.org/terms-conditions.html" title="Cliquez ici">termes et conditions</a></li> <li><a class="" href="https://french.longdom.org/authors-reviewers-editors.html" title="Cliquez ici">Auteurs, critiques et 茅diteur</a></li> </ul> </div> <div class="col-12 col-sm-4"> <h4 class="white font-size-4 fweight-400 border-bottom-1 pb-2">Contacter Longdom</h4> <p><strong>Groupe Longdom SA</strong><br /> Avenue Roger Vandendriessche,<br /> 18, 1150 Bruxelles, Belgique<br /> T茅l茅phone : +442038085340<br /> <strong>Courriel :</strong> <a class="white" href="mailto:info@longdom.org" title="Cliquez ici">info@longdom.org</a></p> </div> <div class="col-12 col-sm-4"> <h4 class="white font-size-4 fweight-400 border-bottom-1 pb-2">Connecter</h4> <nav class="nav nav-pills social-icons-footer flex-column a-pl-0"> <a href="https://www.facebook.com/longdompublisher" title="Cliquez ici" target="_blank" class="nav-link bg-facebook-hover"><i class="fab fa-facebook-f bg-facebook"></i></a> <a href="https://www.linkedin.com/company/longdom-publishing-sl/" title="Cliquez ici" target="_blank" class="nav-link bg-linkedin-hover"><i class="fab fa-linkedin-in bg-linkedin"></i></a> <a href="https://twitter.com/LongdomP" title="Cliquez ici" target="_blank" class="nav-link bg-twitter-hover"><i class="fab fa-twitter bg-twitter"></i></a> <a href="https://www.instagram.com/longdom_publisher/" title="Cliquez ici" target="_blank" class="nav-link bg-instagram-hover"><i class="fab fa-instagram bg-instagram"></i></a> </nav> </div> </div> <div class="row text-center"> <div class="col"> <p>droits d auteur © 2024 <a href="https://french.longdom.org/" title="Cliquez ici" class="white">Longdom 脡ditions SL</a>.</p> </div> </div> </div> </footer>  <a href="#0" class="cd-top js-cd-top">Top</a>   <script src="https://code.jquery.com/jquery-3.3.1.min.js"></script> <script src="https://cdnjs.cloudflare.com/ajax/libs/popper.js/1.14.7/umd/popper.min.js"></script> <script src="https://stackpath.bootstrapcdn.com/bootstrap/4.3.1/js/bootstrap.min.js"></script>  <script> jQuery(function($) { $(window).scroll(function fix_element() { $('#target').css( $(window).scrollTop() > 100 ? 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