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(PDF) Phytochemical, cytotoxic and antibacterial activity of two medicinal plants of Bangladesh

<!DOCTYPE html> <html > <head> <meta charset="utf-8"> <meta rel="search" type="application/opensearchdescription+xml" href="/open_search.xml" title="Academia.edu"> <meta content="width=device-width, initial-scale=1" name="viewport"> <meta name="google-site-verification" content="bKJMBZA7E43xhDOopFZkssMMkBRjvYERV-NaN4R6mrs"> <meta name="csrf-param" content="authenticity_token" /> <meta name="csrf-token" content="NVh0SRhRe0Sv4xs_HHaIbOfSj9YDZKaCZeYUxRDD6pkgAFjTuTiP3Q70s25ltzbtXP3l0ShdvVwtoqZ7XxOrTg" /> <meta name="citation_title" content="Phytochemical, cytotoxic and antibacterial activity of two medicinal plants of Bangladesh" /> <meta name="citation_journal_title" content="Pharmacologyonline" /> <meta name="citation_author" content="Amina Khatun" /> <meta name="citation_author" content="Mahmudur Rahman" /> <meta name="twitter:card" content="summary" /> <meta name="twitter:url" content="https://www.academia.edu/17375438/Phytochemical_cytotoxic_and_antibacterial_activity_of_two_medicinal_plants_of_Bangladesh" /> <meta name="twitter:title" content="Phytochemical, cytotoxic and antibacterial activity of two medicinal plants of Bangladesh" /> <meta name="twitter:description" content="Ethanol extracts of the traditionally valuable plant Jasminum grandiflorum L. (Family – Oleaceae) and Ficus religiosa L. (Family – Moraceae) were screened for cytotoxicity and antibacterial activity as well as for some important phytochemical groups." /> <meta name="twitter:image" content="http://a.academia-assets.com/images/twitter-card.jpeg" /> <meta property="fb:app_id" content="2369844204" /> <meta property="og:type" content="article" /> <meta property="og:url" content="https://www.academia.edu/17375438/Phytochemical_cytotoxic_and_antibacterial_activity_of_two_medicinal_plants_of_Bangladesh" /> <meta property="og:title" content="Phytochemical, cytotoxic and antibacterial activity of two medicinal plants of Bangladesh" /> <meta property="og:image" content="http://a.academia-assets.com/images/open-graph-icons/fb-paper.gif" /> <meta property="og:description" content="Ethanol extracts of the traditionally valuable plant Jasminum grandiflorum L. (Family – Oleaceae) and Ficus religiosa L. (Family – Moraceae) were screened for cytotoxicity and antibacterial activity as well as for some important phytochemical groups." /> <meta property="article:author" content="https://manarat.academia.edu/AminaKhatun" /> <meta property="article:author" content="https://scu-au.academia.edu/MahmudurRahman" /> <meta name="description" content="Ethanol extracts of the traditionally valuable plant Jasminum grandiflorum L. (Family – Oleaceae) and Ficus religiosa L. (Family – Moraceae) were screened for cytotoxicity and antibacterial activity as well as for some important phytochemical groups." /> <title>(PDF) Phytochemical, cytotoxic and antibacterial activity of two medicinal plants of Bangladesh</title> <link rel="canonical" href="https://www.academia.edu/17375438/Phytochemical_cytotoxic_and_antibacterial_activity_of_two_medicinal_plants_of_Bangladesh" /> <script async src="https://www.googletagmanager.com/gtag/js?id=G-5VKX33P2DS"></script> <script> window.dataLayer = window.dataLayer || []; function gtag(){dataLayer.push(arguments);} gtag('js', new Date()); gtag('config', 'G-5VKX33P2DS', { cookie_domain: 'academia.edu', send_page_view: false, }); gtag('event', 'page_view', { 'controller': "single_work", 'action': "show", 'controller_action': 'single_work#show', 'logged_in': 'false', 'edge': 'unknown', // Send nil if there is no A/B test bucket, in case some records get logged // with missing data - that way we can distinguish between the two cases. // ab_test_bucket should be of the form <ab_test_name>:<bucket> 'ab_test_bucket': null, }) </script> <script> var $controller_name = 'single_work'; var $action_name = "show"; var $rails_env = 'production'; var $app_rev = '17dc1d026887981e26e513d2e4ccfc15439ba3f9'; var $domain = 'academia.edu'; var $app_host = "academia.edu"; var $asset_host = "academia-assets.com"; var $start_time = new Date().getTime(); var $recaptcha_key = "6LdxlRMTAAAAADnu_zyLhLg0YF9uACwz78shpjJB"; var $recaptcha_invisible_key = "6Lf3KHUUAAAAACggoMpmGJdQDtiyrjVlvGJ6BbAj"; var $disableClientRecordHit = false; </script> <script> window.require = { config: function() { return function() {} } } </script> <script> window.Aedu = window.Aedu || {}; window.Aedu.hit_data = null; window.Aedu.serverRenderTime = new Date(1739914613000); window.Aedu.timeDifference = new Date().getTime() - 1739914613000; </script> <script type="application/ld+json">{"@context":"https://schema.org","@type":"ScholarlyArticle","abstract":"Ethanol extracts of the traditionally valuable plant Jasminum grandiflorum L. (Family – Oleaceae) and Ficus religiosa L. (Family – Moraceae) were screened for cytotoxicity and antibacterial activity as well as for some important phytochemical groups. The dried extracts of J. grandiflorum and F. religiosa were dissolved in 99.8% ethanol and qualitatively analysed for alkaloids, glycosides, steroids, gums, reducing sugars, tannins, flavonoids, and saponins. Cytotoxic potential of dimethyl sulfoxide solution of the extracts were measured by using in vivo brine shrimp lethality assay. LC50 and LC90 values were determined graphically by plotting mortality against concentrations. Agar disc diffusion method was used to determine antibacterial sensitivity of the extracts against selected 14 pathogenic bacteria. Minimum inhibitory concentration was determined by broth macro dilution assay. The percent mortality of shrimp was increased with the increase of the doses of the extracts. 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(Family – Oleaceae) and Ficus religiosa L. (Family – Moraceae) were screened for cytotoxicity and antibacterial activity as well as for some important phytochemical groups. The dried extracts of J. grandiflorum and F. religiosa were dissolved in 99.8% ethanol and qualitatively analysed for alkaloids, glycosides, steroids, gums, reducing sugars, tannins, flavonoids, and saponins. Cytotoxic potential of dimethyl sulfoxide solution of the extracts were measured by using in vivo brine shrimp lethality assay. LC50 and LC90 values were determined graphically by plotting mortality against concentrations. Agar disc diffusion method was used to determine antibacterial sensitivity of the extracts against selected 14 pathogenic bacteria. Minimum inhibitory concentration was determined by broth macro dilution assay. The percent mortality of shrimp was increased with the increase of the doses of the extracts. LC50 and ...","publication_name":"Pharmacologyonline"},"document_type":"paper","pre_hit_view_count_baseline":null,"quality":"high","language":"en","title":"Phytochemical, cytotoxic and antibacterial activity of two medicinal plants of Bangladesh","broadcastable":true,"draft":null,"has_indexable_attachment":true,"indexable":true}}["work"]; window.loswp.workCoauthors = [7411416,1411297]; window.loswp.locale = "en"; window.loswp.countryCode = "SG"; window.loswp.cwvAbTestBucket = ""; window.loswp.designVariant = "ds_vanilla"; window.loswp.fullPageMobileSutdModalVariant = "control"; window.loswp.useOptimizedScribd4genScript = false; window.loginModal = {}; window.loginModal.appleClientId = 'edu.academia.applesignon'; window.userInChina = "false";</script><script defer="" src="https://accounts.google.com/gsi/client"></script><div class="ds-loswp-container"><div class="ds-work-card--grid-container"><div class="ds-work-card--container js-loswp-work-card"><div class="ds-work-card--cover"><div class="ds-work-cover--wrapper"><div class="ds-work-cover--container"><button class="ds-work-cover--clickable js-swp-download-button" data-signup-modal="{&quot;location&quot;:&quot;swp-splash-paper-cover&quot;,&quot;attachmentId&quot;:39474144,&quot;attachmentType&quot;:&quot;pdf&quot;}"><img alt="First page of “Phytochemical, cytotoxic and antibacterial activity of two medicinal plants of Bangladesh”" class="ds-work-cover--cover-thumbnail" src="https://0.academia-photos.com/attachment_thumbnails/39474144/mini_magick20190222-10432-k1w5zu.png?1550894516" /><img alt="PDF Icon" class="ds-work-cover--file-icon" src="//a.academia-assets.com/images/single_work_splash/adobe_icon.svg" /><div class="ds-work-cover--hover-container"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">download</span><p>Download Free PDF</p></div><div class="ds-work-cover--ribbon-container">Download Free PDF</div><div class="ds-work-cover--ribbon-triangle"></div></button></div></div></div><div class="ds-work-card--work-information"><h1 class="ds-work-card--work-title">Phytochemical, cytotoxic and antibacterial activity of two medicinal plants of Bangladesh</h1><div class="ds-work-card--work-authors ds-work-card--detail"><a class="ds-work-card--author js-wsj-grid-card-author ds2-5-body-md ds2-5-body-link" data-author-id="7411416" href="https://manarat.academia.edu/AminaKhatun"><img alt="Profile image of Amina Khatun" class="ds-work-card--author-avatar" src="//a.academia-assets.com/images/s65_no_pic.png" />Amina Khatun</a><a class="ds-work-card--author js-wsj-grid-card-author ds2-5-body-md ds2-5-body-link" data-author-id="1411297" href="https://scu-au.academia.edu/MahmudurRahman"><img alt="Profile image of Mahmudur Rahman" class="ds-work-card--author-avatar" src="https://0.academia-photos.com/1411297/2598737/3018650/s65_mahmudur.rahman.jpg" />Mahmudur Rahman</a></div><div class="ds-work-card--detail"><p class="ds-work-card--detail ds2-5-body-sm">Pharmacologyonline</p><div class="ds-work-card--work-metadata"><div class="ds-work-card--work-metadata__stat"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">visibility</span><p class="ds2-5-body-sm" id="work-metadata-view-count">…</p></div><div class="ds-work-card--work-metadata__stat"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">description</span><p class="ds2-5-body-sm">8 pages</p></div><div class="ds-work-card--work-metadata__stat"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">link</span><p class="ds2-5-body-sm">1 file</p></div></div><script>(async () => { const workId = 17375438; 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(Family – Oleaceae) and Ficus religiosa L. (Family – Moraceae) were screened for cytotoxicity and antibacterial activity as well as for some important phytochemical groups. The dried extracts of J. grandiflorum and F. religiosa were dissolved in 99.8% ethanol and qualitatively analysed for alkaloids, glycosides, steroids, gums, reducing sugars, tannins, flavonoids, and saponins. Cytotoxic potential of dimethyl sulfoxide solution of the extracts were measured by using in vivo brine shrimp lethality assay. LC50 and LC90 values were determined graphically by plotting mortality against concentrations. Agar disc diffusion method was used to determine antibacterial sensitivity of the extracts against selected 14 pathogenic bacteria. Minimum inhibitory concentration was determined by broth macro dilution assay. The percent mortality of shrimp was increased with the increase of the doses of the extracts. LC50 and ...</p><div class="ds-work-card--button-container"><button class="ds2-5-button js-swp-download-button" data-signup-modal="{&quot;location&quot;:&quot;continue-reading-button--work-card&quot;,&quot;attachmentId&quot;:39474144,&quot;attachmentType&quot;:&quot;pdf&quot;,&quot;workUrl&quot;:&quot;https://www.academia.edu/17375438/Phytochemical_cytotoxic_and_antibacterial_activity_of_two_medicinal_plants_of_Bangladesh&quot;}">See full PDF</button><button class="ds2-5-button ds2-5-button--secondary js-swp-download-button" data-signup-modal="{&quot;location&quot;:&quot;download-pdf-button--work-card&quot;,&quot;attachmentId&quot;:39474144,&quot;attachmentType&quot;:&quot;pdf&quot;,&quot;workUrl&quot;:&quot;https://www.academia.edu/17375438/Phytochemical_cytotoxic_and_antibacterial_activity_of_two_medicinal_plants_of_Bangladesh&quot;}"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">download</span>Download PDF</button></div><div class="ds-signup-banner-trigger-container"><div class="ds-signup-banner-trigger ds-signup-banner-trigger-control"></div></div><div class="ds-signup-banner ds-signup-banner-control"><div id="ds-signup-banner-close-button"><button class="ds2-5-button ds2-5-button--secondary ds2-5-button--inverse"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">close</span></button></div><div class="ds-signup-banner-ctas"><img src="//a.academia-assets.com/images/academia-logo-capital-white.svg" /><h4 class="ds2-5-heading-serif-sm">Sign up for access to the world's latest research</h4><button class="ds2-5-button ds2-5-button--inverse ds2-5-button--full-width js-swp-download-button" data-signup-modal="{&quot;location&quot;:&quot;signup-banner&quot;}">Sign up for free<span class="material-symbols-outlined" style="font-size: 20px" translate="no">arrow_forward</span></button></div><div class="ds-signup-banner-divider"></div><div class="ds-signup-banner-reasons"><div class="ds-signup-banner-reasons-item"><span class="material-symbols-outlined" style="font-size: 24px" translate="no">check</span><span>Get notified about relevant papers</span></div><div class="ds-signup-banner-reasons-item"><span class="material-symbols-outlined" style="font-size: 24px" translate="no">check</span><span>Save papers to use in your research</span></div><div class="ds-signup-banner-reasons-item"><span class="material-symbols-outlined" style="font-size: 24px" translate="no">check</span><span>Join the discussion with peers</span></div><div class="ds-signup-banner-reasons-item"><span class="material-symbols-outlined" style="font-size: 24px" translate="no">check</span><span>Track your impact</span></div></div></div><script>(() => { // Set up signup banner show/hide behavior: // 1. 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The crude methanolic extract of S. japonica, n-hexane, chloroform and ethyl acetate soluble fractions of methanolic extract were screened for their antimicrobial activity against a wide range of both Gram-positive and Gram-negative bacteria by disc diffusion method. The crude extract showed moderate and n-Hexane, chloroform soluble fraction of crude extract showed mild antibacterial activity against both Gram-positive and Gram-negative bacteria. Ethyl acetate soluble fraction of the extract showed significant antimicrobial activity against Salmonella typhi, Escherichia coli and Bacillus cereus. The zones of inhibition produced by the crude methanolic extract, n-hexane, chloroform and ethyl acetate soluble fractions were found to be 12.80-16.55 mm, 12.60 mm, 5-14.30 mm and 10-20.25 mm, respectively, at a concentration of 30 g/disc. Chloroform, n-hexane and ethyl acetate soluble fractions of methanolic extract of S. japonica were screened for antitumor properties using brine shrimp lethality bioassay. A reputed cytotoxic agent vincristine sulphate was used as a positive control. From the results of the brine shrimp lethality bioassay, it can be well predicted that chloroform and ethyl acetate soluble fractions of methanolic extract of S. japonica possess cytotoxic principles (with LC 50 66.488 mg/ml and LC 50 45.662 mg/ml, respectively) comparison with positive control vincristine sulphate (with LC 50 0.839 mg/ml). But n-hexane soluble fractions of methanolic extract of S. japonica exhibited no lethality effect on shrimp nauplii.</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{&quot;location&quot;:&quot;wsj-grid-card-download-pdf-modal&quot;,&quot;work_title&quot;:&quot;The Antimicrobial activity and Brine Shrimp Lethality Bioassay of Leaf extracts of Stephania japonica (Akanadi)&quot;,&quot;attachmentId&quot;:37153574,&quot;attachmentType&quot;:&quot;pdf&quot;,&quot;work_url&quot;:&quot;https://www.academia.edu/11715302/The_Antimicrobial_activity_and_Brine_Shrimp_Lethality_Bioassay_of_Leaf_extracts_of_Stephania_japonica_Akanadi_&quot;,&quot;alternativeTracking&quot;:true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/11715302/The_Antimicrobial_activity_and_Brine_Shrimp_Lethality_Bioassay_of_Leaf_extracts_of_Stephania_japonica_Akanadi_"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="1" data-entity-id="56751228" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/56751228/Phytochemical_analysis_and_broad_spectrum_antimicrobial_activity_of_ethanolic_extract_of_Jasminum_mesnyi_Hance_leaves_and_its_solvent_partitioned_fractions">Phytochemical analysis and broad spectrum antimicrobial activity of ethanolic extract of Jasminum mesnyi Hance leaves and its solvent partitioned fractions</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="1128246" href="https://independent.academia.edu/BSBalaji">BS Balaji</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Bioinformation</p><p class="ds-related-work--abstract ds2-5-body-sm">Jasminum mesnyi Hance (yellow jasmine, Family Oleaceae) belongs to an important subclass of Jasminum family whose biological significance is under explored. The current study pertains to isolation of various solvent extracts fractions and their anti-bacterial effects on the Gram-positive and Gram-negative bacterial pathogens. Ethanolic extract of J. mesnyi Hance leaves was subjected to further partitioning using different solvents with increasing polarity to get different solvent fractions. Different extracts were analysed for their phytochemical constitutents and were evaluated for their antibacterial activity against a number of diseases causing bacteria. Diethylether fraction (DEF) showed remarkable inhibition of growth of Aeromonas hydrophila and Vibrio parahaemolyticus with inhibition zones of 17 and 19mm, respectively at 250 µg. On the other hand, the hexane fraction (HF) was found to be more effective against Gram-positive test bacterial pathogens (Bacillus anthracis and Bacillus subtilis) with inhibition zones of 19.6 mm and 17.5 mm, respectively. The phytochemical screening of different test fractions revealed the predominant presence of cardiac glycosides, sterols and terpenoids in DEF and HF, while Ethylacetate fraction (EAF) and methanol fractions (MF) were found rich in flavonoids and phenols with moderate amount of other reference metabolites.</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{&quot;location&quot;:&quot;wsj-grid-card-download-pdf-modal&quot;,&quot;work_title&quot;:&quot;Phytochemical analysis and broad spectrum antimicrobial activity of ethanolic extract of Jasminum mesnyi Hance leaves and its solvent partitioned fractions&quot;,&quot;attachmentId&quot;:71981948,&quot;attachmentType&quot;:&quot;pdf&quot;,&quot;work_url&quot;:&quot;https://www.academia.edu/56751228/Phytochemical_analysis_and_broad_spectrum_antimicrobial_activity_of_ethanolic_extract_of_Jasminum_mesnyi_Hance_leaves_and_its_solvent_partitioned_fractions&quot;,&quot;alternativeTracking&quot;:true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/56751228/Phytochemical_analysis_and_broad_spectrum_antimicrobial_activity_of_ethanolic_extract_of_Jasminum_mesnyi_Hance_leaves_and_its_solvent_partitioned_fractions"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="2" data-entity-id="58565721" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/58565721/Chemical_Composition_Antimicrobial_Properties_and_Toxicity_of_Jatropha_curcas_Provenances_from_Diverse_Origins">Chemical Composition, Antimicrobial Properties and Toxicity of Jatropha curcas Provenances from Diverse Origins</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="6609901" href="https://independent.academia.edu/JeanetteMadas">Jeanette Madas</a></div><p class="ds-related-work--metadata ds2-5-body-xs">International Journal of Agriculture and Biology</p><p class="ds-related-work--abstract ds2-5-body-sm">This paper discusses probable therapeutic bases of 14 Jatropha curcas provenances based on their cytotoxicity, secondary metabolite profile and antimicrobial activity. Ethanolic extracts of Jatropha leaves and bark were subjected to antimicrobial and brine shrimp toxicity assays. The India 2 provenance showed a broad range of antibacterial activity against Escherichia coli, Staphylococcus aureus and Bacillus cereus. The rest of the provenances also revealed varying degrees of antibacterial activity against the three bacteria except for provenances India 1, Phil 6 and Tubang bakod, which did not show antimicrobial action. Brine shrimp lethality assay revealed high toxicity levels for the different Jatropha extracts. The presence of alkaloids, flavonoids, leucoanthocyanins, saponins, tannins and phenolics was detected through phytochemical analysis. The results of various assays indicate that Jatropha provenances have the potential for the development of novel compounds for the treatm...</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{&quot;location&quot;:&quot;wsj-grid-card-download-pdf-modal&quot;,&quot;work_title&quot;:&quot;Chemical Composition, Antimicrobial Properties and Toxicity of Jatropha curcas Provenances from Diverse Origins&quot;,&quot;attachmentId&quot;:72916679,&quot;attachmentType&quot;:&quot;pdf&quot;,&quot;work_url&quot;:&quot;https://www.academia.edu/58565721/Chemical_Composition_Antimicrobial_Properties_and_Toxicity_of_Jatropha_curcas_Provenances_from_Diverse_Origins&quot;,&quot;alternativeTracking&quot;:true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/58565721/Chemical_Composition_Antimicrobial_Properties_and_Toxicity_of_Jatropha_curcas_Provenances_from_Diverse_Origins"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="3" data-entity-id="89003556" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/89003556/Antibacterial_Antidiabetic_and_Brine_Shrimp_Lethality_Activities_of_Some_Selected_Medicinal_Plants_from_Kavrepalanchok_District_of_Nepal">Antibacterial, Antidiabetic and Brine Shrimp Lethality Activities of Some Selected Medicinal Plants from Kavrepalanchok District of Nepal</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="30037680" href="https://independent.academia.edu/KhagarajSharma">Khagaraj Sharma</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Journal of Institute of Science and Technology, 2019</p><p class="ds-related-work--abstract ds2-5-body-sm">The methanol extracts of nine medicinal plants collected from Kavrepalanchok district of Nepal were subjected to their potential bioactivity viz. antibacterial, antidiabetic and toxicity tests. Antibacterial property of the extracts was evaluated against Gram-positive and Gram-negative bacteria by agar well diffusion method and antidiabetic activity was investigated by α-amylase inhibition assay. The toxicity of plant extracts was assessed by brine shrimp lethality test. All the nine different plant extracts showed antibacterial activity with the zone of inhibition (ZOI) ranging from 5 to 35 mm. Among the studied plant extracts, Psidium guajava showed the highest ZOI against Salmonella typhi (35 mm) while Melia azedarach was most effective against Staphylococcus aureus (22 mm). The percentage of α- amylase enzyme inhibition was found up to 88.56 ± 3.50 in dose dependent manner. The enzyme inhibitory concentration IC50 value of standard, acarbose was 166.01 μg/mL while the most effec...</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{&quot;location&quot;:&quot;wsj-grid-card-download-pdf-modal&quot;,&quot;work_title&quot;:&quot;Antibacterial, Antidiabetic and Brine Shrimp Lethality Activities of Some Selected Medicinal Plants from Kavrepalanchok District of Nepal&quot;,&quot;attachmentId&quot;:92881159,&quot;attachmentType&quot;:&quot;pdf&quot;,&quot;work_url&quot;:&quot;https://www.academia.edu/89003556/Antibacterial_Antidiabetic_and_Brine_Shrimp_Lethality_Activities_of_Some_Selected_Medicinal_Plants_from_Kavrepalanchok_District_of_Nepal&quot;,&quot;alternativeTracking&quot;:true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/89003556/Antibacterial_Antidiabetic_and_Brine_Shrimp_Lethality_Activities_of_Some_Selected_Medicinal_Plants_from_Kavrepalanchok_District_of_Nepal"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="4" data-entity-id="3177152" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/3177152/Assessment_of_bioactivity_of_Indian_medicinal_plants_using_brine_shrimp_Artemia_salina_lethality_assay">Assessment of bioactivity of Indian medicinal plants using brine shrimp (Artemia salina) lethality assay</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="3628684" href="https://independent.academia.edu/BensonOluwafemi">Benson Oluwafemi</a></div><p class="ds-related-work--metadata ds2-5-body-xs">2005</p><p class="ds-related-work--abstract ds2-5-body-sm">Medicinal plants constitute an important component of flora and are widely distributed in India. The pharmacological evaluation of substances from plants is an established method for the identification of lead compounds which can leads to the development of novel and safe medicinal agents. Based on the ethnopharmacological literature, several species of medicinal plants used in traditional medicine in India were collected.</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{&quot;location&quot;:&quot;wsj-grid-card-download-pdf-modal&quot;,&quot;work_title&quot;:&quot;Assessment of bioactivity of Indian medicinal plants using brine shrimp (Artemia salina) lethality assay&quot;,&quot;attachmentId&quot;:31068189,&quot;attachmentType&quot;:&quot;pdf&quot;,&quot;work_url&quot;:&quot;https://www.academia.edu/3177152/Assessment_of_bioactivity_of_Indian_medicinal_plants_using_brine_shrimp_Artemia_salina_lethality_assay&quot;,&quot;alternativeTracking&quot;:true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/3177152/Assessment_of_bioactivity_of_Indian_medicinal_plants_using_brine_shrimp_Artemia_salina_lethality_assay"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="5" data-entity-id="65935377" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/65935377/Evaluation_of_Antibacterial_Activity_of_Phytoconstituents_Isolated_from_Jasminum_sambac_L_and_their_Identification_through_GC_MS">Evaluation of Antibacterial Activity of Phytoconstituents Isolated from Jasminum sambac L. and their Identification through GC-MS</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="17718381" href="https://independent.academia.edu/ShilpiRijhwani">Shilpi Rijhwani</a></div><p class="ds-related-work--metadata ds2-5-body-xs">2015</p><p class="ds-related-work--abstract ds2-5-body-sm">The present study was initiated to explore the antibacterial activity of Jasminum sambac L.in view of its pharmacological utilization in ancient as well as modern system of medicine. Leaves and stem of Jasminum sambac L. were extracted in various solvents ranging from nonpolar to polar, viz., nhexane, chloroform, ethyl acetate, methanol and water. All the plant extracts were screened for antibacterial activity against selected ATCC bacterial strains. Few plant extracts were found to show high antibacterial activity. However, for the present study we have taken into consideration only the leaf and stem extracts obtained in ethyl acetate. Both plant extracts were further evaluated for the identification of phytoconstituents by GC-MS.</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{&quot;location&quot;:&quot;wsj-grid-card-download-pdf-modal&quot;,&quot;work_title&quot;:&quot;Evaluation of Antibacterial Activity of Phytoconstituents Isolated from Jasminum sambac L. and their Identification through GC-MS&quot;,&quot;attachmentId&quot;:77319078,&quot;attachmentType&quot;:&quot;pdf&quot;,&quot;work_url&quot;:&quot;https://www.academia.edu/65935377/Evaluation_of_Antibacterial_Activity_of_Phytoconstituents_Isolated_from_Jasminum_sambac_L_and_their_Identification_through_GC_MS&quot;,&quot;alternativeTracking&quot;:true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/65935377/Evaluation_of_Antibacterial_Activity_of_Phytoconstituents_Isolated_from_Jasminum_sambac_L_and_their_Identification_through_GC_MS"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="6" data-entity-id="66156543" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/66156543/Evaluation_of_Phytochemicals_Found_in_the_Leaves_of_Jasminum_sambac_L_for_Antimicrobial_Activity">Evaluation of Phytochemicals Found in the Leaves of Jasminum sambac L. for Antimicrobial Activity</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="13182631" href="https://independent.academia.edu/kamakshitomar">kamakshi tomar</a></div><p class="ds-related-work--metadata ds2-5-body-xs">2020</p><p class="ds-related-work--abstract ds2-5-body-sm">Jasminum sambac Linn. (Family—Oleaceae) was tested for antibacterial activity against three important pathogenic bacteria, i.e., Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. Powdered leaf material was extracted with different solvents from non-polar to polar viz., n-hexane, chloroform, ethyl acetate, methanol, and water using Soxhlet apparatus. All the solvent extracts were evaporated to dryness using a hot dry air oven. The dry residue was dissolved in respective mother solvents (1:10 w/v) and tested for the presence or absence of various phytochemicals. An antimicrobial test was also followed to check the antimicrobial activity. Among five solvents tested, methanol, n-hexane, and ethyl acetate extracts showed significant antibacterial activity and chloroform extract showed less activity when compared with control (respective mother solvent). Water extract did not show any activity against all three microorganisms.</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{&quot;location&quot;:&quot;wsj-grid-card-download-pdf-modal&quot;,&quot;work_title&quot;:&quot;Evaluation of Phytochemicals Found in the Leaves of Jasminum sambac L. for Antimicrobial Activity&quot;,&quot;attachmentId&quot;:77459831,&quot;attachmentType&quot;:&quot;pdf&quot;,&quot;work_url&quot;:&quot;https://www.academia.edu/66156543/Evaluation_of_Phytochemicals_Found_in_the_Leaves_of_Jasminum_sambac_L_for_Antimicrobial_Activity&quot;,&quot;alternativeTracking&quot;:true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/66156543/Evaluation_of_Phytochemicals_Found_in_the_Leaves_of_Jasminum_sambac_L_for_Antimicrobial_Activity"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="7" data-entity-id="99567930" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/99567930/Antimicrobial_phytochemical_and_larvicidal_properties_of_Jatropha_multifida_Linn">Antimicrobial, phytochemical and larvicidal properties of Jatropha multifida Linn</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="59478856" href="https://independent.academia.edu/SillmaRampadarath">Sillma Rampadarath</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Asian Pacific Journal of Tropical Medicine, 2014</p><p class="ds-related-work--abstract ds2-5-body-sm">To evaluate the phytochemical, antimicrobial and insecticidal properties of different extracts of Jatropha multifida (J. multifida) in Mauritius. Methods: Qualitative and quantitative methods were used for the determination of the presence of phytochemicals in the crude solvent extracts of J. multifida. The antimicrobial sensitivity (disc diffusion method) and antibacterial activity (microdilution method) of 13 microorganisms were reported. The insecticidal properties of the crude solvent extracts were tested against the larvae of two insects, Bactrocera zonata and Bactrocera cucurbitae (Diptera: Tephritidae), which cause important economic losses to local fruits. Results: Ethyl acetate was proved to be a good solvent for extraction. J. multifida showed very interesting activity against Bacillus algicola and Staphylococcus epidermis. The plant also showed good larvacidal activity against Bactrocera zonata. Conclusions: This paper reports the dual use of J. multifida, hence further studies can be made in term of application.</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{&quot;location&quot;:&quot;wsj-grid-card-download-pdf-modal&quot;,&quot;work_title&quot;:&quot;Antimicrobial, phytochemical and larvicidal properties of Jatropha multifida Linn&quot;,&quot;attachmentId&quot;:100621770,&quot;attachmentType&quot;:&quot;pdf&quot;,&quot;work_url&quot;:&quot;https://www.academia.edu/99567930/Antimicrobial_phytochemical_and_larvicidal_properties_of_Jatropha_multifida_Linn&quot;,&quot;alternativeTracking&quot;:true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/99567930/Antimicrobial_phytochemical_and_larvicidal_properties_of_Jatropha_multifida_Linn"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="8" data-entity-id="65836881" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/65836881/Screening_of_phytochemical_properties_and_antimicrobial_activity_of_Malaysian_medicinal_plants_against_aquatic_bacteria">Screening of phytochemical properties and antimicrobial activity of Malaysian medicinal plants against aquatic bacteria</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="167343665" href="https://independent.academia.edu/RainaManaf">Raina Manaf</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Malaysian Journal of Microbiology</p><p class="ds-related-work--abstract ds2-5-body-sm">Aims: Quantitative screening of phytochemical properties and antimicrobial activities were done on some plants of importance in human medicine as traditional herbs to treat fish diseases in Malaysia. Six extracts of Vitex trifolia, Aloe vera, Strobilanthes crispus, Clinacanthus nutans, Pereskia grandifolia and Peperomia pellucida were determined for phytochemical properties and their antibacterial activities against common freshwater pathogens i.e. Streptococcus agalactiae, Aeromonas hydrophila and Enterobacter cloacae. Methodology and results: Qualitative screening of phytochemical properties in herbs were determined using conversional method for flavonoids, tannins, saponin, alkaloids steroid and glycoside The results showed flavonoid was presence in all plant extracts. For the antimicrobial activity, the aqueous and methanolic extracts were tested by using disk diffusion method. Antimicrobial assay of methanolic crude extracts (25 to 100 mg/mL) showed effectiveness against the pathogenic bacteria. Comparatively, all aqueous extracts did not show any antimicrobial activity. Strong antibacterial activity was shown by the methanolic extracts of V. trifolia, A. vera and S. crispus while moderate antimicrobial activity was shown by C. nutans, P. grandifolia and P. pellucida. Conclusion, significance and impact study: The current results indicated that the studied plants might indeed be potential sources of natural antimicrobial agents to control fish diseases.</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{&quot;location&quot;:&quot;wsj-grid-card-download-pdf-modal&quot;,&quot;work_title&quot;:&quot;Screening of phytochemical properties and antimicrobial activity of Malaysian medicinal plants against aquatic bacteria&quot;,&quot;attachmentId&quot;:77260824,&quot;attachmentType&quot;:&quot;pdf&quot;,&quot;work_url&quot;:&quot;https://www.academia.edu/65836881/Screening_of_phytochemical_properties_and_antimicrobial_activity_of_Malaysian_medicinal_plants_against_aquatic_bacteria&quot;,&quot;alternativeTracking&quot;:true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/65836881/Screening_of_phytochemical_properties_and_antimicrobial_activity_of_Malaysian_medicinal_plants_against_aquatic_bacteria"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="9" data-entity-id="29388882" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/29388882/In_vitro_Scientific_evaluation_on_antimicrobial_antioxidant_cytotoxic_properties_and_phytochemical_constituents_of_traditional_coastal_medicinal_plants">In vitro—Scientific evaluation on antimicrobial, antioxidant, cytotoxic properties and phytochemical constituents of traditional coastal medicinal plants</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="2822885" href="https://agri.academia.edu/SriRamkumarVijayan">Sri Ramkumar Vijayan</a></div><p class="ds-related-work--abstract ds2-5-body-sm">In the present study, evaluation of antimicrobial, antioxidant, phytochemical constituents and toxicological properties of six coastal medicinal plants (CMP&#39;s) such as Ipomea biloba, Cantharanthus roseus, Cymbopogon citratus, Vitex negundo, Thespesia populnea and Pandanus tectorius was done. The maximum antimicrobial activity was recorded by methanolic extracts of V. negundo and T. populnea against bacterial and fungal pathogens. Similarly, methanolic extracts of V. negundo and T. populnea evidenced highest antioxidant properties. The extract of T. populnea showed the maximum cytotoxicity against Artemia salina with the LC 50 value of 478.11 mg/ml. The hemolytic property of CMP&#39;s extracts was V. negundo (8.91%), T. populnea (21%) and C. citratus (64%) also the hemolytic index did not show any hemolysis of human erythrocytes. Furthermore, the TLC separation of V. negundo and T. populnea extracts exhibited the presence of Flavonoids (Rf-0.74) and Terpenoids (Rf-0.64). The present findings propose the promising clinical applications of selected CMP&#39;s.</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{&quot;location&quot;:&quot;wsj-grid-card-download-pdf-modal&quot;,&quot;work_title&quot;:&quot;In vitro—Scientific evaluation on antimicrobial, antioxidant, cytotoxic properties and phytochemical constituents of traditional coastal medicinal plants&quot;,&quot;attachmentId&quot;:49830507,&quot;attachmentType&quot;:&quot;pdf&quot;,&quot;work_url&quot;:&quot;https://www.academia.edu/29388882/In_vitro_Scientific_evaluation_on_antimicrobial_antioxidant_cytotoxic_properties_and_phytochemical_constituents_of_traditional_coastal_medicinal_plants&quot;,&quot;alternativeTracking&quot;:true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/29388882/In_vitro_Scientific_evaluation_on_antimicrobial_antioxidant_cytotoxic_properties_and_phytochemical_constituents_of_traditional_coastal_medicinal_plants"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div></div></div><div class="ds-sticky-ctas--wrapper js-loswp-sticky-ctas hidden"><div class="ds-sticky-ctas--grid-container"><div class="ds-sticky-ctas--container"><button class="ds2-5-button js-swp-download-button" data-signup-modal="{&quot;location&quot;:&quot;continue-reading-button--sticky-ctas&quot;,&quot;attachmentId&quot;:39474144,&quot;attachmentType&quot;:&quot;pdf&quot;,&quot;workUrl&quot;:null}">See full PDF</button><button class="ds2-5-button ds2-5-button--secondary js-swp-download-button" data-signup-modal="{&quot;location&quot;:&quot;download-pdf-button--sticky-ctas&quot;,&quot;attachmentId&quot;:39474144,&quot;attachmentType&quot;:&quot;pdf&quot;,&quot;workUrl&quot;:null}"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">download</span>Download PDF</button></div></div></div><div class="ds-below-fold--grid-container"><div class="ds-work--container js-loswp-embedded-document"><div class="attachment_preview" data-attachment="Attachment_39474144" style="display: none"><div class="js-scribd-document-container"><div class="scribd--document-loading js-scribd-document-loader" style="display: block;"><img alt="Loading..." src="//a.academia-assets.com/images/loaders/paper-load.gif" /><p>Loading Preview</p></div></div><div style="text-align: center;"><div class="scribd--no-preview-alert js-preview-unavailable"><p>Sorry, preview is currently unavailable. 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