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Protein Bioprocessing - Discovery | Bio-Techne
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class="dropdown-item text-wrap" data-drupal-link-system-path="node/531">Single Cell Western</a> <a href="/instruments" class="dropdown-item text-wrap" data-drupal-link-system-path="node/6731">View All Instruments</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Applications<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/methods/bioprocessing" class="dropdown-item text-wrap">Bioprocessing</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Research Areas<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/research-areas/cell-and-gene-therapy" class="dropdown-item text-wrap" data-drupal-link-system-path="node/51">Cell & Gene Therapy</a> <a href="/applications/immuno-oncology-clinical" class="dropdown-item text-wrap" 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Controls</a> <a href="/diagnostics/controls-reagents/rnascope-ish-probe-high-risk-hpv" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5981">RNAscope™ ISH Probe High Risk HPV</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Kits<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/diagnostics/amplidex-genetic-testing-kits" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5581">Genetics</a> <a href="/diagnostics/quantidex-oncology-testing-kits" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5586">Oncology</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Technology<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/diagnostics/exosome" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5486">Exosome Platform</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> OEM Services<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/diagnostics/ivd-assay-development" class="dropdown-item text-wrap" data-drupal-link-system-path="node/10881">IVD Assay Development</a> <a href="/diagnostics/ivd-contract-manufacturing" class="dropdown-item text-wrap" data-drupal-link-system-path="node/7381">IVD Contract Manufacturing</a> <a href="/diagnostics/molecular-quality-controls/oem-and-commercial-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/6781">Molecular Diagnostic OEM Services</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Companion Diagnostics<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/diagnostics/companion-diagnostics" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5631">Companion Diagnostics Services</a> <a href="/diagnostics/companion-diagnostics/multiomics" class="dropdown-item text-wrap" data-drupal-link-system-path="node/10616">Multiomics Platform</a> </div> </div> </div> </div> </div> </li> <li class="nav-item dropdown"> <a class="nav-link dropdown-toggle" href="#" id="navbarDropdown-services" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false"> Services <span class="icon-bt-icon-caret-cropped menu-caret js-caret d-inline-block"></span> </a> <div class="dropdown-menu dropdown-menu-services" aria-labelledby="navbarDropdown-services"> <div class="row"> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Custom Quotes<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/services/bulk-quotes" class="dropdown-item text-wrap" data-drupal-link-system-path="services/bulk-quotes">Bulk Quote Discounts</a> <a href="/services/custom-formulation" class="dropdown-item text-wrap" data-drupal-link-system-path="services/custom-formulation">Custom Formulation Quotes</a> <a href="/reagents/rnascope-ish-technology/probes/custom" class="dropdown-item text-wrap" data-drupal-link-system-path="node/12571">RNAscope Custom Probe Design Quote</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Custom Services<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/services/custom-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/1891">All Custom Services</a> <a href="/services/custom-antibody-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/906">Custom Antibody Services</a> <a href="/services/custom-cell-and-gene-therapy-services" class="dropdown-item text-wrap" 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href="/services/instrument-services/simple-western-service-plans" class="dropdown-item text-wrap" data-drupal-link-system-path="node/3181">Simple Western Service Plans</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Licensing<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/services/biotechnology-licensing-partnering" class="dropdown-item text-wrap" data-drupal-link-system-path="node/1101">Biotechnology Licensing and Partnering</a> </div> </div> </div> </div> </div> </li> <li class="nav-item dropdown"> <a class="nav-link dropdown-toggle" href="#" id="navbarDropdown-resources" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false"> Resources <span class="icon-bt-icon-caret-cropped menu-caret js-caret d-inline-block"></span> </a> <div class="dropdown-menu dropdown-menu-resources" aria-labelledby="navbarDropdown-resources"> <div class="row"> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Product & Instrument Tools<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/resources/cofa-finder-tool" class="dropdown-item text-wrap" data-drupal-link-system-path="resources/cofa-finder-tool">Certificate of Analysis (COA) Finder Tool</a> <a href="/resources/calculators" class="dropdown-item text-wrap" data-drupal-link-system-path="node/7416">Calculators</a> <a href="/resources/flow-cytometry-panel-builder" class="dropdown-item text-wrap" data-drupal-link-system-path="node/8821">Flow Cytometry Panel Builder</a> <a href="/luminex-assay-customization-tool" class="dropdown-item text-wrap" data-drupal-link-system-path="luminex-assay-customization-tool">Luminex Assay Customization Tool</a> <a href="/resources/product-insert-lookup-tool" class="dropdown-item text-wrap" data-drupal-link-system-path="resources/product-insert-lookup-tool">Product Insert Lookup 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When screening candidates and profiling immune responses can be done rapidly and effectively in the discovery stages, your downstream processes become quicker, cheaper and simpler.</p></div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_indv_media"> <div> <img src="https://resources.bio-techne.com/images/webinar/LP_images/Protein-Bioprocessing-LP-Discovery-Immune-Cell.png" alt="Bio-techne can help you fast-track your discovery stages with automated and innovative solutions." loading="lazy" > </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--_-2-media-to-text mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_media_to_text:field_indv_media"> <div> <img src="https://resources.bio-techne.com/images/webinar/LP_images/Protein-Bioprocessing-LP-Products-Luminex-v2.png" alt="Luminex instruments and assays for use in protein bioprocessing workflows, immunology research and more" loading="lazy" > </div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_media_to_text:field_header_2_global"> <div><h2>Multiplex with Luminex<sup>®</sup></h2></div> </div> <div class="field_block:paragraph:2_2_media_to_text:field_lg_sngl_frmtd_text_global"> <div><p>Understanding how the immune system responds to your candidate therapeutic can make or break its success.</p> <p>The earlier you have this data, the better - but running multiple assays for all your candidate therapeutics can take a lot of your sample, time and resources.</p> <p>Discover a better way with ready-to-run and customizable Luminex cytokine panels. Save sample, time and money with quick, comprehensive immune profiling comparable to conventional assays – like <a href="/reagents/elisa-kits">ELISA</a> and qPCR – with greater efficiency and throughput.</p></div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--_-2-text-to-media mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_lg_sngl_frmtd_text_global"> <div><h2><a name="Simple-Western"></a>Simple Screening with Simple Western</h2> <p>Screening candidates is a key step in protein discovery – but the semi-quantitative, labor-intensive, and time-consuming nature of Western blotting can let you down.</p> <p>Let our <a href="/instruments/simple-western">Simple Western™</a> platforms bring you back up! For super-fast, high-throughput antibody screening with quantitative results you can rely on, Simple Western technology is the simple choice.</p> <p>The early bird catches the worm. Get ahead of your competition in the early stages of your workflow - rapidly perform charge distribution of mAbs, antibody screening, clone selection product titer, identity, and degradation analyses with Simple Western.</p></div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/protein-bioprocessing-lp-products-v5.png" alt="ProteinSimple Simple Western Instruments" loading="lazy" > </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--card-paragraph paragraph--view-mode--default card-para-default"> <div class="row card_row"> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/p/simple-western/jess_004-650" > <div class="teaser-card-image-crop mb-3"> <img loading="lazy" class="card-background-image card-img-top" src="https://resources.bio-techne.com/images/webinar/LP_images/Protein-Bioprocessing-LP-Products-Background-v2.png" /> </div> <div class="card-foreground-image"> <img src="https://resources.bio-techne.com/images/webinar/LP_images/Protein-Bioprocessing-LP-Products-Jess.png" alt="ProteinSimple instrument Jess for chemiluminescence and fluorescence multiplexing." loading="lazy" > </div> </a> <a class="card_title_link" href="/p/simple-western/jess_004-650" > <h3 class="card_title_htag"> <div><h3>Jess</h3></div> </h3> </a> <div class="card_description flex-grow-1"> <div><p><strong>Simple Western size assays using chemiluminescence or fluorescence</strong></p> <p>Multiplex protein quantification with best-in-class detection sensitivity.</p> <ul> <li>Fully automated solution for up to 25 samples</li> <li>Fully analyzed results in just 3 hours using just 3 µL of starting material</li> <li>Multiplex using NIR/IR fluorescence and chemiluminescence</li> <li>Sequential immunoassays or total protein detection on the same sample with RePlex</li> </ul></div> </div> <div class="card-bottom-link"> <div> <a href="/p/simple-western/jess_004-650" > Meet Jess </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/p/simple-western/abby_004-680" > <div class="teaser-card-image-crop mb-3"> <img loading="lazy" class="card-background-image card-img-top" src="https://resources.bio-techne.com/images/webinar/LP_images/Protein-Bioprocessing-LP-Products-Background-v2.png" /> </div> <div class="card-foreground-image"> <img src="https://resources.bio-techne.com/images/webinar/LP_images/abby-product-v3.png" alt="ProteinSimple instrument Abby for chemiluminescence assays." loading="lazy" > </div> </a> <a class="card_title_link" href="/p/simple-western/abby_004-680" > <h3 class="card_title_htag"> <div><h3>Abby</h3></div> </h3> </a> <div class="card_description flex-grow-1"> <div><p><strong>Simple Western size assays using chemiluminescence</strong></p> <p>Abby quickly gets you down to pg-level sensitivity with just 3 µL of starting material.</p> <ul> <li>Fully automated solution</li> <li>Process 25 samples in just 3 hours</li> <li>Get up to 6 logs of dynamic range with HDR detection</li> <li>RePlex lets you perform a second round of immunoassay or total protein detection in the same capillary</li> </ul></div> </div> <div class="card-bottom-link"> <div> <a href="/p/simple-western/abby_004-680" > Meet Abby </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/instruments/single-cell-western" > <div class="teaser-card-image-crop mb-3"> <img loading="lazy" class="card-background-image card-img-top" src="https://resources.bio-techne.com/images/webinar/LP_images/Protein-Bioprocessing-LP-Products-Background-v2.png" /> </div> <div class="card-foreground-image"> <img src="https://resources.bio-techne.com/images/webinar/LP_images/Protein-Bioprocessing-LP-Products-Milo.png" alt="Milo performs Western analysis on individual cells to provide insight into population heterogeneity" loading="lazy" > </div> </a> <a class="card_title_link" href="/instruments/single-cell-western" > <h3 class="card_title_htag"> <div><h3>Milo</h3></div> </h3> </a> <div class="card_description flex-grow-1"> <div><p><strong>Single-Cell Westerns</strong></p> <p>Welcome to the world of Single-Cell Westerns!</p> <ul> <li>Step away from population level resolution of Western blotting and off-target binding of flow cytometry, and embrace the Single-Cell Western revolution</li> <li>Resolve single-cell heterogeneity in thousands of cells in just one run!</li> </ul></div> </div> <div class="card-bottom-link"> <div> <a href="/instruments/single-cell-western" > Meet Milo </a> </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--card-paragraph paragraph--view-mode--default card-para-default"> <div class="row card_row"> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/p/simple-western/sally-sue_004-700" > <div class="teaser-card-image-crop mb-3"> <img loading="lazy" class="card-background-image card-img-top" src="https://resources.bio-techne.com/images/webinar/LP_images/Protein-Bioprocessing-LP-Products-Background-v2.png" /> </div> <div class="card-foreground-image"> <img src="https://resources.bio-techne.com/images/webinar/LP_images/Protein-Bioprocessing-LP-Products-Sally-Sue.png" alt="ProteinSimple instrument Sally Sue for high-throughput Simple Western size assays." loading="lazy" > </div> </a> <a class="card_title_link" href="/p/simple-western/sally-sue_004-700" > <h3 class="card_title_htag"> <div><h3>Sally Sue</h3></div> </h3> </a> <div class="card_description flex-grow-1"> <div><p><strong>High throughput Simple Western size assays using chemiluminescence</strong></p> <p>Sally Sue provides you with a fast and simple method to characterize proteins by size in up to 96 samples at a time.</p> <ul> <li>Process 96 samples overnight</li> <li>Separate and analyze proteins between 2 kDa and 440 kDa</li> <li>Input as little as 0.2 µg/µL of protein in just 5 µL of sample</li> <li>Immunoassay or total protein analysis</li> </ul></div> </div> <div class="card-bottom-link"> <div> <a href="/p/simple-western/sally-sue_004-700" > Meet Sally Sue </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/p/simple-western/peggy-sue_004-800" > <div class="teaser-card-image-crop mb-3"> <img loading="lazy" class="card-background-image card-img-top" src="https://resources.bio-techne.com/images/webinar/LP_images/Protein-Bioprocessing-LP-Products-Background-v2.png" /> </div> <div class="card-foreground-image"> <img src="https://resources.bio-techne.com/images/webinar/LP_images/Protein-Bioprocessing-LP-Products-Peggy-Sue.png" alt="ProteinSimple instrument Peggy Sue for high-throughput charge assays." loading="lazy" > </div> </a> <a class="card_title_link" href="/p/simple-western/peggy-sue_004-800" > <h3 class="card_title_htag"> <div><h3>Peggy Sue</h3></div> </h3> </a> <div class="card_description flex-grow-1"> <div><p><strong>High throughput Simple Western size and charge assays using chemiluminescence</strong></p> <p>Peggy Sue provides you with a fast and simple method to characterize proteins by size or charge in up to 96 samples at a time.</p> <ul> <li>Separate and analyze proteins by size from 2-440 kDa or by charge from pI 3-10</li> <li>Process 96 samples overnight</li> <li>Input as little as 0.2 µg/µL of protein in just 5 µL of sample</li> <li>Immunoassay or total protein analysis</li> </ul></div> </div> <div class="card-bottom-link"> <div> <a href="/p/simple-western/peggy-sue_004-800" > Meet Peggy Sue </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/p/simple-western/nanopro-1000_004-109" > <div class="teaser-card-image-crop mb-3"> <img loading="lazy" class="card-background-image card-img-top" src="https://resources.bio-techne.com/images/webinar/LP_images/Protein-Bioprocessing-LP-Products-Background-v2.png" /> </div> <div class="card-foreground-image"> <img src="https://resources.bio-techne.com/images/webinar/LP_images/Protein-Bioprocessing-LP-Products-NanoPro.png" alt="ProteinSimple instrument Milo for Single-Cell Westerns." loading="lazy" > </div> </a> <a class="card_title_link" href="/p/simple-western/nanopro-1000_004-109" > <h3 class="card_title_htag"> <div><h3>NanoPro 1000</h3></div> </h3> </a> <div class="card_description flex-grow-1"> <div><p><strong>Simple Western charge assays with chemiluminescence</strong></p> <p>NanoPro 1000 can give you all the charge heterogeneity details on your protein in as few as 25 cells and up to 96 samples at a time.</p> <ul> <li>Process 96 samples overnight</li> <li>Fully automated solution</li> <li>Separate and analyze proteins between pI 3-10</li> <li>Resolve protein isoforms like phosphorylated and glycosylated proteins in complex samples</li> </ul></div> </div> <div class="card-bottom-link"> <div> <a href="/p/simple-western/nanopro-1000_004-109" > Meet NanoPro 1000 </a> </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph-image-banner banner_wrapper"> <div class="banner_background_image" data-bgimage="https://resources.bio-techne.com/images/webinar/LP_images/Protein-Bioprocessing-LP-Clone-Selection-Background-Verify-v3.png" style="background-repeat: no-repeat"> <div class="text_align_left"> <div><h2>Check Out the Full Range of Simple Western Certified Antibodies Available in our Database</h2></div> <a href="/resources/simple-western-antibody-database" > <button type="button" class="btn btn-primary"> Find out more </button> </a> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><h2>Take Charge Of Discovery</h2> <p>Monitoring post-translational modifications through charge heterogeneity and IgG analysis of your protein therapeutic candidate is an important quality requirement for regulatory authorities. But, what if you could easily and quickly obtain this information during the discovery stages? You could make early, yet informed decisions on candidates – saving you precious time downstream.</p> <p><strong>Reproducible results in under 35 minutes!</strong></p> <p>Discover how to get quantitative analysis of identity, purity and heterogeneity profiles of biopharmaceuticals in a snap!</p></div> </div> </div> <div> <div class="paragraph paragraph--type--card-paragraph paragraph--view-mode--default card-para-default"> <div class="row card_row"> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/p/imaged-capillary-electrophoresis-ice/maurice-c_090-002" > <div class="teaser-card-image-crop mb-3"> <img loading="lazy" class="card-background-image card-img-top" src="https://resources.bio-techne.com/images/webinar/LP_images/Protein-Bioprocessing-LP-Products-Background-v2.png" /> </div> <div class="card-foreground-image"> <img src="https://resources.bio-techne.com/images/webinar/LP_images/Protein-Bioprocessing-LP-Products-MauriceC.png" alt="maurice c" loading="lazy" > </div> </a> <a class="card_title_link" href="/p/imaged-capillary-electrophoresis-ice/maurice-c_090-002" > <h3 class="card_title_htag"> <div>Meet Maurice C</div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>How about quantitative pl and charge heterogeneity analysis in less than 10 minutes?</p></div> </div> <div class="card-bottom-link"> <div> <a href="/p/imaged-capillary-electrophoresis-ice/maurice-c_090-002" > Get cIEF data with Maurice C. </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/p/imaged-capillary-electrophoresis-ice/maurice-s_090-001" > <div class="teaser-card-image-crop mb-3"> <img loading="lazy" class="card-background-image card-img-top" src="https://resources.bio-techne.com/images/webinar/LP_images/Protein-Bioprocessing-LP-Products-Background-v2.png" /> </div> <div class="card-foreground-image"> <img src="https://resources.bio-techne.com/images/webinar/LP_images/Protein-Bioprocessing-LP-Products-MauriceS.png" alt="maurice s" loading="lazy" > </div> </a> <a class="card_title_link" href="/p/imaged-capillary-electrophoresis-ice/maurice-s_090-001" > <h3 class="card_title_htag"> <div>Meet Maurice S</div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>Getting IgG purity analysis that meets regulatory requirements needn’t be laborious. 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