Simple Western Automated Western Blot Systems

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Areas</a> </div> </div> </div> </div> </div> </li> <li class="nav-item dropdown"> <a class="nav-link dropdown-toggle" href="#" id="navbarDropdown-therapeutic-manufacturing" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false"> Therapeutic Manufacturing <span class="icon-bt-icon-caret-cropped menu-caret js-caret d-inline-block"></span> </a> <div class="dropdown-menu dropdown-menu-therapeutic-manufacturing" aria-labelledby="navbarDropdown-therapeutic-manufacturing"> <div class="row"> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Reagents & Kits<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/reagents/cell-culture-reagents/serum-free-animal-free" class="dropdown-item text-wrap" data-drupal-link-system-path="node/3576">Animal-Free Cell Culture</a> <a href="/reagents/elisa-kits" class="dropdown-item text-wrap" data-drupal-link-system-path="node/176">ELISA Kits</a> <a 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href="/gmp-products/ruo-to-gmp" class="dropdown-item text-wrap" data-drupal-link-system-path="node/3421">Transition to GMP </a> <a href="/gmp-products/loa-request" class="dropdown-item text-wrap" data-drupal-link-system-path="node/13326">DMF/LOA Request</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Instruments<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/brands/proteinsimple" class="dropdown-item text-wrap" data-drupal-link-system-path="node/3281">ProteinSimple Instruments</a> <a href="/instruments/ice" class="dropdown-item text-wrap" data-drupal-link-system-path="node/256">iCE Maurice</a> <a href="/instruments/imagers" class="dropdown-item text-wrap" data-drupal-link-system-path="node/536">Imagers</a> <a href="/instruments/luminex" class="dropdown-item text-wrap" data-drupal-link-system-path="node/241">Luminex</a> <a href="/instruments/micro-flow-imaging" class="dropdown-item text-wrap" data-drupal-link-system-path="node/526">Micro-Flow Imaging</a> <a href="/instruments/simple-plex" class="dropdown-item text-wrap" data-drupal-link-system-path="node/541">Simple Plex Ella</a> <a href="/instruments/simple-western" class="dropdown-item text-wrap is-active" data-drupal-link-system-path="node/521" aria-current="page">Simple Western</a> <a href="/instruments/single-cell-dispensers" class="dropdown-item text-wrap" data-drupal-link-system-path="node/6361">Single Cell Dispensers</a> <a href="/instruments/single-cell-western" class="dropdown-item text-wrap" data-drupal-link-system-path="node/531">Single Cell Western</a> <a href="/instruments" class="dropdown-item text-wrap" data-drupal-link-system-path="node/6731">View All Instruments</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Applications<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/methods/bioprocessing" class="dropdown-item text-wrap">Bioprocessing</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Research Areas<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/research-areas/cell-and-gene-therapy" class="dropdown-item text-wrap" data-drupal-link-system-path="node/51">Cell & Gene Therapy</a> <a href="/applications/immuno-oncology-clinical" class="dropdown-item text-wrap" data-drupal-link-system-path="node/2066">Immuno-Oncology</a> </div> </div> </div> </div> </div> </li> <li class="nav-item dropdown"> <a class="nav-link dropdown-toggle" href="#" id="navbarDropdown-diagnostics" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false"> Diagnostics <span class="icon-bt-icon-caret-cropped menu-caret js-caret d-inline-block"></span> </a> <div class="dropdown-menu dropdown-menu-diagnostics" aria-labelledby="navbarDropdown-diagnostics"> <div class="row"> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Controls & Reagents<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/diagnostics/clinical-quality-controls" class="dropdown-item text-wrap" data-drupal-link-system-path="node/7606">Clinical Quality Controls</a> <a href="/diagnostics/molecular-quality-controls" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5596">Molecular Controls</a> <a href="/diagnostics/controls-reagents/rnascope-ish-probe-high-risk-hpv" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5981">RNAscope™ ISH Probe High Risk HPV</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Kits<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/diagnostics/amplidex-genetic-testing-kits" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5581">Genetics</a> <a href="/diagnostics/quantidex-oncology-testing-kits" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5586">Oncology</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Technology<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/diagnostics/exosome" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5486">Exosome Platform</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> OEM Services<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/diagnostics/ivd-assay-development" class="dropdown-item text-wrap" data-drupal-link-system-path="node/10881">IVD Assay Development</a> <a href="/diagnostics/ivd-contract-manufacturing" class="dropdown-item text-wrap" data-drupal-link-system-path="node/7381">IVD Contract Manufacturing</a> <a href="/diagnostics/molecular-quality-controls/oem-and-commercial-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/6781">Molecular Diagnostic OEM Services</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Companion Diagnostics<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/diagnostics/companion-diagnostics" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5631">Companion Diagnostics Services</a> <a href="/diagnostics/companion-diagnostics/multiomics" class="dropdown-item text-wrap" data-drupal-link-system-path="node/10616">Multiomics Platform</a> </div> </div> </div> </div> </div> </li> <li class="nav-item dropdown"> <a class="nav-link dropdown-toggle" href="#" id="navbarDropdown-services" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false"> Services <span class="icon-bt-icon-caret-cropped menu-caret js-caret d-inline-block"></span> </a> <div class="dropdown-menu dropdown-menu-services" aria-labelledby="navbarDropdown-services"> <div class="row"> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Custom Quotes<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/services/bulk-quotes" class="dropdown-item text-wrap" data-drupal-link-system-path="services/bulk-quotes">Bulk Quote Discounts</a> <a href="/services/custom-formulation" class="dropdown-item text-wrap" data-drupal-link-system-path="services/custom-formulation">Custom Formulation Quotes</a> <a href="/reagents/rnascope-ish-technology/probes/custom" class="dropdown-item text-wrap" data-drupal-link-system-path="node/12571">RNAscope Custom Probe Design Quote</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Custom 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Bio-Techne" data-drupal-link-system-path="node/1136">Custom Gene Engineering Services</a> <a href="/services/custom-luminex-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/271">Custom Luminex Services</a> <a href="/services/custom-protein-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/1441">Custom Protein Services</a> <a href="/services/custom-simple-plex-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/6296">Custom Simple Plex Services</a> <a href="/services/custom-targeted-protein-degradation-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/861">Custom Targeted Protein Degradation Services</a> <a href="/services/professional-assay-services-rnascope" class="dropdown-item text-wrap" data-drupal-link-system-path="node/12306">RNAscope Professional Assay Services</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Instrument Services<span 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</div> </li> <li class="nav-item dropdown"> <a class="nav-link dropdown-toggle" href="#" id="navbarDropdown-resources" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false"> Resources <span class="icon-bt-icon-caret-cropped menu-caret js-caret d-inline-block"></span> </a> <div class="dropdown-menu dropdown-menu-resources" aria-labelledby="navbarDropdown-resources"> <div class="row"> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Product & Instrument Tools<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/resources/cofa-finder-tool" class="dropdown-item text-wrap" data-drupal-link-system-path="resources/cofa-finder-tool">Certificate of Analysis (COA) Finder Tool</a> <a href="/resources/calculators" class="dropdown-item text-wrap" data-drupal-link-system-path="node/7416">Calculators</a> <a href="/resources/flow-cytometry-panel-builder" class="dropdown-item text-wrap" data-drupal-link-system-path="node/8821">Flow Cytometry Panel Builder</a> <a href="/luminex-assay-customization-tool" class="dropdown-item text-wrap" data-drupal-link-system-path="luminex-assay-customization-tool">Luminex Assay Customization Tool</a> <a href="/resources/product-insert-lookup-tool" class="dropdown-item text-wrap" data-drupal-link-system-path="resources/product-insert-lookup-tool">Product Insert Lookup Tool</a> <a href="/resources/rnascope-citations" class="dropdown-item text-wrap" data-drupal-link-system-path="resources/rnascope-citations">RNAscope Citations</a> <a href="/resources/simple-plex-panel-builder" class="dropdown-item text-wrap" data-drupal-link-system-path="resources/simple-plex-panel-builder">Simple Plex Panel Builder</a> <a href="/resources/simple-western-kit-builder" class="dropdown-item text-wrap" data-drupal-link-system-path="resources/simple-western-kit-builder">Simple Western Kit Builder</a> <a href="/resources/simple-western-antibody-database" 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<img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/obt-simple-western-hero-480x480-v3.png" alt="Simple Western Jess & Leo instruments" loading="lazy" > </div> </div> </div> </div> </div> </div> </div> </div> </div> </section> </div> </div> </header> </div> <div id="main" class="container-fluid container-wide"> <div class="row"> <div class="col-md-12"> <div> <div id="block-bio-techne-global-breadcrumbs" class="system_breadcrumb_block"> <nav role="navigation" aria-labelledby="system-breadcrumb"> <h2 id="system-breadcrumb" class="visually-hidden">Breadcrumb</h2> <ol> <li> <a href="/">Home</a> </li> <li> <a href="/instruments">Instruments</a> </li> <li> Simple Western </li> </ol> </nav> </div> </div> </div> </div> <main> <section class="section"> <div> <div id="block-bio-techne-global-content" class="system_main_block"> <article> <div> <div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><a href="/instruments/simple-western/models">Compare Models</a> | <a href="/instruments/simple-western/simple-western-assays">Consumables</a> | <a href="/resources/instrument-software-download-center/compass-software-simple-western">Software</a> | <a href="/resources/simple-western-antibody-database">Antibody Database</a></p></div> </div> </div> <div> <div class="paragraph paragraph--type--section paragraph--view-mode--default mb-4"> <div> <div> <div class="paragraph paragraph--type--_-2-text-to-media mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_header_2_global"> <div><h2>What is Simple Western?</h2></div> </div> <div class="field_block:paragraph:2_2_text_to_media:field_lg_sngl_frmtd_text_global"> <div><p>Simple Western™ Technology provides automated capillary western analysis using capillary electrophoresis for protein separation followed by immunoassay-based detection within a fully enclosed system. Simple Western Technology simplifies and automates the traditional western blotting process by integrating it into a fully automated capillary system, providing more precise quantitative data than traditional western blotting due to its controlled and standardized operating conditions. </p><ul><li>Simple Western is a fully automated western technology. Traditional western blotting steps of sample separation, wash buffer incubations, and immunodetection of target proteins occur inside a hands-free instrument on your benchtop. </li><li>Unlike traditional western blotting systems, Simple Western does not transfer proteins to a membrane, removing the inherent variability of western blotting and adding high-throughput sample processing and automated protein analysis. </li><li>Tiny amounts of sample are separated inside capillaries and are cross linked to the capillary walls. Then, blocking and wash buffer incubations are performed using just a few microliters of your primary antibodies and conjugated secondary antibodies to detect target proteins in chemiluminescence and NIR/IR fluorescence channels (LOD <1 pg). </li></ul></div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_indv_media"> <div> <article> <div class="youtube-video-place"> <lite-youtube videoid="TQ1GiX2qKV8" params="rel=0" style="background-image: url('https://img.youtube.com/vi/TQ1GiX2qKV8/maxresdefault.jpg');"> <a href="https://www.youtube.com/embed/TQ1GiX2qKV8?autoplay=1" class="lty-playbtn" title="How Simple Western Technology works"> <span class="lyt-visually-hidden">How Simple Western Technology works</span> </a> </lite-youtube> </div> </article> </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--button-row paragraph--view-mode--default"> <div class="row"> <div class="col"> <div class="list-grid-wrap"> <div class="row"> <div class="col-md-4"> <a href="/instruments/simple-western/models" class="btn btn-primary" type="link"> Compare Available Models </a> </div> <div class="col-md-4"> <a href="https://embed.envoke-demos.com/bio-techne/leo_platform/web.html" class="btn btn-primary" type="link"> Take Leo 3D Tour </a> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--clickable-icons-container paragraph--view-mode--default"> <div class="container"> <div class="row"> <div class="col p-0 text-left"> <h2>Explore Simple Western</h2> </div> </div> <div class="row"> <div class="col text-left"> </div> </div> <div class="row"> <div class="row justify-content-start"> <div class="col-xl-2 col-lg-2 col-md-4 col-sm-6 col-6 mb-4"> <div class="paragraph paragraph--type--indvidual-clickable-icon paragraph--view-mode--default card p-3 text-center h-100 mb-0"> <a href="/instruments/simple-western/models" > <div><article> <div><img src='https://resources.bio-techne.com/bio-techne-assets/images/research-areas/lg-all-models-icon-534x534.png' alt='icon of simple western instrument'></div> </article> </div> <p><strong>See All Models</strong></p> </a> </div> </div> <div class="col-xl-2 col-lg-2 col-md-4 col-sm-6 col-6 mb-4"> <div class="paragraph paragraph--type--indvidual-clickable-icon paragraph--view-mode--default card p-3 text-center h-100 mb-0"> <a href="/instruments/simple-western/simple-western-assays" > <div><article> <div><img src='https://resources.bio-techne.com/bio-techne-assets/images/research-areas/lg-consumables-icon-534x534.png' alt='consumables icon'></div> </article> </div> <p><strong>Assays and Consumables</strong></p> </a> </div> </div> <div class="col-xl-2 col-lg-2 col-md-4 col-sm-6 col-6 mb-4"> <div class="paragraph paragraph--type--indvidual-clickable-icon paragraph--view-mode--default card p-3 text-center h-100 mb-0"> <a href="/resources/simple-western-antibody-database" > <div><article> <div><img src='https://resources.bio-techne.com/bio-techne-assets/images/research-areas/lg-validated-antibody-icon-534x534.png' alt='validated antibody icon'></div> </article> </div> <p><strong>Validated Antibodies</strong></p> </a> </div> </div> <div class="col-xl-2 col-lg-2 col-md-4 col-sm-6 col-6 mb-4"> <div class="paragraph paragraph--type--indvidual-clickable-icon paragraph--view-mode--default card p-3 text-center h-100 mb-0"> <a href="/resources/instrument-software-download-center/compass-software-simple-western" > <div><article> <div><img src='https://resources.bio-techne.com/bio-techne-assets/images/research-areas/lg-software-icon-534x534.png' alt='software computer icon'></div> </article> </div> <p><strong>Software</strong></p> </a> </div> </div> <div class="col-xl-2 col-lg-2 col-md-4 col-sm-6 col-6 mb-4"> <div class="paragraph paragraph--type--indvidual-clickable-icon paragraph--view-mode--default card p-3 text-center h-100 mb-0"> <a href="/resources/instrument-citations?instrument-platform=simple%20western" > <div><article> <div><img src='https://resources.bio-techne.com/bio-techne-assets/images/research-areas/articles-light-blue-icon-534x534.png' alt='Articles'></div> </article> </div> <p><strong>Citations</strong></p> </a> </div> </div> <div class="col-xl-2 col-lg-2 col-md-4 col-sm-6 col-6 mb-4"> <div class="paragraph paragraph--type--indvidual-clickable-icon paragraph--view-mode--default card p-3 text-center h-100 mb-0"> <a href="https://resources.bio-techne.com/bio-techne-assets/docs/pdfs/br-simple-western-product-brochure.pdf" > <div><article> <div><img src='https://resources.bio-techne.com/bio-techne-assets/images/research-areas/lg-citations-icon-534x534.png' alt='citation icon'></div> </article> </div> <p><strong>Brochure</strong></p> </a> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--section paragraph--view-mode--default mb-4"> <div> <div> <div class="paragraph paragraph--type--_-2-text-to-media mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_header_2_global"> <div><h2>How Can Simple Western Help You?</h2></div> </div> <div class="field_block:paragraph:2_2_text_to_media:field_lg_sngl_frmtd_text_global"> <div><p><strong>Fully Automated.  </strong>Just load your samples and press start. Pair Simple Western with your automated liquid handler to prepare and load samples, reagents, antibodies, and wash buffer, and set up multiple plates for automated protein analysis.  </p><p><strong>Low Sample Volume.  </strong>Use as little as 3 µL of starting material and generate up to 8 data points with just 5 µL. </p><p><strong>High Sensitivity Multiplex.</strong>  Get industry-leading sensitivity in chemiluminescence and fluorescence channels and the advantage of flexible  <a href="/instruments/simple-western/multiplex-protein-analysis-simple-western" target="_blank" rel="noreferrer noopener">multiplex assays</a>. </p><p><strong>Fast Time to Results.  </strong>With a<strong> </strong>processing time of 3 hours, Simple Western dramatically speeds up time to results compared to other western blot processors. </p><p><strong>Reproducible and Quantitative.  </strong>By eliminating the variability from the gel-to-membrane transfer of western blotting protocols, Simple Western is highly reproducible and fully quantitative. Plus, you get total protein detection and built-in  <a href="/instruments/simple-western/automate-total-protein-normalization-with-simple-western" target="_blank" rel="noreferrer noopener">total protein normalization</a>. </p><p><strong>Save valuable samples.  </strong><a href="/instruments/simple-western/one-sample-two-western-immunoassay-replex-jess-abby" target="_blank" rel="noreferrer noopener">RePlex™</a><a href="/instruments/simple-western/one-sample-two-western-immunoassay-replex-jess-abby">  </a>assays on <a href="/p/simple-western/jess-automated-western-blot-system_004-650">Jess™</a>, <a href="/p/simple-western/abby_004-680">Abby™</a>  and <a href="/p/simple-western/leo-system_004-550">Leo™</a>  Systems allows you to get multiple data points per sample.  </p><p><strong>Choose from >5,000 validated antibodies. </strong>Explore the <a href="/resources/simple-western-antibody-database" target="_blank" rel="noreferrer noopener">Simple Western-validated antibodies</a> to find the antibodies for a specific research application.</p></div> </div> <div class="field_block:paragraph:2_2_text_to_media:field_call_to_action_global"> <a href="/p/instruments/simple-western/request-quote" class="btn btn-primary" type="link"> Request Pricing </a> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/lg-simple-western-western-blot-comparison-450x450-v3.png" alt="Simple Western vs Western Blot Infographic" loading="lazy" > </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--section paragraph--view-mode--default mb-4"> <div> <div> <div class="paragraph paragraph--type--_-2-text-to-media mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_header_2_global"> <div><h2>Move Beyond Traditional Western Blots</h2></div> </div> <div class="field_block:paragraph:2_2_text_to_media:field_lg_sngl_frmtd_text_global"> <div><p>Simple Western Technology is an automated capillary western combining protein separation by size or charge with <a href="/instruments/simple-western/stellar-nir-ir-fluorescence-detection-modules">sensitive chemiluminescence and fluorescence immunodetection</a> using conventional primary and conjugated secondary antibodies.  </p><h3>How it works</h3><ol><li><strong>Prepare your reagents and samples:</strong> Reagent prep with Simple Western isn’t complicated and prepare your samples as you would for a traditional western blot.  </li><li><strong>Load the plate:</strong> After preparing the reagents, simply load the reagents and samples into a Simple Western plate. </li><li> <strong>Start the run:</strong> Place the plate and a capillary cartridge into the Simple Western System and use Compass software to start a run. </li><li> <strong>Analyze your results:</strong> Simple Western does protein analysis through separation and immunoprobing in a capillary system. Each step of the process is precisely controlled, ensuring the highest quality results. Come back to fully analyzed, <a href="/instruments/simple-western/looking-for-reliable-quantitative-results-simple-western">quantitative results.</a>  </li></ol></div> </div> <div class="field_block:paragraph:2_2_text_to_media:field_call_to_action_global"> <a href="/instruments/simple-western/simple-western-assays-utilizing-size-based-separation" class="btn btn-primary" type="link"> Explore Simple Western Size-based Assays </a> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/simple-western-size-chemiluminescent-detection-140x250.png" alt="Simple Western Size Chemiluminescent Detection" loading="lazy" > </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--section paragraph--view-mode--default"> <div> <div> <div class="paragraph paragraph--type--card-paragraph paragraph--view-mode--default card-para-default"> <h2>Hear from your peers using Simple Western Technology</h2> <div class="row card_row"> <div class="col-md-4"> <div class="h-100"> <div class="d-flex flex-column h-100"> <a href="/resources/testimonials/protac-drug-discovery-jess" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: #f5f5f5; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/dachel-doidg-headshot-white-background.png" alt="dachel doidg headshot" loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="/resources/testimonials/protac-drug-discovery-jess" > <h3 class="card_title_htag"></h3> </a> <div class="card_description flex-grow-1"> <div><p><em>"I work for a fast-paced drug discovery CRO where our clients expect high-quality data with rapid turnarounds. Jess allows me to accurately assess drug compound potency, and with its high throughput ability I can screen multiple compounds quickly and efficiently."</em> </p><p><strong>Rachel Doidge Ph.D</strong>, Senior Research Scientist, Aurelia Bioscience, Biocity, Nottingham, UK </p></div> </div> <div class="card-bottom-link"> <div> <a href="/resources/testimonials/protac-drug-discovery-jess" > Read Their Story </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="d-flex flex-column h-100"> <a href="/resources/testimonials/unraveling-neurodegenerative-diseases-with-jess" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: #f5f5f5; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/nicolas-bazan-headshot-white-circle.png" alt="Nicolas G. Bazan Headshot " loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="/resources/testimonials/unraveling-neurodegenerative-diseases-with-jess" > <h3 class="card_title_htag"></h3> </a> <div class="card_description flex-grow-1"> <div><p><em>"It saves so much time! It takes away so much of the pipetting and manipulation error. You just load your samples and wait for results, and in the meantime, you can dedicate yourself to other activities."</em> </p><p><strong>Nicolas G. Bazan, MD, PhD</strong>, Director of Neuroscience Center of Excellence, School of Medicine, Louisiana State University Health New Orleans, New Orleans </p></div> </div> <div class="card-bottom-link"> <div> <a href="/resources/testimonials/unraveling-neurodegenerative-diseases-with-jess" > Read Their Story </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="d-flex flex-column h-100"> <a href="/resources/videos/hear-from-simple-western-users" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: #f5f5f5; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/icons/icon-video-content_light_blue.png" alt="video content" loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="/resources/videos/hear-from-simple-western-users" > <h3 class="card_title_htag"></h3> </a> <div class="card_description flex-grow-1"> <div><p>Researchers reveal why they use Simple Western and how it is accelerating their research.</p></div> </div> <div class="card-bottom-link"> <div> <a href="/resources/videos/hear-from-simple-western-users" > Learn More </a> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><h2><strong>Find Simple Western Optimized Protocols & Methods</strong> </h2><p>Simple Western Platforms have been utilized in various research areas from cancer biology, neuroscience to drug discovery and development. Discover how this technology is advancing many scientific disciplines below with optimized methods.</p></div> </div> </div> <div> <div class="paragraph paragraph--type--card-paragraph paragraph--view-mode--default card-para-default"> <div class="row card_row"> <div class="col-md-4"> <div class="h-100"> <div class="d-flex flex-column h-100"> <h3 class="card_title_htag"></h3> <div class="card_description flex-grow-1"> <div><p><a href="/resources/instrument-applications/accelerate-your-cell-gene-therapy-workflows-with-advanced-western-blotting-solutions"><strong>Cell & Gene Therapy</strong></a></p><ul><li>Potency Assays </li><li>Viral Titer </li><li>Capsid Protein Ratio </li><li>Empty/Full Content Ratio </li><li>Process-Related Impurities </li><li>Viral Identity </li></ul></div> </div> <div class="card-bottom-link"></div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="d-flex flex-column h-100"> <h3 class="card_title_htag"></h3> <div class="card_description flex-grow-1"> <div><p><a href="/resources/instrument-applications/advanced-western-blotting-solutions-for-targeted-protein-degradation"><strong>Targeted Protein Degradation</strong></a></p><ul><li>Validating targets </li><li>DC<sub>50</sub></li></ul></div> </div> <div class="card-bottom-link"></div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="d-flex flex-column h-100"> <h3 class="card_title_htag"></h3> <div class="card_description flex-grow-1"> <div><p><a href="/resources/instrument-applications/extracellular-vesicle-protein-analysis-with-jess"><strong>Extracellular Vesicles</strong></a></p><ul><li>EV Protein Expression</li><li>EV Protein Markers</li></ul></div> </div> <div class="card-bottom-link"></div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="d-flex flex-column h-100"> <h3 class="card_title_htag"></h3> <div class="card_description flex-grow-1"> <div><p><a href="/resources/instrument-applications/bioprocess-contaminant-detection-using-simple-western"><strong>Bioprocess Impurity Detection</strong></a></p><ul><li>Host cell protein (HCP) contamination </li><li>Residual proteins</li></ul></div> </div> <div class="card-bottom-link"></div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="d-flex flex-column h-100"> <h3 class="card_title_htag"></h3> <div class="card_description flex-grow-1"> <div><p><a href="/resources/instrument-applications/advanced-western-blotting-solutions-for-vaccine-development"><strong>Vaccine Development</strong></a></p><ul><li>Serum Antibody Measurements </li><li>Contaminant Detection </li><li>Vaccine Efficacy </li><li>Monitoring Vaccine Stability</li></ul></div> </div> <div class="card-bottom-link"></div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="d-flex flex-column h-100"> <h3 class="card_title_htag"></h3> <div class="card_description flex-grow-1"> <div><p><a href="/resources/instrument-applications/high-throughput-pharmacokinetic-analysis-with-simple-western"><strong>Pharmacokinetic Analysis</strong></a></p><ul><li>Quantify intact IgG, aggregates, and fragments </li><li>IC/EC<sub>50</sub> </li><li>LOD </li><li>LOQ</li></ul></div> </div> <div class="card-bottom-link"></div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="d-flex flex-column h-100"> <h3 class="card_title_htag"></h3> <div class="card_description flex-grow-1"> <div><p><a href="/resources/instrument-applications/advanced-western-blotting-solutions-for-oncology"><strong>Cancer Biology</strong></a></p><ul><li>Immune checkpoints & hypoxia </li><li>Tumor microenvironment </li><li>Low abundant protein</li></ul></div> </div> <div class="card-bottom-link"></div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="d-flex flex-column h-100"> <h3 class="card_title_htag"></h3> <div class="card_description flex-grow-1"> <div><p><a href="/resources/instrument-applications/advanced-western-blotting-solutions-for-cell-signaling"><strong>Cell Signaling Pathways</strong></a></p><ul><li>Phosphorylation events & isoforms </li><li>High sensitivity multiplex assays</li></ul></div> </div> <div class="card-bottom-link"></div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="d-flex flex-column h-100"> <h3 class="card_title_htag"></h3> <div class="card_description flex-grow-1"> <div><p><a href="/resources/instrument-applications/advanced-western-blotting-applications-for-sars-cov-2-research"><strong>SARS-CoV-2 Research</strong></a></p><ul><li>SARS-CoV-2 Serological assay </li><li>SARS-CoV-2 Multi-antigen serological assay </li><li>ACE2/TMPRSS2</li></ul></div> </div> <div class="card-bottom-link"></div> </div> </div> </div> </div> </div> </div> </div> </div> </article> </div> </div> </section> </main> </div> <div id="brandsbar-footer" class="bg-bt-primary"> <div class="container-wide 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