Investigation of Cytotoxic Compounds in Ethyl Acetate and Chloroform Extracts of Nigella sativa Seeds by Sulforhodamine-B Assay-Guided Fractionation

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Compounds in Ethyl Acetate and Chloroform Extracts of Nigella sativa Seeds by Sulforhodamine-B Assay-Guided Fractionation</title> <meta name="description" content="Investigation of Cytotoxic Compounds in Ethyl Acetate and Chloroform Extracts of Nigella sativa Seeds by Sulforhodamine-B Assay-Guided Fractionation"> <meta name="keywords" content="cytotoxic compounds, half-maximal inhibitory concentration, high-performance liquid chromatography, human HepG2 cells, nigella sativa seeds, Sulforhodamine-B assay"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link 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Dissanayaka</a> </p> <p class="card-text"><strong>Abstract:</strong></p> A Sulforhodamine-B assay-guided fractionation on Nigella sativa seeds was conducted to determine the presence of cytotoxic compounds against human hepatoma (HepG2) cells. Initially, a freeze-dried sample of Nigella sativa seeds was sequentially extracted into solvents of increasing polarities. Crude extracts from the sequential extraction of Nigella sativa seeds in chloroform and ethyl acetate showed the highest cytotoxicity. The combined mixture of these two extracts was subjected to bioassay guided fractionation using a modified Kupchan method of partitioning, followed by Sephadex庐 LH-20 chromatography. This chromatographic separation process resulted in a column fraction with a convincing IC50 (half-maximal inhibitory concentration) value of 13.07碌g/ml, which is considerable for developing therapeutic drug leads against human hepatoma. Reversed phase High-Performance Liquid Chromatography (HPLC) was finally conducted for the same column fraction, and the result indicates the presence of one or several main cytotoxic compounds against human HepG2 cells. <iframe src="https://publications.waset.org/abstracts/144007.pdf" style="width:100%; height:400px;" frameborder="0"></iframe> <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=cytotoxic%20compounds" title="cytotoxic compounds">cytotoxic compounds</a>, <a href="https://publications.waset.org/abstracts/search?q=half-maximal%20inhibitory%20concentration" title=" half-maximal inhibitory concentration"> half-maximal inhibitory concentration</a>, <a href="https://publications.waset.org/abstracts/search?q=high-performance%20liquid%20chromatography" title=" high-performance liquid chromatography"> high-performance liquid chromatography</a>, <a href="https://publications.waset.org/abstracts/search?q=human%20HepG2%20cells" title=" human HepG2 cells"> human HepG2 cells</a>, <a href="https://publications.waset.org/abstracts/search?q=nigella%20sativa%20seeds" title=" nigella sativa seeds"> nigella sativa seeds</a>, <a href="https://publications.waset.org/abstracts/search?q=Sulforhodamine-B%20assay" title=" Sulforhodamine-B assay"> Sulforhodamine-B assay</a> </p> <a href="https://publications.waset.org/abstracts/144007/investigation-of-cytotoxic-compounds-in-ethyl-acetate-and-chloroform-extracts-of-nigella-sativa-seeds-by-sulforhodamine-b-assay-guided-fractionation" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/144007.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">400</span> </span> </div> </div> </div> </main> <footer> <div id="infolinks" class="pt-3 pb-2"> <div class="container"> <div style="background-color:#f5f5f5;" class="p-3"> <div 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Investigation of Cytotoxic Compounds in Ethyl Acetate and Chloroform Extracts of Nigella sativa Seeds by Sulforhodamine-B Assay-Guided Fractionation